CN105679165A - Human placenta extracorporeal circulation irrigating box - Google Patents

Abstract

The invention belongs to the technical field of medical experimental study equipment, and relates to a human placenta extracorporeal circulation irrigating box. The irrigating box is mainly composed of three parts, namely a box body, a box cover and a bracket, wherein the box cover is arranged on the box body; the box body is fixedly arranged on the bracket; the box body is composed of an outer wall and an inner wall; and a hollow cavity is formed between the inner wall and the outer wall. Based upon results of using, a human placenta extracorporeal circulation irrigating model, which is constructed with the participation of the human placenta extracorporeal circulation irrigating box disclosed by the invention, is relatively good in physiological completeness and metabolic stability; and the irrigating model is simple in structure and high in space utilization rate, capable of achieving commercial production and convenient to unify model and experimental platform standard.

Description

A kind of human placenta extracorporeal circulation perfusion box

technical field

The invention belongs to the technical field of medical experiment research equipment, and relates to a human placenta extracorporeal circulation perfusion experiment research device, in particular to a human placenta extracorporeal circulation perfusion box.

Background technique

In the 1960s, Krantz first developed the human placental extracorporeal circulation perfusion model for the study of placental permeability. After two improvements by Schneider and Brandes, it was simplified as placental lobular perfusion, which improved the success rate of normal operation of the model. Compared with animal experiments or other in vitro models (such as BeWo cell model, placental tissue model, etc.), the extracorporeal circulation perfusion model of human placenta is more compliant with medical ethics, has no species difference restrictions, and retains the complete human placental structure, It restores the blood circulation of the mother, placenta and fetus as realistically as possible, and is the only model for studying the placental barrier function at the organ level. At present, the above model is mainly used in the following two aspects:

1. Placental transfer of drugs or physiologically active substances. A normal full-term placenta was used to construct an extracorporeal circulation model, and the drug to be tested was added to one side of the pool, and the changes in the composition of the perfusate and other set indicators in the other side of the pool were analyzed to investigate the placental transfer. Drugs currently involved include statins, anesthetics, antiviral drugs, antibiotics, hypoglycemic drugs, and antineoplastic drugs, etc. The research content includes drug penetration/clearance, pharmacokinetics, and related transport proteins; In addition, other substances that mothers may be exposed to during pregnancy can also be used as research objects, such as caffeine, nicotine, benzoate, hormones and small molecular proteins, etc.;

2. Pathogenesis of diseases related to placental factors. By studying the placenta of a parturient with complications or changing the physical and chemical properties of the perfusate to simulate pathological conditions (such as hypoxia, inflammation, hypoproteinemia, acidemia, etc.), to investigate the effects of endogenous or exogenous factors on the placenta To study the possible pathophysiological mechanisms of related diseases (such as preeclampsia, intrahepatic cholestasis of pregnancy, etc.).

At present, although some laboratories have achieved certain research results in the application of the human placenta extracorporeal circulation perfusion model, there is still no unified evaluation standard in the world, and the specific details of the model, such as the method of establishing the circulation, the composition of the perfusate, and the perfusion rate, are also different. , it is difficult to compare and reproduce, and there are fewer studies on this model in my country; therefore, it is of great significance to design a scientific and convenient human placenta extracorporeal circulation perfusion device, establish an experimental platform on this basis, and carry out research on drugs and pathological mechanisms. Significance.

Contents of the invention

The object of the present invention is to overcome the defects or deficiencies of the prior art, and provide a human placenta extracorporeal circulation perfusion box; the human placenta extracorporeal circulation perfusion box has scientific design, simple structure and convenient use.

