CN105594845A - Novel cassava color-protection fresh-keeping agent, and fresh keeping method - Google Patents
Novel cassava color-protection fresh-keeping agent, and fresh keeping method Download PDFInfo
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Abstract
本发明涉及一种木薯护色保鲜剂及其保鲜方法,所述保鲜剂的组成中包括去离子重蒸水和下述三种组分中的两种或三种:(A)壳聚糖、海藻酸钠和魔芋葡甘聚糖中的一种或两种或三种;(B)柠檬酸和抗坏血酸中的一种或两种;(C)氯化钙。采用本发明的木薯护色保鲜剂进行保鲜,能有效防止木薯收获后褐变、腐烂,保持木薯良好贮藏品质,延长贮藏保鲜时间。
The present invention relates to a kind of cassava color-protecting antistaling agent and fresh-keeping method thereof, and the composition of described antistaling agent comprises deionized redistilled water and two or three kinds in following three kinds of components: (A) chitosan, One or two or three of sodium alginate and konjac glucomannan; (B) one or two of citric acid and ascorbic acid; (C) calcium chloride. The cassava color-preserving and fresh-keeping agent of the invention is used for fresh-keeping, which can effectively prevent cassava from browning and rotting after harvesting, maintain good storage quality of cassava, and prolong the storage and fresh-keeping time.
Description
技术领域technical field
本发明涉及一种保鲜剂及其在木薯保鲜中的应用,涉及食品的保鲜领域。The invention relates to a fresh-keeping agent and its application in cassava fresh-keeping, and relates to the field of food fresh-keeping.
背景技术Background technique
木薯(ManihotesculentaCrantz)属于大戟科(Euphorbiaceae)木薯属植物,是世界三大薯类(木薯、马铃薯、甘薯)之一。其块根富含淀粉,是热带亚热带地区重要的生物质能源物质。由于木薯鲜薯组织脆嫩,水分含量高,在加工及贮藏过程中易出现“采后生理性变质”,导致块根失水、褐化,腐烂,鲜木薯贮存期极短(1-2天),从而制约了木薯大量上市供应及产后综合利用,严重影响了其商品价值。Cassava (Manihotesculenta Crantz) belongs to Euphorbiaceae (Euphorbiaceae) Cassava plant, is one of the world's three largest potato (cassava, potato, sweet potato). Its root tuber is rich in starch and is an important biomass energy material in tropical and subtropical regions. Due to the crisp and tender tissues of fresh cassava and high water content, "post-harvest physiological deterioration" is prone to occur during processing and storage, resulting in dehydration, browning, and rot of roots, and the storage period of fresh cassava is extremely short (1-2 days). , thereby restricting the supply of cassava on the market in large quantities and its post-production comprehensive utilization, seriously affecting its commodity value.
木薯的传统贮藏保鲜方法主要有封蜡、沙埋、沟壕保存等,可延长木薯保质期至2个月,但经过储藏后木薯块根易褐变、变味,无法食用。近年来,由生物高分子等材料制成的保鲜材料如壳聚糖膜、淀粉膜等,由于其无毒、可降解和生物相容性高等特点,被广泛应用到果蔬贮藏领域,显著提高了果蔬储藏和保鲜效果(“不同涂膜处理对马铃薯货架期品质的影响”,食品与机械,2011,27(3)122~124),而其在木薯鲜薯的储藏和保鲜中却鲜见应用。The traditional storage and preservation methods of cassava mainly include sealing wax, sand burial, trench preservation, etc., which can extend the shelf life of cassava to 2 months, but after storage, cassava roots tend to brown and change taste, making them inedible. In recent years, fresh-keeping materials made of materials such as biopolymers, such as chitosan film and starch film, have been widely used in the field of fruit and vegetable storage due to their non-toxic, degradable and high biocompatibility characteristics, which have significantly improved Fruit and vegetable storage and fresh-keeping effects (“Effects of different coating treatments on the shelf-life quality of potatoes”, Food and Machinery, 2011, 27(3) 122-124), but it is rarely used in the storage and fresh-keeping of fresh cassava .
发明内容Contents of the invention
本发明的目的在于弥补现有技术的不足,提供一种新型木薯护色保鲜剂及其保鲜方法,能有效防止木薯收获后褐变、腐烂,保持木薯良好贮藏品质,延长贮藏保鲜时间。使用本发明的木薯护色保鲜剂对经热处理的木薯涂膜保鲜,能够有效延长木薯贮藏期,保持其在贮藏中的营养价值,从而有效提高木薯的商业价值,延伸木薯产业链。The purpose of the present invention is to make up for the deficiencies of the prior art, and provide a novel cassava color-protecting and fresh-keeping agent and its fresh-keeping method, which can effectively prevent cassava from browning and rotting after harvesting, maintain good storage quality of cassava, and prolong the storage and fresh-keeping time. Using the cassava color-protecting and antistaling agent of the present invention to preserve the freshness of the heat-treated cassava coating film can effectively prolong the cassava storage period and maintain its nutritional value during storage, thereby effectively increasing the commercial value of the cassava and extending the cassava industry chain.
为实现上述目的,本发明提供如下技术方案:To achieve the above object, the present invention provides the following technical solutions:
一种木薯护色保鲜剂,其组成中包括去离子重蒸水和下述三种组分中的两种或三种:A kind of cassava color-protecting and antistaling agent comprises deionized redistilled water and two or three of the following three components in its composition:
(A)壳聚糖、海藻酸钠和魔芋葡甘聚糖中的一种或两种或三种;(A) one or two or three of chitosan, sodium alginate and konjac glucomannan;
(B)柠檬酸和抗坏血酸中的一种或两种;(B) one or both of citric acid and ascorbic acid;
(C)氯化钙。(C) calcium chloride.
