CN105621627B - One kind utilizes TiO2‑CeO2Load C YP119 enzyme photo catalytic reductions Cr6+Method - Google Patents
One kind utilizes TiO2‑CeO2Load C YP119 enzyme photo catalytic reductions Cr6+Method Download PDFInfo
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- 230000001699 photocatalysis Effects 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 title claims description 7
- 102000004190 Enzymes Human genes 0.000 title claims description 7
- JOPOVCBBYLSVDA-UHFFFAOYSA-N chromium(6+) Chemical compound [Cr+6] JOPOVCBBYLSVDA-UHFFFAOYSA-N 0.000 title description 3
- 238000010531 catalytic reduction reaction Methods 0.000 title 1
- 229910010413 TiO 2 Inorganic materials 0.000 claims abstract description 23
- 108700032333 Sulfolobus solfataricus CYP119 Proteins 0.000 claims abstract description 21
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 claims abstract description 7
- 229910002492 Ce(NO3)3·6H2O Inorganic materials 0.000 claims abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 24
- 239000002131 composite material Substances 0.000 claims description 8
- 238000001027 hydrothermal synthesis Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 8
- 239000007787 solid Substances 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 5
- 230000010757 Reduction Activity Effects 0.000 claims description 2
- 230000003197 catalytic effect Effects 0.000 claims 1
- 229910000422 cerium(IV) oxide Inorganic materials 0.000 claims 1
- 239000000463 material Substances 0.000 abstract description 6
- 239000003344 environmental pollutant Substances 0.000 abstract description 5
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 2
- 239000011941 photocatalyst Substances 0.000 abstract 1
- 239000011651 chromium Substances 0.000 description 14
- 239000000243 solution Substances 0.000 description 10
- 208000025865 Ulcer Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000007146 photocatalysis Methods 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 231100000397 ulcer Toxicity 0.000 description 2
- 206010000087 Abdominal pain upper Diseases 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 description 1
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 description 1
- 206010070840 Gastrointestinal tract irritation Diseases 0.000 description 1
- 206010067125 Liver injury Diseases 0.000 description 1
- 208000003420 Nasal Septal Perforation Diseases 0.000 description 1
- 206010028765 Nasal septum perforation Diseases 0.000 description 1
- 206010028813 Nausea Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 208000036071 Rhinorrhea Diseases 0.000 description 1
- 206010039101 Rhinorrhoea Diseases 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000011942 biocatalyst Substances 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000000711 cancerogenic effect Effects 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 208000001780 epistaxis Diseases 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 231100000234 hepatic damage Toxicity 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
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- 230000007774 longterm Effects 0.000 description 1
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- 210000002850 nasal mucosa Anatomy 0.000 description 1
- 210000000492 nasalseptum Anatomy 0.000 description 1
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- 230000002186 photoactivation Effects 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001878 scanning electron micrograph Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/342—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the enzymes used
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- B01J31/003—Catalysts comprising hydrides, coordination complexes or organic compounds containing enzymes
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- B01J31/00—Catalysts comprising hydrides, coordination complexes or organic compounds
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Abstract
本发明涉及一种利用TiO2‑CeO2负载CYP119酶光催化还原Cr6+的方法，所得材料能在光照下高效率地催化还原重金属环境污染物Cr6+。所述方法包括：将Ce(NO3)3·6H2O和钛酸正丁酯溶液混和均匀后，转至水热釜反应。反应结束后将样品分离，清洗，烘干后煅烧得到TiO2‑CeO2。以TiO2‑CeO2为载体负载CYP119酶制备得到光催化剂。其特征是利用TiO2‑CeO2负载CYP119酶，所得材料具有高效的光催化还原Cr6+的性能。
The invention relates to a method for photocatalytically reducing Cr 6+ by loading CYP119 enzyme on TiO 2 ‑CeO 2 . The obtained material can efficiently catalyze and reduce Cr 6+ , a heavy metal environmental pollutant, under light. The method comprises: mixing Ce(NO 3 ) 3 ·6H 2 O and n-butyl titanate solution evenly, and then transferring to a hydrothermal tank for reaction. After the reaction, the sample is separated, washed, dried and then calcined to obtain TiO 2 ‑CeO 2 . The photocatalyst was prepared by loading CYP119 enzyme on TiO 2 ‑CeO 2 . It is characterized in that TiO 2 -CeO 2 is used to load CYP119 enzyme, and the obtained material has the performance of highly efficient photocatalytic reduction of Cr 6+ .
