CN105238779B - SNP marker and its application - Google Patents
SNP marker and its application Download PDFInfo
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- CN105238779B CN105238779B CN201410329331.XA CN201410329331A CN105238779B CN 105238779 B CN105238779 B CN 105238779B CN 201410329331 A CN201410329331 A CN 201410329331A CN 105238779 B CN105238779 B CN 105238779B
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Abstract
The invention discloses SNP marker and its applications.Wherein, which is the base C or T of No. 26 5119569 positions of chromosome of goat.The SNP marker of the present invention and the wool crimping character of goat are closely related, can be effective for the molecular mark of goat.
Description
Technical field
The present invention relates to SNP marker and its applications.In particular it relates to the relevant SNP of goat wool crimping character
Label, for detecting the primer pair of aforementioned SNP marker and kit, aforementioned SNP marker, primer pair, kit are in goat selection and breeding
In purposes and detect goat wool crimping character method.
Background technology
China is the country that Goats Breeds resource is the abundantest in the world, can be divided into suede use, hair according to goat purposes difference
With, Qiu Yong, lambskin, meat and milk is with goat etc..And goats hair is generally divided into inside and outside two layers.Internal layer is by the secondary hair in skin
Capsule formed without marrow wool fibre, cashmere is thin and soft, and gloss is good, and thermal property is strong, is known as the laudatory title of " soft gold ", usually
The foreign exchange earning textile material important as China.Guard hairs is grown by primary follicle to be formed, compared with sheep's wool, bending
It is few.Goats hair is mainly used for woven carpet, woollen blanket, various tweed material, writing brush, tent etc..By largely being processed to various wools
Result it is for statistical analysis, finding the crimpness of wool fiber, there is certain to fibre property and yarn, fabric property
It influences.During carpet weaving, in general crushing resistance and feel can be improved using curling hair.But the curling of wool is sometimes
It is that unfavorable, particularly excessive crimpness is unfavorable to spinning processing and products thereof to processing.The increase of wool fibre crimpness can increase
The fracture of fiber and fuds fuddled, crimpness during carding is added also to influence the drafting force in coalescence process.Thus, in production cultivation
Need the Goats Breeds according to actual demand cultivation wool crimping degree different (frizzle or broad wool).But traditional selection approach
It is difficult to obtain effective progress.Molecular marker assisted selection, can be by influencing to select time, selection intensity and accuracy and pole
The earth improves the selection effect of this kind of character.
Have at present using wide molecular genetic marker technique：Restriction fragment length technology
(Restriction Fragment Length Polymorphism, RFLP), randomly amplified polymorphism DNA technique
(Random Amplified Polymorphism DNA, RAPD), amplified fragment length polymorphism technology (Amplified
Fragment Length Polymorphism, AFLP), single nucleotide polymorphism (single nucleotide
Polymorphism, SNP) etc..Wherein, SNP marker have many advantages, such as inheritance stability, mutation rate it is low, convenient for automatic detection, because
This exploitation will generate the goat new varieties (being) of wool crimping needed for cultivation with the relevant SNP genetic markers of goat wool crimping
Significant impact.Wherein, it is based on about the research of the genetic marker of goat wool crimping at present in other kinds or species more
It was found that hair waving candidate gene.These wool crimping correlations found in the research of goat wool crimping genetic mechanism are lost
Pass label it is mostly be it is preliminary as a result, and be confined to have candidate gene, using these as a result, being also difficult to goat wool crimping
Character makes accurate selection.Thus, at present, excavated and the relevant genetic marker of wool crimping character in full genome level
Still having must have very much.
Invention content
The present invention is directed at least solve one of technical problem in the prior art.For this purpose, one object of the present invention
It is to propose that one kind is related to goat wool crimping character, it can be effective for the SNP marker of goat selection and breeding.
Wherein, it should be noted that (single nucleotide polymorphism, SNP, i.e. mononucleotide are more by SNP
State property) it is that the molecule that the human genome research center scholar Lander by Massachusetts Institute Technology in 1996 is proposed is lost
Label is passed, is primarily referred to as DNA sequence polymorphism caused by a single nucleotide variation in genomic level.SNP is shown
Polymorphism relate only to the variation of single base, performance is that have conversion, transversion, insertion and missing etc..
According to an aspect of the present invention, the present invention provides a kind of relevant SNP markers of goat wool crimping character.Root
According to the embodiment of the present invention, the SNP marker is the base C or T of No. 26 5119569 positions of chromosome of goat.
