CN105738622A - Human urine HIV1/2 antibody detection test paper through colloidal gold chromatographic method and preparation method thereof - Google Patents
Human urine HIV1/2 antibody detection test paper through colloidal gold chromatographic method and preparation method thereof Download PDFInfo
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract
The invention belongs to the field of medical immunologic application and discloses a human urine HIV1/2 antibody detection test paper through a colloidal gold chromatographic method and a preparation method thereof. The test paper is a test paper strip which is formed by successively laminating and pasting a sample pad, a gold-coated polyester film, a coated nitrocellulose membrane and a water-absorbing pad surface on a substrate board, wherein a detection line and a quality control line are formed on the coated nitrocellulose membrane. In a detection zone of the nitrocellulose membrane, an HIV-1 gp41 antigen and an HIV-2 gp36 antigen are coated, and in the quality control zone, a goat-anti-human IgG polyantibody is coated. During detection, 200 [mu]l of urine from scrub acceptor by means of a 200 [mu]l transfer pipette, and the test paper strip is inserted into a test tube to detect the urine. In the invention, the specific antigens, HIV-2 gp36 and HIV-1 gp41, of AIDS are introduced into the HIV1/2 antibody quick detection test strip, and optimization process on the sample pad is improved, so that the detection test paper can achieve detection of the HIV1/2 antibody in human urine. The test paper has high sensitivity, specificity and accuracy, is improved in adaptability and is convenient to use.
Description
Technical field
The invention belongs to medical immunology application, be specifically related to utilize HIV1/2 antibody in colloidal gold chromatographic technology for detection human urine
Reagent paper and preparation method thereof.
Background technology
HIV (human immunodeficiency virus) infection is in ascendant trend year by year, and State Council highlights and " expands AIDS detection service area, promote the use of quick, easy inspection
Survey method, improves detection accessibility ", thus effectively prevent and treat acquired immune deficiency syndrome (AIDS).Detection coverage rate is expanded further while avoiding occupational exposure risk,
Simplicity, noinvasive, safe and effective AIDS have become trend of the times from check reagent.
The each phase patient urine carried HIV contains the specific antibody of anti HIV-1 virus, antibody concentration and resisting in serum in urine
Body level is consistent, and urine has the advantages such as sampling convenience, noinvasive, nothing infectivity.Therefore, blood testing HIV antibody is replaced gradually with urine
Become a kind of brand-new means of HIV detection.
Develop the inspection such as urine examination HIV-1 antibody ELISA immunoadsorption (ELISA) and protein immunoblot (Western Blot) both at home and abroad successively
Test agent, its sensitivity and specificity are suitable with HIV blood examination reagent, but its testing cost is higher, and need special experiment condition, are unsuitable for advising greatly
Mould is promoted.
Simple and rapid HIV urinalysis reagent becomes the new direction of people's research, owing in urine, antibody content is low, the only one thousandth of blood
Even ten thousand/, the sensitivity to urinalysis reagent is had higher requirement.
Select highly sensitive detection method, or improve the accuracy of detection by improving technique, and easy, quickly detection and reduction
Cost is requirement and the direction of acquired immune deficiency syndrome (AIDS) urinalysis reagent development.
Summary of the invention
The present invention develops a new HIV measuring technology, i.e. a kind of colloidal gold chromatography detection human urine HIV1/2 antibody reagent paper, this skill
Art engages existing various technology, can carry out fast urine test, with experimenter more receptive, simple, non-intrusion type, safety,
Method of testing replaces traditional blood testing method fast and accurately, is beneficial to identify more HIV person, thus realizes early finding, early
Treatment, accurate and safe non-intrusion type collection of specimens, strengthen state control and the ability of monitoring HIV.
One of the object of the invention is to provide a kind of colloidal gold chromatography detection human urine HIV1/2 antibody reagent paper.
The two of the object of the invention are to provide the preparation method of a kind of colloidal gold chromatography detection human urine HIV1/2 antibody reagent paper.
A kind of colloidal gold chromatography detection human urine HIV1/2 antibody reagent paper, including celluloid coated film (1), colloidal gold conjugate pad
(4), sample pad (5), adsorptive pads (6), they are pasted onto on base plate successively, it is characterised in that:
Detection line (2) and nature controlling line (3) is had on described celluloid coated film (1),
The preparation method of the test strips of a kind of colloidal gold chromatography detection human urine HIV1/2 antibody, this reagent paper is coated by sample pad, gold
Polyester film, coated nitrocellulose filter, adsorptive pads collectively constitute, it is characterised in that the method includes following steps:
Step 1, the preparation of antigen: select the HIV1/2 virus recombinant antigen of the gene engineering expression of purification,
And the preparation of coated nitrocellulose filter: be coated basal liquid dilution antigen to concentration be respectively 0.6-1.76mg/ml and
0.2mg/ml, is sprayed on nitrocellulose filter and is dried 18～20 hours, and in sealing the aluminium foil bag being contained in desiccant, pasting board is used.
