CN105210874B - A kind of blueberry method for culturing seedlings - Google Patents

Abstract

The present invention relates to blueberry seedling-raising technique fields, especially a kind of blueberry method for culturing seedlings, by by semi-lignified branch segment, detergent immersion, sodium hypochlorite impregnate, mercuric chloride solution impregnates, flushing is seeded in WPM culture mediums repeatedly, and carry out improved treatment to WPM culture mediums so that the interaction between its nutritional ingredient is adjusted, and then while nutritional ingredient is provided in for blueberry seedling raising process, also promote the growth of blueberry twig and improving quality, improve premunition, reduce blueberry seedling cost；In conjunction with being cultivated in switching root media so that blueberry root system is quickly flourishing, and ensures the survival rate of follow-up blueberry transplanting, reduces production cost.

Description

A kind of blueberry seedling raising method

technical field

The invention relates to the technical field of blueberry seedling cultivation, in particular to a blueberry seedling cultivation method.

Background technique

Blueberry (Blueberry), means the meaning of blue berry. According to the different cultivation methods and the different growth environments of blueberries, the characteristics they present are also different, and they are further divided into two types, one is low shrub, short-footed wild, small particles, but the content of anthocyanins The tall variety, the other is artificially bred, can grow to 240cm high, has larger fruit, more water, and a relatively low anthocyanin content. Blueberry fruit is rich in nutrients. It not only has good nutritional and health effects, but also has the functions of preventing brain aging, strengthening the heart, anti-cancer, softening blood vessels, and enhancing human immunity. Especially the anthocyanins in blueberries, which can resist aging, resist oxidation and improve human immunity.

But, along with the continuous increase of the demand of blueberry and the continuous development of industry, also more and more for the demand of blueberry, for this reason, the supply of wild blueberry can no longer meet the demand of blueberry industry, and artificially cultivated blueberry and It will cause a significant reduction in the anthocyanin content in blueberries, which in turn will affect the healthy development of the blueberry industry.

Therefore, how to provide a kind of artificial seedling cultivation and reduce the defects of blueberry variety variation has become one of the restrictive factors for the rapid and healthy development of the blueberry industry. And in the prior art, a large amount of researchers have also been engaged in the research and processing of blueberry seedling raising method, and also obtained certain breakthrough and development, but, the quality of the blueberry seedling that it cultivates is still relatively poor, and the degree of variation is still relatively high. Large, and its cultivation cycle is relatively long, the survival rate of transplanted blueberry seedlings after cultivation is low, which makes the cost of blueberry planting higher and hinders the rapid and healthy development of the blueberry industry.

Contents of the invention

In order to solve the above-mentioned technical problems existing in the prior art, the present invention provides a method for growing blueberry seedlings.

Specifically, it is achieved through the following technical solutions:

A method for raising blueberry seedlings, comprising the following steps:

(1) Select semi-lignified branches, cut them into 1-1.5cm single-bud branches, soak them in saturated washing powder water for 9-11 minutes, then rinse them with tap water for 9-11 minutes, and then use quality Soak in 70% sodium hypochlorite for 4-6 minutes, then soak in 0.1% mercuric chloride solution for 7-9 minutes, then rinse with sterile water for 5-6 times, then inoculate it on WPM medium for cultivation , the control temperature is 24-26°C, the light intensity is 1900-2100LX, and the light time is 15-17h/day;

(2) After step 1) is cultivated until the plant grows to a plant height of 6-8cm, it is transferred to the rooting medium for rooting treatment.

The rooting treatment is the rooting culture of cuttings outside the bottle or the rooting culture in the bottle.

The rooting culture of cuttings outside the bottle is to cut the plants with a plant height of 6-8cm into cutting strips of 3-5cm, and cut the cutting strips on the rooting medium, the cutting depth is 1-1.5cm, and cut the cutting strips on the rooting medium. Use IBA with a mass volume concentration of 0.1mg/L before dipping, and after cuttings, control the temperature at 24-26°C, light intensity 1000-2000K, light time 15-17h/day, and cultivate for two months. .

The cultivation rooting in described bottle is that the plant height of 6-8cm is transferred in the rooting medium, wherein the rooting medium is contained in a jar, and its rooting medium is 1/2WPM medium adding IBA, Make the concentration of IBA in the culture medium 0.1mg/L, then place it at a temperature of 24-26°C, light intensity of 1000-2000K, light time of 15-17h/day, and cultivate for two months.

The rooting medium is a rooting medium for cuttings outside a bottle, which is composed of peat soil and bark powder uniformly mixed in a mass ratio of 3:1.

