CN105724392A - Application of pesticide composition containing JS399-19 and ipconazole to prevention and treatment of rice bakanae disease - Google Patents

Abstract

The invention relates to the use of a pesticide composition containing cyclostrobin and bacconazole in the prevention and treatment of rice bakanae disease. The invention discloses a new application of a pesticide composition for preventing and treating rice bakanae disease. It contains 1-60 parts by weight of cyclostrobin and 1-40 parts by weight of cloconazole. The pesticide composition of the present invention has a significant synergistic effect on inhibiting the mycelia growth of rice bakanae disease, and also has a good effect on field control of rice bakanae disease, and can significantly reduce the amount of pesticides, reduce costs, reduce environmental pollution and pesticide residues. The invention can effectively control the drug resistance of rice bakanae pathogens to the mainstream drugs carbendazim and prochloraz, reduce the drug resistance risk of cyclostrobin in agricultural production, increase its effective service life, and can also treat soil-borne sickle The diseases at the seedling stage caused by fungi and Rhizoctonia will have application value for the control of rice bakanae disease resistance in my country and increase social, economic and ecological benefits.

Description

A kind of pesticide composition containing cyclostrobin and paconazole is effective in preventing and treating rice bakanae disease Uses in

technical field

The invention belongs to the technical field of pesticides, and in particular relates to the use of a pesticide composition in preventing and treating bakanae disease of rice, and also relates to the use of an agricultural fungicide in preventing and treating bakanae disease of rice.

Background technique

Cyclostrobin (test number: JS399-19, English name: phenamacril), chemical name: ethyl 2-cyano-3-amino-3-phenylacrylate, molecular formula: C ₁₂ H ₁₂ N ₂ O ₂ , chemical The structural formula is as follows:

Cyanostystrobin is a new type of cyanoacrylate selective fungicide developed by the Jiangsu Base of the National Southern Pesticide Innovation Center. Specialized inhibition. The new fungicide has obtained the Chinese invention patent, and completed the provisional pesticide registration in 2005 and the official registration in 2011. Since cyanoxystrobin entered the market, it has played an important role in the prevention and control of wheat scab and rice bakanae disease. Especially in areas where wheat scab and rice bakanae are resistant to traditional fungicides such as carbendazim, it has become an effective alternative agent. It is very easy to produce drug resistance under the environment, and there is a high risk of drug resistance. Therefore, in order to delay or overcome the resistance of pathogenic bacteria to cyclostrobin and expand the spectrum of disease prevention, it is an important strategy to use cyclostrobin in rotation or in combination with fungicides with other modes of action.

Ipconazole (code: KNF-317, English name: Ipconazole), chemical name: 2-((4-chlorobenzyl)methyl-5-(1-isopropyl)-1-(1H-1,2 , 4-triazole-1-methyl) cyclopentanol, molecular formula: C ₁₈ H ₂₄ ClN ₃ O, chemical structural formula is as follows:

Conconazole is a triazole ergosterol biosynthesis inhibitor, which has systemic, protective and therapeutic activities, and can effectively prevent and control a variety of diseases caused by Ascomycetes, Basidiomycetes and Deuteromycetes. The conduction of stems and leaves has a good control effect on head blight, leaf spot and fusarium wilt.

Rice bakanae disease is a common seed-borne fungal disease, which can occur in all rice planting areas in the world. It is mainly transmitted by infected seeds. up to 50% or more. In recent years, especially in Jiangsu Province, the incidence rate has been increasing year by year, and the degree of occurrence is also increasing. The incidence rate of seriously diseased fields is as high as 35%. The production mainly uses carbendazim, prochloraz, etc. to soak seeds or spray for control. However, carbendazim is almost ineffective against bakanae disease due to resistance problems. Prochloraz has been used as a rice seed soaking agent to control bakanae disease for more than 20 years. Studies have shown that rice bakanae disease has a high risk of resistance to prochloraz, and some areas have produced moderately resistant and highly antibacterial strains, and the use of prochloraz to prevent and control bakanae disease has a high risk of drug resistance.

For this reason, on the basis of summarizing the prior art, the present inventors combined cyclostrobin and bacconazole with different mechanisms of action to prevent and control rice bakanae disease. Through a large number of experimental studies and analysis, the fungicide combination Seed treatment with plants at the seedling stage has a better synergistic effect on rice bakanae disease.