其中,所述保鲜剂中不含防腐剂。Wherein, the preservative does not contain preservatives.
其中,所述木薯护色保鲜剂的组成为去离子重蒸水和下述三种组分中的两种或三种:Wherein, the composition of described cassava color-protecting and antistaling agent is deionized redistilled water and two or three kinds in following three kinds of components:
(A)壳聚糖、海藻酸钠和魔芋葡甘聚糖中的一种或两种或三种;(A) one or two or three of chitosan, sodium alginate and konjac glucomannan;
(B)柠檬酸和抗坏血酸中的一种或两种;(B) one or both of citric acid and ascorbic acid;
(C)氯化钙。(C) calcium chloride.
在本发明的一个实施方案中,所述保鲜剂的组成为去离子重蒸水、壳聚糖、抗坏血酸和氯化钙;或者,所述保鲜剂的组成为去离子重蒸水、海藻酸钠、抗坏血酸和氯化钙;或者,所述保鲜剂的组成为去离子重蒸水、壳聚糖、柠檬酸和氯化钙;或者,所述保鲜剂的组成为去离子重蒸水、海藻酸钠、柠檬酸和氯化钙;或者,所述保鲜剂的组成为去离子重蒸水、魔芋葡甘聚糖、柠檬酸和氯化钙;或者所述保鲜剂的组成为去离子重蒸水、魔芋葡甘聚糖、抗坏血酸和氯化钙。In one embodiment of the present invention, the composition of the preservative is deionized redistilled water, chitosan, ascorbic acid and calcium chloride; or, the composition of the preservative is deionized redistilled water, sodium alginate , ascorbic acid and calcium chloride; or, the composition of the preservative is deionized redistilled water, chitosan, citric acid and calcium chloride; Sodium, citric acid and calcium chloride; Or, the composition of described antistaling agent is deionized heavy distilled water, konjac glucomannan, citric acid and calcium chloride; Perhaps the composition of described antistaling agent is deionized heavy distilled water , Konjac Glucomannan, Ascorbic Acid and Calcium Chloride.
其中,所述(A)、(B)和(C)组分的两种或三种的总和的质量百分比为1~10wt%,优选1~6wt%。Wherein, the mass percentage of the sum of two or three of the (A), (B) and (C) components is 1-10 wt%, preferably 1-6 wt%.
其中,所述(A)组分的质量百分比为1~3wt%,优选1.5~2wt%。所述(B)组分的质量百分比为0.1~2wt%,优选0.15~0.5wt%。所述(C)组分的质量百分比为0.1~0.5wt%,优选0.2~0.4wt%。Wherein, the mass percentage of the (A) component is 1-3 wt%, preferably 1.5-2 wt%. The mass percentage of the (B) component is 0.1-2wt%, preferably 0.15-0.5wt%. The mass percentage of the (C) component is 0.1-0.5 wt%, preferably 0.2-0.4 wt%.
其中,优选地,所述木薯护色保鲜剂的组分和质量百分比为:Wherein, preferably, the component and mass percent of described cassava color-protecting and antistaling agent are:
(A)壳聚糖、海藻酸钠和魔芋葡甘聚糖中的一种或两种或三种,1~3wt%,优选1.5~2wt%;(A) one or two or three of chitosan, sodium alginate and konjac glucomannan, 1-3wt%, preferably 1.5-2wt%;
(B)柠檬酸和抗坏血酸中的一种或两种,0.1~2wt%,优选0.15~0.5wt%;(B) one or both of citric acid and ascorbic acid, 0.1-2wt%, preferably 0.15-0.5wt%;
(C)氯化钙,0.1~0.5wt%,优选0.2~0.4wt%;(C) calcium chloride, 0.1~0.5wt%, preferably 0.2~0.4wt%;
余量,去离子重蒸水。The remainder was deionized redistilled water.
本发明还提供如下技术方案:The present invention also provides the following technical solutions:
一种木薯的保鲜加工方法,所述方法中包括热处理步骤、上述的木薯护色保鲜剂的处理步骤和真空包装步骤。A fresh-keeping processing method of cassava, comprising a heat treatment step, a treatment step of the above-mentioned cassava color-protecting and fresh-keeping agent and a vacuum packaging step.
具体的,所述方法包括如下工艺流程:原料挑选→清洗→去皮→整形、切分→热处理→上述的木薯护色保鲜剂处理→风干→装袋→真空包装→贮藏。Specifically, the method includes the following technological process: raw material selection→cleaning→peeling→shaping, cutting→heat treatment→treatment with the above-mentioned cassava color-protecting and preservative→air-drying→bagging→vacuum packaging→storage.
所述原料挑选步骤具体为:挑选无机械损伤、无病虫害、品质好的新鲜木薯作为原料。The step of selecting raw materials specifically includes: selecting fresh cassava with no mechanical damage, no pests and diseases, and good quality as the raw material.
所述的清洗、去皮和整形、切分步骤具体为:将原料去除泥沙、用流水清洗、去皮后,切分成适合食用所需形状。The steps of cleaning, peeling, shaping and cutting are as follows: removing sediment from the raw materials, washing with running water, peeling, and cutting into shapes suitable for consumption.
所述的热处理步骤具体为:将木薯放入45-80℃的热水中烫漂2~10min,取出后去除表面水分。The heat treatment step specifically includes: putting the cassava into hot water at 45-80°C for 2-10 minutes, and removing the surface moisture after taking it out.
所述的保鲜剂处理、风干步骤具体为:将经热处理后的木薯置于所述的木薯护色保鲜剂中,浸泡5~10min,取出后风干。The preservative treatment and air-drying steps specifically include: placing the heat-treated cassava in the cassava color-protecting preservative, soaking for 5-10 minutes, taking it out and air-drying.
