CN105646070A - Special additive for pleurotu comucopiae culture medium - Google Patents
Special additive for pleurotu comucopiae culture medium Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention relates to the technical field of addition of an edible fungus culture medium, in particular to a special additive for a pleurotu comucopiae culture medium. The special additive is prepared from the following raw materials in parts by weight: 20 to 40 parts of caulis polygoni multiflori, 10 to 20 parts of spica prunellae, 5 to 10 parts of rhizoma atractylodis macrocephalae, 10 to 20 parts of fructus citri sarcodactylis, 5 to 10 parts of pericarpium citri reticulatae viride, 5 to 10 parts of semen cassia, 30 to 50 parts of ginkgo leaf, 10 to 20 parts of Chinese pyrola herb, 5 to 11 parts of ramulus cinnamomi, 10 to 30 pats of cibotium barometz and 6 to 16 parts of uncaria rhynchophylla. When the special additive for the pleurotu comucopiae culture medium, disclosed by the invention, is applied to the pleurotu comucopiae culture medium, the nutrition and the flavor of pleurotu comucopiae can be improved while the growth of the pleurotu comucopiae is promoted, the yield is increased, and the content of heavy metal in the pleurotu comucopiae is remarkably reduced; meanwhile, the antioxidation and anticancer efficacy of the pleurotu comucopiae can be improved.
Description
Technical field
The present invention relates to edible fungi culture medium additive technical field, particularly pocket mushrooms culture medium is specialUse additive.
Background technology
Pocket mushrooms has another name called elegant precious mushroom, yellowish-whitely picks up the ears, handle bucket mushroom and small mushroom etc., be subordinate to Basidiomycetes,Zoopagales, Pleurotaceae, Pleurotus, its fructification list is raw or grow thickly, piece little shape U.S., mushroom lid diameter 1～3cm,There are 2 kinds of light gray and milkys. Mushroom handle is sturdy, long 2～3cm, and middle reality, look even. Pocket mushrooms belongs toNutrition is high, and the healthy food that heat is low, containing crude protein, carbohydrate, fiber, thiamine, coreThe trace elements such as flavine, nicotinic acid, calcium, iron, potassium, sodium, phosphorus, the content of its protein is higher than generallyVegetables. The amino acid contained kind of pocket mushrooms is very abundant, and the wherein content of protein is more domestic than at presentFlat mushroom, agaricus bisporus, pocket mushrooms and the straw mushroom of cultivation are all high, and eight kinds of essential amino acids of human body are completeAll possess, its content accounts for the more than 35% of all total amino acid contents. So often edible elegant precious mushroom, canStrengthen health. According to another studies confirm that recently, some physiological activators that elegant precious mushroom contains, toolHave and bring out the synthetic of interferon, improve immune function of human body, there is anti-cancer, anticancer effect. Elegant preciousMushroom is fatty, starch is little, is diabetes patient and obesity patient's ultimate food, reduces in addition courageThe effect of sterol. People are called " healthy food ", " safety food ".
Pocket mushrooms cap is abundant, and quality is tender and crisp, and delicious flavour quite gets consumer reception, the market demandMeasure increasingly, therefore, cultivated area is increasing. At present, generally all adopt cornstalk, weedtreeBits or cotton seed hulls are that primary raw material is cultivated pocket mushrooms, but conventional plantation raw material resources are fewer and feweri, andNutritional labeling is single, and content is also lower, affects output and the healthy nutritive value of pocket mushrooms, finds newAdditive supplement existing raw material resources, become extremely urgent thing. Meanwhile, pocket mushrooms cultivationBase is infected by miscellaneous bacteria easily, and productive rate is low, and quality is influenced, affects economic benefit.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of pocket mushrooms culture medium specialist additive,When promoting pocket mushrooms growth, can also improve the anti-oxidant and anticancer function of pocket mushrooms.