Description
技术领域technical field
本发明涉及光催化及生物催化技术领域，尤其涉及一种利用TiO2-CeO2负载CYP119酶光催化还原Cr6+的方法。The invention relates to the technical field of photocatalysis and biocatalysis, in particular to a method for photocatalytically reducing Cr 6+ by using TiO 2 -CeO 2 to support CYP119 enzyme.
背景技术Background technique
CYP119酶是一种细胞色素P450酶，具有超常的稳定性和耐受性。它的热变性温度高达90℃，并可以耐受200 MPa以上的高压和极端的pH条件，是极佳的生物催化剂，是蛋白质工程的理想目标，在生物制药、生物合成等领域具有极其重要的应用价值。最近的研究显示其具有特别的光激活机制，这为构建基于CYP119酶的光催化体系提供了依据，使利用简单廉价的光催化体系代替复杂昂贵的辅助酶体系提供了可能，这将极大地推动酶催化体系在环境污染物治理中的实际应用。The CYP119 enzyme is a cytochrome P450 enzyme with exceptional stability and tolerance. Its thermal denaturation temperature is as high as 90°C, and it can withstand high pressure and extreme pH conditions above 200 MPa. It is an excellent biocatalyst and an ideal target for protein engineering. It has extremely important roles in the fields of biopharmaceuticals and biosynthesis. Value. Recent studies have shown that it has a special photoactivation mechanism, which provides a basis for the construction of a photocatalytic system based on CYP119 enzymes, making it possible to replace complex and expensive auxiliary enzyme systems with simple and cheap photocatalytic systems, which will greatly promote Practical application of enzyme-catalyzed systems in the treatment of environmental pollutants.
六价铬化合物在皮革、化工、电子产品中广泛应用，残留在环境中的Cr6+是一种严重的环境污染物。Cr6+在体内具有致癌作用，还会引起诸多的其他健康问题，如吸入某些较高浓度的六价铬化合物会引起流鼻涕、打喷嚏、瘙痒、鼻出血、溃疡和鼻中隔穿孔。短期大剂量的接触，在接触部位会产生不良后果，包括溃疡、鼻黏膜刺激和鼻中隔穿孔。摄入超大剂量的铬会导致肾脏和肝脏的损伤、恶心、胃肠道刺激、胃溃疡、痉挛甚至死亡。危害最大的是长期或短期接触或吸入时有致癌危险。因此，治理Cr6+污染意义重大。Hexavalent chromium compounds are widely used in leather, chemical, and electronic products, and Cr 6+ remaining in the environment is a serious environmental pollutant. Cr 6+ is carcinogenic in the body and can cause many other health problems, such as inhalation of certain higher concentrations of hexavalent chromium compounds can cause runny nose, sneezing, itching, epistaxis, ulcers and nasal septum perforation. Short-term high-dose exposure can produce adverse effects at the site of exposure, including ulcers, irritation of the nasal mucosa, and perforation of the nasal septum. Ingestion of very large doses of chromium can cause kidney and liver damage, nausea, gastrointestinal irritation, stomach ulcers, cramps and even death. The most harmful is the risk of cancer in long-term or short-term exposure or inhalation. Therefore, it is of great significance to control Cr 6+ pollution.
在本发明方法中，提供了一种构建基于CYP119酶光催化体系的方法。利用TiO2-CeO2复合材料作为载体，负载CYP119酶，构建复合光催化材料。所得产品对Cr6+有很好的光催化还原效果。In the method of the present invention, a method for constructing a photocatalytic system based on CYP119 enzyme is provided. The TiO 2 -CeO 2 composite material was used as a carrier to load CYP119 enzyme to construct a composite photocatalytic material. The obtained product has a good photocatalytic reduction effect on Cr 6+ .
本发明涉及一种利用TiO2-CeO2负载CYP119酶光催化还原Cr6+的方法，原料易得，成本低，操作简单，所述方法未见报道。The invention relates to a method for using TiO 2 -CeO 2 to load CYP119 enzyme to photocatalytically reduce Cr 6+ . The raw material is easy to obtain, the cost is low, and the operation is simple. The method has not been reported.
