CN105619510B - A kind of Gelose punch - Google Patents

Abstract

The present invention discloses a kind of Gelose punch, is related to technical field of experiment equipment.Including base, support, limit valve, tooth bar, mobile station, gear, front apron, backboard, side shield, air bag, gauze, agar the storage box, perforating head, screens valve, cutting knife, key and direction switch.By the fine tuning structure and limit valve of rack-and-pinion, can positioned-lifting perforating head, on perforating head cutting knife cutting agar after, the agar that will be cut out with air bag is suctioned out and is stored in box, so as to get the non-through hole of planned depth.Due to non-through hole with the end can be got, experimental error caused by can avoiding adding the liquor sample in hole from being spilt to surrounding from bottom, separately can by quantitative rotation set graduated base get on request fixing hole away from multiple holes.The card punch is easy to operate, cheap, with stronger promotional value.

Description

an agar punch

technical field

The invention relates to the technical field of experimental equipment, in particular to an agar puncher.

Background technique

Many experiments in biology and medicine need to make holes on the agar spread on the surface of glass slides or petri dishes, such as immunodiffusion experiments, drug sensitivity experiments, etc. The impact of the punching quality on the experimental results cannot be ignored, and there is still no ideal and dedicated unified punch.

Most of the existing hole punchers use stainless steel thin-walled tubes or glass tubes to directly insert the agar into the bottom of a glass slide or a culture dish, then take out the agar in the hole, and punch through holes that penetrate to the bottom of the agar. However, when the sample is added to the hole, it may leak from the bottom, affecting the experimental results. Some researchers added melted agar back to the through-holes to form overmolded non-through-holes, which was inconvenient to operate, and sometimes uneven bottoms would also affect the experimental results. Therefore, it is particularly important to be able to punch non-through holes in one molding.

In experiments, such as drug susceptibility experiments, it is often necessary to drill multiple holes according to the specified hole spacing for each medium to meet the requirements of the experiment. Existing punches with a single punching head are not easy to grasp the hole distance when punching holes, and need to measure and mark the bottom of the agar to punch holes in sequence; hole. However, when multiple holes are drilled at the same time, it is easy to cause the agar to swell and crack, and the edge of the agar may be turned up. Therefore, it is also a problem to be solved urgently whether the hole distance can be accurately grasped without damaging the agar when punching holes.

Contents of the invention

Aiming at the deficiencies of the prior art, the invention discloses an agar puncher, which can solve the problems of punching non-through holes and punching holes with precise and fixed distances at one time.

To achieve the above object, the present invention is achieved through the following technical solutions:

An agar puncher, including a base, a bracket, a limit valve, a rack, a mobile platform, a gear, a front baffle, a rear baffle, a side baffle, an air bag, a gauze, an agar storage box, a punching head, a card Position valve, cutter, key and lift switch, the bracket is set on the base and is perpendicular to the base; the rack is set on the bracket by screws; the mobile table is nested on the bracket and the rack, and the rack is engaged with the mobile table; The right side of the mobile table is connected with the agar storage box and the punching head, the gear is connected with the front baffle and the rear baffle, and the lifting switch is connected with the gear through the shaft.

Preferably, the limit valve is arranged on a bracket, and bolts are arranged on the limit valve.

Preferably, the upper end of the punching head is provided with an agar storage box, and the agar storage box is threadedly connected to the mobile platform; the punching head is a double stainless steel tube structure, the inner tube is connected to the agar storage box, and the outer tube is sleeved on the inner tube. The nozzle of the outer pipe is provided with a cutter; the middle part of the outer pipe is provided with a groove, and the middle part of the inner pipe is provided with a raised locking valve, which can move in the groove.

Preferably, the cutter is made of fine steel wire.

Preferably, the upper end of the agar storage box has a small hole and is connected with an air bag with a one-way valve, and the air bag forms a closed structure with the agar storage box and the punching head; gauze is arranged inside the agar storage box.

