CN105327384A - Absorbable medical biological membrane dressing and preparation method thereof - Google Patents
Absorbable medical biological membrane dressing and preparation method thereof Download PDFInfo
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- CN105327384A CN105327384A CN201510808374.0A CN201510808374A CN105327384A CN 105327384 A CN105327384 A CN 105327384A CN 201510808374 A CN201510808374 A CN 201510808374A CN 105327384 A CN105327384 A CN 105327384A
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Abstract
The invention discloses an absorbable medical biological membrane dressing. The dressing is mainly prepared from raw materials in parts by mass as follows: 50-70 parts of chitosan, 15-20 parts of gelatin, 5-9 parts of citronella, 3-7 parts of saffron, 4-8 parts of stephania sinica diels, 6-9 parts of red peony roots, 5-10 parts of vietnamese sophora roots, 3-6 parts of pipewort flowers, 5-8 parts of Henry Clematis roots, 6-11 parts of nerved elecampane roots, 4-7 parts of leaves and twigs of naked-flower beauty-berry and 3-8 parts of Trillium tschnoskii Maxim. The absorbable medical biological membrane dressing has good water absorption property and breathability, avoids wound adhesion, can slowly release traditional Chinese medicine active ingredients, prevents wound infection, promotes wound healing, has good biocompatibility and good mechanical properties in the wet environment and is biodegradable.
Description
Technical field
The present invention relates to field of medical products, particularly relate to a kind of Inhalant medical biological film dressing and preparation method thereof.
Background technology
Biological dressing can be divided into artificial creature's dressing and the large class of allosome tissue's wound-surface cover two.Common artificial creature's dressing is prepared by the material such as collagen, chitosan, hyaluronic acid, and it has hemostasis blood coagulation enhancing effect, can induce various kinds of cell proliferation and differentiation, and shortcoming is less stable and absorbs sepage indifferent etc.Dressing be can play temporary protection wound, the medical material of healing of protecting from infection, impel.Dressing can be divided into traditional dressing as defat cotton yarn, biological dressing and synthetic dressing etc. according to material.Compared with traditional dressing, biological dressing has reduction and infects, improves wound healing quality, alleviate the misery of patient and facilitate the advantages such as medical personnel operate.
Publication No. CN103027788A(application number 201310003806.1) Chinese patent literature disclose a kind of absorbability medical dressing and preparation method thereof, comprise the polymer matrix layer that there is elastic backing and be arranged on backing, described backing is by polyurethane, hyaluronic acid, hyaluronate, at least one composition in absorbability aliphatic polyester or poly butyric, polymer matrix layer is by polyacrylic acid, alginic acid, chitosan, water soluble polyamide, chitosan derivatives, at least one composition in polylysine or cellulose derivative, polymer matrix layer is provided with medicine layer, described medicine layer is by collagen protein, Fibrinogen, at least one composition in somatomedin or antibiotic.Publication No. CN102008740A(application number 201010573016.3) Chinese patent literature disclose a kind of Absorbable rod somatomedin combine dressing, this combine dressing is primarily of modified hydroxyethylcellulosadsorbing and the composite compound cellulose made of sodium alginate and somatomedin composition.
Chitosan and gelatin all have good biocompatibility and readily degradable, and both combinations are made into medical dressing, can meet the needs of healthcare givers and patient, safety non-toxic, degradable.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide and a kind ofly prepare simple, taking convenience, be easy to carry, Inhalant medical biological film dressing and preparation method thereof of easy clothes for a long time.
Technical scheme of the present invention is:
Inhalant medical biological film dressing, is prepared from primarily of following raw materials according, is mass parts: chitosan 50-70, gelatin 15-20, Herba Cymbopogonis Citrari 5-9, Stigma Croci 3-7, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 4-8, Radix Paeoniae Rubra 6-9, Radix Sophorae Tonkinensis 5-10, Flos Eriocauli 3-6, Radix Clematidis Henryi 5-8, Radix Inulae Nervosae 6-11, Folium et Ramulus Callicarpae nudiflorae 4-7, Rhizoma Trillii Tschonoskii 3-8.
In preferred scheme, chitosan 55-65, gelatin 16-19, Herba Cymbopogonis Citrari 6-8, Stigma Croci 4-6, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 5-7, Radix Paeoniae Rubra 7-8, Radix Sophorae Tonkinensis 6-9, Flos Eriocauli 4-5, Radix Clematidis Henryi 6-7, Radix Inulae Nervosae 7-10, Folium et Ramulus Callicarpae nudiflorae 5-6, Rhizoma Trillii Tschonoskii 4-7.
Be more preferably, chitosan 60, gelatin 17.5, Herba Cymbopogonis Citrari 7, Stigma Croci 5, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 6, Radix Paeoniae Rubra 7.5, Radix Sophorae Tonkinensis 7.5, Flos Eriocauli 4.5, Radix Clematidis Henryi 6.5, Radix Inulae Nervosae 8.5, Folium et Ramulus Callicarpae nudiflorae 5.5, Rhizoma Trillii Tschonoskii 5.5.
