CN105639639A - Quinoa prepared powder rich in nutrient and preparation method thereof - Google Patents

Abstract

The invention discloses quinoa prepared powder rich in nutrient. The technology is characterized in that quinoa raw materials are roasted and cooked for 9 min at 150 DEG C, then are smashed and pass through a 80-mesh sieve; a basic ingredient bag contains oat, black beans, red beans, black sesame, apples, calcium caseinate, red dates, inulin, walnuts, collagen, Chinese wolfberry fruit, peanuts and yeast extracts, the oat, the black beans, the red beans, the black sesame, the red dates, the Chinese wolfberry fruit, the walnuts and the peanuts are smashed after being dried in hot air and pass through a 80-mesh sieve; the applies are smashed after being frozen and dried in vacuum and pass through a 80-mesh sieve, the raw materials are mixed according to the adding amount from a small quantity to a large quantity. A nutrient enrichment bag contains L-calcium lactate, vitamin A, thiamine hydrochloride, riboflavin, vitamin B6 and vitamin B12, and the components are evenly mixed through an equal-weight incremental method. A sensory adjusting agent is composed of brown sugar, tremella polysaccharide and sucralose, and the components are evenly mixed through an equal-weight incremental method. The sensory adjusting agent and the nutrient enrichment bag are evenly mixed, the mixture is mixed with the basic ingredient bag, and finally, the quinoa powder is added and evenly mixed.

Description

A kind of nutritious Quinoa reconstitutes powder and preparation method thereof

Technical field: the invention belongs to field of food.

Background technology:

In the modern life, food variety is numerous and diverse, and it is unbalanced that irrational diet structure easily causes human nutrition, thus being in sub-health state, finding and exploitation nutrition diversification food has become study hotspot in recent years. Quinoa is nutritious, almost with base substance Perfect Matchings needed for human life activity, can be listed in one of the whole world ten big nutraceutical, thus had significantly high edibility.

Quinoa originates in mountain area, South America Andean, is considered as " mother of grain " by ancient Inca, is the full corn of class of a kind of high nutritive value. World food and agricultural organization of the United Nations (FAO) assert that Quinoa is unique a kind of solitary plant meeting necessary for human basic nutrition material, formal recommendation its be the wholefood of the optimum mankind. The adequate proteins content of Quinoa is up to 16��20%, suitable with milk powder and meat, dietary fiber content is up to 7.1%, and rich in several mineral materials such as the several amino acids and calcium, magnesium, phosphorus, potassium, ferrum, zinc, selenium, the manganese that include 9 kinds of essential amino acids of needed by human body. It is of high nutritive value, cholesterol level is 0, heat is low, and there is easily ripe, easy to digest and unique mouthfeel, balanced can supplement the nutrients, regulate immunity and endocrine, prevention disease etc., thus it is applicable to all groups, it is particularly suited for the specific group such as " three high " crowd and infant, anemia of pregnant woman, student.

The eating method of Quinoa is a lot, edible two classes after being broadly divided into individually edible and processing. Although crude Quinoa seed is of high nutritive value, but there is saponin one layer pained on its surface, need to eliminate astringent taste through repeatedly brewing, meanwhile can cause that the nutritional labeling in Quinoa seriously runs off; Common Quinoa processing method has: makes characteristic dish, cook congee, Baoshang, make mixed juice beverage etc., but the too much course of processing can be destroyed the nutrient substance of Quinoa, reduce its edibility. Therefore, can by the Quinoa grind into powder after ripening, direct and other food materials mix and match such as corn, beans, making and reconstitute powder and eat, thus decreasing the course of processing, retaining while its original nutrient substance, supplement other nutrient substance of needed by human body and instant edible.

A kind of Quinoa cereal beverage and preparation method thereof application number: 201401749145.1 dates of application: 2014-12-09 the invention belongs to food technology field, is specifically related to a kind of Quinoa cereal beverage and preparation method thereof. With Quinoa, Herba bromi japonici, oligofructose, stabilizer for raw material, through the process of raw material, baking and pulverizing, allotment and constant volume, high pressure homogenize, ultra high temperature short time sterilization, fill are prepared. The present invention is balanced in nutrition, rich in the functional component of more dietary fiber Yu anti-three-hypers, can alleviate the affluenza problem being on the rise, the present invention has longer stability and relatively long storage life simultaneously, and as a kind of supping, portable is easily with, namely open and namely drink, instant. Eat suitable in anemia of pregnant woman, child, diabetes patient, three-hypers body constitution crowd.

Radix Et Rhizoma Fagopyri Tatarici Quinoa instant solid beverage prepare application number: the preparation method that 201410186451.9 dates of application: 2014-05-06 the invention provides a kind of Radix Et Rhizoma Fagopyri Tatarici Quinoa instant solid beverage, the preparation of this Radix Et Rhizoma Fagopyri Tatarici Quinoa instant solid beverage mainly comprises de-bitter Radix Et Rhizoma Fagopyri Tatarici powder, Quinoa powder and suitable auxiliary additive, this health food has blood fat reducing, blood pressure lowering, blood sugar lowering, treatment type ��diabetes mellitus, the intelligence promoting fetus and normal development of skeleton, it is prevented that iron deficiency anemia in pregnancy, anti-cancer, anti-ageing health-care effect of waiting for a long time. In addition, the Radix Et Rhizoma Fagopyri Tatarici powder of de-hardship is combined, with Quinoa powder, the instant solid beverage form being fabricated to instant edible by the present invention according to different proportion, it is easy to the different crowd Radix Et Rhizoma Fagopyri Tatarici Quinoa instant solid beverage needing to select to be suitable for according to oneself.