Described rooting medium is culture rooting medium in the bottle, and this medium is 1/2WPM medium and adds IBA, makes the concentration of IBA in the medium be 0.1mg/L.

Described WPM culture medium, by every liter, its raw material composition is ammonium nitrate 400mg, calcium nitrate 556mg, potassium sulfate 990mg, calcium chloride 96mg, potassium dihydrogen phosphate 170mg, sodium molybdate 0.25mg, magnesium sulfate 370mg, sulfate Manganese 22.4mg, Zinc Sulfate 8.6mg, Copper Sulfate 0.25mg, Ferrous Sulfate 27.8mg, Na ₂ -EDTA 37.3mg, Inositol 100mg, Niacin 0.5mg, Vitamin B6 0.5mg, Glycine 2.0mg, Sucrose 20g, Agar 6g , Zeatin 0.5 ~ 1mg.

The sterile water is distilled water or tap water boiled at 80-100° C. for 10-30 minutes and then cooled to normal temperature.

Compared with the existing technology, its technical effect is reflected in:

By cutting the semi-lignified branches, soaking them in washing powder, sodium hypochlorite, mercuric solution, washing repeatedly and inoculating them on the WPM medium, and improving the WPM medium, the interaction between the nutrients can be adjusted. Furthermore, while providing nutrients for the blueberry seedling cultivation process, it also promotes the growth of blueberry shoots and improves the quality, improves disease resistance, and reduces the cost of blueberry seedling cultivation; combined with the cultivation in the transfer rooting medium, the blueberry root system develops rapidly. And the survival rate of subsequent blueberry transplanting is ensured, and the production cost is reduced.

In particular, the present invention carries out the cultivation of the blueberry root system and the cultivation of the blueberry seedling plant body through different mediums, thereby ensuring that the nutrients required for root growth are not affected by the nutrients required for plant body growth, thereby improving the growth of blueberry seedlings environment, improve the quality of blueberries, and then reduce the incidence of blueberries; especially through the cultivation of rooting medium, not only makes the blueberry root system developed, the disease resistance is enhanced, but also makes the blueberry seedlings aging and mature, thereby improving the ability to adapt to the environment, Reduce the loss rate of transplanting and improve the survival rate.

Detailed ways

The technical solutions of the present invention will be further limited below in conjunction with specific embodiments, but the scope of protection is not limited to the descriptions made.

Example 1

A method for raising blueberry seedlings, comprising the following steps:

(1) Select semi-lignified branches, cut them into 1cm single-bud branches, soak them in saturated washing powder water for 9 minutes, then rinse them with tap water for 9 minutes, and then soak them in 70% sodium hypochlorite under aseptic conditions. After 4 minutes, soak in mercuric chloride solution with a mass concentration of 0.1% for 7 minutes, rinse repeatedly with sterile water for 5 times, and then inoculate it on WPM medium for cultivation. The time is 15h/day;

(2) After the step 1) is cultivated until the plant grows to a plant height of 6 cm, it is transferred to the rooting medium for rooting treatment.

The rooting treatment is rooting culture of cuttings outside the bottle.

The rooting culture of cuttings outside the bottle is to cut the plants with a plant height of 6cm into 3cm cutting strips, and cut the cutting strips on the rooting medium, the cutting depth is 1cm, and the mass volume concentration is 0.1 before cutting. mg/L IBA, and after cuttings, control the temperature at 24°C, light intensity 1000K, light time 15h/day, and cultivate for two months.

The rooting medium is a rooting medium for cuttings outside a bottle, which is composed of peat soil and bark powder uniformly mixed in a mass ratio of 3:1.

Described WPM culture medium, by every liter, its raw material composition is ammonium nitrate 400mg, calcium nitrate 556mg, potassium sulfate 990mg, calcium chloride 96mg, potassium dihydrogen phosphate 170mg, sodium molybdate 0.25mg, magnesium sulfate 370mg, sulfate Manganese 22.4mg, Zinc Sulfate 8.6mg, Copper Sulfate 0.25mg, Ferrous Sulfate 27.8mg, Na ₂ -EDTA 37.3mg, Inositol 100mg, Niacin 0.5mg, Vitamin B6 0.5mg, Glycine 2.0mg, Sucrose 20g, Agar 6g , Zeatin 0.5mg.

The sterile water is cooling water that is boiled at 80° C. for 10 minutes and then cooled to normal temperature.

