CN105392468A - Preparation method of mussel adhesive protein gel, mussel adhesive protein gel and use thereof - Google Patents
Preparation method of mussel adhesive protein gel, mussel adhesive protein gel and use thereof Download PDFInfo
- Publication number
- CN105392468A CN105392468A CN201380076471.4A CN201380076471A CN105392468A CN 105392468 A CN105392468 A CN 105392468A CN 201380076471 A CN201380076471 A CN 201380076471A CN 105392468 A CN105392468 A CN 105392468A
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- Prior art keywords
- sea
- gel
- mussel mucin
- preparation
- mussel
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- 230000001988 toxicity Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
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- A61K35/56—Materials from animals other than mammals
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1767—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/0005—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/10—Polypeptides; Proteins
- A61L24/108—Specific proteins or polypeptides not covered by groups A61L24/102 - A61L24/106
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J189/00—Adhesives based on proteins; Adhesives based on derivatives thereof
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Abstract
The present invention provides a preparation method of mussel adhesive protein gel, comprising: acquiring or preparing mussel adhesive protein, acquiring or preparing gel matrix, admixing the gel matrix and liquid mussel adhesive protein, and obtaining mussel adhesive protein gel. The present invention also provides the mussel adhesive protein gel and uses thereof in wound surface repairing product, wound surface protecting product, medical biological adhesive product, medical coating product, industrial coating product and cosmetics etc.
Description
Preparation method, sea-mussel mucin gel and its applied technical field of sea-mussel mucin gel
It is that main active prepares the method that wound repair product, protecting wound surface product, old scar dispel product, adhesive product, embedded type reparation product, the moisturizing class cosmetics for increasing skin elasticity, anticorrosion medical coating and industrial coating product the present invention relates to the preparation method of sea-mussel mucin gel-like product, more particularly to using sea-mussel mucin.
Background technology
Sea-mussel mucin（Mussel Adhesive Proteins, MAP) or " mussel byssus protein "（Mytilus Edulis Foot Protein MEFP), it is a kind of polyhydric phenols basic protein derived from marine mussel.Mussel is commonly called as " Hai Hong ", " mussel ", belongs to cool temperature zone, eurysalinity species, in global each marine site with the presence of variety classes, China there are about 50 kinds.It is distributed in the Perna viridis of Perenniporia martius（Perna viridis), be distributed in the Mytilus galloprovincialis in frigid zone and temperate zone（Mytilus edulis) and Trachyostracous mussel（Mytilus coruscus) it is the important cultivation in countries in the world and fished species.Sea-mussel mucin is present in a special body of gland for mussel, the glycosylation modified of complexity, hydroxylating modification and cross-linking reaction is experienced in synthesis, just because of the special the Nomenclature Composition and Structure of Complexes of this albumen so that mussel has extremely special ability.There is L-3,4- dihydroxyphenylalanines in sea-mussel mucin（L-DOPA), L-DOPA residues are cross-linked with each other due to oxidation reaction so that generate strong and lasting splicing in mussel and special surface fiber.Sea-mussel mucin has the advantages that bonding scope is wide, speed is fast, corrosion-resistant, intensity is high and bioaffinity is good, it is considered to be fabulous broad-spectrum biological adhesive and applied to fields such as medical science and bioengineering.Moreover, recent studies suggest that, sea-mussel mucin can effectively facilitate burn, scald skin healing and growth, also have obvious effect to dispelling for outmoded scars.
Sea-mussel mucin generally exists with liquid or freeze-dried powder state, when using by the way of direct smearing or spraying, but during for privileged sites such as position in irregular shape, it is not easy to keep, action effect is uneven.And liquid rate of drying is fast, the action time of sea-mussel mucin is shortened, reduces the effect of sea-mussel mucin.