The preparation method of described Inhalant medical biological film dressing, step is:
(1) get Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), Radix Paeoniae Rubra and Radix Sophorae Tonkinensis to clean, mix thoroughly with Rhizoma Zingiberis Recens juice, with face block parcel medical material, be placed in hot charcoal fire, take out when simmering black to yellowish complexion, the face block on removing surface, dries in the air cool, section;
(2) Radix Clematidis Henryi and Radix Inulae Nervosae are cleaned, infiltrate, section, mixes thoroughly with rice vinegar together with the Herba Cymbopogonis Citrari of cutting, leaves standstill 30-50 minute (preferred, to leave standstill 40 minutes), then adds rice-washed water heated and boiled, take out, dry;
(3) get Rhizoma Trillii Tschonoskii clean run through, section, Oleum sesami is placed in oil cauldron, boil micro-boiling, add Rhizoma Trillii Tschonoskii sheet, stir-fry to surface and turn yellow, taking-up is dried in the air cool, pulverizes and sieves together with step (1) (2) made raw material, dries, add 80% ethanol to be heated to 100 DEG C reflux, extract, 4-6 hour (preferred, reflux, extract, 5 hours), sucking filtration, collect filtrate, filtering residue is for subsequent use, and filtrate is concentrated into without alcohol taste, obtained extract;
(4) Stigma Croci, Flos Eriocauli are pulverized together with Folium et Ramulus Callicarpae nudiflorae and make medicated powder, add decocting in water twice together with step (3) made filtering residue, boiling amount of water for twice, to be the 6-8 of total raw material quality doubly (preferred, boil 7 times that amount of water is total raw material quality for twice), each big fire turns little fire after boiling and boils 30 minutes, filters, collects filtrate, merge twice and filter gained filtrate, be condensed into clear paste, extract stirring and evenly mixing made with step (3), sucking filtration, dry, pulverize and make medicated powder;
(5) get chitosan 5% acetic acid aqueous solution to dissolve, after filtering deaeration, obtained mass concentration is the chitosan solution of 3%, get the deionized water dissolving of gelatin 40-60 DEG C, filter, obtained mass concentration is the gelatin solution of 4%, made for step (4) medicated powder deionized water dissolving is mixed with the medicinal liquid that concentration is 2.0g/mL, filters, then the glutaraldehyde solution deionized water of 50% dilution is mixed with the glutaraldehyde solution that mass concentration is 1.5%;
(6) by step (5) made chitosan solution and gelatin solution mixing, stir, add glycerol to stir evenly, add step (5) made medicinal liquid again, finally add the glutaraldehyde solution of 1.5%, abundant stirring 20-30 minute, rotating speed 800-1200 rev/min (preferred, rotating speed 900-1100 rev/min; Be more preferably, rotating speed 1000 revs/min), evacuation and centrifugal degassing, pours in mould, dry at 50 DEG C, and take off film, soak 1-2 minute with 5% sodium hydrate aqueous solution, then be washed to neutrality, air-dry, sterilizing, obtains biomembrane dressing.
The preparation method of described Inhalant medical biological film dressing, step (2) heated and boiled 12-18 minute (preferably, heated and boiled 14-16 minute; Be more preferably, heated and boiled 15 minutes).
The preparation method of described Inhalant medical biological film dressing, step (3) was pulverized 140-220 mesh sieve and (preferably, was pulverized 160-200 mesh sieve; Be more preferably, pulverized 180 mesh sieves).
The preparation method of described Inhalant medical biological film dressing, the 5-7 that the amount of step (3) 80% ethanol is institute's lixiviate raw materials quality is (preferred, 6 times that the amount of 80% ethanol is institute's lixiviate raw materials quality) doubly.
The preparation method of described Inhalant medical biological film dressing, step (4) was pulverized 400-800 mesh sieve and (preferably, was pulverized 500-600 mesh sieve; Be more preferably, pulverized 700 mesh sieves).
The preparation method of described Inhalant medical biological film dressing, step (6) fully stirs 23-27 minute (preferred, fully to stir 25 minutes).
The preparation method of described Inhalant medical biological film dressing, volume ratio when each solution of step (6) mixes is chitosan: gelatin: medicinal liquid: glutaraldehyde=1:3:1:0.03.
Inhalant medical biological film dressing preparation method science provided by the invention environmental protection, cost is low.In addition, excellent results of the present invention also shows:
1, the present invention has good water absorption and breathability, adhesion wound;
2, the present invention can slow releasing active ingredient of Chinese herbs wherein, prevents wound infection, promotes wound healing;
3, good biocompatibility of the present invention, wet environment good mechanical property, and biodegradable.
Detailed description of the invention
Describe technical scheme of the present invention in detail below in conjunction with embodiment and experimental example, but protection domain is not limited thereto.
embodiment 1inhalant medical biological film dressing, is prepared from (every part of 10g) primarily of following raw materials according: chitosan 50 parts, 15 parts, gelatin, 5 parts, Herba Cymbopogonis Citrari, Stigma Croci 3 parts, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 4 parts, Radix Paeoniae Rubra 6 parts, Radix Sophorae Tonkinensis 5 parts, Flos Eriocauli 3 parts, Radix Clematidis Henryi 5 parts, Radix Inulae Nervosae 6 parts, Folium et Ramulus Callicarpae nudiflorae 4 parts, Rhizoma Trillii Tschonoskii 3 parts.