The human placenta extracorporeal circulation perfusion box of the present invention is characterized in that it is mainly composed of three parts: a box body 31, a box cover 32 and a bracket 33; the box cover 31 is placed on the box body 32, and the box body 32 is fixedly installed on the bracket 33;

The box body 32 is composed of an outer wall 2 and an inner wall 3, with a hollow cavity between the inner and outer walls;

In the present invention, the box body 32 is in the shape of a round basin and is made of transparent material (the condition of the placenta inside the container can be directly observed from the periphery); the box body 32 is composed of an inner wall and an outer wall, and there is a hollow cavity between the inner and outer walls. Pass into 37 ℃ circulating water;

The box body 32 is also provided with a box body edge 1, a box bottom 4, an inner wall inlaid raised ring 5, a supporting placenta cylinder 6, a circulating water inlet 7, a circulating water outlet 8, and a maternal circulating perfusion fluid collection pipeline interface 9;

The circulating water inlet 7 is arranged on the bottom of the box 4, on which a piston switch is arranged; the circulating water outlet 8 is arranged on the edge 1 of the box body;

The surface of the box body edge 1 is frosted, which can be closely attached to the box cover 31; the middle part of the box bottom 4 is a cylindrical hollow structure, which communicates with the outside and can support the placenta; the box bottom 4 It is an inclined surface, and the included angle between it and the inner wall 3 is 110°. The lowest part of the box bottom 4 is provided with a maternal circulating perfusion fluid collection pipeline interface 9 for collecting the maternal circulating perfusion fluid; the maternal circulating perfusion fluid collection pipeline A piston switch is provided on the interface 9;

The inner wall 3 is provided with four inner wall inlaid raised rings 5 for passing through the clips and rubber bands used for fixing the placenta;

The supporting placenta cylinder 6 is used to place the placenta;

In the present invention, the box cover 31 is made of transparent material (the condition of the placenta inside the container can be directly observed from the periphery), and the outer ring of the reverse side is frosted, which can be closely attached to the upper part of the edge 1 of the box body; the box cover 31 is provided with two A handle 11 is convenient for taking; the center of the box cover 31 and the middle part of the edge are respectively provided with a hole 12, which is used to pass through experimental equipment such as pipelines and thermometers, and to facilitate operations such as sampling and observation;

In the present invention, the bracket 33 is composed of a tray on the upper part of the bracket and four pillars 13; the tray is used to support the edge 1 of the box body, and its upper folded part is higher than the edge 1 of the box body, so as to block the translation of the box cover 31 The effect; the lower part of the bracket upper tray 10 is provided with a lower tray gap 14, which is used to pass through the joint of the circulating water outlet 7 of the box body.

During use, circulating water at 37°C circulates between the outer wall 2 and the inner wall 3, and the circulating water enters from the water inlet 7 and flows out from the water outlet 8 to achieve the best circulation effect; the placenta is placed on the supporting placenta cylinder 6, Placental leaflets used for perfusion face the opening to facilitate the establishment of fetal circulation pipelines; then, the edge of the flattened placenta is clamped with clips and fixed on the inner wall mosaic raised ring 5 by a rubber band; the operator Hold the handle 11 and cover the box cover 31 on the box body 32, so that the frosted outer ring on the back side is closely attached to the edge 1 of the box body, and make the No. 19 blunt head of the simulated uterine spiral artery The needle and the pipeline pass through the middle hole 12 (if necessary during the experiment, thermometers and other equipment can enter through the outer hole); then, the basin is placed on the stainless steel bracket supported by the pillar 13, and the tray 10 clamps the edge 1 of the box body, and the circulating water outlet 8 is connected to the constant temperature circulating water tank through the gap 14 in the lower part of the tray; a simulation experiment is carried out.

The results of use show that the extracorporeal circulation perfusion model of human placenta constructed by the present invention has good physiological integrity by detecting the leakage rate of fetal circulation, the penetration rate of antipyrine (tool drug) and the pH stability of placental perfusate And metabolic stability, and its simple structure, high space utilization, can realize commercial production, and facilitate the unification of model and experimental platform standards.

