CN105055482A - Nutritional supplement and preparation method of soft capsule thereof - Google Patents
Nutritional supplement and preparation method of soft capsule thereof Download PDFInfo
- Publication number
- CN105055482A CN105055482A CN201510579596.XA CN201510579596A CN105055482A CN 105055482 A CN105055482 A CN 105055482A CN 201510579596 A CN201510579596 A CN 201510579596A CN 105055482 A CN105055482 A CN 105055482A
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- CN
- China
- Prior art keywords
- astaxanthin
- soft capsule
- natural vitamin
- safflower oil
- preparation
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- 238000002360 preparation method Methods 0.000 title claims abstract description 36
- 235000015872 dietary supplement Nutrition 0.000 title abstract description 11
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 76
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Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a nutritional supplement containing astaxanthin, natural vitamin E and safflower oil, belonging to the field of nutriment. The nutritional supplement comprises active ingredients such as the astaxanthin, the natural vitamin E and the safflower oil, meanwhile, the invention also provides a preparation method of a soft capsule by using the nutritional supplement. The nutritional supplement provided by the invention has the beneficial effects of improving the antioxidant capacity, scavenging free radical, increasing immunity and preventing cancer; the nutritional supplement is of dietary supplement with nutritional value; an astaxanthin soft capsule provided by the invention adopts a new dosage form production process, has the beneficial effects that the absorption rate is high, the bioavailability is high, the astaxanthin, the natural vitamin E and the safflower oil can cooperate with each other for resisting oxidation and preventing lipid peroxidation, the effect of strengthening the immune system is greater than the effect of individual components, and the effect of the ratio of the appropriate proportion of the astaxanthin, the natural vitamin E and the safflower oil is greater than the effect of the sum of the astaxanthin, the natural vitamin E and the safflower oil in terms of oxidation resistance.
Description
Technical field
The present invention relates to a kind of supplementary, particularly relate to the supplementary of a kind of astaxanthin-containing, natural Vitamin E Flos Carthami seed oil, belong to nutrient field.
Background technology
Along with improving constantly of health of people consciousness, more focus on nutrition and quality, but modern's operating pressure is large, ecological deterioration, cause interior free yl too much, body aging phenomenon is serious, and hypoimmunity, is in sub-health state.Astaxanthin can remove interior free yl, slow down aging, useful joint, enhancing immunity, useful eyes and brain, stamina intensifying, defying age; Natural Vitamin E has scavenging free radicals, antidotal effect, and the effects such as enhancing immunity, prevent arteriosclerosis, keeps the effects such as muscle, neural blood vessel, hemopoietic system normal function; Safflower oil is rich in linoleic acid, has and prevents cholesterol from depositing in blood vessel wall, atherosclerosis, prevention and cure of cardiovascular disease and hypotensive activity.
In prior art, many inventions propose the compound mode adopting astaxanthin, vitamin and oils to produce supplementary: if publication number is the application of CN101023963A, point out natural astaxanthin 0.5-10 part, bathypelagic fish liver oil 1-85 part, natural Vitamin E 0.1-1 part in " a kind of preparation method of natural astaxanthin and fishliver oil ", and adopt above-mentioned deal to be mixed with natural astaxanthin and fishliver oil.Publication number is the application of CN101053409A, point out in " health food containing natural astaxanthin " astaxanthin-containing weight be 5% natural astaxanthin oil 2.1-16 part, edible fats 80-95 part, Natural antioxidant 0.001-0.5 part, make liquid capsule.Publication number is the application of CN102793184A, point out containing astaxanthin, coenzyme Q10, vitamin E in compound antioxidant nutrition supplement in " a kind of compound antioxidant nutrition supplement and processing method thereof ", and in the course of processing, above-mentioned three kinds of mixture are added edible oil and fat and carry out oily molten process.These patents provide some reference schemes for adopting astaxanthin, vitamin and oils to produce supplementary, but, auxiliary or the description of test that in these applications, pointed concrete formulation components and amount of preparation etc. are not relevant, do not point out the relevant functional place of this component, estimate its preparation and have certain random or random yet; We is found by test of many times simultaneously, although the compositions of above-mentioned three invention gained has certain non-oxidizability really, its effect is not fully up to expectations.