For achieving the above object, the present invention adopts following technical scheme:
Pocket mushrooms culture medium specialist additive, is made up of the raw material of following weight portion: Polygonum multiflower knotweed 20～40Part, 10～20 parts of selfheals, 5～10 parts of the bighead atractylodes rhizomes, 10～20 parts of Buddha's hands, 5～10 parts, rascal,5～10 parts of cassia seeds, 30～50 parts of ginkgo leaves, 10～20 parts of pyrolas, 5～11 parts of cassia twig,10～30 parts of scythian lamb rhizomes, 6～16 parts of yncaria stems with hooks.
In raw material of the present invention:
Polygonum multiflower knotweed is rattan or the leaf bine stem of polygonum multiflorum thunb. Nature and flavor: sweet micro-hardship, flat.Function cures mainly: nourish heart, calm the nerves, dredging collateral, dispels the wind. Control insomnia, internal lesion caused by overexertion, hidrosis, deficiency of blood bodily pain,Ulcer, scrofula, wind sore mange.
Selfheal is the dry fruit ear of labiate selfheal PrunellavulgarisL.. PropertyTaste: pungent, bitter, cold. Function cures mainly: relieves inflammation or internal heat, and improving eyesight, dissipating bind, detumescence. For red eye, swell pain,Order pearl nyctalgia, has a headache dizzy, scrofula, and goiter and tumor, acute mastitis swells and ache; Thyroid gland enlargement, scrofula,The proliferation of mammary gland, hypertension.
The bighead atractylodes rhizome is the dry root of feverfew bighead atractylodes rhizome AtractylodesmacrocephalaKoidz.Stem. Nature and flavor: bitter, sweet, temperature. Function cures mainly: strengthening the spleen and replenishing qi, and stomach, eliminating dampness Li Shui.
Buddha's hand is Rutaceae citrus plant Buddha's hand CitrusmedicaL.var.sarcodactylisThe dry fruit of Swingle. Nature and flavor: pungent, bitter, sour, temperature. Function cures mainly: liver-smoothing, qi-regulating, andStomach and alleviating pain. For stagnation of liver-QI and stomach-QI, distending pain in the chest and hypochondrium, gastral cavilty ruffian is full, the few vomiting of food.
Rascal is doing of rutaceae orange CitrusreticulataBlanco and variety thereofThe pericarp of dry young fruit or immature fruit. Nature and flavor: bitter, pungent, temperature. Function cures mainly: soothing the liver relieving stagnant Qi,Long-pendingization that disappear is stagnant. For distending pain in the chest and hypochondrium, hernia, newborn core, acute mastitis, dyspeptic abdominalgia.
Cassia seed is legume Cassia tora CassiaobtusifoliaL. or little Cassia tora CassiatoraL. dry mature seed. Nature and flavor: sweet, bitter, salty, be slightly cold. Function cures mainly: removing heat to brighten vision, profitIntestines defaecation. For the puckery pain of hot eyes, the many tear of photophobia, has a headache dizzy, and order is secretly not clear, constipation.
Pyrola is the herb of Pyrolaceae plant wintergreen or Pylora rotundolia etc. Nature and flavor: sweetness and bitterness,Temperature. Function cures mainly: can qi-restoratives, kidney-nourishing, dispel rheumatism, promoting blood circulation for regulating menstruation, the strong bone of kidney tonifying, cough-relieving,Hemostasis; Cure mainly: main lumbago due to the kidney deficiency, arthralgia due to wind-dampness, soft, the new chronic cough of muscles and bones impotence are coughed, haematemesis, bleeding from five sense organs or subcutaneous tissue,Uterine bleeding, traumatism and bleeding.