所述的装袋、真空包装和贮藏步骤具体为:将木薯置于包装袋中,真空包装,置于15-30℃,优选25-28℃条件下贮藏。The steps of bagging, vacuum packaging and storage specifically include: placing the cassava in a packaging bag, vacuum packaging, and storing at 15-30°C, preferably 25-28°C.
其中,所述整形、切分步骤中的适合食用所需的形状为块、条或片;其中,块的长度为2~5cm,厚度为2~5cm;条的长度为8~10cm,厚度为2~3cm;片的厚度为2~4cm。Wherein, the shape suitable for eating in the described shaping and cutting steps is a block, bar or sheet; wherein, the length of the block is 2-5 cm, and the thickness is 2-5 cm; the length of the bar is 8-10 cm, and the thickness is 2 to 3 cm; the thickness of the sheet is 2 to 4 cm.
其中,所述热处理步骤中的温度优选为55~70℃,时间优选为4~6min。Wherein, the temperature in the heat treatment step is preferably 55-70° C., and the time is preferably 4-6 minutes.
其中,所述热处理步骤中必须完全去除木薯表面的水分。Wherein, the moisture on the cassava surface must be completely removed in the heat treatment step.
其中,保鲜剂处理、风干步骤中的浸泡时间优选为3~10min,木薯与所述保鲜剂的体积比为1:2~5(优选1:3)。Wherein, the soaking time in the preservative treatment and air-drying steps is preferably 3-10 minutes, and the volume ratio of cassava to the preservative is 1:2-5 (preferably 1:3).
其中,包装步骤中的包装袋材料为聚乙烯(PE)、聚丙烯(PP)或铝箔(PT)。Wherein, the packaging bag material in the packaging step is polyethylene (PE), polypropylene (PP) or aluminum foil (PT).
其中,所述包装步骤中的真空度为-0.06MPa~-0.1MPa。Wherein, the degree of vacuum in the packaging step is -0.06MPa˜-0.1MPa.
本发明的一种木薯护色保鲜剂及其保鲜方法,具有以下有益效果:A kind of cassava color-protecting antistaling agent and fresh-keeping method thereof of the present invention have following beneficial effects:
该方法所生产的木薯为半成熟化产品,既耐贮藏,又方便消费者追求方便、快捷的生活需要。The cassava produced by the method is a semi-ripe product, which is not only resistant to storage, but also convenient for consumers to pursue convenient and fast life needs.
本发明利用物理方法与可食用多糖复合保鲜剂结合抑制木薯的褐变,不使用任何防腐剂,既达到了防止产品变质的目的,又保证了食品安全。The present invention combines physical methods with edible polysaccharide composite antistaling agents to inhibit browning of cassava without using any preservatives, thereby not only achieving the purpose of preventing product deterioration, but also ensuring food safety.
该发明生产的木薯在常温贮藏10天以上,其色泽、风味和营养品质均保持正常,既方便了贮运,又利于提升木薯的商业价值。The cassava produced by the invention is stored at room temperature for more than 10 days, and its color, flavor and nutritional quality are all kept normal, which not only facilitates storage and transportation, but also helps to increase the commercial value of the cassava.
附图说明:Description of drawings:
图1本发明之一种新型木薯护色保鲜方法工艺流程图Fig. 1 process flow chart of a novel cassava color-protecting and fresh-keeping method of the present invention
图2保鲜剂对木薯失重率的影响;The influence of Fig. 2 preservative on cassava weight loss rate;
图3保鲜剂对木薯硬度的影响;The influence of Fig. 3 preservative on cassava hardness;
图4保鲜剂对木薯色泽的影响;The influence of Fig. 4 preservative on cassava color and luster;
图5保鲜剂对木薯淀粉含量的影响;The influence of Fig. 5 preservative on cassava starch content;
图6保鲜剂对木薯可溶性固形物(TSS)含量的影响;The influence of Fig. 6 preservative on cassava soluble solids (TSS) content;
图7保鲜剂对木薯SOD酶活性的影响;The influence of Fig. 7 preservative on cassava SOD enzyme activity;
图8保鲜剂对木薯POD酶活性的影响;The influence of Figure 8 preservative on cassava POD enzyme activity;
图9保鲜剂对木薯总酚含量的影响。Figure 9 The effect of preservatives on the total phenolic content of cassava.
具体实施方式detailed description
以下通过特定的具体实例说明本发明的实施方式,本领域技术人员可由本说明书所揭露的内容轻易地了解本发明的其他优点与功效。本发明还可以通过不同的具体实施方式加以实施或应用,本说明书中的各项细节也可以基于不同方面的应用,在没有背离本发明的精神下进行各种修饰或改变。Embodiments of the present invention are described below through specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through different specific implementation modes, and various modifications or changes can be made to the details in this specification based on applications in different aspects without departing from the spirit of the present invention.
海藻酸钠、壳聚糖、魔芋葡甘聚糖、柠檬酸、抗坏血酸、氯化钙为食品级,磷酸氢二钠、硫代巴比妥酸、过氧化氢等试剂均为国产分析纯,所用蒸馏水为去离子重蒸水。Sodium alginate, chitosan, konjac glucomannan, citric acid, ascorbic acid, and calcium chloride are food grade, and reagents such as disodium hydrogen phosphate, thiobarbituric acid, and hydrogen peroxide are domestic analytically pure. Distilled water is deionized redistilled water.
LFRA1500g型质构仪,美国Brookfield公司;CR-400色差计,日本美能达仪器公司;WYT-4手持折光仪;GH系列高精度恒温水槽,汗诺仪器;电子天平MS104/MS204S梅特勒。LFRA1500g texture analyzer, American Brookfield Company; CR-400 color difference meter, Japan Minolta Instrument Company; WYT-4 handheld refractometer; GH series high-precision constant temperature water tank, Hannuo Instrument; electronic balance MS104/MS204S Mettler.
