[go: up one dir, main page]

CN105506055A - Selective medium for listeria monocytogenes of stool sample - Google Patents

Selective medium for listeria monocytogenes of stool sample Download PDF

Info

Publication number
CN105506055A
CN105506055A CN201610079558.2A CN201610079558A CN105506055A CN 105506055 A CN105506055 A CN 105506055A CN 201610079558 A CN201610079558 A CN 201610079558A CN 105506055 A CN105506055 A CN 105506055A
Authority
CN
China
Prior art keywords
listeria monocytogenes
sterilizing
agar
sodium
selective medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201610079558.2A
Other languages
Chinese (zh)
Inventor
夏淑平
唐志勐
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201610079558.2A priority Critical patent/CN105506055A/en
Publication of CN105506055A publication Critical patent/CN105506055A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/045—Culture media therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention discloses a selective medium for listeria monocytogenes of a stool sample, and belongs to the field of inspection microbial culture. The selective medium is characterized in that the formula of each 1,000 ml of the selective medium contains yeast extract powder, beef powder, casein peptone, agar, glucose, sodium chloride, zinc gluconate, sodium dihydrogen phosphate, glutathione, polymyxin, bacitracin and sterile horse blood protein, and the balance of distilled water is added into the mixture. Compared with the prior art, the selective medium has the characteristics of being reliable in inspection, and high in separation rate of the listeria monocytogenes.

