CN105062888A - Magnetic rod pipe sleeves, multi-link assembly and nucleic acid extraction instrument - Google Patents
Magnetic rod pipe sleeves, multi-link assembly and nucleic acid extraction instrument Download PDFInfo
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- CN105062888A CN105062888A CN201510556307.4A CN201510556307A CN105062888A CN 105062888 A CN105062888 A CN 105062888A CN 201510556307 A CN201510556307 A CN 201510556307A CN 105062888 A CN105062888 A CN 105062888A
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 52
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 51
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 51
- 230000005291 magnetic effect Effects 0.000 title abstract description 30
- 238000000605 extraction Methods 0.000 title abstract description 13
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 22
- 238000003756 stirring Methods 0.000 claims abstract description 11
- 238000003032 molecular docking Methods 0.000 claims description 20
- 238000009434 installation Methods 0.000 claims description 10
- 230000002209 hydrophobic effect Effects 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 3
- 238000000576 coating method Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
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- 230000005540 biological transmission Effects 0.000 description 8
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- 238000002156 mixing Methods 0.000 description 6
- 229940088623 biologically active substance Drugs 0.000 description 5
- 238000005336 cracking Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
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- 239000002245 particle Substances 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
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- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 230000008450 motivation Effects 0.000 description 2
- 238000001821 nucleic acid purification Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
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- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000003196 chaotropic effect Effects 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/284—Magnetic plugs and dipsticks with associated cleaning means, e.g. retractable non-magnetic sleeve
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/025—High gradient magnetic separators
- B03C1/031—Component parts; Auxiliary operations
- B03C1/033—Component parts; Auxiliary operations characterised by the magnetic circuit
- B03C1/0332—Component parts; Auxiliary operations characterised by the magnetic circuit using permanent magnets
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/02—Magnetic separation acting directly on the substance being separated
- B03C1/28—Magnetic plugs and dipsticks
- B03C1/286—Magnetic plugs and dipsticks disposed at the inner circumference of a recipient, e.g. magnetic drain bolt
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/18—Magnetic separation whereby the particles are suspended in a liquid
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C2201/00—Details of magnetic or electrostatic separation
- B03C2201/26—Details of magnetic or electrostatic separation for use in medical or biological applications
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Abstract
The invention relates to magnetic rod pipe sleeves, a multi-link assembly and a nucleic acid extraction instrument. The nucleic acid extraction instrument comprises the multi-link assembly, a fixing device and a power mechanism, wherein the power mechanism drives the magnetic rod pipe sleeves to rotate and is connected with the fixing device. The magnetic rod pipe sleeves, the multi-link assembly and the nucleic acid extraction instrument have the beneficial effects that the magnetic rod pipe sleeves on the multi-link assembly are driven by the power mechanism to rotate at high speed relative to a support plate; the rotation speeds of the magnetic rod pipe sleeves can be adjusted by adjusting the speed of the power mechanism, so that the requirement of flow of high-viscosity magnetic beads can be met; stirring structures stir reagent liquid at high speed by driving the magnetic rod pipe sleeves to rotate at high speed, thus eliminating the phenomenon of splash of the reagent liquid, widening the uniformly mixed area, fully adsorbing, washing and eluting the magnetic beads, greatly increasing the nucleic acid yield and also reducing the nucleic acid extraction instrument production cost.
Description
Technical field
The present invention relates to nucleic acid extraction technical field, particularly relate to a kind of bar magnet pipe box, multi-joint assembly and instrument for extracting nucleic acid.
Background technology
Extract cell at multiple fields such as modern molecular biology research, clinical disease diagnosis, blood transfusion, food safety detection, and the biologically active substance such as protein, nucleic acid has vital role.Wherein, the biologically active substance compound that nucleic acid is become by many nucleotide polymerization is one of the most base substance of life.Nucleic acid is extensively present in all animals, vegetable cell, microorganism, in organism.Different according to chemical constitution, nucleic acid can be divided into Yeast Nucleic Acid, is called for short RNA and thymus nucleic acid, is called for short DNA.DNA is the essential substance basis storing, copy and convey hereditary information, and RNA plays an important role in protein building-up process.The development of biotechnology is maked rapid progress, along with PCR (PolymeraseChainReaction, polymerase chain reaction) technology is in the application of every field, comprise medical science aspect disease detection, agriculture aspect detection GMOs and other very many-sided application etc., need high-throughout sample biologically active substance to extract and the method for full-automatic biologically active substance extraction and instrument.But in its tangible actual application, often because impurity many in conventional sample and DNA mix, affect, disturb, even suppress DNA, RNA reaction process, make experimental result complicated.