The preparation method of above-mentioned Inhalant medical biological film dressing is:
(1) get Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), Radix Paeoniae Rubra and Radix Sophorae Tonkinensis to clean, mix thoroughly with Rhizoma Zingiberis Recens juice, with face block parcel medical material, be placed in hot charcoal fire, take out when simmering black to yellowish complexion, the face block on removing surface, dries in the air cool, section;
(2) Radix Clematidis Henryi and Radix Inulae Nervosae are cleaned, infiltrate, section, mixes thoroughly with rice vinegar together with the Herba Cymbopogonis Citrari of cutting, leaves standstill 40 minutes, then adds rice-washed water heated and boiled 12 minutes, takes out, and dries;
(3) get Rhizoma Trillii Tschonoskii clean run through, section, Oleum sesami is placed in oil cauldron, boil micro-boiling, add Rhizoma Trillii Tschonoskii sheet, stir-fry to surface and turn yellow, taking-up is dried in the air cool, pulverizes 140 mesh sieves together with step (1) (2) made raw material, dries, add 80% ethanol and be heated to 100 DEG C of reflux, extract, 5 hours (5 times that the amount of 80% ethanol is institute's lixiviate raw materials quality), sucking filtration, collect filtrate, filtering residue is for subsequent use, filtrate is concentrated into without alcohol taste, obtained extract;
(4) Stigma Croci, Flos Eriocauli are pulverized together with Folium et Ramulus Callicarpae nudiflorae make medicated powder, add decocting in water twice together with step (3) made filtering residue, boil 7 times that amount of water is total raw material quality for twice, each big fire turns little fire after boiling and boils 30 minutes, filter, collect filtrate, merge twice and filter gained filtrate, be condensed into clear paste, extract stirring and evenly mixing made with step (3), sucking filtration, dry, pulverize 400 mesh sieves and made medicated powder;
(5) get chitosan 5% acetic acid aqueous solution to dissolve, after filtering deaeration, obtained mass concentration is the chitosan solution of 3%, get the deionized water dissolving of gelatin 40-60 DEG C, filter, obtained mass concentration is the gelatin solution of 4%, made for step (4) medicated powder deionized water dissolving is mixed with the medicinal liquid that concentration is 2.0g/mL, filters, then the glutaraldehyde solution deionized water of 50% dilution is mixed with the glutaraldehyde solution that mass concentration is 1.5%;
(6) by step (5) made chitosan solution and gelatin solution mixing, stir, add glycerol to stir evenly, add step (5) made medicinal liquid again, finally add the glutaraldehyde solution (volume ratio of each solution is chitosan: gelatin: medicinal liquid: glutaraldehyde=1:3:1:0.03) of 1.5%, abundant stirring 20 minutes, rotating speed 1000 revs/min, evacuation and centrifugal degassing, pour in mould, dry at 50 DEG C, take off film, soak 1-2 minute with 5% sodium hydrate aqueous solution, be washed to neutrality again, air-dry, sterilizing, obtains biomembrane dressing.
embodiment 2inhalant medical biological film dressing, is prepared from (every part of 10g) primarily of following raw materials according: chitosan 70 parts, 20 parts, gelatin, 9 parts, Herba Cymbopogonis Citrari, Stigma Croci 7 parts, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 8 parts, Radix Paeoniae Rubra 9 parts, Radix Sophorae Tonkinensis 10 parts, Flos Eriocauli 6 parts, Radix Clematidis Henryi 8 parts, Radix Inulae Nervosae 11 parts, Folium et Ramulus Callicarpae nudiflorae 7 parts, Rhizoma Trillii Tschonoskii 8 parts.
The preparation method of Inhalant medical biological film dressing is:
(1) get Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), Radix Paeoniae Rubra and Radix Sophorae Tonkinensis to clean, mix thoroughly with Rhizoma Zingiberis Recens juice, with face block parcel medical material, be placed in hot charcoal fire, take out when simmering black to yellowish complexion, the face block on removing surface, dries in the air cool, section;
(2) Radix Clematidis Henryi and Radix Inulae Nervosae are cleaned, infiltrate, section, mixes thoroughly with rice vinegar together with the Herba Cymbopogonis Citrari of cutting, leaves standstill 40 minutes, then adds rice-washed water heated and boiled 18 minutes, takes out, and dries;
(3) get Rhizoma Trillii Tschonoskii clean run through, section, Oleum sesami is placed in oil cauldron, boil micro-boiling, add Rhizoma Trillii Tschonoskii sheet, stir-fry to surface and turn yellow, taking-up is dried in the air cool, pulverizes 220 mesh sieves together with step (1) (2) made raw material, dries, add 80% ethanol and be heated to 100 DEG C of reflux, extract, 5 hours (7 times that the amount of 80% ethanol is institute's lixiviate raw materials quality), sucking filtration, collect filtrate, filtering residue is for subsequent use, filtrate is concentrated into without alcohol taste, obtained extract;
(4) Stigma Croci, Flos Eriocauli are pulverized together with Folium et Ramulus Callicarpae nudiflorae make medicated powder, add decocting in water twice together with step (3) made filtering residue, boil 7 times that amount of water is total raw material quality for twice, each big fire turns little fire after boiling and boils 30 minutes, filter, collect filtrate, merge twice and filter gained filtrate, be condensed into clear paste, extract stirring and evenly mixing made with step (3), sucking filtration, dry, pulverize 800 mesh sieves and made medicated powder;
(5) get chitosan 5% acetic acid aqueous solution to dissolve, after filtering deaeration, obtained mass concentration is the chitosan solution of 3%, get the deionized water dissolving of gelatin 40-60 DEG C, filter, obtained mass concentration is the gelatin solution of 4%, made for step (4) medicated powder deionized water dissolving is mixed with the medicinal liquid that concentration is 2.0g/mL, filters, then the glutaraldehyde solution deionized water of 50% dilution is mixed with the glutaraldehyde solution that mass concentration is 1.5%;
(6) by step (5) made chitosan solution and gelatin solution mixing, stir, add glycerol to stir evenly, add step (5) made medicinal liquid again, finally add the glutaraldehyde solution (volume ratio of each solution is chitosan: gelatin: medicinal liquid: glutaraldehyde=1:3:1:0.03) of 1.5%, abundant stirring 30 minutes, rotating speed 1000 revs/min, evacuation and centrifugal degassing, pour in mould, dry at 50 DEG C, take off film, soak 1-2 minute with 5% sodium hydrate aqueous solution, be washed to neutrality again, air-dry, sterilizing, obtains biomembrane dressing.
