CN1055966C - Production of glossy ganoderma health drink by submerged fermentation method - Google Patents
Production of glossy ganoderma health drink by submerged fermentation method Download PDFInfo
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- CN1055966C CN1055966C CN96119695A CN96119695A CN1055966C CN 1055966 C CN1055966 C CN 1055966C CN 96119695 A CN96119695 A CN 96119695A CN 96119695 A CN96119695 A CN 96119695A CN 1055966 C CN1055966 C CN 1055966C
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Abstract
The present invention discloses a method for producing a ganoderma lucidum health-care beverage with a deep fermentation method. The method comprises the procedures: ganoderma lucidum S-W119 is used as strains, and potato class extract juice, bean class extract juice, corn milk, electrolytes which contain sodium and magnesium, glucose and other raw material are used as culture media; the strains are cultivated by a slant surface, and are deeply fermented by a secondary triangular shake flask, a fermentor whose capacity is 50 liters, a fermentor whose capacity is 500 liters and a fermentor whose capacity is 5000 liters for four to six days, and the product is obtained. The method has the advantages of simplicity and short production period. The present invention realizes synchronous culture, long hyphostroma potential and regular and uniform molding. Fermented products are pure natural, and have good mouth feel and high medicinal and nutritive values. The method has a high industrialization degree, so the present invention has great application prospects.
Description
The present invention relates to a kind of method of producing glossy ganoderma health beverage, specifically is exactly to use production of glossy ganoderma health drink by submerged fermentation method.It belongs to the microbial engineering field, also belongs to health product technology field.
Glossy ganoderma also claims Ganoderma Lucidum, red spirit, and formal name used at school is Ganoderma Lucidum Karst, is a kind of fungi, has high pharmaceutical use, be described as " elixir ", it is warm in nature, flavor is sweet, the beneficial vital essence of function, strengthening the bones and muscles, cures mainly diseases such as palpitation and insomnia, forgetful, spiritlessness and weakness.
Because glossy ganoderma widely is used as medicine, and wild Ganoderma is special to the growing environment requirement, and its output is few, must seek a kind of alternate resources.So, as far back as the '30s just the someone carried out the research of artificial culture glossy ganoderma.Yet, no matter be Wild Ganoderma or tame Ganoderma sporophore, its fibrosis height, bitter taste is dense, reaches 3～6 months vegetative period, and interfering factors is many, the scale operation difficulty, thereby greatly limited the exploitation and the use of ganoderma lucidum prod.In recent years, people have carried out the research of deep glossy ganoderma fermenting method, and this method is to cultivate in substratum with a kind of Ganderma lucidum strain is first, control certain condition fermentation and obtain mycelium.Used substratum is mainly starch juice, but its condition of cultivating, fermenting is difficult to control, and mycelial growing way and shaping are irregular, and effect is undesirable.Because of this method still is in the laboratory study stage at present, large-scale production yet there are no report.
The objective of the invention is to: in order to overcome the deficiencies in the prior art, the present invention will select suitable bacterial classification and substratum, and best cultivation and fermentation condition is fermented by the glossy ganoderma slant culture.Characteristics such as this method should possess the technology simple and stable, and is with short production cycle, and mycelium growing way and shaping are neatly even, but also should be suitable for industrial-scale production, products therefrom can be used as nourishing drink and directly uses.
For realizing that the technical measures that purpose of the present invention is taked are:
1. the bacterial classification of the present invention's selection is a kind of Ganderma lucidum (Ganoderma Lucidum) S W119, by separating from the wild Ganoderma sporophore of adopting and getting.This bacterial classification is submitted the preservation of specified depositary institution on August 1st, 1994, and its depositary institution and deposit number are: Chinese typical culture collection center C CTCC No:M94051.
2. by above-mentioned bacterial classification method with production of glossy ganoderma health drink by submerged fermentation method: bacterial classification in substratum through slant culture, again successively through the 500ml triangle shake bottle, the 5000ml triangle shakes bottle, 50 liters of fermentor tanks, 500 liters of fermentor tanks, 5000 liters of fermentor tank submerged fermentations, the transparent limpid fermented liquid of gained is glossy ganoderma health beverage.Feed sterile air during the fermentation, air flow is 1: 1, and the cultivation and fermentation temperature is 25～33 ℃, 4～6 days time.
Substratum of the present invention is the full water-soluble material, and it consists of: potato class diffusion juice is as cultivating nutrient: 10～30%; The beans diffusion juice is as growth stimulant 2～10%; As growth regulator 0.01～0.08%, corn steep liquor is 0.1～0.5% with the inorganic salt ionogen that contains potassium, magnesium, and glucose is 1～3%, and all the other are moisture content.
In the above-mentioned substratum, with KH
2PO
4And MgSO
4As growth regulator the best, preferred substratum consists of, potato diffusion juice 20%, bean sprouts diffusion juice 5%, KH
2PO
4+ MgSO
4Be 0.04%, corn steep liquor 0.1%, glucose 2%, all the other are moisture content.
Compared with the prior art, the beneficial effect that adopts technical measures of the present invention and reached:
1. the processing method of production of glossy ganoderma health drink by submerged fermentation method of the present invention is simple, and is with short production cycle, is 5～6 days only, and production cost is low, and the industrialization degree height, can realize plant-scale production.
2. the condition of present method is controlled easily, and realizes synchronized culture, and mycelial growing way is neatly even, and mostly is pearl shape, has improved the mycelia amount in the unit volume.Substratum is mainly middlebrow crop products, conveniently is easy to get.
