CN105385565A - Improved bioreactor - Google Patents
Improved bioreactor Download PDFInfo
- Publication number
- CN105385565A CN105385565A CN201510994906.4A CN201510994906A CN105385565A CN 105385565 A CN105385565 A CN 105385565A CN 201510994906 A CN201510994906 A CN 201510994906A CN 105385565 A CN105385565 A CN 105385565A
- Authority
- CN
- China
- Prior art keywords
- tank body
- charging line
- straight sections
- bent portion
- described tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
An improved bioreactor comprises a tank body and a liquid adding pipeline. The liquid adding pipeline is fixed on the upper portion of the tank body, a liquid inlet of the liquid adding pipeline stretches out of the tank body, and a liquid outlet of the liquid adding pipeline is located in the tank body and faces the inner wall of the tank body; two heating modules are symmetrically arranged in the tank body, the tank body is arranged in a base, and the lower portion of the base is provided with wheels. According to the improved bioreactor, culture liquid can flow to the bottom of the tank body along the inner wall of the tank body when the liquid is added, and therefore bubble generation is effectively reduced; in addition, moving is convenient.
Description
Technical field
The present invention relates to a kind of modified version bio-reactor, belong to biological technical field.
Background technology
The charging line of bio-reactor is for supplementing nutrient solution, inoculating cell etc. in the tank body of bio-reactor.In macro-organism reactor, the volume of required cell culture fluid or required inoculating cell suspension is comparatively large, so need to apply the larger pipeline of internal diameter as DN25 or DN40 etc.The charging line of traditional bio-reactor adopts a pipeline directly to be passed into tank base by tank body top usually, if according to this traditional liquid feeding mode, there are the following problems for possibility: 1) tank interior can increase the position being difficult to clean, and may form cleaning dead angle; 2) after liquid feeding, the inside of charging line may retain part debris and form dead band, effectively cannot merge with the cell outside charging line; 3) because tank body is longer, if charging line directly passes into tank base from tank body top, comparatively difficulty is installed.In addition, if directly add cell culture fluid or inoculating cell suspension from tank body top, more, larger bubble can be produced again, and the shearing force produced during bubbles burst to cell damage, even can may cause necrocytosis.
Summary of the invention
The object of this invention is to provide a kind of modified version bio-reactor, by arranging the charging line of improvement, realize adherent liquid feeding mode, solve in prior art and adopt when carrying out liquid feeding by the tank body top charging line directly passed into tank base that existing tank interior exists clean dead angle, charging line inside exists debris, pipelining difficulty and to problems such as cell damage.Technical scheme provided by the present invention is: a kind of modified version bio-reactor, comprise tank body and charging line, described charging line is fixed on the top of described tank body, and the fluid inlet of described charging line stretches out outside described tank body, the liquid outlet of described charging line is positioned at described tank body, and the liquid outlet of described charging line is towards the inwall of described tank body, is also arranged with two heating modules in described tank body, described tank body is placed in base, and described base bottom is provided with wheel.
As preferably, described charging line comprises: straight sections, is fixed on the side wall upper part of described tank body, and the fluid inlet of the first end of described straight sections stretches out outside described tank body, and the second end of described straight sections stretches in described tank body; Bent portion, the first end of described bent portion docks with the second end of described straight sections, and the liquid outlet of the second end of described bent portion is towards the inwall of described tank body.
As preferably, described straight sections is horizontally placed on the side wall upper part of described tank body; Described bent portion is semicircular bend pipe structure.
As preferably, described bent portion and described straight sections connect as one structure.
Compared with prior art, beneficial effect of the present invention is as follows: nutrient solution can enter in tank body by charging line, because the liquid outlet of charging line is towards inner tank wall, during liquid feeding, nutrient solution along inner tank wall to tank base stream, can effectively reduce the generation of bubble; In addition, modified version bio-reactor of the present invention is convenient mobile.
Accompanying drawing explanation
Fig. 1 represents the structural representation of modified version bio-reactor of the present invention.
Embodiment
Be described principle of the present invention and feature below in conjunction with accompanying drawing, example, only for explaining the present invention, is not intended to limit scope of the present invention.