Gel be by active material and gel-type vehicle be made it is homogeneous, be suspended or emulsion-type latex glop or semisolid preparation, with good biocompatibility, be easy to absorb after local administration, not pollution clothes, stability it is good, enable in particular to produce slow-release controlled-release and pulsed release effect.Gel substrate belongs to single-phase decentralized system, has aqueous point with oiliness, aqueous gel matrix is typically made up of water, glycerine or propane diols and cellulose derivative, carbomer and alginate, tragacanth, gelatin, starch etc.；Oil-base gel matrix is by liquid
Shape paraffin is constituted with polyethylene or fat oil with colloidal silicon or aluminium soap, zinc soap, in recent years the research and development focus as exterior-applied formulation.
In addition, China Patent Publication No.（CN101348520, CN101348518, CN101585874) and PCT international application no（PCT/CN2012/073167 the method for several separating and purifying sea-mussel mucins disclosed in), is hereby incorporated, and belongs to the content that the present invention is recorded.
The content of the invention
It is an object of the invention to provide a kind of preparation method of sea-mussel mucin gel, prepared sea-mussel mucin gel and its application, to solve the defect for being difficult to keep that prior art is directly smeared or spraying is brought using liquid sea-mussel mucin or its fluid product.
To achieve the above object, the present invention proposes a kind of preparation method of sea-mussel mucin gel, including：The rapid h of Walk obtain or prepared sea-mussel mucin；
Walk rapid 2:Obtain or prepare gel-type vehicle；
Walk rapid 3:The gel-type vehicle is mixed with the liquid sea-mussel mucin, sea-mussel mucin gel is obtained.
Wherein, the preparation method of the sea-mussel mucin gel also includes：
Walk rapid 4:Gel-type vehicle described in Yu Walk rapid 3 adds auxiliary material after being mixed with the liquid sea-mussel mucin, the auxiliary material is one of nertralizer, solubilizer, NMF, antioxidant, bacteriostatic agent, penetrating agent or aromatic or its any combination.
Wherein, in Suo Shu Walk rapid 1, the sea-mussel mucin is liquid sea-mussel mucin or is the liquid sea-mussel mucin that dry powder state sea-mussel mucin is obtained through the dissolving of acid or neutral solution.
Wherein, it is to mix gel matrix material with water to prepare gel-type vehicle in Suo Shu Walk rapid 2, and gel-type vehicle is formed after being swelled.
It is preferred that the gel matrix material accounts for the gel-type vehicle mass percent for 0. 01-5%.
More preferably, the gel matrix material accounts for the mass percent of the gel-type vehicle for 0. 08-5% wherein, and acquired gel-type vehicle is gel matrix material in Suo Shu Walk rapid 2.
Wherein, the gel matrix material is one of cellulose derivative, carbomer and alginate, tragacanth, gelatin, pectin, carragheen, gellan gum, starch, xanthans, cation guar gum, agar, non-cellulosic polysaccharide, ethene polymers, acrylic resin, polyvinyl alcohol or carbopol or its any combination.
In wherein , Yu Walk rapid 3, the hybrid mode of the sea-mussel mucin and the gel-type vehicle is to smear or spray the sea-mussel mucin on the gel-type vehicle to form the sea-mussel mucin gel.
In wherein , Yu Walk rapid 3, the hybrid mode of the sea-mussel mucin and the gel-type vehicle is directly to be swelled the gel rubber material with sea-mussel mucin solution to form the sea-mussel mucin gel.
Wherein, the content of sea-mussel mucin described in the sea-mussel mucin gel is 0. lmg/g-10mg/g.Wherein, the ratio that the auxiliary material accounts for sea-mussel mucin gel gross mass is 0-50%, and the pH during auxiliary material addition is 1. 0-9. 0.
In addition, the invention also provides the sea-mussel mucin gel obtained by the preparation method of above-mentioned sea-mussel mucin gel.
In addition, the application the invention also provides above-mentioned sea-mussel mucin gel in wound repair product, protecting wound surface product, medical bio adhesive product, medical coating product, industrial coating product, biochemical reagents product, medical product, sterile products, cell culture culture vessel.