实施例1Example 1
(1)挑选无机械损伤、无病虫害、品质好的新鲜木薯作为原料;(1) Select fresh cassava with no mechanical damage, no pests and diseases, and good quality as raw material;
(2)将原料去除泥沙、用流水清洗、去皮后,切分成适合食用所需形状,如块、条或片;其中,块的长度为2~5cm,厚度为2~5cm;条的长度为8~10cm,厚度为2~3cm;片的厚度为2~4cm;(2) After the raw material is removed from sediment, washed with running water, and peeled, cut into shapes suitable for eating, such as blocks, strips or slices; wherein, the length of the block is 2-5cm, and the thickness is 2-5cm; The length is 8-10cm, the thickness is 2-3cm; the thickness of the sheet is 2-4cm;
(3)将木薯放入55-60℃的热水中烫漂5min,取出后去除表面水分;(3) Put the cassava into hot water at 55-60°C for 5 minutes, remove the surface moisture after taking it out;
(4)将木薯置于1.5wt%壳聚糖、0.15wt%柠檬酸、0.25wt%氯化钙的去离子重蒸水溶液中(木薯与保鲜剂的体积比1:3),浸泡5min,取出后风干;(4) Cassava is placed in the deionized redistilled aqueous solution of 1.5wt% chitosan, 0.15wt% citric acid, 0.25wt% calcium chloride (the volume ratio of cassava and preservative is 1:3), soak 5min, take out after drying;
(5)将木薯置于聚乙烯袋中,真空包装(真空度-0.06MPa~-0.1MPa)置于25-28℃条件下贮藏。(5) Put the cassava in a polyethylene bag, vacuum pack (vacuum degree -0.06MPa~-0.1MPa) and store at 25-28°C.
贮藏第15天时,在热处理及保鲜剂作用下,木薯块根未发生褐变及腐烂变质,仍保持原有品质,色泽呈淡黄色、口感较好,具有薯香气味。相关质量检测结果如下:失重率为2.0~2.5%,硬度0.75~0.85kg/cm2,L*值43~49,淀粉含量28.5~32.0%,可溶性固形物含量2.35~2.65%,总酚8.5-9.8mg/100g,SOD和POD分别为8.5-9.3U/g·FW·h,21.0-24.0U/g·min。On the 15th day of storage, under the action of heat treatment and preservatives, the cassava root did not undergo browning and rot, and still maintained the original quality, with a light yellow color, good taste, and a sweet potato smell. The relevant quality inspection results are as follows: weight loss rate is 2.0-2.5%, hardness is 0.75-0.85kg/cm 2 , L * value is 43-49, starch content is 28.5-32.0%, soluble solid content is 2.35-2.65%, total phenols are 8.5- 9.8mg/100g, SOD and POD are 8.5-9.3U/g·FW·h, 21.0-24.0U/g·min, respectively.
实施例2Example 2
(1)挑选无机械损伤、无病虫害、品质好的新鲜木薯作为原料;(1) Select fresh cassava with no mechanical damage, no pests and diseases, and good quality as raw material;
(2)将原料去除泥沙、用流水清洗、去皮后,切分成适合食用所需形状,如块、条或片;其中,块的长度为2~5cm,厚度为2~5cm;条的长度为8~10cm,厚度为2~3cm;片的厚度为2~4cm;(2) After the raw material is removed from sediment, washed with running water, and peeled, cut into shapes suitable for eating, such as blocks, strips or slices; wherein, the length of the block is 2-5cm, and the thickness is 2-5cm; The length is 8-10cm, the thickness is 2-3cm; the thickness of the sheet is 2-4cm;
(3)将木薯放入65~70℃的热水中烫漂3min,取出后去除表面水分;(3) Put the cassava into hot water at 65-70°C for 3 minutes, remove the surface moisture after taking it out;
(4)将木薯置于1.5wt%海藻酸钠、0.15wt%抗坏血酸、0.25wt%氯化钙的去离子重蒸水溶液中(木薯与保鲜剂的体积比1:3),浸泡7min,取出后风干;(4) Put cassava in deionized redistilled aqueous solution of 1.5wt% sodium alginate, 0.15wt% ascorbic acid, 0.25wt% calcium chloride (volume ratio of cassava to preservative 1:3), soak for 7min, take out dry;
(5)将木薯置于聚乙烯袋中,真空包装(真空度-0.06MPa~-0.1MPa)置于25-28℃条件下贮藏。(5) Put the cassava in a polyethylene bag, vacuum pack (vacuum degree -0.06MPa~-0.1MPa) and store at 25-28°C.
贮藏第15天时,在热处理及保鲜剂作用下,木薯块根未发生褐变及腐烂变质,仍保持原有品质,色泽呈淡黄色、口感较好,具有薯香气味。相关质量检测结果如下:失重率为2.0~2.5%,硬度0.75~0.85kg/cm2,L*值43~49,淀粉含量28.5~32.0%,可溶性固形物含量2.35~2.65%,总酚8.5-9.8mg/100g,SOD和POD分别为8.5-9.3U/g·FW·h,21.0-24.0U/g·min。On the 15th day of storage, under the action of heat treatment and preservatives, the cassava root did not undergo browning and rot, and still maintained the original quality, with a light yellow color, good taste, and a sweet potato smell. The relevant quality inspection results are as follows: weight loss rate is 2.0-2.5%, hardness is 0.75-0.85kg/cm2, L* value is 43-49, starch content is 28.5-32.0%, soluble solid content is 2.35-2.65%, total phenols are 8.5-9.8 mg/100g, SOD and POD are 8.5-9.3U/g·FW·h, 21.0-24.0U/g·min, respectively.
