CN105368707B - DNA sequencer with CCD camera adjusted in single-dimensional mode - Google Patents
DNA sequencer with CCD camera adjusted in single-dimensional mode Download PDFInfo
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- CN105368707B CN105368707B CN201510716484.4A CN201510716484A CN105368707B CN 105368707 B CN105368707 B CN 105368707B CN 201510716484 A CN201510716484 A CN 201510716484A CN 105368707 B CN105368707 B CN 105368707B
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- 238000006243 chemical reaction Methods 0.000 claims abstract description 144
- 230000033001 locomotion Effects 0.000 claims abstract description 32
- 238000009434 installation Methods 0.000 claims description 24
- 238000001514 detection method Methods 0.000 claims description 18
- 238000013016 damping Methods 0.000 claims description 12
- 238000005859 coupling reaction Methods 0.000 claims description 4
- 230000008878 coupling Effects 0.000 claims description 3
- 238000010168 coupling process Methods 0.000 claims description 3
- 230000003014 reinforcing effect Effects 0.000 claims description 3
- 230000004044 response Effects 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 description 24
- 238000010586 diagram Methods 0.000 description 21
- 238000012856 packing Methods 0.000 description 9
- 230000001939 inductive effect Effects 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 5
- 238000007789 sealing Methods 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 238000001712 DNA sequencing Methods 0.000 description 3
- 210000005056 cell body Anatomy 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
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- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- 238000003754 machining Methods 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
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- 239000000376 reactant Substances 0.000 description 1
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- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The invention relates to a DNA sequencer with a CCD camera adjusted in a single-dimensional mode. The DNA sequencer with the CCD camera adjusted in the single-dimensional mode comprises a reaction bin assembly, the CCD camera capable of moving front and back relative to the reaction bin assembly to take photos of a reaction bin, a motion platform assembly driving the CCD camera to conduct linear reciprocating motion, and a platform supporting assembly connected with the motion platform assembly, wherein the CCD camera and the reaction bin assembly are arranged in the same horizontal direction. The CCD camera of the DNA sequencer with the CCD camera adjusted in the single-dimensional mode can conduct one-dimensional motion in the direction of the reaction bin assembly, space is saved for the sequencer, then the reaction bin assembly can rotate in the vertical plane, and the overall structure is stable.
Description
Technical field
The present invention relates to DNA sequencing field, more particularly to a kind of DNA sequencer of CCD camera single dimension adjustment.
Background technology
In DNA sequencing technology field, in sequencing procedure, there is chemical reaction on sequence testing chip, produce visible ray, pass through
Optical signal produced by CCD (Charge Couple Device) cameras capture sequencing reaction, you can the sequencing required for obtaining
Information.
In existing sequenator, disclose multiple reaction warehouses and be arranged side by side and there is planar motion accordingly
CCD camera, CCD camera is except can be in the direction upper feeding of reaction warehouse, additionally it is possible on the direction vertical with multiple reaction warehouses
Feeding.And CCD camera only arranges one, in test process, constantly the position of adjustment CCD camera, adjusts the ginseng of CCD camera
Number, the fussy degree of data acquisition and processing (DAP) are high, and during constantly adjustment CCD camera, the stability of sequenator can not
Ensure, therefore, the CCD camera of single dimension can more meet the requirement of the precision and stability of DNA sequencing.
In view of drawbacks described above, creator of the present invention passes through prolonged research and practice obtains this creation finally.
The content of the invention
It is an object of the invention to provide a kind of DNA sequencer of CCD camera single dimension adjustment, to overcome above-mentioned technology
Defect.
For achieving the above object, the present invention provides a kind of DNA sequencer of CCD camera single dimension adjustment, and which includes reaction
Storehouse component, is arranged on same level direction with reaction warehouse component and energy relative response storehouse component moves forward and backward, reaction warehouse is carried out
The CCD camera taken pictures, drives the motion platform component of described CCD camera linear reciprocation movement, and with motion platform component
The platform component of connection；
Described motion platform component includes the lead screw guide rails component that motor is connected with motor output shaft, supports leading screw
The support base of component, detection components；Nut is connected with leading screw, nut is arranged on nut mounting seat nut mounting seat and is arranged on
On the slide block of guide rail contact, and camera movement is driven on slide block；
Described reaction warehouse component is arranged on the door frame on front side of platform component, is arranged on the top in the outside of door frame
There is turnable reaction warehouse component, turnable basement being connected with the lower end that may be reversed reaction warehouse component, turnable basement is fixed on
The middle part of door frame, may be reversed reaction warehouse component can rotate around described turnable basement, to take out or be put into reaction chip；
Described platform component includes vibration damper plate and is arranged on the first damping wing plate and second of vibration damper plate both sides
Damping wing plate, is respectively arranged with dampening assembly in the lower end of two damping wing plates；After installation is complete, platform component is in recessed
Mouth shape, reaction warehouse component are arranged on vibration damper plate, and two ends are contacted with two vibration damper plates respectively.