3. be all-natural product by present method products obtained therefrom, do not contain any Chemical Preservative, contain the necessary amino acid of ganoderan and human body and some trace elements.Total sugar content is～18.3mg/ml that polysaccharide is～5.5mg/ml.This product does not have bitter taste, salubrious tasty and refreshing, sweet lubricious larynx.Hubei Province Food Hygiene Surveillance check the toxicological test in first and second stage of this product is shown: this product safety non-toxic.
The present invention is further illustrated below in conjunction with concrete embodiment:
The bacterial classification of selecting for use is glossy ganoderma S-W119 (CCTCC No:M94051), and substratum consists of: potato diffusion juice 20%, bean sprouts diffusion juice 5%, KH
2PO
4+ MgSO
4Be 0.04%, corn steep liquor 0.1%, glucose 2%, all the other are moisture content.
With bacterial classification in above-mentioned substratum through slant culture, then successively through the 500ml triangle shake bottle, the 5000ml triangle shakes bottle, 50 liters of fermentor tanks, 500 liters of fermentor tanks and 5000 liters of fermentor tank submerged fermentations.Temperature is controlled at 28 ± 2 ℃ during fermentation, and feeds sterile air, and air flow is 1: 1.Put jar after 5 days and promptly get transparent limpid fermented liquid, mycelium is a pearl shape sphere, and size is even, and this fermented liquid is glossy ganoderma health beverage, can directly drink.
Claims (3)
1. a ganoderma strain capable (Ganoderma Lucidum) S-W119, it is characterized in that: this bacterial strain is to be preserved in Chinese typical culture center (CCTCC) on August 1st, 1994, and deposit number is the microorganism strains of M94051.
2. method with production of glossy ganoderma health drink by submerged fermentation method, this method is first through slant culture in substratum by ganoderma strain capable at 25～33 ℃, again successively through the 500ml triangle shake bottle, the 5000ml triangle shakes bottle, 50 liters of fermentor tanks, 500 liters of fermentor tanks and 5000 liters of fermentor tank submerged fermentations and promptly got glossy ganoderma health beverage in 5～6 days and it is characterized in that: described ganoderma strain capable is the bacterial strain that is preserved in Chinese typical culture center, numbers CCTCC NO:M94051, and the weight percent of described substratum consists of:
Potato diffusion juice 10～30%
Bean sprouts diffusion juice 2～10%
The ionogen 0.01～0.08% that contains sodium, magnesium
Corn steep liquor 0.1～0.5%
Glucose 1～3%
All the other are moisture content.
3. method according to claim 2 is characterized in that the weight percent of described substratum consists of:
Potato diffusion juice 20%
Bean sprouts diffusion juice 5%
KH
2PO
4And MgSO
40.04%
Corn steep liquor 0.1%
Glucose 2%
All the other are moisture content.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119695A CN1055966C (en) | 1996-12-04 | 1996-12-04 | Production of glossy ganoderma health drink by submerged fermentation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119695A CN1055966C (en) | 1996-12-04 | 1996-12-04 | Production of glossy ganoderma health drink by submerged fermentation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1163930A CN1163930A (en) | 1997-11-05 |
| CN1055966C true CN1055966C (en) | 2000-08-30 |
Family
ID=5125929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96119695A Expired - Fee Related CN1055966C (en) | 1996-12-04 | 1996-12-04 | Production of glossy ganoderma health drink by submerged fermentation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1055966C (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100387704C (en) * | 2005-04-21 | 2008-05-14 | 中国农业大学 | Ganoderma lucidum fungus capable of high-yield glycopeptide complex and its mutagenesis selection method and application |
| CN1298259C (en) * | 2005-10-13 | 2007-02-07 | 南京农业大学 | Method for producing fruit juice of germinant unpolished rice and ganoderma lucidum, and production |
| CN102771858A (en) * | 2012-07-20 | 2012-11-14 | 黄晓青 | Ganoderma sinensis health drink prepared by liquid-submerged fermentation and preparation method of Ganoderma sinensis health drink |
| CN102771769B (en) * | 2012-07-20 | 2015-05-27 | 北京厚文知识产权顾问有限公司 | Ganoderma sinensis health product and preparation method thereof |
| CN102835624B (en) * | 2012-09-21 | 2013-09-11 | 尹新波 | Ganoderan noodle and production method thereof |
| CN102894447B (en) * | 2012-11-02 | 2013-08-14 | 尹新波 | Ganoderma lucidum polysaccharide health-care drink and production method thereof |
| CN103081723A (en) * | 2013-02-17 | 2013-05-08 | 哈尔滨伟平科技开发有限公司 | Preparation method for Gloeostereum incarnatum powder |
| CN103549582B (en) * | 2013-10-25 | 2015-08-12 | 湖北师范学院 | A kind of lucid ganoderma health-care beverage and preparation method thereof |
| CN105105236A (en) * | 2015-07-09 | 2015-12-02 | 淮阴师范学院 | Burdock fermented composite polysaccharide beverage |
| CN108934769B (en) * | 2018-06-26 | 2021-01-29 | 广东省微生物研究所（广东省微生物分析检测中心） | Submerged fermentation medium for fomes ruditapes, and preparation and application methods thereof |
-
1996
- 1996-12-04 CN CN96119695A patent/CN1055966C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1163930A (en) | 1997-11-05 |
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Granted publication date: 20000830 |