CN105400840A - Inducer acting on metabolic regulation of Chinese yew cells - Google Patents
Inducer acting on metabolic regulation of Chinese yew cells Download PDFInfo
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- CN105400840A CN105400840A CN201510968250.9A CN201510968250A CN105400840A CN 105400840 A CN105400840 A CN 105400840A CN 201510968250 A CN201510968250 A CN 201510968250A CN 105400840 A CN105400840 A CN 105400840A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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Abstract
The invention provides an inducer acting on metabolic regulation of Chinese yew cells. The inducer comprises sodium citrate, gibberellin, albumin, luteolin, silkworm pupae powder, yeast powder, dried orange peel, inula flowers, caulis clematidis armandii, cortex periplocae and other components. The metabolic mode of the Chinese yew cells is interfered by adding elicitors in a culture medium, so that the Chinese yew cells secrete a docetaxel semi-synthesis precursor of 10-diacetylbaccatin III in an orient induction mode. Experiments show that after the inducer is added into cell culture fluid, the amount of 10-diacetylbaccatin III secreted by the Chinese yew cells is remarkably increased, and meanwhile the cell culture density can be increased to a certain extent. The technological concept is clear, the technical scheme is reasonable, and the inducer has good large-scale application prospects.
Description
Technical field
The present invention relates to cell engineering field, be specifically related to a kind of inductor acting on yew cell metabolic regulation.
Background technology
Taxol (paclitaxel) extracts the diterpene-kind compound obtained from the bark of taxaceae Taxus (Taxus) plant.It is a kind of novel microtubule stabilizer, has unique antitumour activity, is thought the most important progress of chemotherapy of tumors over nearly 15 ~ 20 years by national cancer institute.As the second line treatment medicine of advanced ovarian cancer, get permission listing in more than 40 countries so far, and show challenging curative effect in the treatment such as vascular restenosis of mammary cancer, lung cancer, leukemia, gastrointestinal cancer and PTCA or and STENTS.Taxol limits its clinical application due to scarcity of resources and water-soluble low problem.
Docetaxel is the taxane-ring structure containing a band aerobic Fourth Ring on C4 and C5 position, and containing a huge ester side chain on C13 position.Show in its external activity structural relation the characteristic of C13 ester side chain and configuration for Docetaxel In Vitro Anti tubulin activity put close important.For taxol, the water-soluble remarkable lifting of Docetaxel, for the pharmaceutical activity of apoptosis and cancer therapy higher than taxol, therefore its preparation method receives and pays close attention to widely.
In prior art, the preparation method of Docetaxel utilizes its precursor substance 10-deacetylbaccatin III to carry out chemically modified realization, and this precursor substance still obtains mainly through the mode of Taxus chinensis cell cultures at present.But on the one hand due to the restriction by metabolic regulation, the tendency of yew cell secretion 10-deacetylbaccatin III is not vigorous; Be limited to the factor such as culture density, cell yield on the other hand, make the yield of target product lower.
Summary of the invention
The present invention is intended to the technological deficiency for prior art, provides a kind of inductor acting on yew cell metabolic regulation, to solve the technical problem that in prior art, the secretory volume of yew cell to 10-deacetylbaccatin III is lower.
For realizing above technical purpose, the present invention by the following technical solutions:
A kind of inductor acting on yew cell metabolic regulation, comprise the composition of following weight part: Trisodium Citrate 8 ~ 10 parts, Plant hormones regulators,gibberellins 4 ~ 6 parts, albumin 2 ~ 3 parts, luteolin 1 ~ 2 part, dried silkworm chrysalis meal 1 ~ 2 part, yeast powder 1 ~ 2 part, dried orange peel 1 ~ 2 part, Flos Inulae 1 ~ 2 part, Stem of Armand Clematis 1 ~ 2 part, Root-bark of Chinese Silkvine 1 ~ 2 part, 50 ~ 60 parts, water.
As preferably, also comprise the Leaf of Indigowoad of 1 ~ 2 weight part.
