CN105541057A - Resource utilization method for pig farm biogas slurry - Google Patents
Resource utilization method for pig farm biogas slurry Download PDFInfo
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Abstract
本发明涉及一种猪场沼液资源化利用的方法,即猪场的发酵沼液依次经过多级曝气过滤、接种光合细菌并培养、接种枝角类滤食光合细菌并收集枝角类、滤液接种单细胞藻类并培养、接种枝角类滤食单细胞藻类并收集枝角类及处理后达标水回用或排放环节,处理过程中先利用光合细菌降解沼液中的有机物并生成菌体,后利用微藻吸收水体中的氮磷等营养盐并生成藻体,利用枝角类非选择性滤食水体中的菌和藻。本发明的猪场沼液资源化利用方法可将沼液处理过程中生成的光合细菌、单细胞藻类和枝角类用于水产养殖和饵料,借助食物链将沼液中的污染物质实现资源化利用,具有投入低、能耗低、可产生明显的经济和生态效益的特点。
The invention relates to a method for resource utilization of pig farm biogas slurry, that is, the fermented biogas slurry of pig farms is sequentially passed through multi-stage aeration and filtration, inoculated and cultivated with photosynthetic bacteria, inoculated with cladocera filter-feeding photosynthetic bacteria and collected cladocera, The filtrate is inoculated with single-cell algae and cultured, inoculated with cladocera filter-feeding single-cell algae and collected cladocera, and the treated water reaches the standard for reuse or discharge. During the treatment process, photosynthetic bacteria are first used to degrade the organic matter in the biogas slurry and generate bacteria. , and then use microalgae to absorb nutrients such as nitrogen and phosphorus in the water body and generate algae, and use cladocera to filter-feed bacteria and algae in the water body non-selectively. The pig farm biogas slurry resource utilization method of the present invention can use the photosynthetic bacteria, unicellular algae and cladocera generated in the biogas slurry treatment process for aquaculture and bait, and realize resource utilization of the pollutants in the biogas slurry by means of the food chain, It has the characteristics of low investment, low energy consumption, and can produce obvious economic and ecological benefits.
Description
技术领域technical field
本发明涉及猪场沼液处理技术领域,具体地说,是一种猪场沼液资源化利用的方法。The invention relates to the technical field of pig farm biogas slurry treatment, in particular to a method for resource utilization of pig farm biogas slurry.
背景技术Background technique
随着人口的增长、人民生活水平的提高和社会经济的快速发展,人类对动物性蛋白的需求也逐步提高。解决人类动物性蛋白短缺关键靠养殖,发展畜禽和水产养殖是解决动物性蛋白短缺的重要措施。在规模化畜禽养殖过程中产生的废气、废液和废渣,对周边的生态环境保护往往造成很大的压力。养殖污染已成为农业农村环境污染的主要来源。养殖污水和液态排泄物是集约化畜禽养殖场污染物无害化处理的难点,其主要污染物是有机物,氮和磷。目前规模化畜禽养殖场的污水通常采用沼气池厌氧发酵处理(畜禽养殖业污染治理工程技术规范),但产生的数量巨大的沼液中仍然含有高浓度的氮磷等营养盐。以生猪养殖场为例,其养殖污水经沼气池厌氧发酵处理后沼液中的污染物浓度依然很高,BOD5、氨氮和总磷的浓度分别高达389mg/L、578.5mg/L和32.9mg/L,与国家畜禽养殖业污染物排放标准(DB33/593-2005;GB/T18596-2001)仍有很大的距离(标准中集约化畜禽养殖污水中BOD5、总氮和总磷的最高允许日均排放浓度分别为150mg/L、80mg/L和8mg/L)。将产生的沼液直排到水体,势必会导致自然水体严重富营养化。因此,沼液仍然需要二次处理。畜禽养殖场沼液有两种常规的处理模式,一种为“达标排放”模式,一种为“综合利用”模式(《规模化畜禽养殖场沼气工程设计规程》(NY/T1168))。达标排放模式下,畜禽废水在经厌氧消化处理后,必须再经过适当的好氧处理或自然处理等,达到规定的环保标准排放或回用。这种模式的工程造价和运行费均相对较高,且为非资源化利用模式。“综合利用”模式是将沼液排入农田依靠农作物自然吸收的方式进行,是低效率的资源化利用模式。这样的处理方式随着社会经济的发展,其弊端日渐显现:一是处理效果受作物生长季节的影响难以保证;二是农业产业结构的调整和城镇化开发的加速使得消纳沼液的耕地不足,因此,采用其他更高效方式开展沼液的深度处理显得尤为必要。如何净化沼液,越来越成为规模化畜禽养殖场可持续生产的制约因素。With the growth of population, the improvement of people's living standards and the rapid development of social economy, the human demand for animal protein is also gradually increasing. The key to solving the shortage of human animal protein depends on breeding, and the development of livestock and aquaculture is an important measure to solve the shortage of animal protein. The waste gas, waste liquid and waste residue produced in the process of large-scale livestock and poultry breeding often cause great pressure on the surrounding ecological environment protection. Breeding pollution has become the main source of agricultural and rural environmental pollution. Breeding sewage and liquid excrement are the difficulties in the harmless treatment of pollutants in intensive livestock and poultry farms. The main pollutants are organic matter, nitrogen and phosphorus. At present, the sewage of large-scale livestock and poultry farms is usually treated by anaerobic fermentation in biogas tanks (Technical Specifications for Pollution Control Engineering of Livestock and Poultry Breeding Industry), but the huge amount of biogas slurry still contains high concentrations of nutrients such as nitrogen and phosphorus. Taking pig farms as an example, the concentration of pollutants in the biogas slurry is still high after the aquaculture sewage is treated by anaerobic fermentation in the biogas tank, and the concentrations of BOD 5 , ammonia nitrogen and total phosphorus are as high as 389mg/L, 578.5mg/L and 32.9 mg/L, there is still a large distance from the national livestock and poultry breeding pollutant discharge standard (DB33/593-2005; GB/T18596-2001) (BOD 5 , total nitrogen and total The maximum