CN105707126A - Bactericidal composition, preparation and application of bactericidal composition - Google Patents

Abstract

The invention provides a bactericidal composition, preparation and application thereof. The bactericidal active ingredient of the bactericidal composition is mainly composed of Bacillus amyloliquefaciens and antibiotics in a weight ratio of 10:0.5-20, and the antibiotics include kasugamycin, poly Any one of antimycin and Zhongshengmycin, the number of viable spores of Bacillus amyloliquefaciens is 5 to 50 billion/g. Bacillus amyloliquefaciens and the above-mentioned antibiotics are compounded to play a synergistic effect, which is better than a single chemical drug in preventing and controlling diseases.

Description

A kind of bactericidal composition, preparation and application thereof

technical field

The invention relates to the field of pesticides, in particular to a bactericidal composition, preparation and application thereof.

Background technique

With the rapid development of the national economy and the improvement of people's living standards, people have put forward higher and higher requirements for the safety of fruits and vegetables, food, and medicinal plant products. At present, in the production of fruits, vegetables, grains, and medicinal plants, the prevention and control measures are single, and the prevention and control of diseases mainly depends on chemical pesticides. Variety. Long-term use of a single dose of chemical pesticides is likely to induce serious disease resistance, resulting in a large increase in the amount of pesticides used, and the problem of residues is also aggravated. The control effect of chemical agents has gradually declined, and it has also caused serious pollution of the ecological environment and water quality, increasing the harm to human health. How to improve the control effect of pesticides on fruits, vegetables, grains, and medicinal plant diseases and reduce the use of pesticides has become the focus of attention of governments at all levels, scholars and farmers.

Contents of the invention

The object of the present invention is to provide a bactericidal composition, preparation and application thereof. The bactericidal composition has a good control effect on crop diseases, and has a long-term control effect, which can reduce the frequency of pesticide application, thereby reducing the occurrence of pesticides. A lot of residue. The combination of two fungicidal active ingredients can reduce the increase of crop resistance.

In order to realize the above-mentioned purpose of the present invention, special adopt following technical scheme:

A bactericidal composition, the bactericidal active ingredient of the bactericidal composition is mainly composed of Bacillus amyloliquefaciens and antibiotics in a weight ratio of 10:0.5 to 20; the antibiotics include kasugamycin, polyoxin and mesophytic bacteria Any one of the factors; the number of viable spores of the Bacillus amyloliquefaciens is 5 to 50 billion/gram.

Bacillus amyloliquefaciens produces a series of metabolites during its own growth process, and these metabolites have extensive and highly effective inhibitory activities against fungi and bacteria, thereby preventing and controlling crop diseases.

Antibiotics are a class of metabolites with anti-pathogen activity produced by microorganisms, higher animals and plants in the course of life, which can interfere with the development and function of other living cells. Among them, kasugamycin is a metabolite of actinomycetes, which has therapeutic and preventive effects on various bacterial and fungal diseases, and is safer for humans and animals. Polyoxin is a metabolite of Streptomyces aureus, which has broad-spectrum antibacterial effect, good compatibility with nature, and fast degradation. Zhongshengmycin is a metabolite of Streptomyces lavender genus Hainan variety, which has the characteristics of a broad bactericidal spectrum, and also has the effects of contact and penetration.

After the active ingredients of different fungicides are mixed, due to the differences in the composition, structure, and physical and chemical properties of each component, the commonality between the components generally manifests as a synergistic effect. Synergy refers to the synergistic interaction between different substances to produce a positive effect greater than that of the individual components. In addition, due to the use of biological pesticides, the application of chemical pesticides that are not easy to degrade and are highly toxic can be reduced, and the harm to the human body and the environment can be reduced.

The bactericidal composition provided by the present invention is a combination of Bacillus amyloliquefaciens and one of kasugamycin, polyoxin and Zhongshengmycin, and the two substances have synergistic effects on crop diseases through mutual synergy and prolong the effect of control time, especially for rice blast, ginseng root rot, apple leaf spot, apple tree rot, cucumber bacterial angular spot, rice white stripe leaf blight, citrus canker, apple moldy heart and Potato late blight has a good control effect.

The field drug efficacy test of rice blast shows that after 10 days of application, the fungicidal composition provided by the invention has a control effect of more than 77%, while a single agent has a control effect of less than 70%. The field efficacy test of ginseng root rot shows that 10 days after the second application, the fungicidal composition provided by the invention has a control effect of more than 72%, while a single agent has a control effect of less than 72%. The field efficacy test of apple tree spot defoliation showed that after 20 days of application. The field drug efficacy test of apple tree rot shows that the fungicidal composition provided by the invention has a control effect of more than 75%, while a single agent has a control effect of less than 74%. The field drug efficacy test of cucumber bacterial angular spot shows that after 15 days of application, the fungicidal composition provided by the invention has a control effect of more than 80%, while a single agent only has a control effect of less than 75%. The field drug efficacy test of rice bacterial blight shows that after 15 days of application, the fungicidal composition provided by the invention has a control effect of more than 80%, while a single dose has a control effect of less than 66%. The field drug efficacy test of citrus canker shows that after 14 days of application, the fungicidal composition provided by the invention has a control effect of more than 80%, while a single dose has a control effect of less than 77%. Field tests on apple moldy core show that the fungicidal composition provided by the invention has a control effect of more than 90%, while a single agent has a control effect of less than 84%. A field test on potato late blight shows that after 14 days of application, the fungicidal composition provided by the invention has a control effect of more than 69%, while a single agent has a control effect of less than 63%.

Preferably, the ratio of the weight of the Bacillus amyloliquefaciens to the weight of the antibiotic is 10:3.5-7, and the number of viable spores of the Bacillus amyloliquefaciens is 5-40 billion/g.

Preferably, the bactericidal active substance of the bactericidal composition comprises Bacillus amyloliquefaciens and kasugamycin, the ratio of the weight of the Bacillus amyloliquefaciens to the weight of the kasugamycin is 10:2～10, and the weight of the Bacillus amyloliquefaciens The number of live spores of Bacillus is 500-400 billion/g.

Preferably, the bactericidal active substance of the bactericidal composition comprises Bacillus amyloliquefaciens and polyoxin, the ratio of the weight of the Bacillus amyloliquefaciens to the weight of the polyoxin is 10:1.5-20, and the solution The number of viable spores of Bacillus amylobacter is 500 million to 30 billion per gram.

Preferably, the bactericidal active substance of the bactericidal composition comprises Bacillus amyloliquefaciens and Zhongshengmycin, the ratio of the weight of the Bacillus amyloliquefaciens to the weight of Zhongshengmycin is 10:0.5-4, and the solution The number of viable spores of Bacillus amylobacter is 5 billion to 20 billion per gram.

According to the disease characteristics of crop diseases, that is, the pathogenic types, appropriate antibiotics are selected. Polyoxin is mostly used against fungal diseases caused by fungi; Zhongshengmycin is mostly used against bacterial diseases caused by bacteria; kasugamycin has a wide bactericidal spectrum and has control effects on crop diseases caused by bacteria and fungi.

