CN105728069B - Multi-channel micro-fluidic chip for rapidly self-checking blood - Google Patents
Multi-channel micro-fluidic chip for rapidly self-checking blood Download PDFInfo
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- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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Abstract
The invention discloses a multi-channel micro-fluidic chip for rapidly self-checking blood, which comprises a substrate and a cover plate. The cover plate is integrated with a sample feeding channel, an inertia separation channel, a shunt channel, a plasma analysis channel and a blood cell analysis channel. The sample feeding channel, the inertia separation channel and the shunt channel are sequentially connected, and the shunt channel is connected to the plasma analysis channel and the blood cell analysis channel by adopting a Y-shaped structure. The sample introduction channel is used for inputting blood to be detected; the inertial separation channel separates the blood into plasma and blood cells, which flow into a shunt channel; the shunting channel introduces the plasma into the plasma analysis channel through the Y-shaped structure to carry out detection and analysis on the plasma, and introduces blood cells into the blood cell analysis channel to carry out detection and analysis on the blood cells. The invention adopts a highly integrated multi-channel micro-fluidic chip to realize various blood detection functions at one time on the basis of portability.
Description
Technical Field
The invention relates to the technical field of blood detection, in particular to a multi-channel micro-fluidic chip for quickly self-checking blood.
Background
The micro-fluidic chip is a small-sized analysis experiment platform integrating the functions of blood sample preparation, reaction, separation, detection and the like. The micro-fluidic chip analysis uses a micron-scale chip as an operation platform to complete the whole process of automatic analysis, and the device is characterized by mainly having an effective structure for accommodating fluid, such as a channel or a reaction chamber, and having strong integration, and the advantages of light volume, less used samples, high reaction speed, high flux, low pollution, and being disposable, and the like, thereby showing great development potential in the research fields of crossing subjects such as biology, chemistry, medicine, electronics, machinery and the like, and being used in numerous fields such as biomedical research, drug synthesis screening, environmental monitoring and protection, health quarantine, judicial identification, biological reagent detection and the like.
The microfluidic chip can bear various unit technologies, and the characteristics of flexible combination and scale integration of the unit technologies make the microfluidic chip become an important platform for various system researches, the application of the microfluidic chip in cell biology is continuously expanded, and the microfluidic chip is a future development direction in the field of biomedical analysis. With the development of the society at present, the concept of family medical care is continuously deepened into the heart, various household medical products are continuously released, however, the basic health examination content of blood detection still depends on diagnosis and treatment equipment of hospitals, the diagnosis and treatment equipment cannot realize various disposable blood detection functions, and the medical equipment is large in size and inconvenient to carry, so that the operation of family self-checking blood is difficult, and inconvenience is brought.
Disclosure of Invention
The invention mainly aims to provide a multi-channel micro-fluidic chip for quickly self-checking blood, and aims to solve the problems that the existing diagnosis and treatment equipment cannot realize various disposable blood detection functions and is inconvenient to carry.
In order to achieve the above object, the present invention provides a multi-channel micro-fluidic chip for rapid self-testing of blood, comprising a substrate and a cover plate, wherein a sample injection channel, an inertial separation channel, a shunt channel, a plasma analysis channel and a blood cell analysis channel are integrated in the cover plate, the sample injection channel, the inertial separation channel and the shunt channel are connected in sequence, the shunt channel is connected to the plasma analysis channel and the blood cell analysis channel by a Y-shaped structure, wherein:
the sample introduction channel is used for inputting blood to be detected;
the inertial separation channel separates the blood into plasma and blood cells, which flow into the diversion channel;
the shunting channel introduces the plasma into the plasma analysis channel through the Y-shaped structure to perform detection analysis on the plasma, and introduces the blood cells into the blood cell analysis channel to perform detection analysis on the blood cells.
Preferably, a waste liquid channel is further integrated in the cover plate, the plasma analysis channel and the blood cell analysis channel are both connected with the waste liquid channel, and the waste liquid channel is used for leading out waste liquid generated by analyzed plasma and blood cells.
Preferably, the plasma analysis channel comprises a hepatitis A analysis platform, a hepatitis B analysis platform, an arthritis analysis platform and a myocardial infarction analysis platform.
