[go: up one dir, main page]

CN105567496B - A kind of thick wine of gold cordyceps sinensis lotus seeds - Google Patents

A kind of thick wine of gold cordyceps sinensis lotus seeds Download PDF

Info

Publication number
CN105567496B
CN105567496B CN201610084153.8A CN201610084153A CN105567496B CN 105567496 B CN105567496 B CN 105567496B CN 201610084153 A CN201610084153 A CN 201610084153A CN 105567496 B CN105567496 B CN 105567496B
Authority
CN
China
Prior art keywords
cordyceps
seed
lotus
weight
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201610084153.8A
Other languages
Chinese (zh)
Other versions
CN105567496A (en
Inventor
汪超
刘璐
卢忠诚
李冬生
石勇
徐宁
胡勇
周梦舟
李玮
高冰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hubei University of Technology
Original Assignee
Hubei University of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hubei University of Technology filed Critical Hubei University of Technology
Priority to CN201610084153.8A priority Critical patent/CN105567496B/en
Publication of CN105567496A publication Critical patent/CN105567496A/en
Application granted granted Critical
Publication of CN105567496B publication Critical patent/CN105567496B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/06Fungi, e.g. yeasts
    • A61K36/062Ascomycota
    • A61K36/066Clavicipitaceae
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/62Nymphaeaceae (Water-lily family)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Botany (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Preparation Of Fruits And Vegetables (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Non-Alcoholic Beverages (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

本发明涉及一种金色虫草莲子稠酒,是用下述方法制备的,剥皮鲜莲子,清洗,常压蒸煮后冷却;放带夹层的塑料池中压实密封保温;将莲子加水磨浆,得莲子浆液,加淀粉酶、纤维素酶和半纤维素酶保温酶解,板框压滤得莲子汁;莲子汁中加葡萄糖、酵母膏等营养素,灭菌,接种虫草菌种子液培养数日得虫草菌丝体发酵液;加白砂糖调糖度,加乳酸菌种子液,高活干酵母,厌氧发酵后,过滤、澄清、加入魔芋低聚糖调配,过滤菌膜杀菌,得虫草莲子稠酒。本发明配方合理,颜色金黄、口感黏稠滑润,酯香浓郁,有提高人体免疫能力等功效。稠酒中虫草多糖含量>0.4g/100mL,虫草素含量>0.05g/100mL,粘度>200mPa·s。The present invention relates to a golden cordyceps lotus seed thick wine, which is prepared by the following method: fresh lotus seeds are peeled, cleaned, cooked at normal pressure and then cooled; put in a plastic pool with an interlayer for compaction, sealing and heat preservation; add water to grind the lotus seeds to obtain Add amylase, cellulase and hemicellulase to the lotus seed slurry, keep warm and enzymatically hydrolyze, plate and frame press filter to obtain lotus seed juice; add glucose, yeast extract and other nutrients to the lotus seed juice, sterilize, inoculate Cordyceps seed solution and cultivate for several days to obtain Cordyceps mycelium fermentation broth; add white sugar to adjust the sugar content, add lactic acid bacteria seed liquid, high-activity dry yeast, after anaerobic fermentation, filter, clarify, add konjac oligosaccharides to blend, filter bacteria membrane to sterilize, and get Cordyceps lotus seed thick wine. The invention has reasonable formula, golden color, sticky and smooth taste, strong ester fragrance, and has the effects of improving human immunity and the like. Cordyceps polysaccharide content > 0.4g/100mL, cordycepin content > 0.05g/100mL, viscosity > 200mPa·s in thick wine.

Description

一种金色虫草莲子稠酒A golden cordyceps lotus seed thick wine

技术领域technical field

本发明属于食品加工酿造技术领域,具体涉及一种金色虫草莲子稠酒。The invention belongs to the technical field of food processing and brewing, and in particular relates to a golden cordyceps lotus seed thick wine.

背景技术Background technique

蛹虫草又名北冬虫夏草,是子囊菌亚门,麦角菌目,麦角菌科,虫草属的一个模式菌种。蛹虫草富含虫草素、虫草酸、虫草多糖、超氧化物歧化酶(SOD)、腺苷、麦角甾醇等丰富的营养物质和功能活性物质,能起到抗疲劳、缓解衰老、提高人体免疫能力、抗癌、预防肿瘤等作用。它与一般的营养强化剂不同的地方在于它是从整体上调节人体机能的平衡、调动机体内部活力、调节人体新陈代谢机能、提高人体免疫力、促使全部的内脏器官机能正常化。虫草菌丝液体发酵产生的营养成分,与子实体类似,但产量更高,生产周期短,过程易控制。Cordyceps militaris, also known as Cordyceps militaris, is a model species of Ascomycota subphylum, Ergotomycetes, Ergotaceae, and Cordyceps genus. Cordyceps militaris is rich in nutrients and functional active substances such as cordycepin, cordycepic acid, cordyceps polysaccharide, superoxide dismutase (SOD), adenosine, ergosterol, etc., which can resist fatigue, relieve aging, and improve human immunity , anti-cancer, tumor prevention and so on. It is different from general nutritional supplements in that it regulates the balance of human body functions as a whole, mobilizes the internal vitality of the body, regulates human metabolism, improves human immunity, and promotes the normalization of all internal organ functions. The nutrient components produced by the liquid fermentation of Cordyceps mycelium are similar to the fruiting body, but the yield is higher, the production cycle is short, and the process is easy to control.

莲子为睡莲科水生草本植物莲的种子。莲子性平、味甘涩,入心、脾、肾经;具补脾止泻、益肾涩清、养心安神之功效。现代药物学研究证实,莲子中的蛋白质、钙、铁、磷和钾含量非常丰富,还含有其他多种维生素、微量元素、荷叶碱、金丝草甙等物质,除可以构成骨骼和牙齿的成分外,还有促进凝血,使某些酶活化,维持神经传导性,镇静神经,维持肌肉的伸缩性和心跳的节律等作用。丰富的磷是细胞核蛋白的主要组成部分,帮助机体进行蛋白质、脂肪、糖类代谢,并维持酸碱平衡。莲子芯所含生物碱具有显著的强心作用,莲芯碱则有较强抗钙及抗心律不齐的作用。Lotus seed is the seed of lotus, an aquatic herbaceous plant of Nymphaeaceae. Lotus seeds are flat in nature, sweet and astringent in taste, and enter the heart, spleen, and kidney meridians; Modern pharmacological research has confirmed that lotus seeds are very rich in protein, calcium, iron, phosphorus and potassium, and also contain other vitamins, trace elements, nuciferine, hypericin and other substances. In addition to the ingredients, it also has the functions of promoting blood coagulation, activating certain enzymes, maintaining nerve conduction, calming nerves, maintaining muscle elasticity and heartbeat rhythm. Rich phosphorus is the main component of nuclear protein, which helps the body to metabolize protein, fat and sugar, and maintain acid-base balance. The alkaloids contained in the lotus seed core have a significant cardiotonic effect, and the lotus core has a strong anti-calcium and anti-arrhythmia effect.

魔芋低聚糖是魔芋多糖的水解产物,味微甜,清爽纯正,有魔芋特有清香,是一种可溶性膳食纤维,具有不被人体胃肠道消化,但可以促进双歧杆菌增值并抑制有害菌群生长,调节肠道菌群,润肠通便,改善人体肠道功能。Konjac oligosaccharides are hydrolyzed products of konjac polysaccharides, which taste slightly sweet, refreshing and pure, and have the unique fragrance of konjac. Group growth, regulate intestinal flora, laxative, improve human intestinal function.