In addition, when choosing a drug, the degradation rate of the bactericidal combination should also be considered. When the drug is not completely degraded, it will lead to the problem of drug residue in the plant, which will affect the product quality of the crops. Therefore, when preventing and controlling crop diseases, it is necessary to select different antibiotics and Bacillus amyloliquefaciens according to the disease control cycle and the degradation rate of the drug. Cooperate in order to achieve good control effect.

The degradation rate of polyoxin and Bacillus amyloliquefaciens in plants was faster than that of kasugamycin and Zhongshengmycin. Therefore, when the time between the drug application time of crops and the harvest time of the crops is relatively short, it is advisable to select antibiotics with fast degradation speeds compounded with Bacillus amyloliquefaciens; According to the compounding of antibiotics with better control effect on diseases and Bacillus amyloliquefaciens.

A bactericidal preparation, comprising the above-mentioned bactericidal composition, the amount of the bactericidal composition accounts for 10.5-30% of the weight of the bactericidal preparation, and the balance is pesticide acceptable auxiliary materials. Excipients include solvents, dispersants, emulsifiers, stabilizers, antifreeze agents, thickeners, etc. and other known substances that are beneficial to the stability of active ingredients in the preparation and the exertion of their medicinal effects.

Preferably, the auxiliary material includes a UV protecting agent, and the amount of the UV protecting agent accounts for 0.1 to 15% by weight of the bactericidal preparation. Preferably, the UV protecting agent includes carboxymethyl cellulose, urea, starch, paste One or more of essence, Congo red, ascorbic acid, xanthan gum, and pure milk.

When Bacillus amyloliquefaciens and antibiotics in bactericidal preparations are over-irradiated by ultraviolet rays, problems such as protein deformation and cell membrane structure changes are prone to occur, thereby affecting their normal functions and reducing their control effect on crop diseases. Due to the weak penetrating ability of ultraviolet rays, the ultraviolet protective agent mixed with various excipients, Bacillus amyloliquefaciens and antibiotics can isolate ultraviolet sensitive substances from ultraviolet rays and increase the use time of bactericidal preparations.

The bactericidal preparation can be made into various dosage forms, such as wettable powder, aqueous solution, suspension concentrate, water-dispersible granules or granules, etc. Those skilled in the art can use auxiliary materials to make the bactericidal composition into corresponding dosage forms.

Wettable powder is more suitable for the prevention and treatment of rice blast, ginseng root rot, apple leaf spot, and apple rot.

The recommended application method is as follows: when controlling rice leaf blast, use 100g of wettable powder mixed with 50 kg of water per acre, and spray it at the early stage of the disease; when controlling rice panicle blast, spray it at the full earing stage of rice. When preventing and treating ginseng root rot, the wettable powder is diluted with water to 500 times liquid, and the roots are irrigated when the ginseng is diseased, and the roots are irrigated 0.5L per plant. When preventing and treating apple tree spot defoliation, the wettable powder is diluted 1000 times, and sprayed in the spring and autumn shoots of apple trees. When preventing and treating apple tree rot, the wettable powder is diluted 100 times, and then mixed with starch and 3% naphthaleneacetic acid solution to prepare a paste. In the diseased part of the apple tree, scrape off the rotten material, and then spread the paste evenly on the diseased part.

The water agent is more suitable for the control of bacterial angular spot of cucumber and bacterial blight of rice. The recommended application method is as follows: when controlling bacterial angular spot of cucumber, dilute the water agent 1000 times with water, and spray it at the early stage of the disease; when controlling rice bacterial blight, apply a mixture of 50 grams of water agent and 45 kg of water per mu The resulting mixture was sprayed at the early stage of the disease.

Water-dispersible granules are more suitable for the prevention and treatment of citrus canker and apple mold core. The recommended application method is as follows: when preventing and treating citrus canker, water-dispersible granules should be diluted 1000 times with water and sprayed at the early stage of the disease; when preventing and treating apple moldy heart disease, water-dispersible granules should be diluted 1000 times with water and sprayed in the full flowering period and flowering period of apple trees. Spray at the end.

Granules are more suitable for the prevention and control of potato late blight and potato late blight. The recommended method of use is as follows: When controlling potato late blight, mix the granules with soil to form poisonous soil, and then apply the poisonous soil directly to the soil at the root of the potato.

Preferably, when the bactericidal preparation is a wettable powder, by weight, it includes 12-30% of the bactericidal composition, 0.5-3% of an ultraviolet protective agent, 3-15% of a wetting agent, 2-15% of a dispersant, Nutrient 3-15%, the balance is filler.

Preferably, when the bactericidal preparation is an aqueous solution, by weight, it includes 10.5-14% of the bactericidal composition, 0.3-0.5% of an ultraviolet protective agent, 3-6% of a wetting agent, 5-20% of a dispersant, and an antifreeze 1-5% of agent, 0.1-2% of thickener, 0.1-3% of defoamer, 1-15% of nutrient, and the balance is water;

Preferably, when the bactericidal preparation is a water-dispersible granule, by weight, it includes 11.5-25% of the bactericidal composition, 0.5-1% of an ultraviolet protective agent, 3-10% of a dispersant, and 1-10% of a wetting agent , disintegrant 1-5%, nutritional agent 1-15%, the rest is filler;

Preferably, when the bactericidal preparation is a granule, by weight, it includes 11.5-15% of the bactericidal composition, 0.5-1% of an ultraviolet protective agent, 1-10% of the auxiliary materials including a dispersant, and a thickener. 01-5%, defoamer 0.1-5%, nutrient 1-15%, and the rest is castor oil;

Preferably, when the bactericidal preparation is a suspension, by weight, it includes 10-20% of the bactericidal composition, 0.1-2% of UV protector, 5-20% of dispersant, 1-5% of antifreeze, 0.1-2% thickening agent, 0.1-3% defoamer, 1-15% nutritional agent, and the balance is water.

Preferably, when the bactericidal preparation is a wettable powder, the wetting agent includes one or more of Sapindus powder, silkworm excrement, sodium dodecylbenzenesulfonate, and washing powder, and the dispersant includes One or more of lignin, lignosulfonate, alkylsulfonate, and the nutrient base includes one or more of glucose, lactose, sucrose, potassium fulvate, sodium fulvate, ammonium sulfate Various. Wettable powders are characterized by rapid dispersion in water and are therefore ideal for spray application. In addition, it also has the advantages of low production cost, convenient transportation, storage and use.

Preferably, when the bactericidal preparation is an aqueous solution, the wetting agent includes calcium dodecylbenzenesulfonate and/or pull-off powder BX, and the dispersant includes lignosulfonate, alkylphenol polyoxyethylene One or more of ether methyl ether condensate sulfate, alkyl sulfonate calcium salt, naphthalene sulfonic acid formaldehyde condensate sodium salt, glycerin fatty acid ester polyoxyethylene ether, and the antifreezing agent includes ethylene glycol, One or more of propylene glycol and glycerol, the defoamer includes one or more of silicone oil, silicone compounds, C10-20 saturated fatty acid compounds, C8-10 fatty alcohols, the nutrient Including one or more of glucose, lactose, sucrose, potassium fulvate, sodium fulvate, and ammonium sulfate. The water agent can be directly mixed with water for application. Since the water agent is thoroughly dispersed in water, it is very suitable for spraying. In addition, the manufacturing process of the water agent is simple, which can reduce its use cost.