allowable daily average discharge concentration of phosphorus is 150mg/L, 80mg/L and 8mg/L respectively). Directly discharging the generated biogas slurry to the water body will inevitably lead to serious eutrophication of the natural water body. Therefore, biogas slurry still needs secondary treatment. There are two conventional treatment modes for biogas slurry in livestock and poultry farms, one is the "standard discharge" mode and the other is the "comprehensive utilization" mode ("Large-scale Livestock and Poultry Farm Biogas Engineering Design Regulations" (NY/T1168)) . Under the standard discharge mode, after anaerobic digestion treatment, livestock and poultry wastewater must undergo appropriate aerobic treatment or natural treatment, etc., to meet the specified environmental protection standards for discharge or reuse. The project cost and operation cost of this mode are relatively high, and it is a non-resource utilization mode. The "comprehensive utilization" mode is to discharge biogas slurry into the farmland and rely on the natural absorption of crops, which is a low-efficiency resource utilization mode. With the development of social economy, the disadvantages of this treatment method are becoming more and more obvious: first, the treatment effect is difficult to guarantee due to the influence of crop growing season; second, the adjustment of agricultural industrial structure and the acceleration of urbanization development make the arable land for digesting biogas slurry insufficient , Therefore, it is particularly necessary to adopt other more efficient ways to carry out advanced treatment of biogas slurry. How to purify biogas slurry has increasingly become a restrictive factor for the sustainable production of large-scale livestock and poultry farms.
光合细菌(PhotosyntheticBacteria,简称PSB)是地球上出现最早、自然界中普遍存在、具有原始光能合成体系的原核生物,是在厌氧条件下进行不放氧光合作用的细菌的总称,可以光作为能源、能在厌氧光照或好氧黑暗条件下利用自然界中的有机物、硫化物、氨等作为供氢体兼碳源进行光合作用的微生物,在自然界的物质净化过程中发挥重要作用,特别对降解水体中的有机物效果明显,起到净化水质的作用。Photosynthetic bacteria (Photosynthetic Bacteria, referred to as PSB) are the earliest prokaryotic organisms that appeared on the earth, are ubiquitous in nature, and have a primitive light energy synthesis system. , Microbes that can use organic matter, sulfide, ammonia, etc. in nature as hydrogen donors and carbon sources for photosynthesis under anaerobic light or aerobic dark conditions, play an important role in the purification process of natural substances, especially for degradation The organic matter in the water body has an obvious effect and plays a role in purifying the water quality.
微藻作为一类低等光合自养型生物,种类丰富。微藻细胞能以光能为能源,从水体中吸收硝氮、氨氮及磷酸盐等营养物质用于合成细胞组分。已有的实验研究表明,不同微藻对水体中不同形式的氮、磷等营养盐的耐受、吸收和利用能力不同。在利用微藻净化污水的实践方面,1957年,Oswald等首次将微藻作为一种取代污水处理中的活性污泥的生物系统,奠定了藻类污水生物处理技术的基础。此后,人们越来越重视细菌与藻类相结合的应用,建立在人工模式水体藻-菌共生自净原理基础上的氧化塘技术得到广泛的应用和发展。近年来将畜禽污水净化耦合微藻高附加值产品生产的研究也有报道,主要集中在利用畜禽污水开展微藻生物柴油生产的领域。胡洪营等(2009)探讨了基于微藻细胞培养的水质深度净化与高价值生物质生产耦合技术。Sydney等(2010)开创性的将微藻污水处理和微藻生物柴油生产相耦合。胡洪营和李鑫(2010)分析了利用污水资源生产微藻生物柴油的关键技术及潜力分析。As a class of low-level photoautotrophic organisms, microalgae are rich in species. Microalgal cells can use light energy as energy to absorb nutrients such as nitrate nitrogen, ammonia nitrogen, and phosphate from water to synthesize cell components. Existing experimental studies have shown that different microalgae have different tolerance, absorption and utilization capabilities to different forms of nitrogen, phosphorus and other nutrients in water. In terms of the practice of using microalgae to purify sewage, in 1957, Oswald et al. first used microalgae as a biological system to replace activated sludge in sewage treatment, laying the foundation for algae sewage biological treatment technology. Since then, people have paid more and more attention to the application of the combination of bacteria and algae, and the oxidation pond technology based on the principle of algae-bacteria symbiotic self-purification in artificial model water has been widely used and developed. In recent years, studies on the coupling of livestock and poultry sewage purification to the production of high value-added products from microalgae have also been reported, mainly focusing on the field of microalgae biodiesel production using livestock and poultry sewage. Hu Hongying et al. (2009) discussed the coupling technology of deep water purification and high-value biomass production based on microalgae cell culture. Sydney et al. (2010) pioneered the coupling of microalgae wastewater treatment and microalgae biodiesel production. Hu Hongying and Li Xin (2010) analyzed the key technology and potential analysis of using sewage resources to produce microalgae biodiesel.