And the preparation of golden coated polyester film pad: redissolve (solution 1) with gold conjugate suspension, gold conjugate absorption value is adjusted to 30
-34, continue three absorption values of detection.With the filtering with microporous membrane of 0.22ul, 2～8 DEG C of preservations of labelling are stand-by.
The preparation of solution 1: measure 1200ml purified water in clean glass container, heats on heating stirrer, measures purification before boiling
Water dissolution has weighed the sodium citrate that ratio is 0.00035g/ml.The gold chloride of the 4% of the lower additional proportion 0.0045 of little shelves stirring after boiling completely,
Taking sample detection, it is desirable in 520-525nm wave-length coverage, maximum light absorption value is 1.70 to 2.30.By the most qualified gold colloidal regulation PH extremely
6.0～7.0, the lower Protein A solution 1.5～1.8ml that adds of little shelves stirring, at least stirring 20～30 minutes, detects absworption peak, absorption value.Add
The BSA of 10% continues stirring at least 30 minutes, detection absworption peak and absorption value.Under 5 DEG C～15 DEG C of cryogenic conditions, 8000-10000rpm/min
Centrifugal 50～60 minutes, carefully draw supernatant, blot supernatant as far as possible.
And gold colloidal is coated the preparation of conjugate: accurate measuring colloidal gold conjugate, add sucrose being allowed to content is 20% and trehalose is allowed to
Content is 5%, is inverted mixing gently, adds 10%UHP (15ul/ml) and be inverted mixing gently before being coated beginning.With the package amount of 0.7ul/mm,
The axle countershaft Accuflow machine of being coated is used to be coated.50 DEG C of 2h ± 15min are sealed in the aluminium foil bag containing desiccant after drying, are stored in drying chamber.
And the preparation of sample pad, it is the glass fibre membrane of treated liquid impregnation process, standby after drying in 37 DEG C；
Step 2, on base plate, sequentially mutually overlap joint is pasted sample pad, the golden coated polyester film through completing made by abovementioned steps 1, is coated
Nitrocellulose filter, adsorptive pads；
Step 3, to the material completed made by step 2, cuts into test strips.
In described step 3, the width of the trial-production bar cut into is preferably >=3.5mm.
The Cleaning Principle of the present invention is to be to use colloid gold immune detection technique, and fast qualitative measures the HIV1/2 type in human urine sample and resists
Body.This product uses the protein A (Au-PA) of pre-coated colloid gold label, detects and be coated Recombinant HIV respectively at line and control line on nitrocellulose filter
Antigen (HIV-Ag) and goat anti-human igg.During detection positive sample, in sample, HIV antibody is combined formation complex with colloid gold label protein A, by
Move forward along paper slip in chromatography effect complex, be combined formation through detection line with pre-coated Recombinant HIV-Ag
" Au--PA-HIV-Ab-HIV (1+2)-Ag " complex and condense colour developing, free colloid gold label restructuring Au-PA is then at control line and goat-anti
Human IgG F (ab)2Segment combines and is enriched with colour developing.' negative ' specimens the most only develops the color at control line, observed result in 20～45 minutes.
The preparation method of colloidal gold chromatography of the present invention detection human urine HIV1/2 antibody reagent paper:
Step 1, the preparation of dosing
The preparation of 1.4% chlorogold solution
The purified water of joined total amount of liquid 80% can measure with graduated cylinder, and remainder uses pipet to add, when the weight of purified water is near the mark
Significant care is wanted, if it is desired, less transfer pipette can be used instead to ensure that the error of target weight is within ± 2% during amount.With 0.22ul's
It is sub-packed in brown bottle after filtering with microporous membrane amount volume, labelled lucifuge 2～8 DEG C of preservations.
The preparation of 2.10% bovine serum albumin solution
Accurately weigh 10g BSA to add in 80ml purified water, be stirred at room temperature more than 5 minutes, fully mix, purified water constant volume to 100ml,
It is again stirring for 5 minutes, fully mixes.With the filtering with microporous membrane of 0.22um, after amount volume in installed reagents bottle, labelled 2～8 DEG C of preservations.
