CN105662940B - Active compound and preparation method thereof and its application in cosmetics or skin care item - Google Patents

Abstract

The invention discloses an active composition and a preparation method thereof, and its application in cosmetics or skin care products, wherein the preparation method of the active composition comprises: 50-60 parts by mass of sugar maple sap, 10-60 parts by mass, 30 parts of birch sap and 10-20 parts of coconut water are mixed to form a mixed solution, and then the mixed solution is sequentially subjected to enzyme elimination, clarification, refining, concentration and stabilization to form the active composition. The active composition prepared by the preparation method of the present invention can effectively inhibit the activity of tyrosinase, eliminate free radicals, and have a good antioxidant effect; the active composition is applied to the preparation of cosmetics or skin care products, and the obtained cosmetics or skin care products In addition to the natural and fresh plant smell, the product also has long-lasting anti-oxidation and whitening effects.

Description

Active composition and method for its preparation, and its use in cosmetic or skin care products

technical field

The invention relates to the technical field of daily necessities, in particular to an active composition, a preparation method, and an application in cosmetics or skin care products.

Background technique

Tyrosinase is a key enzyme for melanin synthesis, and its abnormal overexpression can lead to pigmentation diseases in the human body. Therefore, tyrosinase inhibitors can treat the current common pigmentation. At present, most of the tyrosinase inhibitors added to whitening cosmetics on the market are chemical compounds with obvious toxic and side effects. The use of natural source whitening agents to prepare cosmetics is the future development direction of cosmetics. Skin aging is a natural phenomenon of the human body. Nowadays, the problem of environmental pollution is serious, people's work pressure is high, and their lives are irregular, which makes the phenomenon of skin aging more serious. The most common endogenous biochemical factors associated with the aging process are free radicals, so for anti-aging, scavenging free radicals is particularly important.

Therefore, it is of great significance in the development of cosmetics or skin care products to develop plant active compositions with whitening and antioxidant activity effects.

Contents of the invention

In view of the above-mentioned problems in the prior art, the present invention proposes an active composition with whitening and anti-oxidation activities, its preparation method, and its application in cosmetics or skin care products.

To achieve the above object, the present invention adopts the following technical solutions:

The invention discloses a preparation method of an active composition, comprising: mixing 50-60 parts by mass of sugar maple sap, 10-30 parts of white birch sap and 10-20 parts of coconut water to form a mixture liquid, and then the mixed liquid is inactivated, clarified, refined, concentrated and stabilized sequentially to form the active composition.

In a further technical scheme, the preparation method of the active composition disclosed in the present invention can also have the following technical features:

The step of inactivating enzymes includes using microwaves to inactivate biological enzymes on the mixed liquid, wherein the power of the microwaves is 300-900W, and the time is 5-10 minutes.

The clarification step includes using an ice bath to cool down the mixed solution after the enzyme-inactivating treatment to room temperature, and then performing high-speed refrigerated centrifugation, wherein the centrifugation speed is 18000-25000 rpm, the time is 10-15 minutes, and the temperature is 0-2000 rpm. 5°C.

The refining step includes passing the mixed solution after the clarification step through an ultrafiltration membrane with a molecular weight of 3000-5000 Da to collect the permeate; preferably, the material of the ultrafiltration membrane is a hollow fiber membrane, a polyacrylonitrile membrane , polyvinyl chloride membrane, polysulfone membrane, polyethersulfone membrane, polyvinylidene fluoride membrane or ceramic membrane.

The concentrating step includes vacuum film concentrating the mixed solution after the refining step, wherein the concentration ratio is 1/15 to 1/10 of the mass of the mixed solution before the enzyme-inactivating treatment step, and the rotation speed is 700~1000rpm, the vacuum degree is below 100Pa, and the temperature is 10~20℃.

The stabilizing step includes adding a stabilizer to the mixed solution that has gone through the concentration step and mixing evenly, the mass of the stabilizer is 20-40% of the mass of the mixed solution that has gone through the concentration step.

