CN105396541B - A kind of albumen and sugared graft reaction equipment and the preparation method of glycosylation albumen - Google Patents

Abstract

The invention belongs to the technical field of protein deep processing, and in particular relates to a protein and sugar grafting reaction equipment and a preparation method of glycosylated protein. When using this equipment for the grafting reaction of protein and sugar, the reaction substrate enters the reaction chamber from the feeding component, and the pH control component, temperature control component, pressure control component and stirring control component jointly set and adjust the reaction conditions. After the reaction is completed , the reaction product is discharged from the discharge assembly into the post-processing device, and the target glycosylated protein is obtained after processing. Using the equipment provided by the invention to carry out the grafting reaction improves the grafting degree of the protein and sugar grafting reaction, improves the emulsification and solubility of the protein after glycosylation, and solves the problem of long grafting reaction time between the protein and sugar. Low production efficiency and discontinuous response.

Description

A kind of protein and sugar grafting reaction equipment and preparation method of glycosylated protein

technical field

The invention belongs to the technical field of protein deep processing, and in particular relates to a protein and sugar grafting reaction equipment and a preparation method of glycosylated protein.

Background technique

Glycosylation modification is based on the carbonylamino reaction between the free amino group of the amino acid side chain in the protein molecule and the carbonyl group at the reducing end of the sugar molecule - Maillard reaction, which does not require any chemical reagents as a catalyst, and the reaction can be achieved only by heating Do it spontaneously. Mainly used in soy protein, rice protein, whey protein, casein, etc. Judging from the literature reported so far, there are mainly two methods for grafting proteins and sugars: dry heat method and wet heat method, but the reaction time of these two methods is longer, especially the dry reaction, and few Ten hours, as many as a few days or even weeks, is not conducive to application, and the degree of substitution is low. The wet heat method increases the collision probability of protein and sugar, and shortens the time compared with the dry heat method, but it is mainly used for the reaction of small molecular sugars. Therefore, it is very important to invent a kind of grafting reaction equipment with short working time and suitable for grafting proteins with sugars of various molecular weights to obtain highly emulsifiable and soluble proteins.

Contents of the invention

In order to solve the above technical problems, the present invention provides a protein and sugar grafting reaction equipment and a preparation method for glycosylated protein, which improves the degree of grafting of protein and sugar grafting reaction, and improves the glycosylated protein. Emulsification and solubility solve the problems of long reaction time, low production efficiency and discontinuous reaction of protein and sugar grafting.

The present invention is achieved in this way, according to one aspect of the present invention, a kind of protein and sugar grafting reaction equipment is provided, comprising:

The feed assembly consists of a protein feed tube, a sugar feed tube, a distilled water feed tube, a feed regulator and a feed control display. The feed control display is used to set the amount of material required for the reaction, and the set The material amount information is sent to the feed regulator, and the feed controller controls the feed amount of each material in the protein feed pipe, sugar feed pipe, and distilled water feed pipe;

The reaction chamber is connected with the protein feed pipe, the sugar feed pipe and the distilled water feed pipe of the feed assembly;

The pH control component is connected with the reaction chamber to regulate the pH of the reactants in the reaction chamber. It is composed of a pH detector, an alkali feed pipe, an acid feed pipe, a pH regulator and a pH control display. The pH control display is used to set the reaction place. The required pH, the set pH information is sent to the pH controller, and the pH controller compares the pH of the mixed material detected by the pH detector extending into the reaction chamber with the set pH, thereby regulating the pH value from the alkali feed pipe. Add alkali to the material or add acid to the material from the acid feed pipe;

