CN105056820B - The micro-structural device that a kind of series connection is amplified - Google Patents

Abstract

The invention belongs to the technical field of microdispersion of multiphase fluids, and in particular relates to a series-amplified microstructure device. The microstructure device is composed of a microchannel substrate, a main channel and a bypass channel. The microchannel substrate is provided with a main channel, one side or both sides of the main channel is provided with a bypass channel, the bypass channel is perpendicular to the main channel; a capillary is embedded in the bypass channel, and the capillary and the main channel form a constriction at the intersection ; The upstream inlet of the main channel is provided with a continuous-phase fluid inlet pipe, and the downstream outlet is provided with a two-phase fluid outlet pipe. The series-amplified microstructure device constituted by the present invention realizes the scale-up of the droplet and bubble breaking process under the jetting flow pattern; the structure is simple, the manufacture is convenient, and it is not easy to be blocked, which greatly improves the generation frequency of monodisperse droplets and bubbles; The invention has good applicability in both gas-liquid and liquid-liquid systems.

Description

A Microstructure Device for Series Amplification

technical field

The invention belongs to the technical field of microdispersion of multiphase fluids, and in particular relates to a series-amplified microstructure device.

Background technique

Monodisperse micro-sized droplets and bubbles are widely used in chemical industries such as food, biological detection, pharmaceutical and cosmetics. In the traditional process, the size distribution of droplets and bubbles obtained by using heterogeneous shear flow is wide, and the energy loss is serious, which is difficult to control and use efficiently. In recent years, microstructured devices with a characteristic size below 1 mm have attracted people's attention. The controllable preparation of monodisperse micro-sized droplets and bubbles can be realized in the microstructured device, which facilitates the regulation of the complex structure of droplets and bubbles. However, at the present stage, microfluidics, especially multiphase fluid microdispersion technology, is often limited to single-stage channels, and the large-scale preparation of monodisperse micro-sized droplets and bubbles faces relatively large technical obstacles, and there is a large gap between them and industrial requirements. gap.

At this stage, the large-scale preparation of micro-sized droplets and bubbles relies on the parallel expansion of microstructure devices, and has achieved preliminary industrial applications. However, the parallel device has high requirements on the manufacturing process, the fluid flow distribution is more complicated, and the operation stability is poor. Both basic research and industrial practice urgently need a more efficient and controllable large-scale preparation method of monodisperse micro-sized droplets and bubbles. .

The present invention proposes for the first time a series amplification structure based on microstructure equipment, and realizes the large-scale preparation of monodisperse liquid droplets, bubbles and particles. The series amplification microstructure device has strong controllability and high stability.

Contents of the invention

The purpose of the present invention is to provide a microstructure device for serial amplification, and the specific technical scheme is as follows:

A series-amplified microstructure device, the microstructure device is composed of a microchannel substrate 1, a main channel 3 and a bypass channel 4;

The microchannel substrate 1 is provided with a series of staggered rectangular channels as the main channel 3, and one or both sides of the main channel 3 are provided with a bypass channel 4, and the bypass channel 4 is perpendicular to the main channel 3; in the bypass channel 4 embedded capillary, the capillary is closely connected with the microchannel substrate 1, and forms a constriction at the junction with the main channel 3, and the constriction is connected in series to form a staggered ladder structure;

The upstream inlet of the main channel 3 is provided with a continuous phase fluid inlet pipe 2, and the continuous phase fluid inlet pipe 2 is sealed and connected with the microchannel substrate 1; the downstream outlet of the main channel 3 is provided with a two-phase fluid outlet pipe 5, and the two-phase fluid outlet pipe 5 It is sealed and connected with the microchannel substrate 1 .

The bypass channel 4 is a series structure that staggers radially up and down or a series structure that staggers radially upwards.

The bypass channels 4 are directly connected to the micro-channel substrate 1 , or the bypass channels 4 are connected to the micro-channel substrate 1 through the upper distribution channel 6 after being collected.

The corner of the main channel 3 is arc or right angle.

The width of the main channel 3 and the bypass channel 4 is 300-1300 μm, and the depth is 300-1300 μm; the radial width of the constriction formed at the intersection of the capillary embedded in the bypass channel 4 and the main channel 3 is 10-100 μm. 300 μm; the pitch of the necking is 500-5000 μm.

The number of series series of the necking series is 2-20.