The stabilizer is a mixture of propylene glycol, 1,3-butanediol and 1,2-pentanediol, wherein the mixing mass ratio of propylene glycol, 1,3-butanediol and 1,2-pentanediol is 3- 6: 1~3: 1~2.

The sugar maple sap, birch sap and coconut water are stirred and mixed at a temperature of 0-5° C. to form a mixed solution.

The invention also discloses an active composition, which is the active composition prepared according to the above-mentioned preparation method.

The present invention also discloses an application of the active composition in cosmetics or skin care products, which is characterized in that the active composition is added to the cosmetics or skin care products at a mass fraction of 1-40%.

Sugar maple, also known as: sugar maple, American sugar maple, is a large deciduous tree in the Aceraceae family, with a height of 40m. "National Compilation of Chinese Herbal Medicine" records that the roots and leaves of maple tree can be used as medicine, which can dispel wind and relieve pain. The colorless and easy-flowing solution flowing from the sugar maple trunk is called sugar maple sap, which contains 2 to 3% sugar, amino acids and other mineral salts. Due to the special flavor of sugar maple sap, it is also commonly used to make candied fruit, candy or tobacco flavoring. The sugar maple sap used in the present invention is the effluent from the summer sugar maple trunk.

Birch sap is collected from living birch trees in spring. The freshly collected birch sap is cool and delicious, slightly sweet (healthy and disease-free trees), rich in sugar, acid, trace elements and aromatic substances. According to the determination, birch sap contains 4 kinds of vitamins (VC, VB1, VB2, VH), 11 kinds of fatty acids, 18 kinds of amino acids and 18 kinds of trace elements. These substances play an important role in the life activities of the human body. Often drinking birch sap natural beverage can promote the metabolism of the human body, and can resist vascular sclerosis and fatigue. Birch sap in the present invention is the effluent from the trunk of birch in spring.

Coconut water is a complex natural liquid that exists in the cavity of coconut fruit. It is rich in various nutrients and has a unique flavor and aroma. The main components include acids, alcohols, esters, alkenes, aldehydes, and ketones. class and phenols etc. In the food industry, it can be used to produce a variety of foods, such as coconut fiber fruit, fruit wine, vinegar, ethyl acetate, etc. The annual output of coconut fruit in my country is about 289,000 tons, and the source of coconut water is rich, but there are few research reports on coconut water, and the utilization rate is also very low. When most coconut processing factories process coconuts, only a small amount of coconut water is used. Except for refreshing drinks, most of them are dumped as waste, which not only causes environmental pollution, but also causes a huge waste of resources. Therefore, it is of great economic value to study and apply coconut water to the field of daily chemicals. Coconut water among the present invention is to be taken from the cavity liquid of ripe coconut.

Compared with the prior art, the beneficial effect of the present invention is that: the preparation method of the present invention is prepared by mixing the three plant components of sugar maple sap, birch sap and coconut water to form an active composition, and the active composition can Effectively inhibit tyrosinase activity, eliminate free radicals, and have a good antioxidant effect; the active composition is applied to the preparation of cosmetics or skin care products, and the obtained cosmetics or skin care products not only have a natural and fresh plant smell, but also have Long-lasting anti-oxidant and whitening effect.

In a further solution, the three raw materials used in the active composition of the present invention are all rich in oxidase, and the enzyme structure in the three raw materials is rapidly destroyed by microwave method to lose the activity of the enzyme, avoiding the easy oxidation of active ingredients It will lead to darkening of the color and thus affect the application of the active composition in the terminal cosmetics, ensuring the stability of the active composition; preferably, the mixed solution can effectively remove the raw materials through the use of an ultrafiltration membrane, which is likely to cause product instability and is not easy to be absorbed by the skin. The absorbed macromolecular impurities such as starch, polysaccharide, and protein greatly retain active ingredients such as amino acids, mineral salts, and aromatic small molecules, so that the active composition has a better effect when applied to cosmetics or skin care products.