枝角类,隶属于节肢动物门,甲壳纲,鳃足亚纲,枝角目的小型甲壳类,广泛分布于淡水、海水和内陆半咸水中,是水产动物的优质天然饵料,其中的某些种类具有较强的耐污性,可非选择性滤食水体中的有机碎屑、为藻细胞和细菌,从而将水体中难以采收的微小营养物质转变成小颗粒易采收的饵料Cladocera, belonging to the phylum Arthropoda, Crustacea, Branchopod, and small crustaceans of Cladocera, are widely distributed in fresh water, sea water and inland brackish water, and are high-quality natural bait for aquatic animals. Some of them have Strong pollution resistance, non-selective filter-feeding of organic detritus, algae cells and bacteria in the water body, so as to transform the micro nutrients that are difficult to harvest in the water body into small particles that are easy to harvest
综上所述,开发可循环利用、高效、低成本的污水处理技术是规模化畜禽养殖可持续发展的重要措施。将畜禽养殖废弃物综合利用,变废为宝,是减少畜禽养殖废弃物随意排放,从根本上破解畜禽养殖污染防治难题的根本出路。To sum up, the development of recyclable, efficient and low-cost sewage treatment technology is an important measure for the sustainable development of large-scale livestock and poultry farming. The comprehensive utilization of livestock and poultry breeding waste and turning waste into treasure is the fundamental way to reduce the random discharge of livestock and poultry breeding waste and fundamentally solve the problem of livestock and poultry breeding pollution prevention and control.
发明内容Contents of the invention
本发明的目的是针对现有技术中的不足,提供一种猪场沼液资源化利用的方法,而关于这种猪场沼液资源化利用的方法目前还未见报道。The purpose of the present invention is to provide a method for resource utilization of pig farm biogas slurry in view of the deficiencies in the prior art, but there is no report about this method for resource utilization of pig farm biogas slurry.
为实现上述目的,本发明采取的技术方案是:For realizing above-mentioned object, the technical scheme that the present invention takes is:
一种猪场沼液资源化利用的方法,其特征在于,即猪场沼气池中流出的发酵沼液依次经过多级曝气过滤、接种光合细菌并培养、接种枝角类滤食光合细菌并收集枝角类、滤液接种单细胞藻类并培养、接种枝角类滤食单细胞藻并收集枝角类及处理后达标水排放或回用等环节,处理过程中先利用光合细菌降解沼液中的有机物并生成菌体,后利用微藻吸收沼液中的氮磷等营养盐并生成藻细胞,再利用枝角类非选择性滤食处理过程中形成的有机碎屑、光合细菌和单细胞藻类,生产的枝角类、光合细菌、单细胞藻类可用于水产养殖或饵料,从而实现沼液中污染物质的资源化利用。A method for resource utilization of pig farm biogas slurry, characterized in that the fermented biogas slurry flowing out of the pig farm biogas digester is sequentially passed through multi-stage aeration and filtration, inoculated and cultivated with photosynthetic bacteria, inoculated with cladocera filter-feeding photosynthetic bacteria and Collect cladocera, inoculate the filtrate with unicellular algae and cultivate them, inoculate cladocera filter-feeding unicellular algae and collect cladocera, and discharge or reuse the treated water that meets the standard. During the treatment process, photosynthetic bacteria are used to degrade the biogas slurry Then use microalgae to absorb nutrients such as nitrogen and phosphorus in the biogas slurry and generate algal cells, and then use organic debris, photosynthetic bacteria and single cells formed during the non-selective filter feeding process of Cladocera Algae, Cladocera, photosynthetic bacteria, and single-cell algae produced can be used in aquaculture or bait, so as to realize the resource utilization of pollutants in biogas slurry.