Further, described damping wing plate includes gripper shoe, and which has certain height, supports described reaction warehouse group
Part；The middle setting of the integrally formed upper head plate of gripper shoe has the mounting groove of motion control unit, is mounted therein with motor control
The circuit board of unit and various circuits；
It is reinforcing plate in the lower end of mounting groove, to open up mounting groove.
Further, the output shaft of motor is connected by shaft coupling with leading screw, and motor is provided with support with leading screw junction
Seat, is provided with bearing and axial positioning sleeve in the end of leading screw；
The upper end of described nut mounting seat is connected with connecting plate, and the upper end of connecting plate is connected with camera mounting seat；In axle
Hold end and bearing friction top is set.
Further, the upper end of described nut mounting seat is provided with connecting plate installing hole, and mid portion is provided with through hole,
To through leading screw；The surrounding of through hole is fastening thread hole, to install with nut.
Further, described support base includes leading screw installation sleeve, and positioning step is arranged in which；The end of installation sleeve
Installing plate is connected with, to be connected with motor end face.
Further, described detection components include detection plate fixed seat, and which is arranged on the sidepiece of connecting plate, and detection plate is solid
The end of reservation is connected with position detection plate；Position sensor mounting seat is installed in the sidepiece of base, is pacified in position sensor
The upper end of dress seat arranges contiguous block, and position sensor is fixed in contiguous block upper end.
Further, described position sensor mounting seat includes being arranged on the screwed hole of side, in the centre of mounting seat
For mounting groove, to installation site sensor；Slide recess is provided between sidepiece baffle plate and bottom surface.
Beneficial effects of the present invention are compared with prior art：The DNA sequencer of the CCD camera single dimension adjustment of the present invention
CCD camera can carry out motion in one dimension in the direction with reaction warehouse component, be that sequenator saves space so that reaction warehouse group
Part can be rotated in perpendicular, and overall structure is stable.
Description of the drawings
Fig. 1 is the overall structure diagram of the DNA sequencer reaction record subsystem of the present invention；
Fig. 2 is the first dimensional structure diagram of the reaction warehouse component of the present invention；
Fig. 3 is the second dimensional structure diagram of the reaction warehouse component of the present invention；
Fig. 4 is the positive structure diagram of the reaction warehouse component of the present invention；
Fig. 5 a are the dimensional structure diagram of the turnable reaction warehouse component of the present invention；
Fig. 5 b are the first dimensional structure diagram of the turnable warehouse of the present invention；
Fig. 5 c are the second dimensional structure diagram of the turnable warehouse of the present invention；
Fig. 5 d are the first dimensional structure diagram of the locating piece of the present invention；
Fig. 5 e are the second dimensional structure diagram of the locating piece of the present invention；
Fig. 6 a are the structural representation of the electronic lock of the present invention；
Fig. 6 b are the structural representation of the electronics lock of the present invention；
Fig. 6 c are the cross section structure diagram of the electronic lock of the present invention；
Fig. 7 is the cross section structure diagram of the turnable reaction warehouse component along first lug of the present invention；
Fig. 8 is the 3rd dimensional structure diagram of the turnable warehouse of the present invention；
Fig. 9 a are the structural representation of the reaction chip component of the present invention；
Fig. 9 b are the structural representation of the reaction chip mounting seat of the present invention；
Fig. 9 c are the structural representation of the medial surface of the reaction warehouse base of the present invention；
Fig. 9 d are the structural representation of the lateral surface of the reaction warehouse base of the present invention；
Figure 10 is the cross section structure diagram along liquid inlet and outlet of the reaction chip component of the present invention；
Figure 11 a are the cross section structure diagram of the turnable reaction warehouse component along self-adaptative adjustment part of the present invention；
Figure 11 b are the cross section structure diagram of the turnable reaction warehouse component along guide post of the present invention；
Figure 12 is A-A of the turnable reaction warehouse component of the present invention in Fig. 4 to cross section structure diagram；
Figure 13 a are the cross section structure diagram of the back shroud of the present invention；
Figure 13 b are the dimensional structure diagram of the bonnet board component of the present invention；
Figure 13 c are the dimensional structure diagram of the back shroud of the present invention；
Figure 14 is the structural representation of the platform component of the present invention；
Figure 15 is the structural representation of the vibration damper plate of the present invention；
Figure 16 is the structural representation of the motion platform component of the present invention；
Figure 17 is the cross section structure diagram of the motion platform component of the present invention；
Figure 18 a are the structural representation of the nut mounting seat of the present invention；
Figure 18 b are the structural representation of the motor with the support base of leading screw junction of the present invention；
Structural representations of Figure 18 c for the detection components in the embodiment of the present invention；
Figure 18 d are the structural representation of position sensor mounting seat of the present invention.