3.0.2M the preparation of solution of potassium carbonate
Accurately weigh 2.76g K2CO3Add in 80ml purified water, be stirred at room temperature more than 15 minutes, fully mix, purified water constant volume to 100ml,
It is again stirring for, more than 5 minutes, fully mixing.In subpackage reagent bottle, labelled room temperature preservation.
4. the preparation of gold label buffer suspension liquid
Disodium hydrogen phosphate, sodium dihydrogen phosphate, sodium chloride, BSA order are added in purified water, are stirred at room temperature more than 15 minutes, fully mix,
Purified water constant volume, to 100ml, is again stirring for, more than 5 minutes, fully mixing, in subpackage reagent bottle, and labelled 2～8 DEG C of preservations.
5. the preparation of Protein A solution (5mg/ml)
Calculate the amount of protein A: the consumption of (preparing volume × 5) ÷ material protein A concentration=should add protein A
Glycerol consumption: the consumption of the volume of cumulative volume-protein A=add 50% glycerol.
By consumption and the consumption mixing of protein A of above-mentioned 50% glycerol, repeatedly it is inverted fully mixing, labelled 2～8 DEG C of preservations.
The preparation of 6.50% glycerite
The glycerol measuring 50ml adds in 30ml purified water, is inverted fully mixing, and purified water constant volume, to 100ml, fully mixes, and subpackage tries
In agent bottle, labelled 2～8 DEG C of preservations.
The preparation of 7.50% sucrose solution
Accurately weigh 50g sucrose, add in 80ml purified water, be stirred at room temperature more than 15 minutes, fully mix, purified water constant volume to 100ml,
It is again stirring for, more than 5 minutes, fully mixing.In subpackage reagent bottle, labelled 2-8 DEG C of preservation.
The preparation of 8.1M potassium bicarbonate solution
Accurately weigh 10.012g KHCO3Adding in 80ml purified water, be stirred at room temperature more than 15 minutes, fully mix, purified water constant volume arrives
100ml, is again stirring for, more than 5 minutes, fully mixing.In subpackage reagent bottle, labelled room temperature preservation.
The preparation of 9.10%T904 solution
Weigh 10g T904 to add in 80ml purified water, be stirred at room temperature at least 15 minutes, fully mix, purified water constant volume to 100ml, then
Secondary stirring is more than 5 minutes, fully mixes.In subpackage reagent bottle, labelled 2～8 DEG C of preservations.
The preparation of 10.10%Tween-80 solution
Measure 10ml Tween-80 to add in 80ml purified water, be stirred at room temperature more than 15 minutes, fully mix, purified water constant volume to 100ml,
It is again stirring for, more than 5 minutes, fully mixing.In subpackage reagent bottle, labelled 2～8 DEG C of preservations.
11.0.1%Tween-80 the preparation of solution
Measuring 1ml 10%Tween-80 to add in 80ml purified water, be stirred at room temperature more than 15 minutes, fully mix, purified water constant volume arrives
100ml, is again stirring for, more than 5 minutes, fully mixing.In subpackage reagent bottle, labelled 2～8 DEG C of preservations.
The preparation of 12.10% TritonX-100 solution
Measure 10ml TritonX-100 to add in 80ml purified water, be stirred at room temperature more than 15 minutes, fully mix, purified water constant volume to 100ml,
It is again stirring for, more than 5 minutes, fully mixing.In subpackage reagent bottle, labelled 2～8 DEG C of preservations.
Prepared by step 2. protein A-colloidal gold
Measure 1200ml purified water in clean glass container, heating stirrer heats, measured 25ml purified water before boiling and dissolved
The sodium citrate 0.42g weighed.Completely after boiling the stirring of little shelves lower add 4% gold chloride 5.5ml-6.5 ,-secondary rapidly join the Fructus Citri Limoniae dissolved
Acid sodium continues stirring 10 minutes.Put into stirring in cold water and take sample detection, it is desirable in 520～525nm wave-length coverages to room temperature in 30 minutes,
Maximum light absorption value is 1.70 to 2.30.Otherwise discard and again prepare.The most qualified gold colloidal is adjusted PH to 6.0-7.0, under the stirring of little shelves, adds egg
White solution A (5mg/ml) 1.5ml, at least stirring 30 minutes, detects absworption peak, absorption value.The BSA adding 10% continues stirring at least 30
Minute, detect absworption peak, absorption value.5 DEG C of-15 DEG C of 8000-10000rpm/min are centrifuged 50 minutes, carefully draw supernatant, blot supernatant as far as possible.