进一步地,猪场沼气池中流出的发酵沼液首先经过多级曝气过滤,采用的多级曝气过滤的不锈钢筛网规格依次为200目,300目、400目和500目,筛网呈一定角度倾斜,在筛网底部安装纳米曝气管,在曝气的作用下将沼液中的部分氨氮吹脱,同时防止筛网网眼堵塞。Furthermore, the fermented biogas liquor flowing out of the pig farm biogas digester is firstly filtered through multi-stage aeration, and the specifications of the multi-stage aeration and filtration stainless steel screens used are 200 mesh, 300 mesh, 400 mesh and 500 mesh in sequence. It is inclined at a certain angle, and nano-aeration tubes are installed at the bottom of the screen to blow off part of the ammonia nitrogen in the biogas slurry under the action of aeration, while preventing the mesh of the screen from being blocked.
进一步地,经过多级曝气过滤的沼液中接种光合细菌以降解和吸收有机物,光合细菌的菌种为红假单胞菌属Rhodopseudomonas、红螺菌属Rhodospirillum和红细菌属Rhodobacter的种类,光合细菌的初始接种量与多级曝气过滤沼液的比例为3:7~5:5,在透明的有机玻璃柱或柱状柔性塑料薄膜袋中进行5~10天培养后,维持一定的沼液流入和光合细菌菌液流出速度实行连续培养,流入流出的速度根据光合细菌生长情况调整。Further, photosynthetic bacteria are inoculated in the biogas slurry filtered through multistage aeration to degrade and absorb organic matter. The species of photosynthetic bacteria are Rhodopseudomonas, Rhodospirillum and Rhodobacter. The ratio of the initial inoculum of bacteria to the biogas slurry filtered by multi-stage aeration is 3:7-5:5. The inflow and outflow speeds of photosynthetic bacteria liquid are continuously cultivated, and the inflow and outflow speeds are adjusted according to the growth of photosynthetic bacteria.
进一步地,培养好的光合细菌一部分可根据需要直接采收用于水产养殖生产,另一部分流出的光合细菌菌液进入方形培养水槽后,接种枝角类,利用枝角类非选择性滤食其中的有机碎屑和光合细菌,从而去除水体中的有机和无机物颗粒,提升水体透明度,所接种的枝角类的种类为裸腹蚤属的似亲裸腹蚤Moinaaffinis、蚤属的大型蚤Daphniamagna和象鼻蚤属的象鼻蚤Bosminalongirostris。Further, part of the cultured photosynthetic bacteria can be directly harvested for aquaculture production according to needs, and the other part of the outflowing photosynthetic bacteria liquid enters the square culture tank, inoculates cladocera, and utilizes cladocera to filter-feed them non-selectively. The organic detritus and photosynthetic bacteria in the water body can remove the organic and inorganic particles in the water body and improve the transparency of the water body. The species of Cladocera inoculated are Moinaaffinis of the genus Pyrophylla and Daphniamagna of the genus and the weevil flea Bosminalongirostris.
进一步地,所培养的枝角类可通过过滤采收装置富集,富集的枝角类可用作水产动物种苗或观赏鱼的活饵料,也可循环用于滤食单细胞藻类。Further, the cultivated Cladocera can be enriched through a filter harvesting device, and the enriched Cladocera can be used as live bait for aquatic animal seedlings or ornamental fish, and can also be recycled for filter-feeding single-cell algae.
进一步地,经过枝角类一次滤食的沼液,接种单细胞藻类以吸收水体中的氮磷等营养盐,单细胞藻类的藻种为小球藻属Chlorella、卵囊藻属Oocystis、绿球藻属Chlorococcum和栅藻属Scenedesmus的种类,单细胞的初始接种量与经过枝角类一次滤食的沼液的比例为1:1~5,在透明的有机玻璃柱或柱状柔性塑料薄膜袋中进行5~10天充气培养后,维持一定的沼液流入和单细胞藻液流出速度实行连续培养,流入流出的速度根据单细胞生长情况和水体中氮磷的含量调整。Further, through biogas slurry fed by Cladocera once, unicellular algae are inoculated to absorb nutrients such as nitrogen and phosphorus in the water body. The algae of the genus Chlorococcum and the genus Scenedesmus, the ratio of the initial inoculum of single cells to the biogas slurry that has been filtered by Cladocera is 1:1~5, in a transparent plexiglass column or a columnar flexible plastic film bag After 5-10 days of aerated culture, maintain a certain biogas slurry inflow and single-cell algae liquid outflow speed to implement continuous culture, and the inflow and outflow speed is adjusted according to the growth of single cells and the content of nitrogen and phosphorus in the water.
进一步地,培养好的藻液一部分可根据需要直接采收用于水产养殖生产,另一部分流出的藻液进入方形培养水槽后,接种枝角类,利用枝角类非选择性的滤食特性二次滤食其中的有机碎屑和单细胞藻类,进一步净化水体,所接种的枝角类的种类为裸腹蚤属的似亲裸腹蚤Moinaaffinis、蚤属的大型蚤Daphniamagna和象鼻蚤属的象鼻蚤Bosminalongirostris。Further, part of the cultivated algae liquid can be directly harvested for aquaculture production according to needs, and the other part of the outflowed algae liquid enters the square culture tank and is inoculated with cladocera, utilizing the non-selective filter-feeding characteristics of cladocera. The organic detritus and unicellular algae among them are filtered for the second time, and the water body is further purified. The species of Cladocera inoculated are Moinaaffinis of the genus Pleia, Daphniamagna of the genus Daphniamagna, and the genus Elephantis Elephant flea Bosminalongirostris.