现有关于虫草酒的专利多是用白酒对虫草进行浸泡或将虫草提取液与白酒调配而成的,对于虫草和虫草中有效功能活性物质的利用有限。莲子加工的产品主要有干莲子、莲蓉、莲子汁和莲子酒。现有的莲子酒生产主要以纯莲子为主,干莲子通过白酒浸泡制得的配制酒,或莲子通过蒸煮、拌曲等工序,进行固态发酵,固态蒸馏制得的莲子白酒。如CN101831371公开了一种莲子白酒的生产工艺,该工艺将莲子粉碎、蒸煮、冷散、拌曲、入缸大渣发酵、酒醅出缸、蒸馏、勾兑得成品酒;CN 103571682公开了一种莲子酒的生产工艺,该工艺将莲子打浆、蒸煮、发酵、压榨、过滤得成品酒;CN 102181343公开了一种莲子酒的生产工艺,该莲子酒经制曲、莲子蒸煮、粉碎、加入酒母发酵,蒸馏、陈酿、调配、过滤处理,即得莲子酒;Most of the existing patents on Cordyceps wine are made by soaking Cordyceps with white wine or blending Cordyceps extract with white wine, and the utilization of effective functional active substances in Cordyceps and Cordyceps is limited. The processed products of lotus seeds mainly include dried lotus seeds, lotus seed paste, lotus seed juice and lotus seed wine. Existing lotus seed wine production is mainly based on pure lotus seeds, dried lotus seeds are prepared by soaking in white wine, or lotus seeds are fermented in solid state and distilled in solid state through processes such as cooking and mixing koji. For example, CN101831371 discloses a production process of lotus seed liquor. In this process, lotus seeds are crushed, boiled, cooled, mixed with koji, fermented with large slag in a tank, fermented with fermented grains, distilled, and blended to obtain a finished wine; CN 103571682 discloses a A production process of lotus seed wine, which involves beating lotus seeds, steaming, fermenting, squeezing, and filtering to obtain finished wine; CN 102181343 discloses a production process of lotus seed wine, which involves making koji, cooking lotus seeds, crushing, and adding distiller's mother to ferment , distilled, aged, blended, and filtered to obtain lotus seed wine;

发明内容Contents of the invention

本发明的目的是针对上述现状,旨在提供一种饮用方便,色泽黑亮,口感黏稠爽润、酯香浓郁,能降低血糖、缓解疲劳、提高人体免疫能力、清热解火、补血滋阴、生津止渴、调节肠道菌群、润肠通便;工艺简单,方法合理,操作简便,生产设备成本低、自动化程度高、可操作性好、不易染菌,劳动强度低,生产效益高的金色虫草莲子稠酒。The purpose of the present invention is to aim at the above-mentioned status quo, aiming to provide a kind of drinkable, black and bright color, thick and refreshing mouthfeel, rich ester aroma, can lower blood sugar, relieve fatigue, improve human immunity, clear away heat and relieve fire, nourish blood and nourish yin, Quenching thirst, regulating intestinal flora, laxative; simple process, reasonable method, easy operation, low cost of production equipment, high degree of automation, good operability, not easy to infect bacteria, low labor intensity, high production efficiency Golden Cordyceps and lotus seed thick wine.

本发明目的的实现方式为,一种金色虫草莲子稠酒,是由下述方法制备的,制备的具体步骤为:The way to achieve the purpose of the present invention is that a kind of golden cordyceps lotus seed thick wine is prepared by the following method, and the specific steps of preparation are:

1)将剥皮鲜莲子,洗净,常压蒸煮10-20min后,冷却至54-56℃;1) Wash the peeled fresh lotus seeds, cook at normal pressure for 10-20 minutes, and cool to 54-56°C;

2)将步骤1)的莲子放入池中,压实、密封,40-55℃保温48-72h;2) Put the lotus seeds of step 1) into the pond, compact and seal, and keep warm at 40-55°C for 48-72h;

3)将经步骤2)保温的莲子按重量份数为220-240的莲子与水1:1-1.5的比例加水磨浆,得莲子浆液,浆液中加入重量份数为0.024-0.031淀粉酶、0.031-0.037纤维素酶和0.019-0.027半纤维素酶,45-55℃保温酶解5-7h,板框压滤取汁,得莲子汁;3) adding water to the lotus seeds that have been insulated in step 2) according to the ratio of 220-240 parts by weight to lotus seeds and water 1:1-1.5, to obtain lotus seed slurry, adding 0.024-0.031 parts by weight of amylase, 0.031-0.037 cellulase and 0.019-0.027 hemicellulase, 45-55 ℃ heat preservation enzymolysis for 5-7h, plate and frame press filter to extract juice, to obtain lotus seed juice;

4)向步骤3)所得莲子汁中加入其质量1%葡萄糖、0.3%KH2PO4、0.15%MgSO4·7H2O、0.01%维生素B1、0.2%酵母膏,4%豆粕,灭菌,接种重量份数为50-60的虫草菌种子液,23℃培养7d,得虫草菌丝体发酵液;4) Add its quality 1% glucose, 0.3% KH2PO4, 0.15% MgSO4.7H2O, 0.01% vitamin B1, 0.2% yeast extract, 4% soybean meal to the lotus seed juice obtained in step 3), sterilize, and inoculate 50 parts by weight Cordyceps seed liquid at -60°C, cultured at 23°C for 7 days, to obtain Cordyceps mycelium fermentation liquid;

5)将步骤4)所得虫草菌丝体发酵液直接加入重量份数为80-100的白砂糖调整糖度;或将步骤4)所得虫草菌丝体发酵液经降膜蒸发器浓缩至原来体积的1/2加入重量份数为80-100的白砂糖调整糖度;5) directly add white granulated sugar of 80-100 parts by weight to the fermented liquid of Cordyceps mycelium obtained in step 4) to adjust the sugar content; or concentrate the fermented liquid of Cordyceps mycelium obtained in step 4) to the original volume by falling film 1/2 is added the white granulated sugar that is 80-100 parts by weight to adjust the sugar content;

调整甜度后18-25℃保温2h,加入重量份数为12.0-15.0的乳酸菌种子液,厌氧发酵36-48h后,再加入活化好的,重量份数为1.1-1.5的高活干酵母,10-15℃厌氧发酵15-20d后,过滤、澄清、加入重量份数为10-16的魔芋低聚糖调配、灌装、过滤膜除菌,得金色虫草莲子稠酒。After adjusting the sweetness, keep warm at 18-25°C for 2 hours, add 12.0-15.0 parts by weight of lactic acid bacteria seed liquid, and after anaerobic fermentation for 36-48 hours, then add activated high-activity dry yeast with 1.1-1.5 parts by weight After anaerobic fermentation at 10-15° C. for 15-20 days, filter, clarify, add konjac oligosaccharides in parts by weight of 10-16 to prepare, fill, and filter membrane to sterilize to obtain golden cordyceps lotus seed thick wine.

金色虫草莲子稠酒中虫草多糖含量>0.4g/100mL,虫草素含量>0.05g/100mL,粘度>200mPa·s。Cordyceps polysaccharide content>0.4g/100mL, cordycepin content>0.05g/100mL, viscosity>200mPa·s in golden cordyceps lotus seed thick wine.