Preferably, the hepatitis a analysis platform comprises an IgM detection unit and an IgA detection unit; the hepatitis B analysis platform comprises an HBsAg detection unit, an HBsAb detection unit, an HBeAg detection unit, an HBeAb detection unit and an HBcAb detection unit; the arthritis analysis platform comprises an RF-IgM detection unit, an RF-IgA detection unit, an RF-IgG detection unit and an RF-IgE detection unit; the myocardial infarction analysis platform comprises an hs-CRP detection unit and a CK-MB detection unit; the immunoassay platform judges the detection result of the blood plasma by integrating the detection data of each detection unit in the immunoassay platform to the blood plasma.
Preferably, the blood cell analysis channel comprises a platelet counting platform, a red blood cell counting platform, a hemoglobin analysis platform, a white blood cell counting platform and a blood type analysis platform.
Preferably, the platelet counting platform comprises a platelet screen and a platelet counter; the red blood cell counting platform comprises a red blood cell sieve, a red blood cell drainage tube and a red blood cell counter; the platelet counting platform separates platelets in the hemocytes by controlling the size of the pipeline of the platelet sieve and counts by a platelet counter; the red blood cell counting platform separates red blood cells in the blood cells by controlling the size of the pipeline of the red blood cell sieve and counts the red blood cells by a red blood cell counter; the leucocyte counting platform separates and counts leucocytes in the leucocytes by controlling the size of a pipeline of the leucocyte counting platform.
Preferably, when blood cells flow through the blood cell analysis channel, the blood platelets are retained on the blood platelet counting platform, red blood cells pass through the red blood cell counting platform, enter the hemoglobin analysis platform through the red blood cell drainage tube for hemoglobin separation determination, and the remaining white blood cells are counted by the white blood cell counting platform and enter the blood type analysis platform for blood group genotype analysis.
Preferably, the hemoglobin analysis platform comprises a red blood cell lysis unit, a hemoglobin test unit and a valve, wherein:
the red blood cell lysis unit and the hemoglobin test unit are both disc structures and are vertically arranged;
the valve is arranged between the red blood cell lysis unit and the hemoglobin test unit;
the erythrocyte lysis unit is internally provided with a hemolytic agent.
Preferably, the sample introduction channel, the inertia separation channel, the shunt channel, the plasma analysis channel, the blood cell analysis channel and the waste liquid channel all adopt radian structures.
Compared with the prior art, the multi-channel micro-fluidic chip for rapidly and automatically detecting blood adopts the technical scheme, and the following beneficial effects are achieved: the multichannel microfluidic chip integrates the sample feeding channel, the separation area, the shunt channel, the analysis platform and the waste liquid channel on the centimeter-scale chip, so that various disposable blood detection functions are realized, and the multichannel microfluidic chip is small in size, convenient to carry and convenient to use for home self-checking.
Drawings
FIG. 1 is a schematic structural diagram of a preferred embodiment of a multi-channel microfluidic chip for rapid blood self-test according to the present invention;
FIG. 2 is a schematic structural diagram of a preferred embodiment of an inertial separation channel of the multi-channel microfluidic chip for rapid blood self-test according to the present invention;
FIG. 3 is a schematic structural diagram of a preferred embodiment of a plasma analysis channel of the multi-channel microfluidic chip for rapid self-test of blood according to the present invention;
FIG. 4 is a schematic structural diagram of a preferred embodiment of a blood cell analysis channel of the multi-channel microfluidic chip for rapid self-test of blood according to the present invention;
fig. 5 is a schematic structural diagram of a preferred embodiment of the hemoglobin measurement platform of the multi-channel microfluidic chip for rapid self-test of blood according to the present invention.
The objects, features and advantages of the present invention will be further explained with reference to the accompanying drawings.
Detailed Description
To further illustrate the technical means and effects of the present invention adopted to achieve the above objects, the following description will be made with reference to the accompanying drawings. It should be noted that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention in any way.