进一步地,经过枝角类二次滤食的沼液,经检测符合畜禽养殖污水排放标准后,可进行处理达标水排放或回用作为猪舍的冲粪水,若检测不达排放标准则进行重复处理。Furthermore, the biogas slurry that has undergone the secondary filtering of Cladocera can be treated and discharged as up-to-standard water after being tested to meet the sewage discharge standards for livestock and poultry farming, or reused as manure flushing water for pig houses. If the test does not meet the discharge standards, then Do repeated processing.
本发明优点在于:The present invention has the advantage that:
1、本发明的猪场沼液资源化利用方法可将沼液处理过程中生成的光合细菌、单细胞藻类和枝角类用于水产养殖和饵料,借助食物链将沼液中的污染物质实现资源化利用,具有投入低、能耗低、可产生明显的经济和生态效益的特点;1. The pig farm biogas slurry resource utilization method of the present invention can use the photosynthetic bacteria, unicellular algae and cladocera generated in the biogas slurry treatment process for aquaculture and bait, and realize resource utilization of the pollutants in the biogas slurry by means of the food chain Utilization has the characteristics of low investment, low energy consumption, and can produce obvious economic and ecological benefits;
2、传统的废水治理工程只能削减污染物的排放量与排放强度,通常少有产品产出,只能产生环境、生态和社会效益,难以产生经济效益。这种只投入无产出的模式导致企业对环保投入不积极,而本发明将污水处理与活饵料生产耦合起来,不但能实现污水有效处理,且能降低活饵料生产的成本,实现环境的可持续及资源的循环利用,符合国家发展生态农业、绿色农业和循环农业的趋势;2. Traditional wastewater treatment projects can only reduce the discharge volume and intensity of pollutants, and usually produce few products, which can only produce environmental, ecological and social benefits, and are difficult to produce economic benefits. This mode of only investing but not producing results in enterprises being inactive in environmental protection investment, and the present invention couples sewage treatment with live bait production, not only can realize effective treatment of sewage, but also can reduce the cost of live bait production and realize environmental sustainability and resource recycling, in line with the national trend of developing ecological agriculture, green agriculture and circular agriculture;
3、畜禽养殖污水既是严重的污染源,也是宝贵的资源。而本发明在传统畜禽养殖污水处理工艺中引入光合细菌、微藻和枝角类,通过光合细菌-微藻-枝角类食物链将猪场沼液净化及活饵料生产相耦合,利用猪场沼液中高浓度的氮、磷、有机物等营养盐培养光合细菌和微藻,将所培养光合细菌和微藻通过枝角类滤食生产活饵料用于动物养殖生产,从而实现环境保护及资源的高效循环利用,保障生产和生态的可持续发展。3. The sewage from livestock and poultry breeding is not only a serious source of pollution, but also a valuable resource. However, the present invention introduces photosynthetic bacteria, microalgae and Cladocera into the traditional livestock and poultry breeding sewage treatment process, and couples the pig farm biogas slurry purification and live bait production through the photosynthetic bacteria-microalgae-Cladocera food chain. Photosynthetic bacteria and microalgae are cultivated with high-concentration nitrogen, phosphorus, organic matter and other nutrients in the liquid, and the cultured photosynthetic bacteria and microalgae are filtered through cladocerae to produce live bait for animal breeding and production, so as to achieve environmental protection and resource efficiency Recycling ensures the sustainable development of production and ecology.
附图说明Description of drawings
附图1是本发明的一种猪场沼液资源化利用的方法流程示意图。Accompanying drawing 1 is a schematic flow chart of a method for resource utilization of swine farm biogas slurry according to the present invention.
具体实施方式detailed description
实施例1Example 1
在上海崇明某大型标准化生猪养殖企业开展基于光合细菌-微藻-枝角类的猪场沼液资源化利用和净化处理。通过在沼液池旁构建200平米温室大棚并在大棚中安装培养水槽、透明柱状有机玻璃柱、柱状柔性塑料薄膜袋、多级曝气过滤装置、浮游动物富集采收装置、管道及充气设施等,开展猪场沼液资源化利用和净化处理。In a large-scale standardized pig breeding enterprise in Chongming, Shanghai, the resource utilization and purification treatment of pig farm biogas slurry based on photosynthetic bacteria-microalgae-cladocera was carried out. By building a 200-square-meter greenhouse next to the biogas slurry tank and installing a culture tank, a transparent columnar plexiglass column, a columnar flexible plastic film bag, a multi-stage aeration filter device, a zooplankton enrichment and collection device, pipelines and inflatable facilities etc., to carry out resource utilization and purification treatment of pig farm biogas slurry.