In addition, the preparation technology of astaxanthin capsule product is mainly with low-temperature heat in the market, is mixed into master for a long time, causes effect of functional component to reduce, functional component does not have scientific matching, the scientific validation that the actual effect of functional component and edible safety are also not relevant and pertinent literature report.
Summary of the invention
For above-mentioned defect, the present invention, by large quantifier elimination and experiment, provides a kind of supplementary, and for improving oxidation resistance, scavenging free radicals, raising immunity, it has good effect at antioxidation and reduction lipid aspects; The present invention also provides the soft capsule adopting described supplementary to prepare simultaneously, and the preparation method of soft capsule.
The effective ingredient of described supplementary is astaxanthin, natural Vitamin E Flos Carthami seed oil.
Described supplementary, each effective ingredient weight is: astaxanthin 1-10, natural Vitamin E 0.5-1.5, safflower oil 30-80.
Described supplementary, preferred each effective ingredient weight is: astaxanthin 6-8, natural Vitamin E 0.8-1.2, safflower oil 30-50.
Described supplementary, more preferably each effective ingredient weight is: astaxanthin 8, natural Vitamin E 1, safflower oil 41.
Described supplementary can be prepared as soft capsule, also needs to add adjuvant during preparation, and adjuvant is: gelatin, purified water and glycerol; Described adjuvant mixes by a certain percentage, plays capsule clothing encapsulation action.
Described soft capsule, each ingredients weight parts proportioning is: effective ingredient 45-55, gelatin 10-20, glycerol 5-10, purified water 10-20;
Described soft capsule, preferred each ingredients weight parts proportioning is: effective ingredient 50, gelatin 15, glycerol 7.5, purified water 15.
The preparation method of described soft capsule, comprise colloidal sol, the preparation of content feed liquid, pelleting, shape, wash ball, drying, select ball, inner packing, outer package, inspection and finished product warehouse-in:
1 colloidal sol
Get gelatin and add appropriate purified water, soak and make it expand, obtain expansion gelatin; By glycerol and the heating of remaining purified water, add expansion gelatin and the heating that heats up, stirring and dissolving mixes, and constant temperature leaves standstill, and goes offscum, vacuumizing and defoaming, adopts screen filtration, is incubated for subsequent use, obtains glue.
Particularly, preferably get the purified water that gelatin adds 30%-50%, soak and make it expand, obtain expansion gelatin; Glycerol and remaining purified water are heated to 55-65 DEG C, are preferably heated to 60 DEG C, add expansion gelatin and be heated to 70-80 DEG C, stirring and dissolving mixes, constant temperature leaves standstill 1-2 hour, goes offscum, vacuumizing and defoaming, adopts screen filtration, preferably 100 eye mesh screens, 60-65 DEG C of insulation is for subsequent use, obtains glue.
Adopting two-step method to add purified water in this step can make gelatin expansion effect better, and plasticity and the elasticity of cyst wall are more excellent.
2 content feed liquid preparations
Get appropriate safflower oil, mix with natural Vitamin E, obtain natural Vitamin E fluid; By astaxanthin, natural Vitamin E fluid, remaining safflower oil mix homogeneously, heating, stirring, obtain content feed liquid.
Particularly, preferably get 30%-50% safflower oil, mix with natural Vitamin E, obtain natural Vitamin E fluid; By astaxanthin, natural Vitamin E fluid, remaining safflower oil mix homogeneously, be heated to 60-70 DEG C, be preferentially heated to 65 DEG C, stir 0.25-1 hour, preferential stirring 0.45 hour, obtains content feed liquid.
Adopt in this step two-step method add safflower oil make contents mixed evenly, create the solution environmental of natural Vitamin E simultaneously, protection astaxanthin avoid oxidized; Suitably improve heating-up temperature, simultaneously do not affecting under content material stability prerequisite, improve mixing homogeneity, shorten mixing time, can under the full and uniform prerequisite of guarantee, avoid content due to homogenizing time long and cause loss.