Typical case: xxx, female, 4 years old.When playing, cervical region is burnt by firecrackers, and pain is obvious, constantly cries and screams, clinic checks superficial second degree burn, and cervical region local skin is red and swollen, hot, wound surface smears burn cream after carrying out clean, and uses the dressing of the present embodiment gained Inhalant medical biological film to spread on affected part to use and fix, after 2 days, pain obviously alleviates, and redness disappears, there is newborn epithelial tissue at wound surface place, after 10 days, wound healing, does not leave obvious cicatrix.
embodiment 3inhalant medical biological film dressing, is prepared from (every part of 10g) primarily of following raw materials according: chitosan 55 parts, 16 parts, gelatin, 6 parts, Herba Cymbopogonis Citrari, Stigma Croci 4 parts, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 5 parts, Radix Paeoniae Rubra 7 parts, Radix Sophorae Tonkinensis 6 parts, Flos Eriocauli 4 parts, Radix Clematidis Henryi 6 parts, Radix Inulae Nervosae 7 parts, Folium et Ramulus Callicarpae nudiflorae 5 parts, Rhizoma Trillii Tschonoskii 4 parts.
The preparation method of Inhalant medical biological film dressing is:
(1) get Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), Radix Paeoniae Rubra and Radix Sophorae Tonkinensis to clean, mix thoroughly with Rhizoma Zingiberis Recens juice, with face block parcel medical material, be placed in hot charcoal fire, take out when simmering black to yellowish complexion, the face block on removing surface, dries in the air cool, section;
(2) Radix Clematidis Henryi and Radix Inulae Nervosae are cleaned, infiltrate, section, mixes thoroughly with rice vinegar together with the Herba Cymbopogonis Citrari of cutting, leaves standstill 40 minutes, then adds rice-washed water heated and boiled 14 minutes, takes out, and dries;
(3) get Rhizoma Trillii Tschonoskii clean run through, section, Oleum sesami is placed in oil cauldron, boil micro-boiling, add Rhizoma Trillii Tschonoskii sheet, stir-fry to surface and turn yellow, taking-up is dried in the air cool, pulverizes 160 mesh sieves together with step (1) (2) made raw material, dries, add 80% ethanol and be heated to 100 DEG C of reflux, extract, 5 hours (5 times that the amount of 80% ethanol is institute's lixiviate raw materials quality), sucking filtration, collect filtrate, filtering residue is for subsequent use, filtrate is concentrated into without alcohol taste, obtained extract;
(4) Stigma Croci, Flos Eriocauli are pulverized together with Folium et Ramulus Callicarpae nudiflorae make medicated powder, add decocting in water twice together with step (3) made filtering residue, boil 7 times that amount of water is total raw material quality for twice, each big fire turns little fire after boiling and boils 30 minutes, filter, collect filtrate, merge twice and filter gained filtrate, be condensed into clear paste, extract stirring and evenly mixing made with step (3), sucking filtration, dry, pulverize 500 mesh sieves and made medicated powder;
(5) get chitosan 5% acetic acid aqueous solution to dissolve, after filtering deaeration, obtained mass concentration is the chitosan solution of 3%, get the deionized water dissolving of gelatin 40-60 DEG C, filter, obtained mass concentration is the gelatin solution of 4%, made for step (4) medicated powder deionized water dissolving is mixed with the medicinal liquid that concentration is 2.0g/mL, filters, then the glutaraldehyde solution deionized water of 50% dilution is mixed with the glutaraldehyde solution that mass concentration is 1.5%;
(6) by step (5) made chitosan solution and gelatin solution mixing, stir, add glycerol to stir evenly, add step (5) made medicinal liquid again, finally add the glutaraldehyde solution (volume ratio of each solution is chitosan: gelatin: medicinal liquid: glutaraldehyde=1:3:1:0.03) of 1.5%, abundant stirring 23 minutes, rotating speed 1000 revs/min, evacuation and centrifugal degassing, pour in mould, dry at 50 DEG C, take off film, soak 1-2 minute with 5% sodium hydrate aqueous solution, be washed to neutrality again, air-dry, sterilizing, obtains biomembrane dressing.
Typical case: xxx, man, 36 years old.On the road of cycle to work, accidentally drop from car, cause extremity large area to rub wound, cuticular breakdown is serious, after carrying out cleaning and disinfection, uses the dressing of the present embodiment gained Inhalant medical biological film to wound, a small amount of sepage and oozing of blood is had after 1 day, after 2 days, wound surface dry tack free, there is crust at wound surface edge, after 3 days, wound surface is all formed a scab, after 6 days, decrustation, wound surface heals completely.
embodiment 4inhalant medical biological film dressing, is prepared from (every part of 10g) primarily of following raw materials according: chitosan 65 parts, 19 parts, gelatin, 8 parts, Herba Cymbopogonis Citrari, Stigma Croci 6 parts, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 7 parts, Radix Paeoniae Rubra 8 parts, Radix Sophorae Tonkinensis 9 parts, Flos Eriocauli 5 parts, Radix Clematidis Henryi 7 parts, Radix Inulae Nervosae 10 parts, Folium et Ramulus Callicarpae nudiflorae 6 parts, Rhizoma Trillii Tschonoskii 7 parts.
