CN105665385B - Liquid transferring tube cleaner - Google Patents
Liquid transferring tube cleaner Download PDFInfo
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- CN105665385B CN105665385B CN201610135314.1A CN201610135314A CN105665385B CN 105665385 B CN105665385 B CN 105665385B CN 201610135314 A CN201610135314 A CN 201610135314A CN 105665385 B CN105665385 B CN 105665385B
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- 239000007788 liquid Substances 0.000 title description 5
- 238000004140 cleaning Methods 0.000 claims abstract description 85
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 81
- 239000007921 spray Substances 0.000 claims abstract description 8
- 238000005507 spraying Methods 0.000 claims description 9
- 239000004063 acid-resistant material Substances 0.000 claims description 3
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 230000003749 cleanliness Effects 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000011010 flushing procedure Methods 0.000 abstract description 4
- 230000036512 infertility Effects 0.000 abstract description 2
- 239000010902 straw Substances 0.000 description 8
- 239000008399 tap water Substances 0.000 description 5
- 235000020679 tap water Nutrition 0.000 description 5
- 229910021642 ultra pure water Inorganic materials 0.000 description 5
- 239000012498 ultrapure water Substances 0.000 description 5
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B9/00—Cleaning hollow articles by methods or apparatus specially adapted thereto
- B08B9/08—Cleaning containers, e.g. tanks
- B08B9/0804—Cleaning containers having tubular shape, e.g. casks, barrels, drums
- B08B9/0813—Cleaning containers having tubular shape, e.g. casks, barrels, drums by the force of jets or sprays
Landscapes
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Cleaning By Liquid Or Steam (AREA)
- Cleaning In General (AREA)
Abstract
本发明是移液管清洗装置,是一种主要用于在细胞分子生物学、微生物学、医学免疫学等领域实验中,对移液管等仪器进行清洗的实验辅助装置。实验无菌程度要求较高,清洗过程复杂、程序繁琐、费时费力。本发明所述装置具有分流结构,可将用于冲洗的水流分配到各分流管中,并采用形似试管的清洗管用于放置移液管,分流管向清洗管喷射水流对移液管内部进行清洗,水流在清洗管内回流完成对移液管外部的冲洗。清洗管上部设置溢流管将水排出,溢流管汇聚成到出水总管,将水排出。本发明所述移液管清洗装置结构简单、操作方便,所述清洗管具有回流功能,可对移液管内外部进行清洗,提高了移液管清洗的清洁度,同时提高了水的利用率。
The invention relates to a pipette cleaning device, which is an experimental auxiliary device mainly used for cleaning pipettes and other instruments in experiments in the fields of cell molecular biology, microbiology, medical immunology and the like. The experiment requires a high degree of sterility, and the cleaning process is complex, cumbersome, time-consuming and labor-intensive. The device of the present invention has a shunt structure, which can distribute the water flow used for flushing to each shunt pipe, and use a cleaning pipe shaped like a test tube to place the pipette, and the shunt pipe sprays water to the cleaning pipe to clean the inside of the pipette , the water flows back in the cleaning tube to complete the rinsing of the outside of the pipette. An overflow pipe is arranged on the upper part of the cleaning pipe to discharge the water, and the overflow pipes are converged into the water outlet main pipe to discharge the water. The pipette cleaning device of the present invention is simple in structure and easy to operate. The cleaning pipe has a backflow function and can clean the inside and outside of the pipette, thereby improving the cleanliness of the pipette cleaning and improving the water utilization rate at the same time.
Description
技术领域technical field
本发明涉及一种实验辅助装置,主要用于在细胞分子生物学、微生物学、医学免疫学等领域实验中,对移液管等仪器进行清洗。The invention relates to an experimental auxiliary device, which is mainly used for cleaning pipettes and other instruments in experiments in the fields of cell and molecular biology, microbiology, medical immunology and the like.
背景技术Background technique
分子生物学、微生物学、免疫学等学科的实验中,对设备、器材的无菌程度要求较高,其中使用量较大、且重复使用的仪器如移液管等,都需要严格的清洗、消毒、灭菌操作。一般的清洗过程是先经洗涤剂清洗,再泡酸12小时,然后分别使用自来水冲洗15次、纯净水冲洗10次、超纯水冲洗5次,之后放恒温烤箱烤干,最后高压消毒灭菌。整个操作过程复杂、程序繁琐,尤其是自来水、纯水及超纯水的冲洗过程费时费力。In the experiments of molecular biology, microbiology, immunology and other disciplines, the sterility of equipment and equipment is required to be high. Among them, instruments that are used in large quantities and are reused, such as pipettes, require strict cleaning, Disinfection and sterilization operations. The general cleaning process is to wash with detergent first, then soak in acid for 12 hours, then rinse with tap water 15 times, pure water 10 times, ultrapure water 5 times, then put it in a constant temperature oven to dry, and finally autoclave sterilization . The entire operation process is complicated and the procedures are cumbersome, especially the flushing process of tap water, pure water and ultrapure water is time-consuming and laborious.