与现有技术相比,本发明有如下优点和显著的进步:Compared with prior art, the present invention has following advantage and remarkable progress:

1、对莲子进行常压蒸煮以达到灭菌灭酶的目的,一方面可以防止莲子变质,一方面通过灭酶活以保障下一步在塑料池中,将莲子压实、密封、40-55℃保温48-72h的过程促进稠液的形成;1. The lotus seeds are steamed at atmospheric pressure to achieve the purpose of sterilization and enzyme elimination. On the one hand, it can prevent the lotus seeds from deteriorating. On the other hand, it can ensure the next step by inactivating the enzyme activity in the plastic pool. The lotus seeds are compacted, sealed, and kept at 40-55°C The process of keeping warm for 48-72 hours promotes the formation of thick liquid;

2、莲子浆液中加淀粉酶、纤维素酶和半纤维素酶酶解莲藕汁,使莲子汁更利于虫草菌生长;2. Add amylase, cellulase and hemicellulase to the lotus seed slurry to enzymolyze the lotus root juice, so that the lotus seed juice is more conducive to the growth of Cordyceps fungus;

3、以莲子汁为虫草菌丝体培养基,充分利用了其中丰富的碳水化合物,充分利用了其中丰富的碳水化合物,所选虫草菌能在莲子汁做培养基的环境下良好生长并大量产菌丝;3. Taking lotus seed juice as the culture medium for Cordyceps mycelium, making full use of the rich carbohydrates therein, the selected Cordyceps fungus can grow well in the environment of lotus seed juice as the culture medium and produce a large number of hyphae;

4、成品酒中富含虫草多糖、魔芋低聚糖等可溶性多糖和莲子粘液类物质,使酒更加粘稠4. The finished wine is rich in soluble polysaccharides such as Cordyceps polysaccharides, konjac oligosaccharides and lotus seed mucus substances, making the wine more viscous

5、成品酒中含有的虫草多糖、虫草素和莲子粘液类物质中的糖蛋白和可溶性膳食纤维——魔芋低聚糖等功能活性成分,既有效保留了莲子原来风味,又使成品酒为透亮的金黄色,并具有抗疲劳、缓解衰老、提高人体免疫能力、抗癌、预防肿瘤,补益脾胃,益血生肌、调节肠道菌群等保健功能;5. Functional active ingredients such as Cordyceps polysaccharide, cordycepin, glycoprotein in lotus seed mucus and soluble dietary fiber - konjac oligosaccharides contained in the finished wine not only effectively retain the original flavor of lotus seeds, but also make the finished wine translucent It is golden yellow, and has health functions such as anti-fatigue, anti-aging, improving human immunity, anti-cancer, preventing tumors, nourishing the spleen and stomach, nourishing blood and strengthening muscles, and regulating intestinal flora;

6、工艺简单,方法合理,操作简便,生产设备成本低、自动化程度高、可操作性好、不易染菌,劳动强度低,生产效益高。6. The process is simple, the method is reasonable, the operation is simple, the cost of production equipment is low, the degree of automation is high, the operability is good, it is not easy to infect bacteria, the labor intensity is low, and the production efficiency is high.

具体实施方式Detailed ways

本发明是用下述方法制备的,剥皮鲜莲子,清洗,常压蒸煮后冷却;放带夹层的塑料池中压实密封保温;将莲子加水磨浆,得莲子浆液,加淀粉酶、纤维素酶和半纤维素酶保温酶解,板框压滤得莲子汁;莲子汁中加葡萄糖、酵母膏等营养素,灭菌,接种虫草菌种子液培养数日得虫草菌丝体发酵液;虫草菌丝体发酵液加白砂糖调糖度,加乳酸菌种子液,高活干酵母,厌氧发酵后,过滤、澄清、加入魔芋低聚糖调配,过滤菌膜杀菌,得金色虫草莲子稠酒。The present invention is prepared by the following method: peel fresh lotus seeds, wash them, cook them under normal pressure and cool them down; put them in a plastic pool with an interlayer for compaction, sealing and heat preservation; add water to grind lotus seeds to obtain lotus seed slurry, add amylase and cellulose Enzyme and hemicellulase heat preservation enzymolysis, plate and frame pressure filtration to obtain lotus seed juice; add glucose, yeast extract and other nutrients to lotus seed juice, sterilize, inoculate Cordyceps seed liquid and cultivate for several days to obtain Cordyceps mycelium fermentation liquid; Add white granulated sugar to the silk fermented liquid to adjust the sugar content, add lactic acid bacteria seed liquid, high-activity dry yeast, after anaerobic fermentation, filter, clarify, add konjac oligosaccharides to blend, filter bacteria film to sterilize, and get golden cordyceps lotus seed thick wine.

步骤2)中将步骤1)的莲子放入带夹层的塑料池中。In step 2), the lotus seeds of step 1) are put into the plastic pool with interlayer.

步骤4)中所述的虫草菌为蛹虫草(中国工业微生物菌种保藏管理中心,保藏号CICC14014),虫草种子液按如下方法制备而成:用试管固态培养基活化保藏的蛹虫草菌,28℃培养5d后,取面积为4cm2的菌丝块加入到100mL灭菌后的液体种子培养基中,23℃培养3d,得蛹虫草菌种子液。The Cordyceps militaris described in step 4) is Cordyceps militaris (China Industrial Microbial Strain Preservation Management Center, preservation number CICC14014), and the Cordyceps seed liquid is prepared as follows: the Cordyceps militaris preserved by activating the solid medium in a test tube, 28 After culturing at ℃ for 5 days, the mycelium block with an area of 4 cm was added to 100 mL of sterilized liquid seed culture medium, and cultivated at 23 ℃ for 3 days to obtain the seed liquid of Cordyceps militaris.

所述固态培养基组成为:20%马铃薯汁1.0L,葡萄糖20g,KH2PO4 3g,MgSO4·7H2O1.5g,维生素B1 0.1g,酵母膏2g,琼脂15g。The solid medium consists of: 1.0L of 20% potato juice, 20g of glucose, 3g of KH2PO4, 1.5g of MgSO4.7H2O, 0.1g of vitamin B1, 2g of yeast extract and 15g of agar.

所述液体种子培养基组成为:20%马铃薯汁1.0L,葡萄糖20g,KH2PO4 3g,MgSO4·7H2O 1.5g,萌芽米30g,豆粕40g,维生素B1 0.1g,酵母膏2g。The liquid seed medium consists of: 1.0L of 20% potato juice, 20g of glucose, 3g of KH2PO4, 1.5g of MgSO4.7H2O, 30g of germinated rice, 40g of soybean meal, 0.1g of vitamin B1, and 2g of yeast extract.

步骤5)中所述的乳酸菌种子液按如下方法制备而成:用试管MRS固态培养基活化乳酸菌,30℃培养28h,得活化后的乳酸菌,用接种环刮取菌丝接种到200mL的MRS液态培养基中培养制备一级乳酸菌种子液,28℃培养48h,将一级乳酸菌种子液按10%的接种量接种到2L的MRS液态发酵培养基扩大培养为二级乳酸菌种子液。The lactic acid bacteria seed solution described in step 5) is prepared as follows: activate the lactic acid bacteria with a test tube MRS solid medium, culture them at 30°C for 28 hours to obtain the activated lactic acid bacteria, scrape the hyphae with an inoculation loop and inoculate them into 200 mL of MRS liquid Cultivate and prepare the primary lactic acid bacteria seed solution in the culture medium, cultivate it at 28°C for 48h, inoculate the primary lactic acid bacteria seed solution into 2L of MRS liquid fermentation medium at an inoculum size of 10%, and expand the cultivation into a secondary lactic acid bacteria seed solution.

