CN105125808A - Ant preparation prepared by microbial fermentation and preparation method of ant preparation - Google Patents
Ant preparation prepared by microbial fermentation and preparation method of ant preparation Download PDFInfo
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- CN105125808A CN105125808A CN201510444224.6A CN201510444224A CN105125808A CN 105125808 A CN105125808 A CN 105125808A CN 201510444224 A CN201510444224 A CN 201510444224A CN 105125808 A CN105125808 A CN 105125808A
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention belongs to the field of biological health preparations and discloses an ant preparation prepared by microbial fermentation and a preparation method of the ant preparation. By application of microbial fermentation engineering theories, engineering beneficial bacterial liquid of bifidobacterium bifidum, candida utilis and lactobacillus reuteri is used for biopharmaceutical production of the ant preparation. Firstly, biological active elements of ants can be extracted more thoroughly; secondly, compatibility of components of traditional Chinese medicines in different assistance functions can be realized according to differences of diseases; thirdly, medicines prepared according to the method is capable of improving curative effects without atopic effects; fourthly, the biological active elements, which are effective active ingredients of ants, can function in synergic disease treatment and health protection with the bifidobacterium bifidum, the candida utilis and the lactobacillus reuteri.
Description
Technical field
The present invention relates to biological health formulation art, particularly a kind of ant preparation utilizing fermentable to prepare and preparation method thereof.
Background technology
Formica fusca belongs to Arthropoda, Insecta, Hymenoptera, Formicidae, maximum in its most species of insecticide circle, existence amount.Global Formica fusca about has 260 genus, kind more than 16000, about has kind more than 2000 in China.Formica fusca is as medicinal, very ancient in China's origin." JINGANG WAN " of Han dynasty treatment muscles and bones weakness, add honey refining ball with Formica fusca pulverizing exactly and form, supplementary Amplifications of the Compendium of Materia Medica claims ant ovum to be ZHUANGYUANZI, nature and flavor are sweet flat, energy physical strength profiting, damp color (beauty treatment), lactogenic juice, for insufficiency of vital energy and blood after being ill, puerperal hypogalactia etc.
World many countries has the example of ant repellent equally.The American Indian in Guyana, South America, utilizes Formica fusca to do the stitching of surgical site infections; Mexico utilizes the secretions of Formica fusca to treat poisonous insect and bites; Suppurative dermatosis, neurodermatitis, always rotten lower limb (chronic ulcer of the lower limb) are treated with Formica fusca by the former Soviet Union; Midland, MI university treatment rheumatic arthritis; Biological family of Australia utilizes Formica fusca to learn treatment fungal infection; Japan with Formica fusca treating chilblain, worm bite, neuralgia; South America Formica fusca treating rheumatic arthritis and stupor; Apoplexy, dizzy, rheumatism, paralysis are controlled with Formica fusca in Europe; Formica fusca treating rheumatic arthritis is used in Africa; Brazil controls epilepsy with Formica fusca; Arabic control sexual impotence and anus repeatly with Formica fusca; Britain's Formica fusca extract is used for painless delivery; India controls wound and resisting fatigue with Formica fusca; Mali Lai Nadao, bar come island, Thailand is short sexy with Formica fusca; Malaysia controls scabies etc. with Formica fusca.
The nutrition of the Chinese Technology Association to polyrhachis measures: its body internal protein content is up to 67%, there are 28 kinds of free amino acids, wherein have 8 kinds by human body necessary, there are the vitamin such as B1, B2, B12 and E, containing 20 various trace elements such as calcium, ferrum, magnesium, iodine, zinc, wherein the abundantest with the content of zinc, also containing multiple enzyme, steroid, triterpenoid, citral, formic acid etc.Ant preparation has significant curative effect for diseases such as deficient property disease, neurosis, rheumatic arthritis, rheumatoid arthritis, sciatica, gout, dermatomyositis, hemiplegia, pulmonary tuberculosis, hepatitis B, chronic cough and asthma, gastropathy, cholecystitis, kinds of tumors, lupus erythematosus, hemorrhoid, post disease alopecia, teen-ager leukotrichia, sexual impotence, seminal emission, sterility and infertility, menoxenia, woman's hyposexuality, puerperal hypogalactia, venom, furunculosis swell and ache.
Chinese medicine is thought, kidney essense weakness is the major reason of human senility, the outstanding role of Formica fusca is kidney invigorating and YANG supporting, the weight of testis and ovary can be made to increase, in convoluted seminiferous tubule, in Interstitial cell and each spermatocyte, the content of DNA, RNA increases, greatly promote cell division, thus can strengthen sexual function of human body.Formica fusca can increase the synthesis of DNA, RNA in immunocyte simultaneously, and immunocyte is increased, and is desirable anatrophic.Effectively can also promote hypertrophy and the growth of the organs such as thymus spleen, be a kind of broad immune synergist.What attract people's attention most is, Formica fusca presents unique two-way immunoregulation effect to cellular immunization, and low immunologic function can be made to improve, and hyperfunction immunologic function is adjusted, reach human body from steady poised state, thus to mankind's building body have that when the water comes, a channel is formed reason.