In a further solution, the active composition is kept at a low temperature during the preparation process, which avoids the browning of the sugar in the raw material at high temperature and affects the appearance, thereby ensuring the appearance of the active composition and avoiding the appearance of cosmetics or skin care products. make an impact.

Description of drawings

Fig. 1 is the fitting figure of active composition in the embodiment 1 of the present invention to free radical scavenging;

Fig. 2 is the fitting figure of active composition in the embodiment of the present invention 2 to tyrosinase inhibitory rate;

Fig. 3 is a comparison chart of melanin reduction after 28 days between the blank sample and the formulation product in Example 5 and Example 6 of the present invention;

Fig. 4 is a comparison chart of the L increase value of the blank sample and the formula products in the fifth and sixth examples of the present invention after 28 days.

Detailed ways

The present invention will be further described below with reference to the accompanying drawings and in combination with preferred embodiments.

The invention provides a method for preparing an active composition. In one embodiment, it comprises 50-60 parts by mass of sugar maple sap, 10-30 parts of birch sap and 10-20 parts of Coconut water is mixed to form a mixed solution, and then the mixed solution is sequentially subjected to enzyme inactivation, clarification, refining, concentration and stabilization to form the active composition.

In some preferred embodiments, under the conditions of the foregoing embodiments, at least one of the following preferred conditions can be adopted:

The step of inactivating enzymes includes using microwaves to inactivate biological enzymes on the mixed liquid, wherein the power of the microwaves is 300-900W, and the time is 5-10 minutes.

The clarification step includes using an ice bath to cool down the mixed solution after the enzyme-inactivating treatment to room temperature, and then performing high-speed refrigerated centrifugation, wherein the centrifugation speed is 18000-25000 rpm, the time is 10-15 minutes, and the temperature is 0-2000 rpm. 5°C.

The refining step includes passing the mixed solution after the clarification step through an ultrafiltration membrane with a molecular weight of 3000-5000 Da to collect the permeate; preferably, the material of the ultrafiltration membrane is a hollow fiber membrane, a polyacrylonitrile membrane , polyvinyl chloride membrane, polysulfone membrane, polyethersulfone membrane, polyvinylidene fluoride membrane or ceramic membrane.

The concentrating step includes vacuum film concentrating the mixed solution after the refining step, wherein the concentration ratio is 1/15 to 1/10 of the mass of the mixed solution before the enzyme-inactivating treatment step, and the rotation speed is 700~1000rpm, the vacuum degree is below 100Pa, and the temperature is 10~20℃.

The stabilizing step includes adding a stabilizer to the mixed solution that has undergone the concentration step and mixing evenly, the quality of the stabilizer is 20-40% of the mass of the mixed solution that has passed the concentration step, preferably, the The stabilizer is a mixture of propylene glycol, 1,3-butanediol, and 1,2-pentanediol, wherein the mixing mass ratio of propylene glycol, 1,3-butanediol, and 1,2-pentanediol is 3-6: 1~3: 1~2.

The sugar maple sap, birch sap and coconut water are stirred and mixed at a temperature of 0-5° C. to form a mixed solution.

The present invention also provides an active composition prepared according to the preparation method of any one of the above embodiments.

The present invention also provides an application of the active composition prepared according to the preparation method of any one of the above embodiments in cosmetics or skin care products, wherein the active composition is added to the cosmetic or in skin care products.

The active composition of the present invention and its preparation method will be further described below with specific examples.