所述乳酸菌为短乳杆菌(中国工业微生物菌种保藏管理中心,保藏号CICC20014)。The lactic acid bacteria is Lactobacillus brevis (China Industrial Microorganism Culture Collection Management Center, preservation number CICC20014).

步骤5)中所述的高活干酵母(中国工业微生物菌种保藏管理中心,保藏号CICC31121)的活化方法为:按酵母与葡萄糖溶液1:10的质量比,将酵母加入到2%的葡萄糖溶液中,30℃活化15-30min。The activation method of the high-activity dry yeast (China Industrial Microorganism Culture Collection Management Center, preservation number CICC31121) described in step 5) is: according to the mass ratio of yeast and glucose solution 1:10, yeast is added to 2% glucose In the solution, activate at 30°C for 15-30min.

所述的魔芋低聚糖为,魔芋多糖经适当酶解所得分子量在3万~15万道尔顿的魔芋低聚糖。The konjac oligosaccharides are konjac oligosaccharides with a molecular weight of 30,000-150,000 Daltons obtained through proper enzymatic hydrolysis of konjac polysaccharides.

优选的,各原料的重量份数为莲子230-240、虫草菌种子液53-55、白砂糖90-95、淀粉酶0.029-0.031、纤维素酶0.035-0.037、半纤维素酶0.025-0.027、乳酸菌种子液14.0-15.0、高活干酵母1.2-1.4、魔芋低聚糖13-16。Preferably, the parts by weight of each raw material are 230-240 parts by weight of lotus seeds, 53-55 parts of Cordyceps seed liquid, 90-95 parts of white sugar, 0.029-0.031 amylase, 0.035-0.037 cellulase, 0.025-0.027 hemicellulase, Lactic acid bacteria seed liquid 14.0-15.0, high activity dry yeast 1.2-1.4, konjac oligosaccharides 13-16.

更优选之一的,各原料的重量份数为莲子230、虫草菌种子液55、白砂糖95、淀粉酶0.031、纤维素酶0.037、半纤维素酶0.027、乳酸菌种子液15.0、高活干酵母1.4、魔芋低聚糖16。More preferably, the parts by weight of each raw material are 230 parts by weight of lotus seeds, 55 parts of Cordyceps seed liquid, 95 parts of white sugar, 0.031 amylase, 0.037 cellulase, 0.027 hemicellulase, 15.0 parts of lactic acid bacteria seed liquid, and high activity dry yeast 1.4. Konjac oligosaccharides 16.

更优选之一的,各原料的重量份数为莲子235、虫草菌种子液54、白砂糖92、淀粉酶0.030、纤维素酶0.036、半纤维素酶0.026、乳酸菌种子液14.3、高活干酵母1.3、魔芋低聚糖14。More preferably, the parts by weight of each raw material are 235 lotus seeds, 54 cordyceps seed liquid, 92 white sugar, 0.030 amylase, 0.036 cellulase, 0.026 hemicellulase, 14.3 lactic acid bacteria seed liquid, and high-activity dry yeast 1.3. Konjac oligosaccharides14.

下面用具体实施例对本发明作进一步描述。The present invention will be further described below with specific examples.

实施例1:Example 1:

1)将剥皮鲜莲子,洗净,常压蒸煮20min后,冷却至55℃;1) Wash the peeled fresh lotus seeds, cook at normal pressure for 20 minutes, and cool to 55°C;

2)将步骤1)的莲子放入带夹层的塑料池中,压实、密封,55℃保温48h;2) Put the lotus seeds from step 1) into a plastic pool with an interlayer, compact and seal, and keep warm at 55°C for 48h;

3)将经步骤2)保温的莲子按重量份数为220的莲子与水1:1的比例加水磨浆,得莲子浆液,在浆液中加入重量份数为0.024淀粉酶、0.031纤维素酶和0.027半纤维素酶,55℃保温酶解5h,板框压滤取汁,得莲子汁;3) adding water to the lotus seeds that have been insulated in step 2) according to the ratio of 220 parts by weight to lotus seeds and water 1:1, to obtain lotus seed slurry, and adding 0.024 parts by weight of amylase, 0.031 cellulase and 0.027 hemicellulase, enzymatic hydrolysis at 55°C for 5 hours, plate and frame pressure filtration to extract juice to obtain lotus seed juice;

4)向步骤3)所得莲子汁中加入其质量1%葡萄糖、0.3%KH2PO4、0.15%MgSO4·7H2O、0.01%维生素B1、0.2%酵母膏,4%豆粕,灭菌,接种重量份数为60的虫草菌种子液,23℃培养7d,得虫草菌丝体发酵液;4) Add its quality 1% glucose, 0.3% KH2PO4, 0.15% MgSO4.7H2O, 0.01% vitamin B1, 0.2% yeast extract, 4% soybean meal to the lotus seed juice obtained in step 3), sterilize, and the number of inoculated parts by weight is 60 The Cordyceps fungus seed liquid was cultivated at 23°C for 7 days to obtain the Cordyceps mycelium fermentation liquid;

5)将步骤4)所得虫草菌丝体发酵液经降膜蒸发器浓缩至原来体积的1/2,加重量份数为80的白砂糖调整糖度,25℃保温2h,加入重量份数为12.0的乳酸菌种子液,厌氧发酵36h后,再加入活化好的重量份数为1.1的高活干酵母,10℃厌氧发酵20d后,过滤、澄清、加入重量份数为10的魔芋低聚糖调配、灌装、过滤膜除菌,得金色虫草莲子稠酒。5) Concentrate the Cordyceps mycelium fermentation liquid obtained in step 4) to 1/2 of the original volume by a falling film evaporator, add 80 parts by weight of white sugar to adjust the sugar content, keep it warm at 25°C for 2 hours, and add 12.0 parts by weight lactic acid bacteria seed liquid, after anaerobic fermentation for 36h, then add activated high-activity dry yeast with a weight fraction of 1.1, and after anaerobic fermentation at 10°C for 20 days, filter, clarify, and add konjac oligosaccharides with a weight fraction of 10 Blending, filling, and filter membrane sterilization, the golden cordyceps lotus seed thick wine is obtained.

实施例2、同实施例1,不同的是,Embodiment 2, with embodiment 1, the difference is,

1)将剥皮鲜莲子,洗净,常压蒸煮15min后,冷却至56℃;1) Wash the peeled fresh lotus seeds, cook at normal pressure for 15 minutes, and cool to 56°C;

2)将步骤1)的莲子放入带夹层的塑料池中,压实、密封,40℃保温72h;2) Put the lotus seeds from step 1) into a plastic pool with an interlayer, compact and seal, and keep warm at 40°C for 72 hours;

3)将经步骤2)保温的莲子按重量份数为240的莲子与水1:1.3的比例加水磨浆,得莲子浆液,在浆液中加入重量份数为0.031淀粉酶、0.037纤维素酶和0.019半纤维素酶,50℃保温酶解6h,板框压滤取汁,得莲子汁;3) adding water to the lotus seeds incubated in step 2) according to the ratio of 240 parts by weight to lotus seeds and water 1:1.3, to obtain lotus seed slurry, adding 0.031 parts by weight of amylase, 0.037 cellulase and 0.019 hemicellulase, heat-preserved at 50°C for 6 hours, press-filtered to extract the juice, and obtained lotus seed juice;

4)向步骤3)所得莲子汁中加入1%葡萄糖、0.3%KH2PO4、0.15%MgSO4·7H2O、0.01%维生素B1、0.2%酵母膏,4%豆粕,灭菌,接种重量份数为50的虫草菌种子液;4) Add 1% glucose, 0.3% KH2PO4, 0.15% MgSO4 7H2O, 0.01% vitamin B1, 0.2% yeast extract, 4% soybean meal to the lotus seed juice obtained in step 3), sterilize, and inoculate 50 parts by weight of Cordyceps sinensis Bacteria seed liquid;