Vacuum lyophilization fruit juice Tremella Quinoa thick soup and production method application number: 201410088430.3 dates of application: 2014-03-12 the invention provides a kind of for the vacuum lyophilization fruit juice Tremella Quinoa thick soup of instant after infusing, and includes Tremella 60-85, fresh fruit concentrated juice 3-10, Quinoa powder surplus by weight percentage. Present invention also offers the production method of vacuum lyophilization fruit juice Tremella Quinoa thick soup. The method be by pretreated Tremella and concentrated fruit juice and de-bitter, softening, fry fragrant, pulverizing and be sieving through after ultra-fine Quinoa powder mix after, through vacuum lyophilization program, wet then through temperature control, control, resistance oxygen packaging, it is thus achieved that the fruit juice Tremella Quinoa thick soup of infusion instant. Fruit juice Tremella Quinoa thick soup volume is little, lightweight, easy to store and carry, rehydration is good, with above-mentioned fruit juice, Tremella, the local flavor of Quinoa, fragrance and smooth mouthfeel, rich in fruit juice, Tremella, the nutrition that human body is useful is closed different sexes by Quinoa, the crowd of all ages and classes eats, and has health-care effect.

Refer to puerperal fetus, Placenta Hominis give birth to after puerpera's health, genitals and psychological aspects adjust a period of time of recovery, need 6��8 weeks. Puerperal, crowd to recover own health on the one hand, promotes that systemic organs each system especially genitals recovers as early as possible, wants lactogenic and nurture baby on the one hand, therefore high than health adult for the demand of nutrient.

From threpsology's angle analysis Quinoa rich in multiple nutrients material, but how to allow human body is more effective, absorb its nutrient substance more fully, reach balancing nutrients in body composition, alleviate organism fatigue, improve the purpose of immunity, and be suitable to the edible of the Diseases such as hyperglycemia, hypertension, hyperlipidemia and have become as current problem demanding prompt solution, thus the exploitation of Quinoa edibility still had wide market prospect.

The present invention provides a kind of nutritious Quinoa to reconstitute powder and preparation method thereof,

A kind of Quinoa reconstitutes the process characteristic of powder and says down:

The pretreatment of Quinoa: adopting baking technology, Quinoa raw material is carried out ripening, cooking conditions is 150 DEG C, 9min.Then with micromill, the Quinoa of ripening is pulverized, cross 80 mesh sieves, obtain Quinoa powder.

Basis ingredient bags: include Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Mali pumilae, calcium caseinate, Fructus Jujubae, inulin, Semen Juglandis, collagen protein, Fructus Lycii, Semen arachidis hypogaeae, yeast extract, wherein first Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Jujubae, Fructus Lycii, Semen Juglandis and Semen arachidis hypogaeae carry out hot air drying, then pulverize, cross 80 mesh sieves, obtain corresponding ripe powder. Fructus Mali pumilae is pulverized after adopting vacuum lyophilization, crosses 80 mesh sieves. Above-mentioned raw materials is mixed from less to more according to addition.

Fortification bag: include L-calcium lactate, vitamin A, thiamine hydrochloride, riboflavin, vitamin B6 and vitamin B12. By above-mentioned nutrition enhancer according to equal increments method mix homogeneously.

Sense organ regulator: include brown sugar, tremella polysaccharide and sucralose, by these compositions according to equal increments method mix homogeneously.

By sense organ regulator and the mixing of fortification bag, then mix with basis ingredient bags, be eventually adding the mixing of Quinoa powder.

Edible explanation

This product one day edible 100g of suggestion. Using the little spoon given, often flat spoon about 10g when reconstituting, take 5 flat spoonfuls of formula powders, 50g, puts into a glass water, measures 250mL water altogether, and water temperature, more than 85 DEG C, is poured in formula powder, stirred, slightly cool a moment and edible.

The formula that the following three kinds of Quinoa prepared according to this technique reconstitute powder is composed as follows:

Table 1 Quinoa reconstitutes powder formula 1

Table 2 Quinoa reconstitutes powder formula 1 nutrient content

ARE is retinol equivalent. 1gRE=3.33IU vitamin A=1g alltrans retinol (vitamin A). Vitamin A only includes preformed retinol, does not include any carotenoid component when calculating and claiming vitamin A-active.

B calciferol, 1g vitamin D=40IU vitamin D.

C1mg ��-TE (alpha-tocopherol equivalent)=1mgd-alpha-tocopherol.

D nicotinic acid does not include precursor forms.

Further providing for another kind of nutritious Quinoa and reconstitute powder and preparation method thereof, constituent part is composed as follows:

Basis ingredient bags: including Herba bromi japonici, Semen Phaseoli, Semen sojae atricolor, Fructus Mali pumilae, Semen Sesami Nigrum, defatted milk powder and calcium caseinate, wherein first Herba bromi japonici, Semen Phaseoli, Semen sojae atricolor and Semen Sesami Nigrum carry out hot air drying, then pulverizes, crosses 80 mesh sieves, obtain corresponding ripe powder. Pulverize after Fructus Mali pumilae vacuum lyophilization, cross 80 mesh sieves. Above-mentioned raw materials is mixed from less to more according to addition.

Fortification bag: include L-calcium lactate, vitamin C, vitamin A palmitate powder, thiamine hydrochloride, riboflavin, vitamin B6, vitamin B12 and nicotiamide. By above-mentioned nutrition enhancer according to equal increments method mix homogeneously.

Sense organ regulator: include erythritol, oligofructose, tremella polysaccharide and sucralose, by these compositions according to equal increments method mix homogeneously.