Example 2

A method for raising blueberry seedlings, comprising the following steps:

(1) Select semi-lignified branches, cut them into 1.5cm single-bud branches, soak them in saturated washing powder water for 11 minutes, then rinse them with tap water for 11 minutes, and then use 70% sodium hypochlorite under aseptic conditions After soaking for 6 minutes, soak in mercuric chloride solution with a mass concentration of 0.1% for 9 minutes, then rinse with sterile water for 6 times, then inoculate it on WPM medium for cultivation, control the temperature at 26°C, and light intensity at 2100LX. The light time is 17h/day;

(2) After step 1) is cultivated until the plant grows to a plant height of 8 cm, it is transferred to the rooting medium for rooting treatment.

Described rooting process is culture rooting in the bottle.

The cultivation rooting in described bottle is that the plant height of 8cm is transferred in the rooting medium, wherein the rooting medium is contained in a wide-mouth bottle, and its rooting medium is 1/2WPM medium adding IBA, so that IBA The concentration in the culture medium is 0.1mg/L, and then it is placed at a temperature of 26°C, a light intensity of 2000K, and a light time of 17h/day, and cultured for two months.

Described rooting medium is culture rooting medium in the bottle, and this medium is 1/2WPM medium and adds IBA, makes the concentration of IBA in the medium be 0.1mg/L.

Described WPM culture medium, by every liter, its raw material composition is ammonium nitrate 400mg, calcium nitrate 556mg, potassium sulfate 990mg, calcium chloride 96mg, potassium dihydrogen phosphate 170mg, sodium molybdate 0.25mg, magnesium sulfate 370mg, sulfate Manganese 22.4mg, Zinc Sulfate 8.6mg, Copper Sulfate 0.25mg, Ferrous Sulfate 27.8mg, Na ₂ -EDTA 37.3mg, Inositol 100mg, Niacin 0.5mg, Vitamin B6 0.5mg, Glycine 2.0mg, Sucrose 20g, Agar 6g , Zeatin 1mg.

The sterile water is tap water boiled at 100° C. for 30 minutes and then cooled to normal temperature.

Example 3

A method for raising blueberry seedlings, comprising the following steps:

(1) Select semi-lignified branches, cut them into 1.3cm single-bud branches, soak them in saturated washing powder water for 10 minutes, rinse them with tap water for 10 minutes, and then use 70% sodium hypochlorite under aseptic conditions After soaking for 5 minutes, soak in mercuric chloride solution with a mass concentration of 0.1% for 8 minutes, rinse repeatedly with sterile water for 5 times, then inoculate it on WPM medium for cultivation, control the temperature at 25°C, and light intensity at 2000LX. The light time is 16h/day;

(2) After step 1) is cultivated until the plant grows to a plant height of 7 cm, it is transferred to the rooting medium for rooting treatment.

The rooting treatment is rooting culture of cuttings outside the bottle.

The rooting culture of cuttings outside the bottle is to cut the plants with a plant height of 7cm into 4cm cutting strips, and cut the cutting strips on the rooting medium, the cutting depth is 1.3cm, and before cutting, the mass volume concentration is Soak with 0.1mg/L IBA, and after cuttings, control the temperature at 25°C, light intensity at 1500K, light time at 16h/day, and cultivate for two months.

The rooting medium is a rooting medium for cuttings outside a bottle, which is composed of peat soil and bark powder uniformly mixed in a mass ratio of 3:1.

Described WPM culture medium, by every liter, its raw material composition is ammonium nitrate 400mg, calcium nitrate 556mg, potassium sulfate 990mg, calcium chloride 96mg, potassium dihydrogen phosphate 170mg, sodium molybdate 0.25mg, magnesium sulfate 370mg, sulfate Manganese 22.4mg, Zinc Sulfate 8.6mg, Copper Sulfate 0.25mg, Ferrous Sulfate 27.8mg, Na ₂ -EDTA 37.3mg, Inositol 100mg, Niacin 0.5mg, Vitamin B6 0.5mg, Glycine 2.0mg, Sucrose 20g, Agar 6g , Zeatin 0.8mg.

The sterile water is tap water boiled at 90°C for 20 minutes and then cooled to normal temperature.

Example 4

A method for raising blueberry seedlings, comprising the following steps:

(1) Select semi-lignified branches, cut them into 1.2cm single-bud branches, soak them in saturated washing powder water for 11 minutes, then rinse them with tap water for 9 minutes, and then use 70% sodium hypochlorite under aseptic conditions After soaking for 6 minutes, soak in mercuric chloride solution with a mass concentration of 0.1% for 7 minutes, then rinse with sterile water for 5 times, and then inoculate it on WPM medium for cultivation. The temperature is controlled at 24°C, and the light intensity is 1900LX. The light time is 17h/day;

(2) After step 1) is cultivated until the plant grows to a plant height of 7 cm, it is transferred to the rooting medium for rooting treatment.