As shown in fig. 1, fig. 1 is a schematic structural diagram of a preferred embodiment of a multi-channel microfluidic chip for rapid self-test of blood according to the present invention. In this embodiment, the multi-channel microfluidic chip includes a substrate 1 and a cover plate 2, the cover plate 2 may be, but not limited to, made of Polydimethylsiloxane (PDMS), and the cover plate 2 integrates a microfluidic channel structure for implementing various blood detection functions, including a sample injection channel 21, an inertial separation channel 22, a shunt channel 23, a plasma analysis channel 24, a blood cell analysis channel 25, and a waste liquid channel 26. Sampling channel 21 adopts open pore structure to connect inertia separation channel 22, and this sampling channel 21 is used for linking to each other with blood transfusion tube and inputs and waits to examine blood. The inertia track 22 communicates with the inlet of the diversion track 23. The shunting passage 23 is a Y-shaped structure and includes an inlet and two outlets, and the two outlets of the shunting passage 23 are respectively connected to the plasma analysis passage 24 and the blood cell analysis passage 25. The plasma analysis channel 24 comprises a hepatitis A analysis platform 241, a hepatitis B analysis platform 242, an arthritis analysis platform 243 and a myocardial infarction analysis platform 244 which are arranged in parallel. The blood cell analysis channel 25 includes a platelet count platform 251, a red blood cell count platform 252, a hemoglobin analysis platform 253, a white blood cell count platform 254, and a blood type analysis platform 255. The plasma analysis channel 24 and the blood cell analysis channel 25 are both connected to the waste liquid channel 26, and the outlet of the waste liquid channel 26 is an open structure for leading out waste liquid generated by analyzed plasma and blood cells.
Fig. 2 is a schematic structural diagram of an inertial separation channel of a multi-channel microfluidic chip for rapid self-test of blood according to a preferred embodiment of the present invention, as shown in fig. 2. In this embodiment, a blood volume of microliter order is introduced into the sample introduction channel 21 by a capillary tube, the sample introduction channel 21 introduces the blood to be detected into the inertial separation channel 22, and the inertial separation channel 22 separates the blood into two parts of plasma and blood cells based on the inertial microflow principle. The schematic diagram of plasma separation in this embodiment is shown in fig. 2, the separation of plasma and blood cells in the blood is performed in the inertial separation channel 22, and the cross-sectional view of the inertial separation channel 22 shows that the inertial separation channel 22 is an asymmetric curved tubular structure, and the blood generates laminar flow by the flow of liquid in the tube when passing through the asymmetric curved tubular structure. Based on the asymmetry of the tube, the blood generates a pair of counter-rotating and symmetrical vortex flows when flowing in the tube, the vortex flows are called dean vortex flows, the laminar flows exert inertial lifting force on blood cells in the blood, the inertial lifting force stabilizes the blood cells at an equilibrium position in the cross section, and the dean vortex flows through the blood cells under the combined action of the inertial lifting force and the dean traction force. In this embodiment, by designing the size and length of the asymmetric curved tubular structure, the blood cells are balanced by force only on one side of the channel section under the condition of proper flow rate, the flow of the blood is single-beam focused flow, and the blood is separated into two layers of flowing liquids through the inertial separation channel 22, wherein the two layers of flowing liquids are blood cells and plasma respectively. Referring to fig. 1 together, under the shunting action of the asymmetric Y-shaped structure of the shunting channel 23, the plasma enters the plasma analyzing channel 24, and passes through the hepatitis a analyzing platform 241, the hepatitis b analyzing platform 242, the arthritis analyzing platform 243 and the myocardial infarction analyzing platform 244 in sequence for specific antibody detection; the blood cells are drained into the blood cell analysis channel 25, sequentially passing through the platelet counting platform 251, the red blood cell counting platform 252, the hemoglobin analysis platform 253, the white blood cell counting platform 254, and the blood type analysis platform 255. The plasma and blood cell waste after the analysis and detection are discharged through the waste liquid channel 26.
Fig. 3 is a schematic structural diagram of a preferred embodiment of a plasma analysis channel in a multi-channel microfluidic chip for rapid self-test of blood according to the present invention, as shown in fig. 3. In this embodiment, the hepatitis a analysis platform 241 includes an IgM detection unit 2411 and an IgA detection unit 2412, and the IgM detection unit 2411 and the IgA detection unit 2412 are integrated on the microfluidic chip substrate 1. The IgM probe unit 2411 is coated with an immune layer of hepatitis A Virus antigen (HAV-Ag) as an antigen for capturing specific anti-HAV-IgM in plasma to form an antigen-antibody complex, and the specific binding between the antigen and the antibody generates an electrochemical signal which is quantitatively detected, analyzed and conducted by the IgM probe unit 2411. The IgA detection unit 2412 is coated with an anti-human μ chain immune layer for capturing specific anti-HAV-IgA in plasma to form an antigen-antibody complex and generate an electrochemical signal, and the IgA detection unit 2412 is used for quantitatively detecting, analyzing and conducting the content of anti-HAV-IgA in the plasma. The hepatitis A analysis platform 241 analyzes the hepatitis A infection level by integrating the detection results of the IgM detection unit 2411 and the IgA detection unit 2412.