3 pelletings
Content feed liquid and glue are carried out pelleting.During pelleting, preferably control indoor temperature at 22-24 DEG C, relative humidity is at 40-45%.Carry out pelleting according to pelleting specification, preferably adopt pelleting specification to be 0.5g/ grain.
4 sizings
By compressing soft capsule sizing.Preferably in temperature at 22-24 DEG C, relative humidity is dry 2.5-4 hour under 40-45%, preferably dry 4 hours.
At this temperature and relative humidity, soft capsule sizing is stablized, not yielding.
5 wash ball
Soft capsule ethanol purge after sizing, preferred alcohol concentration is 95%.
6 is dry
Soft capsule after cleaning is carried out drying.Preferably at temperature 26-28 DEG C time dry, the clean dried indoor of relative humidity 30-40% are carried out, static dry 24 hours.
At this temperature and relative humidity, carry out drying, soft capsule is shaped easily, and not easily breaks, and becomes ball rate higher.
7 select ball
Reject underproof useless balls such as being out of shape, break, retain qualified soft capsule.
8 inner packings
Inner packing adopts oral stable medicinal polythene bottle with high density (standard No. YBB00122002), packing specification: 60/bottle.
9 outer package
Outer package adopts corrugated case.
10 inspections
Test by company standard inspection item.
11 finished product warehouse-ins
Examination and test of products warehousing after passing.
Astaxanthin (Astaxanthin) is a kind of dietary supplement, belong to carotenoid, be prevalent in a lot of plant especially algae, in phytoplankton, also be present in minority antibacterial and fungus, it is a kind of chain breaking antioxidants, there is antioxidation, scavenging free radicals, stop lipid peroxidation, slow down aging, prevention and prohibition cancer, prevention of arterial hardens, enhancing immunity, safeguard the effect such as eyes and central nervous system's health, the immunologic function of astaxanthin energy appreciable impact animal, when there being antigen to produce, obvious promotion splenocyte produces antibody, the immunocompetence of obvious enhancing body local and whole body, opposing body inflammatory, increase the vigor of immune system B cell, make body can eliminate the pathogen of external source invasion, promote the generation of human immunoglobulin, there is higher immunoregulatory activity.
Natural Vitamin E (VitaminE) is also a kind of dietary supplement; contribute to improving endurance; reduce cell oxygen consumption, anti-oxidation protection body cell, from free radical damage, improves lipid metabolism; prevention coronary heart disease and atherosclerosis; prevention of inflammation, improves blood circulation, reduces cholesterol; prophylaxis of hypertension, prevention and treatment varicosis.
Safflower oil (saffloweroil) is a kind of dietary supplement, wherein the highest containing linoleic acid ratio, the vitamin E in oil, oryzanol, sterol nutritional labeling, can effectively harden and reduce blood cholesterol levels by prevention of arterial, remove blood vessel deposit, reduce blood fat.
Astaxanthin, natural Vitamin E Flos Carthami seed oil three has collaborative antioxidation, prevents lipid peroxidation, enhancing immunity, effect of common resisting fatigue, enhancing body vigor, the effect that three share is greater than separate constituent role, and the proportioning of proper ratio plays the effect being greater than addition in antioxidation.
Compared with prior art, beneficial effect of the present invention is:
(1) soft capsule of supplementary of the present invention and preparation thereof, in production technology, the cycle is shorter, effectively can reduce the degradation rate of effect material, and product quality parameters improves, and can promote that content better absorbs.
(2) supplementary of the present invention can improve oxidation resistance, scavenging free radicals, raising immunity, is the dietary supplement with nutritive value.
(3) astaxanthin, natural Vitamin E Flos Carthami seed oil are worked in coordination with antioxidation and prevent the action effect of lipid peroxidation to be greater than separate constituent role, and the proportioning of proper ratio plays the effect being greater than addition in antioxidation.
(4) soft capsule made with proper ratio of astaxanthin, natural Vitamin E Flos Carthami seed oil is through the animal experiment of clinical efficacy and safety testing, and result shows to have enhancing immunity effect, has safety in utilization.