Table 3 Quinoa reconstitutes powder formula 2

Table 4 Quinoa reconstitutes powder formula 2 nutrient content

ARE is retinol equivalent. 1gRE=3.33IU vitamin A=1g alltrans retinol (vitamin A). Vitamin A only includes preformed retinol, does not include any carotenoid component when calculating and claiming vitamin A-active.

B calciferol, 1g vitamin D=40IU vitamin D.

C1mg ��-TE (alpha-tocopherol equivalent)=1mgd-alpha-tocopherol.

D nicotinic acid does not include precursor forms.

Further providing for another kind of nutritious Quinoa and reconstitute powder and preparation method thereof, constituent part is composed as follows:

Basis ingredient bags: include Herba bromi japonici, soybean protein isolate, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Mali pumilae, purple rice and yeast powder, wherein first Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum and purple rice carry out hot air drying, then pulverize, cross 80 mesh sieves, obtain corresponding ripe powder.Pulverize after Fructus Mali pumilae vacuum lyophilization, cross 80 mesh sieves. Above-mentioned raw materials is mixed from less to more according to addition.

Fortification bag: include L-calcium lactate, vitamin A palmitate powder, vitamin B6 and vitamin B12. By above-mentioned nutrition enhancer according to equal increments method mix homogeneously.

Table 5 Quinoa reconstitutes powder 3

Table 6 Quinoa reconstitutes powder 3 nutrient content

ARE is retinol equivalent. 1gRE=3.33IU vitamin A=1g alltrans retinol (vitamin A). Vitamin A only includes preformed retinol, does not include any carotenoid component when calculating and claiming vitamin A-active.

B calciferol, 1g vitamin D=40IU vitamin D.

C1mg ��-TE (alpha-tocopherol equivalent)=1mgd-alpha-tocopherol.

D nicotinic acid does not include precursor forms.

Further developed the novel Quinoa adding ferment powder, to reconstitute the parts by weight of powder composed as follows:

Quinoa powder 45-55, basis ingredient bags 38-45, fortification bag 1.8-2, sense organ regulator 0.3-2, ferment powder 3-10, Lactobacillus plantarum powder 1-3.

Quinoa powder, preparation method thereof is as follows: Quinoa adopts baking technology, and Quinoa raw material is carried out ripening, and cooking conditions is stoving temperature 150 DEG C, 9min. Then with micromill, the Quinoa of ripening is pulverized, cross 80 mesh sieves, obtain Quinoa powder.

The parts by weight of basis ingredient bags are composed as follows

Herba bromi japonici 20-25, Semen sojae atricolor 13-16, Semen Phaseoli 8-13, Semen Sesami Nigrum 5-8, Fructus Mali pumilae 0.5-1, calcium caseinate 3-5, Fructus Jujubae 2-5, inulin 3-6, Semen Juglandis 1-4, collagen protein 3-5, Fructus Lycii 0.2-1, Semen arachidis hypogaeae 1-2, yeast extract 0.1-0.3;

Basis ingredient bags preparation method is as follows

Wherein first Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Jujubae, Fructus Lycii, Semen Juglandis and Semen arachidis hypogaeae carry out hot air drying, then pulverize, cross 80 mesh sieves, obtain corresponding ripe powder. Fructus Mali pumilae is pulverized after adopting vacuum lyophilization, crosses 80 mesh sieves. The raw material of basis ingredient bags is mixed from less to more according to addition.

Fortification bag parts by weight are composed as follows: include L-calcium lactate 20-25, retinol1-3, thiamine hydrochloride 1-2, riboflavin 1, vitamin B6 1, vitamin B12 0.5-1.

Fortification packet preparation method is as follows: by above-mentioned nutrition enhancer mix homogeneously.

Sense organ regulator parts by weight are composed as follows: brown sugar 5-8, tremella polysaccharide 0.2-1, sucralose 0.1-0.4;

The preparation method of sense organ regulator is as follows: by these composition mix homogeneously.

By sense organ regulator and the mixing of fortification bag, then mix with basis ingredient bags, be eventually adding the mixing of Quinoa powder.

Ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, is filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtains fermented liquid, and fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder. Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours; Then lyophilizing is until water content is less than 5%.

Pickle production method is with reference to Chinese patent 201110421967.3 or 201210310308.7 preparation.

The preparation method of Lactobacillus plantarum mycopowder is as follows:

Lactobacillus plantarum powdery mycopowder, production stage is as follows: slant strains is transferred to fluid medium the volume required that spreads cultivation step by step; The bacterium solution obtained spreading cultivation is centrifuged separating, and collects precipitation thalline; In precipitation thalline, add protective agent and be diluted; Utilizing drying equipment to prepare powdery microbial inoculum, it is (0.1��9.0) * (10 that said method prepares viable count in Lactobacillus plantarum mycopowder¹⁰) individual/gram, starch can be added during mycopowder mixing and dextrin realizes the proper ratio of each bacterium viable count.Described Lactobacillus plantarum is CGMCC11763.

Quinoa nutrient formulation powder, preparation method thereof is as follows: by this formula for a product proportionally mix homogeneously, pack.

Containing lactic acid in ferment powder, vegetable water solublity nutritional labeling vitamin, lactic acid bacteria. Above-mentioned ferment powder and Lactobacillus plantarum add in product, have substantially protection the intestines and stomach effect.

Specific embodiment

Described Lactobacillus plantarum is CGMCC11763.

Lactobacillus plantarum probiotic properties is as follows:

Lactobacillus plantarum CGMCCNO.11763 provided by the present invention is found to survive when pH is 1.50 through experiment, still in existing state after 1% cholate is cultivated 4 hours; Lactobacillus plantarum CGMCCNO.11763 degrading nitrite speed is fast, and capacity of decomposition reaches 10.9mg/h/kg, and this strain is when producing Pickles, and whole sweat nitrite concentration is at below 4.8mg/kg; Degrading rate of cholesterol, after fermentation 60h hour, can be reached 64.76% by CGMCCNO.11763. CGMCCNO.11763 Adhering capacity measure from coagulation rate be 95.71%.