In sum, the medical value of Formica fusca is very high.But in application aspect, owing to being subject to the restriction of extracting method and processing technique, limit production and the application of ant preparation.By the 420 plurality of Chinese periodicals of nearly 20 years after consulting nineteen ninety-five, up to now in extracting method and processing technique, sum up and roughly have following methods and processing dosage form: 1, decocting: decoction; 2, pulverizing: powder, powder, electuary, capsule, pill; 3, steep in wine: preserved material, tincture; 4, water mill paste, cream.Chinese medicine manufacturing enterprise extraction process roughly has three kinds: 1, water extraction 2, alcohol extracting method 3, salt lifting manipulation.In the existing using method of Formica fusca, the absorbance of its biological activity element provides low.The further utilization modern technologies of science, make full use of the medicinal usefulness of Formica fusca, and the preparation method of research and development ant preparation is most important.
Summary of the invention
In order to the health care of the medical value and collaborative prescription Chinese medicine that embody Formica fusca more fully, ant preparation utilizing fermentable to prepare that the biological activity element that the invention provides a kind of Formica fusca can be fully absorbed and preparation method thereof.
Technical scheme of the present invention is:
Utilizing an ant preparation prepared by fermentable, making through fermenting after being mixed with prescription Chinese medicine by biofermentation Formica fusca engineering bacteria; The bacterial concentration of described biofermentation Formica fusca engineering bacteria is 1-1000cfu/mL; The bacterium liquid of described biofermentation Formica fusca engineering bacteria is by bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri Anaerobic culturel and obtaining in the aqueous solution of Sal, glucose and the mixture of Guangxi Formica fusca powder; Described Candida utilis can tolerate 36-38 DEG C of environment through the domestication cultivation of inoculation, cultivation, purification, rejuvenation; The concentration of described Sal is 2-18g/L, and the concentration of glucose is 3-40g/L, and the addition of Guangxi Formica fusca powder is 5-100g/L.
Preferably, the bacterial concentration of described biofermentation Formica fusca engineering bacteria is 80-120cfu/mL; The concentration of Sal is 12g/L, and the concentration of glucose is 18g/L, and the addition of Guangxi Formica fusca powder is 30-60g/L.
The preparation method of the described ant preparation utilizing fermentable to prepare, concrete steps are:
1) Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, can tolerate 36-38 DEG C environment and under the environment of 36-38 DEG C well-grown;
2) prepare the mixed aqueous solution of Sal and glucose, wherein, the concentration of Sal is 2-18g/L, and the concentration of glucose is 3-40g/L; In described mixed aqueous solution, add Guangxi Formica fusca powder, obtain Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder; The addition of Guangxi Formica fusca powder is 5-100g/L;
3) by bifidobacterium bifidum, Lactobacillus reuteri and step 1) gained Candida utilis is inoculated in step 2) gained Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder, Anaerobic culturel 12-48h, obtains biofermentation Formica fusca engineering bacteria bacterium liquid;
4) prescription Chinese medicine is pulverized, and adds step 3) gained biofermentation Formica fusca engineering bacteria bacterium liquid, anaerobic fermentation 12-48h at 36-38 DEG C;
5) ferment complete, filtering filtering residue, obtains Formica fusca liquor preparation.
The preparation method of the described ant preparation utilizing fermentable to prepare, by step 5) gained Formica fusca liquor preparation concentrates, and then spraying dry prepares Formica fusca powder agent formulation.
The preparation method of the described ant preparation utilizing fermentable to prepare, step 1) in, the culture medium of described Candida utilis is 5 ° of B é beerwort 1.0L, agar 15.0g, natural pH; Originally the cultivation temperature of described Candida utilis is 28-30 DEG C; With the method that each inoculated and cultured raises 0.5 DEG C, Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, until Candida utilis can tolerate the temperature of 36-38 DEG C.
The preparation method of the described ant preparation utilizing fermentable to prepare, step 2) in the concentration of Sal be 12g/L, the concentration of glucose is 18g/L, and the addition of Guangxi Formica fusca powder is 30-60g/L; Guangxi Formica fusca powder is crushed to 40-60 order.
The preparation method of the described ant preparation utilizing fermentable to prepare, step 3) in, the concentration of gained biofermentation Formica fusca engineering bacteria bacterium liquid is 80-120cfu/mL.
The preparation method of the described ant preparation utilizing fermentable to prepare, step 4) in, Chinese medicine powder is broken to 20-60 order.Pulverized by Chinese medicine, be beneficial to raising fermentation efficiency, being crushed to 40-80 order does not affect ferment effect, and later stage easy filtration treatment.
Further, step 5) in, first adopt the plate frame filter filter cleaner being provided with 100 mesh filter screens, then adopt 120-250 mesh sieve again to filter.First coarse filtration, then essence is filtered, the rate of filtration is fast, and good filtration effect.
The preparation method of the described ant preparation utilizing fermentable to prepare, it is 350kg/h that Formica fusca liquor preparation is placed in evaporability, and vacuum is concentrated in the concentration tank of-0.09Mpa.
Ministry of Public Health announces integration of edible and medicinal herbs article, can be used for, in health food article list, having expressly provided the fungus strain list that can be used for health food: Candida utilis (Candidautilis) comes the first two position; Ministry of Health of the People's Republic of China's bulletin (2003 No. 3), according to " prebiotic mushroom health food evaluation regulation " (defend method prison and send out [2001] No. 84 adnexaes 4), have approved Lactobacillus reuteri (Lactobacillusreuteri) for can be used for the probiotic bacteria strain of health food; Bifidobacterium bifidum (Bifidobacteriumbifidum) is for can be used for the probiotic bacteria strain of health food.