As preferably, also comprise the rhamnosyl of 1 ~ 2 weight part.
As preferably, also comprise the N.F,USP MANNITOL of 1 ~ 2 weight part.
As preferably, also comprise the Wrinkled Gianthyssop Herb of 1 ~ 2 weight part.
As preferably, described inductor is grouped into by the one-tenth of following weight part: Trisodium Citrate 9 parts, Plant hormones regulators,gibberellins 5 parts, albumin 2.5 parts, luteolin 1.5 parts, dried silkworm chrysalis meal 1.5 parts, yeast powder 1.5 parts, dried orange peel 1.5 parts, Flos Inulae 1.5 parts, Stem of Armand Clematis 1.5 parts, Root-bark of Chinese Silkvine 1.5 parts, 55 parts, water.
As preferably, described inductor is grouped into by the one-tenth of following weight part: Trisodium Citrate 8.5 parts, Plant hormones regulators,gibberellins 5.5 parts, albumin 2.1 parts, luteolin 1.3 parts, dried silkworm chrysalis meal 1.7 parts, yeast powder 1.6 parts, dried orange peel 1.2 parts, Flos Inulae 1.1 parts, Stem of Armand Clematis 1.4 parts, Root-bark of Chinese Silkvine 1.5 parts, Leaf of Indigowoad 1.4 parts, rhamnosyl 1.1 parts, 1.5 parts, N.F,USP MANNITOL, Wrinkled Gianthyssop Herb 1.8 parts, 57 parts, water.
The present invention interferes yew cell own metabolism pattern by the mode of adding elicitor in the medium, thus the semi-synthetic precursor of directional induction yew cell secretion Docetaxel---10-deacetylbaccatin III.The present invention's preferred various trace elements determine optimum proportioning on the usual nutrition basis for cell cultures, with the addition of the productive rate that a certain amount of natural medicinal ingredients is intended to promote further this precursor substance on this basis.Experiment finds, add inductor of the present invention in cell culture fluid after, the secretory volume of yew cell to 10-deacetylbaccatin III significantly promotes, and to a certain degree can promote cell culture density simultaneously.The technology of the present invention clear thinking, technical scheme is reasonable, has good mass-producing application prospect.
Embodiment
Below will be described in detail the specific embodiment of the present invention.In order to avoid too much unnecessary details, in the examples below to belonging to known structure or function will not be described in detail.Apart from outside definition, technology used in following examples and scientific terminology have the identical meanings generally understood with those skilled in the art of the invention.
Embodiment 1
Act on an inductor for yew cell metabolic regulation, be grouped into by the one-tenth of following weight part: Trisodium Citrate 8 parts, Plant hormones regulators,gibberellins 4 parts, albumin 2 parts, luteolin 1 part, dried silkworm chrysalis meal 1 part, yeast powder 1 part, dried orange peel 1 part, Flos Inulae 1 part, Stem of Armand Clematis 1 part, Root-bark of Chinese Silkvine 1 part, 50 parts, water.
Embodiment 2
Act on an inductor for yew cell metabolic regulation, be grouped into by the one-tenth of following weight part: Trisodium Citrate 10 parts, Plant hormones regulators,gibberellins 6 parts, albumin 3 parts, luteolin 2 parts, dried silkworm chrysalis meal 2 parts, yeast powder 2 parts, dried orange peel 2 parts, Flos Inulae 2 parts, Stem of Armand Clematis 2 parts, Root-bark of Chinese Silkvine 2 parts, 60 parts, water.
Embodiment 3
A kind of inductor acting on yew cell metabolic regulation, be grouped into by the one-tenth of following weight part: Trisodium Citrate 9 parts, Plant hormones regulators,gibberellins 5 parts, albumin 2.3 parts, luteolin 1.8 parts, dried silkworm chrysalis meal 1.5 parts, yeast powder 1.6 parts, dried orange peel 1.7 parts, Flos Inulae 1.8 parts, Stem of Armand Clematis 1.2 parts, Root-bark of Chinese Silkvine 1.8 parts, 55 parts, water.