目前也有一些已公开的清洗装置,以下为部分现有技术的专利文献。There are also some disclosed cleaning devices at present, and the following are some patent documents of the prior art.
专利文献1:授权公告号CN 201454902 U。Patent document 1: Authorized announcement number CN 201454902 U.
专利文献2:授权公告号CN 204583816 U。Patent document 2: Authorized announcement number CN 204583816 U.
专利文献3:授权公告号CN 201283352 Y。Patent document 3: Authorized announcement number CN 201283352 Y.
专利文献4:授权公告号CN 204503709 U。Patent document 4: Authorized announcement number CN 204503709 U.
如上,专利文献1:全自动吸管清洗器,使用单片机控制三种水管的阀门,对不同种类水采用程式化控制,实现冲洗自动化,操作简便,但所清洗的吸管是批量、无序堆放在容器中的,吸管易破损且不能保证清洗的清洁度。As above, Patent Document 1: Fully automatic straw cleaning device, which uses a single-chip microcomputer to control the valves of three water pipes, and uses programmed control for different types of water to realize automatic flushing and easy operation, but the straws to be cleaned are batched and stacked in containers in disorder. Medium, the straw is fragile and cannot guarantee the cleanliness of cleaning.
专利文献2:一种实验室玻璃仪器清洗装置,使用步进电机控制连接有仪器夹具的连接杆转动,转至螺旋喷头处,喷头向上喷水清洗,同时上方有喷淋系统,可同时清洗多个仪器,且各仪器独立清洗、上下喷淋、内外清洁,但各分系统配置分散,设备和水的利用率不高。Patent Document 2: A cleaning device for laboratory glass instruments, which uses a stepping motor to control the rotation of the connecting rod connected to the instrument fixture, and then turns to the spiral nozzle, which sprays water upwards for cleaning, and at the same time, there is a spray system on the top, which can clean multiple at the same time Each instrument is independently cleaned, sprayed up and down, and cleaned internally and externally, but the configuration of each subsystem is scattered, and the utilization rate of equipment and water is not high.
专利文献3:吸管清洗器,在箱体中设置支架放置吸管,并在吸管轴向设置溢流管,通过喷射下方混合的清洗液冲洗吸管,但各吸管仍同在一个容器中,共浸于液体中易发生交叉污染。Patent Document 3: Straw cleaning device, a bracket is set in the box to place the straws, and an overflow pipe is installed in the axial direction of the straws, and the straws are rinsed by spraying the cleaning solution mixed below, but each straw is still in the same container and co-immersed in the Cross-contamination can easily occur in liquids.
专利文献4:一种移液管的清洗装置,成组设置支架,使用蠕动泵将清洗液自上向下灌入移液管内进行清洗,清洗液从移液管下端流出后可收集至清洗液罐内,或收集至其他装置,此装置只能对移液管内部进行清洗。Patent Document 4: A cleaning device for pipettes. Supports are arranged in groups, and a peristaltic pump is used to pour cleaning liquid into the pipette from top to bottom for cleaning. The cleaning liquid flows out from the lower end of the pipette and can be collected into the cleaning liquid. tank, or collected into other devices, this device can only clean the inside of the pipette.
以上文献所述方案,如加装单片机等程控方式实现自动切换水源,如设置支架可以对移液管逐个清洗,如设计水路及加压装置、喷洒结构实现自动清洗,均解决了仪器清洗过程中的部分问题,但仍存在操作不方便、清洁度不足、水利用率低等问题。The solutions described in the above documents, such as adding a single-chip microcomputer and other program-controlled methods to realize automatic switching of water sources, such as setting up brackets to clean the pipettes one by one, such as designing water channels, pressurizing devices, and spraying structures to realize automatic cleaning, all solve the problems in the instrument cleaning process. However, there are still problems such as inconvenient operation, insufficient cleanliness, and low water utilization rate.