Embodiment one:

An active composition containing sugar maple sap is composed of the following components in parts by weight: 50 parts by weight of sugar maple sap, 20 parts by weight of birch sap, and 10 parts by weight of coconut water. The three raw materials were mixed at a temperature of 2°C, and then the enzyme was inactivated under the condition of a microwave power of 450W and a time of 6 minutes; After centrifugation at 0°C, the centrifuged supernatant passes through an ultrafiltration membrane with a molecular weight of 3500Da to obtain a permeate; the permeate is concentrated to three types of membranes at a rotation speed of 800rpm, a vacuum of 80Pa, and a temperature of 20°C. 1/10 of the initial mass of the raw material mixed solution to obtain a concentrated solution; a stabilizer with a mass fraction of 25% is added to the concentrated solution, and the active composition A of the present invention is obtained after conventional mixing. Its solid content is 16%-20%, and it can be directly added to skin care cosmetics. The stabilizer is a mixture of propylene glycol, 1,3-butanediol, and 1,2-pentanediol, and the mixing ratio is 3:3:1.

The active composition A was tested for its ability to eliminate DPPH free radicals as follows:

1,1-Diphenyl-2-trinitrophenylhydrazine (DPPH) is a stable nitrogen-centered organic radical, and its stability mainly comes from resonance stabilization and the steric hindrance of the three benzene rings, making it sandwiched between Among them, the unpaired electrons on the nitrogen atom cannot play their due electron pairing role. When the free radical scavenger exists, its absorption gradually disappears due to single-electron pairing, and its fading degree is quantitatively related to the number of electrons it accepts. Therefore, a spectrophotometer can be used for rapid quantitative analysis to detect the free radical scavenging situation, so as to evaluate The antioxidant capacity of the samples. Its ability is expressed by clearance rate, and the greater the clearance rate, the stronger its antioxidant capacity.

Sample treatment: The active composition A containing sugar maple sap is a light yellow transparent liquid with a fresh aroma. When performing the DPPH removal test, it is diluted with pure water to the required concentration according to the proportion.

Test method: prepare a DPPH ethanol solution with a concentration of 2×10 ^-4 mol/L with absolute ethanol as a solvent; add 2ml of the sample solution to be tested (active composition A) into the test tube, then add 2ml of the DPPH ethanol solution, and mix well Let it stand for 30 minutes; measure the absorbance at 517nm.

Repeat the test three times for each concentration to ensure the accuracy of the experimental results, and finally take the average value as the fitting data, wherein the calculation formula of the DPPH clearance rate is as follows:

DPPH clearance rate (%)=[1-(EE ₀ )/(FF ₀ )]×100%

In the formula:

E: Absorbance of 2ml sample solution plus 2ml DPPH solution;

E ₀ : the absorbance of 2ml water plus 2ml DPPH solution (sample solvent contrast);

F: Absorbance of 2ml sample solution plus 2ml 95% ethanol (positive control);

F ₀ : absorbance of 2ml water plus 2ml 95% ethanol (positive control solvent control).

The DPPH scavenging rate was calculated according to the above formula. The higher the scavenging rate, the stronger the antioxidant capacity. The experimental results are shown in Table 1.

Table 1

Calculation of half inhibitory rate (IC ₅₀ ):

The _C50 value is defined as the concentration of antioxidant required for 50% clearance. The free radical scavenging was plotted and fitted with different concentrations of the active composition A, the result is shown in Figure 1, and the IC ₅₀ value was calculated by reading. It can be seen from the figure that the _IC50 of the DPPH free radical scavenging rate of the active composition containing sugar maple sap is 2.8%, and has a relatively high antioxidant activity.

Embodiment two:

An active composition containing sugar maple sap is composed of the following components by weight: 58 parts by weight of sugar maple sap; 25 parts by weight of birch sap; and 18 parts by weight of coconut water. The three raw materials were mixed at a temperature of 5°C, and then the enzyme was inactivated under the condition of a microwave power of 800W and a time of 6 minutes; Centrifuge at 3°C, and the supernatant after centrifugation passes through a 5000Da ultrafiltration membrane to obtain the permeate; the passthrough is concentrated into a mixture of three raw materials at a rotation speed of 1000rpm, a vacuum of 30Pa, and a temperature of 15°C. 1/12 of the initial mass to obtain a concentrated solution; the concentrated solution is then added with a stabilizer having a mass fraction of 40%, and after conventional mixing, it becomes the active composition B of the present invention. Its solid content is 16%-20%, and it can be directly added to skin care cosmetics. The stabilizer is a mixture of propylene glycol, 1,3-butanediol and 1,2-pentanediol, and the mixing ratio is 5:3:2.