发明内容Contents of the invention
本发明的目的在于提供一种利用TiO2-CeO2负载CYP119酶光催化还原Cr6+的方法，本发明采取如下手段：The purpose of the present invention is to provide a method for photocatalytically reducing Cr 6+ by using TiO 2 -CeO 2 to support CYP119 enzyme, and the present invention adopts the following means:
一种利用TiO2-CeO2负载CYP119酶光催化还原Cr6+的方法，其特征在于：A method for using TiO 2 -CeO 2 to load CYP119 enzyme to photocatalytically reduce Cr 6+ , characterized in that:
（1）将Ce(NO3)3·6H2O溶解于乙醇，比例为0.1498 g Ce(NO3)3·6H2O/20 mL乙醇；(1) Dissolve Ce(NO 3 ) 3 ·6H 2 O in ethanol at a ratio of 0.1498 g Ce(NO 3 ) 3 ·6H 2 O/20 mL ethanol;
（2）将钛酸正丁酯溶解于乙醇和H2O的混和液，比例为352 ul钛酸正丁酯/20 mL乙醇和10 mL H2O的混和液；(2) Dissolve n-butyl titanate in the mixed solution of ethanol and H 2 O, the ratio is 352 ul n-butyl titanate/20 mL ethanol and 10 mL H 2 O mixed solution;
（3）将（1）和（2）所得溶液混和，超声分散均匀，转入水热反应釜120℃水热反应48h。将所得固体离心分离，干燥后450℃煅烧5 h，得到TiO2-CeO2复合材料；(3) Mix the solutions obtained in (1) and (2), disperse them uniformly by ultrasonic, transfer to a hydrothermal reaction kettle for hydrothermal reaction at 120°C for 48 hours. The resulting solid was centrifuged, dried and calcined at 450°C for 5 h to obtain a TiO 2 -CeO 2 composite material;
（4）将CYP119酶溶液与TiO2-CeO2复合材料通过持续搅拌混和分散均匀，然后离心分离，洗涤后冷冻干燥即得产品。(4) The CYP119 enzyme solution and the TiO 2 -CeO 2 composite material are continuously stirred and mixed to disperse evenly, then centrifuged, washed, and then freeze-dried to obtain the product.
所得的材料利用TiO2-CeO2复合材料负载CYP119酶，构建酶光催化体系。The obtained material utilizes the TiO 2 -CeO 2 composite material to load CYP119 enzyme to construct an enzyme photocatalytic system.
所得的材料具有良好的光催化还原活性，对Cr6+具有很好的光催化还原性能。The obtained material has good photocatalytic reduction activity and excellent photocatalytic reduction performance for Cr 6+ .
本发明的优点是：利用TiO2-CeO2作为载体负载CYP119酶，构建新型的酶光催化体系，使得CYP119酶的光活性能够得到很好的应用，对推动酶催化体系在环境污染物治理中的实际应用具有重要意义；原料易得，成本低，操作简单；所得产品具有优良的光催化还原性能，可利用光催化还原重金属环境污染物Cr6+。The advantages of the present invention are: using TiO 2 -CeO 2 as a carrier to load CYP119 enzymes to construct a new type of enzyme photocatalysis system, so that the photoactivity of CYP119 enzymes can be well applied, and to promote the enzyme catalysis system in the treatment of environmental pollutants The practical application is of great significance; the raw materials are easy to obtain, the cost is low, and the operation is simple; the obtained product has excellent photocatalytic reduction performance, and can use photocatalytic reduction of heavy metal environmental pollutants Cr 6+ .
附图说明Description of drawings
图1为本发明之实施例1所得到TiO2-CeO2的扫描电镜图；Fig. 1 is the scanning electron micrograph of TiO 2 -CeO 2 obtained in Example 1 of the present invention;
图2为本发明之实施例1所得到材料光催化还原Cr6+。Fig. 2 is the photocatalytic reduction of Cr 6+ by the material obtained in Example 1 of the present invention.