Preferably, there are scales on the base.

The invention discloses an agar puncher, which can punch non-through holes, eliminate the leakage of liquid samples at the bottom of the through holes, and improve the accuracy of experimental results; when punching holes, refer to the scale on the base to accurately control the hole distance Multiple holes are punched in sequence, which saves the trouble of measuring marks before punching, further improves the accuracy of the experimental results, saves time and effort; the hole punch is equipped with an agar storage box, and the punched agar is sucked into the punch box by negative pressure It saves the time to clean up the excised agar after each punching.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only These are some embodiments of the present invention. Those skilled in the art can also obtain other drawings based on these drawings without creative work.

Fig. 1 is the front view structure schematic diagram of the present invention;

Fig. 2 is a top view structural representation of the present invention;

Fig. 3 is the structural representation of limit valve among the present invention;

Fig. 4 is the structural representation of punching head among the present invention;

Fig. 5 is a structural schematic diagram of the base scale in the present invention;

In the figure 1. Base, 2. Bracket, 3. Limit valve, 4. Rack, 5. Mobile platform, 6. Gear, 7. Front baffle, 8. Airbag, 9. Gauze, 10. Agar storage box , 11, side baffle, 12, perforating head, 13, clamping valve, 14, cutter, 15, tailgate, 16, key, 17, lifting switch.

detailed description

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

Referring to Fig. 1 and Fig. 2, an agar puncher includes: base 1, bracket 2, limit valve 3, rack 4, mobile platform 5, gear 6, front baffle 7, rear baffle 15, side baffles Plate 11, air bag 8, gauze 9, agar storage box 10, punching head 12, stop valve 13, cutter 14, key 16, lifting switch 17 etc. are formed. The bracket 2 is welded on the base 1 and is perpendicular to the base 1. The rack 4 is attached to the bracket 2 by screws. The mobile platform 5 is nested on the bracket 2 and the rack 4 , and the rack 4 is designed in a special shape and engages with the mobile platform 5 , so that the mobile platform 5 can complete the lifting process on the bracket 2 . The right side of the mobile table 5 is connected to the agar storage box 10 and the punching head 12, the gear 6 is connected to the rear baffle 15 through the front baffle 7, the lift switch 16 is connected to the gear 6 through the shaft, and the punching head 12 can be controlled by turning the lift switch 17 up and down.

Referring to Figure 1, Figure 2 and Figure 3, the limit valve 3 is nested on the bracket 2 as shown in the figure, and the limit valve 3 is provided with bolts, which can be used to fix the position of the limit valve 3. When the mobile platform 5 descends to the position of the limit valve 3, it stops descending, thereby controlling the lowest position where the punching head 12 descends. In one experiment, the depth of the drilled holes can be made the same through the limit valve 3, and the operation is simple and quick.

Referring to Fig. 1 and Fig. 4, the upper end of the punching head 12 is provided with an agar storage box 10, and the agar storage box 10 is screwed to the mobile platform 5. The punching head 12 is a double stainless steel pipe structure, the inner pipe is connected to the agar storage box 10, the outer pipe is sheathed on the inner pipe, and the mouth of the outer pipe is provided with a cutter 14. And the inner and outer tubes are close in diameter, and the gap between the inner and outer tubes is negligible. A groove is arranged in the middle of the outer tube, and a locking valve is arranged in the middle of the inner tube. The locking valve can move in the groove, so that the outer tube can rotate without falling off. When the punching head 12 enters the agar to a suitable depth, the outer tube is rotated, and the cutter 14 can cut off the agar in the tube to achieve the purpose of cutting. Since the single-layer steel pipe is inserted into the agar, when the thickness of the agar in the pipe accounts for a relatively large proportion of the total thickness, rotating the steel pipe will cause the agar in the pipe to drive the rotation of the remaining small part of the agar that has not entered the pipe, thereby destroying the structure of the agar that has not entered the pipe. Therefore, the present invention Adopt the structure that the inner tube does not move and the outer tube rotates.