Two qi bacillus bifiduss are the healthy required dominant microfloras of human intestines and stomach, along with people from birth to death.The gastroenteropathy Main Pathogenic Bacteria such as helicobacter pylori and escherichia coli in gastrointestinal tract can be killed fast, to duodenal ulcer, gastric ulcer, chronic gastritis, chronic enteritis, colitis Be very effective; Lactobacillus reuteri is the lactobacillus that almost can be present in all vertebratess and mammalian gut reported at present, is the beneficial bacteria of intestinal tract with beneficial function; Candida utilis then can be degraded the toxin of Formica fusca and Chinese medicine or pesticide residues in fermentation.
Described prescription Chinese medicine is the Chinese medicine of the common prescription Chinese medicine for the treatment of various disease in daily life, the prescriptions such as such as auxiliary treatment immune system, auxiliary treatment skeletal system, auxiliary treatment reproductive system, auxiliary treatment musculature, auxiliary treatment nervous system, auxiliary treatment blood circulation, auxiliary treatment respiratory system, auxiliary treatment urinary system, auxiliary treatment integumentary system, auxiliary treatment hormonal system, auxiliary treatment digestive system.
The Chinese medicine formula of auxiliary enhancing immunity:
Hypoimmunity: Radix Aconiti Lateralis Preparata 20g, Rhizoma Zingiberis 30g, Radix Glycyrrhizae Preparata 40g; Susceptible wind and cold adds Ramulus Cinnamomi 15g, Radix Paeoniae Alba 15g; Weak obvious person adds Radix Astragali 30g; Deficiency of YIN xerostomia person adds 15g Radix Ophiopogonis, Radix Rehmanniae 15g.
The Chinese medicine formula of auxiliary treatment skeletal system:
The each option share head necrosis of insufficiency of kidney-YANG
Radix Angelicae Sinensis 12g, raw Radix Rehmanniae Preparata 9g, Radix Paeoniae Rubra 9g, Rhizoma Chuanxiong 9g, Fructus Corni 12g, Radix Aconiti Lateralis Preparata 6g, Cortex Cinnamomi 6g, Fructus Psoraleae 9g, hair Rhizoma Zingiberis Recens 9g, Rhizoma Dioscoreae 12g, Herba Epimedii 9g, Rhizoma Curculiginis 4g, Radix Angelicae Pubescentis 12g.
Bushen Tongluo, soreness of the waist and knees, joint pain: each 15g of Flos Carthami, Radix Paeoniae Rubra, Fructus Jujubae.
The Chinese medicine formula of auxiliary treatment reproductive system:
Foot is fainted and cloudy to be hindered by cold-evil, atrophy of penis person:
Fructus Corni 10g, Fructus Evodiae 10g, Flos Iridis Chinensis 15g, Fructus Foeniculi 8g, Pericarpium Citri Reticulatae Viride 12g, Rhizoma Dioscoreae 15g, Cortex Cinnamomi 10g.
Women's the kidney invigorating: Radix Angelicae Sinensis 30g, Herba Leonuri 50g.
The Chinese medicine formula of auxiliary treatment musculature:
Amyotrophy muscle force recovering: Radix Codonopsis 20g, Cao Shi 25g, snow river 15g, Semen Coicis 15g, wooden main 10g, Fructus Aurantii 10g, Bombyx Batryticatus 10g, mountain cornel 10g, Semen Cuscutae 10g, Rhizoma Dioscoreae 12g, Fructus Lycii 12g, Pericarpium Citri Reticulatae 8g, Rhizoma Cimicifugae 8g, Radix Glycyrrhizae Preparata 6g, Placenta Hominis 10g, Herba Ephedrae 1g, very little sub-10g, Cordyceps 3g, Squama Manis 10g.
The neural Chinese medicine formula of auxiliary treatment:
Type of deficiency of YIN leading to hyper activity of YANG neurasthenia: Radix Rehmanniae Preparata 15g, Fructus Corni 15g, Rhizoma Dioscoreae 12g, Cortex Moutan 12g, Rhizoma Alismatis 12g, Poria 15g, Fructus Lycii 15g, Flos Chrysanthemi 6g, Semen Ziziphi Spinosae 10g, Arillus Longan 18g.
Deficiency of both the heart and spleen type neurasthenia: each 15g of Radix Salviae Miltiorrhizae, Arillus Longan, Semen Ziziphi Spinosae.
Deficiency of both the heart and spleen type neurasthenia: Fructus Mori, Radix Rehmanniae Preparata, each 15g of the Radix Paeoniae Alba.
Heart kidney yin deficiency type neurasthenia: Bulbus Lilii 10g, Radix Ophiopogonis 15g, Radix Rehmanniae 30g, Cortex Phellodendri 6g, Medulla Junci 1g.
Heart kidney yin deficiency type neurasthenia: Radix Codonopsis 15g, Radix Ophiopogonis 12g, Fructus Schisandrae Chinensis 10g.
Heart kidney yin deficiency type neurasthenia: Semen Ziziphi Spinosae (parched) 15g, Mel 30g.
Kidney-Yang deficiency type neurasthenia: 10 pieces, Semen Castaneae, Arillus Longan 15g.
Neurasthenia and intractable insomnia: Ziziphi Spinosae tree root 50g, Radix Salviae Miltiorrhizae 12g.