The inner diameter of the capillary is 50-1000 μm, and the outer diameter is not less than the depth of the bypass channel 4 and the main channel 3 .

The material of the capillary is glass, stainless steel or polytetrafluoroethylene.

The material of the microchannel substrate 1 is polymethyl methacrylate, polydimethylsiloxane, glass, stainless steel, silicon wafer, polytetrafluoroethylene or photosensitive resin.

The beneficial effects of the present invention are: the series-amplified microstructure device described in the present invention realizes the 1-100 μm monodisperse microstructure under the jetting flow pattern through the adjustment of the two-phase phase and the adjustment of the channel structure, based on the shearing effect of the continuous relative dispersed phase. Scale preparation of sized droplets and bubbles. Compared with traditional parallel amplification microstructure equipment, this device has a simple structure, is convenient to manufacture, is not easy to block, and greatly improves the generation frequency of monodisperse droplets and bubbles; it has strong applicability, and can be applied to both gas-liquid and liquid-liquid systems; it is a A low-cost, high-efficiency microstructure amplification device.

Description of drawings

Fig. 1 is one of the structural schematic diagrams of the serially amplified microstructure device of the present invention.

Fig. 2 is an enlarged view of place A (neck place) shown in Fig. 1 .

Fig. 3 is the second schematic diagram of the structure of the serially amplified microstructure device of the present invention.

detailed description

The present invention provides a series-amplified microstructure device. The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

A series-amplified microstructure device. The structure of the microstructure device is shown in Figure 1 and Figure 3, and the above two figures are two typical structural styles of the present invention.

In the device shown in Figure 1, the channels provided on the microchannel substrate have a radially up and down staggered series structure, and Figure 1 shows two different injection modes of the dispersed phase (upper side: injected after the dispersed phase is distributed through the upper distribution channel) main channel; lower side: the dispersed phase is directly injected into the main channel), and both kinds of sampling modes can realize the purpose of the present invention.

Fig. 2 is an enlarged view of A (neck) shown in Fig. 1. In the device shown, the capillary embedded in the bypass channel and the main channel form a constriction structure at the junction.

In the device shown in Figure 3, the channels provided on the microchannel substrate have a radially upward staggered series structure, and Figure 3 indicates the sample injection method in which the dispersed phase is directly injected into the main channel.

The specific meanings of the symbols in the figure are as follows: 1-microchannel substrate, 2-continuous phase fluid inlet pipe, 3-main channel, 4-bypass channel, 5-two-phase fluid outlet pipe, 6-upper distribution channel.

Example 1

Polymethyl methacrylate (PMMA) is used as the material of the microchannel substrate 1. The width and depth of the main channel 3 are both 750 microns, and the width and depth of the bypass channel 4 are both 750 microns. The main channel 3 and the bypass channel 4 are rectangular channels. The radial width of the constriction is 200 microns, and the main channel 3 has ten stages of constriction, and the constriction interval is 1500 microns. A flat quartz capillary with an outer diameter of 750 microns and an inner diameter of 530 microns is embedded in the bypass channel 4 , and the front end is flush with the upper side wall of the upstream main channel 3 . The channel structure is shown in Figure 1. Both sides of the main channel 3 adopt a dispersed phase injection method that is distributed by the upper distribution channel 6 and then injected into the main channel 3 (the structure on the upper side of Figure 1).

The continuous phase was injected using Teflon tubing and the two-phase fluid was collected. Add sodium dodecyl sulfate sodium sulfate (SDS, 0.01wt%-2wt%) or cetyltrimethylammonium bromide (CTAB, 0.01wt%-2wt%) and other aqueous solutions of water-soluble surfactants as a continuous phase, adding different mass concentrations of glycerol (mass fraction in the continuous phase is 1-70wt%) to adjust the viscosity of the continuous phase, the viscosity of the continuous phase is 1mPa·s-500mPa·s. n-hexane is used as the dispersed phase, and the dispersed phase is injected into the upper distribution channel 6 by using a syringe pump, and the dispersed phase fluid is injected into the bypass channel 4 after distribution. Adjusting the two-phase flow can obtain micro-sized monodisperse n-hexane droplet groups with an average size of 1-100 microns and a standard deviation of no more than 5%.