Preferably, when the bactericidal preparation is a water-dispersible granule, the dispersant includes lignosulfonate, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, alkylsulfonate calcium salt, naphthalenesulfonic acid One or more of formaldehyde condensate sodium salt, alkylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester, fatty amine polyoxyethylene ether, glycerin fatty acid ester polyoxyethylene ether, and the wetting agent includes twelve One or more of sodium alkylsulfate, calcium dodecylbenzenesulfonate, pull open powder BX, saponin powder, silkworm excrement, sapindus chinensis powder, and the nutritional agent is glucose, lactose, sucrose, yellow One or more of potassium humate, sodium fulvic acid, and ammonium sulfate, and the disintegrating agent includes one or more of bentonite, ammonium sulfate, aluminum chloride, citric acid, succinic acid, and sodium bicarbonate Various. Water-dispersible granules can disintegrate and disperse quickly in water, so the application is convenient and quick.

Preferably, when the bactericidal preparation is a granule, the dispersant includes polycarboxylate, lignosulfonate, calcium salt of alkylsulfonate, sodium salt of naphthalenesulfonic acid formaldehyde condensate, alkylphenol polyoxygen One or more of vinyl ether, fatty acid polyoxyethylene ester, fatty amine polyoxyethylene ether, glycerin fatty acid ester polyoxyethylene ether, the wetting agent includes sodium lauryl sulfate, dodecylbenzenesulfonate Calcium acid calcium, pull open powder BX, saponin powder, silkworm excrement, sapindus powder in one or more, described thickening agent comprises carboxymethyl cellulose, carboxyethyl cellulose, methyl cellulose , magnesium aluminum silicate, one or more of polyvinyl alcohol, and the defoamer includes one or more of silicone oil, silicone compounds, C10-20 saturated fatty acid compounds, and C8-10 fatty alcohols. The nutritional agent is one or more of glucose, lactose, sucrose, potassium fulvate, sodium fulvate, and ammonium sulfate. Granules have a high density and are not easy to generate dust, so they do not pollute the environment and avoid absorption by the human body. When spraying, the directionality is high, and the spraying is more precise.

Preferably, when the bactericidal preparation is a suspension, the dispersant is one or more of polycarboxylate, alkylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester, fatty amine polyoxyethylene ether, Described antifreezing agent is one or more in ethylene glycol, propylene glycol, glycerol, and described thickening agent comprises carboxymethyl cellulose, carboxyethyl cellulose, methyl cellulose, magnesium aluminum silicate, One or more of polyvinyl alcohol, the defoamer is one or more of silicone oil, silicone compounds, C10-20 saturated fatty acid compounds, C8-10 fatty alcohol, and the nutrient is glucose, One or more of lactose, sucrose, potassium fulvate, sodium fulvate, and ammonium sulfate. The liquid suspending agent has high drug loading and high bactericidal activity.

Beneficial effects of the present invention:

1) Synergistic effect: In the compound bactericidal composition, the two bactericidal active ingredients act synergistically, and the bactericidal effect is higher than that of a single agent. 2) Reduction of dosage: due to the improvement of the efficacy, the dosage and frequency of the bactericidal active ingredients are reduced, thereby reducing the dosage and further reducing the pollution to the environment. 3) Reduce the resistance of crops: the use of two bactericidal active ingredients can increase the selection pressure of bacteria on drugs, thereby avoiding or delaying the emergence of drug resistance of bacteria. 4) Widening of bactericidal spectrum: there are differences in the bactericidal spectrum of the two bactericidal active ingredients, therefore, the bactericidal composition can have the effect of treating multiple diseases with one medicine. 5) Medication safety: Since the two bactericidal active ingredients in the invention belong to biological pesticides, they are easier to be decomposed and absorbed than chemical pesticides, thereby reducing drug residues. 6) Stimulate the growth of crops, increase the yield and income of crops, fruits and vegetables.

detailed description

Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only for illustrating the present invention, and should not be considered as limiting the scope of the present invention. Those who do not indicate the specific conditions in the examples are carried out according to the conventional conditions or the conditions suggested by the manufacturer. The reagents or instruments used were not indicated by the manufacturer, and they were all conventional products that could be purchased from the market.

Example 1

The present embodiment provides a bactericidal preparation whose dosage form is a wettable powder, and its composition is as follows:

2g kasugamycin, 10 billion live spores/gram of Bacillus amyloliquefaciens 10g, 6g sodium lauryl sulfate, 4g sodium lignosulfonate, 3g glucose, 1g ascorbic acid, kaolin supplement 100g, and make 2% kasugamycin ·10 billion live spores/gram Bacillus amyloliquefaciens wettable powder.

The wettable powder is produced by the following method: weighing each raw material according to the above weight, then mixing each raw material, pulverizing and stirring evenly to obtain the product.

Example 2

The present embodiment provides a bactericidal preparation whose dosage form is a wettable powder, and its composition is as follows:

5g kasugamycin, 5 billion live spores/gram Bacillus amyloliquefaciens 10g, 8g Sapinberry powder, 5g sodium lignosulfonate, 5g glucose, 1% ascorbic acid, kaolin to make up 100g to make 5% kasugamycin 50 Billion live spores/gram Bacillus amyloliquefaciens wettable powder.

Example 3

The present embodiment provides a bactericidal preparation whose dosage form is a wettable powder, and its composition is as follows:

7g kasugamycin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 8g Sapindus sativa powder, 5g sodium lignosulfonate, 3g glucose, 0.5g urea, and kaolin supplement 100g to prepare 7% kasugamycin 200 Billion live spores/gram Bacillus amyloliquefaciens wettable powder.

Example 4

The present embodiment provides a bactericidal preparation whose dosage form is a wettable powder, and its composition is as follows:

10g kasugamycin, 1 billion live spores/gram Bacillus amyloliquefaciens 10g, 4g sodium lauryl sulfate, 3g sodium lignosulfonate, 3g glucose, 1g ascorbic acid, kaolin make up 100g, make 10% kasugamycin. 1 billion live spores/gram Bacillus amyloliquefaciens wettable powder.

Example 5

The present embodiment provides a bactericidal preparation whose dosage form is a wettable powder, and its composition is as follows:

2g kasugamycin, 40 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g sodium lauryl sulfate, 5g sodium lignosulfonate, 15g glucose, 8g urea, kaolin make up 100g to make 2% kasugamycin. 40 billion live spores/gram Bacillus amyloliquefaciens wettable powder.

The bactericidal preparations in Examples 2 to 5 were all made according to the preparation method in Example 1.

