CN105116042A - PCR-SSCP (polymerase chain reaction-single strand conformation polymorphism) gel electrophoresis low-temperature operation box - Google Patents

Abstract

The invention discloses a low-temperature operation box for PCR-SSCP gel electrophoresis, which comprises an outer box, a box cover arranged on the top of the outer box and a gel electrophoresis instrument, an inner box is arranged in the outer box, and the side walls of the outer box and the inner box are connected with each other. An ice tank is formed between the bottoms, and ice cubes are filled in the ice tank, and a gel electrophoresis tank is arranged in the inner box, the positive and negative poles of the gel electrophoresis tank are connected to one end of the power cord, and the power cord is another One end passes through the semi-closed hole on the top of the side wall of the outer box and is connected to the positive and negative poles of the gel electrophoresis instrument, and an observation window is arranged in the center of the box cover. In the present invention, the gel electrophoresis tank is placed in the double-layer box composed of the outer box and the inner box, which not only better maintains the low-temperature operating environment of the gel electrophoresis tank, but also forms a gap between the side wall and the bottom of the outer box and the inner box. Ice trough, the ice packs and ice cubes placed in the ice trough are easy to replace or take out, which improves the experimental efficiency.

Description

A low-temperature operation box for PCR-SSCP gel electrophoresis

technical field

The invention relates to a low-temperature operation box for PCR-SSCP gel electrophoresis, belonging to the technical field of gel experiment equipment.

Background technique

Strict PCR-SSCP gel electrophoresis experiments need to be carried out under low temperature conditions. However, there is no special low-temperature operation box for gel electrophoresis. The existing experimental equipment can only perform gel electrophoresis experiments at room temperature. It will affect the accuracy of the experimental results. Some laboratories also use electrophoresis in a 4°C refrigerator to form a low-temperature operating environment. This method has many disadvantages, such as inconvenient operation due to the large size of the refrigerator; high price of the refrigerator, high power consumption, and difficulty in observation.

Contents of the invention

The object of the present invention is to provide a PCR-SSCP gel electrophoresis low-temperature operation box with simple structure, convenient use, low price, easy to take and easy to observe, and the device can provide a low-temperature operation box for PCR-SSCP gel electrophoresis experiments. Operating environment, thereby improving the accuracy of experimental results.

The technical scheme of the present invention: a PCR-SSCP gel electrophoresis low-temperature operation box, comprising an outer box, a box cover arranged on the top of the outer box and a gel electrophoresis instrument, an inner box is arranged in the outer box, and the side of the outer box and the inner box An ice trough is formed between the wall and the bottom, and ice cubes are filled in the ice trough, and a gel electrophoresis tank is arranged in the inner box, the positive and negative poles of the gel electrophoresis tank are connected to one end of the power cord, and the power supply The other end of the line passes through the semi-closed hole on the top of the side wall of the outer box and is connected with the positive and negative poles of the gel electrophoresis instrument, and an observation window is arranged in the center of the box cover.

The outer box is placed in the water tank, and the bottom of the outer box is provided with drainage holes, which can discharge the ice water melted in the ice tank in time.

The height of the inner box is 5-10 cm lower than that of the outer box.

The semi-closed hole is a U-shaped hole, therefore, when the gel electrophoresis tank in the inner box is connected with the gel electrophoresis instrument, the whole inner box can be taken out from the outer box together with the gel electrophoresis instrument, so that Replace the ice pack at the bottom of the gel electrophoresis tank or add ice cubes without affecting the entire experiment.

The outer box, the inner box and the box cover are all made of plastic foam to improve the heat preservation effect of the whole box body.

Due to the adoption of the above technical scheme, the present invention has the advantages that: the present invention places the gel electrophoresis tank in a double-layer box composed of an outer box and an inner box, which not only better maintains the low temperature operating environment of the gel electrophoresis tank, but also keeps the gel electrophoresis tank in the outer box. An ice trough is formed between the side wall and the bottom of the inner box, and the ice packs and ice cubes placed in the ice trough are easy to replace or take out, which improves the experimental efficiency. At the same time, the present invention puts the outer box in the water tank, and a drainage hole is provided at the bottom of the outer box to discharge the ice water melted in the ice tank during the gel electrophoresis experiment, so as to achieve the effect of timely drainage and avoid affecting the tank. body temperature. In addition, the present invention is designed with a transparent observation window on the box cover, which is convenient for observing the electrophoresis condition of the electrophoresis tank placed in the box. Therefore, the device of the present invention not only brings great convenience to the operator, but also overcomes the problems of the existing experimental environment and the like, and can be popularized and used in schools, enterprises, research institutes and other areas with obvious benefits.