Example 2

Stainless steel is used as the material of the microchannel substrate 1. The width and depth of the main channel 3 are both 300 microns, and the width and depth of the bypass channel 4 are both 300 microns. Both the main channel 3 and the bypass channel 4 are rectangular channels. The radial width of the constriction is 10 microns, and the main channel 3 has ten stages of constriction at intervals of 500 microns. A flat stainless steel capillary with an outer diameter of 300 μm and an inner diameter of 50 μm is embedded in the bypass channel 4 , and the front end of the capillary is flush with the upper side wall of the upstream main channel 3 . The channel structure is shown in FIG. 3 . The upper side of the main channel 3 is injected with the dispersed phase into the main channel 3 by direct injection (the upper side structure in FIG. 3 ).

The continuous phase was injected using Teflon tubing and the two-phase fluid was collected. Add sodium dodecyl sulfate sodium sulfate (SDS, 0.01wt%-2wt%) or cetyltrimethylammonium bromide (CTAB, 0.01wt%-2wt%) and other aqueous solutions of water-soluble surfactants as a continuous phase, adding different mass concentrations of glycerin (the mass fraction in the continuous phase is 1-70wt%) to adjust the viscosity of the continuous phase, the viscosity of the continuous phase is 1mPa·s-500mPa·s. Nitrogen is used as the dispersed phase, and ten strands of the dispersed phase are injected into the continuous phase from the bypass channel 4 by using ten-channel syringe pumps/ten sets of mass flow meters. Adjusting the two-phase flow can obtain micro-scale monodisperse bubble groups with an average size of 5-50 microns and a standard deviation of no more than 5%.

Example 3

Using polytetrafluoroethylene as the material of the microchannel substrate 1, the width and depth of the main channel 3 are both 1300 microns, the width and depth of the bypass channel 4 are both 1300 microns, and both the main channel 3 and the bypass channel 4 are rectangular channels . The radial width of the constriction is 300 microns, and the main channel 3 has ten stages of constriction at intervals of 5000 microns. A stainless steel capillary with an outer diameter of 1300 μm and an inner diameter of 1000 μm is embedded in the bypass channel 4 , and the front end of the capillary is flush with the upper side wall of the upstream main channel 3 . The channel structure is shown in FIG. 3 , and the upper side of the main channel 3 adopts a direct injection method to inject the dispersed phase into the main channel (the upper side structure in FIG. 3 ).

The continuous phase was injected using Teflon tubing and the two-phase fluid was collected. Add sodium dodecyl sulfate sodium sulfate (SDS, 0.01wt%-2wt%) or cetyltrimethylammonium bromide (CTAB, 0.01wt%-2wt%) and other aqueous solutions of water-soluble surfactants as a continuous phase, adding different mass concentrations of polyvinyl alcohol (PVA, the mass fraction in the continuous phase is 1-10wt%) to adjust the viscosity of the continuous phase, the viscosity of the continuous phase is 1mPa·s-500mPa·s. n-octane is used as the dispersed phase, and the dispersed phase is injected into the upper distribution channel 6 by using a syringe pump, and the dispersed phase fluid is injected into the bypass channel 4 after distribution. By adjusting the two-phase flow, micro-sized monodisperse n-octane droplet groups with an average size of 1-100 microns and a standard deviation of no more than 5% can be obtained. Example 4

Using polydimethylsiloxane (PDMS) as the material of the microchannel substrate 1, the width and depth of the main channel 3 are both 810 microns, and the width and depth of the bypass channel 4 are both 810 microns. Channel 4 is a square rectangular channel. The radial width of the constriction is 250 microns, and the main channel 3 has two stages of constrictions with a constriction interval of 1500 microns. A flat stainless steel capillary with an outer diameter of 810 μm and an inner diameter of 510 μm is embedded in the bypass channel 4 , and the front end of the capillary is flush with the upper side wall of the upstream main channel 3 . The channel structure is shown in FIG. 1 , and the upper side of the main channel 3 injects the dispersed phase into the main channel 3 by direct injection (lower structure in FIG. 1 ).

The continuous phase was injected using Teflon tubing and the two-phase fluid was collected. Dodecane/mineral oil with oil-soluble surfactants such as sorbitan oleate (Span80, 0.01wt%-2wt%) or sorbitan trioleate (Span85, 0.01wt%-2wt%) /corn oil solution as the continuous phase, the viscosity of the continuous phase is 1mPa·s～100mPa·s. Nitrogen was used as the dispersed phase, and ten strands of the dispersed phase were injected into the continuous phase from the bypass channel 4 by using a ten-channel syringe pump. Adjusting the two-phase flow can obtain micro-scale monodisperse bubbles with an average size of 5-100 microns and a standard deviation of no more than 3%.