Description

A kind of Selective agar medium for stool sample Listeria monocytogenes
Technical field
The present invention relates to inspection microorganism culturing field, be specifically related to a kind of Selective agar medium for stool sample Listeria monocytogenes.
Background technology
Microorganism culturing is a kind of technology making microbial growth by artificial means, and the substratum that selective medium is used to promote or suppress the organism (as cell or bacterium etc.) of certain type and designs, utilize this substratum required microorganism can be separated from the microorganism mixed.Listeria monocytogenes (Listeriamonocytogenes) is a kind of facultative anaerobic bacteria, extensively be distributed in physical environment, as soil, sewage, feed, animal, Healthy People and variously eat raw with in instant food, comprise raw meat and cooked meat product, quick-frozen rice and flour food, cheese, vegetables, salad and sea-food etc., mankind's listeriosis can be caused, normally cause because people eat by the food of this fungi pollution.After infecting, main clinical manifestation is heating, upper respiratory tract infection, pneumonia, diarrhoea, bloodstream infection, meningitis, or infects foetus via pregnant woman's private parts, cause meningitis, even dead.Similar to influenza in clinical symptom, easy mistaken diagnosis.
In recent years, listeriosis is unrare, and all can occur in the crowd of newborn infant, gravid woman, more than 40 years old and immune function depression, especially gravid woman infects 30 ~ 100 times that listerial risk is general population.Early diagnosis early treatment, can 100% to cure.If delay in diagnosis, case fatality rate can reach 20 ~ 30%, and the correct and Rapid identification therefore as the clinical bacteria inspection of making a definite diagnosis unique foundation is very important.At present, this Pseudomonas identify gold standard be PCR qualification, but PCR test set require and running cost all higher, the therefore multiplex trypticase soy agar (TSA-YE) of clinical cultivation.But it is low that Listeria monocytogenes cultivates positive rate, mainly in clinical culturing process, this bacterium is easily considered to the miscellaneous bacteria (diphtheroid) of pollution and is abandoned, and the intestinal bacteria, diphtheroid etc. in stool sample are faster than Listeria monocytogenes growth on current substratum, cover substratum very soon, Listeria monocytogenes cannot grow, and result incurs loss through delay consultation hours.
Summary of the invention
Technical assignment of the present invention is for above the deficiencies in the prior art, provides a kind of Selective agar medium being suitable for stool sample Listeria monocytogenes.
The technical scheme that the present invention solves its technical problem is: a kind of Selective agar medium being suitable for stool sample Listeria monocytogenes, is characterized by, and contains in the formula of preparation 1000ml substratum:
Yeast extract powder 5 ~ 8g;
Beef powder 6 ~ 10g;
Casein peptone 6 ~ 10g;
Agar 15 ~ 20g;
Glucose 3 ~ 5g;
Sodium-chlor 5 ~ 5.5g;
Zinc Gluconate 0.01 ~ 0.05g;
SODIUM PHOSPHATE, MONOBASIC 1 ~ 3g;
The sweet peptide 0.1 ~ 0.5g of optical valley;
Polymyxin 0.001 ~ 0.005g;
Bacitracin 0.001 ~ 0.003g;
Aseptic horse haemproteins 20 ~ 50g;
Distilled water adds to 1000ml.
The preparation method of above-mentioned substratum is: take yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, SODIUM PHOSPHATE, MONOBASIC, and mixing distilled water dissolves, and 210 DEG C, sterilizing 15min, is cooled to 45 DEG C, adds; Sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Wherein: described beef powder, casein peptone, glucose provide carbon source, nitrogenous source, the nutritive ingredients such as yeast extract powder rich in proteins, amino acid, polypeptide, Nucleotide, VITAMIN, somatomedin, trace element, the Growth and Reproduction for Listeria monocytogenes provides balanced nutritive element; Sodium-chlor maintains balanced osmotic pressure; Agar is the peptizer of substratum; Zinc Gluconate is nutrition agent, and contriver finds, add certain density zine ion in the medium, the growth conditions of object bacteria is better than control group, and therefore inference zine ion contributes to the growth of this bacterium; SODIUM PHOSPHATE, MONOBASIC is buffer reagent, and polymyxin suppresses the growth of Gram-negative bacteria; Bacitracin (Bacitracin), laboratory study shows that bacitracin can suppress the growth of the miscellaneous bacteria such as gram-positive bacteria and negative cocci, pneumococcus, staphylococcus, gonococcus, meningococcus, but under this concentration due to listeria without antibacterial and bacteriostasis, therefore improve the separation rate of Listeria monocytogenes.By experiment same Listeria monocytogenes is inoculated on 2 TSA-YE substratum, one of them TSA-YE substratum add the sweet peptide of 0.01% sterilizing optical valley in, cultivate at 37 DEG C, bacterial strain is observed after 48 hours, find that the TSA-YE substratum adding the sweet peptide of optical valley has little Listeria monocytogenes bacterium colony, the TSA-YE substratum not adding the sweet peptide of optical valley does not find Listeria monocytogenes bacterium colony, and the sweet peptide of optical valley can reduce redox-potential, is conducive to Growth of Cells, differentiation, aseptic horse haemproteins provides energy environment, γ-hydroxyarginine is activator, can promote that Listeria monocytogenes is to the picked-up of mineral substance, apiolin is fungistat, Fructus Corni is the fruit of Cornaceae plant skunk bush, modern medicine study shows, Fructus Corni to be tanned hair containing skunk bush, skunk bush splits glucoside, iron, aluminium, copper, zinc, boron, 21 kinds of elements such as phosphorus, Threonine, α-amino-isovaleric acid, leucine etc. 14 seed amino acid, vitamin A, glucose, fructose, sucrose, ursolic acid, gallic acid, oxysuccinic acid, the carbohydrates such as tartrate and organic acid, Fructus Corni Aqueous extracts is good nutrition-fortifying agent, and to streptococcus aureus, Nai Shi micrococcus catarrhalis, tubercule bacillus, pneumococcus, hemophilus influenzae, polioviruses etc. all have bacteriostatic action in various degree.