According to an embodiment of the invention, the SNP marker is located at SEQ ID NO：The box label of nucleotide sequence shown in 1
Place：
TACTTGAGGCATAGATTGTAGATAACTTTTTTGAAAAAGTGTTTGCTCCTGTGAGAAAGTTAGACTGTTATCGTTAA
AAATCAAATTCACAGTTGCGGTTCTTCTGTTTATTCAGGTAGAATTCAGGACTACTGAATTTACAAATTGATAACAT
TATGATTTATCCTTGAGCTGAAGAAATCAGTGATGTGTGAATAAAGGTTGCAAATGCTGATGAAGGCTTGTGTTGGT
TATAGATTGAGTCTCAATAAAGTTTGGGTTTCTTTGCTTTCTGTTTTTTCTCCACTGCATTCAACATCAAGGTTTAA
CAAGGCAGTTTTCTTTTTACTTTTGGGTGGCAGGGCAGTGGTGGCTGGAAAGGGGAAATAAATTCTCATTCACCTTT
AAAATTCACAACCCCATGGACTGCAGCATGCCAAGCCTTCCTGTCCATCACCAACTCCCAGAGATTATGCAGACTCA
TGTTCATCGAGTTAATGATGCCATCCAGCCACCTCATCCACCTTTAGCATTCTTCAGATTGGAGTCTTATTAGAC
TTACATCTATGAGTGGGCCTTGATCTTGCCTCCTATTCTCTGAGCCACTCAAGACCTCATAAATTAAATTTTAAGTT
CACTTGGATCAACAGAAACCCTATAAGTGAACTTACTGTCAGTACTAAACTGTCTAGATCCTTGTTTTCATTTAGTT
TTTGACCTTCATACTTATTTTCTTCTTATCTTCTTGATATGTTTAGGAAAAATTATTAAGATCCTTTCAGTTCAGTC
AGT(SEQ ID NO：1).
According to an embodiment of the invention, broad wool individual proportion is significantly higher than TT in CC, CT genotype individuals of the SNP site
The broad wool individual proportion of genotype.CC the and CT genotype of the SNP site can be as judging the straight frizzle character of goat as a result,
Major criterion.
I.e. inventor has found, broad wool individual proportion pole is significantly higher than TT genes in CC, CT genotype individuals of the SNP site
The broad wool individual proportion (p=0.000) of type.And then show that CC the and CT genotype of the SNP site can be used as and judge that goat is straight
The major criterion of frizzle character.It and then according to an embodiment of the invention, can be effectively pre- by detecting the above-mentioned SNP of goat
Its wool crimping character is surveyed, specifically, as previously mentioned, broad wool individual proportion is notable in CC, CT genotype individuals of the SNP site
Higher than the broad wool individual proportion of TT genotype.So as to, for example, the SNP marker site genotype be CC and/or CT when, then can
Enough predict goat to be measured very it is big may be broad wool individual.Inventor determines as a result, the wool of SNP marker of the invention and goat
Curling character is closely related, can be effective for the molecular mark of goat.It and then can be according to practical breeding demand
Seedling selection (such as selection broad wool goat individual) is carried out to Goat Breeding material, is further able to effectively improve the efficiency of breeding
And accuracy, the genetic level of raising Goat Reproduction group, so as to accurately and efficiently select goat improved seeds.This
Outside, according to some embodiments of the present invention, goat molecule marker-assisted breeding is carried out using the SNP marker of the present invention, had early
The advantages of phase screens, saves the time, is of low cost, accuracy is high.
According to another aspect of the present invention, the present invention also provides a kind of for detecting one kind of the foregoing present invention
The primer pair of SNP marker.According to an embodiment of the invention, the primer pair includes：With SEQ ID NO：Nucleosides shown in 2-3
Acid sequence, for detecting the SNP marker.
Specifically, the sequence of primer pair is as follows：
F：CAAGGTTTAACAAGGCAGTT(SEQ ID NO：2)
R：CTGTTGATCCAAGTGAACTT(SEQ ID NO：3)
It according to an embodiment of the invention, can be effectively to the above-mentioned and wool of goat to be measured using the primer pair of the present invention
Segment where crimping the relevant SNP marker of character carries out PCR amplification, and then can effectively be realized to this SNP by sequencing
The detection of label, determines the genotype in this kind of SNP marker site of goat to be measured, and then can effectively predict the wool of goat to be measured
Crimp character.Specifically, broad wool individual proportion is significantly higher than the straight of TT genotype in CC, CT genotype individuals of the SNP site
The individual proportion of hair.So as to, for example, the SNP marker site genotype be CC and/or CT when, then can predict goat pole to be measured
It may be broad wool individual.It, being capable of effective Yushan Hill as a result, for detecting the primer pair of the SNP marker of the foregoing present invention
The molecular mark of sheep, so can assist early stage realize the short time, low cost, high accuracy selection and breeding goat it is excellent
Kind.