The preparation method of Inhalant medical biological film dressing is:
(1) get Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), Radix Paeoniae Rubra and Radix Sophorae Tonkinensis to clean, mix thoroughly with Rhizoma Zingiberis Recens juice, with face block parcel medical material, be placed in hot charcoal fire, take out when simmering black to yellowish complexion, the face block on removing surface, dries in the air cool, section;
(2) Radix Clematidis Henryi and Radix Inulae Nervosae are cleaned, infiltrate, section, mixes thoroughly with rice vinegar together with the Herba Cymbopogonis Citrari of cutting, leaves standstill 40 minutes, then adds rice-washed water heated and boiled 16 minutes, takes out, and dries;
(3) get Rhizoma Trillii Tschonoskii clean run through, section, Oleum sesami is placed in oil cauldron, boil micro-boiling, add Rhizoma Trillii Tschonoskii sheet, stir-fry to surface and turn yellow, taking-up is dried in the air cool, pulverizes 200 mesh sieves together with step (1) (2) made raw material, dries, add 80% ethanol and be heated to 100 DEG C of reflux, extract, 5 hours (7 times that the amount of 80% ethanol is institute's lixiviate raw materials quality), sucking filtration, collect filtrate, filtering residue is for subsequent use, filtrate is concentrated into without alcohol taste, obtained extract;
(4) Stigma Croci, Flos Eriocauli are pulverized together with Folium et Ramulus Callicarpae nudiflorae make medicated powder, add decocting in water twice together with step (3) made filtering residue, boil 7 times that amount of water is total raw material quality for twice, each big fire turns little fire after boiling and boils 30 minutes, filter, collect filtrate, merge twice and filter gained filtrate, be condensed into clear paste, extract stirring and evenly mixing made with step (3), sucking filtration, dry, pulverize 600 mesh sieves and made medicated powder;
(5) get chitosan 5% acetic acid aqueous solution to dissolve, after filtering deaeration, obtained mass concentration is the chitosan solution of 3%, get the deionized water dissolving of gelatin 40-60 DEG C, filter, obtained mass concentration is the gelatin solution of 4%, made for step (4) medicated powder deionized water dissolving is mixed with the medicinal liquid that concentration is 2.0g/mL, filters, then the glutaraldehyde solution deionized water of 50% dilution is mixed with the glutaraldehyde solution that mass concentration is 1.5%;
(6) by step (5) made chitosan solution and gelatin solution mixing, stir, add glycerol to stir evenly, add step (5) made medicinal liquid again, finally add the glutaraldehyde solution (volume ratio of each solution is chitosan: gelatin: medicinal liquid: glutaraldehyde=1:3:1:0.03) of 1.5%, abundant stirring 27 minutes, rotating speed 1000 revs/min, evacuation and centrifugal degassing, pour in mould, dry at 50 DEG C, take off film, soak 1-2 minute with 5% sodium hydrate aqueous solution, be washed to neutrality again, air-dry, sterilizing, obtains biomembrane dressing.
embodiment 5inhalant medical biological film dressing, is prepared from (every part of 10g) primarily of following raw materials according: chitosan 60 parts, 17.5 parts, gelatin, 7 parts, Herba Cymbopogonis Citrari, Stigma Croci 5 parts, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 6 parts, Radix Paeoniae Rubra 7.5 parts, Radix Sophorae Tonkinensis 7.5 parts, Flos Eriocauli 4.5 parts, Radix Clematidis Henryi 6.5 parts, Radix Inulae Nervosae 8.5 parts, Folium et Ramulus Callicarpae nudiflorae 5.5 parts, Rhizoma Trillii Tschonoskii 5.5 parts.
The preparation method of Inhalant medical biological film dressing is:
(1) get Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), Radix Paeoniae Rubra and Radix Sophorae Tonkinensis to clean, mix thoroughly with Rhizoma Zingiberis Recens juice, with face block parcel medical material, be placed in hot charcoal fire, take out when simmering black to yellowish complexion, the face block on removing surface, dries in the air cool, section;
(2) Radix Clematidis Henryi and Radix Inulae Nervosae are cleaned, infiltrate, section, mixes thoroughly with rice vinegar together with the Herba Cymbopogonis Citrari of cutting, leaves standstill 40 minutes, then adds rice-washed water heated and boiled 15 minutes, takes out, and dries;
(3) get Rhizoma Trillii Tschonoskii clean run through, section, Oleum sesami is placed in oil cauldron, boil micro-boiling, add Rhizoma Trillii Tschonoskii sheet, stir-fry to surface and turn yellow, taking-up is dried in the air cool, pulverizes 180 mesh sieves together with step (1) (2) made raw material, dries, add 80% ethanol and be heated to 100 DEG C of reflux, extract, 5 hours (6 times that the amount of 80% ethanol is institute's lixiviate raw materials quality), sucking filtration, collect filtrate, filtering residue is for subsequent use, filtrate is concentrated into without alcohol taste, obtained extract;
(4) Stigma Croci, Flos Eriocauli are pulverized together with Folium et Ramulus Callicarpae nudiflorae make medicated powder, add decocting in water twice together with step (3) made filtering residue, boil 7 times that amount of water is total raw material quality for twice, each big fire turns little fire after boiling and boils 30 minutes, filter, collect filtrate, merge twice and filter gained filtrate, be condensed into clear paste, extract stirring and evenly mixing made with step (3), sucking filtration, dry, pulverize 700 mesh sieves and made medicated powder;
(5) get chitosan 5% acetic acid aqueous solution to dissolve, after filtering deaeration, obtained mass concentration is the chitosan solution of 3%, get the deionized water dissolving of gelatin 40-60 DEG C, filter, obtained mass concentration is the gelatin solution of 4%, made for step (4) medicated powder deionized water dissolving is mixed with the medicinal liquid that concentration is 2.0g/mL, filters, then the glutaraldehyde solution deionized water of 50% dilution is mixed with the glutaraldehyde solution that mass concentration is 1.5%;
(6) by step (5) made chitosan solution and gelatin solution mixing, stir, add glycerol to stir evenly, add step (5) made medicinal liquid again, finally add the glutaraldehyde solution (volume ratio of each solution is chitosan: gelatin: medicinal liquid: glutaraldehyde=1:3:1:0.03) of 1.5%, abundant stirring 25 minutes, rotating speed 1000 revs/min, evacuation and centrifugal degassing, pour in mould, dry at 50 DEG C, take off film, soak 1-2 minute with 5% sodium hydrate aqueous solution, be washed to neutrality again, air-dry, sterilizing, obtains biomembrane dressing.
Pharmacology and the application of the present invention's primary raw material used are as follows:
Chitosan: also known as chitosan, a kind of white or the translucent lamellar of canescence or powdery solid.Medical application mainly contains and promotes blood coagulation and wound healing, as the sustained-release matrix of medicine, for artificial tissue and organ, has immunoregulatory activity.The premium properties such as the biological functionality of this natural polymer and the compatibility, blood compatibility, safety, microbic resolvability all have application at numerous areas such as medicine, food, chemical industry, cosmetics, water treatment, METAL EXTRACTION and recovery, biochemistry and biomedical engineerings.