To the pure process of the Isolation and purification of sample cell, DNA, RNA, albumen, so that its product is used for the interpretation of all kinds of downstreams, it is the key link solving round pcr practical application.The classical way of purifying has: boiling lysis, phenol chloroform extraction, silicon fiml absorption method, Beads enrichment method etc.Wherein, Beads enrichment method utilizes and the magnetic bead of specific recognition cell, protein or nucleic acid can extract biologically active substance.Magnetic bead can be combined specifically with nucleic acid molecule, then under the influence of a magnetic field, separates from the active macromolecules blood, animal tissues, food, pathogenic micro-organism equal samples.Beads enrichment method has four primary process links to form: (1) cracking: the DNA in release sample; (2) adsorb: adjust on the specific pellosil being adsorbed onto magnetic bead exoperidium; (3) wash: the magnet that magnetic bead is isolated outside sleeve pipe is fixed, impurity is removed in washing; (4) wash-out: the pellosil of nucleic acid from magnetic bead exoperidium is eluted by changing the condition such as pH value, chaotropic salt.
Beads enrichment method automatic extracting instrument is divided into bar magnet method and suction method.Suction method is undertaken by automatic pipetting device.For paramagnetic particle method nucleic acid purification instrument, add the absorption of cracking process magnetic bead by automatic pipetting device; Take solution away, add rinsing liquid, magnetic bead adsorbs, and takes rinsing liquid away, adds elution buffer.Owing to not taking magnetic bead away time suction method exists and removes waste liquid, liquid-transfering device can not lean on magnetic bead too near, in case magnetic bead together with waste liquid by the problem taken out, affect elution efficiency and purity.
Bar magnet method extraction method is the technology that developed recently gets up, and its principle is that magnetic separating device is immersed liquid, makes the particle that can be attracted magnetically in mixture concentrate on separating device surface by the action of a magnetic field.Wherein in a kind of mode, magnetic separating device is a sleeve pipe, makes sleeve pipe produce magnetic by inserting bar magnet, attract the granular absorption that can be attracted magnetically in mixture to sleeve surface, when bar magnet is detached, sleeve pipe loses magnetism, and the particle be attracted magnetically departs from sleeve pipe.Drive the mechanism of bar magnet that bar magnet can be driven to move up and down in sleeve pipe, contribute to the cracking of leaching process, rinsing and purifying.
But nucleic acid purification instrument in the market all adopts bar magnet TIP to overlap the OQ t of isolation to simulate reagent mixing action, but all can occur mixing insufficient phenomenon, thus cause Beads enrichment to be tested nucleic acid yield is actual cannot close to 100%.
Summary of the invention
Based on this, be necessary, for the low problem of above-mentioned nucleic acid extraction yield, to provide a kind of bar magnet pipe box.
A kind of bar magnet pipe box, comprise sidewall and base plate, described sidewall and base plate surround the cavity for holding bar magnet that has opening, described bar magnet pipe box also comprises the whipped-up structure for stirring reagent solution and the docking structure for docking with the actuating unit of instrument for extracting nucleic acid, described whipped-up structure is located on described sidewall and/or base plate, and described docking structure is located on described sidewall.
Wherein in an embodiment, described whipped-up structure is located on the outside surface of described sidewall and/or base plate.
Wherein in an embodiment, described whipped-up structure comprises the projection be in the form of sheets located on described base plate and/or wall outer surface.
Wherein in an embodiment, one end relative with base plate on described sidewall offers the extension for supporting described bar magnet pipe box.
Wherein in an embodiment, described extension is the protuberance protruding from described wall outer surface.
Wherein in an embodiment, described docking structure comprises the butt hole matched with actuating unit, and described butt hole is opened on described extension.
Wherein in an embodiment, described base plate conically or described base plate be made up of hydrophobic material or described plate outer surface apply hydrophobic coating.
Above-mentioned bar magnet pipe box, high speed rotating is realized to fetching by the actuating unit of docking structure and instrument for extracting nucleic acid, reagent solution is stirred by whipped-up structure while rotation, eliminate the phenomenon that reagent solution splashes, make mixing region wide, fully, nucleic acid yield is greatly improved, and also reduces the production cost to instrument for extracting nucleic acid simultaneously for magnetic bead absorption, washing, wash-out.