Ginkgo leaf, for Ginkgoaceae plant Ginkgo biloba (maidenhair tree, Gong Sunshu) GinkgobilobaL.'sDry leaf. Nature and flavor: sweet, bitter, puckery, flat; The thoughts of returning home, lung channel. Function cures mainly: astringes the lung, relievings asthma,Promoting blood circulation and removing blood stasis, pain relieving. Cough and breathe heavily for the deficiency syndrome of the lung; Coronary heart disease, angina pectoris, high fat of blood, anti-freezing is solid, hasCertain probability improves memory. Though scythian lamb rhizome can stimulate circulation, prevention cardiovascular disease, notCan take other simultaneously and control cardiovascular drugs (for example aspirin), ginkgo biloba extract is concentrated granular shape,There is very strong removing free radical and antioxidation, glycosides displayed, amino acid and ammonia in scythian lamb rhizomeThe synthetic collagen Proteins of base acid is improved looks to human body, and check melanin growth, keeps skin gloss and bulletProperty plays no small effect.
Cassia twig is the spray of canella Chinese cassia tree. Nature and flavor: acrid, sweet, warm. Function cures mainly: sweatingExpelling pathogenic factors from muscles and skin, warming meridian, helps yang transforming qi, the flat gas that spins. For anemofrigid cold, coldness and pain in the epigastrium, blood is coldThrough closing, joint pain, phlegm and retained fluid, oedema, palpitaition, globefish runs quickly.
Scythian lamb rhizome is the rhizome of Dicksoniaceae plant Cibotium barometz. Nature and flavor: bitter, sweet, warm in nature. MeritCan cure mainly: filling liver kidney, strengthen the bone, wines used as antirheumatic; Cure mainly kidney deficiency and liver, the acid of waist knee is cold, and leg is soft weak, andArthralgia due to wind-dampness. Rhizoma cibotii belongs to strong muscles and bones medicine, and the disease that muscles and bones is not good for has the dual work for the treatment of and health careWith.
Yncaria stem with hooks, for madder wort yncaria stem with hooks Uncariarhynch0phylla (Miq.) Jacks.,Largeleaf gambirplant branchlet UncariamacrophyllaWall., uncaria hirsuta UncariahirsutaHavil.,Uncaria sinensis Uncariasinensis (Oliv.) Havil. or stockless fruit yncaria stem with hooks UncariaSessilifructusRoxb. dry buckle stem branch. Nature and flavor: sweet, cool. Function cures mainly: heat-clearingFlat liver, dispelling wind and relieving convulsion. Be used for having a headache dizzy, dysphoric cold, frightened epilepsy is twitched, pre-eclampsia; High bloodPress.
Preferably, pocket mushrooms culture medium specialist additive of the present invention, former by following weight portionMaterial is made: 30 parts of Polygonum multiflower knotweeds, and 15 parts of selfheals, 8 parts of the bighead atractylodes rhizomes, 15 parts of Buddha's hands, 7 parts, rascal,8 parts of cassia seeds, 40 parts of ginkgo leaves, 15 parts of pyrolas, 8 parts of cassia twig, 20 parts of scythian lamb rhizomes, hook11 parts, rattan.
Additive of the present invention addition in the culture medium application of pocket mushrooms be 2 of culture medium total amount～7％。
Beneficial effect of the present invention is:
1, the material combination of pocket mushrooms culture medium specialist additive of the present invention is scientific and reasonable, synergy,Reasonable compatibility medicine on prescription, each other to correct its bias, eliminates or reduction toxicity raw materialIn contain a large amount of crude protein, crude fat, cellulose, lignin, mineral matter, amino acid, canAs the nutritional labeling of pocket mushrooms, reduce the amount of other major ingredient of culture medium, reduce costs.
2, additive of the present invention also contains the biological active matters such as alkaloid, polysaccharide, terpene, saponinsMatter, it has antibacterial and effect sterilization.
3, additive of the present invention also contains unknown growth promoting substance and can promote that pocket mushrooms fully absorbsNutritional labeling, the utilization rate of raising medium nutrient content, promotes the growth of pocket mushrooms, improves output,Improve nutrition and the local flavor of pocket mushrooms, after testing, amino acid content increases simultaneously.