Detailed description of the invention
Below in conjunction with embodiments such as the preparation method of supplementary soft capsule, the antioxidation experiment of supplementary and effect for reducing blood fat experiments, full and accurate elaboration is carried out to the present invention.
Embodiment 1: supplementary soft capsule prepares 1
Described soft capsule, each ingredients weight parts proportioning is: astaxanthin oil 8kg, natural Vitamin E 1kg, safflower oil 41kg, gelatin 15kg, glycerol 7.5kg, purified water 15kg.
The preparation process of soft capsule is:
(1) colloidal sol
Get gelatin add 7.5kg purified water soak make it expand, obtain expansion gelatin; Glycerol and remaining purified water are heated to 60 DEG C, add expansion gelatin and be heated to 70 DEG C, stirring and dissolving mixes, and constant temperature leaves standstill 1.5 hours, goes offscum, vacuumizing and defoaming, and adopt 100 eye mesh screens to filter, 60 DEG C of insulations are for subsequent use, obtain glue.
(2) content feed liquid preparation
Get 20kg safflower oil, mix with natural Vitamin E, obtain natural Vitamin E fluid; By astaxanthin, natural Vitamin E fluid, remaining safflower oil mix homogeneously, be heated to 65 DEG C, stir 0.45 hour, obtain content feed liquid.
(3) pelleting
Content feed liquid and glue are carried out pelleting, and control indoor temperature at 23 DEG C, relative humidity is 42%.Pelleting specification is 0.5g/ grain.
(4) shape
By compressing soft capsule sizing, dry 4 hours.Control indoor temperature at 23 DEG C, relative humidity is 42%.
(5) ball is washed
Soft capsule after sizing 95% ethanol purge.
(6) dry
Soft capsule after cleaning is placed in temperature 26 DEG C, and the clean dried of relative humidity 35% is indoor, static dry 24 hours.
(7) ball is selected
Reject underproof useless balls such as being out of shape, break, retain qualified soft capsule.
Follow-up again through inner packing, outer package, inspection and finished product warehouse-in, complete whole production procedure.
Embodiment 2: supplementary soft capsule prepares 2
In described soft capsule, each ingredients weight parts proportioning is: astaxanthin oil 6kg, natural Vitamin E 1.5kg, safflower oil 72kg, gelatin 23kg, glycerol 11kg, purified water 20kg.
The preparation process of soft capsule is:
(1) colloidal sol
Get gelatin add 10kg purified water soak make it expand, obtain expansion gelatin; Glycerol and remaining purified water are heated to 60 DEG C, add expansion gelatin and be heated to 75 DEG C, stirring and dissolving mixes, and constant temperature leaves standstill 2 hours, goes offscum, vacuumizing and defoaming, and adopt 100 eye mesh screens to filter, 65 DEG C of insulations are for subsequent use, obtain glue.
(2) content feed liquid preparation
Get 25kg safflower oil, mix with natural Vitamin E, obtain natural Vitamin E fluid; By astaxanthin, natural Vitamin E fluid, remaining safflower oil mix homogeneously, be heated to 70 DEG C, stir 0.3 hour, obtain content feed liquid.
(3) pelleting
Content feed liquid and glue are carried out pelleting, and control indoor temperature at 22 DEG C, relative humidity is 40%.Carry out pelleting according to pelleting specification, adopt pelleting specification to be 0.5g/ grain.
(4) shape
By compressing soft capsule sizing, dry 3 hours.Control indoor temperature at 24 DEG C, relative humidity is 45%.
(5) ball is washed
Soft capsule after sizing 95% ethanol purge.
(6) dry
Soft capsule after cleaning is placed in temperature 26 DEG C, and the clean dried of relative humidity 35% is indoor, static dry 24 hours.
(7) ball is selected
Reject underproof useless balls such as being out of shape, break, retain qualified soft capsule.
Follow-up again through inner packing, outer package, inspection and finished product warehouse-in, complete whole production procedure.
Embodiment 3: supplementary soft capsule prepares 3
In described soft capsule, each ingredients weight parts proportioning is: astaxanthin oil 1kg, natural Vitamin E 0.5kg, safflower oil 80kg, gelatin 25kg, glycerol 15kg, purified water 25kg.
