CN105572207A - Convenient capillary three-dimensional sample injection device and use method - Google Patents

Abstract

The invention relates to a convenient capillary three-dimensional sample injection device and a use method. The convenient capillary three-dimensional sample injection device is characterized by comprising a mounting platform and a waste liquid basin fixedly arranged on the mounting platform, wherein the waste liquid basin is connected with a high-voltage wire; an outlet end of a capillary is inserted into the waste liquid basin; a three-dimensional moving device is arranged on the inlet end side of the capillary; a hollow column sample basin and a hollow column buffering liquid basin are arranged on the three-dimensional moving device, so that the inlet end of the capillary can be inserted into the hollow column sample basin and the hollow column buffering liquid basin in sequence along with movement of the three-dimensional moving device. Compared with the conventional manual sample injection and separation moving capillary, the convenient capillary three-dimensional sample injection device has the advantages of more convenience in operation and high accuracy in positioning; furthermore, the risk of electric shock as the hand of an experimenter gets too close to the high-voltage wire is avoided.

Description

Simple capillary three-dimensional sampling device and its application method

Technical field:

The invention relates to a simple capillary three-dimensional sampling device and a using method.

Background technique:

Capillary electrophoresis is an important separation method, which relies on a long and thin tube to separate samples. When the capillary separates samples, the inlet end of the capillary should first be immersed in the sample pool for sample injection, and then pulled out and put into the buffer pool, so that the buffer pushes the sample for separation. That is to say, during the capillary injection separation, the inlet end has to go through the steps of immersing into two different pools respectively.

In the previous separation steps of capillary injection, the inlet ends of the capillary were mainly put into the sample pool and the buffer pool respectively by manual method. The disadvantage of this method is that manual operation is required to align the inlet of the pool each time, and the operator's hand is very close to the high-voltage platinum wire used for driving capillary electrophoresis, so there is often the risk of being shocked by electric shock. Commercialized capillary sampling usually adopts the method of switching the turntable, so that the inlet end of the capillary can be automatically switched between the two pools mechanically. The disadvantage of this method is that it requires the instrument to be equipped with expensive switching devices, and this method is only suitable for the process of capillary electric sampling and pressure sampling. If the capillary adopts siphon sampling, it is difficult to adjust the injection volume of siphon sampling in this commercialized instrument.

Invention content:

The object of the present invention is to provide a simple three-dimensional capillary sampling device, which not only has a simple structure, a reasonable design, and accurate positioning, but also avoids the danger of electric shocks for experimenters.

The simple three-dimensional capillary sampling device of the present invention is characterized in that it includes an installation platform and a waste liquid pool fixed on the installation platform, the negative electrode of the high-voltage electric wire is connected in the waste liquid pool, and the outlet of the capillary is inserted in the waste liquid pool end, the inlet side of the capillary is provided with a three-dimensional moving device, and the three-dimensional moving device is provided with an empty core column sample pool and an empty core column buffer pool, so that the movement of the three-dimensional moving device makes the inlet end of the capillary inserted in sequence Empty Column Sample Cell and Empty Column Buffer Cell.

Further, the above-mentioned three-dimensional moving device includes an upper and a lower elevating frame and a longitudinal slider and a slider guide rail arranged on the elevating frame. Pool casing.

The method for using the simple capillary three-dimensional sampling device of the present invention is characterized in that: the position of the capillary is fixed, the outlet end of the capillary is immersed in the waste liquid pool, the inlet end is suspended, and the upper and lower lifting frames are adjusted to allow the empty core column sample pool and The horizontal position of the empty core column buffer pool is consistent with the capillary inlet end. Push the slider forward to align the empty core column sample pool with the capillary, and clamp it on the outer guide rail with the positioning clip, so that every time the slider is pulled back, as long as The slider will stop when it encounters the positioning clip, and automatically align the inlet end of the capillary with the sample cell of the empty core column; after alignment, push the sample cell of the empty core column to the left to let the inlet end of the capillary penetrate into the sample cell and immerse in the sample solution Complete the sample injection; after the sample injection is completed, pull out the empty core column sample cell to the right; in the same way, continue to push the slider forward so that the empty core column buffer pool is aligned with the capillary, and clamp another positioning clip on the inner side of the guide rail Positioning, so that every time you push forward and touch the positioning clip, the empty core column buffer pool and the inlet end of the capillary are aligned. Push the empty core column buffer pool to the left to let the capillary inlet end penetrate into the buffer pool and immerse in the buffer solution. Complete the separation.

The advantages of the present invention: compared with the previous manual sampling separation and moving capillary, the device of the present invention is more convenient to operate, and the positioning is very accurate, in addition, it also avoids the risk of electric shock caused by the hand of the experimenter getting too close to the high-voltage wire;

Compared with the commercialized automatic turntable sampling device, the device of the present invention can also realize flexible siphon sampling, and the sample injection volume can be changed by changing the length of the capillary inlet end that probes into the pool; the structure and assembly of the device of the present invention It is very convenient, the materials are easy to obtain, and the construction cost is low.

Description of drawings:

Fig. 1 is the structure schematic diagram of simple three-dimensional capillary sampling device of the present invention;

Fig. 2 is a separation diagram of 15 FITC injections using the device of the present invention.

detailed description:

In order to make the above-mentioned features and advantages of the present invention more comprehensible, the following specific embodiments are described in detail with reference to the accompanying drawings.