The temperature control component is connected with the reaction chamber to regulate the temperature of the reactants in the reaction chamber. It is composed of an infrared thermometer, a microwave heating component, a circulating water cooling component, a temperature regulator, and a temperature control display. The temperature control display is used to set the reaction place. The required temperature, the set temperature information is sent to the temperature controller, and the temperature controller compares the temperature of the mixed material in the reaction chamber measured by the infrared thermometer with the set reaction temperature, so as to control the microwave heating component to heat the material Or adjust the circulating water cooling component to cool the material; the microwave heating component includes a microwave generating device, a microwave absorbing device and a microwave power control display. The microwave power control display is used to set the microwave power required for the reaction and transmit the required power to the microwave generating device. , the microwave generating device is connected with the reaction chamber to emit microwaves into the reaction chamber, and the microwave absorbing device is arranged on the side wall and the bottom surface of the reaction chamber to absorb the generated microwaves;

The pressure control component is connected with the reaction chamber to regulate the pressure of the reactants in the reaction chamber. It is composed of a pressure gauge, an air supply pipe, an air extraction pipe, a pressure regulator and a pressure control display. The pressure control display is used to set the pressure required for the reaction. The set pressure information is sent to the pressure regulator, and the pressure regulator compares the pressure in the reaction chamber measured by the pressure gauge in the reaction chamber with the set reaction pressure, so as to control the air supply pipe to supply air to the reaction chamber to pressurize or pump The trachea draws air from the reaction chamber to reduce pressure;

The stirring control component is composed of a stirrer, a stirring regulator and a stirring control display. The stirrer is set in the reaction chamber, and the stirring control display is used to set the stirring speed required for the reaction, and transmit the set stirring speed information to the stirring control The stirring regulator controls the speed of the stirrer so as to realize the stirring of the mixed material in the reaction chamber;

The discharge component includes a discharge port, a discharge pipe, a circulation pump and a discharge control display. The discharge port is set at the bottom of the reaction chamber, and the discharge control display is used to set the reaction completion time. When the reaction is completed, the discharge control The monitor controls to open the outlet, and the circulation pump pumps out the reaction product from the reaction chamber; and

The post-processing device is connected with the discharge pipe and is a semi-permeable membrane dialysis device.

Further, the circulating water cooling assembly includes a flow solenoid valve, a heat exchange tube and a refrigeration device. After cooling in the refrigeration device, the circulating water enters the heat exchange tube to exchange heat with the reaction chamber to reduce the reaction temperature. The flow solenoid valve is controlled by the temperature regulator. The regulation controls the flow rate of circulating water.

According to another aspect of the present invention, there is provided a method for utilizing the above-mentioned grafting reaction equipment to carry out protein and sugar grafting reaction, comprising the following steps:

1) Set the amount of protein and distilled water required for the grafting reaction on the feed control display;

2) Set the pH required for preparing the protein solution on the pH control display, and set the stirring speed required for preparing the protein solution on the stirring control display;

3) Start the grafting reaction equipment, make protein solution, and suspend the equipment;

4) Set the amount of sugar required for the grafting reaction on the feed control display, and set the grafting reaction station on the pH control display, temperature control display, microwave power control display, pressure control display, stirring control display and discharge control display. Need pH, temperature, microwave power, pressure, stirring speed and time, start the equipment again, and carry out grafting reaction;

5) After the reaction is over, release the pressure in the reaction chamber through the pressure control display, set the temperature in the reaction chamber to room temperature through the temperature control display, and adjust the pH of the product again through the pH control display, then use the discharge control display to open the discharge port , the circulation pump pumps the reaction product to the dialysis device for dialysis to obtain the grafted reaction product glycosylated protein, which is freeze-dried.

Further, in the above method, the protein is rice protein, the amount of rice protein required for the reaction is 10-50g, the amount of distilled water is 1000-2000mL, the pH required for preparing the protein solution is 12, the stirring speed is 300-700r/min, and the time is 30min ;

The sugar is one of dextran, maltodextrin, glucose, lactose or soluble starch, and the amount of sugar required for the reaction is 15-60g;

The pH required for the grafting reaction is 10.5, the temperature is 80-110°C, the microwave power is 100-800W, the pressure is 40-100MPa, the stirring speed is 300-700r/min, and the reaction time is 10-25min;

The pH of the reaction product was 7.0.

