CN105309290A - Cultivation method and application of flue-cured tobacco regenerated plant - Google Patents
Cultivation method and application of flue-cured tobacco regenerated plant Download PDFInfo
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- CN105309290A CN105309290A CN201510668956.3A CN201510668956A CN105309290A CN 105309290 A CN105309290 A CN 105309290A CN 201510668956 A CN201510668956 A CN 201510668956A CN 105309290 A CN105309290 A CN 105309290A
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- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 81
- 241000208125 Nicotiana Species 0.000 title claims abstract description 66
- 241000196324 Embryophyta Species 0.000 title claims abstract description 42
- 238000012364 cultivation method Methods 0.000 title abstract 5
- 238000000034 method Methods 0.000 claims abstract description 22
- 244000061176 Nicotiana tabacum Species 0.000 claims abstract description 15
- 230000008929 regeneration Effects 0.000 claims description 26
- 238000011069 regeneration method Methods 0.000 claims description 26
- 239000011159 matrix material Substances 0.000 claims description 25
- 239000000243 solution Substances 0.000 claims description 22
- 244000037666 field crops Species 0.000 claims description 11
- 238000005286 illumination Methods 0.000 claims description 11
- 230000008774 maternal effect Effects 0.000 claims description 8
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 239000004576 sand Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 239000003337 fertilizer Substances 0.000 claims description 6
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 claims description 5
- 241000238631 Hexapoda Species 0.000 claims description 5
- 238000007689 inspection Methods 0.000 claims description 5
- 230000008635 plant growth Effects 0.000 claims description 5
- 239000012153 distilled water Substances 0.000 claims description 3
- 239000007864 aqueous solution Substances 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- UFWIBTONFRDIAS-UHFFFAOYSA-N naphthalene-acid Natural products C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 239000003643 water by type Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 8
- 238000002791 soaking Methods 0.000 abstract description 7
- 238000004383 yellowing Methods 0.000 abstract description 2
- 239000000758 substrate Substances 0.000 abstract 2
- 230000003044 adaptive effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 230000012010 growth Effects 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000002786 root growth Effects 0.000 abstract 1
- 238000002054 transplantation Methods 0.000 abstract 1
- 238000011534 incubation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 238000004500 asepsis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000000306 component Substances 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0299—Handling or transporting of soil blocks or seedlings
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a cultivation method and application of flue-cured tobacco regenerated plants. The cultivation method of flue-cured tobacco regenerated plants comprises following steps: (1) 45 to 65 days after transplantation of flue-cured tobacco plants to the field, picking off terminal buds or axillary buds of the flue-cured tobacco plants; (2) soaking the picked terminal buds or axillary buds in root growth promoting agent solution; (3)cutting and transplanting the soaked terminal buds or axillary buds to culture substrates and performing cultivation; (4) 25 to 30 days after cutting and transplanting, performing root system checking. If the base part of the terminal buds or axillary buds send forth 8 to 10 adventitious roots, the terminal buds or axillary buds are secondarily transplanted to the field or pots. Then normal management for growth of flue-cured tobacco plants is performed to obtain flue-cured tobacco regenerated plant. According to the cultivation method, terminal buds or axillary buds of flue-cured tobacco plants are adopted and cultured; the components of substrates, light, temperature and humidity are controlled during cultivation process to guarantee the environment is almost identical to adaptive environments of flue-cured tobacco plants. By use of the cultivation method, glass seedlings, yellowing and mottled tobacco are avoided, rooting rate and survival rate are high.
Description
Technical field
The invention belongs to flue-cured tobacco cultivation technical field, be specifically related to a kind of cultural method and application of flue-cured tobacco regeneration plant.
Background technology
Flue-cured tobacco is the important component part of China's tobacco leaf production, is one of important economic crops of China.Improved seeds are important production basis of cured tobacco production development, are to obtain the internal factor of sound tobacco, are volume increase, increase most economical, the most effective measures of matter.Flue-cured tobacco often adopts sexual propagation, but easily morphs, and causes quality deterioration and multiple diseases to occur, loses the characteristic that it is intrinsic.