Specific embodiment
It is below in conjunction with accompanying drawing, above-mentioned to the present invention to be described in more detail with other technical characteristic and advantage.
Refer to shown in Fig. 1, which is the overall structure diagram of the DNA sequencer reaction record subsystem of the present invention, this
In inventive embodiments reaction record subsystem include reaction warehouse component 12, motion platform component 14, motion control unit 15,
CCD camera 101 and platform support component 13, wherein,
Described reaction warehouse component 12, arranges reaction chip component in which, in the presence of control system, reactant liquor is entered
Chemical reaction is carried out in reaction chip, visible ray is produced；Described motion platform component 14, CCD camera is fed into suitably by which
Picture-taking position, the visible optical information to producing in reaction chip carries out collection of taking pictures；Described platform component 13, its
The described reaction warehouse component 12 of support, motion platform component 14；Described motion control unit, which is to CCD camera and reaction core
The operation real-time control of piece.
In embodiments of the present invention, described reaction chip can be installed, be fixed and be adjusted to described reaction warehouse component 12,
Ensure that reaction chip is reacted in stable environment, also, reaction chip can conveniently take out and lock.
Refer to shown in Fig. 2-4, described reaction warehouse component 12 includes being arranged on described platform component 13
The door frame 121 being vertically arranged, is provided with turnable reaction warehouse component 124 on the top in the outside of door frame 121, may be reversed reaction
The lower end of storehouse component 124 is connected with turnable basement 122, and turnable basement 122 is fixed on the middle part of door frame 121, may be reversed anti-
Answer storehouse component 124 rotate around described turnable basement 122, to take out or be put into reaction chip.
Electronics lock set 125 is additionally provided with the left of the upper end of described door frame 121, only in electronics lock set 125
During opening, turnable reaction warehouse component 124 can be rotated, reaction chip is operated.In embodiments of the present invention, if
Door frame 121 is equipped with, reaction chip and dependency structure is supported, is substantially increased the stability of system.
Turnable reaction warehouse component 124 includes may be reversed warehouse, is provided with the mid portion of described turnable warehouse
Back shroud 1242, to encapsulate Reagent Tube；Bottom is provided with the first rotating part, which includes the first accommodating chamber 1243, to hold
Receive turnable basement 122, the first accommodating chamber 1243 is arranged on the centre position of bottom；It is to be symmetrical arranged in the first accommodating chamber both sides
First lug 1244, have through hole 1246 in the middle setting of first lug 1244；To connect with described turnable basement 122
Connect.Turnable basement is rotated in the first accommodating chamber 1243 relative to turnable warehouse, with higher stability, while ensureing examination
Agent and the transmission of data are unimpeded.
Refer to shown in Fig. 5 a-5c, reaction chip component, phase are installed on the inside of the centre of described turnable warehouse
Answer, have the first cavity 1785 for accommodating reagent pipeline in the middle setting that may be reversed warehouse, in the bottom of the first cavity 1785
Be provided with first boss 1784, first boss 1784 to carry the support member of described reaction chip component, in first boss
1784 are provided with four guide post installing holes 1786, and guide post installing hole 1786 is through two ends, the reaction chip that may be reversed warehouse
Component and support member, reaction chip component is connected with turnable warehouse；Arrange in the upper end of described first boss 1784
There is second boss 1783, second boss 1784 is to position described support member；Arrange in described 1784 upper end of second boss
There is locating surface 1782, locking screw nail 1787 is provided with locating surface 1782.It is anti-described in above-mentioned first cavity body structure positioning
Chip assembly is answered, also, reaction chip component is in normal work, it is impossible to be enough to rotate, be merely able to move along guide post.
1788 He of Reagent Tube installing hole is provided with the upper surface of the first accommodating chamber 1243 of described turnable warehouse
Electric wire installing hole, is provided with pipe clamp 03 in Reagent Tube installing hole 1788, and passing through in pipe clamp 03 has Reagent Tube 02 and electric wire 01.
The dead bolt that the dead bolt 1252 in electronics lock set 125 is additionally provided with the sidepiece of described turnable warehouse is installed
Hole；In the presence of locking member, may be reversed warehouse and be fixed together with door frame 121.