The Chinese medicine formula of auxiliary treatment blood circulation:
Viral myocarditis: Radix Codonopsis 25-30g, each 15-20g of Radix Salviae Miltiorrhizae, Radix Paeoniae Rubra, Rohdea japonica Roth, Rhizoma Chuanxiong, Radix Sophorae Flavescentis, each 6-10g of Ramulus Cinnamomi, Fructus Schisandrae Chinensis, Poria, each 10-15g of Herba Plantaginis, Radix Glycyrrhizae 9-12g.If cardiopalmus notably adds Cortex et Radix Polygalae (processed), Semen Platycladi, each 10-15g of Concha Margaritifera; If person uncomfortable in chest adds Cortex Albiziae, each 8-12g of Fructus Citri Sarcodactylis; If with diseases caused by exogenous pathogenic factor pyretic toxicity person, add Fructus Forsythiae, each 25-30g of Flos Lonicerae.
Coronary heart disease: Hirudo, each 3g of Aspongopus, Radix Curcumae 9g, Herba Artemisiae Scopariae 30g.
Hypertension: Flos Lonicerae, each 24-30g of Flos Chrysanthemi.If dizzy obviously person, adds Folium Mori 12g; If arteriosclerosis, blood fat height person add Fructus Crataegi 24-30g.
Hemiparalysis after apoplexy: LUMBRICUS 30g, Scolopendra 1, Radix Angelicae Dahuricae 9g.
Cerebral thrombosis: Semen Persicae 10-15g, each 10-15g of Flos Carthami, Rhizoma Chuanxiong, Radix Angelicae Sinensis 10-30g, Radix Paeoniae Rubra 15g, Squama Manis 10g, Caulis Spatholobi 30g.
The Chinese medicine formula of auxiliary treatment respiratory system:
Treatment repeated cold cough: Herba Schizonepetae 6g, purple first 6g, Radix Stemonae 6g, Radix Platycodonis 6g, Pericarpium Citri Reticulatae 6g, Radix Glycyrrhizae 3g, Radix Scutellariae 6g, Semen Armeniacae Amarum 6g, Flos Farfarae 6g, Fructus Schisandrae Chinensis 3g, your branch 6g, Rhizoma Atractylodis Macrocephalae 6g, Radix Isatidis 10g, thin He 3g, Fructus Jujubae two, two panels Rhizoma Zingiberis Recens.
Cough is more than: Semen Armeniacae Amarum, Radix Adenophorae (Radix Glehniae) for 5g, Pericarpium Pyri 15g, Bulbus Fritillariae Cirrhosae 10g gram, crystal sugar 10g.
Pneumonia: Herba Ephedrae, Semen Armeniacae Amarum, Radix Glycyrrhizae, each 15g of Gypsum Fibrosum.
The Chinese medicine formula of auxiliary treatment urinary system:
Damp-heat syndrome: Herba Plantaginis 60 grams, fresh Herba Houttuyniae 60 grams.
Blood-heat syndrome: 250 milliliters, fresh Rhizoma Nelumbinis juice, Sucus Vitis viniferae 250 milliliters, 200 grams, the Radix Rehmanniae, Mel is appropriate.
Syndrome of deficiency of QI: Radix Astragali 30 grams, Semen oryzae sativae 100 grams, 1 gram, Pericarpium Citri Reticulatae end.
Yin deficiency syndrome: 100 milliliters, fresh Rhizoma Nelumbinis juice, whitish honey 30 milliliters, 60 milliliters, Radix Rehmanniae juice.
Asthenia of both the spleen and kidney: Radix Astragali 30 grams, Rhizoma Imperatae 30 grams, Herba Cistanches 20 grams, Exocarpium Citrulli 60 grams.
The Chinese medicine formula of auxiliary treatment integumentary system:
The diseases such as anxious nettle, pruritus, eczema, pityriasis rosea, psoriasis, contact dermatitis, erythema multiforme belong to wind heat from the beginning of person: each 9g of Flos Lonicerae, Fructus Forsythiae, Herba Schizonepetae, Semen Sojae Preparatum, each 6g of Herba Menthae, Fructus Arctii 9g, Radix Platycodonis 6g, Folium Bambusae 4g, Rhizoma Phragmitis 9g, Radix Glycyrrhizae 5g
The chronic and refractory dermatosiss such as skin pruritus, chronic eczema, psoriasis, palmoplantar pustulosis, urticaria, dermatitis, Behcet disease belong to the heat in blood person of penting up: Radix Scutellariae, Rhizoma Coptidis, Cortex Phellodendri, each 6g of Fructus Gardeniae, each 12g of the Radix Rehmanniae, Radix Angelicae Sinensis, each 9g of Radix Paeoniae Rubra, Rhizoma Chuanxiong.
The card such as drug eruption, contact dermatitis, plant-solar dermatitis, purpura, psoriasis (carrying out the phase) belongs to the outer heap soil or fertilizer over and around the roots person of heat in blood: each 10g of Radix Rehmanniae 30g, Radix Paeoniae Rubra, Cortex Moutan, Gypsum Fibrosum 30g, Rhizoma Anemarrhenae 10g, Flos Lonicerae, each 10g of Fructus Forsythiae, Folium Bambusae 6g, Radix Glycyrrhizae 10g.