Contents of the invention

Purpose of the invention: the purpose of the invention is to provide a kind of application of the pesticide composition with synergistic and expanded antibacterial spectrum in the prevention and treatment of rice bakanae disease. Another object of the present invention is to provide an application of an agricultural fungicide preparation in the prevention and treatment of rice bakanae disease.

Technical solution: In order to solve the above-mentioned technical problems, the present invention provides the use of a pesticide composition containing cyclostrobin and inconazole in the prevention and treatment of rice bakanae disease. In the pesticide composition, the weight ratio of cyclostrobin to cloconazole is 1:60-40:1.

Wherein, the weight ratio of the above-mentioned cyclostrobin and pacloconazole is 1:30-20:1.

Wherein, the weight ratio of the above-mentioned cyclostrobin and pacloconazole is 1:15˜10:1.

The invention also provides an application of an agricultural fungicide preparation containing the pesticide composition in preventing and treating rice bakanae disease.

Wherein, the above-mentioned agricultural fungicide formulation also includes acceptable carriers and adjuvants in the pesticide formulation.

Wherein, the above-mentioned agricultural fungicide preparation is a wettable powder, a suspension, a microemulsion or a water-dispersible granule.

Wherein, the carrier described in the above-mentioned agricultural fungicide preparation is one or more of water, kaolin, diatomaceous earth, attapulgite or light calcium carbonate.

Among them, the auxiliary agents described in the above-mentioned agricultural fungicide preparations are ethanol, methanol, ethylene glycol, propylene glycol, NNO-1, NNO-7, xanthan gum, polyethylene glycol, glycerin, pull open powder, dodecane Sodium sulfate, sodium dodecylbenzenesulfonate, ammonium sulfate, alkylphenol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether, phenylethylphenol formaldehyde resin polyoxyethyl ether, alkylphenol polyoxyethylene Ether phosphate, fatty acid polyoxyethylene ester, sulfonic acid polyoxymethylene condensate, N-methylpyrrolidone, calcium alkylbenzenesulfonate, sodium butylnaphthalenesulfonate, benzoic acid, sodium lignosulfonate, carboxymethyl One or more of cellulose, silicone compounds, magnesium aluminum silicate or polyvinyl alcohol.

Beneficial effects: the inventor provides a theoretical and technical basis for the present invention through the research on the occurrence of plant diseases, the technology of using control agents, the mechanism of action and drug resistance. The present invention uses novel fungicides cyclostrobin and baconazole to combine control of rice bakanae disease, the purpose of which is to obtain a synergistic formula, reduce the amount of pesticides and production costs, improve the control effect, control drug resistance and expand the spectrum of disease prevention .

Indoor and field drug efficacy tests of the present invention show that the combination of cyclostrobin and clobutanil for preventing and treating rice bakanae disease is safe in use, remarkable in drug effect and environment-friendly. Compared with other pesticides, it has the following advantages:

1. In the pesticide composition for preventing and treating bakanae disease of rice provided by the present invention, cyclostrobin and clocloxanil are respectively fungicides with different mechanisms of action, which are different from the mainstream medicaments carbendazim and prochloraz used in the past to prevent and control rice bakanae disease. There is no cross-resistance to other agents.

2. The pesticide composition for preventing and treating rice bakanae provided by the present invention has the advantages of high efficiency, low toxicity, low residue, good quick-acting, long-lasting effect, and environmental friendliness.

3. The pesticide composition cyclostrobin and bacconazole provided by the present invention for preventing and controlling rice bakanae has compatibility, improves the fungicidal activity, reduces the dosage of a single fungicide, and saves the cost of using pesticides.

4. The pesticide composition for preventing and treating rice bakanae disease provided by the present invention can reduce the risk level of resistance of pathogenic bacteria to chemical agents, is conducive to maintaining the sensitivity of pathogenic bacteria, and can delay the occurrence of drug resistance of pathogenic bacteria to a single agent in the formula.