Compared with existing model devices, the present invention has the following advantages:

(1) The present invention adopts a double-wall design with circulating water inside, which can simulate the steady-state environment of the placenta in the mother's body and maintain the functional activity of the placenta during the experiment;

(2) The design of the box cover and the hollow cylinder of the box body of the present invention is convenient for placenta-related operations, and the inclined box bottom can more completely recover the maternal circulating perfusion fluid;

(3) The present invention has simple structure, high space utilization rate, can realize commercial production, and is convenient to unify the standard of model and experimental platform.

Description of drawings

Fig. 1: the sectional structure schematic diagram of the human placenta extracorporeal circulation perfusion box box body of the present invention,

Among them, 1 is the edge of the box body, 2 is the outer wall, 3 is the inner wall, 4 is the bottom of the box, 5 is the raised ring inlaid on the inner wall, 6 is the supporting placenta cylinder, 7 is the circulating water inlet, 8 is the circulating water outlet, 9 is the connection of the maternal circulation perfusion fluid collection pipeline, and 10 is the upper tray of the bracket.

Fig. 2: the structural representation of the lid of the human placenta extracorporeal circulation perfusion box lid of the present invention,

Wherein, 11 is a handle, and 12 is a hole.

Fig. 3: Schematic diagram of the structure of the human placenta extracorporeal circulation perfusion box support of the present invention,

Wherein, 13 is a pillar, and 14 is a gap at the lower part of the tray.

Figure 4: Schematic diagram of the appearance and structure of the extracorporeal circulation perfusion box for human placenta of the present invention.

Wherein, 31 is a box cover, 32 is a box body, and 33 is a support.

detailed description

For ease of understanding, the present invention will be described in detail below through specific drawings and embodiments. It should be pointed out that the specific examples and accompanying drawings are only for illustration. Obviously, those skilled in the art can make various amendments and changes within the scope of the present invention according to the description herein. These amendments and Modifications are also included within the scope of the present invention.

Example 1

As shown in Figures 1 to 4, my placenta extracorporeal circulation perfusion box is mainly composed of three parts: a box body 31, a box cover 32 and a bracket 33; the box cover 31 is placed on the box body 32, and the box body 32 fixedly installed on the bracket 33; the box body 32 is composed of an outer wall 2 and an inner wall 3, and the inner and outer walls are hollow;

The box body 32 is in the shape of a round basin and is made of transparent material (the placenta inside the container can be directly observed from the periphery); the box body 32 is composed of an inner wall and an outer wall. Recycled water;

The box body 32 is also provided with a box body edge 1, a box bottom 4, an inner wall inlaid raised ring 5, a supporting placenta cylinder 6, a circulating water inlet 7, a circulating water outlet 8, and a maternal circulating perfusion fluid collection pipeline interface 9;

The circulating water inlet 7 is arranged on the bottom of the box 4, on which a piston switch is arranged; the circulating water outlet 8 is arranged under the edge 1 of the box; the surface of the edge 1 of the box is frosted, which can The box cover 31 fits tightly; the middle part of the box bottom 4 is a cylindrical hollow structure, which communicates with the outside and can support the placenta; the box bottom 4 is an inclined surface, and the angle between it and the inner wall 3 is 110° , the lowest part of the box bottom 4 is provided with a maternal circulating perfusate collection pipeline interface 9 for collecting the maternal circulating perfusate; the maternal circulating perfusate collection pipeline interface 9 is provided with a piston switch; the inner wall 3 is provided with There are four raised rings 5 inlaid on the inner wall, which are used to pass through the clips and rubber bands used to fix the placenta; the supporting placenta cylinder 6 is used to place the placenta;

The box cover 31 is made of transparent material (the condition of the placenta inside the container can be directly observed from the periphery), and the outer ring of the reverse side is frosted, which can be closely attached to the upper part of the edge 1 of the box body; the box cover 31 is provided with two handles 11, It is convenient to take; the center of the box cover 31 and the middle part of the edge are respectively provided with a hole 12, which is used to pass through experimental equipment such as pipelines and thermometers, and to facilitate operations such as sampling and observation;

The support 33 is composed of a support upper tray and four pillars 13; the tray is used to support the box body edge 1, and its upper folded part is higher than the box body edge to block the translation of the box cover 31; the upper part of the support The lower part of the tray is provided with a notch 14 at the lower part of the tray, which is used to pass through the joint of the circulating water outlet 7 of the box body.