In prior art, the charging line of modified version bio-reactor normally adopts a pipeline directly to stretch to tank base by tank body top, such structure can exist when liquid feeding produce bubble many and to cell produce injury, can be formed in clean dead angle, pipeline can retain debris and install difficulty etc. problem.
Modified version bio-reactor of the present invention, can realize adherent liquid feeding mode, produces bubble when reducing liquid feeding.Refer to Fig. 1, modified version bio-reactor of the present invention comprises tank body 2 and charging line 1, wherein, charging line 1 is fixed on the top of tank body 2, and the fluid inlet 1a of charging line 1 stretches out outside tank body 2, the liquid outlet 1b of charging line 1 is positioned at tank body 2, and the liquid outlet 1b of charging line 1 is towards the inwall of tank body 2.
Adopt the modified version bio-reactor of this structure, nutrient solution can enter in tank body 2 by charging line 1, liquid outlet 1b due to charging line 1 is the inwall facing toward tank body 2, therefore, during liquid feeding, nutrient solution can flow bottom tank body 2 along tank body 2 inwall, and this adherent liquid feeding mode can reduce the generation of bubble effectively, thus avoids cell to be subject to the injury of the shearing force that bubbles burst produces.
In addition, in the present invention, be also arranged with two heating modules 3, can regulate the temperature in tank body 2 in described tank body 2, described tank body 1 is placed in base 4, and described base 4 bottom is provided with wheel 5, convenient mobile.In the present invention, described charging line comprises: straight sections, is fixed on the side wall upper part of described tank body, and the fluid inlet of the first end of described straight sections stretches out outside described tank body, and the second end of described straight sections stretches in described tank body; Bent portion, the first end of described bent portion docks with the second end of described straight sections, and the liquid outlet of the second end of described bent portion is towards the inwall of described tank body.
In the preferred embodiment of the present invention, the structure that bent portion and straight sections can be integrated, structure is simple, easy processing and manufacturing.
Protection scope of the present invention includes but not limited to above embodiment, and protection scope of the present invention is as the criterion with claims, and any replacement that those skilled in the art will find apparent that, distortion, improvement made this technology all falls into protection scope of the present invention.
Claims (4)
1. modified version bio-reactor, comprise tank body and charging line, it is characterized in that, described charging line is fixed on the top of described tank body, and the fluid inlet of described charging line stretches out outside described tank body, and the liquid outlet of described charging line is positioned at described tank body, and the liquid outlet of described charging line is towards the inwall of described tank body, also be arranged with two heating modules in described tank body, described tank body is placed in base, and described base bottom is provided with wheel.
2. modified version bio-reactor according to claim 1, it is characterized in that, described charging line comprises: straight sections, is fixed on the side wall upper part of described tank body, and the fluid inlet of the first end of described straight sections stretches out outside described tank body, the second end of described straight sections stretches in described tank body; Bent portion, the first end of described bent portion docks with the second end of described straight sections, and the liquid outlet of the second end of described bent portion is towards the inwall of described tank body.
3. modified version bio-reactor according to claim 2, is characterized in that, described straight sections is horizontally placed on the side wall upper part of described tank body, and described bent portion is semicircular bend pipe structure.
4. modified version bio-reactor according to claim 2, is characterized in that, described bent portion and described straight sections connect as one structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510994906.4A CN105385565A (en) | 2015-12-28 | 2015-12-28 | Improved bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510994906.4A CN105385565A (en) | 2015-12-28 | 2015-12-28 | Improved bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105385565A true CN105385565A (en) | 2016-03-09 |
Family
ID=55418352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510994906.4A Pending CN105385565A (en) | 2015-12-28 | 2015-12-28 | Improved bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105385565A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652299A (en) * | 2018-11-15 | 2019-04-19 | 上海量能生物科技有限公司 | Bioreactor with resistance to movement function |
-
2015
- 2015-12-28 CN CN201510994906.4A patent/CN105385565A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109652299A (en) * | 2018-11-15 | 2019-04-19 | 上海量能生物科技有限公司 | Bioreactor with resistance to movement function |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160309 |
|
| WD01 | Invention patent application deemed withdrawn after publication |