Technique effect：
Sea-mussel mucin gel products can play the advantage of sea-mussel mucin and gel simultaneously, and obtained sea-mussel mucin gel is easily kept, effect is lasting, action effect is uniform, long action time, good dispersion, adhesive force is strong, stability is good, it is simple, easy to use to prepare, be readily cleaned.Made product can be as amenities is for the reparation of scar on the outmoded surface of a wound and dispels；As used for cosmetic in the moisturizing moisturizing of skin and smoothing wrinkle；It is used for the reparation of damaged skin and mucous membrane as wound repair material；It is used for the protection of the surface of a wound as protecting wound surface product；It is used for the sustained release of skin, body cavity, rectal application as sustained release preparation；It is used for the bonding of wound as medical bio adhesive product；It is used for anti-corrosion or closure type support as medical coating product, industrial coating product；It is used for periodontitis, dentistry implant as dental material；And it is used as cell culture culture products etc..
Brief description of the drawings
Fig. 1 is that sea-mussel mucin gel Bei Fang Fa Walk of the present invention scheme suddenly.
It is male:^
Gel has the advantages that good dispersion, adhesive force are strong, stability is good, effect is lasting, it is simple to prepare, it is easy to use, be readily cleaned, gel matrix material and albumen that present invention selection is not reacted with sea-mussel mucin
New sea-mussel mucin product is made, the effect of sea-mussel mucin can be preferably played.
The present invention proposes the preparation method of sea-mussel mucin gel, is prepared for the medical and industrial sea-mussel mucin gel products by raw material of sea-mussel mucin.It is used as wound repair product, protecting wound surface product, medical bio adhesive product, medical coating product, industrial coating product, biochemical reagents product, medical product, sterile products, cell culture culture vessel etc..Available for burning, scald, ulcer, pernio, bedsore, the reparation of the various skins such as operation and the mucous membrane surface of a wound；The implantation of artificial bone, cartilage frame, periosteum, joint prosthesis, dentistry implant, seal-off effect support, spine cage, vertebral spacing device, organ sticking patch etc.；The protection of the various mucocutaneous surface of a wound such as burn, scald, ulcer, pernio, bedsore, operation；The closure of operative incision；The bonding of fracture；The bonding of mucous membrane；The body implant coatings such as artificial bone, cartilage frame, periosteum, joint prosthesis, dentistry implant, seal-off effect support, spine cage, vertebral spacing device, organ sticking patch；Cell and tissue culture；The anticorrosion of ship, electronic equipment, pipeline etc..
Technical scheme（Such as Fig. 1)：
The present invention proposes a kind of preparation method of sea-mussel mucin gel, it is characterised in that including：Walk rapid 1:Obtain or prepare sea-mussel mucin；
Walk rapid 2:Obtain or prepare gel-type vehicle；
Walk rapid 3:The gel-type vehicle is mixed with the liquid sea-mussel mucin, sea-mussel mucin gel is obtained.Wherein, in Suo Shu Walk rapid 1, the sea-mussel mucin is liquid sea-mussel mucin or is the liquid sea-mussel mucin that dry powder state sea-mussel mucin is obtained through the dissolving of acid or neutral solution.
Jin mono- Walk, acid or neutral solution pH value is 1.0-7.0, including：Inorganic acid, such as hydrochloric acid, phosphoric acid, nitric acid, boric acid, perchloric acid, periodic acid, permanganic acid；Organic acid, such as：Formic acid, benzoic acid, acetic acid, ethanedioic acid, citric acid etc.；The buffer solution of weak acid and its salt：Acetic acid-acetate buffer, phosphoric acid-phosphate buffer, boric acid-borate, citric acid-citrate etc..
The sea-mussel mucin can be any one in 8 subclass：Sea-mussel mucin 1-6 (Mytilus edulis foot protein, mefp-l, mei 3-2, mefp-3 mefp-4, mei 3-5, π ι β -), standing grain mouthful collagen preCoID, preCoIN.
Wherein, the preparation of gel-type vehicle is to mix gel matrix material with water in Suo Shu Walk rapid 2, is obtained after being swelled.The mass percent that the gel matrix material accounts for the gel is 0.01-5%, in preferably 0.08-5% Qie Yu Walk rapid 3, the hybrid mode of the sea-mussel mucin and the gel-type vehicle, which is included on the gel-type vehicle, smears or sprays the sea-mussel mucin；Or in , Yu Walk rapid 3, the hybrid mode of the sea-mussel mucin and the gel-type vehicle is directly to be swelled the gel rubber material with sea-mussel mucin solution to form the sea-mussel mucin gel.