CGMCCNO.11763 bacterial strain is to cholesterol degradation capability study and mensuration:

Take 1mlCGMCCNO.11763 mother solution and be inoculated in MRS cholesterol fluid medium (the cholesterol level 0.1mg/ml of 10mL, pH6.2) in, the constant temperature of 37 DEG C stands and cultivates 20h respectively, 40h, 60h is standby, with the MRS cholesterol culture medium that accesses 1mL sterilized water for comparison, take bacteria liquid sample and the comparison each 1ml of liquid of above cultivation different time, 9000r/min, centrifugal 10min at 4 DEG C, obtain fermented supernatant fluid, in o-phthalaldehyde method mensuration supernatant, cholesterol level is (particularly as follows: take each supernatant 0.1ml in corresponding test tube, add glacial acetic acid 0.3ml, the o-phthalaldehyde(OPA) 0.15ml of 1mg/ml, it is slowly added into concentrated sulphuric acid 1.0ml, mix homogeneously. room temperature stands 10min, surveys light absorption value under 550nm). each process 3 repetition, in kind makes cholesterol standard curve, calculates cholesterol level and degradation rate in supernatant, and result is in Table 1. it can be seen that cholesterol is had good Degradation by CGMCCNO.11763, after fermentation 60h hour, degradation rate can reach 64.76%.

The table 1 degraded situation to cholesterol.

The bile tolerance test of CGMCCNO.11763 bacterial strain:

Take CGMCCNO.11763 bacterium solution 1mL and inoculate strain in the 10mLMRS fluid medium (PH=6.4) containing different cholate (concentration gradients is 0.0%, 0.2%, 0.4%, 0.6%, 0.8%, 1%), be placed at 37 DEG C to cultivate respectively 0,2,4h, each process 3 repetition. Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilution factor solution, take 0.1ml diluent to be coated with in MRS, be inverted in 37 DEG C of biochemical cultivation cases and cultivate 48 hours (each dilution factor do 3 parallel) record and calculate the several number of bacterium on flat board. Result is in Table 2. This bacterium known increment of bacterium after gallbladder salinity is 1% process 4h still reaches 0.59 �� 0.92 �� 10⁷(cfu/ml), there is good bile tolerance ability.

Table 2 bile tolerance ability detection [(�� s) �� 10⁷cfu/ml]

The acid resistance test of CGMCCNO.11763 bacterial strain

Take CGMCCNO.11763 mother solution and inoculate strain in the 10mLMRS fluid medium of different pH value (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0) by 1ml, be placed at 37 DEG C to cultivate respectively 0,2,4h, each process 3 repetition. Respectively take 1ml sample bacterium solution to mix in 9ml normal saline, prepare dilute solution, take 0.1ml diluent and be coated with in MRS, in 37 DEG C of biochemical cultivation cases, be inverted the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board.Result is in Table 3. Illustrate that this bacterium has very strong acid-fast ability.

Table 3 acid-fast ability detection [(�� s) �� 10⁷cfu/ml]

The Adhering capacity of CGMCCNO.11763 bacterial strain measures

Cultivate CGMCCNO.11763 (MRS fluid medium), bacillus coli DH 5 alpha (LB fluid medium) 24h obtains fermentation liquid, be respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collect bacterium mud, wash bacterium mud 2 times with the sterile phosphate buffer (PBS) of pH=7.0 respectively and (in bacterium colony, namely add PBS, after concussion mix homogeneously, be placed in 3000r/min, centrifugal 10min at 4 DEG C, collect thalline). From coagulation rate (%): with aseptic PBS, bacterium mud CGMCCNO.11763 to be formed in the suspension bacteria liquid that light absorption value is 0.4 �� 0.1 (A0) and the bacteria suspension at wavelength 600nm place, measure light absorption value A24 after standing 24h, be (A0 A24)/A0 from coagulation rate (%) formula. ; His coagulation rate (%): the outstanding bacterium solution of CGMCCNO.11763 and bacillus coli DH 5 alpha is adjusted to the mix suspending bacterium solution that the light absorption value at wavelength 600nm place is 0.6 �� 0.1 (A0). Measuring light absorption value A24 after standing 24H, his coagulation rate (%) formula is (A0 A24)/A0. Measurement result is in Table 4, it is known that CGMCCNO.11763 is 95.71% from coagulation rate, has very strong Adhering capacity.

Table 4 Adhering capacity table

Bacterial strain physiological property

Described Lactobacillus plantarum (Lactobacillusplantarum) XH is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on November 30th, 2015, preserving number is CGMCCNO.11763, preservation address is: North Star West Road 1, Chaoyang District, city of BeiJing, China institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101.

This bacterial strain feature is as follows: examine under a microscope, and this bacterial strain is rod-short, and Gram��s staining is positive, atrichia, does not produce spore; On solid medium, this bacterium bacterium colony is white, and smooth surface is fine and close, and form is circular, and edge is more neat.

Physicochemical characteristics is: catalase (-), gelatin liquefaction (-), indole experiment (+), mobility (-), fermentation gas (-), nitrate reductase (-), fermentation gas (-), product hydrogen sulfide gas (-), pH4.0MRS culture medium grows (+). It is accredited as Lactobacillus plantarum (Lactobacillusplantarum), called after Lactobacillus plantarum (Lactobacillusplantarum) XH through Physiology and biochemistry.

This bacterial strain can at 57 DEG C well-grown, glucose tolerance is 275g/L.