Test example 1

Embodiments 1 to 5, 6% kasugamycin wettable powder, 1,000,000,000 live spores/gram of Bacillus amyloliquefaciens and a blank control are totally 8 treatments, each treatment is repeated 3 times, and the repetition area is 30 square meters, in rice leaf blast In the early stage of the disease, 30 kilograms of water per mu was used for uniform spraying, and the disease index of rice leaf blast in each treatment was investigated 10 days after the application, and the relative control effect was calculated based on the disease index of the control area. Before 10% of the rice was broken, the pesticide was applied at the full heading stage, and the investigation results of the rice panicle blast disease index of each treatment were investigated 10 days after the pesticide was applied, as shown in Table 1.

investigation method

Leaf blast survey method: Sampling at five diagonal points in each plot, surveying 5 rice clusters in each plot, surveying all leaves of 25 paddy clusters in each plot, recording the total number of leaves under investigation, the number of diseased leaves and the degree of disease.

Leaf grading standard for rice leaf blast:

Grade 0: no disease spots on leaves; Grade 1: less than 5 disease spots on leaves and less than 1 cm in length;

Grade 3: 6-10 lesions on leaves, some of which are longer than 1 cm;

Grade 5: 11-25 lesions on leaves, some lesions are connected into sheets, accounting for 10-25% of the leaf area;

Level 7: There are more than 26 diseased spots on the leaves, and the diseased spots are connected into sheets, accounting for 26-50% of the leaf area;

Grade 9: The diseased spots on the leaves are contiguous, accounting for more than 50% of the leaf area, or the whole leaf is dead;

Investigation method of panicle neck blast: sampling at five diagonal points in each plot, surveying 5 rice clumps at each point, surveying 25 clumps of all rice in each plot, recording the total number of spikes, number of diseased spikes and disease severity.

Grading standard for panicle blast (in panicle):

Level 0: no disease;

Level 1: less than 5% loss per panicle (individual branch disease);

Grade 3: loss of 6-20% per ear (about 1/3 of the branches are onset);

Grade 5: loss of 21-50% per ear (neck or main shaft diseased, grains half-shrunk);

Grade 7: loss of 51-70% per ear (ear neck disease, most of the shriveled grains);

Grade 9: loss of 71-100% per ear (neck disease, resulting in white ears).

Table 1 Field efficacy test of wettable powder for controlling rice blast

It can be seen from Table 1 that the control effect of the composition of the present invention on rice blast is significantly higher than that of the control agent, and it is safe for the growth of rice, and no adverse reactions are found in the field. in,

Example 6

2g polyoxin, 10 billion live spores/gram Bacillus amyloliquefaciens 10g, 4g sodium lauryl sulfate, 4g sodium lignosulfonate, 5g glucose, 1g ascorbic acid, kaolin to make up 100g to make 2% polyantimycin Su · 10 billion live spores/gram Bacillus amyloliquefaciens wettable powder.

Example 7

5g polyoxin, 10g 5 billion live spores/gram Bacillus amyloliquefaciens, 4g Sapindus powder, 3g sodium lignosulfonate, 3g glucose, 1% ascorbic acid, kaolin make up 100g, and make 10% polyoxin. 5 billion live spores/gram Bacillus amyloliquefaciens wettable powder.

Example 8

10g polyoxin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g sodium lauryl sulfate, 5g sodium lignosulfonate, 5g glucose, 3g urea, kaolin supplement 100g to make 10% polyantimycin Su · 20 billion live spores/g Bacillus amyloliquefaciens wettable powder.

Example 9

15g of polyoxin, 15 billion live spores/gram of Bacillus amyloliquefaciens 10g, 4g of sodium lauryl sulfate, 3g of sodium lignosulfonate, 3g of glucose, 1g of ascorbic acid, 100g of kaolin to make up 15% polyoxin Su · 15 billion live spores/gram Bacillus amyloliquefaciens wettable powder.

Example 10

20g polyoxin, 500 million live spores/gram Bacillus amyloliquefaciens 10g, 4g Sapindus powder, 3g sodium lignosulfonate, 3g glucose, 1% ascorbic acid, kaolin supplement 100g, made 20% polyoxin ·500 million live spores/gram Bacillus amyloliquefaciens wettable powder.

The wettable powders of embodiments 6 to 10 are all made by the following methods:

Mix all kinds of raw materials together, after jet crushing, mix and stir in a cone mixer for 30 minutes to obtain wettable powder.

Test example 2

Table 2 Field efficacy test of wettable powder for controlling ginseng root rot

It can be seen from Table 2 that the composition of the present invention has significant control effects on ginseng root rot, significantly higher than that of the control agent, and is safe for ginseng growth, with no adverse reactions found in the field.

Test example 3

Field test of polyoxin-Bacillus amyloliquefaciens wettable powder to control apple tree spotted leaf disease, polyoxin-Bacillus amyloliquefaciens wettable powder was diluted 1000 times to spray apple trees in spring and autumn shoots Apply to prevent and control apple tree spot defoliation disease.

Time and frequency of spraying: 6 sprayings in total, the first spraying on May 20th, and then on June 10th, June 25th, July 10th, July 25th, and August 5th medicine.

Survey time and method: base survey before spraying (May 20), no disease occurred in the whole garden before spraying, two surveys on June 25 and August 15 respectively, a total of 3 surveys.

5 trees in each plot are surveyed, and each plant is fixed with two vigorous branches in the five directions of east, south, west, north, and middle, and regular surveys are carried out during the period when the spring stops and the autumn shoots stop. , Record the total number of leaves and the number of diseased leaves at all levels.

Grading standards: Grade 0: No lesion; Grade 1: Lesion area accounts for less than 10% of the entire leaf area; Grade 3: Lesion area accounts for 11%-25% of the entire leaf area; Grade 5: Lesion area accounts for the entire leaf area 26% to 40% of the leaf area; Grade 7: the lesion area accounts for 41% to 65% of the entire leaf area; Grade 9: the lesion area accounts for more than 66% of the entire leaf area.

Table 3 Field efficacy test of wettable powder for controlling apple tree spot defoliation

As can be seen from Table 3, the composition of the present invention has a significant preventive effect on apple tree spot defoliation, which is significantly higher than that of the contrast agent, and is safe for the growth of apple trees, and no adverse reactions have been found in the field.

Test example 4

Field test of polyoxin·Bacillus amyloliquefaciens wettable powder in the control of apple tree rot.

Table 4 Field efficacy test of wettable powder for controlling apple tree rot

Smear and control the rot of apple trees in 2015, set 4 repetitions in each place, set 5 trees in each plot, and the number of disease scars should not be less than 10. Polyoxin-Bacillus amyloliquefaciens wettable powder was diluted 100 times and starch and 3% naphthalene acetic acid solution were added to the solution to make a paste. After scraping off the rot on the branches of apple trees with a scraper, the The composition containing the invention is adjusted into a paste, and the method of smearing is evenly applied to the lesion site.

The concentration of Bacillus amyloliquefaciens in Table 4 is 1 billion live spores/g. The above field tests show that the compound preparation of polyoxin and Bacillus amyloliquefaciens has significantly better control effect on apple tree rot than a single agent. According to the visual inspection in the field, within the test dose range, the crops grew normally and no phytotoxicity occurred.