具体实施方式detailed description
实施例1Example 1
不断搅拌下将0.1498 g Ce(NO3)3·6H2O溶解于20 mL乙醇；在20 mL乙醇和10 mLH2O混和液中加入352 ul钛酸正丁酯；将上述两种溶液混和，通过超声分散均匀，再转入水热反应釜于120℃水热反应48 h后，将所得固体离心分离，干燥后于450℃煅烧5 h，得到TiO2-CeO2。将50 mg TiO2-CeO2分散于50 mL pH=6.5的磷酸钾缓冲溶液中，再加入200 uL的1 uM的CYP119酶溶液，持续搅拌20 min，离心分离得到固体，洗涤后冷冻干燥，即得到产品。Dissolve 0.1498 g Ce(NO 3 ) 3 6H 2 O in 20 mL ethanol under constant stirring; add 352 ul n-butyl titanate to the mixture of 20 mL ethanol and 10 mL H 2 O; mix the above two solutions, Uniformly dispersed by ultrasonic, then transferred to a hydrothermal reaction kettle for hydrothermal reaction at 120°C for 48 hours, the obtained solid was centrifuged, dried and calcined at 450°C for 5 hours to obtain TiO 2 -CeO 2 . Disperse 50 mg of TiO 2 -CeO 2 in 50 mL of potassium phosphate buffer solution with pH=6.5, then add 200 uL of 1 uM CYP119 enzyme solution, keep stirring for 20 min, centrifuge to obtain a solid, wash and freeze-dry, namely get the product.
将所得产品用于降解初始浓度为2 mM Cr6+溶液，考查产品的光催化性能。The obtained product was used to degrade the solution with an initial concentration of 2 mM Cr 6+ to examine the photocatalytic performance of the product.
实施例2Example 2
不断搅拌下将0.2996 g Ce(NO3)3·6H2O溶解于40 mL乙醇；在40 mL乙醇和20 mLH2O混和液中加入704 ul钛酸正丁酯；将上述两种溶液混和，通过超声分散均匀，再转入水热反应釜于120℃水热反应48 h后，将所得固体离心分离，干燥后于450℃煅烧5 h，得到TiO2-CeO2。将50 mg TiO2-CeO2分散于50 mL pH=6.5的磷酸钾缓冲溶液中，再加入200 uL的1 uM的CYP119酶溶液，持续搅拌20 min，离心分离得到固体，洗涤后冷冻干燥，即得到产品。Dissolve 0.2996 g Ce(NO 3 ) 3 6H 2 O in 40 mL of ethanol under constant stirring; add 704 ul of n-butyl titanate to the mixture of 40 mL of ethanol and 20 mL of H 2 O; mix the above two solutions, Uniformly dispersed by ultrasonic, then transferred to a hydrothermal reaction kettle for hydrothermal reaction at 120°C for 48 hours, the obtained solid was centrifuged, dried and calcined at 450°C for 5 hours to obtain TiO 2 -CeO 2 . Disperse 50 mg of TiO 2 -CeO 2 in 50 mL of potassium phosphate buffer solution with pH=6.5, then add 200 uL of 1 uM CYP119 enzyme solution, keep stirring for 20 min, centrifuge to obtain a solid, wash and freeze-dry, namely get the product.
将所得产品用于降解初始浓度为2 mM Cr6+溶液，考查产品的光催化性能。The obtained product was used to degrade the solution with an initial concentration of 2 mM Cr 6+ to examine the photocatalytic performance of the product.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1796552A (en) * | 2004-12-29 | 2006-07-05 | 华东理工大学 | Method for preparing porous material in lumpy in use for biological zymophore |
| CN101775387A (en) * | 2010-03-23 | 2010-07-14 | 南京工业大学 | Mesoporous titanium dioxide immobilized enzyme and preparation method and application thereof |
| KR20100125845A (en) * | 2009-05-22 | 2010-12-01 | 전남대학교산학협력단 | Pretreatment method of lignocellulosic biomass using photocatalyst and production method of bioethanol using same |
| RU2416644C1 (en) * | 2009-11-17 | 2011-04-20 | Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации | Photobiocatalyst for producing reduced forms of nicotinamide coenzymes nadh or nadph and photocatalytic method for producing nadh or nadph |
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|---|---|---|---|---|
| CN1796552A (en) * | 2004-12-29 | 2006-07-05 | 华东理工大学 | Method for preparing porous material in lumpy in use for biological zymophore |
| KR20100125845A (en) * | 2009-05-22 | 2010-12-01 | 전남대학교산학협력단 | Pretreatment method of lignocellulosic biomass using photocatalyst and production method of bioethanol using same |
| RU2416644C1 (en) * | 2009-11-17 | 2011-04-20 | Российская Федерация, От Имени Которой Выступает Министерство Образования И Науки Российской Федерации | Photobiocatalyst for producing reduced forms of nicotinamide coenzymes nadh or nadph and photocatalytic method for producing nadh or nadph |
| CN101775387A (en) * | 2010-03-23 | 2010-07-14 | 南京工业大学 | Mesoporous titanium dioxide immobilized enzyme and preparation method and application thereof |
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