Embodiment 4
Act on an inductor for yew cell metabolic regulation, be grouped into by the one-tenth of following weight part: Trisodium Citrate 8.8 parts, Plant hormones regulators,gibberellins 5.4 parts, albumin 2.7 parts, luteolin 1.2 parts, dried silkworm chrysalis meal 1.2 parts, yeast powder 1.2 parts, dried orange peel 1.5 parts, Flos Inulae 1.6 parts, Stem of Armand Clematis 1.4 parts, Root-bark of Chinese Silkvine 1.7 parts, Leaf of Indigowoad 1 part, Wrinkled Gianthyssop Herb 1.1 parts, 52 parts, water.
Embodiment 5
Act on an inductor for yew cell metabolic regulation, be grouped into by the one-tenth of following weight part: Trisodium Citrate 9.4 parts, Plant hormones regulators,gibberellins 4.2 parts, albumin 2.1 parts, luteolin 1.3 parts, dried silkworm chrysalis meal 1.1 parts, yeast powder 1 part, dried orange peel 2 parts, Flos Inulae 1.9 parts, Stem of Armand Clematis 1.2 parts, Root-bark of Chinese Silkvine 1.9 parts, rhamnosyl 1.3 parts, 1.2 parts, N.F,USP MANNITOL, Wrinkled Gianthyssop Herb 1.6 parts, 58 parts, water.
Embodiment 6
Act on an inductor for yew cell metabolic regulation, be grouped into by the one-tenth of following weight part: Trisodium Citrate 8.8 parts, Plant hormones regulators,gibberellins 5.4 parts, albumin 2.7 parts, luteolin 1.2 parts, dried silkworm chrysalis meal 1.2 parts, yeast powder 1.2 parts, dried orange peel 1.5 parts, Flos Inulae 1.6 parts, Stem of Armand Clematis 1.4 parts, Root-bark of Chinese Silkvine 1.7 parts, Leaf of Indigowoad 1.5 parts, 55 parts, water.
Embodiment 7
Act on an inductor for yew cell metabolic regulation, be grouped into by the one-tenth of following weight part: Trisodium Citrate 9.2 parts, Plant hormones regulators,gibberellins 5.7 parts, albumin 2.4 parts, luteolin 1.5 parts, dried silkworm chrysalis meal 1.5 parts, yeast powder 1.7 parts, dried orange peel 1.7 parts, Flos Inulae 1.2 parts, Stem of Armand Clematis 1.9 parts, Root-bark of Chinese Silkvine 2 parts, 1.8 parts, N.F,USP MANNITOL, 59 parts, water.
Embodiment 8
Act on an inductor for yew cell metabolic regulation, be grouped into by the one-tenth of following weight part: Trisodium Citrate 8.5 parts, Plant hormones regulators,gibberellins 5.5 parts, albumin 2.1 parts, luteolin 1.3 parts, dried silkworm chrysalis meal 1.7 parts, yeast powder 1.6 parts, dried orange peel 1.2 parts, Flos Inulae 1.1 parts, Stem of Armand Clematis 1.4 parts, Root-bark of Chinese Silkvine 1.5 parts, Leaf of Indigowoad 1.4 parts, rhamnosyl 1.1 parts, 1.5 parts, N.F,USP MANNITOL, Wrinkled Gianthyssop Herb 1.8 parts, 57 parts, water.
Above embodiments of the invention have been described in detail, but described content is only preferred embodiment of the present invention, not in order to limit the present invention.All make in application range of the present invention any amendment, equivalent to replace and improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. one kind acts on the inductor of yew cell metabolic regulation, it is characterized in that the composition comprising following weight part: Trisodium Citrate 8 ~ 10 parts, Plant hormones regulators,gibberellins 4 ~ 6 parts, albumin 2 ~ 3 parts, luteolin 1 ~ 2 part, dried silkworm chrysalis meal 1 ~ 2 part, yeast powder 1 ~ 2 part, dried orange peel 1 ~ 2 part, Flos Inulae 1 ~ 2 part, Stem of Armand Clematis 1 ~ 2 part, Root-bark of Chinese Silkvine 1 ~ 2 part, 50 ~ 60 parts, water.