Compared with the prior art, the present invention has the advantages of:

1. The protein and sugar grafting reaction equipment provided by the present invention overcomes the shortcomings of protein physical modification, chemical modification and enzymatic modification, such as long time and slow reaction, and solves the long reaction time and cost of using traditional grafting reactions. higher questions;

2. Proteins and sugars are grafted using the equipment provided by the invention, the degree of grafting is greatly increased, and the emulsification and solubility of the grafts are greatly improved, and the obtained protein-grafted sugars can be used in the food field.

Description of drawings

Below in conjunction with accompanying drawing and embodiment the present invention is described in further detail:

Fig. 1 is the structural representation of protein and sugar grafting reaction equipment provided by the present invention;

Fig. 2 is the connection schematic diagram of each module of equipment provided by the present invention;

Fig. 3 is the Fourier transform infrared spectrum of the glycosylated rice protein obtained in Example 1-5.

detailed description

In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

In order to solve the problems of long time and low efficiency in the preparation of glycosylated proteins by the existing dry and wet methods, and the obtained grafted products have low graft degree, low emulsification and solubility, the present invention provides a protein-sugar grafted Branching reaction equipment and a method for grafting reaction using the device, the device can make the grafting reaction of protein and sugar proceed under the joint action of constant temperature and high pressure and microwave radiation, speed up the reaction rate and reaction process, and improve the yield of the product The degree of grafting improves the solubility and emulsification of the modified protein.

Referring to Figure 1, the protein and sugar grafting reaction equipment provided by the present invention includes a feed assembly 1, a reaction chamber 2, a pH control assembly 3, a temperature control assembly 4, a pressure control assembly 5, a stirring control assembly 6, and a discharge assembly 7 Composed of post-processing device 8, feed assembly 1 is made up of protein feed pipe 11, distilled water feed pipe 12, sugar feed pipe 13, feed regulator 14 and feed control display 15, and feed control display 15 is used for Set the amount of material required for the reaction, and send the set amount of material information to the feed controller 14, and the feed controller 14 controls the protein feed pipe 11, the sugar feed pipe 13, and the distilled water feed pipe that enter the reaction chamber 2. The feed rate of each material in the feed pipe 12;

The pH control assembly 3 is made up of pH probe 31, alkali feed pipe 32, acid feed pipe 33, pH regulator 34 and pH control display 35, and pH control display 35 is used for setting the required pH of reaction, will set pH information sent to the pH controller 34, the pH controller 34 is by comparing the pH of the mixed material detected by the pH detector 31 with the set pH, thereby regulating the addition of alkali or alkali to the material in the reactor 2 from the alkali feed pipe 32 Add acid for the material in the reactor 2 from the acid feed pipe 33;

Temperature control assembly 4 is made up of infrared thermometer 41, microwave heating assembly 42, circulating water cooling assembly 43, temperature controller 44 and temperature control display 45, and temperature control display 45 is used for setting the temperature required for reaction, will set The temperature information is transmitted to the temperature controller 44, and the temperature controller 44 compares the temperature of the mixed material in the reaction chamber 2 measured by the infrared thermometer 41 with the set reaction temperature, thereby regulating the microwave heating component 42 to be in the reaction chamber 2. Heating the material or regulating the circulating water cooling assembly 43 to cool down the material in the reaction chamber 2;

The pressure control assembly 5 is made up of manometer 51, air supply pipe 52, air extraction pipe 53, pressure regulator 54 and pressure control display 55, and pressure control display 55 is used for setting the pressure required for reaction, and the pressure information of setting is transmitted to The pressure regulator 54, the pressure regulator 54 compares the pressure in the reaction chamber 2 measured by the pressure gauge 51 with the set reaction pressure, thereby regulating the air delivery pipe 52 to supply and pressurize the reaction chamber 2 or the air extraction pipe 53 to discharge from the reaction chamber 2 suction and pressure reduction;

