CN105557690B - Rice paddy seed initiator and its application method under a kind of hypoxia stress - Google Patents

Abstract

The invention relates to a rice seed initiator under hypoxic stress and its application method, belonging to the technical field of rice planting. The rice seed initiator under hypoxic stress includes a seed initiator and a seed stress buffer, and each liter of the seed initiator contains the following components: PEG 80-120g, salicylic acid 0.1-0.6mg and jasmonic acid 0.1-0.5mg, Each liter of seed stress buffer contains the following components: glucose 20-30g, sucrose 20-30g, a-ketoglutaric acid 10-20g, fumaric acid 10-20g, L-glutamine 10-20g, ascorbic acid 10-20g and 25-35g of citric acid. On the basis of using seed priming to improve stress resistance, the present invention aims at possible limiting factors for seed germination under anaerobic stress, and configures different initiators and seed soaking agents to improve seed germination ability and seed germination rate.

Description

A rice seed initiator under hypoxic stress and its application method

technical field

The invention belongs to the technical field of rice planting, and in particular relates to a rice seed initiator under low-oxygen stress and its application method.

Background technique

Submergence/waterlogging is a major environmental factor limiting seed germination and seedling growth of direct-seeding rice. In actual production, due to reasons such as uneven land, poor drainage in the field, and unpredictable rainfall, direct-seeding rice seeds are often exposed to low-oxygen/anoxic flooding/waterlogging stress environments. Although rice, as a starchy seed, can provide a small amount of energy through ethanol fermentation without O2, in actual production, it still leads to problems such as poor germination rate and incomplete emergence of direct seeding rice, resulting in unstable direct seeding yield. The reason may be due to 1) under anaerobic stress, the efficiency of starch degradation and sugar utilization in seeds is low, and the seed germination rate is slow; 2) the stress period is long, and the small amount of energy supplied by anaerobic respiration cannot support seed germination for a long time; 3 ) Toxic effect of alcohol, a product of anaerobic respiration, on seed germination.

"Seed priming", also known as "osmotic regulation", is a seed treatment technology that controls the slow water absorption of seeds and the gradual drying back in the later stage to improve the germination rate of seeds. The principle is mainly to control the condition of the seeds to absorb water slowly, so that the seeds absorb water until the retention period, to ensure the smooth progress of seed germination metabolism, but to prevent the radicle from breaking through the seed coat, thereby reducing the damage of imbibition, and allowing the seeds to perform the physiological and biochemical metabolism and repair of pre-germination , and fully repair cell membranes, organelles, and DNA. A large number of studies have shown that the use of seed priming technology can activate various stress-resistant and defensive enzyme activities in seeds and improve their stress resistance.

Contents of the invention

Aiming at the problems existing in the prior art, the object of the present invention is to design and provide a technical scheme of a rice seed initiator and its application method under hypoxic stress.

The rice seed initiator under hypoxic stress is characterized in that it includes a seed initiator and a seed stress buffer, and each liter of the seed initiator contains the following components: PEG 80-120g, salicylic acid 0.1-0.6mg and jasmonic acid 0.1-0.5mg, each liter of seed stress buffer contains the following components: glucose 20-30g, sucrose 20-30g, a-ketoglutaric acid 10-20g, fumaric acid 10-20g, L-glutamine 10 ～20g, ascorbic acid 10～20g and citric acid 25～35g.

The rice seed initiator under hypoxic stress is characterized in that each liter of the seed initiator contains the following components: 90-110 g of PEG, 0.2-0.5 mg of salicylic acid and 0.2-0.4 mg of jasmonic acid .

The rice seed initiator under hypoxic stress is characterized in that each liter of the seed stress buffer contains the following components: 24-26g of glucose, 24-26g of sucrose, 14-26g of a-ketoglutarate 16g, fumaric acid 14-16g, L-glutamine 14-16g, ascorbic acid 14-16g and citric acid 28-32g.