5. the pesticide composition of preventing and treating rice bakanae disease provided by the present invention can not only effectively prevent and treat rice bakanae disease, but also can control the seedling blight caused by soil-borne Fusarium and Rhizoctonia, reducing the damage of the disease to seedlings. Harm, improve seedling quality.

detailed description

Example 1: 20% cyclostrobin · cloconazole wettable powder

Cyclostrobin 4%, Paconazole 16%, NNO-1 1%, Pull open powder 3%, Sodium dodecylbenzene sulfonate 2%, Attapulgite 30%, The rest is supplemented by light calcium carbonate to 100%. The above components were thoroughly mixed, pulverized by a sand mill and sieved through a 300-mesh sieve to obtain a 20% cyclostrobin-conconazole wettable powder.

Example 2: 60% cyclostrobin · cloconazole wettable powder

Cyclostrobin 40%, Paconazole 20%, NNO-1 1%, Pull open powder 3%, Sodium dodecylbenzene sulfonate 2%, Attapulgite 30%, The rest is supplemented by light calcium carbonate to 100%. The above components were thoroughly mixed, pulverized by a sand mill and sieved through a 300-mesh sieve to obtain a 60% cyclostrobin-conconazole wettable powder.

Example 3: 40% cyclostrobin·canconazole wettable powder

Cyclostrobin 20%, Paconazole 20%, NNO-1 1%, Pull open powder 3%, Sodium dodecylbenzene sulfonate 2%, Attapulgite 30%, The rest is supplemented by light calcium carbonate to 100%. The above components were thoroughly mixed, pulverized by a sand mill and passed through a 300-mesh sieve to obtain a 40% cyclostrobin-conconazole wettable powder.

Example 4: 18% cyclostrobin · cloconazole suspension concentrate

Cyclostrobin 4%, Buconazole 14%, Ethylene Glycol 4%, Propylene Glycol 4%, NNO-1 1%, NNO-7 1% Dispersion Agent, Xanthan Adhesive 0.5%, Polyethylene Glycol 1%, the rest is supplemented with water to 100%, the above components are fully mixed, and pulverized by a sand mill until 90% of the agent particle diameter is less than or equal to 5 μm, and 18% cyclostrobin-conconazole suspension concentrate is obtained.

Example 5: 42% cyclostrobin · cloconazole suspension concentrate

Cyclostrobin 30%, Buconazole 12%, Ethylene Glycol 4%, Propylene Glycol 4%, NNO-1 1%, NNO-7 1% Dispersion Agent, Xanthan Adhesive 0.5%, Polyethylene Glycol 1%, and the rest is water to make up to 100%. The above components are fully mixed, and pulverized by a sand mill until 90% of the agent particle diameter is less than 5 μm, to obtain 42% cyclostrobin-conconazole suspension concentrate.

Example 6: 22% cyclostrobin · cloconazole suspension concentrate

Cyclostrobin 20%, Buconazole 2%, Ethylene Glycol 4%, Propylene Glycol 4%, NNO-1 1%, NNO-7 1% Dispersion Agent, Xanthan Adhesive 0.5%, Polyethylene Glycol 1%, and the rest is water to make up to 100%. The above components are fully mixed, and pulverized by a sand mill until the diameter of 90% of the drug particles is less than or equal to 5 μm to obtain a 22% cyclostrobin-conconazole suspension.

Example 7: 23.25% cyclostrobin·canconazole suspension concentrate

Cyclostrobin 22.5%, Buconazole 0.75%, Ethylene Glycol 4%, Propylene Glycol 4%, NNO-1 1%, NNO-7 1% Dispersion Agent, Xanthan Gum Adhesive 0.5%, Polyethylene Glycol 1%, the rest is supplemented with water to 100%, fully mix the above components, and grind them through a sand mill until the diameter of 90% of the drug particles is ≤ 5 μm, to obtain 23.25% cyclostrobin-conconazole suspension concentrate

Example 8: 18% cyclostrobin · cloconazole microfloating agent

Cyclostrobin 10%, Buconazole 8%, N-Methylpyrrolidone 1%, Calcium Alkylbenzene Sulfonate 2%, Phenylphenol Formaldehyde Resin Polyoxyethyl Ether 2%, Ethylene Glycol 1.5% , 0.8% of silicone compounds, 1% of xanthan gum, 1% of magnesium aluminum silicate, and the rest is supplemented to 100% by water.