The hepatitis b analysis platform 242 includes a hepatitis b surface antigen (HBsAg) detection unit 2421, a hepatitis b surface antibody (HBsAb) detection unit 2422, a hepatitis b e antigen (HBeAg) detection unit 2423, a hepatitis b e antibody (HBeAb) detection unit 2424, and a hepatitis b core antibody (hbcabs) detection unit 2425. Electrochemical signals generated by antigen-antibody specific recognition and combination on the HBsAg detection unit 2421, the HBsAb detection unit 2422, the HBeAg detection unit 2423, the HBeAb detection unit 2424 and the HBcAb detection unit 2425 are respectively used for quantitatively detecting, analyzing and conducting the content of HBsAg, HBsAb, HBeAg, HBeAb and HBcAb in blood plasma. The myocardial infarction analysis platform 243 obtains five examination results of hepatitis b by synthesizing results of the HBsAg detection unit 2421, the HBsAb detection unit 2422, the HBeAg detection unit 2423, the HBeAb detection unit 2424 and the hbcabb detection unit 2425, so as to judge the hepatitis b infection condition.
The arthritis analysis platform 243 includes four Rheumatoid Factor (RF) detection units, which are an RF-IgM detection unit 2431, an RF-IgA detection unit 2432, an RF-IgG detection unit 2433, and an RF-IgE detection unit 2434. The RF-IgM detection unit 2431, the RF-IgA detection unit 2432, the RF-IgG detection unit 2433 and the RF-IgE detection unit 2434 are respectively used for specifically capturing IgM type, IgG type IgA type and IgE type rheumatoid factors in blood serum, and the content of each type rheumatoid factor in the blood plasma is analyzed through an electrochemical signal generated by forming an antigen-antibody complex. The first arthritis analysis platform 243 judges the rheumatoid arthritis by integrating the detection results of the RF-IgM detection unit 2431, the RF-IgA detection unit 2432, the RF-IgG detection unit 2433, and the RF-IgE detection unit 2434.
The myocardial infarction analysis platform 244 includes a hypersensitivity C-reactive protein (hs-CRP) detection unit 2441 and a creatine kinase isoenzyme (CK-MB) detection unit 2442. The hs-CRP detection unit 2441 and the CK-MB detection unit 2442 are used to specifically capture hs-CRP and CK-MB, respectively, which are recognized as serum markers for myocardial infarction with high specificity. The myocardial infarction analysis platform 244 can rapidly determine myocardial infarction by integrating the results of the hs-CRP detection unit 2441 and the CK-MB detection unit 2442.
Fig. 4 is a schematic structural diagram of a blood cell analysis channel in a multi-channel microfluidic chip for rapid self-test of blood according to a preferred embodiment of the present invention, as shown in fig. 4. In the present embodiment, the blood cell analyzing channel 25 includes the platelet counting platform 251, the red blood cell counting platform 252, the hemoglobin analyzing platform 253, the white blood cell counting platform 254 and the blood type analyzing platform 255. The platelet count platform 251, the red blood cell count platform 252, and the white blood cell count platform 253 sort different sizes of blood cells by using different sized tubes based on the volume difference between red blood cells, platelets, and white blood cells.
In this example, as shown in connection with fig. 4, the platelet counting platform 251 includes a platelet screen 2511 and a platelet counter 2512. The red blood cell counting platform 252 includes a red blood cell sieve 2521, a red blood cell drainage tube 2522, and a red blood cell counter 2523. The platelet screen 2511 comprises micro-parallel tubes (e.g., 5 μm in diameter) and the platelet counter 2512 is disposed below the platelet counting platform 251. The erythrocyte screen 2521 comprises a parallel screening pipe (for example, 10 μm in diameter), the erythrocyte counter 2522 is arranged at the bottom of the erythrocyte screen 2521, the erythrocyte drainage tube 2523 is arranged at the side of the erythrocyte screen 2521, the erythrocyte drainage tube 2523 is connected with the hemoglobin measuring platform 253, and the blood cell analysis channel 25 is connected with the leukocyte counting platform 254.