The preparation process of soft capsule is:
(1) colloidal sol
Get gelatin add 12kg purified water soak make it expand, obtain expansion gelatin; Glycerol and remaining purified water are heated to 65 DEG C, add expansion gelatin and be heated to 80 DEG C, stirring and dissolving mixes, and constant temperature leaves standstill 1 hour, goes offscum, vacuumizing and defoaming, and adopt 100 eye mesh screens to filter, 60 DEG C of insulations are for subsequent use, obtain glue.
(2) content feed liquid preparation
Get 32kg safflower oil, mix with natural Vitamin E, obtain natural Vitamin E fluid; By astaxanthin, natural Vitamin E fluid, remaining safflower oil mix homogeneously, be heated to 60 DEG C, stir 1 hour, obtain content feed liquid.
(3) pelleting
Content feed liquid and glue are carried out pelleting, and control indoor temperature at 24 DEG C, relative humidity is 45%.Carry out pelleting according to pelleting specification, adopt pelleting specification to be 0.5g/ grain.
(4) shape
By compressing soft capsule sizing, dry 4 hours.Control indoor temperature at 22 DEG C, relative humidity is 40%.
(5) ball is washed
Soft capsule after sizing 95% ethanol purge.
(6) dry
Soft capsule after cleaning is placed in temperature 28 DEG C, and the clean dried of relative humidity 40% is indoor, static dry 24 hours.
(7) ball is selected
Reject underproof useless balls such as being out of shape, break, retain qualified soft capsule.
Follow-up again through inner packing, outer package, inspection and finished product warehouse-in, complete whole production procedure.
Embodiment 4: the antioxidation test of supplementary
1. materials and methods
Astaxanthin, natural Vitamin E, safflower oil, to distill water as solvent preparation tested material during test.
2 instruments and reagent
2.1 instruments: microplate reader, ten thousand/balance, 96 hole microwell plates, liquid-transfering gun; Glass drying oven: volumetric flask is some, beaker, pipet.
2.2 reagent: dipotassium hydrogen phosphate, sodium dihydrogen phosphate, uranine yellow salt, Trolox standard substance, AAPH solution and distilled water.
3 sample determinations
Get the 1-7 group sample described in table 1 and standard polyphenoils (Trolox) respectively, with dipotassium hydrogen phosphate, phosphate sodium dihydrogen buffer solution, it is diluted, get each 20 μ L of diluent respectively, after mixing with 160 μ L uranine yellow saline solution respectively, 30min is incubated at 37 DEG C, and then add AAPH solution 20 μ L, start rapidly to measure, use microplate reader record fluorescence signal, obtain the standard curve of antioxidant concentration and fluorescence signal intensity, the antioxidation index (ORAC) of each sample is obtained by standard curve; The result (referring to table 1) of the antioxidation index (ORAC) finally obtained and the dilution factor of sample have nothing to do, only relevant with the relative concentration between each component.
As shown in table 1, wherein: A represents astaxanthin, B1 represents natural Vitamin E, and B2 represents vitamin C, and C1 represents safflower oil, and C2 represents cod-liver oil, and C3 represents Oleum Arachidis hypogaeae semen, and C4 represents coenzyme Q10+Oleum Glycines; Choosing of above-mentioned substance (B2, C2, C3 and C4) derives from several application documents mentioned in background technology, adopts these data and the present invention to analyze.Adopt SPSS to do one factor analysis of variance, tested number 1-7 is formula contrast groups, and in table, the data of A, B, C refer to the weight portion of each component, and 1-3 number is chosen according to the weight provided in embodiment of the present invention 1-3.