5)将步骤4)所得虫草菌丝体发酵液直接加重量份数为100的白砂糖调整糖度,20℃保温2h,加入重量份数为15.0的乳酸菌种子液,厌氧发酵48h后,再加入活化好的重量份数为1.5的高活干酵母,15℃厌氧发酵15d后,过滤、澄清、加入重量份数为16的魔芋低聚糖调配、灌装、过滤膜除菌,得金色虫草莲子稠酒。5) Add 100 parts by weight of white granulated sugar directly to the fermented broth of Cordyceps mycelia obtained in step 4) to adjust the sugar content, keep warm at 20°C for 2 hours, add 15.0 parts by weight of lactic acid bacteria seed liquid, and after anaerobic fermentation for 48 hours, add Activated high activity dry yeast with 1.5 parts by weight is anaerobically fermented at 15°C for 15 days, filtered, clarified, prepared by adding konjac oligosaccharides with 16 parts by weight, filled, and sterilized by filter membrane to obtain golden caterpillar fungus Liquor with lotus seeds.

实施例3、同实施例1,不同的是,Embodiment 3, with embodiment 1, the difference is,

1)将剥皮鲜莲子,洗净,常压蒸煮10min后,冷却至54℃;1) Wash the peeled fresh lotus seeds, cook at normal pressure for 10 minutes, and cool to 54°C;

2)将步骤1)的莲子放入带夹层的塑料池中,压实、密封,40℃保温72h;2) Put the lotus seeds from step 1) into a plastic pool with an interlayer, compact and seal, and keep warm at 40°C for 72 hours;

3)将经步骤2)保温的莲子按重量份数为230的莲子与水1:1.5的比例加水磨浆,得莲子浆液,在浆液中加入重量份数为0.031淀粉酶、0.037纤维素酶和0.025半纤维素酶,45℃保温酶解7h,板框压滤取汁,得莲子汁;3) adding water to the lotus seeds incubated in step 2) according to the ratio of 230 parts by weight to lotus seeds and water 1:1.5, to obtain lotus seed slurry, adding 0.031 parts by weight of amylase, 0.037 cellulase and 0.025 hemicellulase, 45°C heat preservation enzymolysis for 7 hours, plate and frame press filtration to get juice, to obtain lotus seed juice;

4)向步骤3)所得莲子汁中加入其质量1%葡萄糖等,灭菌,接种重量份数为55的虫草菌种子液;4) Add its quality 1% glucose etc. to the lotus seed juice gained in step 3), sterilize, and inoculate the Cordyceps seed liquid with 55 parts by weight;

5)将步骤4)所得虫草菌丝体发酵液直接加重量份数为95的白砂糖调整糖度,18℃保温2h,加入重量份数为15.0的乳酸菌种子液,厌氧发酵40h后,再加入活化好的重量份数为1.4的高活干酵母,10℃厌氧发酵18d后,过滤、澄清、加入重量份数为16的魔芋低聚糖调配。5) The Cordyceps mycelium fermentation liquid obtained in step 4) is directly added with 95 parts by weight of white granulated sugar to adjust the sugar content, kept at 18°C for 2 hours, added 15.0 parts by weight of lactic acid bacteria seed liquid, and after anaerobic fermentation for 40 hours, then added 1.4 parts by weight of activated high-activity dry yeast is anaerobically fermented at 10°C for 18 days, filtered, clarified, and 16 parts by weight of konjac oligosaccharides are added for preparation.

实施例4、同实施例1,不同的是,Embodiment 4, with embodiment 1, the difference is,

1)将剥皮鲜莲子,洗净,常压蒸煮10min后,冷却至55℃;1) Wash the peeled fresh lotus seeds, cook at normal pressure for 10 minutes, and cool to 55°C;

2)将步骤1)的莲子放入带夹层的塑料池中,压实、密封,50℃保温50h;2) Put the lotus seeds of step 1) into a plastic pool with an interlayer, compact and seal, and keep warm at 50°C for 50h;

3)将经步骤2)保温的莲子按重量份数为240的莲子与水1:1.3的比例加水磨浆,得莲子浆液,在浆液中加入重量份数为0.029淀粉酶、0.035纤维素酶和0.027半纤维素酶,45℃保温酶解7h,板框压滤取汁,得莲子汁;3) adding water to the lotus seeds that have been insulated in step 2) according to the ratio of 240 parts by weight to lotus seeds and water 1:1.3, to obtain a lotus seed slurry, adding 0.029 parts by weight of amylase, 0.035 parts of cellulase and 0.027 hemicellulase, heat preservation at 45°C for 7 hours, plate and frame press filter to extract juice, to obtain lotus seed juice;

4)向步骤3)所得莲子汁中加入其质量1%葡萄糖等,灭菌,接种重量份数为53的虫草菌种子液;4) Add its quality 1% glucose etc. to the lotus seed juice gained in step 3), sterilize, and inoculate the Cordyceps seed liquid with 53 parts by weight;

5)将步骤4)所得虫草菌丝体发酵液直接加重量份数为90的白砂糖调整糖度,18℃保温2h,加入重量份数为14.0的乳酸菌种子液,厌氧发酵40h后,再加入活化好的重量份数为1.2的高活干酵母,12℃厌氧发酵18d后,过滤、澄清、加入重量份数为13的魔芋低聚糖调配。5) The Cordyceps mycelia fermentation liquid obtained in step 4) is directly added with 90 parts by weight of white granulated sugar to adjust the sugar content, kept at 18°C for 2 hours, added 14.0 parts by weight of lactic acid bacteria seed liquid, and after anaerobic fermentation for 40 hours, then added 1.2 parts by weight of activated high-activity dry yeast is anaerobically fermented at 12°C for 18 days, filtered, clarified, and 13 parts by weight of konjac oligosaccharides are added for preparation.

实施例5、同实施例1,不同的是,Embodiment 5, with embodiment 1, the difference is,

1)将剥皮鲜莲子,洗净,常压蒸煮15min后,冷却至55℃;1) Wash the peeled fresh lotus seeds, cook at normal pressure for 15 minutes, and cool to 55°C;

2)将步骤1)的莲子放入带夹层的塑料池中,压实、密封,50℃保温50h;2) Put the lotus seeds of step 1) into a plastic pool with an interlayer, compact and seal, and keep warm at 50°C for 50h;

3)将经步骤2)保温的莲子按重量份数为230的莲子与水1:1.3的比例加水磨浆,得莲子浆液,在浆液中加入重量份数为0.031淀粉酶、0.037纤维素酶和0.027半纤维素酶,45℃保温酶解7h,板框压滤取汁,得莲子汁;3) adding water to the lotus seeds incubated in step 2) according to the ratio of 230 parts by weight to lotus seeds and water 1:1.3, to obtain lotus seed slurry, adding 0.031 parts by weight of amylase, 0.037 cellulase and 0.027 hemicellulase, heat preservation at 45°C for 7 hours, plate and frame press filter to extract juice, to obtain lotus seed juice;

4)向步骤3)所得莲子汁中加入其质量1%葡萄糖等,灭菌,接种重量份数为55的虫草菌种子液;4) Add its quality 1% glucose etc. to the lotus seed juice gained in step 3), sterilize, and inoculate the Cordyceps seed liquid with 55 parts by weight;

5)将步骤4)所得虫草菌丝体发酵液经降膜蒸发器浓缩至原来体积的1/2,加重量份数为95的白砂糖调整糖度,20℃保温2h,加入重量份数为15.0的乳酸菌种子液,厌氧发酵40h后,再加入活化好的重量份数为1.4的高活干酵母,12℃厌氧发酵18d后,过滤、澄清、加入重量份数为16的魔芋低聚糖调配。5) Concentrate the Cordyceps mycelium fermentation liquid obtained in step 4) to 1/2 of the original volume by a falling film evaporator, add 95 parts by weight of white granulated sugar to adjust the sugar content, keep warm at 20°C for 2 hours, and add 15.0 parts by weight lactic acid bacteria seed liquid, after anaerobic fermentation for 40h, then add activated high-activity dry yeast in parts by weight of 1.4, after anaerobic fermentation at 12°C for 18 days, filter, clarify, and add konjac oligosaccharides in parts by weight of 16 deployment.