The active composition B is tested for its ability to inhibit tyrosinase activity as follows:

Recent studies have proved that hyperpigmentation is caused by the activation of melanin-producing enzymes in melanocytes in the epidermis by ultraviolet rays to produce pigments. In the process of melanin production, tyrosinase converts tyrosine into dopa and dopaquinone, which are polymerized into macromolecular melanin through non-enzymatic oxidation. Therefore, by inhibiting the activity of tyrosinase, melanin production can be limited and skin pigmentation can be improved.

Sample treatment: The active composition containing sugar maple sap is a light yellow transparent liquid with a fresh aroma. When performing the tyrosinase activity inhibition test, it is diluted with pure water to the required concentration according to the proportion.

Test method: Prepare PBS solution (pH=6.8, 0.1mol/L), L-tyrosinase solution (tyrosinase activity: 100U/ml). In the sample tube and the sample control tube, respectively add the active composition B in 1ml embodiment two, and the positive and negative control tubes are replaced with phosphate buffer; in the sample tube and the sample control tube, add 0.5ml tyrosinase solution, and the sample control And the negative control tube was replaced with 0.5ml phosphate buffer solution, shaken to make the sample fully mixed, incubated at 37°C for 10min; added tyrosinase, after constant temperature for 10min, measured its absorbance value at 475nm, and compared with arbutin .

Tyrosinase inhibition rate (%)=[1-(GG ₀ )/(HH ₀ )]×100%

In the formula:

G: sample group;

G ₀ : sample control group;

H: positive control;

H ₀ : Positive solvent control.

The tyrosinase inhibition rate was calculated according to the above formula, and the experimental results are shown in Table 2.

Table 2

Calculation of half inhibitory rate (IC ₅₀ ):

The _C50 value was defined as the concentration of anti-inhibitor required for 50% inhibition. The tyrosinase inhibition rate was plotted and fitted with different concentrations of the active composition. The result is shown in Figure 2, and the IC ₅₀ value was calculated by reading. It can be seen from the figure that the IC ₅₀ of the active composition containing sugar maple sap for inhibiting tyrosinase activity is 4.9%, and has a relatively high inhibitory activity for tyrosinase.

Embodiment three:

An active composition containing sugar maple sap is composed of the following components in parts by weight: 60 parts by weight of sugar maple sap, 30 parts by weight of birch sap, and 20 parts by weight of coconut water. After the three raw materials were mixed at 0°C, the enzyme was inactivated under the condition of microwave power of 900W and time of 10 minutes; the treated mixed solution was rapidly cooled to room temperature in an ice bath, and then the rotation speed was 18000rpm/min, and the time was 15 minutes. Centrifuge at a temperature of 5°C, and the supernatant after centrifugation passes through a 3000Da ultrafiltration membrane to obtain a permeate; the passthrough is concentrated into three raw materials at a rotation speed of 900rpm, a vacuum of 50Pa, and a temperature of 10°C. 1/15 of the initial mass of the mixed solution to obtain a concentrated solution; the concentrated solution is added with a stabilizer with a mass fraction of 30%, and after conventional mixing, it becomes the active composition C of the present invention. Its solid content is 16%-20%, and it can be directly added to skin care cosmetics. The stabilizer is a mixture of propylene glycol, 1,3-butanediol, and 1,2-pentanediol, and the mixing ratio is 6:1:2. The ability to eliminate DPPH free radicals and the ability to inhibit tyrosinase activity were tested on the active composition C, and it was found that the active composition C had higher antioxidant activity and higher tyrosinase inhibitory activity.