For the problem that above-mentioned nucleic acid extraction yield is low, also provide a kind of multi-joint assembly.
A kind of multi-joint assembly, comprise back up pad and several above-mentioned bar magnet pipe boxes, described back up pad is sheathed on bar magnet pipe box described in several, and described bar magnet pipe box can rotate relatively with described back up pad.
Wherein in an embodiment, described back up pad offers the through hole passed for described bar magnet pipe box and the tooth mouth that can pass through for described whipped-up structure, described through hole communicates with described tooth mouth.
Above-mentioned multi-joint assembly, comprises back up pad and bar magnet pipe box, and back up pad is for supporting bar magnet pipe box, and bar magnet pipe box is relative support plate high speed rotating under the drive of actuating unit.Bar magnet pipe box stirs reagent solution by whipped-up structure while rotation, eliminate the phenomenon that reagent solution splashes, make mixing region wide, magnetic bead absorption, washing, wash-out are abundant, nucleic acid yield is greatly improved, and also reduces the production cost to instrument for extracting nucleic acid simultaneously.
For the problem that above-mentioned nucleic acid extraction yield is low, also provide a kind of instrument for extracting nucleic acid.
A kind of actuating unit be fixedly connected with described stationary installation comprising stationary installation, above-mentioned multi-joint assembly and drive described bar magnet pipe box to rotate relative to described back up pad.
Above-mentioned instrument for extracting nucleic acid, the actuating unit be connected with stationary installation comprising multi-joint assembly, stationary installation and drive bar magnet pipe box to rotate, drive bar magnet pipe box on multi-joint assembly relative to back up pad high speed rotating by actuating unit, the speed of rotation of bar magnet pipe box adjusts by the speed of motivation of adjustment mechanism, can meet the requirement of high viscosity magnetic bead flowing; By driving bar magnet pipe box high speed rotating, making whipped-up structure high-speed stirring reagent solution, eliminating the phenomenon that reagent solution splashes, make mixing region wide, fully, nucleic acid yield is greatly improved, and also reduces the production cost to instrument for extracting nucleic acid simultaneously for magnetic bead absorption, washing, wash-out.
Accompanying drawing explanation
Fig. 1 is the structural representation of present pre-ferred embodiments bar magnet pipe box;
Fig. 2 is present pre-ferred embodiments bar magnet sleeve pipe structure diagrammatic top view;
Fig. 3 is present pre-ferred embodiments bar magnet sleeve pipe structure schematic isometric;
Fig. 4 is the supporting plate structure schematic diagram of the multi-joint assembly of present pre-ferred embodiments;
Fig. 5 is the structural representation of the multi-joint assembly of present pre-ferred embodiments;
Fig. 6 is present pre-ferred embodiments instrument for extracting nucleic acid part body schematic diagram.
Embodiment
Please refer to Fig. 1 ~ Fig. 3, present pre-ferred embodiments provides a kind of bar magnet pipe box 100.Bar magnet pipe box 100 comprises sidewall 110 and base plate 120.Sidewall 110 and base plate 120 surround the cavity 111 for holding bar magnet that has opening.Bar magnet pipe box 100 also comprises the whipped-up structure 130 for stirring reagent solution and the docking structure for docking with the actuating unit 310 of instrument for extracting nucleic acid.Whipped-up structure 130 is located on sidewall 110 and/or base plate 120.Docking structure is located on sidewall 110.This bar magnet pipe box 100 coordinates bar magnet, relative with the actuating unit 310 of instrument for extracting nucleic acid by docking structure, thus rotates under the drive of dynamic structure, stirs reagent solution, thus can reach the effect of Beads enrichment by whipped-up structure 130.
Sidewall 110 forms cavity 111 around base plate 120, at cavity 111 opening part, namely relative with base plate 120 on sidewall 110 one end offers extension 150.When using this bar magnet pipe box 100 to carry out Beads enrichment experiment, being matched with back up pad 210 by this extension 150, this bar magnet pipe box 100 can be located, this bar magnet pipe box 100 can normally be worked.Concrete, this extension 150 is for protruding from the protuberance of sidewall 110 outside surface.Like this, when directly being placed through back up pad 210 by bar magnet pipe box 100, can realize the location in back up pad 210, such locator means, structure is simple.This protuberance can be an annulus on sidewall 110 outside surface, and to be namely arranged at sidewall 110 circumferentially whole for protuberance; Protuberance also can in scattered projection on sidewall 110 outside surface.Projection is at least two, and each projection is all positioned on same square section, and the position that such guarantee is located when bar magnet pipe box 100 is positioned in back up pad 210 is stable.