Plant tissue culture technique is the palingenesis according to plant cell Almightiness type and plant, by a part for sterile working separating plant body, is inoculated on medium, cultivates under manually operated condition, make it produce the process of whole plant.Tissue cultures can keep the excellent present situation of tobacco bred, reduces damage by disease and insect, thus reaches the object of high yield and high quality.In recent years, in the cultivation of flue-cured tobacco and flue-cured tobacco variant, tissue culture technology has played positive role.
Although the operating process of existing flue-cured tobacco Plant Tissue Breeding is also uncomplicated, there is following shortcoming: flue-cured tobacco group training process entails strictly controls the pollution of bacterium, fungi etc., and experimental operating conditions is harsh; In addition, the training of flue-cured tobacco group can cause the generation of the lopsided test-tube plantlet (vitreous shoot) of transparence; The training of flue-cured tobacco group also can cause the whole strain chlorosis of seedling, all or part of yellowing leaf, mottled.
Summary of the invention
The first object of the present invention is the cultural method and the application that provide a kind of flue-cured tobacco regeneration plant, and the second object of the present invention is the application providing described flue-cured tobacco regeneration plant.
The first object of the present invention is achieved in that a kind of cultural method of flue-cured tobacco regeneration plant, comprises the following steps:
Step (1) takes terminal bud or axillalry bud after tobacco plant transplants land for growing field crops 45-65 days;
The terminal bud taken or axillalry bud, at 20 ~ 25 DEG C, are immersed in rooting promoter solution by step (2);
Terminal bud after immersion or axil bud cutting are transplanted in culture matrix and cultivate at 20 ~ 25 DEG C by step (3), and the nutrient solution that rear every milliliter of culture matrix waters 0.13 ~ 0.20mL is transplanted in cuttage, and below ensureing culture matrix, 6 ~ 7cm is moistening.
Step (4) cuttage is transplanted after 25 ~ 30 days and is carried out root system inspection, and terminal bud or axillalry bud base portion grow 8 ~ 10 adventive root, and terminal bud or axillalry bud secondary are transplanted to land for growing field crops or basin alms bowl, by normal tobacco plant growth management, obtain flue-cured tobacco regeneration plant.
The second object of the present invention be achieved in that as described in the flue-cured tobacco regeneration plant that obtains of the cultural method of flue-cured tobacco regeneration plant in the application of the damage by disease and insect Resistance Identification of maternal plant.
Compared with prior art, the present invention has following beneficial effect, the cultural method of flue-cured tobacco regeneration plant provided by the invention, and without the need to carrying out under stringent asepsis requirements, also do not need to use antibiotic, operating process is simple, and rooting rate and survival rate are all higher; The present invention adopts flue-cured tobacco terminal bud or axillalry bud to cultivate, and controls medium component in incubation, illumination, temperature and humidity, ensures almost identical with its suitable environment, has stopped the generation of vitreous shoot, yellow, mottled tobacco leaf.Flue-cured tobacco regeneration plant of the present invention has identical genetic character with maternal plant, keeps the kind of high-quality tobacco; For the damage by disease and insect Resistance Identification of maternal plant, maternal plant yield of tobacco quality determination can not be affected.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but limited the present invention never in any form, and any conversion done based on training centre of the present invention or replacement, all belong to protection scope of the present invention.
Step (1) is selected in tobacco plant transplanting and takes terminal bud or axillalry bud after land for growing field crops 45-65 days, and be in the prosperous long-term to squaring period of tobacco plant this period, the differentiation capability of terminal bud and axillalry bud is strong, can reduce vitrified generation in incubation.
Terminal bud length described in step (1) is 15 ~ 20cm, and axillalry bud length is 8 ~ 10cm.
Rooting promoter solution described in step (2) is naphthalene acid solution, adopts 20 ~ 40% methyl α-naphthyl acetate pulvis and distilled water to mix; The concentration of methyl α-naphthyl acetate is 1.50 ~ 2.00g/L.
Terminal bud or axillalry bud are soaked 1 ~ 2h by step (2) in rooting promoter solution, and terminal bud or axillalry bud are immersed in the degree of depth 2 ~ 4cm in rooting promoter solution.
Described culture matrix is that Friendly tobacco seedling raising medium and river sand mix by weight 1:0.8 ~ 1.2, and described river sand particle diameter is 0.35 ~ 0.5mm, and each composition of this culture matrix is suitable, can reduce incubation Leaf aetiolation.