Stirring control assembly 6 is made up of stirrer 61, stirring regulator 62 and stirring control display 63, and stirring control display 63 is used for setting the required stirring speed of reaction, and the stirring speed information of setting is passed to stirring regulator 62, and stirring The controller 62 controls the rotation speed of the agitator 61 so as to realize the agitation of the mixed material in the reaction chamber 2;

The discharge assembly 7 includes a discharge port 71, a discharge pipe 72, a circulation pump 73 and a discharge control display 74. The discharge control display 74 is used to set the reaction completion time. After the reaction is completed, the discharge control display 74 controls to open The discharge port 71, the circulation pump 73 extracts the reaction product from the reaction chamber 2;

The post-processing device 8 is connected to the discharge pipe 72 and is a semi-permeable membrane dialysis device.

The microwave heating assembly 42 comprises a microwave generating device 421, a microwave absorbing device 422 and a microwave power control display 423, and the microwave power control display 423 is used to set the microwave power required for the reaction, and the required power for the reaction is transmitted to the microwave generating device 421, and the microwave generating device 421 is connected to the reaction chamber 2 and emits microwaves into the reaction chamber 2. The microwave absorbing device 422 is arranged on the side wall and bottom surface of the reaction chamber 2 to absorb the generated microwaves.

The circulating water cooling assembly 43 includes a flow solenoid valve 431, a heat exchange tube 432 and a refrigeration device 433. After cooling in the refrigeration device 433, the circulating water enters the heat exchange tube 432 to exchange heat with the reaction chamber 2 to reduce the reaction temperature. The flow solenoid valve 431 is controlled by the temperature regulator 44 to control the flow rate of the circulating water.

During the working state, the feed assembly 1 is connected to each material reservoir respectively; the acid-base feed pipe of the pH control assembly 3 is respectively connected with the acid-base storage; When increasing the pressure in the reaction chamber 2, it is completed by sending in water vapor, and the air extraction pipe 53 is connected with the air extraction pump.

The mains supply the device with power. Referring to Figure 2, each control display is connected in parallel, and each control display is connected in series with the downstream module it controls to complete the control of the entire device.

When using this equipment to carry out the grafting reaction of protein and sugar, the reaction substrate enters the reaction chamber 2 from the feed component 1, and the pH control component 3, the temperature control component 4, the pressure control component 5 and the stirring control component 6 are jointly set and adjusted Reaction conditions, after the reaction is completed, the reaction product is discharged from the discharge component 7 into the post-processing device 8, and the target glycosylated protein is obtained after processing.

Example 1

1) Set the amount of rice protein required for the grafting reaction on the feed control display to be 10g, and the amount of distilled water to be set to 1000mL;

2) Set the pH required for preparing the protein solution on the pH control display to 12, and set the stirring speed required for preparing the protein solution on the stirring control display to 300r/min;

3) Start the grafting reaction equipment, the reaction time is 30min, and suspend the equipment after making protein solution;

4) The amount of sugar required for the grafting reaction is set to 30g on the feed control display, and the connection is set on the pH control display, temperature control display, microwave power control display, pressure control display, stirring control display and discharge control display. The pH required for the branching reaction is 10.5, the temperature is 110°C, the microwave power is 100W, the pressure is 100MPa, the stirring speed is 300r/min, and the reaction time is 10min. Start the equipment again to carry out the grafting reaction;

5) After the reaction is over, release the pressure in the reaction chamber through the pressure control display, set the temperature in the reaction chamber to room temperature through the temperature control display, adjust the pH of the product to 7.0 again through the pH control display, and use the discharge control display to open the discharge The circulation pump pumps the reaction product to the dialysis device for dialysis for 24 hours to obtain the grafted reaction product glucose glycosylated rice protein, which is freeze-dried.

Example 2

The difference from Example 1 is:

In step 1), the amount of rice protein added is set to 20g, the amount of distilled water is set to 1500mL, and the stirring speed of the protein liquid is 400r/min;

The sugar added in step 4) is maltodextrin, the added amount is set to 60g, the reaction temperature is 100°C, the microwave power is 300W, the pressure is 80MPa, the stirring speed is 400r/min, and the reaction time is 15min.