Example 11

2g kasugamycin, 1 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g calcium dodecylbenzenesulfonate, 3g lignosulfonate code 201107, 3g propylene glycol, 0.5g silicone oil, 3g glucose, 15g urea, 100g of water was supplemented, and 2% kasugamycin·1 billion live spores/gram Bacillus amyloliquefaciens aqueous preparation was prepared.

Example 12

4g kasugamycin, 5 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g pull open powder BX, 3g lignosulfonate code 201108, 3g ethylene glycol, 0.3g C8-10 fatty alcohol, 3g ammonium sulfate, 0.3 100 g of hydroxymethyl cellulose and water were supplemented to prepare 4% kasugamycin·5 billion live spores/gram of Bacillus amyloliquefaciens aqueous agent.

Example 13

4g kasugamycin, 10 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g pull open powder BX, 3g lignosulfonate code number 201107, 3g ethylene glycol, 0.3g C8-10 fatty alcohol, 4g sodium fulvic acid , 0.3g hydroxymethyl cellulose, water make up 100g, make 4% kasugamycin · 10,000,000,000 living spores/gram bacillus amyloliquefaciens aqueous agent.

Example 14

6g kasugamycin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 5g calcium dodecylbenzenesulfonate, 3g lignosulfonate code 201107, 4g glycerol, 0.5g silicone oil, 6g glucose, 0.3 100 g of hydroxymethyl cellulose and water were supplemented to prepare 6% kasugamycin·20 billion live spores/gram of Bacillus amyloliquefaciens aqueous agent.

Example 15

2g kasugamycin, 40 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g calcium dodecylbenzenesulfonate, 4g lignosulfonate code 201107, 4g glycerol, 1g silicone oil, 15g glucose, 0.3g Hydroxymethyl cellulose and water make up 100g to prepare 2% kasugamycin·40 billion live spores/gram of Bacillus amyloliquefaciens aqueous agent.

The formula of embodiment 11,12,13,14,15, active ingredient kasugamycin and bacillus amyloliquefaciens are mixed with wetting agent, then add other components such as dispersant, antifreeze, antifoaming agent, mix evenly, finally After adding water and fully stirring, the finished water preparation can be obtained.

Test example 5

Embodiments 11 to 15, 2% kasugamycin, 1 billion live spores/gram Bacillus amyloliquefaciens and a blank control are treated with a total of 8 treatments, each treatment is repeated 3 times, each repeats 30m ² , kasugamycin · Bacillus amyloliquefaciens Dilute the solution 1000 times with water, and start spraying at the early stage of cucumber bacterial angular spot disease;

experiment method

The test was carried out in the cucumber field of Jingyang County, Xianyang City, Shaanxi Province. The test variety was Jinyan No. 4, and 5 points were surveyed on double diagonal lines in each plot, and 3 cucumber plants were surveyed at each point.

Use the grading method to record the severity of the disease, and the grading standards are as follows:

Level 0: no disease;

Grade 1: The lesion pile area accounts for less than 5% of the whole leaf area;

Grade 3: The lesion pile area accounts for less than 6%-10% of the whole leaf area;

Grade 5: The lesion area accounts for less than 11%-20% of the whole leaf area;

Level 7: The lesion area accounts for less than 21%-50% of the whole leaf area;

Grade 9: The lesion area accounts for more than 50% of the whole leaf area.

Survey time and frequency

A total of 4 investigations were conducted in the test, including base investigation before the drug, 7 days after the first drug, 7 days after the second drug and 15 days after the drug.

Drug efficacy calculation method:

Diseased leaf rate (%) = number of diseased leaves/total number of surveyed leaves × 100;

Disease index = ∑ (number of diseased leaves at all levels × relative level value) / (total number of leaves under investigation × 9) × 100;

Control effect (%)=[1-(pre-medication disease index of blank control area×post-medication disease index of treatment area)/(post-medication disease index of blank control area×pre-medication disease index of treatment area)]×100.

Method for investigation of phytotoxicity: 15 consecutive days after application, visually inspect whether the medicament has phytotoxicity to the crops.

Test results and analysis:

Table 5 Field efficacy test of water agent for controlling bacterial angular spot of cucumber

The concentration of Bacillus amyloliquefaciens in Table 5 is 1 billion live spores/g. The above field experiments show that the compound preparation of kasugamycin and Bacillus amyloliquefaciens has significantly better control effect on bacterial angular spot of cucumber than a single agent. According to the visual observation in the field, within the range of the test dose, the crops grow normally and no phytotoxicity occurs, which is of great significance for increasing agricultural production.

Example 16

0.5g Zhongshengmycin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g calcium dodecylbenzenesulfonate, 3g lignosulfonate code 201107, 3g propylene glycol, 0.5g silicone oil, 3g glucose, 0.5 100 g of urea and water are added to prepare 0.5% Zhongshengmycin · 20 billion live spores/gram of Bacillus amyloliquefaciens aqueous agent.

Example 17

1g Zhongshengmycin, 5 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g pull open powder BX, 3g lignosulfonate code 201108, 3g ethylene glycol, 0.3g C8-10 fatty alcohol, 3g ammonium sulfate, 0.3 g of hydroxymethyl cellulose and 100 g of water were added to prepare 1% Zhongshengmycin · 5 billion live spores/gram of Bacillus amyloliquefaciens aqueous agent.

Example 18

2g Zhongshengmycin, 10 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g pull open powder BX, 3g lignosulfonate code 201107, 3g ethylene glycol, 0.3g C8-10 fatty alcohol, 4g fulvic acid Sodium, 0.3g hydroxymethylcellulose, and water make up 100g to prepare 2% Zhongshengmycin · 10 billion live spores/gram of Bacillus amyloliquefaciens aqueous agent.

Example 19

3g Zhongshengmycin, 5 billion live spores/gram Bacillus amyloliquefaciens 10g, 5g calcium dodecylbenzenesulfonate, 3g lignosulfonate code number 201107, 4g glycerol, 0.5g silicone oil, 6g glucose, 0.3 g of hydroxymethylcellulose and 100 g of water were added to prepare 3% Zhongshengmycin·5 billion live spores/gram of Bacillus amyloliquefaciens aqueous agent.

Example 20

4g Zhongshengmycin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g calcium dodecylbenzenesulfonate, 4g lignosulfonate code 201107, 4g glycerol, 1g silicone oil, 15g glucose, 0.3 100 g of hydroxymethyl cellulose and water are supplemented to prepare 4% Zhongshengmycin · 20 billion live spores/gram Bacillus amyloliquefaciens water.

For the formulations of Examples 16, 17, 18, 19, and 20, the active ingredients Zhongshengmycin and Bacillus amyloliquefaciens are completely dissolved with a cosolvent, and then other ingredients such as dispersants, antifreeze agents, and defoamers are added, and mixed evenly , and finally add water, after fully stirring, the finished water preparation can be obtained.