Embodiment four:

An active composition containing sugar maple sap is composed of the following components in parts by weight: 55 parts by weight of sugar maple sap, 10 parts by weight of birch sap, and 15 parts by weight of coconut water. The three raw materials were mixed at a temperature of 3°C, and then the enzyme was inactivated under the condition of a microwave power of 300W and a time of 5 minutes; Centrifuge at 2°C, and the supernatant after centrifugation passes through a 4000Da ultrafiltration membrane to obtain the permeate; the passthrough is concentrated into a mixture of three raw materials at a rotation speed of 700rpm, a vacuum of 60Pa, and a temperature of 12°C. 1/14 of the initial mass to obtain a concentrated solution; the concentrated solution is then added with a stabilizer with a mass fraction of 20%, and after conventional mixing, it becomes the active composition D of the present invention. Its solid content is 16%-20%, and it can be directly added to skin care cosmetics. The stabilizer is a mixture of propylene glycol, 1,3-butanediol, and 1,2-pentanediol, and the mixing ratio is 4:2:1. The ability to eliminate DPPH free radicals and the ability to inhibit tyrosinase activity were tested on the active composition D respectively, and it was concluded that the active composition D had higher antioxidant activity and higher tyrosinase inhibitory activity.

The application of the active composition of the present invention in cosmetics or skin care products will be further described below with specific examples.

Embodiment five:

In this example, the active composition A prepared in Example 1 was added to the formulation of the emulsion to prepare the emulsion. Wherein the formulation of the emulsion is shown in Table 3.

table 3

Mix the raw materials of phase A, dissolve at 75°C, and add phase B at 75°C, stir, and cool to obtain a whitening emulsion.

Example 6: In this example, the active composition B prepared in Example 2 is added to the formula of lotion to prepare lotion. Wherein the formula of lotion is shown in Table 4.

Table 4

serial number INCI name Add mass fraction/% 1 Sorbitol 1-3 2 1,3-Butanediol 1.4 3 PEG1000 0.5-1 4 polyoxyethylene oleyl ether 1-3 5 ethanol 5-10 6 active composition B 1-40 7 essence 0.01-0.1 8 purified water margin

The raw materials in the above Table 4 were mixed and dissolved, and the lotion was obtained after stirring.

The emulsion prepared in Example 5 and the lotion prepared in Example 6 were evaluated for human body whitening efficacy as follows.

The active composition used in the present invention can be used as a functional raw material for whitening and anti-aging cosmetics. There is no special requirement for its use form, and it can be used in various cosmetics such as water preparations and emulsions. In the human body whitening efficacy experiment of the present application invention, the formula products in Embodiment 5 and Embodiment 6 were used, and the mass ratios of the active composition added were 10% and 30% respectively.

Subject population: It consists of 10 healthy volunteers aged 25-45.

Test method: The test subject uses cosmetics on the inside of the subject's forearm with a marker pen and divides it into two application areas. The subjects smeared the test sample on the inside of the forearm every morning and evening, and smeared a blank formula product (excluding the active composition of the present invention) near the wrist for 28 consecutive days. The melanin value and L value of each area were collected by an instrument, and the average value was calculated.

Data acquisition: MPA580 skin tester from Germany CK Company was used, in which Mexameter MX18 (melanin and hemoglobin test probe) was used to detect skin melanin, and Colorimeter CL400 (skin color test probe) was used to detect skin color uniformity.

Evaluation results: Melanin is a common standard for us to judge the black and white of the skin. The brighter the skin tone, the lower the melanin value. As can be seen from Figure 3, after the two test samples have been used continuously for 28 days, comparing the results of the samples applied on both sides, it can be clearly seen that the amount of melanin reduction in the formula products containing 10% of the active composition and 30% of the active composition is greater than that of the blank. Therefore, applying skin care cosmetics containing 10% and 30% of the active composition can have a good effect on controlling melanin production.

The L value is an indicator that defines color, and in skin color we use it to define the uniform brightness of the skin tone. The more even and bright the skin tone, the higher the L value. It can be seen from Figure 4 that after the two samples were used continuously for 28 days, the L value on the side where the blank sample was applied increased less, so applying skin care products or cosmetics containing 10% and 30% of the active composition has a better effect on improving skin brightness. Effect.