According to another aspect of the invention, the present invention also provides a kind of for detecting the examination of foregoing SNP marker
Agent box.According to an embodiment of the invention, which includes：It is described previously for the primer of the SNP marker of the detection present invention
It is right.Being included in kit of the invention has SEQ ID NO：The primer pair of nucleotide sequence shown in 2-3.According to the present invention
Embodiment, using the present invention kit included in primer pair, can effectively realize the above-mentioned and sheep to goat to be measured
The polymorphic detection of the relevant SNP markers of wadding of wool Qu Xingzhuan determines the genotype in the goat to be measured SNP marker site, Jin Erneng
Enough wool crimping characters for effectively predicting goat to be measured.Specifically, broad wool individual in CC, CT genotype individuals of the SNP site
Proportion is significantly higher than the broad wool individual proportion of TT genotype.So as to, for example, the SNP marker site genotype for CC and (or)
During CT, then can predict goat to be measured very it is big may be broad wool individual.It is of the invention for detecting foregoing hair as a result,
The kit of bright SNP marker, can be effective for the molecular mark of goat, and then it is short that early stage can be assisted to realize
Time, low cost, high accuracy ground selection and breeding goat improved seeds.
In accordance with a further aspect of the present invention, the present invention also provides the SNP marker of the foregoing present invention, primer pair or
Kit, the purposes in goat selection and breeding.As previously mentioned, by can be used in detection the present invention with goat wool crimping character
The reagent example primer pair as the aforementioned of relevant SNP marker or the kit comprising the primer pair etc. can be effectively detected really
The genotype of the above-mentioned SNP marker of fixed goat to be measured, and then the genotype based on acquisition can effectively predict the sheep of goat to be measured
Wadding of wool Qu Xingzhuan, so as to effectively assist goat selection and breeding.
And then according to another aspect of the present invention, the present invention also provides a kind of sides for detecting goat wool crimping character
Method.According to an embodiment of the invention, this method predicts institute by the detection to the foregoing SNP marker of goat to be measured progress
State the wool crimping character of goat to be measured.Specifically, can by can be used in detection the present invention with goat wool crimping
The reagent example primer pair as the aforementioned of the relevant SNP marker of shape or the kit comprising the primer pair etc. carry out goat to be measured
PCR amplification, sequencing, to detect the genotype of the above-mentioned SNP marker of determining goat to be measured, and then the genotype energy based on acquisition
Enough wool crimping characters for effectively predicting goat to be measured.Wherein, it is as previously mentioned, straight in CC, CT genotype individuals of the SNP site
The individual proportion of hair is significantly higher than the broad wool individual proportion of TT genotype.So as to, such as the genotype in the SNP marker site is CC
And (or) during CT, then it is most probably broad wool individual that can predict goat to be measured.Detection goat wool crimping of the invention as a result,
The method of shape can quickly, efficiently and accurately detect goat wool crimping character, and then can be effective for the molecule of goat
Marker-assisted breeding, so as to which early stage is assisted to realize short time, low cost, high accuracy ground selection and breeding goat improved seeds.
In addition, the method for detection goat wool crimping character according to the above embodiment of the present invention can also be with following attached
The technical characteristic added：
According to an embodiment of the invention, the method for carrying out SNP marker detection to goat to be measured is not particularly limited.Sequencing,
Single-strand conformation polymorphism PCR (PCR single strand conformation polymorphism, PCR-
SSCP), restriction fragment length polymorphism PCR (PCR-restriction fragment length
Polymorphism, PCR-RFLP) and the technologies such as flight time mass spectrum can realize the detection of SNP.Wherein, sequencing is a kind of
Accuracy highest, flexibility are strong, the detection technique that flux is big, detection cycle is short.A pair only need to be designed in the both sides of SNP site to draw
Object expands the product of 400-700bp, then the genotype of SNP site can be directly detected by sequencing.Thus, the present invention uses
The method of sequencing carries out SNP marker detection.Some specific examples according to the present invention, it is noted earlier by being carried out to goat to be measured
The detection of SNP marker is predicted the wool crimping character of the goat to be measured, is further comprised：Extract the genome of goat to be measured
DNA；Using foregoing primer pair, the genomic DNA of the goat to be measured is subjected to PCR amplification, to obtain PCR amplification
Product；The pcr amplification product is sequenced, to obtain sequencing result；Based on the sequencing result, determine described to be measured
The genotype of the SNP marker of goat；And the genotype of the SNP marker based on the goat to be measured, described in prediction
The wool crimping character of goat to be measured.Thereby, it is possible to effectively improve the efficiency of detection goat wool crimping character.