The present invention compared with prior art, has and detects the feature reliable, Listeria monocytogenes separation rate is high.
Embodiment
Below in conjunction with practical situation, the specific embodiment of the present invention is elaborated.
Embodiment 1, contains in the formula of preparation 1000ml substratum: yeast extract powder 5g; Beef powder 10g; Casein peptone 6g; Agar 15g; Glucose 4g; Sodium-chlor 5g; SODIUM PHOSPHATE, MONOBASIC 1g; The sweet peptide 0.3g of optical valley; Zinc Gluconate 0.01g; Polymyxin 0.001g; Bacitracin 0.003g; Aseptic horse haemproteins 20g, distilled water adds to 1000ml.Preparation method: take yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, SODIUM PHOSPHATE, MONOBASIC, mixing distilled water dissolves, and 210 DEG C, sterilizing 15min, is cooled to 45 DEG C, adds sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Embodiment 2, contains in the formula of preparation 1000ml substratum: yeast extract powder 5g; Beef powder 6g; Casein peptone 8g; Agar 15g; Glucose 3g; Sodium-chlor 5g; SODIUM PHOSPHATE, MONOBASIC 3g; The sweet peptide 0.1g of optical valley; Zinc Gluconate 0.05g; Polymyxin 0.003g; Bacitracin 0.001g; γ-hydroxyarginine 0.1g; Aseptic horse haemproteins 40g, distilled water adds to 1000ml.Preparation method: take yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, γ-hydroxyarginine, SODIUM PHOSPHATE, MONOBASIC, mixing distilled water dissolves, and 210 DEG C, sterilizing 15min, is cooled to 45 DEG C, adds sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Embodiment 3, contains in the formula of preparation 1000ml substratum: yeast extract powder 5g; Beef powder 8g; Casein peptone 6g; Agar 18g; Glucose 5g; Sodium-chlor 5.5g; SODIUM PHOSPHATE, MONOBASIC 2g; The sweet peptide 0.5g of optical valley; Zinc Gluconate 0.03g; Polymyxin 0.005g; Bacitracin 0.001g; Apiolin 0.03g; Aseptic horse haemproteins 50g, distilled water adds to 1000ml.Preparation method: take yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, SODIUM PHOSPHATE, MONOBASIC, apiolin, mixing distilled water dissolves, and 210 DEG C, sterilizing 15min, is cooled to 45 DEG C, adds sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Embodiment 4, contains in the formula of preparation 1000ml substratum: yeast extract powder 6g; Beef powder 10g; Casein peptone 8g; Agar 18g; Glucose 4g; Sodium-chlor 5g; SODIUM PHOSPHATE, MONOBASIC 2g; The sweet peptide 0.1g of optical valley; Zinc Gluconate 0.03g; Polymyxin 0.005g; Bacitracin 0.002g; Aseptic horse haemproteins 30g; 200g/L Fructus Corni Aqueous extracts 200ml; Distilled water adds to 1000ml.Preparation method: (1) is got 40g Fructus Corni and added water boil 30min, filters, gets filtrate, according to filtrate volume, taking the concentrated or adjustment volume that adds water to be 200g/L to concentration, obtaining Fructus Corni Aqueous extracts 200ml; (2) take yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, SODIUM PHOSPHATE, MONOBASIC, be dissolved in the Fructus Corni extracting solution of step (1) gained and mix, 210 DEG C of sterilizing 15min, are cooled to 45 DEG C, add sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Embodiment 5, contains in the formula of preparation 1000ml substratum: yeast extract powder 6g; Beef powder 6g; Casein peptone 8g; Agar 20g; Glucose 3g; Sodium-chlor 5.5g; SODIUM PHOSPHATE, MONOBASIC 3g; The sweet peptide 0.5g of optical valley; Zinc Gluconate 0.01g; Polymyxin 0.001g; Bacitracin 0.003g; γ-hydroxyarginine 0.2g; Apiolin 0.03g; Aseptic horse haemproteins 40g, distilled water adds to 1000ml.Preparation method: take yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, SODIUM PHOSPHATE, MONOBASIC, γ-hydroxyarginine, apiolin, mixing distilled water dissolves, 210 DEG C, sterilizing 15min, be cooled to 45 DEG C, add sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Embodiment 6, contains in the formula of preparation 1000ml substratum: yeast extract powder 8g; Beef powder 8g; Casein peptone 10g; Agar 20g; Glucose 3g; Sodium-chlor 5.5g; SODIUM PHOSPHATE, MONOBASIC 3g; The sweet peptide 0.5g of optical valley; Zinc Gluconate 0.01g; Polymyxin 0.001g; Bacitracin 0.003g; γ-hydroxyarginine 0.3g; Aseptic horse haemproteins 50g; The Fructus Corni Aqueous extracts 250ml of concentration 200g/L; Distilled water adds to 1000ml.Preparation method: (1) is got 50g Fructus Corni and added water boil 45min, filters, gets filtrate, according to filtrate volume, taking the concentrated or adjustment volume that adds water to be 200g/L to concentration, obtaining Fructus Corni Aqueous extracts 250ml; (2) yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, SODIUM PHOSPHATE, MONOBASIC, γ-hydroxyarginine is taken, be dissolved in the Fructus Corni extracting solution of step (1) gained and mix, 210 DEG C of sterilizing 15min, are cooled to 45 DEG C, add sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Embodiment 