Described Friendly tobacco seedling raising medium meets YC/T310-2009 standard.
The degree of depth that in step (3), terminal bud or axil bud cutting are transplanted in culture matrix is 3 ~ 5cm; Cuttage transplants latter 1 ~ 7 day, covers the illumination of 70 ~ 80%; Cuttage transplants latter 8 ~ 12 days, covers the illumination of 30 ~ 40%; Cuttage is transplanted after 13 ~ 15 days, adopts natural lighting.
Nutrient solution described in step (3) is the tobacco ternary compound fertilizer special aqueous solution of 1 ~ 5%; N:P in the special ternary compound fertilizer of described tobacco
2o
5: K
2o is 1 ~ 2:1:2 ~ 4.
As preferably, N:P in the special ternary compound fertilizer of described tobacco
2o
5: K
2o is 1:1:2,2:1:4,8:5:17 or 1:1:2.5.
Described flue-cured tobacco regeneration plant is used for the damage by disease and insect Resistance Identification of maternal plant, can not affect maternal plant yield of tobacco quality determination; The genetic character that regeneration plant is identical with maternal plant, keeps the kind of high-quality tobacco, also can collect seed after normally blossoming and bearing fruit.
Below in conjunction with specific embodiment, the invention will be further described.
Following examples rooting promoter solution used adopts 20% methyl α-naphthyl acetate pulvis and distilled water to be made into, and the concentration of methyl α-naphthyl acetate is 1.50-2.00g/L.
Following examples adopt N:P
2o
5: K
2the special ternary compound fertilizer of O=12:12:24 tobacco, be made into running water 1%, 2%, 5% nutrient solution for subsequent use.
embodiment 1
Step (1) carried out taking 100 terminal buds in flue-cured tobacco prosperous long mid-term (flue-cured tobacco transplanting land for growing field crops is after 55 days) to squaring period, and terminal bud length is 20cm;
The terminal bud taken or axillalry bud are soaked 2h, soaking depth 3cm by step (2) in rooting promoter solution at 20 DEG C;
Step (3) fills culture matrix at multiple 5000ml pottery basin or plastic basin, and the terminal bud cuttage of soaking be transplanted in culture matrix and cultivate at 20 ~ 25 DEG C, transplanting depth is 4cm; With hand by culture matrix compacting gently, make culture matrix and terminal bud or axillalry bud base portion close contact, cuttage after transplanting every basin water the nutrient solution of 700mL1%; Described culture matrix is that Friendly tobacco seedling raising medium and river sand mix by weight 1:0.8;
Rear 1-7 days is transplanted in terminal bud cuttage, adopts 2 ~ 3 layers of sunshade net to hide the illumination of removing 70-80%, and rear 8-12 days is transplanted in cuttage, hides the illumination of removing 30-40%, after cuttage transplants 15 days, and employing natural lighting.
Step (4) cuttage is transplanted after 25 days and is carried out root system inspection, terminal bud base portion grows 8 ~ 10 adventive root, add up the ratio (rooting rate) that 100 terminal buds grow 8 ~ 10 adventive root, terminal bud secondary is transplanted to land for growing field crops or basin alms bowl, by normal tobacco plant growth management, obtain flue-cured tobacco regeneration plant, add up its survival rate.
embodiment 2
Step (1) carried out taking 100 axillalry buds in flue-cured tobacco prosperous long mid-term (flue-cured tobacco transplanting land for growing field crops is after 60 days) to squaring period, and axillalry bud length is 9cm;
The axillalry bud taken is soaked 2h, soaking depth 2cm by step (2) in rooting promoter solution at 25 DEG C;
Step (3) fills culture matrix at multiple 5000ml pottery basin or plastic basin, and the terminal bud cuttage of soaking be transplanted in culture matrix and cultivate at 20 ~ 25 DEG C, transplanting depth is 3cm; With hand by culture matrix compacting gently, make culture matrix and terminal bud or axillalry bud base portion close contact, cuttage after transplanting every basin water the nutrient solution of 650mL2%, described culture matrix is that Friendly tobacco seedling raising medium and river sand mix by weight 1:1;
Cuttage was transplanted after 1-7 days, adopted 2 ~ 3 layers of sunshade net to hide the illumination of removing 70-80%, and cuttage is transplanted after 8-12 days, hides the illumination of removing 30-40%, after cuttage transplants 15 days, adopted natural lighting.