The carbuncle and ulcer toxic swellings such as multiple folliculitis, carbuncle, furuncle from the beginning of, pyretic toxicity is coalescent, qi depression to blood stasis person: Flos Lonicerae 15g, Radix Saposhnikoviae, the Radix Angelicae Dahuricae, Radix Angelicae Sinensis, each 9g of Radix Paeoniae Rubra, the each 6g of Olibanum, Myrrha, the each 9g of Bulbus Fritillariae Thunbergii, Radix Trichosanthis, each 9g of Squama Manis 15g, Spina Gleditsiae, Pericarpium Citri Reticulatae, Radix Glycyrrhizae 6g.
The Chinese medicine formula of auxiliary treatment hormonal system:
Goiter: Spica Prunellae 30g, each 20g of Os Draconis, Concha Ostreae, Eupolyphaga Seu Steleophaga, Radix Polygoni Multiflori, each 15g of the Radix Paeoniae Alba, each 8g of Rhizoma Sparganii, Rhizoma Curcumae, Bulbus Fritillariae Thunbergii 10g, Radix Glycyrrhizae 6g.
Hyperthyroidism: each 30g of Radix Pseudostellariae, Concha Ostreae, each 20g of Radix Rehmanniae, the Radix Astragali, Bulbus Fritillariae Thunbergii, Spica Prunellae, Radix Ophiopogonis, Radix Scrophulariae, Fructus Aurantii, each 15g of raw Concha Meretricis Seu Cyclinae.If the eye person of dashing forward, add Rhizoma Acori Graminei 15g; If hyperhidrosis person, increase the weight of Radix Astragali 50-80g, Fructus Tritici Levis 10g; If the hard person of goiter, adds Radix Salviae Miltiorrhizae 30g, Pseudobulbus Cremastrae Seu Pleiones 10g; If weakness of the spleen and stomach person, add Rhizoma Dioscoreae, Semen Lablab Album, Fructus Hordei Germinatus, each 10g of Fructus Setariae Germinatus; If excessiveness of stomach-fire person, add Gypsum Fibrosum 30-50g.
Masculine mastoplasia: Herba Taraxaci 60g, Semen Citri Reticulatae, Semen Litchi, Arillus Longan core, each 15g of Carapax Trionycis (processed), Concha Ostreae 30g, Pericarpium Citri Reticulatae Viride, Squama Manis, Fructus Aurantii, Flos Carthami, Semen Persicae, Radix Paeoniae Rubra, each 10g of Radix Glycyrrhizae.
Female climacteric syndrome: Radix Polygoni Multiflori 15g, RHIIZOMA DIOSCOREAE fr0m Henan of China, Fructus Corni, Rhizoma Curculiginis, Herba Leonuri, the Radix Rehmanniae, each 12g of Radix Rehmanniae Preparata, each 10g of Poria, Cortex Moutan, Radix Angelicae Sinensis (parched), Radix Glycyrrhizae Preparata.
The Chinese medicine formula of auxiliary treatment digestive system
Stomachache: Radix Angelicae Dahuricae 30-60g, Radix Glycyrrhizae 15-30g.If stomach cold type of pain, gain temperature happily by, feed alleviation of pain, pale tongue with white fur, deep-slow pulse, belongs to Deficiency and coldness of spleen and stomach person, adds Ramulus Cinnamomi 9g, Radix Paeoniae Alba 18g; If stomach distending pain, connect and two sides of body, belch is slightly easypro, thin white fur of tongue, stringy pulse, belongs to hyperactive liver-QI attacking the stomach person, adds Rhizoma Cyperi, Radix Bupleuri, each 9g of the Radix Paeoniae Alba; If stomach twinge, becoming severe at night, tongue secretly has blood stasis point, hesitant pulse, belongs to stagnation of blood stasis person, adds Oletum Trogopterori, each 9g of Pollen Tyjphae, Radix Salviae Miltiorrhizae 15g; If hyperchlorhydria person, add Os Sepiae 30g; If irritated, insomniac, adds Concha Margaritifera 30g.
Peptic ulcer: each 15g of Radix Astragali Preparata, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, each 12g of the Radix Paeoniae Alba, Rhizoma Cyperi, Rhizoma Corydalis, each 10g of Radix Glycyrrhizae, each 20g of Radix Salviae Miltiorrhizae, Flos Carthami, Mel 30g.If there is bleeding tendency person, removes Radix Salviae Miltiorrhizae, Flos Carthami, add YUNNAN BAIYAO 0.5g; If vexed, bitter taste, fur yellow patients, add peninjau gardenia 6g; If pantothenic acid severe one, add Os Sepiae 15g.
Taste-blindness rate: Radix Astragali 20g, Radix Angelicae Sinensis, Rhizoma Corydalis, each 9g of Radix Glycyrrhizae, the Radix Paeoniae Alba, each 12g of Pseudobulbus Bletillae (Rhizoma Bletillae), Rhizoma Cyperi 10g, Radix Linderae 7g, Cortex Cinnamomi 3g, Os Sepiae 15g.
Using microbe fermentation engineering of the present invention is theoretical, by the engineering probiotics liquid of bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri, produces for the bio-pharmaceuticals of Formica fusca fermentation preparation.One be can by the biological activity of Formica fusca element extract more thorough; Two is can according to the difference of disease therapy, the Chinese medicine of the different assosting effect of compatibility; Three is the medicines according to said method made, and can reach therapeutic effect more rapidly; Four is that the effective active composition-biological activity element of Formica fusca can play synergism with bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri.