实施例3-6Example 3-6
其他步骤与实施例1相同,只是其中的保鲜剂的组成为:Other steps are identical with embodiment 1, but the composition of fresh-keeping agent wherein is:
实施例3:1.5wt%海藻酸钠、0.15wt%柠檬酸和0.25wt%氯化钙的去离子重蒸水溶液;Embodiment 3: the deionized redistilled aqueous solution of 1.5wt% sodium alginate, 0.15wt% citric acid and 0.25wt% calcium chloride;
实施例4:1.5wt%魔芋葡甘聚糖、0.15wt%柠檬酸和0.25wt%氯化钙的去离子重蒸水溶液;Embodiment 4: deionized redistilled aqueous solution of 1.5wt% konjac glucomannan, 0.15wt% citric acid and 0.25wt% calcium chloride;
实施例5:1.5wt%壳聚糖、0.15wt%抗坏血酸、0.25wt%氯化钙的去离子重蒸水溶液;Embodiment 5: the deionized redistilled aqueous solution of 1.5wt% chitosan, 0.15wt% ascorbic acid, 0.25wt% calcium chloride;
实施例6:1.5wt%魔芋葡甘聚糖和0.25wt%氯化钙的去离子重蒸水溶液。Example 6: 1.5 wt% konjac glucomannan and 0.25 wt% calcium chloride in deionized redistilled aqueous solution.
实施例3-5在贮藏第15天时,在热处理及保鲜剂作用下,木薯块根未发生褐变及腐烂变质,仍保持原有品质,色泽呈淡黄色、口感较好,具有薯香气味。相关质量检测结果如下:失重率为2.0~2.5%,硬度0.75~0.85kg/cm2,L*值43~49,淀粉含量28.5~32.0%,可溶性固形物含量2.35~2.65%,总酚8.5-9.8mg/100g,SOD和POD分别为8.5-9.3U/g·FW·h,21.0-24.0U/g·min。In Example 3-5, on the 15th day of storage, under the action of heat treatment and preservatives, the cassava tubers did not undergo browning or rot, and still maintained the original quality, with light yellow color, good taste and sweet potato smell. The relevant quality inspection results are as follows: weight loss rate is 2.0-2.5%, hardness is 0.75-0.85kg/cm2, L* value is 43-49, starch content is 28.5-32.0%, soluble solid content is 2.35-2.65%, total phenols are 8.5-9.8 mg/100g, SOD and POD are 8.5-9.3U/g·FW·h, 21.0-24.0U/g·min, respectively.
实施例6在贮藏15天时,L*值为41-43,与实施例3-5相比木薯表面色泽略偏暗,其余指标均相差不大。When Example 6 was stored for 15 days, the L* value was 41-43. Compared with Examples 3-5, the surface color of the cassava was slightly darker, and the other indicators were not much different.
对比例1-2Comparative example 1-2
对比例1:用无菌水替代上述保鲜剂,其他步骤与实施例1同。Comparative example 1: replace the above-mentioned antistaling agent with sterile water, and other steps are the same as in Example 1.
对比例2:用1.5wt%壳聚糖的去离子重蒸水溶液替代上述保鲜剂,其他步骤与实施例1同。Comparative example 2: the deionized redistilled aqueous solution of 1.5wt% chitosan was used to replace the above-mentioned preservative, and other steps were the same as in Example 1.
贮藏第9天时,与实施例1比较,对比例1木薯失重率显著上升,表面色泽变暗、出现萎蔫和起皱现象,贮藏第12天,可溶性固形物含量上升到最高点,淀粉含量下降至27.3%,块根表面开始腐烂变质。贮藏第9天时,与实施例1比较,对比例2木薯失重率,淀粉含量、可溶性固形物含量变化不大,但木薯块根表面色泽变暗,局部有褐变现象产生。On the 9th day of storage, compared with Example 1, the weight loss rate of cassava in Comparative Example 1 increased significantly, the surface color became darker, and wilting and wrinkling occurred. On the 12th day of storage, the soluble solid content rose to the highest point, and the starch content dropped to 27.3%, the root tuber surface began to rot and deteriorate. On the 9th day of storage, compared with Example 1, Comparative Example 2 had little change in cassava weight loss rate, starch content, and soluble solids content, but the surface color of cassava tubers became darker, and local browning occurred.
实施例7相关性能的测定The measurement of embodiment 7 relevant properties
实施例1(保鲜剂A)、实施例2(保鲜剂B)、实施例5(保鲜剂C)和对比例1(CK)中的经热处理后的木薯在相应的保鲜剂中浸泡5min,自然晾干,装入0.01mm聚乙烯袋内真空包装,置于(25±0.5)℃人工气候箱(RH为85%-90%)中贮藏,贮藏期间每3d取样测定一次,每个处理重复3次。The cassava after thermal treatment in embodiment 1 (preservative A), embodiment 2 (preservative B), embodiment 5 (preservative C) and comparative example 1 (CK) soaks 5min in corresponding preservative, naturally Dry it in the air, put it into a 0.01mm polyethylene bag and vacuum pack it, store it in an artificial climate box (RH is 85%-90%) at (25±0.5)°C, take a sample every 3 days during storage, and repeat 3 times for each treatment. Second-rate.
(1)失重率:贮藏前测定木薯的质量,随后每3d测一次,每次重复测量3次,用如下公式计算失重率。(1) Weight loss rate: measure the mass of cassava before storage, then measure it every 3 days, repeat the measurement 3 times each time, and calculate the weight loss rate with the following formula.