The simple three-dimensional capillary sampling device of the present invention includes an installation platform 10 and a waste liquid pool 2 fixed on the installation platform. The outlet end of the capillary 3, the inlet end side of the capillary is provided with a three-dimensional moving device 11, the three-dimensional moving device 11 is provided with a hollow core column sample pool 5 and an empty core column buffer pool 6, and the hollow core column sample pool 5 The anode 102 of the high-voltage electric wire is respectively connected to the hollow column buffer pool 6, so that the movement of the three-dimensional moving device makes the inlet end of the capillary sequentially inserted into the hollow column sample pool and the hollow column buffer pool.

Further, the above-mentioned three-dimensional moving device includes upper and lower lifting frames 9 and longitudinal sliders 7 and slider guide rails 8 arranged on the lifting frames. Sleeve 4 for cartridge buffer pool.

The method of using the simple capillary three-dimensional sampling device of the present invention, the position of the capillary is fixed, the outlet end of the capillary is immersed in the waste liquid pool, the inlet end is suspended in the air, and the upper and lower lifting frames are adjusted to allow the empty core column sample pool and the empty core column to buffer The horizontal position of the liquid pool is consistent with the inlet end of the capillary. Push the slider forward to align the hollow column sample pool with the capillary, and clamp it on the outer guide rail with the positioning clip, so that when the slider is pulled back every time, as long as the slider meets The positioning clip can stop and automatically align the inlet end of the capillary with the sample cell of the hollow core column; after alignment, push the sample cell of the empty core column to the left, let the inlet end of the capillary penetrate into the sample cell and immerse in the sample solution to complete the sample injection; After the sample injection is completed, pull out the empty core column sample cell to the right; in the same way, continue to push the slider forward to align the empty core column buffer pool with the capillary, and clamp another positioning clip on the inner side of the guide rail for positioning, so that every Pushing the positioning clip before the second time is the position where the empty core column buffer pool is aligned with the capillary inlet end. Push the empty core column buffer pool to the left to let the capillary inlet end penetrate into the buffer pool and immerse in the buffer solution to complete the separation.

The advantages of the present invention: compared with the previous manual sampling separation and moving capillary, the device of the present invention is more convenient to operate, and the positioning is very accurate, in addition, it also avoids the risk of electric shock caused by the hand of the experimenter getting too close to the high-voltage wire;

Compared with the commercialized automatic turntable sampling device, the device of the present invention can also realize flexible siphon sampling, and the sample injection volume can be changed by changing the length of the capillary inlet end that probes into the pool; the structure and assembly of the device of the present invention It is very convenient, the materials are easy to obtain, and the construction cost is low.

Reproducibility experiment: Using the siphon method, on a simple capillary three-dimensional sampling device The FITC solution with a concentration of 1×10-5mol/L was repeatedly injected and separated 15 times, and the buffer was 10mmol/L borax buffer; the effective separation length of the capillary was 103mm, and the separation voltage was 3000V. The results are shown in Figure 2. The relative average deviation RSDs of peak height and migration time were 3.47% and 0.81%, respectively. It can be seen that the sampling and separation using this device has good reproducibility.

The above-listed preferred embodiments have further described the purpose, technical solutions and advantages of the present invention in detail. It should be understood that the above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Within the spirit and principles of the present invention, any modifications, equivalent replacements, improvements, etc., shall be included within the protection scope of the present invention.

Claims (3)

1. the three-dimensional sampling device of simple type kapillary, it is characterized in that: the waste liquid pool comprising mounting platform and be fixedly located on mounting platform, the negative pole of high-tension bus-bar is connected with in described waste liquid pool, waste liquid pool to be planted the endpiece of kapillary, the import side of described kapillary is provided with three-dimensional moving device, described three-dimensional moving device is provided with hollow column sample cell and hollow column buffer pool, makes the entrance point of kapillary insert hollow column sample cell and hollow column buffer pool successively to allow the movement of three-dimensional moving device.

2. the three-dimensional sampling device of simple type kapillary according to claim 1, it is characterized in that: described three-dimensional moving device comprises upper and lower crane and is located at longitudinal sliding block, the slide block guide rail on crane, and described longitudinal sliding block is provided with the sleeve pipe for sheathed hollow column sample cell and hollow column buffer pool.

3. the using method of the three-dimensional sampling device of simple type kapillary, it is characterized in that: the position of fixed capillary is motionless, the endpiece of kapillary immerses waste liquid pool, entrance point is unsettled, regulate upper and lower crane consistent with capillary inlet end with the horizontal level of hollow column buffer pool by hollow column sample cell, driven forward slide block first allows hollow column sample cell aim at kapillary, be clipped on follower rail with positioning clip, each like this when retracting slide block, just can stop as long as slide block runs into positioning clip, auto-alignment capillary inlet end and hollow column sample cell; After aligning, push away hollow column sample cell left, allow capillary inlet end probe into sample cell and to immerse in sample solution and complete sample introduction; After completing sample introduction, extract hollow column sample cell to the right; In like manner, continue sliding block forward, hollow column buffer pool is allowed to aim at kapillary, another positioning clip location is pressed from both sides in the inboard of guide rail, pushing and shoving positioning clip before each is like this exactly the position that hollow column buffer pool and capillary inlet end are aimed at, and pushes away hollow column buffer pool left and probes into buffer pool by capillary inlet end and immerse in buffer solution and complete separation.