金色虫草莲子稠酒为金黄色,酯香浓郁、口感爽滑,酒精度为10.4%(体积分数)、还原糖含量0.46g/100mL、乙酸乙酯含量239.33mg/L、乳酸乙酯含量121.17mg/L、虫草多糖含量0.88g/100mL、虫草素含量0.20g/100mL,莲子粘液类物质含量13.0g/100mL,粘度为287mPa·s。The golden cordyceps lotus seed thick wine is golden yellow, with strong ester aroma and smooth taste, with an alcohol content of 10.4% (volume fraction), reducing sugar content of 0.46g/100mL, ethyl acetate content of 239.33mg/L, and ethyl lactate content of 121.17mg /L, Cordyceps polysaccharide content 0.88g/100mL, cordycepin content 0.20g/100mL, lotus seed mucus content 13.0g/100mL, viscosity 287mPa·s.

实施例6、同实施例1,不同的是,Embodiment 6, with embodiment 1, the difference is,

1)将剥皮鲜莲子,洗净,常压蒸煮20min后,冷却至54℃;1) Wash the peeled fresh lotus seeds, cook them under normal pressure for 20 minutes, and cool to 54°C;

2)将步骤1)的莲子放入带夹层的塑料池中,压实、密封,40℃保温72h;2) Put the lotus seeds from step 1) into a plastic pool with an interlayer, compact and seal, and keep warm at 40°C for 72 hours;

3)将经步骤2)保温的莲子按重量份数为235的莲子与水1:1.5的比例加水磨浆,得莲子浆液,在浆液中加入重量份数为0.030淀粉酶、0.036纤维素酶和0.026半纤维素酶,45℃保温酶解7h,板框压滤取汁,得莲子汁;3) adding water to the lotus seeds incubated in step 2) according to the ratio of 235 parts by weight to lotus seeds and water 1:1.5, to obtain lotus seed slurry, adding 0.030 parts by weight of amylase, 0.036 cellulase and 0.026 hemicellulase, heat preservation at 45°C for 7 hours, plate and frame press filter to extract juice, to obtain lotus seed juice;

4)向步骤3)所得莲子汁中加入其质量1%葡萄糖等,灭菌,接种重量份数为54的虫草菌种子液;4) Add its quality 1% glucose etc. to the lotus seed juice obtained in step 3), sterilize, and inoculate the Cordyceps seed liquid with 54 parts by weight;

5)将步骤4)所得虫草菌丝体发酵液直接加重量份数为92的白砂糖调整糖度,18℃保温2h,加入重量份数为14.3的乳酸菌种子液,厌氧发酵40h后,再加入活化好的重量份数为1.3的高活干酵母,12℃厌氧发酵20d后,过滤、澄清、加入重量份数为14的魔芋低聚糖调配。5) The Cordyceps mycelium fermentation liquid obtained in step 4) is directly added with 92 parts by weight of white granulated sugar to adjust the sugar content, kept at 18°C for 2 hours, added 14.3 parts by weight of lactic acid bacteria seed liquid, and after anaerobic fermentation for 40 hours, then added 1.3 parts by weight of activated high-activity dry yeast is anaerobically fermented at 12°C for 20 days, filtered, clarified, and 14 parts by weight of konjac oligosaccharides are added for preparation.

采取上述操作制备的金色虫草莲子稠酒,相比实施例1,酯香更浓郁、酒体更稠,酒精度为12.6%(体积分数)、还原糖含量0.48g/100mL、乙酸乙酯含量259.16mg/L、乳酸乙酯含量192.12mg/L、虫草多糖含量0.46g/100mL、虫草素含量0.11g/100mL莲子粘液类物质含量15.2g/100mL,粘度为363mPa·s。The golden cordyceps lotus seed thick wine prepared by the above operation, compared with Example 1, has a richer ester aroma and a thicker wine body, with an alcohol content of 12.6% (volume fraction), a reducing sugar content of 0.48g/100mL, and an ethyl acetate content of 259.16 mg/L, ethyl lactate content 192.12mg/L, cordyceps polysaccharide content 0.46g/100mL, cordycepin content 0.11g/100mL lotus seed mucus content 15.2g/100mL, viscosity 363mPa·s.

Claims (8)