Beneficial effect of the present invention is:
What 1, the biological activity of Formica fusca element can be absorbed is more thorough
Biological activity element in the present invention in fermentation Formica fusca powder can discharge thoroughly, is easy to absorb;
2, can according to the Chinese medicine of the different assosting effect of therapeutic purposes compatibility
Guangxi Formica fusca biological preparation is nontoxic, have no side effect.Therapeutic efficiency is had to the disease of human body different system, simultaneously also can be standby with other drug matching fermentation, reach the object of Synergistic treatment;
3, more dosage form can be prepared
Formica fusca liquor preparation can the preparation such as alcoholic, oral liquid, syrup, liquid soft capsule, and powder can make tablet, capsule; Also injection etc. can be prepared by membrane separation technique;
4, medicine can be quantized more accurately
Because in Formica fusca liquor preparation and powder, biological activity cellulose content is high, absorb thoroughly; The quantification of ant preparation is used and has had reliable foundation;
5, by biofermentation, drug effect is rapider
Prescription Chinese medicine is degraded to the effective ingredient being easy to absorb after fermentation, makes drug effect rapider;
6, bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri and ant preparation can play synergism.In fermentable metabolic process, poisonous and harmful ion in prescription Chinese medicine can be engulfed and degraded by bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri, the effective active be not easily absorbed by the body element macromolecular mass can be degraded to the micromolecule be easily absorbed by the body by the enzyme secreted in metabolism, thus strengthens drug effect.
Detailed description of the invention
Embodiment 1
Utilizing an ant preparation prepared by fermentable, making through fermenting after being mixed with prescription Chinese medicine by biofermentation Formica fusca engineering bacteria; The bacterial concentration of biofermentation Formica fusca engineering bacteria is 120cfu/mL; The bacterium liquid of biofermentation Formica fusca engineering bacteria is by bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri Anaerobic culturel and obtaining in the aqueous solution of Sal, glucose and the mixture of Guangxi Formica fusca powder; Candida utilis can tolerate 37 DEG C of environment through the domestication cultivation of inoculation, cultivation, purification, rejuvenation; The concentration of Sal is 12g/L, and the concentration of glucose is 18g/L, and the addition of Guangxi Formica fusca powder is 50g/L.
The preparation method of the ant preparation utilizing fermentable to prepare, concrete steps are:
1) Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, can tolerate 37 DEG C environment and under the environment of 37 DEG C well-grown; Particularly, the culture medium of Candida utilis is 5 ° of B é beerwort 1.0L, agar 15.0g, natural pH; Originally the cultivation temperature of Candida utilis is 28-30 DEG C; With the method that each inoculated and cultured raises 0.5 DEG C, Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, until Candida utilis can tolerate the temperature of 37 DEG C;
2) prepare the mixed aqueous solution of Sal and glucose, wherein, the concentration of Sal is 12g/L, and the concentration of glucose is 18g/L; In mixed aqueous solution, add Guangxi Formica fusca powder, obtain Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder; The addition of Guangxi Formica fusca powder is 50g/L;
3) by bifidobacterium bifidum, Lactobacillus reuteri and step 1) gained Candida utilis is inoculated in step 2) gained Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder, Anaerobic culturel 36h, obtains biofermentation Formica fusca engineering bacteria bacterium liquid; The concentration of gained biofermentation Formica fusca engineering bacteria bacterium liquid is 120cfu/mL;
4) the neurasthenic prescription Chinese medicine for the treatment of type of deficiency of YIN leading to hyper activity of YANG is chosen: Radix Rehmanniae Preparata 15g, Fructus Corni 15g, Rhizoma Dioscoreae 12g, Cortex Moutan 12g, Rhizoma Alismatis 12g, Poria 15g, Fructus Lycii 15g, Flos Chrysanthemi 6g, Semen Ziziphi Spinosae 10g, Arillus Longan 18g; Prescription Chinese medicine is pulverized 40-80 order, and adds step 3) gained biofermentation Formica fusca engineering bacteria bacterium liquid, anaerobic fermentation 36h at 37 DEG C; The addition of bacterium liquid there was not Chinese medicine for minimum with bacterium liquid;
5) ferment complete, first adopt and be provided with the plate frame filter filter cleaner of 100 mesh filter screens, then adopt 120-250 mesh sieve again to filter, filtering filtering residue, obtains Formica fusca liquor preparation.
Shen, 42 years old, female, suffer from type of deficiency of YIN leading to hyper activity of YANG neurasthenia, have difficulty in going to sleep, the length of one's sleep is few, headache unbearably, irritated; Seek doctor everywhere, all fail to cure.Take Formica fusca liquor preparation prepared by the present embodiment, every day twice; Within 10th, have a headache afterwards and alleviate, more easily fall asleep.Pain without a head after 20 days, without irritated sense, is easy to fall asleep.Follow up a case by regular visits to after half a year, without recurrence.
Embodiment 2
Utilizing an ant preparation prepared by fermentable, making through fermenting after being mixed with prescription Chinese medicine by biofermentation Formica fusca engineering bacteria; The bacterial concentration of biofermentation Formica fusca engineering bacteria is 100cfu/mL; The bacterium liquid of biofermentation Formica fusca engineering bacteria is by bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri Anaerobic culturel and obtaining in the aqueous solution of Sal, glucose and the mixture of Guangxi Formica fusca powder; Candida utilis can tolerate 36 DEG C of environment through the domestication cultivation of inoculation, cultivation, purification, rejuvenation; The concentration of Sal is 15g/L, and the concentration of glucose is 15g/L, and the addition of Guangxi Formica fusca powder is 60g/L.