发明内容Contents of the invention
为解决上述问题,本发明设计了移液管清洗装置,所述装置具有分流结构,可将用于冲洗的水流分配到各分流管中,并采用形似试管的清洗管用于放置移液管,分流管向清洗管喷射水流对移液管内部进行清洗,水流在清洗管内回流完成对移液管外部的冲洗。清洗管上部设置溢流管将水排出,溢流管汇聚成到出水总管,将水排出。本发明所述移液管清洗装置结构简单、操作方便,所述清洗管具有回流功能,可对移液管内外部进行清洗,提高了移液管清洗的清洁度,同时提高了水的利用率。In order to solve the above problems, the present invention designs a pipette cleaning device, which has a shunt structure, which can distribute the water flow for flushing to each shunt pipe, and adopts a cleaning pipe similar to a test tube for placing the pipette, and the shunt The pipette sprays water to the cleaning tube to clean the inside of the pipette, and the water flows back in the cleaning tube to complete the rinsing of the outside of the pipette. An overflow pipe is arranged on the upper part of the cleaning pipe to discharge the water, and the overflow pipes are converged into the water outlet main pipe to discharge the water. The pipette cleaning device of the present invention is simple in structure and easy to operate. The cleaning pipe has a backflow function and can clean the inside and outside of the pipette, thereby improving the cleanliness of the pipette cleaning and improving the water utilization rate at the same time.
本发明解决技术问题所采用的技术方案是:包括进水喷水装置、清洗出水装置、升降装置;进水喷水装置与升降装置相连,升降装置与清洗出水装置相连;The technical solution adopted by the present invention to solve the technical problem is: comprising a water inlet spray device, a cleaning water outlet device, and a lifting device; the water inlet spraying device is connected with the lifting device, and the lifting device is connected with the cleaning water outlet device;
所述进水喷水装置包括:四通、进水管、进水阀门、总阀门、分流器、分流管、分流阀门、喷嘴;进水管与四通相连,进水管上设有进水阀门,四通与分流器相连,四通与分流器间设有总阀门,分流器与分流管相连,分流管上装有分流阀门,分流管末端装有喷嘴;The water inlet spraying device includes: four-way, water inlet pipe, water inlet valve, main valve, flow divider, shunt pipe, shunt valve, nozzle; The channel is connected with the diverter, the main valve is arranged between the four-way and the diverter, the diverter is connected with the diverter pipe, the diverter valve is installed on the diverter pipe, and the nozzle is installed at the end of the diverter pipe;
所述清洗出水装置包括:清洗管、溢流管、出水总管;清洗管通过溢流管与出水总管连通,溢流管连接在清洗管上口靠下位置;出水总管末端装有出水接头;清洗管具有圆柱形管体,顶部开口,底部封闭,清洗管内部靠上位置装有定位弹片、靠下位置装有V型筛网;清洗管底部装有支撑杆与出水总管连接;The cleaning water outlet device includes: a cleaning pipe, an overflow pipe, and a water outlet main pipe; the cleaning pipe is connected with the water outlet main pipe through the overflow pipe, and the overflow pipe is connected to the position lower than the upper opening of the cleaning pipe; the end of the water outlet main pipe is equipped with a water outlet joint; The pipe has a cylindrical pipe body with an open top and a closed bottom. The upper part of the cleaning pipe is equipped with positioning shrapnel and the lower part is equipped with a V-shaped screen. The bottom of the cleaning pipe is equipped with a support rod to connect with the water outlet main pipe;
所述升降装置包括:支柱腔、支柱、动力腔、电动机、齿轮;支柱顶端与分流器底端固定连接,支柱插在支柱腔内,支柱腔与出水总管的顶端固定连接;动力腔位于支柱腔的一侧,动力腔内装有电动机;支柱靠近电动机一侧设置有齿条,电动机的动力输出轴通过传动装置与齿轮连接,齿轮与齿条啮合。The lifting device includes: a pillar chamber, a pillar, a power chamber, a motor, and a gear; the top of the pillar is fixedly connected to the bottom of the diverter, the pillar is inserted into the pillar chamber, and the pillar chamber is fixedly connected to the top of the outlet main pipe; the power chamber is located in the pillar chamber A motor is installed in the power chamber; a rack is provided on the side of the pillar close to the motor, and the power output shaft of the motor is connected to the gear through the transmission device, and the gear and the rack are meshed.
进一步地,所述分流管以分流器中轴线为中心呈辐射状排列。Further, the splitter tubes are arranged radially with the center axis of the splitter as the center.
进一步地,所述清洗管以出水总管中轴线为中心呈辐射状排列。Further, the cleaning pipes are radially arranged around the central axis of the water outlet main pipe.
进一步地,所述分流管竖直部分和喷嘴的中轴线与清洗管的中轴线重合。Further, the central axis of the vertical part of the distribution pipe and the nozzle coincides with the central axis of the cleaning pipe.