Test example 6

Embodiments 16 to 20, 1% Zhongshengmycin water preparation, 1 billion live spores/gram of Bacillus amyloliquefaciens and blank control were treated with a total of 8 treatments, each treatment was repeated 3 times, each repeated 30m ² , Zhongshengmycin· Bacillus amyloliquefaciens water agent prevents rice bacterial blight at 50 g/mu, and the water consumption per mu is 45 kg. Spraying starts at the early stage of rice bacterial blight.

experiment method

The experiment was conducted in Chenggu County, Hanzhong City, Shaanxi Province, and 5 points were surveyed on double diagonal lines in each plot. During the survey, all leaves of five plants were surveyed, and the occurrence of diseases was counted.

Use the grading method to record the severity of the disease, and the grading standards are as follows:

Grade 0: No disease; Grade 1: Lesion area accounts for less than 5% of the whole leaf area;

Grade 3: The lesion pile area accounts for less than 6%-10% of the whole leaf area;

Grade 5: The lesion area accounts for less than 11%-20% of the whole leaf area;

Level 7: The lesion area accounts for less than 21%-50% of the whole leaf area;

Grade 9: The lesion area accounts for more than 50% of the whole leaf area.

Investigation time and method

A total of 4 investigations were conducted in the test, namely the investigation of the disease index before the drug, the first 7-day control effect investigation after the drug, and the 2nd 7-day and 15-day control effect investigation after the drug.

Drug efficacy calculation method:

Diseased leaf rate (%) = number of diseased leaves/total number of surveyed leaves × 100; disease index = ∑ (number of diseased leaves at all levels × relative level value) / (total number of surveyed leaves × 9) × 100; control effect (%) =[1-(pre-drug condition index of blank control area×post-drug condition index of treatment area)/(post-drug condition index of blank control area×pre-drug condition index of treatment area)]×100.

Method for investigation of phytotoxicity: visually inspect whether the medicament has phytotoxicity to the crops for 7 consecutive days after application.

Test Results and Analysis

Table 6 Field efficacy test of water agent for controlling rice bacterial blight

The concentration of Bacillus amyloliquefaciens in Table 6 is 1 billion live spores/g. The above field tests show that the compound preparation of Zhongshengmycin and Bacillus amyloliquefaciens has significantly better control effect on rice bacterial blight than a single agent. According to the visual inspection in the field, within the range of the test dose, the crops grow normally and no phytotoxicity occurs. With the increase of the content of Bacillus amyloliquefaciens, the yield retention rate of rice increases significantly.

Example 21

2g kasugamycin, 10 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g polycarboxylate codenamed LG-3, 3g calcium dodecylbenzenesulfonate, 3g sodium bicarbonate, 5g starch, 1g xanthan gum , Filling balance;

Example 22

5g of kasugamycin, 15 billion live spores/gram of Bacillus amyloliquefaciens 10g, 6g of polycarboxylate dispersant GY-D1252, 15g of sodium lauryl sulfate, 5g of bentonite, 3g of starch, 0.5g of ascorbic acid, and the balance of kaolin.

Example 23

6g of kasugamycin, 20 billion live spores/gram of Bacillus amyloliquefaciens 10g, 5g of lignosulfonate code number 201107, 5g of Sapinberry powder, 5g of starch, 0.5g of Congo red, and the balance of kaolin.

Example 24

4g kasugamycin, 5 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g polycarboxylate codenamed LG-3, 3g calcium dodecylbenzenesulfonate, 3g sodium bicarbonate, 5g starch, 1g xanthan gum , Kaolin balance.

Example 25

4g kasugamycin, 500 million live spores/gram of Bacillus amyloliquefaciens 10g, 4g polycarboxylate dispersant GY-D1252, 3g sodium lauryl sulfate, 5g bentonite, 3g starch, 0.5g ascorbic acid, clay balance.

The water-dispersible granules in Examples 21, 22, 23, 24, and 25 are produced by the following method: mix the various raw materials in the formula evenly, pulverize them with an ultra-fine jet mill, knead, and then add them to the fluidized bed to make After granulation, drying and sieving in a granulation dryer, the water-dispersible granules of the composition of the present invention are prepared through sampling and analysis.

Test Example 7

Test location: Xunwu County, Ganzhou City, Jiangxi Province. Embodiment 21 to 25, 6% kasugamycin, 1,000,000,000 live spores/gram bacillus amyloliquefaciens and blank control totally 8 are processed, and every processing repeats 4 times, and each repeats has 5 mature trees, kasugamycin. Bacillus amyloliquefaciens water-dispersible granules are diluted 1000 times to prevent and treat citrus canker, and spray at the early stage of citrus canker.

Test method: The directional spray method is adopted, and the time interval between the first spraying and the second spraying is 7 days. Survey method: survey 5 plants in each plot, take 5 points on each plant, select 10 fruits and two leaf tips at each point, and investigate once before spraying and once 20 days after the first spraying.

Grading method:

Level 0: no disease;

Grade 1: Each leaf (fruit) has 1-5 diseased spots;

Grade 3: Each leaf (fruit) has 6-10 diseased spots;

Grade 5: Each leaf (fruit) has 11-15 diseased spots;

Level 7: Each leaf (fruit) has 16-20 diseased spots;

Grade 9: Each leaf (fruit) has more than 21 diseased spots.

Drug efficacy calculation method:

Diseased leaves (fruits) rate (%) = number of diseased leaves (fruits)/total number of surveyed leaves (fruits) × 100;

Disease index = ∑ (number of diseased leaves (fruits) at all levels × relative level value) / (total number of leaves (fruits) under investigation × 9) × 100;

Control effect (%)=[1-(pre-medication disease index of blank control area×post-medication disease index of treatment area)/(post-medication disease index of blank control area×pre-medication disease index of treatment area)]×100.

In Table 7, the concentration of Bacillus amyloliquefaciens is 1 billion live spores/gram field test shows. The results showed that the compound preparation of kasugamycin and Bacillus amyloliquefaciens was significantly better than single agent in the control of citrus canker. According to the visual inspection in the field, within the test dose range, the crops grew normally and no phytotoxicity occurred.

Table 7 Water-dispersible granules control citrus canker field efficacy test results

Example 26

1.5g polyoxin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g polycarboxylate codenamed LG-3, 3g calcium dodecylbenzenesulfonate, 3g sodium bicarbonate, 5g starch, 1g yellow The balance of raw gum and kaolin.

Example 27

3g kasugamycin, 15 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g polycarboxylate dispersant GY-D1252, 5g sodium lauryl sulfate, 5g bentonite, 3g starch, 0.5g ascorbic acid, clay balance;

Example 28

5g kasugamycin, 10 billion live spores/gram Bacillus amyloliquefaciens 10g, 5g lignosulfonate code number 201107, 5g Sapinberry powder, 5g starch, 0.5g Congo red, the rest of kaolin;

Example 29

10g polyoxin, 10 billion live spores/gram Bacillus amyloliquefaciens 10g, 6g polycarboxylate codenamed LG-3, 3g calcium dodecylbenzenesulfonate, 3g sodium bicarbonate, 5g starch, 1g xanthan Glue and clay balance;

Example 30

15g polyoxin, 10 billion live spores/gram Bacillus amyloliquefaciens 10g, 4g polycarboxylate dispersant GY-D1252, 3g sodium lauryl sulfate, 5g bentonite, 3g starch, 0.5g ascorbic acid, the balance of kaolin ;

For the formulas of Examples 26, 27, 28, 29, and 30, mix the formula materials evenly, pulverize them with an ultra-fine jet mill, knead them, and then add them to a fluidized bed granulation dryer for granulation, drying, and sieving After sampling and analysis, the water-dispersible granules of the composition of the present invention were prepared.