Jin mono- Walk enter, the gel-type vehicle preparation time be it is any, preferably 10-120 minutes.
Jin mono- Walk enter, the gel-type vehicle preparation temperature be it is any, preferably 10-80 °C.
Jin mono- Walk, the gel matrix material can be a kind of matrix be used alone or two kinds or it is several between compounding be used in mixed way, i.e., the gel matrix material can be selected from one of following or any combination, and including following but not limited to this：Cellulose derivative（Carboxymethyl cellulose, sodium carboxymethylcellulose, hydroxypropyl methyl cellulose, hydroxypropyl methyl cellulose sodium etc.）, carbomer and alginate, tragacanth, gelatin, pectin, carragheen, gellan gum, starch, xanthans, cation guar gum, agar and non-cellulosic polysaccharide, such as chitin, ethene polymers, acrylic resin, polyvinyl alcohol and carbopol.
Wherein, the content of sea-mussel mucin described in the sea-mussel mucin gel is 0. lmg/g-10mg/g.According to the present invention, preferable Hai Bao Kuo Walk rapid 4:Gel-type vehicle described in Yu Walk rapid 3 adds auxiliary material after being mixed with the liquid sea-mussel mucin, the auxiliary material is one of nertralizer, solubilizer, NMF, antioxidant, bacteriostatic agent, penetrating agent, aromatic or its any combination.
Jin mono- Walk, nertralizer includes triethanolamine, hydrochloric acid, sodium hydroxide, sodium acid carbonate, sodium dihydrogen phosphate, but is not limited only to this.Solubilizer includes Tween-80, polyoxyethylene, rilanit special, but is not limited only to this.NMF includes glycerine, propane diols, sorbierite, but is not limited only to this.Antioxidant includes cysteine, sulphite, but is not limited only to this.Bacteriostatic agent includes chloreresol, thimerosal, carboxyl ethanol, benzoic acid, benzyl chloride protective embankment ammonia, but is not limited only to this.Penetrating agent includes azone, oleyl alcohol, oleic acid, carbitol, ethanol, but is not limited only to this.Aromatic includes rose essential oil, peppermint, but is not limited only to this.Bactericide includes PVP-I, silver iodide, Nano Silver, but is not limited only to this.The implementation of auxiliary material process is added, can also can be implemented with implementing in the 2nd Walk formation gel process or after forming gel in the 3rd Walk formation sea-mussel mucin gel process or after forming sea-mussel mucin gel；It can not select to implement, can also select to implement one or more until all implementing；It if selection implements more than two, can individually implement, first another rise after each auxiliary materials and mixing can also be implemented.
Jin mono- Walk, the adding proportion of the auxiliary material is 0-50%, preferably 1-30%.
Jin mono- Walk, the pH during addition of the auxiliary material is 1.0-9.0, preferably ρ Η 1.0-9.0.
Jin mono- Walk, the incorporation time of the auxiliary material is any, preferably 5-60 min.
Jin mono- Walk, during the mixing of the auxiliary material temperature be it is any, preferably 10-50 °C.
In addition, the invention also provides the sea-mussel mucin gel obtained by the preparation method of above-mentioned sea-mussel mucin gel.
Jin mono- Walk, the present invention proposes sea-mussel mucin gel in wound repair product, protecting wound surface product, doctor
With the application in bioadhesive product, medical coating product, industrial coating product, biochemical reagents product, medical product, sterile products, cell culture culture vessel.Embodiment
Embodiment 1
1 prepares sea-mussel mucin solution, and concentration is 5mg/mL-60mg/mL, using acetic acid-acetate buffer, and pH is 1-9.
The 2 accurate injection waters for weighing 3g cation guar gums, adding 85mL, stir, 10-120min are placed in 10 °C -80 °C of environment, form gel.