Gelatin: do not have fixing structure and relative molecular weight, is degraded by the collagenous portion in the connective tissues such as animal skin, bone, sarolemma, flesh evil spirit and becomes thin slice or the powder of white or faint yellow, translucent, micro-band gloss.There is gelation, retentiveness, film property, emulsibility and foaming characteristic etc.
Herba Cymbopogonis Citrari: base is former: be the herb of grass Herba Cymbopogonis Citrari.Nature and flavor: pungent, temperature.Function cures mainly: expelling wind and removing dampness, reducing swelling and alleviating pain.For rheumatalgia, headache, stomachache, stomachache, diarrhoea, menoxenia, puerperal edema, traumatic injury congestion swells and ache.
Stigma Croci: base is former: be the dry stigma of Iridaceae crocus plant Stigma Croci.Nature and flavor: sweet are flat.Gui Jing: GUIXIN, Liver Channel.Function cures mainly: blood circulation promoting and blood stasis dispelling, removing pathogenic heat from blood and toxic substance from the body, resolving stagnation for tranquilization.For amenorrhea lump in the abdomen, postpartum stagnation, maculae caused by violent heat pathogen, melancholy painful abdominal mass is vexed, and palpitation with fear is gone mad.
Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae): base is former: be the tuber of menispermaceous plants Radix Stephaniae Japonicae.Nature and flavor: bitter, cold.Function cures mainly: heat-clearing and toxic substances removing, benefiting stomach and stopping pain, dissipating blood stasis for subsidence of swelling.Control exogenous cough, pharyngalgia, aphtha of the mouth and tongue, vomiting and diarrhoea, dysentery, stomachache, swollen ulcer drug, traumatic injury.
Radix Paeoniae Rubra: base is former: be the root of ranunculaceae plant Radix Paeoniae or river Radix Paeoniae Rubra.Nature and flavor: bitter, are slightly cold.Gui Jing: return Liver Channel.Function cures mainly: clearing away heat and cooling blood, eliminating stasis to stop pain.For maculae caused by violent heat pathogen, hematemesis and epistaxis, conjunctival congestion and swelling pain, hypochondriac pain due to stagnation of liverQI, amenorrhea dysmenorrhea, lump in the abdomen is suffered from abdominal pain, injury from falling down, carbuncle skin infection.
Radix Sophorae Tonkinensis: base is former: the root being mainly leguminous plant root of subprostrate sophora.Nature and flavor: bitter, cold, poisonous.Gui Jing: attach to the lung and stomach meridians.Function cures mainly: heat-clearing and toxic substances removing, detumescence sore-throat relieving.Pent up for fire-toxin, laryngopharynx swelling and pain, gingivitis.
Flos Eriocauli: base is former: be the inflorescence of the band scape of Eriocaulaceae plant Flos Eriocauli.Nature and flavor: pungent, sweet are flat.Gui Jing: return liver, lung meridian.Function cures mainly: dispelling wind and heat pathogens, improving eyesight, nebula removing.For wind heat conjunctival congestion, swell and ache photophobia, nebula of looking unfamiliar film, headache due to pathogenic wind-heat.
Radix Clematidis Henryi: base is former: be root or the leaf of cohosh henry clematis.Nature and flavor: arduous, temperature, nontoxic.Function cures mainly: promoting flow of QI and blood, anti-inflammation, and the pain such as enemy, stomach, abdomen, muscle and joint all have analgesic effect.Also tracheitis is controlled, furuncle swelling toxin, traumatic injury.
Radix Inulae Nervosae: base is former: be the root of feverfew Inula Narvosa.Nature and flavor: acrid in the mouth is bitter, warm in nature.Function cures mainly: cold-dispelling, and removing food stagnancy is stagnant, the meridian dredging.Control coldness and pain in the epigastrium, dyspeptic abdominal distention, dysphagic, stomachache, the empty hyperhidrosis of body, cold cough, rheumatism beriberi.
Folium et Ramulus Callicarpae nudiflorae: base is former: be the leaf with twig of Verbenaceae Callicarpa nudiflora.Nature and flavor: puckery micro-arduous, flat.Gui Jing: heart channel.Function cures mainly: dissipating blood stasis stops blooding, removing toxic substances and promoting subsidence of swelling.Main epistaxis, hemoptysis, spit blood, have blood in stool, traumatic injury congestive edema, traumatic hemorrhage, burn due to hot liquid or fire, sore festers.
Rhizoma Trillii Tschonoskii: base is former: be the rhizome of liliaceous plant Trillium tschonoskii Maxim and Jilin Trillium tschonoskii Maxim.Nature and flavor: sweet, temperature.Slightly poisonous.Function cures mainly: sedation-analgesia, hemostasis, removing toxic substances.For dizziness and headache, hypertension, neurasthenia, traumatic injury, lumbago and skelalgia, menoxenia, metrorrhagia, furuncle is controlled in external.
test example 1the physicochemical property of gained Inhalant medical biological film of the present invention dressing (embodiment 5) and the mensuration of biocompatibility:
1, the mensuration of physicochemical property
Thickness: select the made biomembrane dressing of the present invention 4, on three films, its thickness is measured in Stochastic choice 5 sites, calculates thickness geometric mean.
Water vapor permeance: add triumph saline in penetrating cup, with the made biomembrane dressing sealing of the present invention, weigh (w
1), in 37 DEG C of calorstats, be incubated 24h, then weigh (w
2), calculate the penetrating amount of steam, the meansigma methods after calculating is got in test repetition for 5 times.