Step (4) cuttage is transplanted after 25 days and is carried out root system inspection, terminal bud or axillalry bud base portion grow 8 ~ 10 adventive root, add up the ratio (rooting rate) that 100 axillalry buds grow 8 ~ 10 adventive root, terminal bud or axillalry bud secondary are transplanted to land for growing field crops or basin alms bowl, by normal tobacco plant growth management, obtain flue-cured tobacco regeneration plant, add up its survival rate.
embodiment 3
Step (1) carried out taking 100 terminal buds in flue-cured tobacco prosperous long mid-term (flue-cured tobacco transplanting land for growing field crops is after 45 days) to squaring period, and terminal bud length is 15cm;
The terminal bud taken or axillalry bud are soaked 1h, soaking depth 4cm by step (2) in rooting promoter solution at 25 DEG C;
Step (3) fills culture matrix at multiple 5000ml pottery basin or plastic basin, and the terminal bud cuttage of soaking be transplanted in culture matrix and cultivate at 25 DEG C, transplanting depth is 5cm; With hand by culture matrix compacting gently, make culture matrix and terminal bud or axillalry bud base portion close contact, cuttage after transplanting every basin water 650mL5% nutrient solution, described culture matrix is that Friendly tobacco seedling raising medium and river sand mix by weight 1:1.2;
Axil bud cutting transplants latter 1 ~ 7 day, and adopt 2 ~ 3 layers of sunshade net to hide the illumination of removing 70-80%, cuttage transplants latter 8 ~ 12 days, hides the illumination of removing 30-40%, after cuttage transplants 13 days, and employing natural lighting.
Step (4) cuttage is transplanted after 25 ~ 30 days and is carried out root system inspection, terminal bud or axillalry bud base portion grow 8 ~ 10 adventive root, add up the ratio (rooting rate) that 100 terminal buds grow 8 ~ 10 adventive root, terminal bud or axillalry bud secondary are transplanted to land for growing field crops or basin alms bowl, by normal tobacco plant growth management, obtain flue-cured tobacco regeneration plant, add up its survival rate.
In Statistics Implementation example 1 ~ 3, the ratio of the rooting rate of 100 terminal buds and axillalry bud, survival rate, vitreous shoot and etiolated seedling, as shown in table 1.
Table 1
| Be less than the ratio % of 8 adventive root | Article 8 ~ 10, the ratio % of adventive root | Secondary transplanting survival rate % | Vitreous shoot rate % | Etiolated seedling rate % | |
| Embodiment 1 | 14% | 76% | 91.11 | 0 | 0 |
| Embodiment 2 | 16% | 76% | 95.65 | 0 | 0 |
| Embodiment 3 | 14% | 80% | 93.62 | 0 | 0 |
As seen from Table 1, rooting rate and the secondary transplanting survival rate of the inventive method are all higher, do not have etiolated seedling and vitreous shoot simultaneously.
Claims (9)
1. a cultural method for flue-cured tobacco regeneration plant, is characterized in that, comprises the following steps:
Step (1) takes terminal bud or axillalry bud after tobacco plant transplants land for growing field crops 45-65 days;
The terminal bud taken or axillalry bud, at 20 ~ 25 DEG C, are immersed in rooting promoter solution by step (2);
Terminal bud after immersion or axil bud cutting are transplanted in culture matrix and cultivate at 20 ~ 25 DEG C by step (3), and the nutrient solution that rear every milliliter of culture matrix waters 0.13 ~ 0.20mL is transplanted in cuttage;
Step (4) cuttage is transplanted after 25 ~ 30 days and is carried out root system inspection, and terminal bud or axillalry bud base portion grow 8 ~ 10 adventive root, and terminal bud or axillalry bud secondary are transplanted to land for growing field crops or basin alms bowl, by normal tobacco plant growth management, obtain flue-cured tobacco regeneration plant.