Lactobacillus plantarum of the present invention, by gathering people Li Jianshu, separates in Yoghourt from Xinjiang Uygur fellow-villager family and obtains, acquisition time on June 2nd, 2015.

5L fermentation tank is tested

(1) Lactobacillus plantarum CGMCCNO.11763 mono-ring on inclined-plane is taken, access in the 250mL triangular flask equipped with 50mL culture medium MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm, cultivate about 12h, make thalline be in mid log phase for 37 DEG C.

(2) strain of logarithmic (log) phase is accessed in the 5L fermentation tank equipped with 3LMRS fluid medium (initial glucose is 150g/L). Inoculum concentration is 10%, and at 37 DEG C, 100rpm cultivates 8 hours, and logarithm early stage dissolved oxygen controls 10% (ventilation 0.5L/min), later stage Anaerobic culturel 63 hours. After fermentation ends, the lactic acid production of Lactobacillus plantarum CGMCCNO.11763 reaches 110g/L. Such lactic acid producing speed is beneficial to the rapid fermentation of Pickles.

(4) strain of logarithmic (log) phase is accessed in the 5L fermentation tank equipped with the 3L sodium nitrite liquid screening medium modified MRS screening culture medium of 2g/L sodium nitrite (the single nitrogenous source be).Inoculum concentration is 10%, and at 37 DEG C, 100rpm cultivates 8 hours, and logarithm early stage dissolved oxygen controls 10% (ventilation 0.5L/min), and later stage anaerobism, sweat adds the sodium nitrite solution of 20g/L according to the wear rate stream of nitrite, cultivates 2-3 days. After fermentation ends, calculate the sweat Lactobacillus plantarum CGMCCNO.11763 degradation rate to sodium nitrite. Found that: under this condition, the degradation rate of sodium nitrite can be reached 653mg/h/L by XH.

(5) accessing the strain 10mL of logarithmic (log) phase equipped with in Chinese cabbage pretreated for 2kg, traditionally Pickles method is processed, the content of nitrite measured in Pickles for every 12 hours. It was found that in whole sweat, the decomposition rate of sodium nitrite is 10.9mg/h/kg Chinese cabbage by XH bacterium. Content of sodium nitrite in Pickles is consistently lower than 4.8mg/kg, far below the content (20mg/kg) of regulation in standard GB/T 2714-2003.

Specific embodiment

Embodiment 1

The parts by weight that Quinoa reconstitutes powder are composed as follows:

Quinoa powder 50, basis ingredient bags 40, fortification bag 1.9, sense organ regulator 1, ferment powder 5, Lactobacillus plantarum powder 2.

Quinoa powder, preparation method thereof is as follows: Quinoa adopts baking technology, and Quinoa raw material is carried out ripening, and cooking conditions is 150 DEG C, 9min. Then with micromill, the Quinoa of ripening is pulverized, cross 80 mesh sieves, obtain Quinoa powder.

The parts by weight of basis ingredient bags are composed as follows

Herba bromi japonici 22, Semen sojae atricolor 15, Semen Phaseoli 10, Semen Sesami Nigrum 6, Fructus Mali pumilae 0.8, calcium caseinate 4, Fructus Jujubae 4, inulin 4, Semen Juglandis 2, collagen protein 4, Fructus Lycii 0.5, Semen arachidis hypogaeae 1, yeast extract 0.2;

Basis ingredient bags preparation method is as follows

Wherein first Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Jujubae, Fructus Lycii, Semen Juglandis and Semen arachidis hypogaeae carry out hot air drying, then pulverize, cross 80 mesh sieves, obtain corresponding ripe powder. Fructus Mali pumilae is pulverized after adopting vacuum lyophilization, crosses 80 mesh sieves. The raw material of basis ingredient bags is mixed from less to more according to addition.

Fortification bag parts by weight are composed as follows: include L-calcium lactate 22, dehydroretinol, thiamine hydrochloride 1, riboflavin 1, vitamin B6 1, vitamin B12 0.6.

Fortification packet preparation method is as follows: by above-mentioned nutrition enhancer according to equal increments method mix homogeneously.

Sense organ regulator parts by weight are composed as follows: brown sugar 7, tremella polysaccharide 0.5, sucralose 0.3;

The preparation method of sense organ regulator is as follows: by these compositions proportionally mix homogeneously.

Ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, is filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtains fermented liquid, and fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder. Cryodesiccated condition is :-30 degree pre-freezes 6 hours; Then lyophilizing is until water content is less than 5%.

Pickle production method is with reference to Chinese patent 201110421967.3 or 201210310308.7 preparation.

The preparation method of Lactobacillus plantarum mycopowder is as follows:

Lactobacillus plantarum powdery mycopowder, production stage is as follows: slant strains is transferred to fluid medium the volume required that spreads cultivation step by step; The bacterium solution obtained spreading cultivation is centrifuged separating, and collects precipitation thalline; In precipitation thalline, add protective agent and be diluted; Utilizing drying equipment to prepare powdery microbial inoculum, it is (0.1��9.0) * (10 that said method prepares viable count in Lactobacillus plantarum mycopowder¹⁰) individual/gram, starch can be added during mycopowder mixing and dextrin realizes the proper ratio of each bacterium viable count.Described Lactobacillus plantarum is CGMCC11763.

Quinoa nutrient formulation powder, preparation method thereof is as follows: by this formula for a product proportionally mix homogeneously, pack.