The using method of the rice seed initiator under the described a kind of hypoxic stress environment is characterized in that comprising the following steps:

1) Select uniform and complete seeds, sterilize with 0.2% HgCl ₂ for 5 minutes, rinse with distilled water, put them in a beaker, add seed initiator, the amount of seeds per liter of seed initiator is 1-2kg, and artificially climate at 15°C Initiate treatment in the box for 36 hours under dark conditions;

2) Rinse the seeds quickly with distilled water after initiation, blot the surface moisture with filter paper, and then place them in a blast drying oven at 30°C to return to the original moisture content;

3) Before sowing, soak the seeds with the stress buffer for 10-24 hours, subject to no whitening, the amount of treated seeds per liter of stress buffer is 1-2kg, and the treated seeds can be sown in the field.

On the basis of using seed priming to improve stress resistance, the present invention aims at possible limiting factors for seed germination under anaerobic stress, and configures different initiators and seed soaking agents to improve seed germination ability and seed germination rate.

detailed description

The present invention will be further described below in conjunction with the examples.

Embodiment 1: the preparation of the rice seed initiator under a kind of hypoxic stress

Seed initiator: 100 g of PEG, 0.4 mg of salicylic acid and 0.3 mg of jasmonic acid were dissolved in 1 L of water to obtain a seed initiator.

Stress buffer: Dissolve 25 g of glucose, 25 g of sucrose, 15 g of a-ketoglutaric acid, 15 g of fumaric acid, 15 g of L-glutamine, 15 g of ascorbic acid and 30 g of citric acid in 1 L of water to obtain a stress buffer.

Embodiment 2: the preparation of the rice seed initiator under a kind of hypoxic stress

Seed initiator: Dissolve 80 g of PEG, 0.1 mg of salicylic acid and 0.1 mg of jasmonic acid in 1 L of water to obtain a seed initiator.

Stress buffer: 20 g of glucose, 20 g of sucrose, 10 g of a-ketoglutaric acid, 10 g of fumaric acid, 10 g of L-glutamine, 10 g of ascorbic acid and 25 g of citric acid were dissolved in 1 L of water to obtain a stress buffer.

Embodiment 3: the preparation of the rice seed initiator under a kind of hypoxic stress

Seed initiator: 120 g of PEG, 0.6 mg of salicylic acid and 0.5 mg of jasmonic acid were dissolved in 1 L of water to obtain a seed initiator.

Stress buffer: Dissolve 30 g of glucose, 30 g of sucrose, 20 g of a-ketoglutaric acid, 20 g of fumaric acid, 20 g of L-glutamine, 20 g of ascorbic acid and 35 g of citric acid in 1 L of water to obtain a stress buffer.

Embodiment 4: the preparation of the rice seed initiator under a kind of hypoxic stress

Seed initiator: Dissolve 90 g of PEG, 0.2 mg of salicylic acid and 0.2 mg of jasmonic acid in 1 L of water to obtain a seed initiator.

Stress buffer: 24 g of glucose, 24 g of sucrose, 16 g of a-ketoglutarate, 16 g of fumaric acid, 14 g of L-glutamine, 14 g of ascorbic acid and 32 g of citric acid were dissolved in 1 L of water to obtain a stress buffer.

Embodiment 5: the usage method of the rice seed initiator under a kind of low oxygen stress

1) Select uniform and complete seeds, sterilize with 0.2% HgCl ₂ for 5 minutes, rinse with distilled water, put them in a beaker, add seed initiator, the amount of seeds per liter of seed initiator is 1-2kg, and artificially climate at 15°C Initiate treatment in the box for 36 hours under dark conditions;

2) Rinse the seeds quickly with distilled water after initiation, blot the surface moisture with filter paper, and then place them in a blast drying oven at 30°C to return to the original moisture content;

3) Before sowing, soak the seeds with the stress buffer for 10-24 hours, subject to no whitening, the amount of treated seeds per liter of stress buffer is 1-2kg, and the treated seeds can be sown in the field.