(2)硬度:利用质构仪进行测定,沿木薯横切面部位下压进行TPA测试,每面测2个点,重复3次,取平均值。(2) Hardness: use a texture analyzer to measure, press down along the cross-section of cassava for TPA test, measure 2 points on each side, repeat 3 times, and take the average value.
(3)色泽:用分光测色仪测量,每面测定4个点,记录颜色变化情况(L*值);其中,-L*表示向暗色方向变化,+L*表示向亮色方向变化。(3) Color: measure with a spectrophotometer, measure 4 points on each side, and record the color change (L * value); among them, -L * means change to dark color direction, +L * means change to light color direction.
(4)淀粉含量依照GB/T25219-2010测定。(4) Starch content is determined according to GB/T25219-2010.
(5)可溶性固形物(TSS)含量:采用折光仪测定。(5) Soluble solids (TSS) content: measured with a refractometer.
(6)总酚含量:采用Folin-Ciocaileu法测定。(6) Total phenol content: determined by the Folin-Ciocaileu method.
(7)过氧化物酶(POD)、超氧化物歧化酶(SOD)活性:分别采用紫外吸收法及氮蓝四唑(NBT)光还原法测定。(7) Peroxidase (POD) and superoxide dismutase (SOD) activities: measured by ultraviolet absorption method and nitrogen blue tetrazolium (NBT) photoreduction method, respectively.
每个指标至少设3个重复,实验数据以表示,采用Excel和SAS9.0软件进行数据处理和差异显著性分析。At least 3 repetitions were set up for each indicator, and the experimental data were Said that Excel and SAS9.0 software were used for data processing and significance analysis of differences.
实施例8研究不同保鲜剂联合热处理对木薯品质的影响Embodiment 8 studies the impact of different preservatives combined heat treatment on cassava quality
(1)对木薯失重率的影响(1) Effect on weight loss rate of cassava
失重率是评价保鲜效果的重要指标之一。木薯鲜薯水分高达65%,在贮藏过程中重量变化主要是由呼吸作用引发的水分散失,导致块根出现明显的萎蔫和起皱现象,影响外观品质。实施例7的4种不同处理方式下的失重率曲线如图2所示,在贮藏期间所有处理失重率均呈现先上升,而后逐渐趋于平衡。对照组木薯贮藏12d后已开始腐烂,其他处理组失重率与对照组相比,均达到差异显著水平(P<0.05)。由图2可知,1.5wt%壳聚糖+0.15wt%抗坏血酸+0.25wt%氯化钙组合(保鲜剂C)较其他处理失重率略低。The weight loss rate is one of the important indicators to evaluate the preservation effect. The water content of fresh cassava is as high as 65%. The weight change during storage is mainly due to the water loss caused by respiration, which leads to obvious wilting and wrinkling of the root tubers, which affects the appearance quality. The weight loss rate curves under the 4 different treatment methods of Example 7 are shown in Figure 2. During the storage period, the weight loss rates of all treatments first increased, and then gradually tended to balance. The cassava in the control group had begun to rot after being stored for 12 days, and the weight loss rates of the other treatment groups were significantly different from those in the control group (P<0.05). It can be seen from Figure 2 that the weight loss rate of the combination of 1.5wt% chitosan+0.15wt% ascorbic acid+0.25wt% calcium chloride (preservative C) is slightly lower than other treatments.
(2)对木薯硬度的影响(2) Effect on cassava hardness
块根硬度反应果蔬的成熟、衰老情况,是影响木薯贮藏品质的另一个重要因素。木薯贮藏过程中由于水分散失与营养成分的变化,导致其硬度降低,块根出现萎蔫、起皱现象。从图3可看出,不同处理方式下,木薯块根硬度在贮藏期间均呈下降趋势。在第12d,保鲜剂A、B、C处理的果实硬度分别比对照组(CK)高0.2,0.17和0.27kg/cm2,且保鲜剂C处理的块根硬度明显优于其他组,一直处于最高水平。说明壳聚糖、抗坏血酸和氯化钙组合可更有效抑制木薯块根的呼吸作用及后熟软化,使其保持良好的鲜食品质。Root hardness reflects the maturity and senescence of fruits and vegetables, and is another important factor affecting the storage quality of cassava. During cassava storage, due to water loss and changes in nutrient components, its hardness is reduced, and the roots of cassava appear wilting and wrinkling. It can be seen from Figure 3 that under different treatments, the hardness of cassava tubers showed a downward trend during storage. On the 12th day, the firmness of fruits treated with preservatives A, B, and C was 0.2, 0.17, and 0.27kg/cm 2 higher than that of the control group (CK), respectively, and the firmness of root tubers treated with preservative C was significantly better than that of other groups, and was always at the highest Level. It shows that the combination of chitosan, ascorbic acid and calcium chloride can more effectively inhibit the respiration and post-ripening softening of cassava tubers, so that it can maintain good fresh food quality.
(3)对木薯色泽的影响(3) Effect on the color of cassava
由图4可见,在整个贮藏期间,不同处理方式下,木薯果肉色泽L*值呈逐渐下降趋势,说明随着贮藏时间的延长,木薯块根的亮度会逐渐下降。在贮藏第9d,4组处理L*值的下降率分别为:16.4%,18.6%,10.1%,22.6%,下降速率最小的为C组,且差异性极显著(P<0.01)。在12-15d,对照组处理的木薯块根已经开始腐烂,处理方式B、C间差异不显著。整个贮藏期内保鲜剂处理的木薯块根L*值都显著高于对照组,表明保鲜剂处理有利于保持木薯果肉的淡黄色色泽。It can be seen from Figure 4 that during the entire storage period, under different treatments, the color L * value of cassava pulp gradually decreased, indicating that with the prolongation of storage time, the brightness of cassava tubers will gradually decrease. On the 9th day of storage, the decreasing rates of L * values in the four groups were 16.4%, 18.6%, 10.1%, and 22.6%, respectively, and the decreasing rate was the smallest in group C, and the difference was extremely significant (P<0.01). At 12-15 days, the cassava tubers treated by the control group had begun to rot, and there was no significant difference between treatments B and C. The L * value of cassava tubers treated with preservatives was significantly higher than that of the control group during the whole storage period, indicating that the treatment of preservatives is beneficial to maintain the light yellow color of cassava pulp.