Incorporated by reference to shown in Fig. 6 a and 6b, the electronics lock set 125 of the present invention includes electronics lock and inductive switch 1251, institute
The inductive switch stated is arranged on electronics lock, and electronics lock is arranged on the sidepiece of turnable warehouse, in embodiments of the present invention,
Electronics lock includes sidepiece installing plate 1843, and installing hole 1844 is offered on sidepiece installing plate 1483；Electronic lock is made by peace
Dress hole 1844 and mounting screw are fixed on turnable warehouse.It is provided with described 1483 side of sidepiece installing plate symmetrical
First framework 1841 and second framework 1842, leave the sky for installing inductive switch 1251 in the centre of the first framework and second framework
Between.
In embodiments of the present invention, described inductive switch 1251 includes the framework 1255 for being arranged on outside, framework 1255
One side on be provided with connection screw thread hole 1256, which is fixed by attachment screw 1254 with described electronics lock；Described
Attachment screw 1254 passes through lug 1258 and the first framework 1841, second framework 1842 on side, to increase electronics lock
Bonding strength.Control circuit 1252 is also associated with described inductive switch 1251, to carry out to inductive switch 1251
Control.
Refer to shown in Fig. 6 c, described electronics lock set 125 is arranged on the sidepiece that may be reversed warehouse and door frame 121, lock
Tongue 1253 is entered after stretching out and is arranged in the dead bolt hole 1247 of turnable warehouse sidepiece, realizes locking；When dead bolt 1253 is in electricity thorn
In the presence of swashing, when retracting from dead bolt hole 1247, described turnable warehouse can be rotated.
The upper end in the outside of described turnable warehouse is provided with pressing piece 1241, is only being pressed by pressing piece 1241
Pressure is arranged can make described turnable warehouse rotation.Refer to shown in Figure 12, in embodiments of the present invention, described pressing
1241 one end of part are provided with the structure that opening 1730, one end is provided with hook, and described hook is connected with the upper end-stopping plate of door frame
Connect, when pressing piece 1241 is subject to downward active force, hook is departed from door frame baffle plate, and may be reversed reaction warehouse body can be with respect to door
Frame rotates.
In embodiments of the present invention, described pressing piece 1241 is arranged in a positioning block 1762, under locating piece 1762
End is provided with spring 1761, and spring is arranged in spring base 1763, and spring base 1763 is threadably mounted at and may be reversed reaction warehouse
In the spring base hole 1781 of 1785 upper surface of intermediate throughholes.
Incorporated by reference to shown in Fig. 5 b-5d, locating piece 1762 is arranged on the locating piece installation cavity that may be reversed reaction warehouse upper end
In 1791, positioning step 1790 is provided with cavity.Locating piece 1762 in the embodiment of the present invention includes 1794 He of through hole
Lower through-hole 1797, one of both pass through described pressing piece 1241；The side of locating piece arrange locating and machining hole 1796 and
Installing hole 1795.The lower end of the through hole for installing pressing piece 1241 is provided with spring eye, spring is by the hole and pressing piece 1241
Bottom contact.
The embodiment of the present invention arranges electronics lock set and pressing piece, carries out two to may be reversed reaction warehouse component and door frame
Secondary locking, is respectively adopted automatically controlled and frame for movement mode, it is ensured that reaction chip is run in security context, it is to avoid maloperation.
Refer to shown in Fig. 2 and 7, the turnable basement in the embodiment of the present invention 122 has two the second lugs 1221
Connect base, the second lug 1221 is symmetricly set on a side 1223 of base, and through hole 1224 is provided with the middle of which,
It is co-axially mounted with the through hole 1246 of the middle setting of the first lug 1244 on described turnable warehouse, also, the second lug
1221 inner sides for being arranged on first lug 1244.
Incorporated by reference to shown in Fig. 2, in embodiments of the present invention, door frame 121 has the structure of sidepiece baffle plate 1211 for one side,
Which is integrally formed, and the both sides of described turnable basement 122 are arranged in sidepiece baffle plate 1211 and are contacted with sidepiece baffle plate 1211
Positioning；Described first lug 1224 after being co-axially mounted with the second lug 1221, the upper termination of end and turnable basement 122
Touch, also, may be reversed the sidepiece of warehouse and contact with 1211 medial surface of sidepiece baffle plate of door frame 121, it is ensured that turnable warehouse with can
Upset base 122 is located just in sidepiece baffle plate 1211.
The receiving hole passed through for reaction chip component is additionally provided with door frame 121.The use of door frame, be not only able to so that
The Stability Analysis of Structures of reaction chip component, and, also so that may be reversed warehouse and turnable basement compact conformation, it is easy to operation；CCD
Camera is more accurate with the positioning of reaction chip.