请参照图1,图1是本发明的一种猪场沼液资源化利用的方法流程示意图。一种猪场沼液资源化利用的方法,主要做法是猪场沼气池中流出的发酵沼液依次经过多级曝气过滤、接种光合细菌并培养、接种枝角类滤食光合细菌并收集枝角类、滤液接种单细胞藻类并培养、接种枝角类滤食单细胞藻并收集枝角类及处理后达标水排放或回用等环节。处理过程中先利用光合细菌降解沼液中的有机物并生成菌体,后利用微藻吸收沼液中的氮磷等营养盐并生成藻细胞,再利用枝角类非选择性滤食处理过程中形成的有机碎屑、光合细菌和单细胞藻类,生产的枝角类、光合细菌、单细胞藻类可用于水产养殖或饵料,从而实现沼液中污染物质的资源化利用。Please refer to FIG. 1 , which is a schematic flowchart of a method for resource utilization of swine farm biogas slurry according to the present invention. A method for resource utilization of pig farm biogas slurry, the main method is that the fermented biogas slurry flowing out of the pig farm biogas tank is sequentially filtered through multi-stage aeration, inoculated with photosynthetic bacteria and cultured, inoculated with cladocera filter-feeding photosynthetic bacteria and collected branches Inoculation and cultivation of unicellular algae with cladocera and filtrate, inoculation of cladocera with filter-feeding unicellular algae and collection of cladocera, discharge or reuse of treated water up to standard, etc. In the process of treatment, photosynthetic bacteria are used to degrade the organic matter in the biogas slurry and generate bacteria, and then microalgae are used to absorb nutrients such as nitrogen and phosphorus in the biogas slurry and generate algal cells, and then use cladocera non-selective filter feeding during the treatment process The formed organic debris, photosynthetic bacteria and unicellular algae can be used for aquaculture or bait, so as to realize the resource utilization of pollutants in the biogas slurry.
其中,沼气池流出的发酵沼液首先经过多级曝气过滤,采用的多级曝气过滤的不锈钢筛网规格依次为200目,300目、400目和500目,筛网呈一定角度倾斜,在筛网底部安装纳米曝气管,在曝气的作用下将沼液中的部分氨氮吹脱,同时防止筛网网眼堵塞。Among them, the fermented biogas slurry flowing out of the biogas digester is firstly filtered by multi-stage aeration. The specifications of the stainless steel screen mesh used for multi-stage aeration filtration are 200 mesh, 300 mesh, 400 mesh and 500 mesh in turn. The screen mesh is inclined at a certain angle. Nano-aeration tubes are installed at the bottom of the screen to blow off part of the ammonia nitrogen in the biogas slurry under the action of aeration, while preventing the mesh of the screen from being blocked.
其中,经过多级曝气过滤的沼液中接种光合细菌以降解和吸收有机物,光合细菌的菌种为红假单胞菌属Rhodopseudomonas、红螺菌属Rhodospirillum和红细菌属Rhodobacter的种类,光合细菌的初始接种量与多级曝气过滤沼液的比例为3:7~5:5,在透明的有机玻璃柱或柱状柔性塑料薄膜袋中进行5~10天培养后,维持一定的沼液流入和光合细菌菌液流出速度实行连续培养,流入流出的速度根据光合细菌生长情况调整。Among them, photosynthetic bacteria are inoculated into the biogas slurry that has been filtered by multi-stage aeration to degrade and absorb organic matter. The species of photosynthetic bacteria are Rhodopseudomonas, Rhodospirillum and Rhodobacter. The ratio of the initial inoculation amount to the biogas slurry filtered by multi-stage aeration is 3:7-5:5, and after 5-10 days of cultivation in a transparent plexiglass column or columnar flexible plastic film bag, a certain amount of biogas slurry inflow is maintained. Carry out continuous culture with the outflow speed of photosynthetic bacteria, and the inflow and outflow speed is adjusted according to the growth of photosynthetic bacteria.
其中,培养好的光合细菌一部分可根据需要直接采收用于水产养殖生产,另一部分流出的光合细菌菌液进入方形培养水槽后,接种枝角类,利用枝角类非选择性滤食其中的有机碎屑和光合细菌,从而去除水体中的有机和无机物颗粒,提升水体透明度,所接种的枝角类的种类为裸腹蚤属的似亲裸腹蚤Moinaaffinis、蚤属的大型蚤Daphniamagna和象鼻蚤属的象鼻蚤Bosminalongirostris。Among them, part of the cultured photosynthetic bacteria can be directly harvested for aquaculture production according to needs, and the other part of the outflowing photosynthetic bacteria liquid enters the square culture tank, inoculates cladocera, and utilizes cladocera to non-selectively filter feed them. Organic detritus and photosynthetic bacteria, so as to remove organic and inorganic particles in the water body and improve the transparency of the water body. The species of Cladocera inoculated are Moinaaffinis of the genus Pleia, Daphniamagna of the genus Daphniamagna and Weevil flea Bosminalongirostris.