During use, circulating water at 37°C circulates between the outer wall 2 and the inner wall 3, and the circulating water enters from the water inlet 7 and flows out from the water outlet 8 to achieve the best circulation effect; the placenta is placed on the supporting placenta cylinder 6, Placental leaflets used for perfusion face the opening to facilitate the establishment of fetal circulation pipelines; then, the edge of the flattened placenta is clamped with clips and fixed on the inner wall mosaic raised ring 5 by a rubber band; the operator Hold the handle 11 and cover the box cover 31 on the box body 32, so that the frosted outer ring on the back side is closely attached to the edge 1 of the box body, and make the No. 19 blunt head of the simulated uterine spiral artery The needle and the pipeline pass through the middle hole 12 (if necessary during the experiment, thermometers and other equipment can enter through the outer hole); then, the basin is placed on the stainless steel bracket supported by the pillar 13, and the tray 10 clamps the edge 1 of the box body, and the circulating water outlet 8 is connected to the constant temperature circulating water tank through the gap 14 in the lower part of the tray; a simulation experiment is carried out.

The results of the above examples show that the human placenta extracorporeal circulation perfusion model that the present invention participates in constructing has better Physiological integrity and metabolic stability, and its simple structure, high space utilization, can realize commercial production, and facilitate the unification of model and experimental platform standards.

Claims (9)

1. A human placenta extracorporeal circulation perfusion box is characterized in that it is mainly composed of three parts: a box body (31), a box cover (32) and a bracket (33); the box cover (31) is placed on the box body (32), the box body (32) is fixedly installed on the bracket (33); the box body (32) is composed of an outer wall (2) and an inner wall (3), with a hollow cavity between the inner and outer walls. 2. The extracorporeal circulation perfusion box for human placenta according to claim 1, characterized in that the box body (32) is in the shape of a round basin and is made of transparent materials. 3. The human placenta extracorporeal circulation perfusion box according to claim 1 or 2, characterized in that, the box body (32) is provided with a box body edge (1), a wall (2), an inner wall (3), and a box bottom (4), the inner wall is inlaid with raised rings (5), the supporting placenta cylinder (6), the circulating water inlet (7), the circulating water outlet (8) and the maternal circulating perfusate collection pipeline interface (9). 4. The extracorporeal circulation perfusion box for human placenta according to claim 3, characterized in that, the circulating water inlet (7) is arranged on the bottom of the box (4), and a piston switch is arranged on it; the circulating water outlet Water outlet (8) is located at box body edge 1 below. 5. The human placenta extracorporeal circulation perfusion box according to claim 3, characterized in that, the middle part of the box bottom (4) is a cylindrical hollow structure, which communicates with the outside; the box bottom (4) is an inclined surface, The included angle between it and the inner wall (3) is 110°. 6. The human placenta extracorporeal circulation perfusion box according to claim 3, characterized in that, the bottom of the box (4) is provided with a maternal circulation perfusion fluid collection pipeline interface (9), and the maternal circulation perfusion fluid is collected A piston switch is provided on the pipeline interface (9). 7. The human placenta extracorporeal circulation perfusion box according to claim 3, characterized in that, the inner wall (3) is provided with an inner wall inlaid raised ring (5). 8. The human placenta extracorporeal circulation perfusion box according to claim 1, characterized in that, the box cover (31) is provided with a handle (11); the center of the box cover (31) and the middle part of the edge are respectively provided with holes (12). 9. The human placenta extracorporeal circulation perfusion box according to claim 1, characterized in that, the bracket (33) is composed of a bracket upper tray (10) and a pillar (13); the lower part of the bracket upper tray (10) is provided with There is a tray lower notch (14).