As can be seen from the above examples, the active composition prepared by the preparation method of the present invention can effectively inhibit the activity of tyrosinase, eliminate free radicals, and have good antioxidant effect; the active composition is applied to the preparation of cosmetics Or skin care products, the obtained cosmetics or skin care products not only have a natural and fresh plant smell, but also have long-lasting anti-oxidation and whitening effects.

The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be assumed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art to which the present invention belongs, several equivalent substitutions or obvious modifications can be made without departing from the concept of the present invention, and those with the same performance or use should be deemed to belong to the protection scope of the present invention.

Claims (13)

1. a kind of preparation method of active compound, which is characterized in that including：It is respectively 50 ~ 60 parts of sugar maple by mass fraction Sap, 10 ~ 30 parts of white birch myron and 10 ~ 20 parts of coconut water are mixed to form mixed liquor, then by the mixed liquor successively into Row enzyme deactivation, clarification form the active compound after refining, concentrate and stablizing.

2. preparation method according to claim 1, which is characterized in that the enzyme deactivation step is included using microwave to described mixed It closes liquid and carries out biological enzyme inactivation treatment, wherein the power of the microwave is 300 ~ 900W, the time is 5 ~ 10min.

3. preparation method according to claim 1, which is characterized in that the clarification steps, which include to pass through using ice bath, goes out The mixed liquor after enzymatic treatment is cooled to room temperature, then carries out high speed refrigerated centrifuge, wherein the rate of the centrifugation is 18000 ~ 25000rpm, time are 10 ~ 15min, and temperature is 0 ~ 5 DEG C.

4. preparation method according to claim 1, which is characterized in that the purification step includes will be after clarification steps The mixed liquor pass through molecular weight be 3000 ~ 5000Da ultrafiltration membrane, collect permeate.

5. preparation method according to claim 4, which is characterized in that the material of the ultrafiltration membrane is hollow-fibre membrane.

6. preparation method according to claim 4, which is characterized in that the material of the ultrafiltration membrane is polyacrylonitrile film, gathers Vinyl chloride film, PS membrane, poly (ether sulfone) film, polyvinylidene fluoride film or ceramic membrane.

7. preparation method according to claim 1, which is characterized in that the concentration step includes that purification step will be passed through The mixed liquor carries out vacuum film concentration, and wherein concentration ratio is by the mixed liquor before the destroy the enzyme treatment step Quality 1/15 ~ 1/10, the speed of rotation be 700 ~ 1000rpm, vacuum degree be below 100Pa, temperature be 10 ~ 20 DEG C.

8. preparation method according to claim 1, which is characterized in that the stabilizing step is included to by concentration step Stabilizer is added in the mixed liquor and is uniformly mixed, the quality of the stabilizer is the mixed liquor by concentration step The 20 ~ 40% of quality.

9. preparation method according to claim 8, which is characterized in that the stabilizer is propylene glycol, 1,3-BDO, 1, The mixture of 2- pentanediols, wherein propylene glycol, 1,3-BDO, the mixing quality ratio of 1,2- pentanediol is 3~6：1~3：1 ~2.

10. according to claim 1 to 9 any one of them preparation method, which is characterized in that sugar maple sap, white birch myron and coconut palm Sub- water forms mixed liquor after mixing is stirred at a temperature of 0 ~ 5 DEG C.

11. a kind of active compound, which is characterized in that be made of the preparation method described according to any of claims 1 to 10 Active compound.

12. application of a kind of active compound of claim 11 in cosmetics, which is characterized in that the active compound is pressed It is added to according to the ratio that mass fraction is 1 ~ 40% in the cosmetics.

13. application of a kind of active compound of claim 11 in skin care item, which is characterized in that the active compound is pressed It is added to according to the ratio that mass fraction is 1 ~ 40% in the skin care item.