4, additive of the present invention Chinese medicine material used can absorb and specify in culture medium before inoculationHeavy metal, as lead, cadmium, arsenic, mercury etc., effectively solves because pocket mushrooms belongs to mushroom, has heavy metalAccumulation ability, can almost absorb the heavy metal ion being dissolved in water completely, easily causes pocketThe content of beary metal of mushroom exceeds standard, and human body is eaten, and threatens the problem of health.
5, additive of the present invention can strengthen the anticancer and anti-oxidation efficacy of pocket mushrooms.
Detailed description of the invention
Below in conjunction with embodiment, the invention will be further described, but the present invention is not limited to these in factExecute example.
Embodiment 1
Take raw material by following weight portion: 20 parts of Polygonum multiflower knotweeds, 10 parts of selfheals, 5 parts of the bighead atractylodes rhizomes, Buddhist20 parts, hand, 10 parts, rascal, 5 parts of cassia seeds, 50 parts of ginkgo leaves, 10 parts of pyrolas, cassia twig 11Part, 30 parts of scythian lamb rhizomes, 6 parts of yncaria stems with hooks; Mix, be crushed to 20 orders.
Embodiment 2
Take raw material by following weight portion: 25 parts of Polygonum multiflower knotweeds, 13 parts of selfheals, 7 parts of the bighead atractylodes rhizomes, Buddhist18 parts, hand, 8 parts, rascal, 7 parts of cassia seeds, 45 parts of ginkgo leaves, 13 parts of pyrolas, 9 parts of cassia twig,25 parts of scythian lamb rhizomes, 8 parts of yncaria stems with hooks; Mix, be crushed to 40 orders.
Embodiment 3
Take raw material by following weight portion: 30 parts of Polygonum multiflower knotweeds, 15 parts of selfheals, 8 parts of the bighead atractylodes rhizomes, Buddhist15 parts, hand, 7 parts, rascal, 8 parts of cassia seeds, 40 parts of ginkgo leaves, 15 parts of pyrolas, 8 parts of cassia twig,20 parts of scythian lamb rhizomes, 11 parts of yncaria stems with hooks; Mix, be crushed to 30 orders.
Embodiment 4
Take raw material by following weight portion: 35 parts of Polygonum multiflower knotweeds, 18 parts of selfheals, 9 parts of the bighead atractylodes rhizomes, Buddhist13 parts, hand, 6 parts, rascal, 9 parts of cassia seeds, 35 parts of ginkgo leaves, 18 parts of pyrolas, 7 parts of cassia twig,15 parts of scythian lamb rhizomes, 13 parts of yncaria stems with hooks; Mix, be crushed to 50 orders.
Embodiment 5
Take raw material by following weight portion: 40 parts of Polygonum multiflower knotweeds, 20 parts of selfheals, 10 parts of the bighead atractylodes rhizomes, Buddhist10 parts, hand, 5 parts, rascal, 10 parts of cassia seeds, 30 parts of ginkgo leaves, 20 parts of pyrolas, cassia twig 5Part, 10 parts of scythian lamb rhizomes, 16 parts of yncaria stems with hooks; Mix, be crushed to 60 orders.
Test example 1:
In conventional pocket mushrooms culture medium, add pocket mushrooms culture medium specialist additive of the present invention and notThe pocket mushrooms comparative result out of medium culture adding is in table 1:
Table 1 is containing the pocket mushrooms comparative result of additive and conventional medium cultivation
Test example 2: the antioxidation of additive of the present invention to the pocket mushrooms of cultivating
1, material: (add the embodiment of the present invention 2 to conventional pocket mushrooms culture medium, addition is A groupCulture medium total amount 5%), B group (adds the embodiment of the present invention 4 to conventional pocket mushrooms culture medium, addsAmount is culture medium total amount 5%) and the pocket mushrooms cultivated respectively of comparative example (conventional medium).