1.一种金色虫草莲子稠酒,其特征在于:是由下述方法制备的,制备的具体步骤为:1. a golden cordyceps lotus seed thick wine is characterized in that: it is prepared by the following method, and the concrete steps of preparation are: 1)将剥皮鲜莲子,洗净,常压蒸煮10-20min后,冷却至54-56℃;1) Wash the peeled fresh lotus seeds, cook at normal pressure for 10-20 minutes, and cool to 54-56°C; 2)将步骤1)的莲子放入池中,压实、密封,40-55℃保温48-72h;2) Put the lotus seeds of step 1) into the pond, compact and seal, and keep warm at 40-55°C for 48-72h; 3)将经步骤2)保温的莲子按重量份数为220-240的莲子与水1:1-1.5的比例加水磨浆,得莲子浆液,浆液中加入重量份数为0.024-0.031淀粉酶、0.031-0.037纤维素酶和0.019-0.027半纤维素酶,45-55℃保温酶解5-7h,板框压滤取汁,得莲子汁;3) adding water to the lotus seeds that have been insulated in step 2) according to the ratio of 220-240 parts by weight to lotus seeds and water 1:1-1.5, to obtain lotus seed slurry, adding 0.024-0.031 parts by weight of amylase, 0.031-0.037 cellulase and 0.019-0.027 hemicellulase, 45-55 ℃ heat preservation enzymolysis for 5-7h, plate and frame press filter to extract juice, to obtain lotus seed juice; 4)向步骤3)所得莲子汁中加入其质量1%葡萄糖、0.3%KH2PO4、0.15%MgSO4·7H2O、0.01%维生素B1、0.2%酵母膏,4%豆粕,灭菌,接种重量份数为50-60的虫草菌种子液,23℃培养7d,得虫草菌丝体发酵液;4) Add its quality 1% glucose, 0.3% KH2PO4, 0.15% MgSO4.7H2O, 0.01% vitamin B1, 0.2% yeast extract, 4% soybean meal to the lotus seed juice obtained in step 3), sterilize, and inoculate 50 parts by weight Cordyceps seed liquid at -60°C, cultured at 23°C for 7 days, to obtain Cordyceps mycelium fermentation liquid; 5)将步骤4)所得虫草菌丝体发酵液直接加入重量份数为80-100的白砂糖调整糖度;或将步骤4)所得虫草菌丝体发酵液经降膜蒸发器浓缩至原来体积的1/2加入重量份数为80-100的白砂糖调整糖度;5) directly add white granulated sugar of 80-100 parts by weight to the fermented liquid of Cordyceps mycelium obtained in step 4) to adjust the sugar content; or concentrate the fermented liquid of Cordyceps mycelium obtained in step 4) to the original volume by falling film 1/2 is added the white granulated sugar that is 80-100 parts by weight to adjust the sugar content; 调整糖度后18-25℃保温2h,加入重量份数为12.0-15.0的乳酸菌种子液,厌氧发酵36-48h后,再加入活化好的,重量份数为1.1-1.5的高活干酵母,10-15℃厌氧发酵15-20d后,过滤、澄清、加入重量份数为10-16的魔芋低聚糖调配、灌装、过滤膜除菌,得金色虫草莲子稠酒。After adjusting the sugar content, keep warm at 18-25°C for 2 hours, add lactic acid bacteria seed liquid with a weight ratio of 12.0-15.0, and after anaerobic fermentation for 36-48 hours, add activated high-activity dry yeast with a weight ratio of 1.1-1.5, After anaerobic fermentation at 10-15°C for 15-20 days, filter, clarify, add konjac oligosaccharides in parts by weight of 10-16 to prepare, fill, filter membrane to sterilize, and obtain golden cordyceps lotus seed thick wine. 2.根据权利要求1所述的一种金色虫草莲子稠酒,其特征在于:步骤2)中将步骤1)的莲子放入带夹层的塑料池中。2. a kind of golden cordyceps lotus seed thick wine according to claim 1 is characterized in that: in step 2), the lotus seed of step 1) is put into the plastic pond with interlayer. 3.根据权利要求1所述的一种金色虫草莲子稠酒,其特征在于:步骤4)中所述的虫草菌为中国工业微生物菌种保藏管理中心,保藏号CICC14014的蛹虫草,虫草种子液按如下方法制备而成:用试管固态培养基活化保藏的蛹虫草菌,28℃培养5d后,取面积为4cm2的菌丝块加入到100mL灭菌后的液态发酵培养基中,23℃培养3d,得蛹虫草菌种子液;3. a kind of golden cordyceps lotus seed thick wine according to claim 1, is characterized in that: the cordyceps fungus described in step 4) is China Industrial Microorganism Strain Preservation Management Center, the cordyceps militaris of preservation number CICC14014, cordyceps seed liquid Prepared by the following method: activate the preserved Cordyceps militaris with test tube solid medium, culture at 28°C for 5 days, take a piece of mycelium with an area of 4cm2 and add it to 100mL sterilized liquid fermentation medium, and cultivate at 23°C 3d, to obtain the Cordyceps militaris seed liquid; 所述固态培养基组成为:20%马铃薯汁1.0L,葡萄糖20g,KH2PO4 3g,MgSO4·7H2O 1.5g,维生素B1 0.1g,酵母膏2g,琼脂15g;The solid medium consists of: 1.0L of 20% potato juice, 20g of glucose, 3g of KH2PO4 , 1.5g of MgSO4 · 7H2O , 0.1g of vitamin B1, 2g of yeast extract, and 15g of agar; 所述液态发酵培养基组成为:20%马铃薯汁1.0L,葡萄糖20g,KH2PO4 3g,MgSO4·7H2O1.5g,萌芽米30g,豆粕40g,维生素B1 0.1g,酵母膏2g。The composition of the liquid fermentation medium is: 1.0L of 20% potato juice, 20g of glucose, 3g of KH 2 PO 4 , 1.5g of MgSO 4 7H 2 O, 30g of germinated rice, 40g of soybean meal, 0.1g of vitamin B 1 , and 2g of yeast extract . 4.根据权利要求1所述的一种金色虫草莲子稠酒,其特征在于:步骤5)中所述的乳酸菌种子液按如下方法制备而成:用试管MRS固态培养基活化乳酸菌,30℃培养28h,得活化后的乳酸菌,用接种环刮取菌丝接种到200mL的MRS液态培养基中培养制备一级乳酸菌种子液,28℃培养48h,将一级乳酸菌种子液按10%的接种量接种到2L的MRS液态发酵培养基扩大培养为二级乳酸菌种子液;4. A kind of golden cordyceps lotus seed thick wine according to claim 1, characterized in that: the lactic acid bacteria seed solution described in step 5) is prepared as follows: activate the lactic acid bacteria with a test tube MRS solid medium, and cultivate them at 30°C After 28 hours, the activated lactic acid bacteria were obtained, and the mycelium was scraped with an inoculation loop and inoculated into 200 mL of MRS liquid medium for cultivation to prepare a first-grade lactic acid bacteria seed solution, cultivated at 28°C for 48 hours, and the first-grade lactic acid bacteria seed solution was inoculated with a 10% inoculation amount To 2L of MRS liquid fermentation medium to expand the culture into secondary lactic acid bacteria seed liquid; 所述乳酸菌为中国工业微生物菌种保藏管理中心,保藏号CICC20014的短乳杆菌。The lactic acid bacterium is Lactobacillus brevis with the preservation number CICC20014 in China Industrial Microorganism Culture Collection Management Center. 5.根据权利要求1所述的一种金色虫草莲子稠酒,其特征在于:步骤5)中的中国工业微生物菌种保藏管理中心,保藏号CICC31121高活干酵母的活化方法为:按酵母与葡萄糖溶液1:10的质量比,将酵母加入到2%的葡萄糖溶液中,30℃活化15-30min的高活干酵母;5. a kind of golden cordyceps lotus seed thick wine according to claim 1 is characterized in that: in step 5), the activation method of the China Industrial Microorganism Strain Preservation Management Center, the preservation number CICC31121 high activity dry yeast is: press yeast and Glucose solution with a mass ratio of 1:10, adding yeast to 2% glucose solution, and activating high-activity dry yeast at 30°C for 15-30min; 所述魔芋低聚糖为,魔芋多糖经适当酶解所得分子量在3万~15万道尔顿的魔芋低聚糖。The konjac oligosaccharides are konjac oligosaccharides with a molecular weight of 30,000-150,000 Daltons obtained through appropriate enzymatic hydrolysis of konjac polysaccharides. 6.根据权利要求1所述的一种金色虫草莲子稠酒,其特征在于:各原料的重量份数为莲子230-240、虫草菌种子液53-55、白砂糖90-95、淀粉酶0.029-0.031、纤维素酶0.035-0.037、半纤维素酶0.025-0.027、乳酸菌种子液14.0-15.0、高活干酵母1.2-1.4、魔芋低聚糖13-16。6. A golden cordyceps lotus seed thick wine according to claim 1, characterized in that: the parts by weight of each raw material are lotus seeds 230-240, cordyceps seed liquid 53-55, white granulated sugar 90-95, amylase 0.029 -0.031, cellulase 0.035-0.037, hemicellulase 0.025-0.027, lactic acid bacteria seed liquid 14.0-15.0, high activity dry yeast 1.2-1.4, konjac oligosaccharides 13-16. 7.根据权利要求1所述的一种金色虫草莲子稠酒,其特征在于:各原料的重量份数为莲子230、虫草菌种子液55、白砂糖95、淀粉酶0.031、纤维素酶0.037、半纤维素酶0.027、乳酸菌种子液15.0、高活干酵母1.4、魔芋低聚糖16。7. A kind of golden cordyceps lotus seed thick wine according to claim 1, is characterized in that: the parts by weight of each raw material is lotus seed 230, cordyceps seed liquid 55, white granulated sugar 95, amylase 0.031, cellulase 0.037, Hemicellulase 0.027, lactic acid bacteria seed solution 15.0, high activity dry yeast 1.4, konjac oligosaccharide 16. 8.根据权利要求1所述的一种金色虫草莲子稠酒,其特征在于:各原料的重量份数为莲子235、虫草菌种子液54、白砂糖92、淀粉酶0.030、纤维素酶0.036、半纤维素酶0.026、乳酸菌种子液14.3、高活干酵母1.3、魔芋低聚糖14。8. A kind of golden cordyceps lotus seed thick wine according to claim 1, characterized in that: the parts by weight of each raw material are lotus seeds 235, cordyceps seed liquid 54, white granulated sugar 92, amylase 0.030, cellulase 0.036, Hemicellulase 0.026, lactic acid bacteria seed solution 14.3, high activity dry yeast 1.3, konjac oligosaccharide 14.
CN201610084153.8A 2016-02-06 2016-02-06 A kind of thick wine of gold cordyceps sinensis lotus seeds Active CN105567496B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610084153.8A CN105567496B (en) 2016-02-06 2016-02-06 A kind of thick wine of gold cordyceps sinensis lotus seeds