进一步地,所述四通或进水阀门可替换为多路阀或多通管件。Further, the four-way or water inlet valve can be replaced by a multi-way valve or a multi-way pipe fitting.
进一步地,所述清洗管的内径大于移液管外径,优选清洗管的内径等于移液管外径的2-3倍,以节约用水。Further, the inner diameter of the cleaning tube is greater than the outer diameter of the pipette, preferably the inner diameter of the cleaning tube is equal to 2-3 times the outer diameter of the pipette to save water.
进一步地,所述V型筛网由滤片和十字筛芯组成,十字筛芯位于V型筛网中心,滤片自十字筛芯向外呈辐射状排列,滤片竖向放置,利于水流通过。Further, the V-shaped screen is composed of a filter piece and a cross screen core, the cross screen core is located in the center of the V-shaped screen, the filter pieces are arranged radially from the cross screen core, and the filter piece is placed vertically to facilitate the passage of water. .
进一步地,在本发明一个实施例中,所述分流管和清洗管的数量均设置为12支。Further, in an embodiment of the present invention, the number of the shunt pipes and cleaning pipes is set to 12.
进一步地,在本发明一个实施例中,所述清洗管内定位弹片的数量设置为3-6个。Further, in one embodiment of the present invention, the number of positioning elastic pieces in the cleaning tube is set to 3-6.
进一步地,所述进水喷水装置和清洗出水装置中的所有构件均使用耐酸材料制造。Further, all components in the water inlet spraying device and the cleaning water outlet device are made of acid-resistant materials.
本发明的有益效果是,所述移液管清洗装置结构简单、操作方便,所述清洗管具有回流功能,可对移液管内外部进行清洗,提高了移液管清洗的清洁度,同时提高了水的利用率。The beneficial effect of the present invention is that the pipette cleaning device has a simple structure and is easy to operate, and the cleaning pipe has a backflow function, which can clean the inside and outside of the pipette, thereby improving the cleanliness of the pipette cleaning, and at the same time improving the water utilization.
附图说明Description of drawings
下面结合附图和实施例对本发明进一步说明。The present invention will be further described below in conjunction with the accompanying drawings and embodiments.
图1是本发明实施例的剖面结构图;Fig. 1 is the sectional structure drawing of the embodiment of the present invention;
图2是本发明实施例升降装置的剖面结构图;Fig. 2 is a cross-sectional structure diagram of a lifting device according to an embodiment of the present invention;
图3是本发明实施例清洗管排布的俯视图;3 is a top view of the arrangement of cleaning pipes according to an embodiment of the present invention;
图4是本发明实施例V型筛网的俯视结构图;Fig. 4 is a top view structure diagram of a V-shaped screen according to an embodiment of the present invention;
图5是本发明实施例定位弹片排布的俯视图;Fig. 5 is a top view of the arrangement of the positioning shrapnel according to the embodiment of the present invention;
其中,in,
1、进水管;2、进水阀门;3、四通;4、总阀门;5、分流器;6、分流阀门;7、分流管;8、喷嘴;9、定位弹片;10、移液管;11、清洗管;12、V型筛网;1201、滤片;1202、十字筛芯;13、出水总管;14、出水接头;15、升降装置;1501、支柱;1502、支柱腔;1503、齿轮;1504、传动装置;1505、电动机;1506、动力腔;1507、齿条;16、溢流管;17、支撑杆。1. Inlet pipe; 2. Inlet valve; 3. Four-way; 4. Main valve; 5. Splitter; 6. Diverter valve; 7. Diverter pipe; 8. Nozzle; 9. Positioning shrapnel; 10. Pipette ; 11, cleaning pipe; 12, V-shaped screen; 1201, filter plate; 1202, cross screen core; 13, main water outlet; 14, water outlet joint; 15, lifting device; Gear; 1504, transmission device; 1505, motor; 1506, power cavity; 1507, rack; 16, overflow pipe; 17, support rod.
具体实施方式detailed description
首先将实施例连接到实验室环境中。如图1所示,关闭进水阀门2、总阀门4、分流阀门6,分别将自来水、纯水、超纯水的水源连接到三个进水管1上,将出水接头14连接到实验室排水系统上,连接好电动机1505的电源。First connect the example to a lab environment. As shown in Figure 1, close the water inlet valve 2, the main valve 4, and the diversion valve 6, respectively connect the water sources of tap water, pure water, and ultrapure water to the three water inlet pipes 1, and connect the water outlet connector 14 to the laboratory drainage On the system, connect the power supply of the motor 1505.