The bottom of door frame 121 is provided with the first installing hole 1210, to be connected with motion platform component 14；In door
The second installing hole being connected with turnable basement sidepiece is additionally provided with the middle part of frame.
Incorporated by reference to shown in Fig. 7, in embodiments of the present invention, in order to save structure space and ensure the steady of reagent delivery
It is fixed, reagent is provided with the through hole of the through hole 1246 and the second lug 1221 of the middle setting of described first lug 1244
The first fixation kit of pipe, which includes being arranged on pipe joint 1265 in the lug of left side, the first packing ring 1264, the first rotary shaft
1261, and the reagent pipe joint 1266 on the right side of being arranged in lug, second packing ring 1267, the second rotary shaft 1269, wherein, institute
The first packing ring 1264 stated and second packing ring 1267 are arranged between turnable warehouse and turnable basement, in the present embodiment,
Diameter of the diameter of the through hole 1246 of the middle setting of described first lug 1244 less than the through hole of the second lug 1221,
Both medial end forms the positioning rib to packing ring positioning, and after two packing rings are installed, inside diameter of gasket is just with second
The diameter of the through hole of lug 1221 is identical.Positioning rib and packing ring are set in embodiments of the present invention, it is ensured that may be reversed storehouse
Gapless between body and turnable basement, both good stabilities after combining, will not rock because there is gap, also,
Packing ring increased both contacts area so that stress between the two is reduced, extension device service life.
The first rotation is installed on the inside of the inner side of the through hole 1246 of the middle setting of first lug 1244 and packing ring
Axle 1261 and the second rotary shaft 1269, the structure of two rotary shafts are identical, are the quill shaft with positioning convex ring；In rotary shaft
One end arrange positioning convex ring, be provided with connection screw thread hole, to the lug connection portion with described turnable warehouse
1263 are connected, and accordingly, the positioning recess for positioning the positioning convex ring are provided with the end of described through hole 1246.
The centre at the both ends of rotary shaft 1269 be recess, inner side recess to described pipe joint 1265, reagent pipe joint
1266 install positioning.Live wire and Reagent Tube 02 are respectively mounted in pipe joint 1265, reagent pipe joint 1266, its difference
Lead in reaction chip component.As the component that mutually can be overturn is arranged in the embodiment of the present invention, in upset, to avoid to pipe
Part is impacted, and installs Reagent Tube in through-holes, substantially increases the security performance of Reagent Tube.
Refer to shown in Fig. 8, in embodiments of the present invention, described reaction chip component is arranged on described turnable storehouse
The inner side of body.The observation portion 1724 contacted with CCD camera is provided with the inside of reaction chip component, is provided with transparent
Baffle plate, to take pictures to course of reaction.
Refer to shown in Fig. 9 a, the reaction chip component of the present invention includes reaction chip mounting seat 127, reaction warehouse base group
Part 126 and the shading positioning component 128 reaction warehouse base assembly 126 and reaction chip mounting seat 127 locked, is opened；For
Easily reaction chip can be taken out and be closed, reaction chip mounting seat 127 can be relative with reaction warehouse base assembly 126
Rotation.
Refer to shown in Fig. 9 b, which is the structural representation of the reaction chip mounting seat of the present invention, is installed in reaction chip
The mid portion of seat 127 is provided with reaction chip, and cover plate 1277 is provided with reaction chip；Install in the both sides of reaction chip
There is chute pressing plate 1279, to be fixed to reaction chip.A side portion of reaction chip mounting seat 127 is provided with installation
Seat supports component, which is in taking-up or chip placement, to support described reaction chip mounting seat；Which includes torsional-spring supported portion
1273, it is arranged in mounting seat, to support described torsion spring, described torsional-spring supported portion 1273 is one with protruding end 1274
Recess, in the described reaction chip mounting seat 127 of rotation, protruding end 1274 is contacted with 1261 at first, in the present embodiment,
Described protruding end 1274 is arranged on the middle part of recess and the edge of chip installation position；Using the structure of mounting seat itself
Reaction chip mounting seat is supported, better reliability, it is ensured that chip can be safely removed and be placed.
Described mounting seat support component also include torsion spring 1272, and torsion spring 1272 connect and be arranged on the drawing at torsion spring two ends
Mandrel 1271, described tension axle 1271 are arranged in lobe 1275, when reaction chip mounting seat is started, described torsion spring
1272 support mounting seat, and finally by the positioning of protruding end 1274, realize supporting.
The first rotary shaft hole 1276 is additionally provided with the end of described lobe 1275, to reaction warehouse base 1261
Connection.Locating slot 1278 is additionally provided with the sidepiece of reaction chip mounting seat.