Test example 1:

The test was carried out in 2015. The conventional seed soaking and accelerating germination treatment was CK, and the reagent (Example 1) and application method (Example 5) of the present invention were T. After sowing, the field surface kept a water layer of 10 cm until the end of the test. The germination rate of rice 7 days after sowing and the survival rate of seedlings 20 days after sowing were investigated (Table 1). The results showed that regardless of indica rice or japonica rice, there was no significant difference in germination rate between treatment T and control CK after 7 days of treatment, but with the advancement of growth process, there was a significant difference in seedling survival rate after 20 days, and the seedling survival rate under treatment T was significantly higher for comparison. Observations showed that under the control treatment, the late seedlings had rot and mildew, while under the treatment T, the symptoms were significantly reduced, which was related to the relieving effect of the carbonic acid in the stress agent.

Table 1 Effects of different treatments on seed production rate and seedling survival rate of direct-seeding rice under flooding stress

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Test example 2:

The test was carried out in 2015. The conventional seed soaking and accelerating germination treatment was CK, and the reagent (Example 3) and application method (Example 5) of the present invention were T. After sowing, the field surface kept a water layer of 10 cm until the end of the test. The germination rate of rice 7 days after sowing and the survival rate of seedlings 20 days after sowing were investigated (Table 2). The results showed that regardless of indica rice or japonica rice, there was no significant difference in the germination rate of treated seeds 7 days after treatment T and control CK, but with the advancement of the growth process, there was a significant difference in the survival rate of seedlings after 20 days, and the survival rate of seedlings under treatment T was significantly higher for comparison. Observations showed that under the control treatment, the late seedlings had rot and mildew, while under the treatment T, the symptoms were significantly reduced, which was related to the relieving effect of the carbonic acid in the stress agent.

Table 2 Effects of different treatments on seed production rate and seedling survival rate of direct-seeding rice under flooding stress

Claims (4)

1. a rice seed initiator under hypoxic stress, is characterized in that comprising seed initiator and seed stress buffer, every liter of seed initiator contains following components: PEG 80～120g, salicylic acid 0.1～0.6mg and Jasmonic acid 0.1~0.5mg, each liter of seed stress buffer contains the following components: glucose 20~30g, sucrose 20~30g, α-ketoglutarate 10~20g, fumaric acid 10~20g, L-glutamine 10~ 20g, ascorbic acid 10-20g and citric acid 25-35g. 2. the rice seed initiator under a kind of hypoxic stress environment as claimed in claim 1 is characterized in that described every liter of seed initiator contains following components: PEG 90～110g, salicylic acid 0.2～0.5mg and Jasmonic acid 0.2 ~ 0.4mg. 3. The rice seed initiator under a kind of hypoxic stress environment as claimed in claim 1, is characterized in that described every liter of seed stress buffer contains following components: glucose 24～26g, sucrose 24～26g, α- Ketoglutaric acid 14-16g, fumaric acid 14-16g, L-glutamine 14-16g, ascorbic acid 14-16g and citric acid 28-32g. 4. the using method of the rice seed initiator under a kind of hypoxic stress environment as claimed in claim 1,2 or 3, is characterized in that comprising the following steps: 1) Select uniform and complete seeds, sterilize with 0.2% HgCl ₂ for 5 minutes, rinse with distilled water, put them in a beaker, add seed initiator, the amount of seeds per liter of seed initiator is 1-2kg, and artificially climate at 15°C Initiate treatment in the box for 36 hours under dark conditions; 2) Rinse the seeds quickly with distilled water after initiation, blot the surface moisture with filter paper, and then place them in a blast drying oven at 30°C to return to the original moisture content; 3) Before sowing, soak the seeds with the stress buffer for 10-24 hours, subject to no whitening, the amount of treated seeds per liter of stress buffer is 1-2kg, and the treated seeds can be sown in the field.