The upper end of the agar storage box 10 has a small hole and is connected with the air bag 8 with a one-way valve. The air bag 8 forms a closed structure with the agar storage box 10 and the punching head 12 . After the agar entering the tube is cut off, squeeze the air bag 8 to suck the cut agar into the agar storage box 10 . The agar storage box 10 is provided with gauze 9, which can prevent the cut agar from entering the air bag 8.

Referring to Fig. 5, there are scales marked on the base 1, and the intersection of the two columns of scales is the position of punching. Taking the hole distance as 3cm as an example, first find the center position of the agar culture dish through the scale, and drill the first hole. Then move the Petri dish 3 cm to the right and make a second hole. According to the number of holes you want to punch, take the first hole as the center and rotate the second hole clockwise to the position corresponding to the number of holes. If drilling 6 holes, then rotate clockwise 360°/6=60°, which is the corresponding "6" position. In the same way, if you drill 7 holes, then rotate clockwise 360°/7≈51.43°, which is the corresponding "7 position". Drill the third hole, then still centering on the first hole, turn the third hole clockwise into position. And so on, punch all the holes.

In summary, the present invention discloses a kind of agar puncher, through the fine-tuning structure of the rack and pinion and the limit valve, the punching head can be positioned and lifted, and after the cutting knife on the punching head cuts the agar, the cut out is cut with an air bag. The agar is aspirated and stored in the box, thereby punching non-through holes of the planned depth. Since the bottomed non-penetrating hole can be drilled, it can avoid the experimental error caused by the leakage of the liquid sample added into the hole from the bottom to the surrounding. In addition, multiple holes with fixed hole spacing can be punched as required by quantitatively rotating the base with scales. . The hole puncher is easy to operate, low in price, and has strong promotional value.

It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply that there is a relationship between these entities or operations. any such actual relationship or order exists between them. Furthermore, the term "comprises", "comprises" or any other variation thereof is intended to cover a non-exclusive inclusion such that a process, method, article, or apparatus comprising a set of elements includes not only those elements, but also includes elements not expressly listed. other elements of or also include elements inherent in such a process, method, article, or device. Without further limitations, an element defined by the phrase "comprising a ..." does not exclude the presence of additional identical elements in the process, method, article or apparatus comprising said element.

The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: it can still be described in the foregoing embodiments Modifications are made to the recorded technical solutions, or equivalent replacements are made to some of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (3)

1. a kind of Gelose punch, including base, support, limit valve, tooth bar, mobile station, gear, front apron, backboard, side block Plate, air bag, gauze, agar the storage box, perforating head, screens valve, cutting knife, key and direction switch, it is characterised in that：Support is arranged on It is on base and vertical with base；Tooth bar is arranged on support by screw；Mobile station is nested on support and tooth bar, tooth bar with Mobile station engages；The connection of mobile station right side agar the storage box and perforating head, gear, direction switch are connected by front apron backboard It is connected with gear by axle；The perforating head upper end is provided with agar the storage box, and agar the storage box is threadedly attached in mobile station On；Perforating head is double stainless steel tube structures, and inner tube connects agar the storage box, and on inner pipe, the outer tube mouth of pipe is provided with cutting knife to outer tube sleeve； Outer tube middle part is provided with conduit, and inner tube middle part is provided with the screens valve of projection, and screens valve can be moved in conduit；The agar storage Box upper end is provided with aperture and is connected with the air bag with check valve, and air bag forms closed knot with agar the storage box and perforating head Structure；Gauze is provided with agar the storage box；There is scale on the base.

2. Gelose punch as claimed in claim 1, it is characterised in that：The limit valve is arranged on support, is set on limit valve There is bolt.

3. Gelose punch as claimed in claim 1, it is characterised in that：The cutting knife uses finer wire material.