When blood cells are guided through the platelet counting platform 251 by the blood cell analyzing channel 25, platelets with the smallest volume enter the platelet sieve 2511 and sink to the bottom of the platelet sieve 2511, the sieved platelets are analyzed and metered by the platelet counter 2512 based on the electrical impedance principle, and the rest of the blood cells continue to pass through the red blood cell counting platform 252, wherein red blood cells with smaller volume are sieved by the red blood cell sieve 2521 and flow into the red blood cell drainage tube 2523, and the red blood cell drainage tube 2523 is only used for red blood cells to queue and pass through. The red blood cell counter 2522 is used when red blood cells pass through, but is not limited to, counting the number of red blood cells by the laser counting principle. The red blood cells enter the hemoglobin measurement platform 253.
As shown in fig. 5, fig. 5 is a schematic structural diagram of a hemoglobin measurement platform of a multi-channel microfluidic chip for rapid self-test of blood according to the present invention. In this embodiment, the hemoglobin measurement platform 253 includes a red blood cell lysis unit 2531, a hemoglobin test unit 2532, and a valve 2533. The red blood cell lysis unit 2531 and the hemoglobin test unit 2532 are both disc structures and are perpendicular to each other, which is beneficial to solution mixing. The valve 2533 is disposed between the red blood cell lysis unit 2531 and the hemoglobin test unit 253, and a hemolytic agent is disposed in the red blood cell lysis unit 2531. When the red blood cells enter the hemoglobin test unit 2532, the valve 2533 is opened, and the hemolytic agent flows into the hemoglobin test unit 2532, so that the red blood cells release hemoglobin, which is combined with the hemolytic agent to form a hemoglobin derivative. The hemoglobin test unit 2532 measures the concentration of the hemoglobin derivative by absorbance, and the waste liquid after the test is discharged from the waste liquid channel 26.
Referring again to FIG. 4, the leukocytes remaining in the blood cell analysis channel 25 continue to pass through the leukocyte counting platform 254, the leukocyte counting platform 254 being a micro-channel structure (e.g., 21 μm in diameter) through which only a single leukocyte line passes. The back of the white blood cell counting platform 254 is provided with a white blood cell counter 2541 for counting the number of white blood cells passing through the white blood cell counting platform 254 based on the laser counting principle. The white blood cells flow through the white blood cell counting platform 254 into the blood grouping analysis platform 255. The blood type analysis platform 255 comprises a cell lysis unit 2551 and a blood type analysis unit 2552, and leukocyte lysate is arranged in the cell lysis unit 2551. When the leukocyte flows into the cell lysis unit 2551, the leukocyte lysate is fully mixed with the leukocytes, the leukocytes are broken to form a mixed solution, the mixed solution flows into the blood type analysis unit 2552, the blood type analysis unit 2552 analyzes the genotype of the ABO blood type by using, but not limited to, a PCR technology, and the analyzed waste liquid is discharged through the waste liquid channel 26.
The multi-channel micro-fluidic chip for rapidly and automatically detecting blood can be repeatedly used, when the blood detection is finished, the sample injection channel 21 is connected with each pipeline inside the water pump for washing and is discharged by the waste liquid channel 26, and reagents such as hemolytic agent, leukocyte lysate and the like can be used after the reagents are used, but not limited to injection supplementation. In addition, each pipeline structure and pipeline junction in the multichannel microfluidic chip are designed by adopting a radian structure, so that liquid can flow conveniently. The multi-channel micro-fluidic chip realizes various blood detection functions at one time through a micro-fluidic chip structure integrated on a centimeter scale, is small in size and convenient to carry, and is convenient for family self-checking use.
The above description is only a preferred embodiment of the present invention, and not intended to limit the scope of the present invention, and all modifications of equivalent structures and equivalent functions made by the present specification and drawings, or directly or indirectly applied to other related technical fields, are included in the scope of the present invention.