(4)木薯淀粉含量的影响(4) The influence of cassava starch content
淀粉含量是木薯的主要营养指标之一。由图5可知,不同处理方式下,木薯块根淀粉含量在贮藏期间的变化总体呈平缓下降趋势,贮藏时间越长,淀粉含量越低。对照组淀粉含量下降最快,保鲜剂C处理的下降最慢。在第3-9d,保鲜剂B处理的块根淀粉含量最高,在第9-15d,经保鲜剂处理的木薯块根淀粉含量明显高于对照组,其木薯淀粉含量始终维持在较高水平,而对照组处理的木薯块根已经开始腐烂,差异性极显著(P<0.01),说明保鲜剂处理可以保持木薯的淀粉含量,防止淀粉的转化。Starch content is one of the main nutritional indicators of cassava. It can be seen from Figure 5 that under different treatment methods, the starch content of cassava tubers during storage generally showed a gentle downward trend, and the longer the storage time, the lower the starch content. The starch content in the control group decreased the fastest, and the preservative C treatment decreased the slowest. On the 3rd to 9th day, the root starch content of preservative B was the highest. On the 9th to 15th day, the starch content of cassava root treated with preservative was significantly higher than that of the control group, and its cassava starch content was always maintained at a high level. The cassava tubers treated in the group had begun to rot, and the difference was extremely significant (P<0.01), indicating that the preservative treatment could maintain the starch content of cassava and prevent starch conversion.
(5)对木薯可溶性固形物(TSS)含量的影响(5) Effect on Cassava Soluble Solids (TSS) Content
TSS含量的高低,在一定程度上反映了果蔬的营养品质。如图6所示,木薯块根的TSS含量在贮藏期内总体呈上升趋势,与对照相比,本发明的保鲜剂处理组的TSS上升的速率相对缓慢,对照组木薯到第12d时TSS含量达到最大值5.29%,随后开始腐烂。3种保鲜剂(A、B、C)处理均可显著降低木薯可溶性固形物含量变化,在第15d,TSS含量分别为3.27%,2.77%,2.55%,没有腐烂。说明保鲜剂处理有利于保持木薯品质,可抑制木薯淀粉向可溶性糖的转化。The level of TSS content reflects the nutritional quality of fruits and vegetables to a certain extent. As shown in Figure 6, the TSS content of cassava tuber root is on the rise in the storage period overall, compares with contrast, the rate that the TSS rise of the preservative agent of the present invention handles group is relatively slow, and the TSS content of control group cassava reaches to 12d when the 12th day. 5.29% max, then starts to rot. Three kinds of preservatives (A, B, C) can significantly reduce the change of cassava soluble solids content. On the 15th day, the TSS content was 3.27%, 2.77%, 2.55%, respectively, and there was no rot. It shows that the preservative treatment is beneficial to maintain the quality of cassava, and can inhibit the transformation of cassava starch into soluble sugar.
实施例9研究不同保鲜剂联合热处理对木薯SOD和POD酶活性的影响Embodiment 9 studies the influence of different preservatives combined heat treatment on cassava SOD and POD enzyme activity
超氧化物歧化酶(SOD)和过氧化物酶(POD)是植物体保护酶系统中重要的酶类。SOD可清除代谢过程中产生的活性氧,对于维持氧代谢平衡起到关键作用。由图7可以看出,随着贮藏时间的延长,各处理木薯块根SOD酶活性均呈先上升后下降趋势。对照组SOD活性高峰出现在第6d,峰值为10.20U/g·FW·h,随后急剧下降,12d后木薯块根腐烂。保鲜剂A、B、C处理的SOD活性高峰均出现在第12d,峰值分别为10.22,11.07和12.16U/g·FW·h,分别比无菌水对照组高17.47%,27.24%和36.32%,处理间差异极显著(P<0.01)。Superoxide dismutase (SOD) and peroxidase (POD) are important enzymes in plant protective enzyme system. SOD can remove reactive oxygen species produced in the metabolic process and play a key role in maintaining the balance of oxygen metabolism. It can be seen from Figure 7 that with the prolongation of storage time, the SOD enzyme activity of cassava tubers in each treatment showed a trend of first increasing and then decreasing. The peak of SOD activity in the control group appeared on the 6th day, and the peak value was 10.20 U/g·FW·h, then dropped sharply, and cassava roots rotted after 12 days. The peaks of SOD activity of preservatives A, B, and C all appeared on the 12th day, and the peaks were 10.22, 11.07, and 12.16 U/g·FW·h, respectively, which were 17.47%, 27.24%, and 36.32% higher than those of the sterile water control group , the difference among treatments was extremely significant (P<0.01).