The offer position of docking structure on bar magnet pipe box 100 is not specifically limited, and in the present embodiment, docking structure is located on extension 150.Docking structure comprises the butt hole matched with the actuating unit 310 on instrument for extracting nucleic acid.Namely this butt hole and actuating unit 310 match, and when actuating unit 310 works, by coordinating of actuating unit 310 and butt hole, actuating unit 310 can drive bar magnet pipe box 100 to operate.In other embodiments, actuating unit 310 can also dock by other means with between docking structure, and such as side tooth, fastener etc., be not specifically limited herein, as long as can realize the function driving bar magnet pipe box 100 to rotate.
Whipped-up structure 130 is located on the outside surface of bar magnet pipe box 100 sidewall 110 and/or base plate 120, projection in the form of sheets.When bar magnet pipe box 100 rotates, the whipped-up structure 130 be positioned on sidewall 110 and base plate 120 outside surface can stir reagent solution, and in the process that projection whipped-up structure 130 being set to sheet can be rotated at bar magnet pipe box 100, make stirring convenient, even, similar quant constantly stirs in water.
Base plate 120 is designed to the profile of easy water conservancy diversion, such as taper, curved surface or offer diversion trench, like this, when bar magnet pipe box 100 rotates stopping, the reagent solution remaining in bar magnet pipe box 100 outside surface (outside surface of sidewall 110 and base plate 120) can flow back in Reagent Tube along outside surface.Hydrophobic coating can certainly be applied or base plate 120 is inherently made up of hydrophobic material on the outside surface of base plate 120.Such design can make the reagent solution etc. adhered on base plate 120 to flow back in Reagent Tube smoothly.
Referring to Fig. 4 ~ Fig. 5, a preferred embodiment of the present invention also provides a kind of multi-joint assembly 200.This multi-joint assembly 200 comprises bar magnet pipe box 100 and back up pad 210.Back up pad 210 is sheathed on several identical bar magnet pipe boxes 100, and each bar magnet pipe box 100 can rotate relatively with back up pad 210.Bar magnet pipe box 100 is above-mentioned bar magnet pipe box 100, and its concrete constitutional features does not repeat herein.
Back up pad 210 offers the through hole 211 passed for bar magnet pipe box 100.Because bar magnet pipe box 100 is provided with whipped-up structure 130, smoothly by through hole 211, back up pad 210 can also offer tooth mouth 212 to make whipped-up structure 130.Tooth mouth 212 communicates with through hole 211.Concrete, the number of tooth mouth 212 and concrete offer position, passes through through hole 211 can make bar magnet pipe box 100 and is as the criterion.When bar magnet pipe box 100 passes the through hole 211 in back up pad 210, whipped-up structure 130 is aimed at tooth mouth 212, can ensure that bar magnet pipe box 100 can pass through through hole 211.
The extension 150 that bar magnet pipe box 100 is arranged makes back up pad 210 be sheathed on bar magnet pipe box 100 and there will not be disengaging, namely realizes bar magnet pipe box 100 to be positioned in back up pad 210.
Referring to Fig. 6, a preferred embodiment of the present invention also provides a kind of instrument for extracting nucleic acid.The actuating unit 310 be fixedly connected with stationary installation that this instrument for extracting nucleic acid comprises stationary installation, bar magnet bar 320, multi-joint assembly 200 and drives the bar magnet pipe box 100 on multi-joint assembly 200 to rotate relative to back up pad 210.Multi-joint assembly 200 is above-mentioned multi-joint assembly 200, and the structure of its correspondence repeats no more herein.
Back up pad 210 is fixedly connected in stationary installation.Bar magnet pipe box 100 is sheathed in back up pad 210.Actuating unit 310 is connected with bar magnet pipe box 100, and bar magnet pipe box 100 can be driven to rotate relative to back up pad 210.Bar magnet is placed in described bar magnet pipe box 100, and can move up and down in bar magnet pipe box 100.