7, contains in the formula of preparation 1000ml substratum: yeast extract powder 8g; Beef powder 8g; Casein peptone 10g; Agar 15g; Glucose 4g; Sodium-chlor 5g; SODIUM PHOSPHATE, MONOBASIC 3g; The sweet peptide 0.3g of optical valley; Zinc Gluconate 0.03g; Polymyxin 0.003g; Bacitracin 0.001g; Apiolin 0.01g; Aseptic horse haemproteins 20g; The Fructus Corni Aqueous extracts 300ml of concentration 200g/L; Distilled water adds to 1000ml.Preparation method: (1) is got 60g Fructus Corni and added water boil 60min, filters, gets filtrate, according to filtrate volume, taking the concentrated or adjustment volume that adds water to be 200g/L to concentration, obtaining Fructus Corni Aqueous extracts 300ml; (2) yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, SODIUM PHOSPHATE, MONOBASIC, apiolin is taken, be dissolved in the Fructus Corni extracting solution of step (1) gained and mix, 210 DEG C of sterilizing 15min, are cooled to 45 DEG C, add sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Embodiment 8, contains in the formula of preparation 1000ml substratum: yeast extract powder 8g; Beef powder 10g; Casein peptone 6g; Agar 18g; Glucose 3g; Sodium-chlor 5.5g; SODIUM PHOSPHATE, MONOBASIC 2g; The sweet peptide 0.3g of optical valley; Zinc Gluconate 0.01g; Polymyxin 0.005g; Bacitracin 0.001g; γ-hydroxyarginine 0.3g; Apiolin 0.03g; Aseptic horse haemproteins 30g; The Fructus Corni Aqueous extracts 250ml of concentration 200g/L; Distilled water adds to 1000ml.Preparation method: (1) is got 60g Fructus Corni and added water boil 60min, filters, gets filtrate, according to filtrate volume, taking the concentrated or adjustment volume that adds water to be 200g/L to concentration, obtaining Fructus Corni Aqueous extracts 300ml; (2) yeast extract powder, beef powder, casein peptone, agar, glucose, sodium-chlor, γ-hydroxyarginine, SODIUM PHOSPHATE, MONOBASIC, apiolin is taken, be dissolved in the Fructus Corni extracting solution of step (1) gained and mix, 210 DEG C of sterilizing 15min, are cooled to 45 DEG C, add sterile dextrose acid zinc; The sweet peptide of sterilizing optical valley; Sterilizing polymyxin; Sterilizing bacitracin; Aseptic horse haemproteins mixing, packing after sterile purified water constant volume to 1000ml.
Described Fructus Corni Aqueous extracts concentration 200g/L refers to often liter containing crude drug 200g.
Gained Listeria monocytogenes Selective agar medium of the present invention is used for stool sample, and have and detect the feature reliable, Listeria monocytogenes separation rate is high, be clinical data sufficient proof, pertinent data is as follows.
1 object and method.
1.1 medium preparing: experimental group establishes 4 groups altogether, are respectively embodiment 1 scheme group; Embodiment 2 scheme group, embodiment 6 scheme group, embodiment 8 scheme group, control group is trypticase soy agar (TSA-YE) substratum, and its formula is: containing peptone 17.0g in often liter; Multivalence peptone 3.0g; Sodium-chlor 5.0g; Dipotassium hydrogen phosphate 2.5g; Glucose 2.5g; Agar 15.0.
1.2 fecal sample process, cultural method: outpatient service or 156 examples of being in hospital are suffered from diarrhoea and with similar flu-like symptom patient fresh excreta, are suspended in 5ml0.9% physiological saline, are inoculated in embodiment 1 scheme group with transfering loop sectional streak; Embodiment 2 scheme group; Embodiment 6 scheme group; Embodiment 8 scheme group and control group substratum.Heat insulating culture 48h under the substratum inoculated 35 DEG C of atmospheric condition, the tiny bacterium colony of picking carries out identification of bacteria.Each sample number censorship PCR does molecular biology identification.
1.3 detect positive criteria: take out culture dish, carry out object bacteria selection and qualification: observe colony growth situation, select object bacteria smear, gramstaining, sediments microscope inspection according to " clinical microbiology diagnosis and diagram ".Judge that net result gold standard is fluorescent quantitative PCR result.
2 results: what 156 parts of diarrhea patient stool samples detected that bacterium colony is finally accredited as the Listeria monocytogenes positive through PCR is 19 examples.Each group of 48h detects the positive, false positive, feminine gender, false negative bacterial strain number of cases see the following form,
The above results can be found out, cultivate after 48 hours, each embodiment group and control group positive rate more all have notable difference (P<0.05), and the false negative rate of each embodiment group is all lower than control group, have pole notable difference (P<0.001).The control group positive detects result and compares with PCR detected result, the huge (χ of positive rate difference 2=7.35, P<0.01), and each embodiment group compares with PCR detected result, positive rate no significant difference (P>0.05).
Above result shows, stool sample is after embodiment of the present invention culture medium culturing, Listeria monocytogenes positive rate and PCR checkout discrepancy no difference of science of statistics, and separation rate is apparently higher than existing substratum, general T SA-YE substratum selectivity is not enough, in ight soil, miscellaneous bacteria ramp covers the Growth positive that substratum affects Listeria monocytogenes, substratum of the present invention can be tried one's best bacterial strain positive rate accurately, Listeria monocytogenes detects reliably, positive separation rate is high, thus the time is made a definite diagnosis in shortening.