Embodiment 2 is basic with example 1

Quinoa powder 45, basis ingredient bags 45, fortification bag 1.8, sense organ regulator 2, ferment powder 10, Lactobacillus plantarum powder 1;

Quinoa powder, preparation method thereof is as follows: Quinoa adopts baking technology, and Quinoa raw material is carried out ripening, and cooking conditions is 150 DEG C, 9min. Then with micromill, the Quinoa of ripening is pulverized, cross 80 mesh sieves, obtain Quinoa powder;

The parts by weight of basis ingredient bags are composed as follows: Herba bromi japonici 22, Semen sojae atricolor 16, Semen Phaseoli 8, Semen Sesami Nigrum 8, Fructus Mali pumilae 0.5, calcium caseinate 5, Fructus Jujubae 2, inulin 3, Semen Juglandis 4, collagen protein 3, Fructus Lycii 0.2, Semen arachidis hypogaeae 1, yeast extract 0.3;

Basis ingredient bags preparation method is as follows:

Wherein first Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Jujubae, Fructus Lycii, Semen Juglandis and Semen arachidis hypogaeae carry out hot air drying, then pulverize, cross 80 mesh sieves, obtain corresponding ripe powder; Fructus Mali pumilae is pulverized after adopting vacuum lyophilization, crosses 80 mesh sieves. The raw material of basis ingredient bags is mixed from less to more according to addition;

Fortification bag parts by weight are composed as follows: include L-calcium lactate 20,3-Hydroxyretinol, thiamine hydrochloride 1, riboflavin 1, vitamin B6 1, vitamin B12 0.5;

Fortification packet preparation method is as follows: by above-mentioned nutrition enhancer mix homogeneously;

Sense organ regulator parts by weight are composed as follows: brown sugar 8, tremella polysaccharide 0.2, sucralose 0.4;

The preparation method of sense organ regulator is as follows: by these composition mix homogeneously;

By sense organ regulator and the mixing of fortification bag, then mix with basis ingredient bags, be eventually adding the mixing of Quinoa powder; Ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, is filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtains fermented liquid, and fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder; Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours; Then lyophilizing is until water content is less than 5%; Pickle production method is with reference to Chinese patent 201110421967.3 or 201210310308.7 preparation; Described Lactobacillus plantarum is CGMCC11763.

Quinoa nutrient formulation powder, preparation method thereof is as follows: by this formula for a product proportionally mix homogeneously, pack.

Embodiment 3

The present invention provides a kind of nutritious Quinoa to reconstitute powder and preparation method thereof,

The parts by weight that Quinoa reconstitutes powder are composed as follows:

Quinoa powder 55, basis ingredient bags 38, fortification bag 2, sense organ regulator 0.3, ferment powder 10, Lactobacillus plantarum powder 1.

Quinoa powder, preparation method thereof is as follows: Quinoa adopts baking technology, and Quinoa raw material is carried out ripening, and cooking conditions is 150 DEG C, 9min. Then with micromill, the Quinoa of ripening is pulverized, cross 80 mesh sieves, obtain Quinoa powder.

The parts by weight of basis ingredient bags are composed as follows

Herba bromi japonici 20, Semen sojae atricolor 16, Semen Phaseoli 13, Semen Sesami Nigrum 5, Fructus Mali pumilae 0.5, calcium caseinate 5, Fructus Jujubae 2, inulin 3, Semen Juglandis 1, collagen protein 5, Fructus Lycii 0.2, Semen arachidis hypogaeae 1, yeast extract 0.1;

Basis ingredient bags preparation method is as follows

Wherein first Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Jujubae, Fructus Lycii, Semen Juglandis and Semen arachidis hypogaeae carry out hot air drying, then pulverize, cross 80 mesh sieves, obtain corresponding ripe powder. Fructus Mali pumilae is pulverized after adopting vacuum lyophilization, crosses 80 mesh sieves.The raw material of basis ingredient bags is mixed from less to more according to addition.

Fortification bag parts by weight are composed as follows: include L-calcium lactate 20, retinol1, thiamine hydrochloride 2, riboflavin 1, vitamin B6 1, vitamin B12 0.5.

Fortification packet preparation method is as follows: by above-mentioned nutrition enhancer according to equal increments method mix homogeneously.

Sense organ regulator parts by weight are composed as follows: brown sugar 5, tremella polysaccharide 0.2, sucralose 0.1;

The preparation method of sense organ regulator is as follows: by these compositions according to equal increments method mix homogeneously.

By sense organ regulator and the mixing of fortification bag, then mix with basis ingredient bags, be eventually adding the mixing of Quinoa powder.

Ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, is filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtains fermented liquid, and fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder. Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours; Then lyophilizing is until water content is less than 5%.

Pickle production method is with reference to Chinese patent 201110421967.3 or 201210310308.7 preparation.

The preparation method of Lactobacillus plantarum mycopowder is as follows:

Lactobacillus plantarum powdery mycopowder, production stage is as follows: slant strains is transferred to fluid medium the volume required that spreads cultivation step by step; The bacterium solution obtained spreading cultivation is centrifuged separating, and collects precipitation thalline; In precipitation thalline, add protective agent and be diluted; Utilizing drying equipment to prepare powdery microbial inoculum, it is (0.1��9.0) * (10 that said method prepares viable count in Lactobacillus plantarum mycopowder¹⁰) individual/gram, starch can be added during mycopowder mixing and dextrin realizes the proper ratio of each bacterium viable count. Described Lactobacillus plantarum is CGMCC11763.

Quinoa nutrient formulation powder, preparation method thereof is as follows: by this formula for a product proportionally mix homogeneously, pack.

Table 7

Remarks: Quinoa powder (A), basis ingredient bags (B), fortification bag (C), ferment powder (D), Lactobacillus plantarum powder (E), face and spirit (X), effect of stimulating the secretion of milk (Y), the effect (W) of weight reducing

20.21.22 adopt example 1, example 2, example 3 component ratio

Edible explanation and effect experimental explanation

This product one day edible 100g of suggestion. Using little spoon when reconstituting, often flat spoon about 10g, take 5 flat spoonfuls of formula powders, altogether 50g, put into a glass water, measure 250mL water, water temperature, more than 85 DEG C, is poured in formula powder, is stirred, slightly cool a moment and edible.