Test Example 8: Water Dispersible Granules Control Apple Moldy Heart Disease

Embodiment 26 to 30, 10% polyoxin, 1,000,000,000 live spores/gram bacillus amyloliquefaciens and blank control total 8 treatments, 5 apple trees in every plot, randomly arranged, every treatment repeats 4 times, and the test is sprayed altogether The medicine was sprayed 2 times, once at the full flowering stage and the end of flowering stage of the apples, and the polyoxin·Bacillus amyloliquefaciens water-dispersible granules were diluted 1000 times to prevent and control apple mold core disease. After the apples matured, 5 points were randomly selected in each plot. Pick 20 apples at each point, cut them open to investigate the incidence of fruit, and calculate the control effect.

Test location: Luochuan County, Yan'an City, Shaanxi Province.

Control effect = (the diseased fruit rate of the clear water control - the diseased fruit rate of the treatment) / the diseased fruit rate of the clear water control.

Table 8 Water-dispersible granules control field efficacy test results of apple moldy heart disease

The above field experiments showed that the compound preparation of polyoxin and Bacillus amyloliquefaciens was significantly better than a single agent in the control of apple moldy heart disease. After 2 times of application in the apple flowering stage, it has a significant control effect on apple moldy heart disease. Polyoxin and Bacillus amyloliquefaciens have obvious mutual synergistic effect on controlling apple mold core. Observed on the first day after spraying and several days after spraying to the apple picking period, the growth and development of apples in the polyoxin-Bacillus amyloliquefaciens treatment area was normal, and no adverse reactions were seen.

Example 31

1.5g polyoxin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g glycerol fatty acid ester polyoxyethylene ether, 4g Sapinberry powder, 2g aluminum magnesium silicate, 0.2g silicone oil, 5g potassium fulvic acid , 1g urea, castor oil supplement 100g.

Example 32

1.5g polyoxin, 30 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g glycerol fatty acid ester polyoxyethylene ether, 4g Sapinberry powder, 2g aluminum magnesium silicate, 0.2g silicone oil, 5g potassium fulvic acid , 1g urea, castor oil supplement 100g.

Example 33

3g polyoxin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g glycerol fatty acid ester polyoxyethylene ether, 4g Sapinberry powder, 2g aluminum magnesium silicate, 0.2g silicone oil, 5g ammonium sulfate, 1g urea , castor oil make up 100g.

Example 34

3g polyoxin, 30 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g glycerol fatty acid ester polyoxyethylene ether, 4g sapinberry powder, 2g aluminum magnesium silicate, 0.2g silicone oil, 5g ammonium sulfate, 1g urea , castor oil make up 100g.

Example 35

5g polyoxin, 20 billion live spores/gram Bacillus amyloliquefaciens 10g, 3g glycerol fatty acid ester polyoxyethylene ether, 4g Sapinberry powder, 2g aluminum magnesium silicate, 0.2g silicone oil, 5g ammonium sulfate, 1g urea , castor oil make up 100g.

For the formulations of Examples 31, 32, 33, 34, and 35, the active ingredient polyoxin is mixed with Bacillus amyloliquefaciens, dispersant, stabilizer, defoamer and solvent according to the proportion of the formulation, and put into a sand mill After internal grinding, it is sent into a homogeneous mixer and mixed to obtain a finished product, which is made into granules of the composition of the present invention.

Test example 9

Embodiments 31 to 35, 10% polyoxin, 1 billion live spores/Bacillus amyloliquefaciens and a blank control were treated with a total of 8 treatments, each plot area was 15m ² , and each treatment was repeated 4 times. In the initial stage of disease occurrence, the poisonous soil, Investigate the disease index before the poisonous soil, investigate the disease index 7 days and 14 days after the poisonous soil, take 5 points according to the diagonal line in each plot during the investigation, investigate 5 plants at each point, investigate all leaves, record the diseased plants, disease level, The disease index was calculated, and the yield of the tubers was harvested after maturity.

Use the grading method to record the severity of the disease, and the grading standards are as follows:

Level 0: no disease;

Grade 1: The lesion pile area accounts for less than 5% of the whole leaf area;

Grade 3: The lesion pile area accounts for less than 6%-10% of the whole leaf area;

Grade 5: The lesion area accounts for less than 11%-20% of the whole leaf area;

Level 7: The lesion area accounts for less than 21%-50% of the whole leaf area;

Grade 9: The lesion area accounts for more than 50% of the whole leaf area.

Calculation method of drug efficacy: diseased leaf rate (%) = number of diseased leaves/total number of surveyed leaves × 100;

Condition index = ∑ (condition index at all levels × relative level value) / (total number of leaves under investigation × 9) × 100;

Control effect (%)=[1-(pre-medication disease index of blank control area×post-medication disease index of treatment area)/(post-medication disease index of blank control area×pre-medication disease index of treatment area)]×100.

Method for investigation of phytotoxicity: visually inspect whether the medicament has phytotoxicity to the crops for 7 consecutive days after application.

The test results and analysis are as follows:

Table 9 granules control potato late blight field efficacy test results

The above field experiments showed that polyoxin and Bacillus amyloliquefaciens granule poisonous soil had better control effect on potato late blight than single agent. Potato late blight has a significant control effect on potato late blight at the early stage of onset. Polyoxin and Bacillus amyloliquefaciens have obvious synergistic effect on controlling potato late blight. Observed on the 7th day after poisoning the soil and several days after the drug treatment to the potato harvest period, the growth and development of the potatoes were normal, and no adverse reactions were seen. The test result of the potato output of medication group and blank control group is as shown in table 10, as can be seen from table 10, the polyoxin bacillus amyloliquefaciens granule provided by the embodiment of the present invention 31～35 is to suffering from potato late blight. Potatoes have a yield-increasing effect, which is better than that of a single dose.