Particularly, the type of drive that actuating unit 310 drives bar magnet pipe box 100 to rotate relative to back up pad 210 is not specifically limited.Actuating unit 310 is motor.The motor shaft of motor can be fixedly connected with directly relatively with the docking structure on bar magnet pipe box 100, and like this when machine operation, motor shaft can drive bar magnet pipe box 100 to rotate.Because motor shaft can high speed rotating, bar magnet pipe box 100 also can high speed rotating under the drive of motor shaft.Stir reagent solution by the whipped-up structure 130 on bar magnet pipe box 100 in bar magnet pipe box 100 rotary course, substantially increase nucleic acid yield.
Certainly, in other embodiments, transmission rig 330 can also be increased between motor and bar magnet pipe box 100.Transmission rig 330 comprises the transmission belt be connected with motor shaft and the rotating cylinder be connected with transmission belt.Rotating cylinder is sheathed on outside bar magnet, by motor shaft driven rotary.And the rotation of rotating cylinder does not affect moving up and down of bar magnet bar 320.Rotating cylinder is also connected with docking structure simultaneously, when rotating under the drive of motor shaft, drives bar magnet pipe box 100 to rotate.The type of belt drive of motor to bar magnet pipe box 100 is not specifically limited herein, as long as can transmission be realized.
Owing to being provided with several bar magnet pipe boxes 100 in multi-joint assembly 200, and each bar magnet pipe box 100 all can rotate by relative support plate 210 under the drive of actuating unit 310.Separately can rotate between each bar magnet pipe box 100, also can mutually transmission rotate.When the separate rotation of each bar magnet pipe box 100, each bar magnet pipe box 100 is all connected with actuating unit 310, directly rotates by the drive of actuating unit 310.When between each bar magnet pipe box 100, mutual transmission rotates, one of them bar magnet pipe box 100 can be connected with actuating unit 310, directly rotate by the drive of actuating unit 310, what all the other were remaining is that bar magnet pipe box 100 is connected between two, mutual transmission rotates, and concrete form is not specifically limited herein.
When using instrument for extracting nucleic acid to carry out nucleic acid extraction, first will placed several bar magnet pipe boxes 100 and be fixed in stationary installation.Wherein, the position of number namely in back up pad 210 of concrete bar magnet pipe box 100 is all corresponding with instrument for extracting nucleic acid needing the Reagent Tube extracting nucleic acid.The i.e. all corresponding bar magnet pipe box 100 of each Reagent Tube needing to extract nucleic acid.Start instrument for extracting nucleic acid, bar magnet is moved downward in bar magnet pipe box 100 and stops.Motor starts slow work, in the process of slowly work, docks with the docking structure on bar magnet pipe box 100.After completing mating operation, motor starts rapid operation, to drive bar magnet pipe box 100 high speed rotating.After completing nucleic acid extraction, close instrument for extracting nucleic acid, motor quits work, and bar magnet shifts out bar magnet pipe box 100.
Above-mentioned instrument for extracting nucleic acid, the bar magnet pipe box 100 on multi-joint assembly 200 is driven to rotate relative to back up pad 210 by actuating unit 310, the speed of rotation of bar magnet pipe box 100 adjusts by the speed of motivation of adjustment mechanism 310, can meet the requirement of high viscosity magnetic bead flowing; By the rotation of bar magnet pipe box 100, essentially eliminate the phenomenon of reagent solution splashing, mixing region is wide, and fully, nucleic acid yield is greatly improved, and is tending towards more than 99%, also reduces the production cost to instrument for extracting nucleic acid simultaneously for magnetic bead absorption, washing, wash-out.
Each technical characteristic of the above embodiment can combine arbitrarily, for making description succinct, the all possible combination of each technical characteristic in above-described embodiment is not all described, but, as long as the combination of these technical characteristics does not exist contradiction, be all considered to be the scope that this specification sheets is recorded.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a bar magnet pipe box, it is characterized in that, comprise sidewall and base plate, described sidewall and base plate surround the cavity for holding bar magnet that has opening, described bar magnet pipe box also comprises the whipped-up structure for stirring reagent solution and the docking structure for docking with the actuating unit of instrument for extracting nucleic acid, described whipped-up structure is located on described sidewall and/or base plate, and described docking structure is located on described sidewall.
2. bar magnet pipe box according to claim 1, is characterized in that, described whipped-up structure is located on the outside surface of described sidewall and/or base plate.
3. bar magnet pipe box according to claim 1 and 2, is characterized in that, described whipped-up structure comprises the projection be in the form of sheets located on described base plate and/or wall outer surface.