Redissolve with gold conjugate suspension, 1: 40 dilution detection absorption value (detecting three times), gold conjugate absorption value is adjusted to 30～34, continues detection
Three absorption values.With the filtering with microporous membrane of 0.22ul, 2～8 DEG C of preservations of labelling are stand-by.
The coated preparation of step 3. colloidal gold conjugate
Accurate measuring colloidal gold conjugate, add sucrose be allowed to content be 20% and trehalose to be allowed to content be 5%, be inverted mixing gently, be coated
Add 10%UHP (15ul/ml) before starting and be inverted mixing gently.With the package amount of 0.6-0.7ul/mm, the Accuflow machine of being coated is used to be coated.
50 DEG C of 2h ± 15min are sealed in the aluminium foil bag containing desiccant after drying, are stored in drying chamber.
The coated preparation of step 4. NC Nitroncellulose film
1. the preparation of quality control reagent
Goat anti-human igg F (ab)2The calculating of multi-resistance usage amount
Concentration=the usage amount of (preparing volume × 0.2-0.25) ÷ goat anti-human igg F (ab) 2 multi-resistance
By 1M potassium carbonate, by 50% sucrose solution, methanol, purified water sequence described above preparation quality control reagent, mix with whirlpool agitator.Protect
It is stored in 2～8 DEG C.
2. the preparation of detectable
The computational methods of HIV-1 antigen addition
The amount that (preparation amount × 0.6-1.76) ÷ antigen concentration=antigen adds
The computational methods of HIV-2 antigen addition
The amount that (preparation amount × 0.2) ÷ antigen concentration=antigen adds
By 0.1%Tween-80 solution, 50% sucrose solution, methanol, HIV-1 antigen, HIV-2 antigen, the preparation inspection of purified water sequence described above
Test agent, mixes with whirlpool agitator.It is stored in 2～8 DEG C.
Step 5. quality control reagent, the coated preparation of detectable
1. discharge rate screening
It is coated sample (10) by 0.04～0.08ul/mm discharge rate, is dried 12～20 hours at the horizontal wet drying baker of 40 ± 2 DEG C of constant temperature.Pass through
Enterprise's internal control reference material sieve initial option is coated concentration.
2. being coated of NC Nitroncellulose film
Blank NC Nitroncellulose film is cut into 310mm ± 5mm bar, uses the machine that is coated will to examine line reagent and the quality control reagent discharge rate by screening
It is coated the corresponding position of NC Nitroncellulose film.It is dried 12～20 hours at 40 ± 2 DEG C of constant temperature and humidity drying casees.It is sealed to the aluminium foil bag containing desiccant
In, it is stored in drying chamber.
The process of step 6. sample pad
1. sample pad confining liquid preparation
Take 520 milliliters of ultra-pure waters of purified water in a clean container, add chicken serum after heat inactivation, potassium bicarbonate, three hypophosphite monohydrate hydrogen dipotassiums,
More than 10%T904 reagent, is dissolved in purified water the potassium stannate solution making 0.1M by potassium stannate, joins adjustment PH in the ratio of 1: 100
The solution of value fully mixes, is stirred at room temperature more than 15 minutes, fully mixes, adjust pH to 8.2～8.5, again prepare after discarding as gone beyond the scope.
Add purified water to 1000ml, with the filtering with microporous membrane of 0.22ul, standby.
2. process sample pad
20%UHP prepares: weighs UHP 8g and adds ultra-pure water to 40ml.
Glass fibre element film is pressed the cutting of 310mm/ bar, by UHP (every liter of sample pad confining liquid adds 25ml 10%UHP solution) before closing.Press
Every glass fibre membrane adds the standard of 5ml sample pad confining liquid, processes glass fibre membrane.
Processed glass fibre membrane drying at room temperature (temperature: 25 ± 5 DEG C, humidity: less than 28%) 8 hours (± 15 minutes), is dried knot
It is positioned over after bundle in the couveuse containing desiccant and humidity indicating card, is dried 12～18 hours in 50 ± 5 DEG C of constant temperature and humidity drying casees.It is sealed to
In aluminium foil bag containing desiccant, it is stored in drying chamber.
The assembling of step 8. card
In the relative humidity dust proof workshop less than 30%, it is assembled into card.