其中,所培养的枝角类可通过过滤采收装置富集,富集的枝角类可用作水产动物种苗或观赏鱼的活饵料,也可循环用于滤食单细胞藻类。Wherein, the cultivated Cladocera can be enriched through the filter harvesting device, and the enriched Cladocera can be used as live bait for aquatic animal seedlings or ornamental fish, and can also be recycled for filter-feeding single-celled algae.
其中,经过枝角类一次滤食的沼液,接种单细胞藻类以吸收水体中的氮磷等营养盐,单细胞藻类的藻种为小球藻属Chlorella、卵囊藻属Oocystis、绿球藻属Chlorococcum和栅藻属Scenedesmus的种类,单细胞的初始接种量与经过枝角类一次滤食的沼液的比例为1:1~5,在透明的有机玻璃柱或柱状柔性塑料薄膜袋中进行5~10天充气培养后,维持一定的沼液流入和单细胞藻液流出速度实行连续培养,流入流出的速度根据单细胞生长情况和水体中氮磷的含量调整。Among them, the biogas slurry that has been filtered by Cladocera once is inoculated with unicellular algae to absorb nutrients such as nitrogen and phosphorus in the water body. The algae species of unicellular algae are Chlorella, Oocystis, and Chlorella It belongs to the species of Chlorococcum and Scenedesmus, the ratio of the initial inoculum of single cells to the biogas slurry that has been filtered by Cladocera is 1:1~5, and it is carried out in a transparent plexiglass column or a columnar flexible plastic film bag After 5-10 days of aerated culture, maintain a certain biogas slurry inflow and single-cell algae liquid outflow speed to implement continuous culture. The inflow and outflow speed is adjusted according to the growth of single cells and the nitrogen and phosphorus content in the water.
其中,培养好的藻液一部分可根据需要直接采收用于水产养殖生产,另一部分流出的藻液进入方形培养水槽后,接种枝角类,利用枝角类非选择性的滤食特性二次滤食其中的有机碎屑和单细胞藻类,进一步净化水体,所接种的枝角类的种类为裸腹蚤属的似亲裸腹蚤Moinaaffinis、蚤属的大型蚤Daphniamagna和象鼻蚤属的象鼻蚤Bosminalongirostris。Among them, part of the cultured algae liquid can be directly harvested for aquaculture production according to needs, and the other part of the outflowed algae liquid enters the square culture tank and is inoculated with Cladocera, using the non-selective filter-feeding characteristics of Cladocera for secondary feeding. Filter-feed the organic debris and single-celled algae in it to further purify the water body. The species of Cladocera inoculated are Moinaaffinis of the genus Pyrophora, Daphniamagna of the genus Daphniamagna, and elephants of the genus Elephantrum Nasal flea Bosminalongirostris.
其中,经过枝角类二次滤食的沼液,经检测符合畜禽养殖污水排放标准后,可进行处理达标水排放或回用作为猪舍的冲粪水,若检测不达排放标准则进行重复处理。Among them, the biogas slurry that has undergone the secondary filtration of cladocera can be treated and discharged after being tested to meet the sewage discharge standards for livestock and poultry breeding, or reused as flushing water for pig houses. If it does not meet the discharge standards, it can be treated Duplicate processing.
实施效果:Implementation Effect:
2015年通过本发明方法进行猪场沼液资源化利用和净化处理,共处理了沼液约450立方米,其中生产了光合细菌菌液75立方米,单细胞藻液50立方米,鲜活枝角类355公斤,获得达标排放水体约310立方米,排放水的主要水质指标如下:化学耗氧量<300mg/L,氨氮<50mg/L,总磷<5mg/L,pH6.8-7.5,透明度>40cm。In 2015, the pig farm biogas slurry resource utilization and purification treatment was carried out through the method of the present invention, and a total of about 450 cubic meters of biogas slurry was processed, of which 75 cubic meters of photosynthetic bacteria liquid, 50 cubic meters of single-cell algae liquid, and fresh branches were produced. 355 kg of horns, about 310 cubic meters of discharge water, the main water quality indicators of discharge water are as follows: chemical oxygen consumption<300mg/L, ammonia nitrogen<50mg/L, total phosphorus<5mg/L, pH6.8-7.5, Transparency > 40cm.