The preparation method of the ant preparation utilizing fermentable to prepare, concrete steps are:
1) Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, can tolerate 36 DEG C environment and under the environment of 36 DEG C well-grown; Particularly, the culture medium of Candida utilis is 5 ° of B é beerwort 1.0L, agar 15.0g, natural pH; Originally the cultivation temperature of Candida utilis is 28-30 DEG C; With the method inoculating rising 0.5 DEG C at every turn, Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, until Candida utilis can tolerate the temperature of 36 DEG C;
2) prepare the mixed aqueous solution of Sal and glucose, wherein, the concentration of Sal is 15g/L, and the concentration of glucose is 15g/L; In mixed aqueous solution, add Guangxi Formica fusca powder, obtain Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder; The addition of Guangxi Formica fusca powder is 60g/L;
3) by bifidobacterium bifidum, Lactobacillus reuteri and step 1) gained Candida utilis is inoculated in step 2) gained Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder, Anaerobic culturel 30h, obtains biofermentation Formica fusca engineering bacteria bacterium liquid; The concentration of gained biofermentation Formica fusca engineering bacteria bacterium liquid is 100cfu/mL;
4) the prescription Chinese medicine for the treatment of peptic ulcer is chosen: each 15g of Radix Astragali Preparata, Radix Codonopsis, the Rhizoma Atractylodis Macrocephalae, Poria, each 12g of the Radix Paeoniae Alba, Rhizoma Cyperi, Rhizoma Corydalis, each 10g of Radix Glycyrrhizae, each 20g of Radix Salviae Miltiorrhizae, Flos Carthami, Mel 30g; Prescription Chinese medicine is pulverized 40-80 order, and adds step 3) gained biofermentation Formica fusca engineering bacteria bacterium liquid, anaerobic fermentation 30h at 36 DEG C; The addition of bacterium liquid there was not Chinese medicine for minimum with bacterium liquid;
5) ferment complete, first adopt and be provided with the plate frame filter filter cleaner of 100 mesh filter screens, then adopt 120-250 mesh sieve again to filter, filtering filtering residue, obtains Formica fusca liquor preparation;
6) by step 5) gained Formica fusca liquor preparation concentrates, and then spraying dry prepares Formica fusca powder agent formulation; Particularly, it is 350kg/h that Formica fusca liquor preparation is placed in evaporability, and vacuum is concentrated in the concentration tank of-0.09Mpa.
Poplar, man, 28 years old, suffers from peptic ulcer, recurrent exerbation, ulcer place pain; Seek doctor everywhere, all fail to cure completely, easily recur.Take Formica fusca powder agent formulation prepared by the present embodiment, every day twice; Ulcer disappeared completely on 5th.Follow up a case by regular visits to after 1 year, without recurrence.
Embodiment 3
Utilizing an ant preparation prepared by fermentable, making through fermenting after being mixed with prescription Chinese medicine by biofermentation Formica fusca engineering bacteria; The bacterial concentration of biofermentation Formica fusca engineering bacteria is 120cfu/mL; The bacterium liquid of biofermentation Formica fusca engineering bacteria is by bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri Anaerobic culturel and obtaining in the aqueous solution of Sal, glucose and the mixture of Guangxi Formica fusca powder; Candida utilis can tolerate 37 DEG C of environment through the domestication cultivation of inoculation, cultivation, purification, rejuvenation; The concentration of Sal is 8g/L, and the concentration of glucose is 25g/L, and the addition of Guangxi Formica fusca powder is 30g/L.
The preparation method of the ant preparation utilizing fermentable to prepare, concrete steps are:
1) Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, can tolerate 37 DEG C environment and under the environment of 37 DEG C well-grown; Particularly, the culture medium of Candida utilis is 5 ° of B é beerwort 1.0L, agar 15.0g, natural pH; Originally the cultivation temperature of Candida utilis is 28-30 DEG C; With the method that each inoculated and cultured raises 0.5 DEG C, Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, until Candida utilis can tolerate the temperature of 37 DEG C;
2) prepare the mixed aqueous solution of Sal and glucose, wherein, the concentration of Sal is 8g/L, and the concentration of glucose is 25g/L; In mixed aqueous solution, add Guangxi Formica fusca powder, obtain Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder; The addition of Guangxi Formica fusca powder is 30g/L;
3) by bifidobacterium bifidum, Lactobacillus reuteri and step 1) gained Candida utilis is inoculated in step 2) gained Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder, Anaerobic culturel 24h, obtains biofermentation Formica fusca engineering bacteria bacterium liquid; The concentration of gained biofermentation Formica fusca engineering bacteria bacterium liquid is 80cfu/mL;
4) treatment prescription Chinese medicine strumous is chosen: Spica Prunellae 30g, each 20g of Os Draconis, Concha Ostreae, Eupolyphaga Seu Steleophaga, Radix Polygoni Multiflori, each 15g of the Radix Paeoniae Alba, each 8g of Rhizoma Sparganii, Rhizoma Curcumae, Bulbus Fritillariae Thunbergii 10g, Radix Glycyrrhizae 6g; Prescription Chinese medicine is pulverized 50-60 order, and adds step 3) gained biofermentation Formica fusca engineering bacteria bacterium liquid, anaerobic fermentation 24h at 37 DEG C; The addition of bacterium liquid there was not Chinese medicine for minimum with bacterium liquid;
5) ferment complete, filtering filtering residue, obtains Formica fusca liquor preparation.