4. bar magnet pipe box according to claim 1, is characterized in that, one end relative with base plate on described sidewall offers the extension for supporting described bar magnet pipe box.
5. bar magnet pipe box according to claim 4, is characterized in that, described extension is the protuberance protruding from described wall outer surface.
6. bar magnet pipe box according to claim 4, is characterized in that, described docking structure comprises the butt hole matched with actuating unit, and described butt hole is opened on described extension.
7. bar magnet pipe box according to claim 1, is characterized in that, described base plate conically or described base plate be made up of hydrophobic material or described plate outer surface apply hydrophobic coating.
8. a multi-joint assembly, is characterized in that, comprise back up pad and several bar magnet pipe boxes as described in any one of claim 1 ~ 7, described back up pad is sheathed on bar magnet pipe box described in several, and described bar magnet pipe box can rotate relatively with described back up pad.
9. multi-joint assembly according to claim 8, is characterized in that, described back up pad offers the through hole passed for described bar magnet pipe box and the tooth mouth that can pass through for described whipped-up structure, described through hole communicates with described tooth mouth.
10. an instrument for extracting nucleic acid, is characterized in that, the actuating unit be fixedly connected with described stationary installation comprising stationary installation, multi-joint assembly as claimed in claim 8 or 9 and drive described bar magnet pipe box to rotate relative to described back up pad.
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| CN201510556307.4A CN105062888B (en) | 2015-09-02 | 2015-09-02 | Bar magnet pipe sleeve, multi-joint component and instrument for extracting nucleic acid |
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| CN105420074A (en) * | 2016-01-19 | 2016-03-23 | 山西振东基赛生物科技有限公司 | Multifunctional biological substance separating device |
| CN105543090A (en) * | 2016-01-19 | 2016-05-04 | 山西振东基赛生物科技有限公司 | Dehydration device and method |
| CN106906121A (en) * | 2017-04-17 | 2017-06-30 | 新疆出入境检验检疫局检验检疫技术中心 | The nucleic acid separator and separation method of magnet control |
| CN109207340A (en) * | 2017-06-30 | 2019-01-15 | 开启基因股份有限公司 | Fixing tube of nucleic acid extraction assembly and nucleic acid extraction assembly |
| CN110042054A (en) * | 2018-01-16 | 2019-07-23 | 青岛益柏生物科技有限公司 | A kind of automation magnetic bead separating device |
| WO2020037684A1 (en) * | 2018-08-24 | 2020-02-27 | 深圳华大生命科学研究院 | Separation and extraction device for cells and biomolecules, and test system |
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| CN113234591A (en) * | 2021-05-28 | 2021-08-10 | 宁波康程德诺生物医药有限公司 | Integrated nucleic acid rapid-extraction test tube, rapid-extraction detection device and method |
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| CN105420074A (en) * | 2016-01-19 | 2016-03-23 | 山西振东基赛生物科技有限公司 | Multifunctional biological substance separating device |
| CN105543090A (en) * | 2016-01-19 | 2016-05-04 | 山西振东基赛生物科技有限公司 | Dehydration device and method |
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| CN106906121A (en) * | 2017-04-17 | 2017-06-30 | 新疆出入境检验检疫局检验检疫技术中心 | The nucleic acid separator and separation method of magnet control |
| CN109207340A (en) * | 2017-06-30 | 2019-01-15 | 开启基因股份有限公司 | Fixing tube of nucleic acid extraction assembly and nucleic acid extraction assembly |
| CN109207340B (en) * | 2017-06-30 | 2022-08-12 | 开启基因股份有限公司 | Nucleic acid extraction components |
| CN110042054A (en) * | 2018-01-16 | 2019-07-23 | 青岛益柏生物科技有限公司 | A kind of automation magnetic bead separating device |
| WO2020037684A1 (en) * | 2018-08-24 | 2020-02-27 | 深圳华大生命科学研究院 | Separation and extraction device for cells and biomolecules, and test system |
| CN113234591A (en) * | 2021-05-28 | 2021-08-10 | 宁波康程德诺生物医药有限公司 | Integrated nucleic acid rapid-extraction test tube, rapid-extraction detection device and method |
| CN113234591B (en) * | 2021-05-28 | 2024-03-22 | 宁波康程德诺生物医药有限公司 | Integrated nucleic acid quick-lifting test tube, quick-lifting detection device and method |
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