Description of drawings

Fig. 1 is the perspective view of structural representation of the present invention;

Fig. 2 is a top view of the structure schematic diagram when the box cover of the present invention is opened.

Explanation of reference signs: 1-outer box, 2-inner box, 3-ice storage tank, 4-box cover, 5-semi-closed hole, 6-observation window, 7-drainage hole, 8-water storage tank, 9-condensation Gel electrophoresis tank, 10-power cord, 11-gel electrophoresis instrument.

Detailed ways

In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

Embodiment of the present invention: the structural representation of PCR-SSCP gel electrophoresis low-temperature operation box is as shown in Figure 1 and Figure 2, comprises outer case 1, is arranged on the case cover 4 and gel electrophoresis instrument 11 of outer case 1 top, described The outer case 1 is placed in the water tank 8, and the bottom of the outer case 1 is provided with a drainage hole 7. An inner box 2 is arranged in the outer box 1, and the height of the inner box 2 is 5 cm lower than that of the outer box 1. An ice groove 3 is formed between the side walls and the bottom of the outer box 1 and the inner box 2. 3 is filled with ice cubes, and a gel electrophoresis tank 9 is arranged in the inner box 2. The positive and negative poles of the gel electrophoresis tank 9 are connected to one end of the power cord 10, and the other end of the power cord 10 is connected to the outer box 1 side. The U-shaped hole 5 at the top of the wall passes through and is connected with the positive and negative poles of the gel electrophoresis instrument 11 , and an observation window 6 is arranged in the center of the case cover 4 . The outer case 1, the inner case 2 and the case cover 4 are all made of plastic foam.

When in use, first spread a layer of ice packs on the bottom of the outer box 1 and fill the gap between the ice packs with crushed ice cubes, then put the inner box 2 into the outer box 1, and at this time form the space between the inner box 2 and the outer box 1. Put ice pack and ice cubes in the ice tank 3 of the same. Next, put the gel electrophoresis tank 9 into the inner box 2 smoothly, connect the positive and negative poles of the gel electrophoresis tank 9 to one end of the power cord 10, and then connect the other end of the power cord 10 from the U-shaped The hole 5 is connected with the positive and negative poles of the gel electrophoresis instrument 11 after passing through. Next, put the prepared experimental sample into the gel electrophoresis tank 9, cover the box cover 4, and finally turn on the switch of the gel electrophoresis instrument 11, and the whole electrophoresis experiment starts. During the whole electrophoresis process, the experiment progress in the box can be observed through the observation window 6 on the box cover 4. If the room temperature is too high and the melting speed of the ice is accelerated, some ice packs in the ice tank 3 can be replaced without affecting the whole gel. Conducting gel electrophoresis experiments.

Claims (5)

1. A low-temperature operation box for PCR-SSCP gel electrophoresis, comprising an outer box (1), a case cover (4) arranged on the top of the outer box (1) and a gel electrophoresis instrument (11), characterized in that: in the outer box ( 1) An inner box (2) is arranged inside, and an ice storage tank (3) is formed between the side wall and the bottom of the outer box (1) and the inner box (2), and the ice storage tank (3) is filled with ice cubes, A gel electrophoresis tank (9) is provided in the inner box (2), the positive and negative poles of the gel electrophoresis tank (9) are connected to one end of the power cord (10), and the other end of the power cord (10) is connected from the outside The semi-closed hole (5) on the top of the side wall of the box (1) is connected with the positive and negative poles of the gel electrophoresis instrument (11) after passing through, and an observation window (6) is provided in the center of the box cover (4). 2. The low-temperature operation box for PCR-SSCP gel electrophoresis according to claim 1, characterized in that: the outer box (1) is placed in the water tank (8), and the bottom of the outer box (1) is provided with drainage hole (7). 3. The low-temperature operation box for PCR-SSCP gel electrophoresis according to claim 1, characterized in that: the height of the inner box (2) is 5-10 cm lower than that of the outer box (1). 4. The low-temperature operation box for PCR-SSCP gel electrophoresis according to claim 1, characterized in that: the semi-closed hole (5) is a U-shaped hole. 5. The low-temperature operation box for PCR-SSCP gel electrophoresis according to claim 1, characterized in that: the outer box (1), the inner box (2) and the box cover (4) are all made of plastic foam .