3 add the lOOmg prepared in Shang Shu Walk rapid 1 sea-mussel mucin into Walk rapid 2, make its final concentration of lmg/g, stir, add water and complement to 100g, form final sea-mussel mucin gel.
The appropriate gel coating is taken in the surface of joint prosthesis, then implantation within a patient, the presence of sea-mussel mucin gel contributes to the growth of implant site cell and tissue, reduces the immune response of human body, improves success rate.The sea-mussel mucin gel all the components are all degradable in human body, after its efficacy exertion is finished, it will thoroughly degraded in human body, do not have toxicity to human body, without any excitant and adverse reaction.Embodiment 2
1 prepares sea-mussel mucin solution, and concentration is 5mg/mL-50mg/mL, using citric acid solution, and pH is 1-9.
The 2 accurate injection waters for weighing lg carbomers, adding 65mL, stir, and adjusted using triethanolamine and 10-120min is placed in pH to 6-7,10 °C -80 °C of environment, form gel.
3 add the 150mg prepared in Shang Shu Walk rapid 1 sea-mussel mucin into Suo Shu Walk rapid 2, make its final concentration of 1.5mg/g, stir, and form sea-mussel mucin gel.
4 add 15mL glycerine into Suo Shu Walk rapid 3, stir, add water and complement to 100g, uniform mixing, form final sea-mussel mucin gel.
Take the appropriate gel coating in the surface of old scar, smear uniform, whole scar is completely covered, a gel was changed again at interval of 6-8 hours, a course for the treatment of is adhered to（About 20 days）, old scar will be substantially thin out, is further continued for two courses for the treatment of, better.Embodiment 3
1 prepares sea-mussel mucin solution, and concentration is 10mg/mL-50mg/mL, using phosphoric acid solution, and pH is 1-9.
The 2 accurate injection waters for weighing 2g hydroxypropyl methyl celluloses, adding 65mL, stir, 10 °C
10-120min is placed in -80 °C of environment, gel is formed.
3 add the 200mg prepared in Shang Shu Walk rapid 1 sea-mussel mucin into Suo Shu Walk rapid 2, make its final concentration of 2mg/g, stir, and form sea-mussel mucin gel.
4 are separately added into 10mL sorbierites into Suo Shu Walk rapid 3, and 5mL glycerine, 4g PVP-Is, O.Olg silver iodide stir, add water and complement to 100g, uniform mixing, form final sea-mussel mucin gel.
Particularly, in the tetra- Walk in above-described embodiment 3.10mL sorbierites, 5mL glycerine, 4g PVP-Is, O.Olg silver iodide can be together added in 20mL injection waters, after being well mixed, are added in sea-mussel mucin gel, then form final sea-mussel mucin gel after uniform mixing.
This appropriate gel coating is taken at newborn incised wound or place of damaging, smear uniform, whole injury is completely covered, a gel was changed again at interval of 8-12 hours, the presence of sea-mussel mucin can significantly reduce the bleeding of wound, the healing of wound is effectively facilitated, while the presence of PVP-I and silver iodide plays the role of sterilization to wound, sea-mussel mucin can then reduce excitant of these bactericide to wound skin.Adhere to course for the treatment of (7 days）, can well be healed and be recovered with damaged part.Embodiment 4
1 dissolving sea-mussel mucin dry powder, obtains sea-mussel mucin solution, concentration is 10mg/mL-50mg/mL, using acetic acid-acetate buffer, and pH is 1-9.
The 2 accurate injection waters for weighing lg hydroxypropyl methyl celluloses, adding 60mL, stir, 10-120min are placed in 10 °C -80 °C of environment, form gel.
3 add the 300mg prepared in Shang Shu Walk rapid 1 sea-mussel mucin into Suo Shu Walk rapid 2, make its final concentration of 3mg/g, by 12mL sorbierites, 2mL glycerine, lmL propane diols, O.Olg thimerosals, O.OOlg rose essential oils, are added in 20mL injection waters together, after being well mixed, it is added in sea-mussel mucin gel, then forms final sea-mussel mucin gel after uniform mixing.