Claims (7)
1. The utility model provides a multichannel micro-fluidic chip for quick self-checking blood, includes base and cover plate, its characterized in that, integrated sample channel, inertia separation channel, reposition of redundant personnel passageway, plasma analysis passageway and blood cell analysis passageway in the cover plate, sample channel, inertia separation channel and reposition of redundant personnel passageway link to each other in proper order, reposition of redundant personnel passageway adopts Y type structure to be connected to plasma analysis passageway with blood cell analysis passageway, wherein:
the sample introduction channel is used for inputting blood to be detected;
the inertial separation channel separates the blood into plasma and blood cells, which flow into the diversion channel;
the shunting channel introduces the plasma into the plasma analysis channel through the Y-shaped structure to perform detection analysis on the plasma, and introduces the blood cells into the blood cell analysis channel to perform detection analysis on the blood cells;
the blood cell analysis channel comprises a platelet counting platform, a red blood cell counting platform, a hemoglobin analysis platform, a white blood cell counting platform and a blood type analysis platform;
the platelet counting platform comprises a platelet sieve and a platelet counter;
the red blood cell counting platform comprises a red blood cell sieve, a red blood cell drainage tube and a red blood cell counter;
the platelet counting platform separates platelets in the hemocytes by controlling the size of the channel of the platelet sieve and counts the platelets by the platelet counter;
the red blood cell counting platform separates red blood cells in the blood cells by controlling the size of the pipeline of the red blood cell sieve, and counts the red blood cells by the red blood cell counter;
the leucocyte counting platform separates and counts leucocytes in the leucocytes by controlling the size of a pipeline of the leucocyte counting platform.
2. The multi-channel microfluidic chip for rapid self-test of blood according to claim 1, wherein a waste liquid channel is further integrated in the cover sheet, the plasma analysis channel and the blood cell analysis channel are both connected to the waste liquid channel, and the waste liquid channel is used for guiding out waste liquid generated by analyzed plasma and blood cells.
3. The multi-channel microfluidic chip for rapid self-test of blood according to claim 1, wherein the plasma analysis channel comprises a hepatitis A analysis platform, a hepatitis B analysis platform, an arthritis analysis platform and a myocardial infarction analysis platform.
4. The multi-channel microfluidic chip for rapid blood self-test according to claim 3, wherein:
the hepatitis A analysis platform comprises an IgM detection unit and an IgA detection unit;
the hepatitis B analysis platform comprises an HBsAg detection unit, an HBsAb detection unit, an HBeAg detection unit, an HBeAb detection unit and an HBcAb detection unit;
the arthritis analysis platform comprises an RF-IgM detection unit, an RF-IgA detection unit, an RF-IgG detection unit and an RF-IgE detection unit;
the myocardial infarction analysis platform comprises an hs-CRP detection unit and a CK-MB detection unit;
the immunoassay platform judges the detection result of the blood plasma by integrating the detection data of each detection unit in the immunoassay platform on the blood plasma.
5. The multi-channel microfluidic chip for rapid self-test of blood according to claim 1, wherein when blood cells flow through the blood cell analysis channel, the blood platelets are retained on the blood platelet counting platform, red blood cells pass through the red blood cell counting platform, enter the hemoglobin analysis platform through the red blood cell drainage tube for hemoglobin separation determination, and the remaining white blood cells are counted by the white blood cell counting platform and enter the blood type analysis platform for blood group genotype analysis.
6. The multi-channel microfluidic chip for rapid self-test of blood according to claim 1, wherein the hemoglobin analysis platform comprises a red blood cell lysis unit, a hemoglobin test unit and a valve, wherein:
the red blood cell lysis unit and the hemoglobin test unit are both disc structures and are arranged vertically;
the valve is arranged between the red blood cell lysis unit and the hemoglobin test unit;
the erythrocyte lysis unit is internally provided with a hemolytic agent.
7. The multi-channel micro-fluidic chip for rapid self-test of blood according to any one of claims 1 to 6, wherein the sample feeding channel, the inertial separation channel, the shunt channel, the plasma analysis channel, the blood cell analysis channel and the waste liquid channel all adopt a radian structure.
Priority Applications (2)
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| CN201610068754.XA CN105728069B (en) | 2016-01-30 | 2016-01-30 | Multi-channel micro-fluidic chip for rapidly self-checking blood |
| PCT/CN2016/101868 WO2017128768A1 (en) | 2016-01-30 | 2016-10-12 | Multi-channel microfluidic chip for fast self-testing of blood |
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| CN201610068754.XA CN105728069B (en) | 2016-01-30 | 2016-01-30 | Multi-channel micro-fluidic chip for rapidly self-checking blood |
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| CN105728069A (en) | 2016-07-06 |
| WO2017128768A1 (en) | 2017-08-03 |
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