2. the cultural method of flue-cured tobacco regeneration plant as claimed in claim 1, it is characterized in that, the described terminal bud length of step (1) is 15 ~ 20cm, and axillalry bud length is 8 ~ 10cm.
3. the cultural method of flue-cured tobacco regeneration plant as claimed in claim 1, it is characterized in that, the rooting promoter solution described in step (2) is naphthalene acid solution, adopts the methyl α-naphthyl acetate pulvis of 20 ~ 40% and distilled water to mix; The concentration of methyl α-naphthyl acetate is 1.50 ~ 2.00g/L.
4. the cultural method of flue-cured tobacco regeneration plant as claimed in claim 1, it is characterized in that, step (2) terminal bud or axillalry bud soak 1 ~ 2h in rooting promoter solution, and terminal bud or axillalry bud are immersed in the degree of depth 2 ~ 4cm in rooting promoter solution.
5. the cultural method of flue-cured tobacco regeneration plant as claimed in claim 1, is characterized in that, described culture matrix is that Friendly tobacco seedling raising medium and river sand mix by weight 1:0.8 ~ 1.2, and described river sand particle diameter is 0.35 ~ 0.5mm.
6. the cultural method of flue-cured tobacco regeneration plant as claimed in claim 1, is characterized in that, the degree of depth that terminal bud or axil bud cutting are transplanted in culture matrix is 3 ~ 5cm.
7. the cultural method of flue-cured tobacco regeneration plant as claimed in claim 1, it is characterized in that, the nutrient solution described in step (3) is the tobacco ternary compound fertilizer special aqueous solution of 1 ~ 5%; N:P in the special ternary compound fertilizer of described tobacco
2o
5: K
2o is 1 ~ 2:1:2 ~ 4.
8. the cultural method of flue-cured tobacco regeneration plant as claimed in claim 1, is characterized in that, cuttage transplants latter 1 ~ 7 day, covers the illumination of 70 ~ 80%; Cuttage transplants latter 8 ~ 12 days, covers the illumination of 30 ~ 40%; Cuttage is transplanted after 13 ~ 15 days, adopts natural lighting.
9. one kind as arbitrary in claim 1 to 9 as described in the flue-cured tobacco regeneration plant that obtains of the cultural method of flue-cured tobacco regeneration plant in the application of the damage by disease and insect Resistance Identification of maternal plant.
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|---|---|---|---|
| CN201510668956.3A CN105309290B (en) | 2015-10-13 | 2015-10-13 | A kind of cultural method of flue-cured tobacco regeneration plant and application |
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|---|---|---|---|
| CN201510668956.3A CN105309290B (en) | 2015-10-13 | 2015-10-13 | A kind of cultural method of flue-cured tobacco regeneration plant and application |
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| CN105309290A true CN105309290A (en) | 2016-02-10 |
| CN105309290B CN105309290B (en) | 2017-12-08 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1452660A (en) * | 2000-08-11 | 2003-10-29 | 辛根塔参与股份公司 | Methods for stable transformation of plants |
| CN103109745A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Method for removing tobacco mosaic virus and rapidly cultivating non-toxic seedling in test tube |
| CN103168687A (en) * | 2013-03-22 | 2013-06-26 | 云南省烟草农业科学研究院 | Method for inducing fast rooting of cluster buds of tobacco leaves |
| CN104782455A (en) * | 2014-01-17 | 2015-07-22 | 广东省农业科学院作物研究所 | Matrix for tobacco axillary bud cutting propagation and application thereof |
-
2015
- 2015-10-13 CN CN201510668956.3A patent/CN105309290B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1452660A (en) * | 2000-08-11 | 2003-10-29 | 辛根塔参与股份公司 | Methods for stable transformation of plants |
| CN103109745A (en) * | 2013-03-10 | 2013-05-22 | 通化师范学院 | Method for removing tobacco mosaic virus and rapidly cultivating non-toxic seedling in test tube |
| CN103168687A (en) * | 2013-03-22 | 2013-06-26 | 云南省烟草农业科学研究院 | Method for inducing fast rooting of cluster buds of tobacco leaves |
| CN104782455A (en) * | 2014-01-17 | 2015-07-22 | 广东省农业科学院作物研究所 | Matrix for tobacco axillary bud cutting propagation and application thereof |
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| Title |
|---|
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