According to an embodiment of the invention, the method for extracting the genomic DNA of goat to be measured is not particularly limited, and may be used
Any of genome DNA extracting method or kit carry out.Some specific examples according to the present invention, using conventional phenol-
Chloroform method extracts the genomic DNA of goat to be measured.Thereby, it is possible to effectively obtain genomic DNA high-quality, that purity is high, just
It is carried out in subsequent step.
According to an embodiment of the invention, the genomic DNA of the goat to be measured is subjected to the condition of PCR amplification not by special
Limitation.Some specific examples according to the present invention, the amplification system of the PCR amplification are calculated as with 20 μ l：The template of 25-50ng/ μ l
The SEQ ID NO of DNA2 μ l, 10pmol/ μ l：DNTP mix 0.5 the μ l, 5U/ of primer each 0.3 μ l, 10mmol/L shown in 2-3
0.2 μ l, 10 × PCR reaction buffer of Taq archaeal dna polymerases, the 2 μ l of μ l, surplus is distilled water；The reaction condition of the PCR amplification
For：94 DEG C 5 minutes；94 DEG C 30 seconds, 56 DEG C 30 seconds, 72 DEG C 30 seconds, 35 cycle；72 DEG C 5 minutes.Thereby, it is possible to fast
Speed, efficiently and accurately expand the present invention SNP marker where segment, obtain target amplification product, convenient for subsequent step into
Row.
According to an embodiment of the invention, the method pcr amplification product being sequenced is not particularly limited, as long as energy
The sequence of segment where enough effectively acquisition pcr amplification product, that is, SNP markers.Some specific examples according to the present invention,
May be used selected from HISEQ2000, SOLiD, 454 and single-molecule sequencing method it is at least one to the pcr amplification product into
Row sequencing.Thereby, it is possible to it is high-throughput, quick, efficiently and accurately obtain sequencing result.
It according to an embodiment of the invention,, can effectively really by comparing goat reference gene group sequence based on sequencing result
The SNP marker of fixed goat to be measured is CC, TT or CT.
According to an embodiment of the invention, broad wool individual proportion pole is significantly higher than in CC, CT genotype individuals of the SNP site
The broad wool individual proportion (p=0.000) of TT genotype.As a result, based on the genotype of the SNP marker of determining goat to be measured,
It can accurately and effectively predict the wool crimping character of goat to be measured.Specifically, such as the genotype in the SNP marker site is
During CC and (or) CT, then can predict goat to be measured very it is big may be broad wool individual.And then the method for the present invention can be effective
In the molecular mark of goat, so as to which early stage is assisted to realize short time, low cost, high accuracy ground selection and breeding goat
Improved seeds.
It should be noted that the SNP marker relevant with goat wool crimping of the present invention and its application have the following advantages that：
(1) SNP marker provided by the invention is by limitations such as the age of goat, genders, available for the early stage selection and breeding of goat,
The breeding process of goat can be remarkably promoted；
(2) method of the goat SNP site of the detection present invention, it is accurately and reliably, easy to operate；
(3) detection of goat SNP site of the invention, the marker assisted selection for goat wool crimping character provide section
Learn foundation.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
It obtains significantly or is recognized by the practice of the present invention.
Description of the drawings
The above-mentioned and additional aspect and advantage of the present invention will become bright in the description from combination accompanying drawings below to embodiment
It shows and is readily appreciated that, wherein：
Fig. 1 shown according to one embodiment of the invention, the Manhattan figure in SNP marker site of the invention.
Fig. 2 shown according to one embodiment of the invention, the agarose gel electrophoresis figure of SNP site of the invention.