Table 1 EXPERIMENTAL DESIGN table
| Tested number | A | B | C | ORAC |
| 1 | 8 | B1＝1 | C1＝41 | 901.3 |
| 2 | 6 | B1＝1.5 | C1＝72 | 820.7 |
| 3 | 1 | B1＝0.5 | C1＝80 | 789.5 |
| 4 | 1 | B2＝0.5 | C1＝80 | 532.5 |
| 5 | 1 | B1＝0.5 | C2＝80 | 657.6 |
| 6 | 1 | B1＝0.5 | C3＝80 | 407.8 |
| 7 | 1 | B1＝0.5 | C4＝5+75 | 551.0 |
4 data statistic analysis
Adopt SPSS17.0 statistical software to carry out one factor analysis of variance (One-WayANOVA), during significant difference (P < 0.05) between single process, carry out multiple comparison analyse with LSD inspection.
5 results and analysis
Table 2 test of normality
P > 0.05 as shown in Table 2, shows that parametric test meets normality distributional assumption, can carry out subsequent analysis.
Table 3 homogeneity test of variance
ORAC value
| Levene statistic | df1 | df2 | Significance (P) |
| 3.239 | 6 | 14 | .063 |
P > 0.05, shows homogeneity of variance as shown in Table 3, meets null hypothesis, can carry out subsequent analysis.
Table 4 one factor analysis of variance
ORAC value
The P value < 0.05 of F inspection between group, shows that the diversity between factor process has significance, has statistical significance, can carry out statistical analysis as shown in Table 4.
The variance analysis of table 5 multiple comparisons
ORAC value
LSD
*. all the significance level of value difference is 0.05.
The ORAC value of table 6 control variable
By the class mean in the similar subset of display.
A. harmomic mean sample size=3.000 will be used.
B. Class1/type 2 error severity ratio=100.
From table 5 and table 6, comparative result between two between process, control variable is that to be greater than control variable be ascorbic antioxidant effect to the group antioxidant effect of natural Vitamin E; Control variable is the antioxidant effect that the antioxidant effect of the group of safflower oil is better than that control variable is cod-liver oil, Oleum Arachidis hypogaeae semen and coenzyme Q10+Oleum Glycines.This experiment shows that astaxanthin, natural Vitamin E Flos Carthami seed oil has synergism in oxidation and removing free radicals enhancing immunity, and when astaxanthin be 8 parts, vitamin E is 1 part, safflower oil is 41 parts time, the antioxidant effect of described supplementary is best.
Embodiment 5: the effect for reducing blood fat test of supplementary
1. materials and methods
Astaxanthin, natural Vitamin E, safflower oil, vegetable oil, cod-liver oil, Petiolus Trachycarpi oil; To distill water as solvent preparation tested material during test.
2 instruments and reagent
2.1 instruments: microplate reader, refrigerated centrifuger, ten thousand/balance, vortex oscillation instrument, 96 hole microwell plates, liquid-transfering gun; Glass drying oven: volumetric flask is some, beaker, pipet.
2.2 reagent: triglyceride determination test kit, T-CHOL measures test kit, normal feedstuff, Adeps Sus domestica, yolk powder, cholesterol, sodium cholate, CMC-Na.
3 sample determinations
The foundation of 3.1 hyperlipidemia models
(1) high lipid food formula: 80% normal feedstuff, 10% Adeps Sus domestica, 5% yolk powder, 4.5% cholesterol, 0.5% sodium cholate.Wherein: in normal feedstuff, each component ratio is: Semen Maydis powder 27%, wheat bran 19%, rice 16%, soybean cake 16%, fish flour 13%, calcium powder 3%, bone meal 3%, yeast powder 2.3%, Sal 0.5%, compound vitamin 0.1%, trace element 0.1%.
(2) regular grade male mice 60 is got, body weight 15-20g.Conventional raising 1 week, gets 10 at random as blank group, feeds normal feedstuff; Feed high lipid food, give distilled water, modeling one month for all the other 50.
3.2 administration designs
Proportioning of throwing something and feeding is prepared by table 1, according to the feeding volume feeding of 1.5g (kg/d); 10 mices of former normal feedstuff of throwing something and feeding are continued as blank group (No. 8 groups) normal feedstuff of throwing something and feeding, feeds the mice of high lipid food from 50 and choose 21 successful mices of modeling, it is divided into 7 groups at random, often organizes 3; Using the mice of wherein one group of former high lipid food of throwing something and feeding as positive control hyperlipidemia group (No. 7 groups), start normal feedstuff of throwing something and feeding; Remain 6 groups of hyperlipidemia mices (tested number 1-6) to throw something and feed and add the normal feedstuff of supplementary, supplementary weight accounting is 15%.