Refer to shown in Fig. 9 c, which is the structural representation of the medial surface of the reaction warehouse base of the present invention, in reaction warehouse
Reaction cell body 1266 is provided with medial surface, reaction tank 1267 is set in 1266 mid portion of reaction cell body, in reaction tank 1267
Surrounding 1706 be provided with sealing ring, prevent reaction reagent from leaking.One end of reaction warehouse base 1261 is provided with raised line 1705,
To position with the cover plate after installation.The other end is provided with the torsional-spring supported structure corresponding with reaction chip mounting seat, its
Including symmetrically arranged two protruding ends 1268, the second rotary shaft hole 1269 is provided with thereon, itself and the first rotary shaft hole
1276 is coaxial, and rotary shaft sequentially passes through holes；Region 1260 and positioning region for torsion spring activity are additionally provided with the end
1707, it is provided with the region to the screwed hole 1712 by reaction Hatch Board is installed.
Refer to shown in Fig. 9 d, which is the structural representation of the lateral surface of the reaction warehouse base of the present invention, at reaction warehouse bottom
The mid portion of the lateral surface of seat is provided with temperature control component, and the mid portion of reaction warehouse base is provided with temperature sensor installation
Groove 1262, is provided with heating wire mounting groove 1704 in the bottom of mounting groove 1262, places heating wire and temperature sensor respectively.
A positioning boss is additionally provided with mounting groove, is positioned when which is to install with temperature control component cover plate 1263.
The surrounding of temperature sensor mounting groove 1262 is provided with four guide post installing holes 1742, is provided with upper and lower sides
Self adaptation spring mounting hole 1741；It is also equipped with being oriented to rod aperture 1701 on cover plate 1263.
Shading positioning component mounting groove 1265 is additionally provided with reaction warehouse base, to install described shading positioning group
Part 128, is provided with installation base 1744 in which.
Refer to shown in Figure 10 and 11a, the middle part of reaction warehouse base is provided with electrical heating wire 1754, which is arranged on
In the heating wire mounting groove 1704 of base；Temperature sensing is provided with the temperature sensor mounting groove 1262 on electrical heating wire top
Device 1720 and control assembly, to detect that the temperature of reaction tank the threshold value according to setting are adjusted；In temperature sensor
1720 and control assembly 1753 also pour and be inoculated with sealing member 1263, which is encapsulating resin.
Reagent Tube is respectively arranged with the two ends of reaction tank screwed hole 1721 is installed, to be connected with Reagent Tube；In reagent
Pipe installs the two ends that the end of screwed hole 1721 is provided with inlet 1723 and liquid outlet 1713, liquid inlet and outlet and reaction tank
Communicate, to cycle detection reagent.
The avris of the interior grooves of reaction tank is provided with sealing ring 1713.It is provided with the centre of reaction chip mounting seat
Reaction chip 04, reagent flow through reaction chip and react.
In embodiments of the present invention, Reagent Tube 02 is arranged on and can be turned over by the Reagent Tube installing hole 1788 of turnable warehouse
The middle setting of roll-over body has the first cavity 1785 for accommodating reagent pipeline, and final coupled reaction cell body, reagent cartridge pass through each
The sealing of cavity is planted, stability is high, is hardly damaged.
Back shroud 1242 is fixed with rear side of reaction warehouse, the middle setting of back shroud 1242 has self-adaptative adjustment part
1751, refer to shown in Figure 13 a-13c, there are two rows that raised line 1809 is installed in the inner side middle setting of back shroud 1242, installing
The middle setting of raised line 1809 has the first installation base 1812 and the second installation base 1811, and screw thread is offered on installation base
Hole 1802, self-adaptative adjustment part 1751 are arranged in the screwed hole 1802.
In embodiments of the present invention, described self-adaptative adjustment part 1751 is the end of thread with the connection end of back shroud, and anti-
The connection end for answering storehouse component is the polished rod 1805 for being provided with spring 1806, is provided with nut 1804 between the ends and is positioned,
After installation is complete, nut 1804 is close to installation base.
It is passage 1803 between two described raised lines 1809, enables to Reagent Tube and pass through；Also set on back shroud
It is equipped with the reinforcement gusset 1812 vertical with raised line 1809；In raised line 1809 and 1810 bit plane of upper surface of boss；In plate below
Peripheral framework 1807 centre and corner be provided with trip bolt hole 1801, to connect with described turnable reaction warehouse
Connect fixation.
Above-mentioned bonnet board component, is not only able to carry out face seal to reaction warehouse component, additionally it is possible to installs fixed self adaptation and adjusts
One integral piece, simple structure.