Add cyclostrobin, cloconazole, solvent N-methylpyrrolidone, emulsifier calcium alkylbenzene sulfonate and phenethylphenol formaldehyde resin polyoxyethyl ether in the above ratio, and dissolve into a homogeneous oil phase ; Mix water, antifreeze ethylene glycol, thickener xanthan gum, and defoamer silicone compounds to form a uniform water phase. Under high-speed stirring, the water phase is added to the oil phase to prepare 18% cyclostrobin·sconazole microfloating agent.

Example 9: 24% cyclostrobin · cloconazole water dispersible granules

Cyclostrobin 4%, Buconazole 20%, Ammonium Sulfate 10%, Alkylphenol Polyoxyethylene Ether 2.5%, Sodium Dodecyl Benzene Sulfonate 2.5%, Fatty Acid Polyoxyethylene Ester 3.5%, and the rest are light Calcium carbonate supplemented to 100%, the above components are fully mixed, the mother powder is prepared by crushing, the mother powder is mixed with an appropriate amount of aqueous solution, high-speed shearing and sand mill grinding, and then fluidized bed granulation, drying, After sieving, 24% cyclostrobin·conconazole water-dispersible granules can be prepared.

Example 10: 32% cyclostrobin·canconazole water dispersible granules

Cyclostrobin 24%, Buconazole 8%, Ammonium Sulfate 10%, Alkylphenol Polyoxyethylene Ether 2.5%, Sodium Dodecyl Benzene Sulfonate 2.5%, Fatty Acid Polyoxyethylene Ester 3.5%, and the rest are light Calcium carbonate supplemented to 100%, the above components are fully mixed, the mother powder is prepared by crushing, the mother powder is mixed with an appropriate amount of aqueous solution, high-speed shearing and sand mill grinding, and then fluidized bed granulation, drying, After sieving, 32% cyclostrobin·conconazole water-dispersible granules can be prepared.

Experimental Example: Indoor Activity Determination

The toxicity and interaction synergistic coefficient of the composition of different ratios of cyclostrobin and penconazole to the target were determined indoors. The method is to dissolve the original drug of cyclostrobin and pacloconazole with methanol to form a mother solution of 1000 μg/mL, and store it at 4°C for later use. According to the effective content, cyclostrobin and pacloconazole were prepared respectively as 1:40, 1:20, 1:10, 1:5, 1:1, 5:1, 10:1, 20:1, 40:1 A series of test solutions of the mixed agent were then mixed into the culture medium (PDA) to measure the inhibitory activity on the mycelia growth of Bakanae oryzae, and to calculate the EC ₅₀ value and the synergistic coefficient.

The average inhibition rate of hyphae growth=[(average value of control colony diameter-average value of treated colony diameter)/(average value of control colony diameter-diameter of inoculated bacterial cake)]×100%. DPS data processing system was used to calculate the regression equation and EC ₅₀ value. Calculate the synergistic factor (SR) according to Wadley method.

According to the synergistic coefficient (SR) to evaluate the type of joint effect of drug mixture, that is, SR<0.5 is an antagonistic effect, 0.5≤SR≤1.5 is an additive effect, and SR>1.5 is a synergistic effect. SR= _EC50 (Eth)/ _EC50 (Eob), _EC50 (Eth)=(a+b)/[(a/ _EC50A )+(b/ _EC50B )]. Among them, A and B are single agents of fungicides respectively, a and b are the proportions of corresponding single agents in the mixture, EC ₅₀ (Eth) is the theoretical value of EC ₅₀ of the mixture, and EC ₅₀ (Eob) is the measured EC ₅₀ of the mixture value.

According to the results in Table 1, it can be seen that when the proportioning ratio of cyclostrobin and pacloconazole is 1-60:40-1, the _EC50 against rice bakanae pathogen is 0.0377-0.2443 μg/ml, and the synergistic coefficient is 1.66～2.10, all achieved the synergistic effect.