Example 5

Using silicon wafers as the material of the microchannel substrate 1, the width and depth of the main channel 3 are both 750 microns, and the width and depth of the bypass channel 4 are both 750 microns. Both the main channel 3 and the bypass channel 4 are square rectangular channels. The radial width of the constriction is 200 microns, and the main channel 3 has 20 stages of constriction at intervals of 2000 microns. A glass capillary with an outer diameter of 1250 μm and an inner diameter of 1000 μm is embedded in the bypass channel 4 , and the front end of the capillary is flush with the upper side of the upstream main channel 3 . The channel structure is shown in Figure 3. Both sides of the main channel 3 adopt the dispersed phase injection method of injecting into the main channel 3 after being distributed by the upper-level distribution channel 6 (the structure on the upper side of Figure 1).

The continuous phase was injected using Teflon tubing and the two-phase fluid was collected. Dodecane/mineral oil with oil-soluble surfactants such as sorbitan oleate (Span80, 0.01wt%-2wt%) or sorbitan trioleate (Span85, 0.01wt%-2wt%) /corn oil solution as the continuous phase, the viscosity of the continuous phase is 1mPa·s～100mPa·s. Water is used as the dispersed phase, and the dispersed phase is injected into the upper distribution channel 6 by using a syringe pump, and the dispersed phase fluid is injected into the bypass channel 4 after distribution. Adjusting the two-phase flow can obtain micro-scale monodisperse water droplets with an average size of 1-100 microns and a standard deviation of no more than 3%.

Example 6

Using glass as the material of the microchannel substrate 1, the width and depth of the main channel 3 are both 750 microns, and the width and depth of the bypass channel 4 are both 750 microns. Both the main channel 3 and the bypass channel 4 are square rectangular channels. The radial width of the constriction is 200 microns, and the main channel 3 has fifteen stages of constriction, and the constriction interval is 1600 microns. A glass capillary with an outer diameter of 750 μm and an inner diameter of 600 μm is embedded in the bypass channel 4 , and the front end of the capillary is flush with the upper side of the upstream main channel 3 . The channel structure is shown in FIG. 3 , and the upper side of the main channel adopts a direct injection method to inject the dispersed phase into the main channel 3 (the upper side structure in FIG. 3 ).

The continuous phase was injected using a Teflon hose, and the two-phase fluid was collected using an embedded quartz capillary. Add the aqueous solution of water-soluble surfactants such as sodium dodecyl sulfate (SDS, 0.01wt%-2wt%) or cetyltrimethylammonium bromide (CTAB, 0.01wt%-2wt%) as continuous phase, Add different mass concentrations of PVP (polyvinylpyrrolidone, the mass fraction in the continuous phase is 0.1～10wt%) to adjust the continuous phase viscosity, the continuous phase viscosity is 1mPa s～700mPa s, the styrene prepolymer solution (solution composition : Styrene 48wt%-98.9wt%, divinylbenzene 1wt%-50wt%, azobisisobutyronitrile 0.1wt%-2wt%, after heating in 90℃ water bath for 1-5min, styrene prepolymerization can be obtained Body solution) as the dispersed phase, ten strands of the dispersed phase were injected into the continuous phase from the bypass channel 4 by using a ten-channel syringe pump. Adjusting the two-phase flow can obtain micro-scale monodisperse droplets with an average size of 2-100 microns and a standard deviation of no more than 3%. Polystyrene microspheres with an average size of 0.2-50 microns and a standard deviation of no more than 3% are obtained by thermally initiating polymerization of the micro-droplet group, and the microspheres have a complete structure and good stability.

Example 7

Using photosensitive resin as the material of the microchannel substrate 1, the width and depth of the main channel 3 are both 300 microns, and the width and depth of the bypass channel 4 are both 300 microns. Both the main channel 3 and the bypass channel 4 are square rectangular channels. The radial width of the constriction is 200 microns, and the main channel 3 has ten stages of constriction, and the constriction interval is 2000 microns. A polytetrafluoroethylene needle with an outer diameter of 200 microns and an inner diameter of 50 microns is inserted into the bypass channel 4 and protrudes toward the side of the main channel 3, and the front end of the capillary is flush with the upper side of the upstream main channel 3. The channel structure is shown in Figure 1. Both sides of the main channel adopt the dispersed phase injection method of injecting into the main channel 3 after being distributed by the upper distribution channel 6 (the structure on the upper side of Figure 1).