Assemble component have be coated nitrocellulose filter, adsorptive pads, protein A colloidal gold pad, close sample pad, gold pad cover)
Step 9. cutting, pack
In the relative humidity dust proof workshop less than 30%, the card cutting cutter being completed is cut into >=wide for 3.5mm test strips, by test strips
And desiccant loads heat sealing in aluminium foil bag together.
Step 10. assembles, packs
Being above the description of this invention and non-limiting, other embodiment based on inventive concept, all among protection scope of the present invention.
Claims (11)
1. a reagent paper for colloidal gold chromatography detection human urine HIV1/2 antibody, is pasted onto on base plate successively including sample pad, golden coated polyester film, coated nitrocellulose filter, adsorptive pads, it is characterised in that:
Described pad is coated with specific antigen (HIV-1 gp36) and specific antigen (HIV-1 gp41),
Detection line (T) and nature controlling line (C) is had on described celluloid coated film,
Detection line therein is coated with " IgG-albumen L-gold " conjunction type antigen protein, and nature controlling line therein (C) is coated with antibody (c).
The reagent paper of a kind of colloidal gold chromatography the most according to claim 1 detection human urine HIV1/2 antibody, it is characterized in that: during detection, the IgG in sample combines with albumen L-colloidal gold particle, form " IgG-albumen L-gold " conjugate, " IgG-albumen L-gold " conjugate is moved upward along test strips, arrives first at the detection zone being coated HIV antigen.If the antibody containing HIV in sample, synantigen is combined, and is detained on detection line, forms a pink line by " IgG-albumen L-gold " conjugate, and this represents positive findings.Represent that free " IgG-albumen L-gold " conjugate continues to move above reagent paper, arrives the check plot of reagent paper without HIV antibody in sample without colored lines in detection zone." IgG-albumen L-gold " conjugate will combine with goat anti-human igg's multi-resistance and be enriched on control line, form a pink line.The appearance of control line proves that ELISA test strip function is normal.No matter whether containing HIV-1/2 antibody in sample, in efficiency test, all should there is the light red control line to purple in the check plot of test strips.
The reagent paper of a kind of colloidal gold chromatography the most according to claim 1 detection human urine HIV1/2 antibody, it is characterised in that: having detection line and control line, detection line to be coated HIV1/2 antigen on coated nitrocellulose filter, control line is coated goat anti-human igg's multi-resistance.
The reagent paper of a kind of colloidal gold chromatography the most according to claim 1 detection human urine HIV1/2 antibody, it is characterized in that: described sample pad is to close, through sample pad, the glass fibre element film that immersion system processes, described sample pad treatment fluid is containing chicken serum, the PBS solution of BSA, and contains carbamide peroxide (UHP) oxidation impurities and Tween 80 and 904 surfactants.
The reagent paper of a kind of colloidal gold chromatography the most according to claim 1 detection human urine HIV1/2 antibody, it is characterised in that: the genetic engineering recombinant antigen that specific antigen HIV-1 gp41, HIV-1 gp41 are purification of coated HIV1/2 antigen.
The reagent paper of a kind of colloidal gold chromatography the most according to claim 1 detection human urine HIV1/2 antibody, it is characterized in that: being coated the nitrocellulose filter of use is slower 180 films of capillary flow rate, so can increase antibody and the response time of antigen on coated film in sample.
7. the preparation method of the test strips of a colloidal gold chromatography detection human urine HIV1/2 antibody, described in this trial-production claim 1-6 as the aforementioned, this trial-production is collectively constituted by sample pad, golden coated polyester film, coated nitrocellulose filter, adsorptive pads face, it is characterised in that the method includes:
Step 1, the preparation of antigen: select the HIV1/2 virus recombinant antigen of the gene engineering expression of purification,
And the preparation of coated nitrocellulose filter: it is respectively 1.76mg/ml and 0.2mg/ml with being coated basal liquid dilution antigen to concentration, is sprayed on nitrocellulose filter and is dried 18～20 hours, seal pasting board use in the aluminium foil bag being contained in desiccant,
And the preparation of golden coated polyester film pad: redissolve with gold conjugate suspension, gold conjugate absorption value is adjusted to 30～34, continue to detect three absorption values.With the filtering with microporous membrane of 0.22ul, 2～8 DEG C of preservations of labelling are stand-by,
And the coated preparation of colloidal gold conjugate: accurate measuring colloidal gold conjugate, add sucrose be allowed to content be 20% and trehalose to be allowed to content be 5%, be inverted mixing gently, add 10%UHP (15ul/ml) before being coated beginning and be inverted mixing gently.With the package amount of 0.7ul/mm, the axle countershaft Accuflow machine of being coated is used to be coated.Under the conditions of 50 DEG C, 2h ± 15min is sealed in the aluminium foil bag containing desiccant after drying, is stored in drying chamber,
And the preparation of sample pad, it is the glass fibre membrane of treated liquid impregnation process, standby after drying in 37 DEG C；
Step 2, on floor, sequentially mutually overlap joint pastes the sample pad through completing made by abovementioned steps 1, golden coated polyester film, coated nitrocellulose filter, adsorptive pads face；
Step 3, to the material completed made by step 2, cuts into test strips.