2, test method:
(1) pocket mushrooms runic thing is prepared extracting method: get that A group, B group and comparative example cultivate respectivelyPocket mushrooms 10g, pulverizes respectively, by solid-liquid ratio 1:25 extracting in water, extracts three times, merges extract,Be concentrated into certain volume, add the ethanol precipitation of 3 times 95%, get supernatant concentration, dry, obtain pocket mushroomsCrude extract is for subsequent use.
(2) mensuration of reducing power: sample is by the potassium ferricyanide (K3Fe(CN)6) be reduced into potassium ferrocyanide(K4Fe(CN)6), potassium ferrocyanide again with Fe3+Effect, generates ferric ferrocyanide (Prussian blue), withAt 700nm wavelength, the size that Prussian blue absorbance represents reducing power is detected at place, and absorbance is higher,The reducing power of sample is just stronger, and non-oxidizability is stronger. At 700nm wavelength place, measure respectively concentration and beThe light absorption value of the pocket mushrooms CE that A group, B group and the comparative example of 5mg/ml cultivated respectively.
(3) remove superoxide anion experiment: producing ultra-oxygen anion free radical (O2-) reaction bodyIn system, adding respectively concentration is that the pocket mushrooms cultivated respectively of A group, B group and the comparative example of 5mg/ml is thickExtract, measures their clearance rates.
(4) experiment of removing hydroxyl radical free radical: FeSO4With H2O2Reaction produces OH, in system, addsEnter salicylic acid, can catch hydroxyl radical free radical and produce coloring matter, this material has maximum at 510nm placeAbsorb, in the time there is OH free radical scavenger in reaction system, this oxidizing process is suppressed, and inhalesShading value reduces. At FeSO4With H2O2In reaction system, adding respectively concentration is the A of 30mg/mlThe pocket mushrooms CE that group, B group and comparative example are cultivated respectively, measures clearance rate.
(5) NO under SGF pH condition2-Cleaning reaction effect: cleaning nitrite is effectively anti-Stop one of carcinogenic approach of N-nitroso compound. In simulated system, add and add respectively concentration to beThe pocket mushrooms CE that A group, B group and the comparative example of 14mg/ml cultivated respectively, measures clearance rate.
(6) DPPH method mensuration pocket mushrooms antioxidation activity: DPPH is a kind of stable in organic solventFree radical, be widely used in anti-oxidant appraisement system. In system, adding respectively concentration is 30mg/mlThe pocket mushrooms CE cultivated respectively of A group, B group and comparative example, measure clearance rate.
3, result of the test:
The reducing power of the pocket mushrooms CE that table 2 different culture media is cultivated
| A group | B group | Comparative example | |
| Light absorption value | 1.424 | 1.442 | 0.834 |
Table 2 result shows, the pocket mushrooms CE that A group, B group and comparative example are cultivated has anti-oxidantProperty, A group and B group non-oxidizability are better than comparative example.
The pocket mushrooms CE that table 3 different culture media is cultivated is removed the ability of superoxide anion
| A group | B group | Comparative example | |
| Clearance rate | 49％ | 43％ | 19％ |
Table 3 shows, the pocket mushrooms CE that A group, B group and comparative example are cultivated all has the super oxygen of removingThe ability of anion, A group and B group removing ability are better than comparative example.
The pocket mushrooms CE that table 4 different culture media is cultivated is removed the ability of hydroxyl radical free radical
| A group | B group | Comparative example | |
| Clearance rate | 81％ | 79％ | 44％ |
Table 4 shows, the pocket mushrooms CE that A group, B group and comparative example are cultivated all has removing hydroxylThe ability of free radical, A group and B group removing ability are better than comparative example.
The pocket mushrooms CE that table 5 different culture media is cultivated is to NO2-Scavenging action
| A group | B group | Comparative example | |
| Clearance rate | 58％ | 61％ | 41％ |
Table 5 shows, the pocket mushrooms CE that A group, B group and comparative example are cultivated is to NO2-There is removingEffect, A group and B group removing ability are better than comparative example.