In embodiments of the present invention, may be reversed between reaction warehouse and reaction warehouse base 126 and be provided with self-adaptative adjustment part
1751, refer to shown in Figure 12, the end spring of self-adaptative adjustment part 1751 and the end contact of reaction warehouse base；Using
When CCD camera feeds, when camera is with reaction chip component touch, when camera position is adjusted, movable side can be adopted
Formula is adjusted, and now, self-adaptative adjustment part can change the position of reaction chip component according to the change in location of camera, it is ensured that have
Higher accuracy of detection, while, it is to avoid system is caused to damage.
In embodiments of the present invention, in order to ensure that may be reversed reaction warehouse component coaxially can be moved with reaction chip component,
It is convenient to adjust, guide post 1758 is additionally provided between the two, refers to shown in Figure 11 b, guide post is set on reaction warehouse base
Hole 1742, arranges guide post installing hole 1786 on it may be reversed warehouse, and guide post 1742 sequentially passes through holes.Described guide post
1758 include reaction warehouse locating flange 1757, to position with guiding rod aperture 1742；In the other end by nut 1755 and guiding
The end face positioning of bar installing hole 1786, is additionally provided with pad 1756 between.
In embodiments of the present invention, in order to test to the diverse location of reaction chip, employing can change survey
The sealing ring 1713 of examination area.
The platform component of the embodiment of the present invention includes vibration damper plate 1497 and is arranged on the of 1497 both sides of vibration damper plate
One damping wing plate 1492 and the second damping wing plate 1491, are respectively arranged with dampening assembly 1493 in the lower end of two damping wing plates.
After installation is complete, platform component is in indentations, and reaction warehouse component 12 is arranged on vibration damper plate 1497, and two ends are respectively with two
Individual vibration damper plate contact.
Damping wing plate includes gripper shoe 1497, and which has certain height, supports described reaction warehouse component；Gripper shoe
The middle setting of 1497 integrally formed upper head plates 1495 has the mounting groove 1492 of motion control unit 15, is mounted therein with motion
The circuit board of control unit 15 and various circuits；It is reinforcing plate 1496 in the lower end of mounting groove 1492, to open up mounting groove
1492.The platform component of the embodiment of the present invention can either be symmetrical arranged the stability of guarantee system, can arrange control again
Component, saves space, compact conformation.
Motion platform component 14 and CCD camera 101 are additionally provided with platform component 13, are referred to shown in Figure 16,
Described motion platform component 14 includes motor 136, be connected with motor output shaft lead screw guide rails component, support lead screw assembly
Support base and detection components, camera support component.
Refer to shown in Figure 17, the output shaft of motor 136 is connected by shaft coupling 1377 with leading screw 137, motor and leading screw
Junction is provided with support base 1361, and the end of leading screw is provided with bearing 1371 and axial positioning sleeve 1376.On leading screw
Nut 1374 is connected with, nut is arranged in nut mounting seat 1375；The upper end of nut mounting seat 1375 is connected with connecting plate 1012
Connect, the upper end of connecting plate 1012 is connected with camera mounting seat 1011；In bearing end, bearing friction top 1372 is set.
Described nut mounting seat 1375 is on the slide block 1012 contacted with guide rail 132, and phase is driven on slide block
Machine is moved.
Figure 18 a are referred to, which is the structural representation of nut mounting seat, its upper end is provided with connecting plate installing hole 1401,
Mid portion is provided with through hole 1402, to through leading screw；The surrounding of through hole 1402 be fastening thread hole 1403, to nut
Install.
Refer to shown in Figure 18 b, which is the structural representation of motor and the support base of leading screw junction, which includes that leading screw is pacified
Sleeved 1363, positioning step 1364 is set in which；The end of installation sleeve is connected with installing plate 1362, to motor end face
Connection.
Refer to shown in Figure 18 c, the detection components in the embodiment of the present invention include detection plate fixed seat 1353, and which is arranged on
The sidepiece of connecting plate 1012, the end of detection plate fixed seat 1353 are connected with position detection plate 1352；Install in the sidepiece of base
Position sensor mounting seat 1351, in position, the upper end of sensor installation seat 1351 arranges contiguous block 1356, contiguous block upper end
Fixed position sensor 135.