Claims (1)

1., for a Selective agar medium for stool sample Listeria monocytogenes, it is characterized in that containing in the formula of preparation 1000ml substratum:
Yeast extract powder 5 ~ 8g;
Beef powder 6 ~ 10g;
Casein peptone 6 ~ 10g;
Agar 15 ~ 20g;
Glucose 3 ~ 5g;
Sodium-chlor 5 ~ 5.5g;
Zinc Gluconate 0.01 ~ 0.05g;
SODIUM PHOSPHATE, MONOBASIC 1 ~ 3g;
The sweet peptide 0.1 ~ 0.5g of optical valley;
Polymyxin 0.001 ~ 0.005g;
Bacitracin 0.001 ~ 0.003g;
Aseptic horse haemproteins 20 ~ 50g;
Distilled water adds to 1000ml.
CN201610079558.2A 2016-02-05 2016-02-05 Selective medium for listeria monocytogenes of stool sample Pending CN105506055A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610079558.2A CN105506055A (en) 2016-02-05 2016-02-05 Selective medium for listeria monocytogenes of stool sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610079558.2A CN105506055A (en) 2016-02-05 2016-02-05 Selective medium for listeria monocytogenes of stool sample

Publications (1)

Publication Number Publication Date
CN105506055A true CN105506055A (en) 2016-04-20

Family

ID=55714345

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610079558.2A Pending CN105506055A (en) 2016-02-05 2016-02-05 Selective medium for listeria monocytogenes of stool sample

Country Status (1)

Country Link
CN (1) CN105506055A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112384631A (en) * 2018-06-20 2021-02-19 3M创新有限公司 Enrichment supplement for antimicrobial matrices