Table 1: Screening of indoor formulations of cyclostrobin and penconazole against rice bakanae pathogen

Application example 1: field efficacy test of cyclostrobin and cloconazole combination for soaking seeds to prevent and control rice bakanae disease

Using the fungicide preparations of Examples 1 to 10 and the single agents of the control agents cyclostrobin, cloconazole and prochloraz, the medicaments of the different preparations of Examples 1 to 10 were respectively diluted to 3000 to 10000 times the drug solution for soaking seeds, wherein The single agent of cyclostrobin is 25% suspension concentrate produced by Jiangsu Pesticide Research Institute; Fresh amine cream. The rice to be tested was Zhendao 16, soaked at 25°C for 48 hours, and then germinated until it was white and sowed on seedling trays. The seedling raising method is dry seedling raising, and the seedlings are transplanted to the field after 25 days of age. The test site is located in the net room of the Plant Protection College of Nanjing Agricultural University. The rate of diseased plants of bakanae is investigated and the control effect is calculated at the seedling stage and heading stage before transplanting. The results are shown in the table 2.

Table 2: Field efficacy test of the combination of cyclostrobin and penconazole for soaking seeds to control rice bakanae disease

Application example 2: Field drug efficacy test of cyclostrobin and buclobutanil combined seed coating to control rice bakanae disease

Take several grams of the fungicide preparations of Examples 1-10 and the single agent of cyclostrobin, clocloxanil and prochloraz respectively, dilute with 800 milliliters of drug solution per 10 kilograms of seeds and stir with rice seeds, and stir evenly Leave to dry. Among them, the single agent of cyclostrobin is a 25% suspension concentrate produced by Jiangsu Pesticide Research Institute, the single agent of cloconazole is a 12% suspension concentrate processed in the laboratory, and the single agent of prochloraz is produced by Jiangsu Huifeng Agricultural Chemical Co., Ltd. 25% prochloraz EC. The tested rice was Zhendao 16. The coated rice seeds were sown on seedling trays. The cultivation and management method was dry seedling raising. The seedlings were transplanted in the field after 25 days. The diseased plant rate of seedlings was calculated and the control effect was calculated. The results are shown in Table 3.

Table 3: Field efficacy test of the combination of cyclostrobin and buconazole as a seed coating treatment for the control of rice bakanae disease

The test results of the composition soaking and coating treatment show that the preparations of the compositions of Examples 1 to 10 of the present invention have excellent control effects on the rice seed-borne disease bakanae disease, and the control effects are all above 90%, which is significantly higher than that of the control The control effect of the agent prochloraz.

Claims (7)

1. A use of a pesticide composition containing cyclostrobin and paconazole in the prevention and treatment of rice bakanae disease, characterized in that the weight ratio of cyclostrobin and paconazole is 1:60～40:1. 2. The use according to claim 1, characterized in that the weight ratio of cyclostrobin to cloconazole is 1:30 to 20:1. 3. The use according to claim 1, characterized in that the weight ratio of cyclostrobin and cloconazole is 1:15-10:1. 4. an agricultural bactericide preparation containing the described pesticide composition of claim 1-3 is used in the prevention and treatment of rice bakanae disease, and it is characterized in that described agricultural bactericide preparation also comprises acceptable carrier in pesticide and Auxiliary. 5. The use according to claim 4, characterized in that the agricultural fungicide is a wettable powder, a suspension, a microemulsion or a water-dispersible granule. 6. The use according to claim 4, characterized in that the carrier is one or more of water, kaolin, diatomaceous earth, attapulgite or light calcium carbonate. 7. purposes according to claim 4, it is characterized in that described auxiliary agent is ethanol, methyl alcohol, ethylene glycol, propylene glycol, NNO-1, NNO-7, xanthan gum, polyethylene glycol, glycerol, propylene glycol Open powder, sodium lauryl sulfate, sodium dodecylbenzenesulfonate, ammonium sulfate, alkylphenol polyoxyethylene ether, lauryl alcohol polyoxyethylene ether, phenylethylphenol formaldehyde resin polyoxyethyl ether, Alkylphenol polyoxyethylene ether phosphate, fatty acid polyoxyethylene ester, polyoxymethylene sulfonate condensate, N-methylpyrrolidone, calcium alkylbenzenesulfonate, sodium butylnaphthalenesulfonate, benzoic acid, lignosulfonate One or more of sodium sodium phosphate, carboxymethyl cellulose, silicone compounds, magnesium aluminum silicate or polyvinyl alcohol.