做好清洗前的准备。启动电动机1505,将进水喷水装置提升,将待清洗的移液管10分别放置在各清洗管11中。定位弹片9和V型筛网12可将移液管10限定在清洗管11的正中央。将升降装置15降落至合适的高度。Prepare for cleaning. Start the motor 1505, lift the water spraying device, and place the pipettes 10 to be cleaned in the cleaning pipes 11 respectively. The positioning shrapnel 9 and the V-shaped screen 12 can limit the pipette 10 to the center of the cleaning tube 11 . Lower the lifting device 15 to a suitable height.
开始清洗。每支分流管7上均设有分流阀门6,当需清洗的仪器数量少于清洗管11数量时,可关闭部分分流管7,以节约用水。打开分流阀门6、总阀门4,并按照清洗的要求,分别打开控制自来水、纯水、超纯水的进水阀门2,将水流经四通3、分流器5、分流管7送至喷嘴8处,水流经喷嘴8喷射到移液管10内部。水流经移液管10尖端、V型筛网流出,并在清洗管11底部回流上升,对移液管10的外部进行清洗。当水充盈至溢流管16位置时,可将水经溢流管16汇聚至出水总管13排出。Start cleaning. Each shunt pipe 7 is provided with a shunt valve 6, and when the number of instruments to be cleaned is less than the number of cleaning pipes 11, part of the shunt pipe 7 can be closed to save water. Open the diversion valve 6 and the main valve 4, and according to the cleaning requirements, respectively open the water inlet valve 2 for controlling tap water, pure water and ultrapure water, and send the water to the nozzle 8 through the four-way 3, the diverter 5 and the diverter pipe 7 , water flows through the nozzle 8 and sprays into the inside of the pipette 10 . The water flows through the tip of the pipette 10 , flows out through the V-shaped screen, and flows back up at the bottom of the cleaning pipe 11 to clean the outside of the pipette 10 . When the water is filled to the position of the overflow pipe 16, the water can be gathered to the water outlet main pipe 13 to be discharged through the overflow pipe 16.
根据不同的清洁度要求,切换进水阀门2以控制自来水、纯水、超纯水的使用时间。According to different cleanliness requirements, switch the water inlet valve 2 to control the use time of tap water, pure water and ultrapure water.
清洗结束。关闭进水阀门2、总阀门4,启动升降装置15,将移液管10取出。Cleaning is over. Close the water inlet valve 2 and the main valve 4, start the lifting device 15, and take out the pipette 10.
当四通3由多通管件代替时,还可将清洗液加入到进水系统中对仪器进行清洗。另外,若进水喷水装置和清洗出水装置中的所有构件均使用耐酸材料制造,则本发明所述移液管清洗装置还可以在泡酸过程中使用。When the four-way 3 is replaced by a multi-way pipe fitting, the cleaning solution can also be added to the water inlet system to clean the instrument. In addition, if all components in the water inlet spraying device and the cleaning water outlet device are made of acid-resistant materials, the pipette cleaning device of the present invention can also be used in the acid soaking process.
Claims (6)
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EP3438674B1 (en) * | 2017-08-01 | 2022-06-22 | Euroimmun Medizinische Labordiagnostika AG | Method and device for cleaning pippeting tips |
TWI816777B (en) * | 2018-04-16 | 2023-10-01 | 大陸商南京金斯瑞生物科技有限公司 | System for extracting biomolecules from a sample and related methods |
CN110102533B (en) * | 2019-04-23 | 2024-01-09 | 佛山市妇幼保健院 | Rubber ball inner cavity cleaning and sterilizing device |
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CN204564693U (en) * | 2015-03-31 | 2015-08-19 | 青岛农业大学 | A kind of many pipettes washer |
JP2015167898A (en) * | 2014-03-06 | 2015-09-28 | 株式会社興洋 | Method and device for cleaning flexible container bag |
CN205463470U (en) * | 2016-03-10 | 2016-08-17 | 内蒙古民族大学 | Liquid transferring tube cleaner |
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US20050000550A1 (en) * | 2003-07-01 | 2005-01-06 | Tyson Fresh Meats, Inc. | Method for backflushing injector needles |
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CN87202818U (en) * | 1987-02-24 | 1988-02-17 | 董庆书 | Automatic pipet flusher |
CN2049560U (en) * | 1988-11-12 | 1989-12-20 | 中国科学院水生生物研究所 | Automatic cleaning apparatus for pipette |
CN202447342U (en) * | 2012-03-05 | 2012-09-26 | 谭曜 | Washing and drying two-functional device of transfer pipette and scale suction pipe |
JP2015167898A (en) * | 2014-03-06 | 2015-09-28 | 株式会社興洋 | Method and device for cleaning flexible container bag |
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