This appropriate gel coating is taken at the skin of scald, smear uniform, it is completely covered at whole scald, above-mentioned gel was changed once again at interval of 10-12 hours, the gel can not only keep smearing the wettability at position, give patient a kind of comfortable body-sensing, and because aeration is good and sea-mussel mucin biological characteristics in itself, the gel can effectively facilitate the recovery at scald.Adhere to three courses for the treatment of（30 days altogether）, can well be healed and be recovered with damaged part.Embodiment 5
1 prepares sea-mussel mucin solution, and concentration is 15mg/mL-60mg/mL, using acetic acid-acetate buffer, and pH is 1-9.
The 2 accurate injection waters for weighing lg hydroxypropyl methyl celluloses and 2g cation guar gums, adding 50mL, are stirred, stir, and pH to 6-7 is adjusted using triethanolamine, and 10 °C -8 (is placed 10-120min, form gel in TC environment.
3 add the addition 250mg prepared in Shang Shu Walk rapid 1 sea-mussel mucin into Suo Shu Walk rapid 3, make its final concentration of 2.5mg/g, by 10mL sorbierites, 2mL glycerine, 3mL propane diols, 0.02g chloreresols, 0. lg sodium sulfites, are added in 25mL injection waters together, after being well mixed, it is added in sea-mussel mucin gel, then forms final sea-mussel mucin gel after uniform mixing.
4 by 10mL sorbierites, 2mL glycerine, 3mL propane diols, 0.02g chloreresols, 0. lg sodium sulfites, 5g PVP-Is are added in 25mL injection waters together, after well mixed, it is added in sea-mussel mucin gel, then forms final sea-mussel mucin gel after uniform mixing.
This appropriate gel coating is taken at the skin of burn, smear uniform, whole burn site is completely covered, above-mentioned gel was changed once again at interval of 8-12 hours, the gel can not only keep smearing the wettability at position, give patient a kind of comfortable body-sensing, and the microorganism of wound can be killed, give wound one relatively good recovery environment, moreover, the biological characteristics of the good gas permeability of the gel and sea-mussel mucin, the gel in itself can effectively facilitate the recovery of burned part.Adhere to three courses for the treatment of（60 days altogether）, can well be healed and be recovered with burned part.Embodiment 6
1 dissolving sea-mussel mucin dry powder, obtains sea-mussel mucin solution, concentration is 20mg/mL-50mg/mL, using citric acid-citrate buffer solution, and pH is 1-9.
The 2 accurate injection waters for weighing lg hydroxypropyl methyl celluloses and 2g cation guar gums, adding 50mL, are stirred, stir, and pH to 5-7 is adjusted using sodium hydroxide, and 10 °C -8 (is placed 10-120min, form gel in TC environment.
3 add the addition 300mg prepared in Shang Shu Walk rapid 1 sea-mussel mucin into Suo Shu Walk rapid 2, make its final concentration of 3mg/g, stir, and form final sea-mussel mucin gel.
4 be separately added into O.lmL Tween-80s, 10mL glycerine, 0.2g benzyl chloride protective embankments ammonia, lg sodium hydrogensulfites, it is well mixed after, add water and supply 100g, form final sea-mussel mucin gel.
After surgery, this appropriate gel coating is taken in the inside and surface of the edge of a knife, it is inside and outside to smear uniform, it is completely covered at whole knife wound, knife edge surfaces changed once above-mentioned gel again at interval of 8-12 hours, the gel can be healed with assist wound, play a part of medical adhesive, effectively facilitate the healing of patient's knife-edge part, and because without suture, wound can very little even without and the gel is all in people's degradable in vivo, and human body is had no side effect, non-toxic and excitant.Adhere to a course for the treatment of（12 days）, blade portion position can be made
Well healed and recovered.Embodiment
1 dissolving sea-mussel mucin dry powder, obtains sea-mussel mucin solution, concentration is 15mg/mL-65mg/mL, and using acetic acid-acetate salt buffer buffer solution, pH is 1-9.