Example 3

The difference from Example 1 is:

In step 1), the amount of rice protein added is set to 10g, the amount of distilled water is set to 1500mL, and the stirring speed of the protein liquid is 500r/min;

The sugar added in step 4) is glucose, the amount added is 20g, the reaction temperature is 90°C, the microwave power is 500W, the pressure is 60MPa, the stirring speed is 500r/min, and the reaction time is 25min.

Example 4

In step 1), the amount of rice protein added is set to 20g, the amount of distilled water is set to 1500mL, and the stirring speed of making protein liquid is 600r/min;

The sugar added in step 4) is maltodextrin, the added amount is set to 60g, the reaction temperature is 100°C, the microwave power is 300W, the pressure is 80MPa, the stirring speed is 600r/min, and the reaction time is 15min.

Example 5

In step 1), the amount of rice protein added is set to 50g, the amount of distilled water is set to 2000mL, and the stirring speed of the protein liquid is 700r/min;

The sugar added in step 4) is lactose, the amount added is 50g, the reaction temperature is 80°C, the microwave power is 800W, the pressure is 40MPa, the stirring speed is 700r/min, and the reaction time is 15min.

The degree of grafting of the glycosylated rice protein obtained in embodiment 1, embodiment 2, embodiment 3, embodiment 4 and embodiment 5, pH is that 7 temperature is that solubility, emulsification under 25 ℃ are detected,

The detection method of grafting degree is as follows:

The degree of grafting of glycosylated proteins was determined by OPA method for the determination of free amino groups. The specific method is as follows: accurately weigh 40mg OPA and dissolve it in 1mL methanol, add 20% (w/v) SDS 2.5mL, 0.1mol/L borax 25mL and 100uL β-mercaptoethanol respectively, and finally distilled water to volume 50ml, this is OPA reagent, and it is prepared immediately before use. When measuring, measure 4mL of OPA reagent into a test tube, inject 200uL of sample solution (5mg/mL), mix well and react at 35°C for 2min, measure its absorbance A ₃₄₀ at 340nm, and add 200uL of water to the OPA reagent. blank. Make a standard curve with lysine, calculate the content of free amino groups in the sample according to ΔA, and calculate the degree of grafting (DG):

DG＝(C ₀ -C _t )/C ₀ ×100%

Among them: C ₀ : the total amount of free amino groups of protein when unreacted;

C _t : the content of free amino groups in the protein at time t of the reaction

The solubility test method is as follows:

Disperse 0.5g protein sample in 20mL distilled water, stir on a magnetic stirrer for 2h, transfer to a 25mL volumetric flask to constant volume, take 9mL in a 10mL centrifuge tube, centrifuge at 7000rpm for 10min, accurately absorb 0.1mL of the supernatant, and follow Bradford Determination by method, using bovine serum albumin (BSA) as a standard curve, measuring the absorbance at 595nm, calculating the solubility according to the protein content in the sample and the protein content in the solution, expressed as nitrogen solubility index (NSI%).

The emulsifying test method is as follows:

Using the turbidimetric method Pearce et al. (1978), the specific steps are as follows: prepare 25 mL of a 0.1% (m/v) sample solution in a pH 7.0, 0.1 mol/L phosphate buffer solution. Take 25mL sample solution and add 8mL soybean oil, and homogenize in a high-speed homogenizer at 10000rpm for 1min at room temperature to form an emulsion. After homogenization, draw 100 μL of sample from the bottom at 0 min and 10 min respectively, add 10 mL of 0.1% SDS (m/v) to dilute, measure the absorbance value at 500 nm (measured three times), and the emulsification activity index (Emulsification Activity Index, EAI) according to The following formula is calculated:

In the formula:

EAI(m ² /g): N dilution factor Fraction of oil phase in the system

C: protein concentration (g/ml)

L: is the light path of the colorimetric cell (1cm)

Emulsification Stability Index (ESI) is calculated according to the following formula:

ESI＝(A ₀ ×ΔT)/(A ₀ -A ₁₀ )

In the formula:

ESI: Emulsion Stability Index, min;

A ₀ : Absorbance value at 0 min;

ΔT: the time interval between two times of emulsification measurement, 10min is used in this test;

A ₁₀ : Absorbance value at 10 minutes, the test results are shown in the table below:

Table 1, embodiment product detection result

From the results in the above table, it can be seen that under the microwave and high pressure conditions of this equipment, the grafting reaction time of rice protein and sugar is reduced to 10-20 minutes, the degree of grafting can reach 21-31%, and the solubility of the graft can reach 63.58% at the same time. % to 83.58%, 10% to 56.6% higher than unmodified rice protein, emulsifying property up to 22.16, 31.8% to 48.1% higher than unmodified rice protein, emulsifying stability up to 51.51, which is 24.1%-62.2% higher than that of unmodified rice protein, achieving the purpose of accelerating the reaction time of sugar grafting and changing the solubility and emulsification of rice protein.

Fourier infrared detection:

Referring to Figure 3, it is the collection of spectra obtained by Fourier transform infrared scanning of the glycosylated rice protein obtained in Example 1, Example 2, Example 3, Example 4 and Example 5. As can be seen from the figure, using Fourier Leaf infrared spectroscopy, the graft FT-IR spectrum is analyzed, it can be seen that the infrared spectrum of the rice protein grafted with sugar not only has the characteristic peak of the absorption band of the phthaloamide compound of the protein, but also has the characteristic absorption of sugar Peak, the composite product has a wider absorption band formed by O-H and N-H near 3350cm-1, this is due to the new N-H bond in the covalent cross-linking reaction of the new substance, there is at 1651cm-1 (amide I) The strong C=O stretching vibration indicates that the group has reacted here, and this vibration can form a β-rotation angle. The stretching vibration of the 1539 (amide II) N-H bond is enhanced, and there is an N-H variable angle vibration, which belongs to the β-sheet. Composed of extended polypeptide chains, the peak at 1153cm-1 (amide III) belongs to the α-helix and random coil formed by N-H variable angle vibration and C-N stretching vibration; at the same time, there is a strong absorption at 1020cm-1, This may be due to the O-H bending vibration caused by the access of the hydroxyl group. Through the analysis of the infrared spectrum of the product, it can be inferred that the structure of the rice protein can be changed through this device and the grafting reaction between the rice protein and the sugar has occurred. Combined to form graft copolymers.

Claims (2)