Refer to shown in Figure 18 d, which is the structural representation of position sensor mounting seat of the present invention, and which includes being arranged on side
The screwed hole 1415 in face 1411, is mounting groove 1412 in the centre of mounting seat, to installation site sensor；Sidepiece baffle plate is with
Slide recess 1413 is provided between bottom surface.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and the embodiment is simultaneously not used to
The scope of the claims of the present invention is limited, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (6)
1. the DNA sequenators that a kind of CCD cameras single dimension is adjusted, it is characterised in which includes reaction warehouse component, with reaction
Storehouse component is arranged on the CCD phases that same level direction energy relative response storehouse component are moved forward and backward, taken pictures to reaction warehouse
Machine, drives the motion platform component of described CCD cameras linear reciprocation movement, and putting down of be connected with motion platform component
Platform support component；
Described motion platform component includes the lead screw guide rails component that motor is connected with motor output shaft, supports lead screw assembly
Support base, detection components；Be connected with nut on leading screw, nut is arranged on nut mounting seat, nut mounting seat installed in
On the slide block of guide rail contact, and camera movement is driven on slide block；
Described reaction warehouse component is arranged on the door frame on front side of platform component, and being provided with the top in the outside of door frame can
Upset reaction warehouse component, is connected with turnable basement in the lower end that may be reversed reaction warehouse component, and turnable basement is fixed on door frame
Middle part, may be reversed reaction warehouse component can rotate around described turnable basement, to take out or be put into reaction chip；
Described platform component includes vibration damper plate and is arranged on the first damping wing plate and second damping of vibration damper plate both sides
Wing plate, is respectively arranged with dampening assembly in the lower end of two damping wing plates；After installation is complete, platform component is in recess
Shape, reaction warehouse component are arranged on vibration damper plate, and two ends are contacted with two vibration damper plates respectively；
Described damping wing plate includes gripper shoe, and which has certain height, supports described reaction warehouse component；Gripper shoe one
The middle setting of the upper head plate of molding has the mounting groove of motion control unit, be mounted therein with the circuit board of motion control unit with
And various circuits；
It is reinforcing plate in the lower end of mounting groove, to open up mounting groove.
2. the DNA sequencer that CCD camera single dimension according to claim 1 is adjusted, it is characterised in that the output of motor
Axle is connected by shaft coupling with leading screw, and motor and leading screw junction are provided with support base, the end of leading screw be provided with bearing with
And axial positioning sleeve；
The upper end of described nut mounting seat is connected with connecting plate, and the upper end of connecting plate is connected with camera mounting seat；At bearing end
End arranges bearing friction top.
3. the DNA sequencer of the CCD camera single dimension adjustment stated according to claim 2, it is characterised in that described nut peace
The upper end of dress seat is provided with connecting plate installing hole, and mid portion is provided with through hole, to through leading screw；The surrounding of through hole is fastening
Screwed hole, to install with nut.
4. the DNA sequencer of the CCD camera single dimension adjustment stated according to claim 2, it is characterised in that described support base
Including leading screw installation sleeve, positioning step is set in which；The end of installation sleeve is connected with installing plate, to connect with motor end face
Connect.
5. the DNA sequencer of the CCD camera single dimension adjustment stated according to claim 2, it is characterised in that described detection group
Part includes detection plate fixed seat, and which is arranged on the sidepiece of connecting plate, and the end of detection plate fixed seat is connected with position detection plate；
The sidepiece of base installs position sensor mounting seat, and in position, the upper end of sensor installation seat arranges contiguous block, on contiguous block
The fixed position sensor in end.
6. the DNA sequencer of the CCD camera single dimension adjustment stated according to claim 5, it is characterised in that described position passes
Sensor mounting seat includes the screwed hole for being arranged on side, is mounting groove in the centre of mounting seat, to installation site sensor；
Slide recess is provided between sidepiece baffle plate and bottom surface.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716484.4A CN105368707B (en) | 2015-10-29 | 2015-10-29 | DNA sequencer with CCD camera adjusted in single-dimensional mode |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716484.4A CN105368707B (en) | 2015-10-29 | 2015-10-29 | DNA sequencer with CCD camera adjusted in single-dimensional mode |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105368707A CN105368707A (en) | 2016-03-02 |
| CN105368707B true CN105368707B (en) | 2017-04-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510716484.4A Expired - Fee Related CN105368707B (en) | 2015-10-29 | 2015-10-29 | DNA sequencer with CCD camera adjusted in single-dimensional mode |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114806798B (en) * | 2021-01-29 | 2025-04-29 | 深圳华大智造科技股份有限公司 | Facade motion structure and optical equipment |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703302A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102703314A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703311A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102766574A (en) * | 2012-05-24 | 2012-11-07 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
-
2015
- 2015-10-29 CN CN201510716484.4A patent/CN105368707B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703302A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102703314A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703312A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703311A (en) * | 2012-05-24 | 2012-10-03 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102766574A (en) * | 2012-05-24 | 2012-11-07 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
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| Publication number | Publication date |
|---|---|
| CN105368707A (en) | 2016-03-02 |
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Granted publication date: 20170412 Termination date: 20191029 |