Steam penetrating amount=(w
1-w
2)/(A × d) wherein A be the effective penetrating area of film, d is the diameter of penetrating cup.
Water absorption rate: weigh made for the present invention biomembrane dressing w
1, then the phosphate buffer (pH=7.4) being placed in 37 DEG C soaks 24h, suck the water on film with filter paper, weigh w
2, calculate water absorption rate, the meansigma methods after calculating is got in test repetition for 5 times.Water absorption rate=((w
2-w
1)/w
1) × 100%
2, the mensuration of biocompatibility
Subjects: Wistar rat 120, body weight is about about 250g, male and female half and half.
Test method: (1) histocompatibility gets Wistar rat 40, shave off the hair of rat right hind leg, sterilization depilation district, lumbar injection 3% pentobarbital sodium is anaesthetized, scalpel scratches thigh skin, expose vastus meat, vastus meat marks one otch, made for the present invention biomembrane dressing is cut into the square block of 5 × 5mm, rat back leg stock intramuscular is implanted after normal saline soaks, sew up wound, respectively at postoperative 7 days, 14 days, 21 days, 30 days, within 40 days, respectively put to death 8, carry out histological observation.
(2) wound healing effect gets Wistar rat 20, shave off the hair of rat back, sterilization depilation district, lumbar injection 3% pentobarbital sodium is anaesthetized, at difference 1cm place, interval, spinal column both sides, symmetry deducts the full thick-layer skin that diameter is 2cm, side is that experimental group face uses the made biomembrane dressing of the present invention, side is that matched group face uses sterile vaseline gauze, wrap up with gauze, within every 3 days, observe and record a wound surface area, to hinder wound surface area that rear first day records for initial area, calculating Wound healing rate.Wound healing rate=(initial area-revolution mark)/initial area × 100%
(3) hemostatic function gets Wistar rat 20, is divided into two groups at random, experimental group and matched group, shave off the hair of rat hindlimb, sterilization depilation district, lumbar injection 3% pentobarbital sodium is anaesthetized, aseptically cut off hind leg cortex and be about 1cm, expose rat femoral, manufacture hemorrhage wound surface with knife blade crosscut femoral artery, the made biomembrane dressing of experimental group the present invention applies pressure hemostasis, matched group antiseptic gauze stops blooding, record bleeding stopping period, in triplicate, is averaged bleeding stopping period.
Result of the test: 1. test records the thickness of the made biomembrane dressing of the present invention is 120 μm, and steam Penetration ration is 2014(gm
-2d
-1), water absorption rate is 354%.
The rat of 2. implanting the made biomembrane dressing of the present invention all survives, and wound surface is without infection, and local skin is without congestion and edema, within postoperative 7 days, enclosed mass can be touched, within postoperative 14 days, thigh enclosed mass obviously reduces, and within postoperative 21 days, enclosed mass disappears, and rat ingests, spirit, activity are all normal.Illustrate that products made thereby of the present invention is to Wistar rat avirulence.Postoperative 40 days, have a few fibres tissue and multinucleated giant cell in stock muscle incision, have the milky material engulfed in cell cytosol, the biomembrane dressing of implantation is absorbed.
Wound healing effectiveness results is as shown in table 1, experimental group face wound healing comparatively matched group face is fast, and postoperative 3 days, experimental group wound surface obviously shrank, clean without sepage, and matched group still has a small amount of sepage to ooze out, postoperative 12 days, remove the dressing on wound surface surface with normal saline, visible experimental group wound surface skin heals substantially, wound surface is dry, and matched group wound surface still has part not heal, and illustrates that the made biomembrane dressing of the present invention has wound healing effect.
The comparison (%) of table 1 different time two groups of Wound healing rates
The display of hemostasis test result, the experimental group hemostasis time used is 73 seconds, and the matched group hemostasis time used is 126 seconds, and result illustrates the made raw film dressing haemostatic effect of the present invention, and comparatively antiseptic gauze is good.Itself and wound surface are fitted better, and energy effective sealing wound surface is hemorrhage.
It should be pointed out that detailed description of the invention is the more representational example of the present invention, obvious technical scheme of the present invention is not limited to above-described embodiment, can also have a lot of distortion.Those of ordinary skill in the art, with the present invention clear and definite disclosed or according to undoubted the obtaining of the written description of file, the claimed scope of this patent all should be thought.
Claims (10)
1. Inhalant medical biological film dressing, is characterized in that, is prepared from primarily of following raw materials according, be mass parts: chitosan 50-70, gelatin 15-20, Herba Cymbopogonis Citrari 5-9, Stigma Croci 3-7, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 4-8, Radix Paeoniae Rubra 6-9, Radix Sophorae Tonkinensis 5-10, Flos Eriocauli 3-6, Radix Clematidis Henryi 5-8, Radix Inulae Nervosae 6-11, Folium et Ramulus Callicarpae nudiflorae 4-7, Rhizoma Trillii Tschonoskii 3-8.
2. Inhalant medical biological film dressing as claimed in claim 1, is characterized in that, chitosan 55-65, gelatin 16-19, Herba Cymbopogonis Citrari 6-8, Stigma Croci 4-6, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 5-7, Radix Paeoniae Rubra 7-8, Radix Sophorae Tonkinensis 6-9, Flos Eriocauli 4-5, Radix Clematidis Henryi 6-7, Radix Inulae Nervosae 7-10, Folium et Ramulus Callicarpae nudiflorae 5-6, Rhizoma Trillii Tschonoskii 4-7.
3. Inhalant medical biological film dressing as claimed in claim 1, is characterized in that, chitosan 60, gelatin 17.5, Herba Cymbopogonis Citrari 7, Stigma Croci 5, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) 6, Radix Paeoniae Rubra 7.5, Radix Sophorae Tonkinensis 7.5, Flos Eriocauli 4.5, Radix Clematidis Henryi 6.5, Radix Inulae Nervosae 8.5, Folium et Ramulus Callicarpae nudiflorae 5.5, Rhizoma Trillii Tschonoskii 5.5.