The 2 accurate injection waters for weighing lg carbomers, adding 85mL, stir, and adjusted using sodium hydroxide and 10-120min is placed in pH to 6-7,10 °C -80 °C of environment, form gel.
3 add the 150mg prepared in Shang Shu Walk rapid 1 sea-mussel mucin into Suo Shu Walk rapid 2, make its final concentration of 1.5mg/g, stir, and form final sea-mussel mucin gel.
4 are separately added into O.lmL Tween-80s, 10mL glycerine, O.lg cysteines, O.OOlg chloreresols, O.OOlg rose essential oils, O.Olg silver iodide, after being well mixed, add water and supply 100g, form final sea-mussel mucin gel.
This appropriate gel coating is taken at the position that skin suffers damage because of pernio, smear uniform, whole impaired place is completely covered, above-mentioned gel was changed once again at interval of 8-12 hours, smearing the wettability at position can well be ensured, give patient a kind of comfortable impression, and the microorganism of wound can be killed, give wound one relatively good recovery environment, moreover, the biological characteristics of the good gas permeability of the gel and sea-mussel mucin, the gel in itself can effectively facilitate the recovery of injury.Adhere to a course for the treatment of（7 days）, can well be healed and be recovered with damaged part.Embodiment 8
1 dissolving sea-mussel mucin dry powder, obtains sea-mussel mucin solution, concentration is 10mg/mL-50mg/mL, and using lemon phosphoric acid-phosphate-buffered buffer solution, pH is 1-9.
The 2 accurate injection waters for weighing 1.2g hydroxypropyl methyl celluloses and 2.5g cation guar gums, adding 50mL, stir, pH to 5-7 is adjusted using sodium hydroxide, add water and supply 100g, 10-100min is placed in 10 °C -80 °C of environment, form gel.
During 3 uses, gel is first coated with, then a certain amount of sea-mussel mucin solution is added in gel surface, final sea-mussel mucin gel is formed.
At the external equipment for taking appropriate this gel coating ship; smear uniform; whole equipment is completely covered; and it is that the thickness of gel reaches l-2mm; the gel can effectively protect the equipment of coating; its corrosive speed is reduced, while the normal operation to equipment does not produce any negative impact.Embodiment 9
1 prepares sea-mussel mucin solution, and concentration is 10mg/mL-60mg/mL, using acetic acid-acetate buffer
Liquid, pH is 1-9.
The 2 accurate injection waters for weighing 0.5g hydroxypropyl methyl celluloses and 2.5g cation guar gums, adding 50mL, stir, and adjusted using triethanolamine and 10-120min is placed in pH to 5-7,10 °C -80 °C of environment, form gel.
3 add the 450mg prepared in Shang Shu Walk rapid 1 sea-mussel mucin into Suo Shu Walk rapid 2, make its final concentration of 4.5mg/g, then uniform mixing, the sea-mussel mucin gel of formation.
4 respectively by 10mL sorbierites, 2mL glycerine, 3mL propane diols, 0.02g chloreresols, 0.2g cysteines, 3g PVP-Is, 0.02g silver iodide, O.OOlg rose essential oils, it is added in sea-mussel mucin gel, then uniform mixing, adds water and supplies 100g, then form final sea-mussel mucin gel.
Take this appropriate gel coating in newborn wound, smear uniform, whole injury is completely covered, a gel was changed again at interval of 8-12 hours.The gel can be very good the wettability for keeping smearing position, give patient more comfortable impression.The presence of sea-mussel mucin can significantly reduce the bleeding of wound, effectively facilitate the healing of wound, the presence of PVP-I and silver iodide plays the role of sterilization to wound simultaneously, and sea-mussel mucin can then reduce excitant of these bactericide to wound skin.Adhere to a course for the treatment of（9 days）, damaged part can be made well to be healed and recovered.Industrial applicability
Sea-mussel mucin gel products prepared by the present invention can play the advantage of sea-mussel mucin and gel simultaneously, and obtained sea-mussel mucin gel is easily kept, effect is lasting, action effect is uniform, long action time, good dispersion, adhesive force is strong, stability is good, it is simple, easy to use to prepare, be readily cleaned.