Table 10 The yield results of each treatment of granule control potato late blight test

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims (10)

1. A bactericidal composition, characterized in that, the bactericidal active ingredient of the bactericidal composition mainly consists of bacillus amyloliquefaciens and antibiotics with a weight ratio of 10:0.5～20; the antibiotics include kasugamycin, polyclonal Any one of mycin and Zhongshengmycin; the number of viable spores of the Bacillus amyloliquefaciens is 5 to 50 billion/g. 2. bactericidal composition according to claim 1, is characterized in that, the ratio of described Bacillus amyloliquefaciens weight and described antibiotic weight is 10:3.5～7, and the viable spore number of described Bacillus amyloliquefaciens is 50 ~40 billion/g. 3. bactericidal composition according to claim 1, is characterized in that, the bactericidal active substance of described bactericidal composition comprises bacillus amyloliquefaciens and kasugamycin, The ratio of the weight of the Bacillus amyloliquefaciens to the weight of the kasugamycin is 10:2-10, and the number of live spores of the Bacillus amyloliquefaciens is 500 million-40 billion/g. 4. bactericidal composition according to claim 1, is characterized in that, the bactericidal active substance of described bactericidal composition comprises bacillus amyloliquefaciens and polyoxin, The ratio of the weight of the Bacillus amyloliquefaciens to the weight of the polyoxin is 10:1.5-20, and the number of live spores of the Bacillus amyloliquefaciens is 500 million-30 billion/g. 5. bactericidal composition according to claim 1, is characterized in that, the bactericidal active substance of described bactericidal composition comprises bacillus amyloliquefaciens and Zhongshengmycin, The ratio of the weight of the Bacillus amyloliquefaciens to the weight of the Zhongshengmycin is 10:0.5-4, and the number of live spores of the Bacillus amyloliquefaciens is 5 billion-20 billion/g. 6. The application of the bactericidal composition according to claim 1 in the prevention and treatment of crop diseases, characterized in that, the crop diseases include rice blast, ginseng root rot, apple spot defoliation, apple tree rot, cucumber bacterial Angular spot, rice white streak blight, citrus canker, apple mold core and potato late blight. 7. A bactericidal preparation, characterized in that it comprises the bactericidal composition according to claim 1, the amount of the bactericidal composition accounts for 10.5 to 30% of the weight of the bactericidal preparation, and the balance is a pesticide acceptable As an auxiliary material, the bactericidal preparation is one of wettable powder, aqueous solution, suspension concentrate, water-dispersible granule or granule. 8. The bactericidal preparation according to claim 7, characterized in that, the auxiliary material includes a UV protecting agent, and the amount of the UV protecting agent accounts for 0.1 to 15% of the weight of the bactericidal preparation, preferably, the UV protecting The agent includes one or more of carboxymethylcellulose, urea, starch, dextrin, Congo red, ascorbic acid, xanthan gum, and pure milk. 9. The bactericidal preparation according to claim 8, characterized in that, when the bactericidal preparation is a wettable powder, by weight, it comprises 12-30% of the bactericidal composition, 0.5-3% of a UV protective agent, a moistening 3-15% of agent, 2-15% of dispersant, 3-15% of nutrient, and the rest is filler; When the bactericidal preparation is an aqueous solution, by weight, it includes 10.5-14% of the bactericidal composition, 0.3-0.5% of an ultraviolet protective agent, 3-6% of a wetting agent, 5-20% of a dispersant, and 1-20% of an antifreeze agent. 5%, thickener 0.1-2%, defoamer 0.1-3%, nutrient 1-15%, the balance is water; When the bactericidal preparation is a water-dispersible granule, by weight, it includes 11.5-25% of the bactericidal composition, 0.5-1% of an ultraviolet protective agent, 3-10% of a dispersant, 1-10% of a wetting agent, a disintegrating 1 to 5% of the agent, 1 to 15% of the nutritional agent, and the rest is filler; When the bactericidal preparation is a granule, by weight, it includes 11.5-15% of the bactericidal composition, 0.5-1% of an ultraviolet protective agent, 1-10% of a dispersant, and 01-5% of a thickener. %, defoamer 0.1-5%, nutrient 1-10%, and the rest is castor oil; When the bactericidal preparation is a suspension, by weight, it includes 10-20% of the bactericidal composition, 0.1-2% of an ultraviolet protector, 5-20% of a dispersant, 1-5% of an antifreeze, and 0.1% of a thickener. ～2%, defoamer 0.1～3%, nutrient 1～15%, the balance is water. 10. The bactericidal preparation according to claim 9, characterized in that, when the bactericidal preparation is a wettable powder, The wetting agent includes one or more of Sapindus chinensis powder, silkworm excrement, sodium dodecylbenzenesulfonate, washing powder, and the dispersant includes lignin, lignosulfonate, alkylsulfonic acid One or more of salt, the nutrient includes one or more of glucose, lactose, sucrose, potassium fulvate, sodium fulvate, ammonium sulfate; When the bactericidal preparation is an aqueous solution, the wetting agent includes calcium dodecylbenzenesulfonate and/or pull-off powder BX, and the dispersant includes lignosulfonate, alkylphenol polyoxyethylene ether methyl ether One or more of condensate sulfate, alkyl sulfonate calcium salt, naphthalene sulfonate formaldehyde condensate sodium salt, glycerin fatty acid ester polyoxyethylene ether, and the antifreeze includes ethylene glycol, propylene glycol, propylene glycol One or more of triols, the defoamer includes one or more of silicone oil, silicone compounds, C10-20 saturated fatty acid compounds, C8-10 fatty alcohols, and the nutrient includes glucose, One or more of lactose, sucrose, potassium fulvate, sodium fulvate, ammonium sulfate; When the bactericidal preparation is a water-dispersible granule, the dispersant includes lignosulfonate, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, alkylsulfonate calcium salt, naphthalenesulfonic acid formaldehyde condensate One or more of sodium salt, alkylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester, fatty amine polyoxyethylene ether, glycerin fatty acid ester polyoxyethylene ether, the wetting agent includes lauryl sulfuric acid One or more of sodium, calcium dodecylbenzenesulfonate, pull open powder BX, saponin powder, silkworm excrement, sapindus powder, and the nutritional agent is glucose, lactose, sucrose, potassium fulvic acid 1. One or more of sodium fulvic acid and ammonium sulfate, and the disintegrating agent includes one or more of bentonite, ammonium sulfate, aluminum chloride, citric acid, succinic acid, and sodium bicarbonate; When the bactericidal preparation is a granule, the dispersant includes polycarboxylate, lignosulfonate, alkylsulfonate calcium salt, naphthalenesulfonic acid formaldehyde condensate sodium salt, alkylphenol polyoxyethylene ether, One or more of fatty acid polyoxyethylene ester, fatty amine polyoxyethylene ether, glycerin fatty acid ester polyoxyethylene ether, the wetting agent includes sodium lauryl sulfate, calcium dodecylbenzenesulfonate, One or more of pulling apart powder BX, saponin powder, silkworm excrement, and Sapindus chinensis powder, and the thickener includes carboxymethyl cellulose, carboxyethyl cellulose, methyl cellulose, silicic acid Aluminum magnesium, one or more of polyvinyl alcohol, the defoamer includes one or more of silicone oil, silicone compounds, C10-20 saturated fatty acid compounds, C8-10 fatty alcohol, the nutrient One or more of glucose, lactose, sucrose, potassium fulvate, sodium fulvate, and ammonium sulfate; When the bactericidal preparation is a suspension, the dispersant is one or more of polycarboxylate, alkylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester, fatty amine polyoxyethylene ether, and the antifreeze The agent is one or more of ethylene glycol, propylene glycol, and glycerol, and the thickener includes carboxymethyl cellulose, carboxyethyl cellulose, methyl cellulose, magnesium aluminum silicate, polyvinyl alcohol One or more of them, the defoamer is one or more of silicone oil, silicone compound, C10-20 saturated fatty acid compound, C8-10 fatty alcohol, and the nutrient is glucose, lactose, sucrose , potassium fulvate, sodium fulvate, and ammonium sulfate.