Lu, 39 years old, man, goiter; Take by above goiter prescription Chinese medicine traditionally decocting method gained traditional Chinese medicine liquid, take continuously and within one month, have not yet to see obvious improvement.Take Formica fusca liquor preparation prepared by the present embodiment, every day twice; 10 obviously take effect, and cure after 20 days.Follow up a case by regular visits to after 1 year, without recurrence.
Claims (10)
1. the ant preparation utilizing fermentable to prepare, is characterized in that: make through fermenting after being mixed with prescription Chinese medicine by biofermentation Formica fusca engineering bacteria; The bacterial concentration of described biofermentation Formica fusca engineering bacteria is 1-1000cfu/mL; The bacterium liquid of described biofermentation Formica fusca engineering bacteria is by bifidobacterium bifidum, Candida utilis, Lactobacillus reuteri Anaerobic culturel and obtaining in the aqueous solution of Sal, glucose and the mixture of Guangxi Formica fusca powder; Described Candida utilis can tolerate 36-38 DEG C of environment through the domestication cultivation of inoculation, cultivation, purification, rejuvenation; The concentration of described Sal is 2-18g/L, and the concentration of glucose is 3-40g/L, and the addition of Guangxi Formica fusca powder is 5-100g/L.
2. the ant preparation utilizing fermentable to prepare as claimed in claim 1, is characterized in that: the bacterial concentration of described biofermentation Formica fusca engineering bacteria is 80-120cfu/mL; The concentration of Sal is 12g/L, and the concentration of glucose is 18g/L, and the addition of Guangxi Formica fusca powder is 30-60g/L.
3. the preparation method of the ant preparation utilizing fermentable to prepare as claimed in claim 1, it is characterized in that, concrete steps are:
1) Candida utilis inoculated, cultivate, purify, the domestication of rejuvenation, can tolerate 36-38 DEG C environment and under the environment of 36-38 DEG C well-grown;
2) prepare the mixed aqueous solution of Sal and glucose, wherein, the concentration of Sal is 2-18g/L, and the concentration of glucose is 3-40g/L; In described mixed aqueous solution, add Guangxi Formica fusca powder, obtain Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder; The addition of Guangxi Formica fusca powder is 5-100g/L;
3) bifidobacterium bifidum, Lactobacillus reuteri and step 1) gained Candida utilis are inoculated in step 2) gained Sal, the aqueous solution of glucose and the mixture of Guangxi Formica fusca powder, Anaerobic culturel 12-48h, obtains biofermentation Formica fusca engineering bacteria bacterium liquid;
4) prescription Chinese medicine is pulverized, and add step 3) gained biofermentation Formica fusca engineering bacteria bacterium liquid, anaerobic fermentation 12-48h at 36-38 DEG C;
5) ferment complete, filtering filtering residue, obtains Formica fusca liquor preparation.
4. the preparation method of the ant preparation utilizing fermentable to prepare as claimed in claim 3, is characterized in that: concentrated by step 5) gained Formica fusca liquor preparation, then spraying dry prepares Formica fusca powder agent formulation.
5. the preparation method of the ant preparation utilizing fermentable to prepare as claimed in claim 3, is characterized in that: in step 1), and the culture medium of described Candida utilis is 5 ° of B é beerwort 1.0L, agar 15.0g, natural pH; Originally the cultivation temperature of described Candida utilis is 28-30 DEG C; With the method that each inoculated and cultured raises 0.5 DEG C, Candida utilis inoculated repeatedly, cultivate, purify, the domestication of rejuvenation, until Candida utilis can tolerate the temperature of 36-38 DEG C.
6. the preparation method of the ant preparation utilizing fermentable to prepare as claimed in claim 3, is characterized in that: step 2) in the concentration of Sal be 12g/L, the concentration of glucose is 18g/L, and the addition of Guangxi Formica fusca powder is 30-60g/L; Guangxi Formica fusca powder is crushed to 40-60 order.
7. the preparation method of the ant preparation utilizing fermentable to prepare as claimed in claim 3, is characterized in that: in step 3), and the concentration of gained biofermentation Formica fusca engineering bacteria bacterium liquid is 80-120cfu/mL.
8. the preparation method of the ant preparation utilizing fermentable to prepare as claimed in claim 3, is characterized in that: in step 4), Chinese medicine powder is broken to 20-60 order.
9. the preparation method of the ant preparation utilizing fermentable to prepare as claimed in claim 8, is characterized in that: in step 5), first adopts the plate frame filter filter cleaner being provided with 100 mesh filter screens, then adopts 120-250 mesh sieve again to filter.
10. the preparation method of the ant preparation utilizing fermentable to prepare as claimed in claim 4, is characterized in that: it is 350kg/h that Formica fusca liquor preparation is placed in evaporability, and vacuum is concentrated in the concentration tank of-0.09Mpa.
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| CN1418943A (en) * | 2002-10-11 | 2003-05-21 | 王凯 | Ant fermentation wine and fermentation process thereof |
| CN1978623A (en) * | 2005-11-30 | 2007-06-13 | 教传勇 | Method for producing ant-moth health wine |
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