1. a method utilizing grafting reaction equipment to carry out protein and sugar grafting reaction, it is characterized in that, described grafting reaction equipment comprises: The feed assembly consists of a protein feed tube, a sugar feed tube, a distilled water feed tube, a feed regulator and a feed control display. The feed control display is used to set the amount of material required for the reaction, and the set The material amount information is sent to the feed controller, and the feed controller controls the feed amount of each material in the protein feed pipe, sugar feed pipe, and distilled water feed pipe; The reaction chamber is connected with the protein feed pipe, the sugar feed pipe and the distilled water feed pipe of the feed assembly; The pH control assembly is connected with the reaction chamber to regulate the pH of the reactants in the reaction chamber, and is composed of a pH detector, an alkali feed pipe, an acid feed pipe, a pH regulator and a pH control display, and the pH control display is used for Set the pH required for the reaction, and transmit the set pH information to the pH regulator, and the pH regulator compares the pH of the mixed material detected by the pH probe inserted into the reaction chamber with the set pH, thereby Adding alkali to the material from the alkali feed pipe or adding acid to the material from the acid feed pipe; The temperature control assembly is connected with the reaction chamber to regulate the temperature of the reactants in the reaction chamber, and is composed of an infrared thermometer, a microwave heating assembly, a circulating water cooling assembly, a temperature controller, and a temperature control display. The temperature control display is used for Set the temperature required for the reaction, and transmit the set temperature information to the temperature controller. The temperature controller controls the microwave heating by comparing the temperature of the mixed material in the reaction chamber measured by the infrared thermometer with the set reaction temperature. The component is for material heating or the circulating water cooling component is for material cooling; the microwave heating component includes a microwave generating device, a microwave absorbing device and a microwave power control display, and the microwave power control display is used to set the microwave power required for the reaction, and the reaction required The power is transmitted to the microwave generating device, the microwave generating device is connected to the reaction chamber, and emits microwaves into the reaction chamber, and the microwave absorbing device is arranged on the side wall and the bottom surface of the reaction chamber to absorb the generated microwaves; The pressure control assembly is connected with the reaction chamber to regulate the pressure of the reactants in the reaction chamber, and is composed of a pressure gauge, an air supply pipe, an air extraction pipe, a pressure regulator and a pressure control display, and the pressure control display is used to set the reaction requirements. The pressure of the set pressure is transmitted to the pressure regulator, and the pressure regulator compares the pressure in the reaction chamber measured by the pressure gauge in the reaction chamber with the set reaction pressure, thereby regulating the gas supply pipe Pressurize the reaction chamber with air supply or pump the air from the reaction chamber to reduce the pressure; The stirring control assembly is composed of a stirrer, a stirring regulator and a stirring control display. The stirrer is arranged in the reaction chamber, and the stirring control display is used to set the stirring speed required for the reaction, and transmit the set stirring speed information to Stirring regulator, the stirring regulator controls the rotation speed of the agitator so as to realize the stirring of the mixed material in the reaction chamber; The discharge assembly includes a discharge port, a discharge pipe, a circulation pump and a discharge control display. The discharge port is arranged at the bottom of the reaction chamber, and the discharge control display is used to set the reaction completion time. When the reaction is completed, the discharge The material control display controls to open the discharge port, and the circulation pump extracts the reaction product from the reaction chamber; and The post-processing device is connected with the discharge pipe and is a semi-permeable membrane dialysis device; The method comprises the steps of: 1) Set the amount of protein and distilled water required for the grafting reaction on the feed control display; 2) Set the pH required for preparing the protein solution on the pH control display, and set the stirring speed required for preparing the protein solution on the stirring control display; 3) Start the grafting reaction equipment, make protein solution, and suspend the equipment; 4) Set the amount of sugar required for the grafting reaction on the feed control display, and set the grafting reaction station on the pH control display, temperature control display, microwave power control display, pressure control display, stirring control display and discharge control display. Need pH, temperature, microwave power, pressure, stirring speed and time, start the equipment again, and carry out grafting reaction; 5) After the reaction is over, release the pressure in the reaction chamber through the pressure control display, set the temperature in the reaction chamber to room temperature through the temperature control display, and adjust the pH of the product again through the pH control display, then use the discharge control display Open the discharge port, and the circulation pump pumps the reaction product to the dialysis device for dialysis to obtain the grafted reaction product glycosylated protein, which is freeze-dried; Wherein, the protein is rice protein, the amount of rice protein required for the reaction is 10-50g, the amount of distilled water is 1000-2000mL, the pH required for preparing the protein solution is 12, the stirring speed is 300-700r/min, and the time is 30min; The sugar is one of dextran, maltodextrin, glucose, lactose or soluble starch, and the amount of sugar required for the reaction is 15-60g; The pH required for the grafting reaction is 10.5, the temperature is 80-110°C, the microwave power is 100-800W, the pressure is 40-100MPa, the stirring speed is 300-700r/min, and the reaction time is 10-25min; The pH of the reaction product was 7.0. 2. the method utilizing grafting reaction equipment to carry out albumen and sugar grafting reaction according to claim 1, is characterized in that, described circulating water cooling assembly comprises flow solenoid valve, heat exchange tube and refrigeration unit, and circulating water is in the After being refrigerated in the refrigerating device, it enters the heat exchange tube to exchange heat with the reaction chamber to reduce the reaction temperature, and the flow solenoid valve is regulated by the temperature controller to control the flow rate of circulating water.