Described rooting process is culture rooting in the bottle.

The cultivation rooting in described bottle is that the plant height of 7cm is transferred in the rooting medium, wherein the rooting medium is contained in a wide-mouth bottle, and its rooting medium is 1/2WPM medium adding IBA, so that IBA The concentration in the culture medium is 0.1mg/L, and then it is placed at a temperature of 25°C, a light intensity of 1500K, and a light time of 16h/day, and cultured for two months.

Described rooting medium is culture rooting medium in the bottle, and this medium is 1/2WPM medium and adds IBA, makes the concentration of IBA in the medium be 0.1mg/L.

Described WPM culture medium, by every liter, its raw material composition is ammonium nitrate 400mg, calcium nitrate 556mg, potassium sulfate 990mg, calcium chloride 96mg, potassium dihydrogen phosphate 170mg, sodium molybdate 0.25mg, magnesium sulfate 370mg, sulfate Manganese 22.4mg, Zinc Sulfate 8.6mg, Copper Sulfate 0.25mg, Ferrous Sulfate 27.8mg, Na ₂ -EDTA 37.3mg, Inositol 100mg, Niacin 0.5mg, Vitamin B6 0.5mg, Glycine 2.0mg, Sucrose 20g, Agar 6g , Zeatin 0.9mg.

Described sterile water is distilled water.

Claims (3)

1. a kind of blueberry method for culturing seedlings, which is characterized in that comprise the following steps：

(1) semi-lignified branch is selected, the simple bud branch section of 1-1.5cm is cut into, 9- is impregnated using saturation washing powder water 11min, then 9-11min is rinsed with tap water, then aseptically, mass concentration is used to impregnate 4- for 70% sodium hypochlorite After 6min, then with the mercuric chloride solution that mass concentration is 0.1% impregnate 7-9min, then rinsed 5-6 times repeatedly with sterile water, then by its It is seeded on WPM culture mediums and cultivates, controlled at 24-26 DEG C, intensity of illumination 1900-2100LX, light application time 15- 17h/ days；

(2) cultivate and grown to plant to plant height for after 6-8cm after step 1), and place of taking root is carried out in root media of being transferred Reason, you can；

The WPM culture mediums, in terms of every liter, material composition for ammonium nitrate 400mg, calcium nitrate 556mg, potassium sulfate 990mg, Calcium chloride 96mg, potassium dihydrogen phosphate 170mg, sodium molybdate 0.25mg, magnesium sulfate 370mg, manganese sulfate 22.4mg, zinc sulfate 8.6mg, Copper sulphate 0.25mg, ferrous sulfate 27.8mg, Na₂- EDTA37.3mg, inositol 100mg, niacin 0.5mg, vitamin B₆ 0.5mg、 Glycine 2.0mg, sucrose 20g, agar 6g, 0.5~1mg of zeatin；The rooting treatment for the outer cuttage root-taking culture of bottle or Culture in glassware is taken root；

The outer cuttage root-taking culture of the bottle is that the plant that plant height is 6-8cm is cut into the cuttage item of 3-5cm, and by cuttage item On root media, cuttage depth is 1-1.5cm, and IBA of the mass-volume concentration for 0.1mg/L is used before cuttage for cuttage Dipping, and after cuttage, controlled at 24-26 DEG C, intensity of illumination 1000-2000LX, light application time are 15-17h/ days, training It supports two months, you can；

It is that the plant that plant height is 6-8cm is transferred in root media that the culture in glassware, which is taken root, wherein root media It is to be contained in wide-mouth bottle, root media adds IBA for 1/2WPM culture mediums so that the concentration of IBA in the medium is 0.1mg/L, then place it in that temperature is 24-26 DEG C, intensity of illumination 1000-2000LX, light application time are 15-17h/ days, training It supports two months, you can.

2. blueberry method for culturing seedlings as described in claim 1, which is characterized in that the root media is the outer cuttage root-taking of bottle Culture medium, consisting of peat soil and bark powder are 3 according to mass ratio:1 carries out uniformly mixed mixture.

3. blueberry method for culturing seedlings as described in claim 1, which is characterized in that the sterile water is distilled water or tap water quilt 80-100 DEG C is boiled 10-30min postcoolings to the cooling water of room temperature.