POD是引起果蔬褐变的关键酶类,可催化过氧化物对酚类物质的氧化,最终导致果蔬组织的褐变,影响果蔬的品质。如图8所示,在贮藏期,四种处理木薯块根POD活性普遍呈先升高再降低的趋势。对照组在第12d达到最高值,随后木薯开始腐烂。在整个贮藏期,对照组木薯POD活性显著高于保鲜剂处理组,处理间差异显著(p<0.05)。在贮藏前期(0~6d),保鲜剂B对POD酶活性的抑制效果优于保鲜剂C,而贮藏后期(9~15d)保鲜剂C对POD酶活性的抑制效果则优于保鲜剂B。由上述结果可知,保鲜处理有利于抑制木薯块根的SOD高活性,有效的维持较低的POD活性,从而延缓贮藏过程中木薯品质劣变和组织衰老进程。POD is the key enzyme that causes the browning of fruits and vegetables. It can catalyze the oxidation of phenolic substances by peroxides, eventually leading to the browning of fruit and vegetable tissues and affecting the quality of fruits and vegetables. As shown in Figure 8, during the storage period, the POD activities of cassava tubers under the four treatments generally showed a trend of first increasing and then decreasing. The control group reached the highest value on the 12th day, and then the cassava began to rot. During the entire storage period, the cassava POD activity in the control group was significantly higher than that in the preservative treatment group, and the difference between treatments was significant (p<0.05). In the early storage period (0-6d), the inhibitory effect of preservative B on POD enzyme activity was better than that of preservative C, while in the late storage period (9-15d), the inhibitory effect of preservative C on POD enzyme activity was better than that of preservative B. From the above results, it can be seen that fresh-keeping treatment is beneficial to inhibit the high activity of SOD in cassava root and effectively maintain the lower POD activity, thereby delaying the quality deterioration and tissue aging process of cassava during storage.
实施例10研究不同保鲜剂联合热处理对木薯总酚的影响Embodiment 10 studies the impact of different preservatives combined heat treatment on cassava total phenols
酚类物质是植物体内重要的次生代谢产物,具有天然的抗氧化活性,对果蔬的品质、色泽、风味和抗逆性有一定的影响。其含量多少直接影响果蔬褐变进程,因此,研究贮藏期间中酚类物质含量的变化规律对于调控木薯块根的品质具有重要意义。不同保鲜剂处理后木薯的多酚含量变化如图9所示,结果表明,贮藏期间,木薯块根总酚含量前期呈上升趋势,然后开始下降,与甘薯、芋艿、莲藕的研究结果相一致。贮藏第9天,各组的多酚含量分别达最大值14.6,13.2,10.1,18.9μg/gFW,三种保鲜剂均有效的抑制了总酚含量的增加,其中保鲜剂C处理抑制效果更好。Phenolic substances are important secondary metabolites in plants, which have natural antioxidant activity and have certain effects on the quality, color, flavor and stress resistance of fruits and vegetables. The amount of its content directly affects the browning process of fruits and vegetables. Therefore, it is of great significance to study the change law of the content of phenolic substances in the storage period for regulating the quality of cassava roots. The changes in polyphenol content of cassava treated with different preservatives are shown in Figure 9. The results showed that during storage, the total phenol content of cassava root showed an upward trend in the early stage, and then began to decline, which was consistent with the research results of sweet potato, taro, and lotus root. On the 9th day of storage, the polyphenol content of each group reached the maximum value of 14.6, 13.2, 10.1, and 18.9 μg/gFW respectively. The three preservatives all effectively inhibited the increase of the total phenol content, and the antistaling agent C treatment had the better inhibitory effect .
木薯采后生理劣变时,主要是第一阶段的生理褐变和第二阶段由微生物引起的软腐。本发明发现,采用复合保鲜剂结合热处理可有效改善木薯的贮藏保鲜效果。本发明中,先用热处理鲜薯块根,减缓呼吸作用、抑制细胞的新陈代谢,而后利用复合保鲜剂浸泡木薯块根,最后采用真空包装室温贮藏,阻止O2的渗入以及水分与CO2的渗出,抑制木薯的褐变作用及表面微生物的生长繁殖,使鲜薯块根的生理生化指标同对照组相比有明显的改善,并且有效降低鲜薯的失水率和营养物质消耗,保持了块根硬度和脆度,延缓淀粉的降解,显著控制了可溶性固形物含量的增加。维持了较高水平的SOD酶活性,延缓过POD酶活性的增加,降低了总酚含量,有效的延长了采后木薯块根的贮藏期,抑制了其品质的劣变,为采后木薯的贮藏保鲜提供了最优的方案。The postharvest physiological deterioration of cassava is mainly physiological browning in the first stage and soft rot caused by microorganisms in the second stage. The present invention finds that the storage and fresh-keeping effect of cassava can be effectively improved by using a composite preservative combined with heat treatment. In the present invention, the fresh potato tubers are first heat-treated to slow down respiration and inhibit cell metabolism, then soak the cassava tubers with a composite preservative, and finally store them in vacuum packaging at room temperature to prevent the infiltration of O and the oozing of moisture and CO . Inhibit the browning of cassava and the growth and reproduction of microorganisms on the surface, so that the physiological and biochemical indicators of fresh potato roots are significantly improved compared with the control group, and effectively reduce the water loss rate and nutrient consumption of fresh potatoes, and maintain the hardness and Crispness, delaying starch degradation, and significantly controlling the increase in soluble solids content. It maintains a high level of SOD enzyme activity, delays the increase of POD enzyme activity, reduces the total phenol content, effectively prolongs the storage period of cassava roots after harvest, and inhibits the deterioration of its quality. Preservation provides the best solution.
本发明研究了不同的保鲜剂结合热处理对木薯块根常温贮藏过程中品质和贮藏期变化的影响,并对贮藏过程中鲜薯生理及品质的动态变化过程进行了监控,寻求了一种全新的适合木薯鲜薯的贮藏保鲜方法,为木薯的采后贮藏保鲜提供理论依据和新的方法。The present invention studies the effects of different preservatives combined with heat treatment on the quality and storage period of cassava tubers during normal temperature storage, and monitors the dynamic change process of fresh potato physiology and quality during storage, and seeks a new suitable method. The storage and preservation method of fresh cassava provides a theoretical basis and a new method for postharvest storage and preservation of cassava.
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