一种猪场沼液资源化利用的方法与传统方法相比具有明显优点,本发明的猪场沼液资源化利用方法可将沼液处理过程中生成的光合细菌、单细胞藻类和枝角类用于水产养殖和饵料,借助食物链将沼液中的污染物质实现资源化利用,具有投入低、能耗低、可产生明显的经济和生态效益的特点;传统的废水治理工程只能削减污染物的排放量与排放强度,通常少有产品产出,只能产生环境、生态和社会效益,难以产生经济效益。这种只投入无产出的模式导致企业对环保投入不积极,而本发明将污水处理与活饵料生产耦合起来,不但能实现污水有效处理,且能降低活饵料生产的成本,实现环境的可持续及资源的循环利用,符合国家发展生态农业、绿色农业和循环农业的趋势;Compared with traditional methods, a method for resource utilization of pig farm biogas slurry has obvious advantages. The method for resource utilization of pig farm biogas slurry can use photosynthetic bacteria, unicellular algae and cladocerans generated in the process of biogas slurry treatment For aquaculture and bait, the pollutants in the biogas slurry can be utilized as resources by means of the food chain, which has the characteristics of low investment, low energy consumption, and can produce obvious economic and ecological benefits; traditional wastewater treatment projects can only reduce the amount of pollutants Emissions and emission intensity usually have little product output, which can only produce environmental, ecological and social benefits, and it is difficult to produce economic benefits. This mode of only investing but not producing results in enterprises being inactive in environmental protection investment, and the present invention couples sewage treatment with live bait production, not only can realize effective treatment of sewage, but also can reduce the cost of live bait production and realize environmental sustainability and resource recycling, in line with the national trend of developing ecological agriculture, green agriculture and circular agriculture;
畜禽养殖污水既是严重的污染源,也是宝贵的资源。本发明在传统畜禽养殖污水处理工艺中引入光合细菌、微藻和枝角类,通过光合细菌-微藻-枝角类食物链将猪场沼液净化及活饵料生产相耦合,利用猪场沼液中高浓度的氮、磷、有机物等营养盐培养光合细菌和微藻,将所培养光合细菌和微藻通过枝角类滤食生产活饵料用于动物养殖生产,从而实现环境保护及资源的高效循环利用,保障生产和生态的可持续发展。Livestock and poultry breeding sewage is not only a serious source of pollution, but also a valuable resource. The invention introduces photosynthetic bacteria, microalgae and cladocera into the traditional livestock and poultry breeding sewage treatment process, and couples the pig farm biogas slurry purification and live bait production through the photosynthetic bacteria-microalgae-cladocera food chain, and utilizes the pig farm biogas slurry Medium and high concentrations of nitrogen, phosphorus, organic matter and other nutrient salts are used to cultivate photosynthetic bacteria and microalgae, and the cultured photosynthetic bacteria and microalgae are used to produce live bait through Cladocera filter feeding for animal breeding and production, thereby realizing environmental protection and efficient recycling of resources Use to ensure the sustainable development of production and ecology.
以上所述仅是本发明的优选实施方式,应当指出,对于本技术领域的普通技术人员,在不脱离本发明方法的前提下,还可以做出若干改进和补充,这些改进和补充也应视为本发明的保护范围。The above is only a preferred embodiment of the present invention, it should be pointed out that for those of ordinary skill in the art, without departing from the method of the present invention, some improvements and supplements can also be made, and these improvements and supplements should also be considered Be the protection scope of the present invention.
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CN104542423A (en) * | 2015-02-05 | 2015-04-29 | 朱祖阳 | Cellular totally-closed recirculating aquaculture system device |
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CN101074144A (en) * | 2006-05-17 | 2007-11-21 | 郭文瑞 | Ecological treatment and recovering utilization for farm |
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CN102267787A (en) * | 2011-07-27 | 2011-12-07 | 福建科佳奇迈生物工程有限公司 | Method for treating livestock and poultry culture polluted marsh solution |
CN104450513A (en) * | 2013-11-26 | 2015-03-25 | 朱祖阳 | Full-automatic factory full-wave band closed circulating water real-time monitoring breeding device |
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CN106277320A (en) * | 2016-08-31 | 2017-01-04 | 中国科学院汕头海洋植物实验站 | A kind of Penaeus vannamei freshwater cultivation water regulation method |
CN106277320B (en) * | 2016-08-31 | 2019-10-29 | 中国科学院汕头海洋植物实验站 | A kind of Penaeus Vannmei freshwater cultivation water regulation method |
CN107226596A (en) * | 2017-08-10 | 2017-10-03 | 成都钧乔农业科技开发有限公司 | A kind of technique for being engineered bacterium algae fermentation herding liquid dung and Collocation cultivation water |
CN107226596B (en) * | 2017-08-10 | 2020-08-11 | 成都钧乔农业科技开发有限公司 | Process for fermenting livestock manure water by engineering bacteria and algae and matched culture water |
CN107509673B (en) * | 2017-08-18 | 2020-08-18 | 上海海洋大学 | A kind of resource utilization method of intensive prawn aquaculture wastewater |
CN109090363A (en) * | 2018-09-04 | 2018-12-28 | 肖树恩 | A kind of feed and preparation method and ecological circulation agricultural methods |
CN110128181A (en) * | 2019-06-14 | 2019-08-16 | 武汉益锦祥生物环保有限公司 | A kind of production method and process units of the phycomycete fertilizer based on biogas slurry |
CN114716110A (en) * | 2022-04-27 | 2022-07-08 | 广州市环境保护工程设计院有限公司 | Aquaculture wastewater treatment system and method |
CN115072879A (en) * | 2022-08-23 | 2022-09-20 | 河北雄安德荫源环境科技有限公司 | System and method for biological purification and resource conversion and utilization of sewage |
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