Fig. 3 shows that according to one embodiment of the invention peak figure is sequenced in CC, CT genotype of SNP site of the invention,
In,
Fig. 3 a are that peak figure is sequenced in the CC genotype of the SNP site；
Fig. 3 b are that peak figure is sequenced in the CT genotype of the SNP site.
Specific embodiment
The embodiment of the present invention is described below in detail.The embodiments described below with reference to the accompanying drawings are exemplary, only
For explaining the present invention, and it is not considered as limiting the invention.
The acquisition of embodiment 1 and the relevant SNP marker of goat wool crimping
Inventor excavates Grey Goats wool crimping correlated inheritance mark by RAD-seq and whole-genome association (GWAS)
Note can improve the accuracy of wool crimping character determination using these labels.It is as follows：
1st, the acquisition of Grey Goats blood sample and the extraction of DNA
1) 457 parts of blood samples pick up from Shandong Yong Wang herdings Development Co., Ltd Grey Goats, wool crimping character statistics
Such as table 1.
2) genomic DNA is extracted using phenol-chloroform method.
1 Grey Goats wool crimping character statistical result of table
| Character | Broad wool | Frizzle | It is total |
| Number of individuals | 431 | 26 | 457 |
2nd, RAD-seq library constructions
Library constructing method refers to (Rapid SNP Discovery and Genetic Mapping Using
Sequenced RAD Markers), it is specific as follows：
1) digestion, connection P1 connectors (containing barcode) are carried out to genome with Taq I；
2) it interrupts at random, connects P2 connectors；
3) sequence simultaneous with P1 and P2 connectors is screened by PCR；
4) segment for choosing 400bp-700bp is sequenced, wherein, each sample mean obtains the data of 1.2G, average
Sequencing depth 15 ×.
3rd, whole-genome association
Whole-genome association (GWAS) is carried out using Plink softwares, 1 and goat are filtered out from 140,000 SNP
Straight frizzle character closely significantly correlated SNP, specifying information such as Fig. 1 and table 2.
Fig. 1 shows the Manhattan figure in the SNP marker site.As shown in Figure 1, abscissa is chromosome numbers, ordinate
For association analysis-log P values, dotted line is 5% significant threshold line (6.6) of genomic level, single nucleotide polymorphism label
Color corresponds to specific chromosome.
The statistical information of 2 SNP site of table
| Chromosome | Positiona | Alleleb | Minimum gene frequency | Pc |
| Chr26 | 5119569 | C/T | 0.037 | 3.10×10-10 |
Note：A. BLAST is carried out with the SNP flanking sequences that RAD-seq is obtained, is located in genome (Capra
hircus CHIR_1.0)。
B. there is 51 idiotypes missing in the site.C. the P values obtained in association analysis with chi-square criterion.
Shown as the whole-genome association result shown in Fig. 1, table 2 and statistical information：The SNP site is directly rolled up with goat
Extremely significantly correlated (p=3.10 × 10 of hair character-10).And then show SNP site to judge that goat wool directly rolls up relevant SNP marks
Note.
4th, SNP site genotype and the statistics of wool crimping number of individuals
The individual genotype of the SNP site of 457 parts of Grey Goats and phenotype statistical information such as table 3, and straight to different genotype
Hair probability of occurrence has carried out t inspections, as a result shows significant difference (p ＜ 0.05).
The SNP site genotype of table 3 and the information of individual wool crimping
Statistical information shown in table 3 shows：Broad wool individual proportion is extremely notable in CC, CT genotype individuals of the SNP site
Higher than the broad wool individual proportion (p=0.000) of TT genotype.And then show that CC the and CT genotype of the SNP site can conduct
Judge the major criterion of the straight frizzle character of goat.
The sequence verification of embodiment 2 and the relevant SNP marker of goat wool crimping
Genomic DNA in 2.1 extraction Grey Goats blood samples to be measured
Grey Goats blood sample to be measured comes from Shandong Han Longyang industry limited company Grey Goats, randomly selects 5 parts, according to
DNA extraction method extracting genomic DNA described in embodiment 1.