The continuous phase was injected using a polytetrafluoroethylene hose, and the two phases were collected using a quartz glass capillary. Add the n-octanol of oil-soluble surfactants (mass concentration is 0.1wt%-5wt%) such as span-20, span-40, span-60, span-80, span-85, tween-20 and tween-80 The solution is used as the continuous phase, and the aqueous solution dissolved with chitosan (the mass fraction in the dispersed phase is 1wt% to 5wt%) and acetic acid (the mass fraction in the dispersed phase is 1wt% to 5wt%) is used as the dispersed phase. The pump injects the dispersed phase into the upper distribution channel 6, and the dispersed phase fluid is injected into the bypass channel 4 after distribution. By adjusting the two-phase flow, the chitosan monomer droplet group with an average size of 30-100 microns can be obtained, and after thermally induced polymerization, monodisperse chitosan microspheres with an average size of 10-50 microns can be obtained. The microspheres have a complete structure and good stability.

It can be seen from the above examples that the present invention has the advantages of simple structure, convenient manufacture, not easy to clog, and greatly improves the generation frequency of monodisperse liquid droplets and bubbles. Micro-scale droplets and bubbles, especially suitable for the scale preparation of 1-100 micron micro-scale monodisperse droplets and bubbles and the batch scale-up of microsphere production.

The present invention can have a variety of specific implementations without departing from its spirit and essential characteristics. It should be understood that the above-mentioned embodiments are not limited to any of the above-mentioned details, but should be widely used within the spirit and scope defined by the appended claims. Therefore, all changes and modifications that come within the metes and bounds of the claims or are equivalent to such metes and bounds are therefore intended to be embraced in the appended claims.

Claims (10)

1. the micro-structural device that a kind of series connection is amplified, it is characterised in that the micro-structural device is logical by microchannel substrate (1), master Road (3) and bypass channel (4) composition；

The cross structure rectangular channel of series connection is set on the microchannel substrate (1) used as main channel (3), the one of main channel (3) Side or both sides are provided with bypass channel (4), and bypass channel (4) is perpendicular to main channel (3)；Bypass channel (4) is embedded in capillary tube, Capillary tube is closely coupled with microchannel substrate (1), and forms necking in intersection with main channel (3), and necking connects to be formed staggeredly Ladder-type structure；

Continuous phase fluid inlet tube (2), continuous phase fluid inlet tube (2) and microchannel are set at the upstream entrance of main channel (3) Substrate (1) sealing is connected；Two-phase fluid outlet (5), two-phase fluid outlet (5) are set at the lower exit of main channel (3) It is connected with microchannel substrate (1) sealing.

2. micro-structural device according to claim 1, it is characterised in that the bypass channel (4) for radially it is lower staggeredly Cascaded structure.

3. micro-structural device according to claim 1, it is characterised in that the bypass channel (4) is to interlock radially upward Cascaded structure.

4. micro-structural device according to claim 1, it is characterised in that the bypass channel (4) directly picks out microchannel Substrate (1).

5. micro-structural device according to claim 2, it is characterised in that the bypass channel (4) collect after Jing by higher level Assignment channel (6) picks out microchannel substrate (1).

6. micro-structural device according to claim 1, it is characterised in that main channel (3) corner is arc or right angle.

7. micro-structural device according to claim 1, it is characterised in that the width of the main channel (3) and bypass channel (4) Spend for 300～1300 μm, depth is 300～1300 μm；The capillary tube of the embedded bypass channel (4) is being handed over main channel (3) The radial width of the necking formed at remittance is 10～300 μm；The spacing of the necking is 500～5000 μm.

8. micro-structural device according to claim 1, it is characterised in that the series connection series of the necking series connection is 2～20 Level.

9. micro-structural device according to claim 1, it is characterised in that the capillary inner diameter is 50～1000 μm, outward Footpath is not less than bypass channel (4) and the depth of main channel (3)；Material is glass, rustless steel or politef.

10. micro-structural device according to claim 1, it is characterised in that the material of the microchannel substrate (1) is poly- first Base acrylic acid methyl ester., polydimethylsiloxane, glass, rustless steel, silicon chip, politef or photosensitive resin.