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610084153.8A CN105567496B (en) 2016-02-06 2016-02-06 A kind of thick wine of gold cordyceps sinensis lotus seeds

Publications (2)

Publication Number Publication Date
CN105567496A CN105567496A (en) 2016-05-11
CN105567496B true CN105567496B (en) 2019-10-08

Family

ID=55878132

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610084153.8A Active CN105567496B (en) 2016-02-06 2016-02-06 A kind of thick wine of gold cordyceps sinensis lotus seeds

Country Status (1)

Country Link
CN (1) CN105567496B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106318832A (en) * 2016-08-21 2017-01-11 桐庐富莲农业开发有限公司 Lotus seed wine
CN106367260A (en) * 2016-08-29 2017-02-01 陕西富士金医药保健品有限责任公司 Preparation method of dense cordyceps sinensis wine
CN113528277A (en) * 2020-04-14 2021-10-22 浙江同山醉美人酒业有限公司 Brewing process of high-yield lotus seed wine
CN111876285A (en) * 2020-09-16 2020-11-03 泸州窖龄年份酒业有限公司 Brewing method for solid-state fermentation of multi-flavor Luzhou-flavor liquor

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1454987A (en) * 2002-05-01 2003-11-12 汪志新 Medicinal-cultured cordyceps sinensis, and cultivating method and use thereof
CN101933640A (en) * 2010-07-14 2011-01-05 云南大理洱宝实业有限公司 Lotus seed-red jujube-walnut milk beverage and preparation method thereof
CN102388795A (en) * 2011-11-08 2012-03-28 付湘晋 Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds
CN103340453A (en) * 2013-07-08 2013-10-09 长沙理工大学 Method for producing low-sugar low-fat lotus paste through enzymolysis processing
CN103351972A (en) * 2013-07-09 2013-10-16 兴安县锦盛食用菌种植专业合作社 Method for preparing cordyceps militaris wine
CN104172097A (en) * 2014-07-09 2014-12-03 湖北工业大学 Method for preparing low-salt lotus seed fish sauce
CN104263585A (en) * 2014-10-11 2015-01-07 哈尔滨艾博雅食品科技开发有限公司 Manufacturing method of cordyceps militaris fermented wine
CN104664469A (en) * 2013-11-28 2015-06-03 天津美森科技有限公司 Ginkgo-lotus seed liquid and preparation method thereof
CN104862181A (en) * 2015-05-27 2015-08-26 河南科技学院 Preparation method for fermented artificial cordyceps crataequs pinnatifida bunges wine

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1454987A (en) * 2002-05-01 2003-11-12 汪志新 Medicinal-cultured cordyceps sinensis, and cultivating method and use thereof
CN101933640A (en) * 2010-07-14 2011-01-05 云南大理洱宝实业有限公司 Lotus seed-red jujube-walnut milk beverage and preparation method thereof
CN102388795A (en) * 2011-11-08 2012-03-28 付湘晋 Method of producing worm grass lotus seeds by virtue of solid state fermentation of lotus seeds
CN103340453A (en) * 2013-07-08 2013-10-09 长沙理工大学 Method for producing low-sugar low-fat lotus paste through enzymolysis processing
CN103351972A (en) * 2013-07-09 2013-10-16 兴安县锦盛食用菌种植专业合作社 Method for preparing cordyceps militaris wine
CN104664469A (en) * 2013-11-28 2015-06-03 天津美森科技有限公司 Ginkgo-lotus seed liquid and preparation method thereof
CN104172097A (en) * 2014-07-09 2014-12-03 湖北工业大学 Method for preparing low-salt lotus seed fish sauce
CN104263585A (en) * 2014-10-11 2015-01-07 哈尔滨艾博雅食品科技开发有限公司 Manufacturing method of cordyceps militaris fermented wine
CN104862181A (en) * 2015-05-27 2015-08-26 河南科技学院 Preparation method for fermented artificial cordyceps crataequs pinnatifida bunges wine

Also Published As

Publication number Publication date
CN105567496A (en) 2016-05-11

Similar Documents

Publication Publication Date Title
CN101248899B (en) Mango vinegar drink and preparation method thereof
CN101624560B (en) Preparation method of kelp fruit wine
CN104172100B (en) A kind of functional form fermented laminaria soy sauce and brew method thereof
CN109401908B (en) Anti-fatigue functional fruit vinegar beverage for teenagers and preparation method thereof
CN105567496B (en) A kind of thick wine of gold cordyceps sinensis lotus seeds
CN108893226A (en) A kind of method that cooperative fermentation preparation is rich in γ-aminobutyric acid mulberries drink
CN105647756B (en) A kind of thick wine of selenium-rich cordyceps mulberry health care
KR100899220B1 (en) Fermented Aloe and Manufacturing Method of Fermented Aloe and Functional food of Thereof Manufacturing
CN107114771A (en) A kind of high anthocyanidin highland barley ferment and its brewing method
CN107048361B (en) A kind of red jujube Dayun pomegranate peel compound enzyme and preparation method thereof
CN102351580B (en) Method for preparing black fungus nutrient solution by utilizing food processing residues
CN106085788B (en) Ginseng and medlar vinegar and preparation method and application thereof
CN105647755B (en) A kind of Se-rich lucid ganoderma mulberry health-care ice wine
CN106367284A (en) Liquid submerged fermentation technique of Chinese wolfberry/licorice root/apple health-care vinegar
CN108142765A (en) A kind of Cordyceps militaris composite enzyme drink preparation method of antifatigue and increasing immune power
CN102517188B (en) Preparation method of rice wine starter, capable of producing isomaltooligosaccharide during brewing process
CN102286339A (en) Method for producing enzymatic rice bran red koji wine
CN105505718B (en) A kind of thick wine of cordyceps sinensis mulberry juice health amber rich in DNJ
CN105695287B (en) A kind of selenium-rich agaric mulberry health care black vinegar
CN108934784A (en) A kind of mushroom high-yield culturing culture medium
CN109674019B (en) A soy sauce prepared from fructus Pruni Salicinae
CN116725179B (en) Herbal ferment capable of aiding sleep and soothing nerves and preparation method thereof
CN106136238A (en) A kind of manufacture method of spirit Fructus Jujubae ferment
CN105602821A (en) Lentinus edodes mycelia-black garlic vinegar
CN109370869A (en) A kind of bitter buckwheat ethylic acid fermented beverage and preparation method thereof rich in flavones

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant