CN105664225A - Nano-silver, chitosan and fibroin compound biological dressing and preparation method thereof - Google Patents
Nano-silver, chitosan and fibroin compound biological dressing and preparation method thereof Download PDFInfo
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- CN105664225A CN105664225A CN201610100176.3A CN201610100176A CN105664225A CN 105664225 A CN105664225 A CN 105664225A CN 201610100176 A CN201610100176 A CN 201610100176A CN 105664225 A CN105664225 A CN 105664225A
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- 229920001661 Chitosan Polymers 0.000 title claims abstract description 63
- 108010022355 Fibroins Proteins 0.000 title claims abstract description 61
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 150000001875 compounds Chemical class 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 239000004332 silver Substances 0.000 claims abstract description 32
- 229910052709 silver Inorganic materials 0.000 claims abstract description 32
- BDAGIHXWWSANSR-UHFFFAOYSA-N Formic acid Chemical compound OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 11
- 239000002245 particle Substances 0.000 claims abstract description 9
- 235000019253 formic acid Nutrition 0.000 claims abstract description 7
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract description 5
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 26
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 13
- 239000002121 nanofiber Substances 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 238000009987 spinning Methods 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 239000005030 aluminium foil Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 claims description 4
- 230000006196 deacetylation Effects 0.000 claims description 2
- 238000003381 deacetylation reaction Methods 0.000 claims description 2
- 239000004744 fabric Substances 0.000 claims description 2
- 238000007540 photo-reduction reaction Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- -1 silver ions Chemical class 0.000 abstract description 4
- 230000001954 sterilising effect Effects 0.000 abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 230000036592 analgesia Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 230000023597 hemostasis Effects 0.000 abstract description 3
- 230000035699 permeability Effects 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000001257 hydrogen Substances 0.000 abstract description 2
- 229910052739 hydrogen Inorganic materials 0.000 abstract description 2
- 150000002500 ions Chemical class 0.000 abstract description 2
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 238000004132 cross linking Methods 0.000 abstract 1
- 239000002861 polymer material Substances 0.000 abstract 1
- 230000001172 regenerating effect Effects 0.000 abstract 1
- 206010052428 Wound Diseases 0.000 description 23
- 208000027418 Wounds and injury Diseases 0.000 description 23
- 102000008186 Collagen Human genes 0.000 description 8
- 108010035532 Collagen Proteins 0.000 description 8
- 229920001436 collagen Polymers 0.000 description 7
- 239000000835 fiber Substances 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 230000029663 wound healing Effects 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 230000000845 anti-microbial effect Effects 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 229920000742 Cotton Polymers 0.000 description 2
- 206010039509 Scab Diseases 0.000 description 2
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 description 2
- 230000000202 analgesic effect Effects 0.000 description 2
- 229940030225 antihemorrhagics Drugs 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000000025 haemostatic effect Effects 0.000 description 2
- 208000014674 injury Diseases 0.000 description 2
- 230000009545 invasion Effects 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000006722 reduction reaction Methods 0.000 description 2
- 230000008591 skin barrier function Effects 0.000 description 2
- 239000004753 textile Substances 0.000 description 2
- 230000008733 trauma Effects 0.000 description 2
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000008946 Fibrinogen Human genes 0.000 description 1
- 108010049003 Fibrinogen Proteins 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 206010048038 Wound infection Diseases 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 238000012824 chemical production Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 210000000416 exudates and transudate Anatomy 0.000 description 1
- 229940012952 fibrinogen Drugs 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 208000026278 immune system disease Diseases 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 239000011505 plaster Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000036560 skin regeneration Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/44—Medicaments
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
- A61L2300/104—Silver, e.g. silver sulfadiazine
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Epidemiology (AREA)
- Hematology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Inorganic Chemistry (AREA)
- Textile Engineering (AREA)
- Materials For Medical Uses (AREA)
Abstract
The invention relates to a nano-silver, chitosan and fibroin compound biological dressing and a preparation method thereof. Natural high-polymer materials of chitosan and fibroin are dissolved by methanoic acid to prepare solutions in different concentrations; the solutions are mixed according to a certain mass ratio; then, silver ions are mixed into the chitosan/fibroin compound biological dressing through electrostatic spinning; next, ultraviolet light is used for irradiation, so that the sliver ions are reduced into nano-silver particles; finally, the prepared compound biological dressing is put into glutaraldehyde stem for sealed crosslinking. Hydrogen bonds among molecules have a strong effect, so that the fibroin and the chitosan have good miscibility; the nano-silver with a good antibiosis effect is combined, so that the nano-silver/chitosan/fibroin compound biological dressing has the advantages that good water absorption and permeability, fast hemostasis capability and analgesia are realized; the wound surface heeling is promoted; the compound biological dressing can continuously act on regenerative bacteria; durable and efficient sterilization characteristics are realized.
Description
Technical field
The invention belongs to the preparation field of biological textiles, in particular to a kind of nanometer silver, chitosan and fibroin compound bio dressing and its preparation method.
Background technology
Skin is the natural cover for defense of human body, to maintaining the stable of internal milieu and stop microorganism invasion to play an important role. If skin is subject to big area damage, then can cause many local even systemic problems, as metabolism aggravation, moisture and protein excessively scatter and disappear, immune system disorder etc., serious goes back entail dangers to life. Rebuilding or recovering skin barrier function is the ultimate aim burning trauma care, before reaching this target, the Wound dressing of an excellent property can temporarily play the part function of skin, an environment being conducive to wound healing is provided, waits surface of a wound epithelization or be transitioned into and rebuild permanent skin barrier.
Wound dressing can be divided into traditional dressing, biological dressing and synthetic dressing etc. according to its material. The most often use and be soaked with the cotton gauze of liquid, apply ointment or plaster on wound or embed in wound, play hemostasis, analgesia, anti-inflammatory, maintenance wound humidity environment, impel wound healing etc. to act on. Traditional cotton gauze liquid absorption is less, 4～5 times of own wt usually only, simultaneously the fiber of gauze coarse cannot with tissue compatible, easily cause wound cambium to damage when changing dressings, extend wound healing time. Compared with traditional weaving medical dressing, biological dressing, owing to having the features such as good degradability, biocompatibility and toxic side effect are little, has unique effect in extensive wound is treated, becomes the focus of textile science and biomedical research. At present, the materials such as the multiplex collagen of biological dressing, chitosan, Lalgine, hyaluronic acid, based on forms such as film, sponge, gels. Although at present the biological dressing of exploitation is functionally constantly perfect, but all existing defects, cannot really meet clinical needs.
There are some defects in the pure collagen dressing in collagen class dressing: collagen has certain antigenicity in physiological conditions; Collagem membrane is easily dry and cracked, it may also be useful to time application gauze dressing cover it keep the moistening of the surface of a wound; And the degradation rate of collagen is too fast, physical strength is little. Yet there are some shortcomings at present in compound collagen biological dressing, as skin can not be replaced to become permanent coverture; Except autologous skin, other all cannot eliminate its antigenicity completely; Make, preserve, transplant costly etc., thus limit their widespread use to a certain extent. In non-collagen class dressing, Lalgine dressing can be used for protection and wound repairing, treatment burn. But alginates dressing should not be used for drying or have the surface of a wound of hard scab. The ventilative aspect of the selectivity of chitosan dressing also needs to improve further, and chemical production chitosan easily pollutes, production cost height, expensive, thus limits it to a certain extent and use. The water-absorbent of fibroin dressing, toughness, mechanicalness and anti-microbial property are poor, cover so fibroin membrane is seldom used for separately the surface of a wound, but make fibroin blend film by blended to itself and some macromolecular materials, to improve its performance.
The ideal structure of wound dressing has high porosity and good barrier. So far, nano fibrous membrane shows good application potential quality in art of wound dressings. Utilize nano fibrous membrane can meet a lot of requirement as high gas permeability as wound dressing, tunica fibrosa evenly can be combined closely with wound surface can not form ponding, and wound infection and dehydration can be prevented, crust synthesis speed can be accelerated and can promote that dermal tissue is shaped, be the good material of trauma care.
Summary of the invention
For solving the problems of the technologies described above, it is an object of the invention to provide one to there is good water suction and water permeable ability, quick-acting haemostatic powder ability, analgesic activity, wound healing, act on regenerated bacteria continuously, there is nanometer silver lasting, sterilization feature efficiently, chitosan and fibroin compound bio dressing and its preparation method.
The nanometer silver of the present invention, chitosan and fibroin compound bio dressing, it is made up of chitosan and the fibroin nanofiber containing nanometer silver, the mass ratio of described chitosan and described fibroin is 1:20～1:5, and described nanometer silver is uniformly distributed in the surface of described nanofiber.
Further, the described chitosan containing nanometer silver and fibroin nanofiber diameter are distributed as 30～200nm.
Further, the diameter of described nano-Ag particles is 5～50nm.
The preparation method of the nanometer silver of the present invention, chitosan and fibroin compound bio dressing, comprises step:
(1) prepare chitosan solution: be dissolved in the formic acid that concentration is 98wt% by the chitosan that deacetylation is 80%, be stirred to and dissolve completely, obtain the chitosan solution that concentration is 1wt%～3wt%;
(2) prepare fibroin solution: be dissolved in the formic acid that concentration is 98wt% by the fibroin that purity is 100%, be stirred to and dissolve completely, obtain the fibroin solution that concentration is 15wt%～30wt%;
(3) chitosan/fibroin/silver nitrate solution is prepared: get chitosan solution and fibroin solution, it is that 1:20～1:5 mixes by chitosan and the mass ratio of fibroin, stir after evenly and add silver nitrate powder, add-on is the 0.5wt%～5wt% of chitosan and fibroin total mass in mixing solutions, continue stirring 0.5～2 hour, until Silver Nitrate dissolves completely;
(4) chitosan/fibroin/silver nitrate solution obtained is carried out electrostatic spinning, with aluminium foil or fabrics accept nano thread, form nano fibrous membrane;
(5) UV-irradiation is adopted to carry out photoreduction in 2～3 hours the nano fibrous membrane obtained;
(6) nanometer silver/chitosan/fibroin compound bio dressing of preparation in described step (5) is placed in glutaraldehyde steam and carries out sealing crosslinked 1～3 hour.
Further, in described step (4), electrostatic spinning process parameter is: voltage is 18～35KV, and reception distance is 8～22cm, and spinning speed is 0.5ml/h, and orifice internal diameter 0.5mm, spinning at room temperature carries out.
By such scheme, the present invention at least has the following advantages:
1, chitosan has biological activity and anastalsis, the synthesis of the formation of fibrinogen element, the hyperplasia of phoirocyte and collagen protein can be stoped, the Wound dressing being made up of chitosan has good biocompatibility, degradability and ventilation property, also has the protection surface of a wound for big area burn and scald, absorbs the effects such as wound exudate, anti-inflammation and sterilization, hemostasis, analgesia, promotion wound healing and skin regeneration;
2, after fibroin dressing flap coverage, effectively avoiding the surface of a wound to expose, stop microorganism invasion, avoid body fluid to run off in a large number, suppression inflammatory cell propagation and the long-pending liquid of the surface of a wound, intelligent adjustment local wound microenvironment, prevents traumatic infection;
3, presoma-the Silver Nitrate of nanometer silver is joined in chitosan/fibroin solution and makes uniform spinning solution, by electrostatic spinning, negatively charged ion is incorporated in fiber, then by UV-irradiation, the silver ion reduction in fiber is become nano-Ag particles, the nano-Ag particles diameter obtained so only several nanometers, and nano-Ag particles is dispersed in fibrefelt, simultaneously owing to nanometer silver and nanofiber are once-forming, nano-Ag particles will be attached to chitosan/fibroin composite nano fiber surface or embed in fiber, can effectively reduce coming off and agglomeration of nanometer silver like this, the nanometer silver of formation is more uniformly distributed in compound bio dressing, silver has good antibacterial and security and stability, the nano-Ag particles made with nanotechnology form has the incomparable broad-spectrum antimicrobial of traditional antiseptic-germicide, strong bactericidal, security height, the advantage such as have no drug resistance, have a wide range of applications at antibacterial field of medicaments,
4, the nanofiber diameter adopting electrostatic spinning to produce is 5～500nm, compare spinning prepared by traditional method, little 2～4 orders of magnitude of electrostatic spinning diameter, electrospun fibers surface-area is big, porosity height, be conducive to cell adhesion and propagation, ventilative, water vapour permeability is good, is that reparation and the healing of wound provides good environment;
5, due to the strong effect of intermolecular hydrogen bonding, fibroin and chitosan have good can Combination, by promoting, albumen synthesis activates inoblast to chitosan, again in conjunction with the nanometer silver of strong anti-microbial effect, nanometer silver/chitosan/fibroin compound bio dressing has good water suction and water permeable ability, quick-acting haemostatic powder ability, analgesic activity, wound healing, acts on regenerated bacteria continuously, has sterilization feature lasting, efficient.
Above-mentioned explanation is only the general introduction of technical solution of the present invention, in order to better understand the technique means of the present invention, and can be implemented according to the content of specification sheets, below with the better embodiment of the present invention and coordinate accompanying drawing to be described in detail as follows.
Accompanying drawing explanation
Fig. 1 and Fig. 2 is the scanning electron microscope (SEM) photograph of the obtained nanometer silver/chitosan/fibroin compound bio dressing of embodiment 1;
Fig. 3 is the scanning electron microscope (SEM) photograph of the obtained nanometer silver/chitosan/fibroin compound bio dressing of embodiment 2.
Embodiment
Below in conjunction with drawings and Examples, the specific embodiment of the present invention is described in further detail. Following examples are for illustration of the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
Take 0.2 gram of chitosan and 1.5 grams of fibroins are dissolved in the formic acid solution that 10 ml concns are 98wt% respectively, it is stirred to chitosan under normal temperature after sealing and fibroin dissolves all completely. Stir evenly after getting 5 grams of chitosan solutions and 10 grams of fibroin solution mixing, then 0.03 gram of silver nitrate powder is added, continue after sealing to stir 0.5 hour, light yellow clear liquid is formed after Silver Nitrate dissolves completely, leave standstill and carry out electrostatic spinning after 30 minutes, voltage is 30kV, syringe pump fltting speed is 0.4ml/h, employing aluminium foil receives, receiving distance is 15 centimetres, orifice internal diameter 0.5mm, spinning at room temperature carries out, and obtains electrostatic and spins nanometer silver/chitosan/fibroin compound bio dressing and carry out sealing in glutaraldehyde steam crosslinked 2 hours. As depicted in figs. 1 and 2, wherein average fibre diameter is 125nm to finished product electron scanning micrograph, and the median size of Argent grain is 13nm.
Embodiment 2
Take 0.2 gram of chitosan and 1.5 grams of fibroins are dissolved in the formic acid solution that 10 ml concns are 98wt% respectively, it is stirred to chitosan under normal temperature after sealing and fibroin dissolves all completely. Stir evenly after getting 5 grams of chitosan solutions and 10 grams of fibroin solution mixing, then 0.06 gram of silver nitrate powder is added, continue after sealing to stir 0.5 hour, light yellow clear liquid is formed after Silver Nitrate dissolves completely, leave standstill and carry out electrostatic spinning after 30 minutes, voltage is 30kV, syringe pump fltting speed is 0.4ml/h, employing aluminium foil receives, receiving distance is 15 centimetres, orifice internal diameter 0.5mm, spinning at room temperature carries out, and obtains electrostatic and spins nanometer silver/chitosan/fibroin compound bio dressing and carry out sealing in glutaraldehyde steam crosslinked 3 hours. As shown in Figure 3, wherein average fibre diameter is 101nm to finished product electron scanning micrograph, and the median size of Argent grain is 32nm.
Nanometer silver/the chitosan of the present invention/fibroin compound bio dressing is taking natural macromolecular material chitosan and fibroin mixture as base material, by electrostatic spinning, silver ions is incorporated in chitosan/fibroin compound bio dressing, then silver ion reduction is made to become nano-Ag particles by UV-irradiation, it is to increase the anti-microbial property of compound bio dressing.
The above is only the preferred embodiment of the present invention; it is not limited to the present invention; should be understood that; for those skilled in the art; under the prerequisite not departing from the technology of the present invention principle; can also making some improvement and modification, these improve and modification also should be considered as protection scope of the present invention.
Claims (5)
1. a nanometer silver, chitosan and fibroin compound bio dressing, it is characterized in that: be made up of chitosan and the fibroin nanofiber containing nanometer silver, the mass ratio of described chitosan and described fibroin is 1:20～1:5, and described nanometer silver is uniformly distributed in the surface of described nanofiber.
2. nanometer silver according to claim 1, chitosan and fibroin compound bio dressing, it is characterised in that: the described chitosan containing nanometer silver and fibroin nanofiber diameter are distributed as 30～200nm.
3. nanometer silver according to claim 1, chitosan and fibroin compound bio dressing, it is characterised in that: the diameter of described nano-Ag particles is 5～50nm.
4. the preparation method of a nanometer silver, chitosan and fibroin compound bio dressing, it is characterised in that comprise step:
(1) prepare chitosan solution: be dissolved in the formic acid that concentration is 98wt% by the chitosan that deacetylation is 80%, be stirred to and dissolve completely, obtain the chitosan solution that concentration is 1wt%～3wt%;
(2) prepare fibroin solution: be dissolved in the formic acid that concentration is 98wt% by the fibroin that purity is 100%, be stirred to and dissolve completely, obtain the fibroin solution that concentration is 15wt%～30wt%;
(3) chitosan/fibroin/silver nitrate solution is prepared: get chitosan solution and fibroin solution, it is that 1:20～1:5 mixes by chitosan and the mass ratio of fibroin, stir after evenly and add silver nitrate powder, add-on is the 0.5wt%～5wt% of chitosan and fibroin total mass in mixing solutions, continue stirring 0.5～2 hour, until Silver Nitrate dissolves completely;
(4) chitosan/fibroin/silver nitrate solution obtained is carried out electrostatic spinning, with aluminium foil or fabrics accept nano thread, form nano fibrous membrane;
(5) UV-irradiation is adopted to carry out photoreduction in 2～3 hours the nano fibrous membrane obtained;
(6) nanometer silver/chitosan/fibroin compound bio dressing of preparation in described step (5) is placed in glutaraldehyde steam and carries out sealing crosslinked 1～3 hour.
5. the preparation method of nanometer silver according to claim 4, chitosan and fibroin compound bio dressing, it is characterized in that: in described step (4), electrostatic spinning process parameter is: voltage is 18～35KV, reception distance is 8～22cm, spinning speed is 0.5ml/h, orifice internal diameter 0.5mm, spinning at room temperature carries out.
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