The reagent paper of a kind of colloidal gold chromatography the most according to claim 7 detection human urine HIV1/2 antibody, it is characterized in that: in described step 1, gold conjugate suspension manufacturing methods is for measuring 1200ml purified water in clean glass container, heating stirrer heats, measures purified water dissolving before boiling and weighed the sodium citrate that ratio is 0.00035g/ml.The gold chloride of the 4% of the lower additional proportion 0.0045 of little shelves stirring after boiling, takes sample detection, it is desirable in 520～525nm wave-length coverages completely, and maximum light absorption value is 1.70 to 2.30.By the most qualified gold colloidal regulation PH to 6.0～7.0, the lower Protein A solution 1.5～1.8ml that adds of little shelves stirring, at least stirring 20～30 minutes, detects absworption peak, absorption value.The BSA adding 10% continues stirring at least 30 minutes, detection absworption peak and absorption value.Under 5 DEG C～15 DEG C of cryogenic conditions, 10000rpm/min is centrifuged 50～60 minutes, carefully draws supernatant, blots supernatant as far as possible.
The reagent paper of a kind of colloidal gold chromatography the most according to claim 7 detection human urine HIV1/2 antibody, it is characterized in that: in described step 1, the process of sample pad, is added the standard of 5ml sample pad confining liquid by every glass fibre membrane, processes glass fibre membrane.Glass fibre membrane drying at room temperature (temperature: 25 ± 5 DEG C after process, humidity: less than 28%) 8 hours (± 15 minutes), it is dried after terminating and is positioned in the couveuse containing desiccant and humidity indicating card, be dried 12～18 hours in 50 ± 5 DEG C of constant temperature and humidity drying casees.It is sealed in the aluminium foil bag containing desiccant, is stored in drying chamber.
The reagent paper of a kind of colloidal gold chromatography the most according to claim 9 detection human urine HIV1/2 antibody, it is characterized in that: the preparation method of described sample pad confining liquid, for taking 520 milliliters of ultra-pure waters of purified water in a clean container, adds chicken serum, sodium bicarbonate, three hypophosphite monohydrate hydrogen dipotassiums, 10%T after above purified water, heat inactivation901Reagent was stirred at room temperature more than 15 minutes, fully mixed, and adjusted pH to 8.2～8.5, again prepared after discarding as gone beyond the scope.Potassium stannate is dissolved in purified water the potassium stannate solution making 0.1M, joins in the ratio of 1: 100 in the solution adjusting pH value and fully mix, add purified water to 1000ml, with the filtering with microporous membrane of 0.22ul. weigh UHP 8g and add ultra-pure water to 40ml.Glass fibre element film is pressed the cutting of 310mm/ bar, by UHP (every liter of sample pad confining liquid adds 25ml 10%UHP solution) before closing.
The preparation method of the test strips of 11. colloidal gold chromatography according to claim 7 detection human urine HIV1/2 antibody, it is characterised in that: in described step 3, the width of the trial-production bar cut into is preferably 4mm or 3mm two kinds.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106970219A (en) * | 2017-04-28 | 2017-07-21 | 北京金豪制药股份有限公司 | One kind is based on HIV in colloidal gold method detection urine（1+2）Antibody reagent |
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| CN111562366A (en) * | 2020-06-15 | 2020-08-21 | 郑州方欣生物科技有限责任公司 | Urine HIV antibody rapid detection kit, preparation method and use method |
| CN111766379A (en) * | 2020-07-09 | 2020-10-13 | 北京普赞生物技术有限公司 | Tissue sample extracting solution treatment method for improving color development of colloidal gold detection card |
| CN116626306A (en) * | 2023-05-18 | 2023-08-22 | 上海吉宣生物科技有限公司 | Preparation method of reagent strip for detecting HIV (l+2) antibody in urine, reagent strip and reagent pen |
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