The pocket mushrooms CE that table 6 different culture media is cultivated is removed effect to DPPH
| A group | B group | Comparative example | |
| Clearance rate | 66％ | 67％ | 33％ |
Table 6 shows, the pocket mushrooms CE that A group, B group and comparative example are cultivated has clearly DPPHExcept effect, A group and B group are removed effect and are better than comparative example.
Comprehensive above-mentioned oxidation reaction system result of the test, it is pocket that known A group, B group and comparative example are cultivatedMushroom has non-oxidizability, and A group and B group are better than comparative example, and the sleeve that adds additive of the present invention is describedPrecious mushroom culture medium can strengthen the anti-oxidation efficacy of pocket mushrooms.
Test example 3: the pocket mushrooms antitumaous effect that the present invention cultivates
1, animal used as test: healthy Kunming mouse, body weight (22 ± 3) g, male and female half and half, are cured by GuangxiUniversity of section Experimental Animal Center provides.
2, tumor cell line: murine sarcoma S180Ascit form, by Guangxi, treatment and prevention of tumour research institute is carriedSupply.
3, pocket mushrooms medicament preparation:
(add the embodiment of the present invention 2 to conventional pocket mushrooms culture medium, addition is for cultivating to get respectively A groupBase total amount 5%), the pocket mushrooms 10g that turns out of comparative example (conventional medium), be placed in 1000mLGlass beaker in, the 500mL that adds water, soaks after 1h, is placed on fire and decocts, after first intense fire boilsChange slow fire into, then stop working after decocting 60min, by centrifugal liquid 3500r/min 5min, take out supernatantLiquid. And then add 300mL water in sediment, decoct again twice with same method. By on 3 timesClear liquid medicine mixing is placed on slow fire, and concentrated constant volume becomes 200mL, makes the original liquid containing crude drug 5%,By this liquid sealing, be placed in refrigerator cold-storage and save backup.
3, experimental technique:
Get 60 of (22 ± 3) g mouse, the subcutaneous inoculation respectively through kunming mouse of forelimb gone down to posterity the 7th dayMurine sarcoma S180The abdominal cavity dilution of knurl strain cell (expecting that through platform blue dyeing viable count is greater than 95%)0.2mL, its concentration 5 × 105/mL. Mouse after injection oncocyte is divided into 3 groups, every group at random10, male and female half and half: A group, homonymy coordination injection pocket mushrooms decoction dilution 0.25mL, whole dosage isEvery injected in mice pocket mushrooms composition 0.5mg, continuously note 10 times; Comparative example group, homonymy coordination notePenetrate pocket mushrooms decoction dilution 0.25mL, whole dosage is every injected in mice pocket mushrooms composition 0.5mg,Note 10 times continuously; Control group, homonymy coordination injecting normal saline 0.5mL. Observe the life shape of mouseThe existence situation of state, tumor growth situation and mouse.
4, result of the test:
From experiment, the weather of observing at any time every mouse. Control group mice starts for 1 weekOccur the visible lumps of naked eyes (about 0.05cm × 0.05cm), A group, comparative example group have no naked eyesVisible lump. A group, comparative example group mouse all survive. Continue to observe and within the 16th day, rise to testing,Control group mice starts dead gradually, all dead to 36 days control groups. And A group, comparative example group are littleMouse is all survivals, and all do not occur the visible lump of naked eyes. Continue to observe to the 96th day comparative example groupThere is the visible lump of naked eyes in mouse, continues to observe and within the 106th day, rise to testing, and mouse starts comparative example groupDead gradually, all dead to 126 days comparative example group mouse, A group mouse all survives, and does not occur meatThe visible lump of eye, continues to observe still all survivals to 160 days. This experiment repeats 4 times altogether, result baseThis is identical. The existence situation of mouse is in table 7.