2.2 nucleotide fragments of the amplification containing SNP site
Genomic DNA in each Grey Goats blood sample to be measured obtained using aforementioned extraction utilizes forward primer as template
F：CAAGGTTTAACAAGGCAGTT(SEQ ID NO：And reverse primer R 2)：CTGTTGATCCAAGTGAACTT(SEQ ID
NO：3) nucleotide fragments where SNP to be measured, are amplified.The amplification system of the PCR amplification is calculated as with 20 μ l：25-50ng/μl
2 μ l, 10pmol/ μ l of template DNA SEQ ID NO：The dNTP mix 0.5 of primer each 0.3 μ l, 10mmol/L shown in 2-3
0.2 μ l, 10 × PCR reaction buffer of Taq archaeal dna polymerases, the 2 μ l of μ l, 5U/ μ l, surplus is distilled water；The PCR amplification it is anti-
The condition is answered to be：94 DEG C 5 minutes；94 DEG C 30 seconds, 56 DEG C 30 seconds, 72 DEG C 30 seconds, 35 cycle；72 DEG C 5 minutes.
2.3 sequencing identification SNP site genotype
For PCR product obtained in abovementioned steps first after 1.5% agarose gel electrophoresis detection, result is single spy
(see Fig. 2, as shown in Fig. 2, left side is electrophoresis result figure, target fragment 270bp, right side is corresponding after different in nature band
Marker schemes), it is unidirectionally sequenced on ABI3730 sequenators, identification SEQ ID NO：(i.e. this hair at 501bp in 1 sequence
Bright SNP marker) genotype.Wherein, the sequencing peak figure of two kinds of genotype of CC and CT is respectively as shown in Fig. 3 a, 3b.Wherein, such as
Shown in Fig. 3, when sequencing, employs reverse primer sequences, so the sequence measured is reverse complementary sequence.
In the description of this specification, reference term " one embodiment ", " example ", " is specifically shown " some embodiments "
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment of the present invention or example.In the present specification, schematic expression of the above terms are not
Centainly refer to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be any
One or more embodiments or example in combine in an appropriate manner.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not
In the case of being detached from the principle of the present invention and objective a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The range of invention is limited by claim and its equivalent.
Claims (8)
1. a kind of relevant SNP marker of goat wool crimping character, which is characterized in that
The SNP marker is base C or T, and the SNP marker is located at No. 26 chromosomes of goat, and position is the 501st of nucleic acid,
The nucleic acid has SEQ ID NO:Nucleotide sequence shown in 1.
2. SNP marker according to claim 1, which is characterized in that broad wool in CC, CT genotype individuals of the SNP site
Individual proportion is significantly higher than the broad wool individual proportion of TT genotype.
3. a kind of primer pair that the SNP marker described in 1 or 2 is required for test right, which is characterized in that the primer pair has
SEQ ID NO：Nucleotide sequence shown in 2-3, for detecting the SNP marker.
4. a kind of kit that the SNP marker described in 1 or 2 is required for test right, which is characterized in that include：
Primer pair described in claim 3.
5. the kit described in the primer pair or claim 4 described in SNP marker, claim 3 described in claims 1 or 2,
Purposes in goat selection and breeding.
6. it is a kind of detect goat wool crimping character method, which is characterized in that by goat to be measured carry out claim 1 or
The wool crimping character of the goat to be measured is predicted in the detection of SNP marker described in 2.
7. according to the method described in claim 6, it is characterized in that, by being carried out described in claims 1 or 2 to goat to be measured
The detection of SNP marker is predicted the wool crimping character of the goat to be measured, is further comprised：
Extract the genomic DNA of goat to be measured；
Using the primer pair described in claim 3, the genomic DNA of the goat to be measured is subjected to PCR amplification, to obtain
Pcr amplification product；
The pcr amplification product is sequenced, to obtain sequencing result；
Based on the sequencing result, the genotype of the SNP marker of the goat to be measured is determined；And
The genotype of the SNP marker based on the goat to be measured predicts the wool crimping character of the goat to be measured.
8. the method according to the description of claim 7 is characterized in that broad wool individual in CC, CT genotype individuals of the SNP site
Proportion is significantly higher than the broad wool individual proportion of TT genotype.
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| CN107460232A (en) * | 2016-06-06 | 2017-12-12 | 西南大学 | One molecular labeling related to goat hair color |
| CN107630095B (en) * | 2017-10-23 | 2021-03-05 | 新疆畜牧科学院畜牧研究所 | Molecular marker related to sheep wool bending number character and specific primer pair and application thereof |
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| CN1263559A (en) * | 1997-05-30 | 2000-08-16 | 猪改良英国有限公司 | Method for analyzing animal products |
| WO2005052133A3 (en) * | 2003-11-24 | 2006-03-09 | Mmi Genomics Inc | Method and markers for determining the genotype of horned/polled cattle |
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