2. the inductor acting on yew cell metabolic regulation according to claim 1, characterized by further comprising the Leaf of Indigowoad of 1 ~ 2 weight part.
3. the inductor acting on yew cell metabolic regulation according to claim 1, characterized by further comprising the rhamnosyl of 1 ~ 2 weight part.
4. the inductor acting on yew cell metabolic regulation according to claim 1, characterized by further comprising the N.F,USP MANNITOL of 1 ~ 2 weight part.
5. the inductor acting on yew cell metabolic regulation according to claim 1, characterized by further comprising the Wrinkled Gianthyssop Herb of 1 ~ 2 weight part.
6. the inductor acting on yew cell metabolic regulation according to claim 1, it is characterized in that described inductor is grouped into by the one-tenth of following weight part: Trisodium Citrate 9 parts, Plant hormones regulators,gibberellins 5 parts, albumin 2.5 parts, luteolin 1.5 parts, dried silkworm chrysalis meal 1.5 parts, yeast powder 1.5 parts, dried orange peel 1.5 parts, Flos Inulae 1.5 parts, Stem of Armand Clematis 1.5 parts, Root-bark of Chinese Silkvine 1.5 parts, 55 parts, water.
7. the inductor acting on yew cell metabolic regulation according to claim 1, is characterized in that described inductor is grouped into by the one-tenth of following weight part: Trisodium Citrate 8.5 parts, Plant hormones regulators,gibberellins 5.5 parts, albumin 2.1 parts, luteolin 1.3 parts, dried silkworm chrysalis meal 1.7 parts, yeast powder 1.6 parts, dried orange peel 1.2 parts, Flos Inulae 1.1 parts, Stem of Armand Clematis 1.4 parts, Root-bark of Chinese Silkvine 1.5 parts, Leaf of Indigowoad 1.4 parts, rhamnosyl 1.1 parts, 1.5 parts, N.F,USP MANNITOL, Wrinkled Gianthyssop Herb 1.8 parts, 57 parts, water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510968250.9A CN105400840A (en) | 2015-12-18 | 2015-12-18 | Inducer acting on metabolic regulation of Chinese yew cells |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510968250.9A CN105400840A (en) | 2015-12-18 | 2015-12-18 | Inducer acting on metabolic regulation of Chinese yew cells |
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| CN105400840A true CN105400840A (en) | 2016-03-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510968250.9A Pending CN105400840A (en) | 2015-12-18 | 2015-12-18 | Inducer acting on metabolic regulation of Chinese yew cells |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6428989B1 (en) * | 1997-06-25 | 2002-08-06 | Mitsui Chemicals, Inc. | Method for producing taxane-type diterpenes |
| CN104403987A (en) * | 2015-01-06 | 2015-03-11 | 天津艾赛博生物技术有限公司 | Taxus chinensis cell strain with high yield of 10-DAB and application thereof |
-
2015
- 2015-12-18 CN CN201510968250.9A patent/CN105400840A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6428989B1 (en) * | 1997-06-25 | 2002-08-06 | Mitsui Chemicals, Inc. | Method for producing taxane-type diterpenes |
| CN104403987A (en) * | 2015-01-06 | 2015-03-11 | 天津艾赛博生物技术有限公司 | Taxus chinensis cell strain with high yield of 10-DAB and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| 张磊: "《农业生物技术》", 31 May 2015 * |
| 郝大程: "红豆杉药物资源的生物学和化学研究进展及趋势分析", 《药学学报》 * |
| 陈绍煌: "《药用植物组培快繁实验》", 31 July 2014 * |
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