The anti-mouse S of pocket mushrooms that table 7 different culture media is cultivated180The experiment observed result of ascitogenous sarcoma
Pocket mushrooms anticancer test result shows, the pocket mushrooms that A group, comparative example are cultivated is to S180Ascitic type meatOncocyte has inhibitory action, and A group is better than comparative example, and the pocket mushrooms that adds additive of the present invention is describedCulture medium can strengthen the antitumaous effect of pocket mushrooms.
Claims (2)
1. pocket mushrooms culture medium specialist additive, is characterized in that, is made up of the raw material of following weight portion:20～40 parts of Polygonum multiflower knotweeds, 10～20 parts of selfheals, 5～10 parts of the bighead atractylodes rhizomes, 10～20 parts of Buddha's hands, green grass or young crops5～10 parts of skins, 5～10 parts of cassia seeds, 30～50 parts of ginkgo leaves, 10～20 parts of pyrolas, osmanthus5～11 parts, branch, 10～30 parts of scythian lamb rhizomes, 6～16 parts of yncaria stems with hooks.
2. pocket mushrooms culture medium specialist additive according to claim 1, is characterized in that, byThe raw material of following weight portion is made: 30 parts of Polygonum multiflower knotweeds, 15 parts of selfheals, 8 parts of the bighead atractylodes rhizomes, Buddha's hand 15Part, 7 parts, rascal, 8 parts of cassia seeds, 40 parts of ginkgo leaves, 15 parts of pyrolas, 8 parts of cassia twig, gold20 parts of hair rhizoma cibotii, 11 parts of yncaria stems with hooks.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106520571A (en) * | 2016-11-14 | 2017-03-22 | 郑学成 | Culture medium for gastrodia elata honey fungus cultivating strains |
| CN107509529A (en) * | 2017-08-23 | 2017-12-26 | 佛山推启农业研究院（普通合伙） | A kind of artificial cultivation method for improving hickory chick amino acid content |
| CN108157061A (en) * | 2018-01-25 | 2018-06-15 | 桂林灌阳桂灌菌业科技开发有限公司 | A kind of weeds culture medium cultivated for hayashishita simulated wild glossy ganoderma and preparation method thereof |
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| CN105061100A (en) * | 2015-07-31 | 2015-11-18 | 桂林宏旺菌业股份有限公司 | Pleurotus geesteranus cultivation medium |
| CN105053063A (en) * | 2015-07-31 | 2015-11-18 | 桂林宏旺菌业股份有限公司 | Special pleurotus geesteranus culture medium additive |
| CN105110891A (en) * | 2015-07-31 | 2015-12-02 | 桂林宏旺菌业股份有限公司 | Pleurotus geesteranus culture medium |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105061100A (en) * | 2015-07-31 | 2015-11-18 | 桂林宏旺菌业股份有限公司 | Pleurotus geesteranus cultivation medium |
| CN105053063A (en) * | 2015-07-31 | 2015-11-18 | 桂林宏旺菌业股份有限公司 | Special pleurotus geesteranus culture medium additive |
| CN105110891A (en) * | 2015-07-31 | 2015-12-02 | 桂林宏旺菌业股份有限公司 | Pleurotus geesteranus culture medium |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520571A (en) * | 2016-11-14 | 2017-03-22 | 郑学成 | Culture medium for gastrodia elata honey fungus cultivating strains |
| CN107509529A (en) * | 2017-08-23 | 2017-12-26 | 佛山推启农业研究院（普通合伙） | A kind of artificial cultivation method for improving hickory chick amino acid content |
| CN108157061A (en) * | 2018-01-25 | 2018-06-15 | 桂林灌阳桂灌菌业科技开发有限公司 | A kind of weeds culture medium cultivated for hayashishita simulated wild glossy ganoderma and preparation method thereof |
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Application publication date: 20160608 |