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5296370A (en) * 1990-10-04 1994-03-22 Rutgers, The State University Repair medium for the resuscitation of injured cells
WO2007061848A2 (en) * 2005-11-17 2007-05-31 The Trustees Of The University Of Pennsylvania Methods for producing, growing, and preserving listeria vaccine vectors
US20070259393A1 (en) * 2006-05-02 2007-11-08 Lawrence Restaino Plating media for the presumptive identification of Listeria sp, Listeria monocytogenes and Listeria ivanovii
CN101412978A (en) * 2008-11-25 2009-04-22 华南理工大学 Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof
US8501457B2 (en) * 2010-08-30 2013-08-06 Samsung Techwin Co., Ltd. Enrichment of Listeria spp
CN104195214A (en) * 2014-09-12 2014-12-10 青岛润鑫伟业科贸有限公司 Listeriosis chromogenic medium

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5296370A (en) * 1990-10-04 1994-03-22 Rutgers, The State University Repair medium for the resuscitation of injured cells
WO2007061848A2 (en) * 2005-11-17 2007-05-31 The Trustees Of The University Of Pennsylvania Methods for producing, growing, and preserving listeria vaccine vectors
US20070259393A1 (en) * 2006-05-02 2007-11-08 Lawrence Restaino Plating media for the presumptive identification of Listeria sp, Listeria monocytogenes and Listeria ivanovii
CN101412978A (en) * 2008-11-25 2009-04-22 华南理工大学 Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof
US8501457B2 (en) * 2010-08-30 2013-08-06 Samsung Techwin Co., Ltd. Enrichment of Listeria spp
CN104195214A (en) * 2014-09-12 2014-12-10 青岛润鑫伟业科贸有限公司 Listeriosis chromogenic medium

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
中华人民共和国卫生部: "食品安全国家标准，单核增生李斯特氏菌检验", 《GB 4789.30-2010》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112384631A (en) * 2018-06-20 2021-02-19 3M创新有限公司 Enrichment supplement for antimicrobial matrices
US20210277352A1 (en) * 2018-06-20 2021-09-09 3M Innovative Properties Company Enrichment supplement for antimicrobal matrices

Similar Documents

Publication Publication Date Title
CN101812414B (en) Lactobacillus plantarum and bacteriocins produced by lactobacillus plantarum and capable of inhibiting Gram negative bacteria
CN103013879B (en) Bacterial strain having capacity of giving high yield of Gamma-aminobutyric acid
CN103478509B (en) Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed
CN105175518B (en) The bacteriocin and preparation method thereof that bacillus coagulans FM603 is generated
CN105176874B (en) Bacillus coagulans FM603 and its application
CN113249259B (en) A kind of Bacillus subtilis with the functions of inhibiting bacteria and reducing the hemagglutination value of virus and its application
CN116515717B (en) Lactobacillus paracasei BIGTREE-B101145 and application thereof
CN105104712B (en) A kind of additive for microbe feedstuff and preparation method thereof
CN110484467A (en) Antibacterial peptide and the application of one plant of bacillus polymyxa and its generation
CN113403225B (en) Bifidobacterium animalis NSY0201 and application thereof in preparation of health care products or medicines for enhancing immunity of organisms
CN112410252B (en) A Plutella xylostella strain PxCG2 and its application
CN104072593A (en) Hericium erinaceus glycoprotein with anti-tumor and agglutination activity and preparation method thereof
CN105506055A (en) Selective medium for listeria monocytogenes of stool sample
CN108410767A (en) Bacillus natto and its application in fermentation Ruditapes philippinarum prepares active polysaccharide
CN105506054A (en) Listeria monocytogenes selective medium
CN118109353A (en) A Pediococcus acidilactici strain and its application
CN110452849A (en) A kind of probiotic lactobacillus plantarum
CN105543326A (en) Listerella selective culture medium
CN104845917A (en) Enrichment culture medium
CN112094777B (en) Lactobacillus plantarum and application thereof in Laoshan milk goat feed
CN104878070A (en) Anaerobic culture medium for culturing fusobacterium necrophorum
CN105506056A (en) Feces sample listeria monocytogenes culture medium
CN104846058A (en) Nocardia asteroid selective medium for sputum specimen
CN104830955A (en) Nocardia asteroid selective medium for sputum samples
CN117247859B (en) A sheep-derived Lactococcus garvieae and a probiotic agent thereof for combating Clostridium perfringens

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20160420

RJ01 Rejection of invention patent application after publication