Adopting 88 puerperas to do Product Validation experiment, every 4 groups carry out edible experiment, and result is table 7 such as.

Beneficial effect:

Puerperal formula nutrient content Main Basis DRIs in the various nutrient recommendations intakes of wet nurse. Puerperal, the nutrient adjustment of formula was on the attainable formula proportion basis of processing technique, required to carry out formula proportion adjustment according to the nutrient of puerpera's nutritional need feature and full nutritional formulas, to meet the nutritional need of puerpera. After carrying out formula adjustment with this, still can not meet full nutrient formulation requirement, reach requirement by adding the form of nutrition enhancer.

Puerpera, except the energy demand of self, also to supply the energy that the energy contained by milk and lactation process itself need, thus its to energy need increase. Regulation in the DRIs of revision in 2013, puerperal, the energy requirement of wet nurse increased 500kcal than health adult.

Protein is the important substance basis of vital movement, is also the base substance of repair tissue organ. Puerperal protein requirement comprises the following aspects, is first self nitrogen metabolism, and second is postpartum tissue reparation, including genitals's recovery, function rehabilitation, wound healing, stem cell the activity such as reparation and regeneration; 3rd is lactogenic, and the generation of milk needs to consume a large amount of dietary proteins, and lactogenic process can make internal nitrogen metabolism accelerate. Chinese Soclety of Nutrition advises, puerperal, wet nurse should increase 25g protein every day, reaches 80g every day.

In breast milk, fat content is higher, lipid is closely related with the brain development of baby, especially the long-chain polyunsaturated fatty acid therein growth particular importance to nervus centralis, in addition fat soluble vitamin absorption is also required to lipid, Chinese Soclety of Nutrition advises, fat intake accounts for the 20%��30% of gross energy. The fat mass that general wet nurse takes in is advisable with account for gross energy 27%. The energy supply ratio of its Linoleic acid and alpha-linolenic acid should reach 2.5%��9.0% and 0.5%��2%, and the suitable intake of DHA and EPA is 0.25g/d, and wherein the intake of DHA should reach 0.2g/d.

The requirement of wet nurse's calcium refer to maintain parent balance amount and galactopoiesis needed for calcium amount sum. If the intake of mother's dietary calcium can not meet needs, typically not affecting calcium content in galactopoiesis amount and milk, parent is by employing calcium in its skeleton to maintain stablizing of calcium level in milk. Its result is that wet nurse suffers from osteomalacia because of calcium deficiency, if things go on like this there will be the symptom such as waist and leg ache, tic. The recommended intake of wet nurse's calcium is 1000mg/d.

Ferrum element is the critical nutrients of fetal brain development, and the shortage of ferrum can cause baby's cognitive competence impaired risks to increase. Research shows, what store in fetus body is about available for after its birth nearly 6 months ferrum element used entirely from parent, and therefore ferrum element becomes one of nutrient that pregnancy and puerperium puerpera is easiest to lack. Additionally, blood loss is relatively big when puerpera produces, if supplementing the iron deficiency taken in, very easily there is the diseases such as iron deficiency anemia in puerpera. The recommended intake of wet nurse in puerperal ferrum every day is 24mg.

Most vitamin can be transported in milk by mammary gland, provides corresponding vitamin for baby. Therefore the requirement of vitamin is also increased by puerperal wet nurse accordingly.

Owing to vitamin D makes consumption very low as nutrition enhancer, even if carrying out fortification can not meet requirement. Therefore this formula does not use nutrition enhancer, final selection is rich in the yeast extract of vitamin D, the shortcoming of vitamin d insufficiency in supplement formula. In addition, by interpolation nutrition enhancer, vitamin A, vitamin B group and calcium are carried out fortification. It addition, puerpera is also very high for the requirement of ferrum, therefore this formula should increase the content of ferrum. By adding beyond requirement to iron content in fully nutrient formula food after nutrition enhancer, therefore finally select ferrum-rich saccharomyces cerevisiae extract, to reach to supplement the purpose of ferrum.

Formula high quality protein in puerperal after fortification accounts for the 69.1% of gross protein, and wherein animal protein accounts for the 35% of gross protein. The energy supply of three nutritious elements is than respectively protein 23.5%, fat 21.1%, carbohydrate 54.7%. The energy supply of linoleic acid and two kinds of essential fatty acid of alpha-linolenic acid is 10.4% and 1.2% than respectively. With the recipe ratio before fortification relatively, the change of total amount nutritional labeling is little, but the content of the multivitamin after fortification and mineral increases, and vitamin A, vitamin B group and calcium etc. have been satisfied by the requirement of wholefood.

Claims (10)

1. Quinoa reconstitutes a powder, following methods prepare:

The pretreatment of Quinoa: adopting baking technology, Quinoa raw material is carried out ripening, cooking conditions is 150 DEG C, 9min; Then the Quinoa of ripening is pulverized, cross 80 mesh sieves, obtain Quinoa powder;

Basis ingredient bags: include Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Mali pumilae, calcium caseinate, Fructus Jujubae, inulin, Semen Juglandis, collagen protein, Fructus Lycii, Semen arachidis hypogaeae, yeast extract, wherein first Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Jujubae, Fructus Lycii, Semen Juglandis and Semen arachidis hypogaeae carry out hot air drying, then pulverize, cross 80 mesh sieves, obtain corresponding ripe powder. Fructus Mali pumilae is pulverized after adopting vacuum lyophilization, crosses 80 mesh sieves; Above-mentioned raw materials is mixed;

Fortification bag: include L-calcium lactate, vitamin A, thiamine hydrochloride, riboflavin, vitamin B6 and vitamin B12. By above-mentioned nutrition enhancer mix homogeneously;

Sense organ regulator: include brown sugar, tremella polysaccharide and sucralose, by these composition mix homogeneously;

By sense organ regulator and the mixing of fortification bag, then mix with basis ingredient bags, be eventually adding the mixing of Quinoa powder.