Certainly; the present invention can also have other various embodiments; in the case of without departing substantially from spirit of the invention and its essence; those skilled in the art can make various corresponding changes and deformation according to the present invention, but these corresponding changes and deformation should all belong to the protection domain of the claims in the present invention.
Claims (10)
- Claims1st, a kind of preparation method of sea-mussel mucin gel, it is characterised in that including：The rapid h of Walk obtain or prepared sea-mussel mucin；Walk rapid 2:Obtain or prepare gel-type vehicle；Walk rapid 3:The gel-type vehicle is mixed with the liquid sea-mussel mucin, sea-mussel mucin gel is obtained.2nd, the preparation method of sea-mussel mucin gel according to claim 1, it is characterised in that also include：Walk rapid 4:Gel-type vehicle described in Yu Walk rapid 3 adds auxiliary material after being mixed with the liquid sea-mussel mucin, the auxiliary material is one of nertralizer, solubilizer, NMF, antioxidant, bacteriostatic agent, penetrating agent or aromatic or its any combination.3rd, the preparation method of sea-mussel mucin gel according to claim 1, it is characterized in that, in Suo Shu Walk rapid 1, the sea-mussel mucin is liquid sea-mussel mucin or is the liquid sea-mussel mucin that dry powder state sea-mussel mucin is obtained through the dissolving of acid or neutral solution.4th, the preparation method of sea-mussel mucin gel according to claim 1, it is characterised in that it is to mix gel matrix material with water to prepare gel-type vehicle in Suo Shu Walk rapid 2, and gel-type vehicle is formed after being swelled.5th, the preparation method of sea-mussel mucin gel according to claim 4, it is characterised in that the mass percent that the gel matrix material accounts for the gel-type vehicle is 0. 01-5%.6th, the preparation method of sea-mussel mucin gel according to claim 5, it is characterised in that the mass percent that the gel matrix material accounts for the gel-type vehicle is 0. 08-5%.7th, the preparation method of sea-mussel mucin gel according to claim 1, it is characterised in that acquired gel-type vehicle is gel matrix material in Suo Shu Walk rapid 2.8th, the preparation method of the sea-mussel mucin gel according to claim 4 or 7, characterized in that, the gel matrix material is one of cellulose derivative, carbomer and alginate, tragacanth, gelatin, pectin, carragheen, gellan gum, starch, xanthans, cation guar gum, agar, non-cellulosic polysaccharide, ethene polymers, acrylic resin, polyvinyl alcohol or carbopol or its any combination.9th, the preparation method of sea-mussel mucin gel according to claim 4, it is characterized in that in Yu Walk rapid 3, the hybrid mode of the sea-mussel mucin and the gel-type vehicle is to smear or spray the sea-mussel mucin on the gel-type vehicle to form the sea-mussel mucin gel.10th, the preparation method of sea-mussel mucin gel according to claim 7, it is characterised in that in In Walk rapid 3, the hybrid mode of the sea-mussel mucin and the gel-type vehicle is directly to be swelled the gel rubber material with sea-mussel mucin solution to form the sea-mussel mucin gel.11, the preparation method of sea-mussel mucin gel according to claim 1, it is characterised in that the content of sea-mussel mucin described in the sea-mussel mucin gel is 0. lmg/g-10mg/g.12nd, the preparation method of sea-mussel mucin gel according to claim 2, it is characterised in that the ratio that the auxiliary material accounts for sea-mussel mucin gel gross mass is 0-50%, the pH during auxiliary material addition is the o of 1. 0-9. 013rd, the sea-mussel mucin gel obtained by the preparation method of the sea-mussel mucin gel of claim 1.14th, application of the sea-mussel mucin gel of claim 13 in wound repair product, protecting wound surface product, medical bio adhesive product, medical coating product, industrial coating product, biochemical reagents product, medical product, sterile products, cell culture culture vessel.
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| PCT/CN2013/075918 WO2014186937A1 (en) | 2013-05-20 | 2013-05-20 | Preparation method of mussel adhesive protein gel, mussel adhesive protein gel and use thereof |
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