4. the preparation method of the Inhalant medical biological film dressing as described in as arbitrary in claim 1-3, it is characterized in that, step is:
(1) get Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae), Radix Paeoniae Rubra and Radix Sophorae Tonkinensis to clean, mix thoroughly with Rhizoma Zingiberis Recens juice, with face block parcel medical material, be placed in hot charcoal fire, take out when simmering black to yellowish complexion, the face block on removing surface, dries in the air cool, section;
(2) Radix Clematidis Henryi and Radix Inulae Nervosae are cleaned, infiltrate, section, mixes thoroughly with rice vinegar together with the Herba Cymbopogonis Citrari of cutting, leaves standstill 30-50 minute (preferred, to leave standstill 40 minutes), then adds rice-washed water heated and boiled, take out, dry;
(3) get Rhizoma Trillii Tschonoskii clean run through, section, Oleum sesami is placed in oil cauldron, boil micro-boiling, add Rhizoma Trillii Tschonoskii sheet, stir-fry to surface and turn yellow, taking-up is dried in the air cool, pulverizes and sieves together with step (1) (2) made raw material, dries, add 80% ethanol to be heated to 100 DEG C reflux, extract, 4-6 hour (preferred, reflux, extract, 5 hours), sucking filtration, collect filtrate, filtering residue is for subsequent use, and filtrate is concentrated into without alcohol taste, obtained extract;
(4) Stigma Croci, Flos Eriocauli are pulverized together with Folium et Ramulus Callicarpae nudiflorae and make medicated powder, add decocting in water twice together with step (3) made filtering residue, boiling amount of water for twice, to be the 6-8 of total raw material quality doubly (preferred, boil 7 times that amount of water is total raw material quality for twice), each big fire turns little fire after boiling and boils 30 minutes, filters, collects filtrate, merge twice and filter gained filtrate, be condensed into clear paste, extract stirring and evenly mixing made with step (3), sucking filtration, dry, pulverize and make medicated powder;
(5) get chitosan 5% acetic acid aqueous solution to dissolve, after filtering deaeration, obtained mass concentration is the chitosan solution of 3%, get the deionized water dissolving of gelatin 40-60 DEG C, filter, obtained mass concentration is the gelatin solution of 4%, made for step (4) medicated powder deionized water dissolving is mixed with the medicinal liquid that concentration is 2.0g/mL, filters, then the glutaraldehyde solution deionized water of 50% dilution is mixed with the glutaraldehyde solution that mass concentration is 1.5%;
(6) by step (5) made chitosan solution and gelatin solution mixing, stir, add glycerol to stir evenly, add step (5) made medicinal liquid again, finally add the glutaraldehyde solution of 1.5%, abundant stirring 20-30 minute, rotating speed 800-1200 rev/min (preferred, rotating speed 900-1100 rev/min; Be more preferably, rotating speed 1000 revs/min), evacuation and centrifugal degassing, pours in mould, dry at 50 DEG C, and take off film, soak 1-2 minute with 5% sodium hydrate aqueous solution, then be washed to neutrality, air-dry, sterilizing, obtains biomembrane dressing.
5. the preparation method of Inhalant medical biological film dressing as claimed in claim 4, is characterized in that, step (2) heated and boiled 12-18 minute (preferably, heated and boiled 14-16 minute; Be more preferably, heated and boiled 15 minutes).
6. the preparation method of Inhalant medical biological film dressing as claimed in claim 4, is characterized in that, step (3) was pulverized 140-220 mesh sieve and (preferably, pulverized 160-200 mesh sieve; Be more preferably, pulverized 180 mesh sieves).
7. the preparation method of Inhalant medical biological film dressing as claimed in claim 4, is characterized in that, the 5-7 that the amount of step (3) 80% ethanol is institute's lixiviate raw materials quality is (preferred, 6 times that the amount of 80% ethanol is institute's lixiviate raw materials quality) doubly.
8. the preparation method of Inhalant medical biological film dressing as claimed in claim 4, is characterized in that, step (4) was pulverized 400-800 mesh sieve and (preferably, pulverized 500-600 mesh sieve; Be more preferably, pulverized 700 mesh sieves).
9. the preparation method of Inhalant medical biological film dressing as claimed in claim 4, is characterized in that, step (6) fully stirs 23-27 minute (preferred, fully to stir 25 minutes).
10. the preparation method of Inhalant medical biological film dressing as claimed in claim 4, is characterized in that, volume ratio when each solution of step (6) mixes is chitosan: gelatin: medicinal liquid: glutaraldehyde=1:3:1:0.03.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115721774A (en) * | 2022-10-31 | 2023-03-03 | 博品(上海)生物医药科技有限公司 | Gel based on stem cell extract and preparation method and application thereof |
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| CN104257639A (en) * | 2014-10-15 | 2015-01-07 | 广东璞石医药科技有限公司 | Film coating agent for wound surface treatment and preparation method of film coating agent |
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| US20020054910A1 (en) * | 2000-08-08 | 2002-05-09 | Monteresearch S.R.L. | Topical composition for covering a skin lesion |
| CN1422671A (en) * | 2001-11-23 | 2003-06-11 | 吴天祥 | Medical paste film |
| CN1887358A (en) * | 2006-07-14 | 2007-01-03 | 青岛博益特生物材料有限公司 | Medical chitosan dressing and its application |
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Address after: 810600, Qinghai Province, east city, peace road, No. 11, No. 2, Anju District, 2-901 Applicant after: Geng Wenzhen Address before: 250000, Ji'nan Province, Tian Tian Road, West Road, Shandong, No. 16 Applicant before: Geng Wenzhen |
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Application publication date: 20160217 |