2. Quinoa reconstitutes powder according to claim 1, and described Quinoa reconstitutes shown in powder formula table composed as follows:

Table 1 Quinoa reconstitutes powder formula 1

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3. Quinoa reconstitutes powder according to claim 1, and described Quinoa reconstitutes shown in powder formula table composed as follows:

Table 3 Quinoa reconstitutes powder formula 2

4. Quinoa reconstitutes powder according to claim 1, and described Quinoa reconstitutes shown in powder formula table composed as follows.

Table 5 Quinoa reconstitutes powder 3

Material name Percentage ratio Quinoa 50 Herba bromi japonici 13.3 Semen Phaseoli 5.2 Semen sojae atricolor 7.5 3 --> Semen Sesami Nigrum 5 Fructus Mali pumilae 2.933 Soybean protein isolate 6 Purple rice 2 Yeast powder 2 L-calcium lactate 1.96623 Vitamin A palmitate powder 0.00092 Vitamin B6 0.00221 Vitamin B12 0.00064 Vitamin D yeast extract 0.02 Brown sugar 4 Tremella polysaccharide 0.07 Sucralose 0.007 Add up to 100

5. a kind of Quinoa reconstitutes powder according to claim 1, and the parts by weight that described Quinoa reconstitutes powder are composed as follows:

Quinoa powder 45-55, basis ingredient bags 38-45, fortification bag 1.8-2, sense organ regulator 0.3-2, ferment powder 3-10, Lactobacillus plantarum powder 1-3.

6. Quinoa reconstitutes powder according to claim 5, it is characterized in that, described ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, being filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtain fermented liquid, fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder; Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours; Then lyophilizing is until water content is less than 5%.

7. Quinoa reconstitutes powder according to claim 5, and described Lactobacillus plantarum is CGMCC11763.

8. Quinoa according to claim 5-7 reconstitutes powder, and the parts by weight that described Quinoa reconstitutes powder are composed as follows: Quinoa powder 50, basis ingredient bags 40, fortification bag 1.9, sense organ regulator 1, ferment powder 5, Lactobacillus plantarum powder 2.

9. Quinoa according to claim 5-7 reconstitutes powder, and the parts by weight that Quinoa reconstitutes powder are composed as follows: Quinoa powder 45, basis ingredient bags 45, fortification bag 1.8, sense organ regulator 2, ferment powder 10, Lactobacillus plantarum powder 1.

10. Quinoa according to claim 5-7 reconstitutes powder, and the parts by weight that Quinoa reconstitutes powder are composed as follows:

Quinoa powder 45, basis ingredient bags 45, fortification bag 1.8, sense organ regulator 2, ferment powder 10, Lactobacillus plantarum powder 1;

Quinoa powder, preparation method thereof is as follows: Quinoa adopts baking technology, and Quinoa raw material is carried out ripening, and cooking conditions is 150 DEG C, 9min.Then with micromill, the Quinoa of ripening is pulverized, cross 80 mesh sieves, obtain Quinoa powder;

The parts by weight of basis ingredient bags are composed as follows: Herba bromi japonici 22, Semen sojae atricolor 16, Semen Phaseoli 8, Semen Sesami Nigrum 8, Fructus Mali pumilae 0.5, calcium caseinate 5, Fructus Jujubae 2, inulin 3, Semen Juglandis 4, collagen protein 3, Fructus Lycii 0.2, Semen arachidis hypogaeae 1, yeast extract 0.3;

Basis ingredient bags preparation method is as follows:

Wherein first Herba bromi japonici, Semen sojae atricolor, Semen Phaseoli, Semen Sesami Nigrum, Fructus Jujubae, Fructus Lycii, Semen Juglandis and Semen arachidis hypogaeae carry out hot air drying, then pulverize, cross 80 mesh sieves, obtain corresponding ripe powder; Fructus Mali pumilae is pulverized after adopting vacuum lyophilization, crosses 80 mesh sieves. The raw material of basis ingredient bags is mixed from less to more according to addition;

Fortification bag parts by weight are composed as follows: include L-calcium lactate 20,3-Hydroxyretinol, thiamine hydrochloride 1, riboflavin 1, vitamin B6 1, vitamin B12 0.5;

Fortification packet preparation method is as follows: by above-mentioned nutrition enhancer mix homogeneously;

Sense organ regulator parts by weight are composed as follows: brown sugar 8, tremella polysaccharide 0.2, sucralose 0.4;

The preparation method of sense organ regulator is as follows: by these composition mix homogeneously;

By sense organ regulator and the mixing of fortification bag, then mix with basis ingredient bags, be eventually adding the mixing of Quinoa powder; Ferment powder preparation method is as follows: adopting the fermentation liquid that pickle fermentation produces discharge in latter stage is raw material, is filtered to remove the big grain materials such as the dish leaf in fermentation liquid and obtains fermented liquid, and fermented liquid adopts the drying meanss such as lyophilization to prepare ferment powder; Cryodesiccated condition is :-30--40 degree pre-freeze 6 hours; Then lyophilizing is until water content is less than 5%; Pickle production method is with reference to Chinese patent 201110421967.3 or 201210310308.7 preparation; Described Lactobacillus plantarum is CGMCC11763.