3.3 assay method
Each group continues to throw something and feed 5 days, gets blood 2ml respectively, leaves standstill 1 hour, centrifugal 10 minutes, gets supernatant and obtain serum sample, measures the content of Triglycerides in Serum and T-CHOL by test kit operation instruction.
As shown in table 1, wherein: A represents astaxanthin, C represents natural Vitamin E, and B1 represents safflower oil, and B2 represents cod-liver oil, and B3 represents vegetable oil, and B4 represents Petiolus Trachycarpi oil; Tested number 1,2,3 represents the experimental group adopting astaxanthin, natural Vitamin E Flos Carthami seed oil to throw something and feed, tested number 4,5,6 represents respectively compared with the 3rd group, different and other the consistent contrast tests of edible oil and fat, tested number 7 is positive control hyperlipidemia group, and tested number 8 is the blank group of negative control.
Adopt SPSS to do one factor analysis of variance, tested number 1-6 is formula contrast groups, and in table, the data of A, B, C refer to the weight portion of each component, and the weight according to providing in summary of the invention is chosen.
Table 7 EXPERIMENTAL DESIGN table
4 data statistic analysis
Adopt SPSS17.0 statistical software to carry out one factor analysis of variance (One-WayANOVA), during significant difference (P < 0.05) between single process, carry out multiple comparison analyse with LSD inspection.
5 results and analysis
The test of normality of table 8 total cholesterol level
P > 0.05 as shown in Table 8, shows that parametric test meets normality distributional assumption, can carry out subsequent analysis.
The homogeneity test of variance of table 9 total cholesterol level
Control variable
| Levene statistic | df1 | df2 | Significance (P) |
| .424 | 7 | 16 | .873 |
P > 0.05, shows homogeneity of variance as shown in Table 9, meets null hypothesis, can carry out subsequent analysis.
The one factor analysis of variance of table 10 total cholesterol level
Control variable
The P value < 0.05 of F inspection between group, shows that the diversity between factor process has significance, has statistical significance, can carry out statistical analysis as shown in Table 10.
The multiple comparisons of table 11 total cholesterol level
Dependent variable: control variable
*. all the significance level of value difference is 0.05.
The multiple comparisons of table 12 total cholesterol level
By the class mean in the similar subset of display.
A. harmomic mean sample size=3.000 will be used.
B. Class1/type 2 error severity ratio=100.
From table 11 and table 12, comparative result between two between process, control variable is that the effect of the group reduction cholesterol level of safflower oil is greater than the group that control variable is fish oil, and reach the effect of normal feedstuff without hyperlipidemia group (No. 8) of only throwing something and feeding, reduce the effect of total cholesterol level in turn for cod-liver oil is greater than vegetable oil simultaneously, vegetable oil is greater than Petiolus Trachycarpi oil, all be greater than hyperlipidemia group, show that the diversity that safflower oil group is reducing total cholesterol level in blood reaches remarkable, its effect is better than other edible oil and fat; Simultaneously when astaxanthin be 8 parts, vitamin E is 1 part, safflower oil is 41 parts time proportioning component time, described supplementary reduces the best results of cholesterol level.
The test of normality of table 13 content of triglyceride
P > 0.05 as shown in Table 13, shows that parametric test meets normality distributional assumption, can carry out subsequent analysis.
The homogeneity test of variance of table 14 content of triglyceride
Control variable
| df1 | df2 | Significance (P) |
| 7 | 16 | .873 |
P > 0.05, shows homogeneity of variance as shown in Table 14, meets null hypothesis, can carry out subsequent analysis.
The one factor analysis of variance of table 15 total cholesterol level
Control variable
The P value < 0.05 of F inspection between group, shows that the diversity between factor process has significance, has statistical significance, can carry out statistical analysis as shown in Table 15.
The multiple comparisons of table 16 content of triglyceride
Dependent variable: control variable
*. all the significance level of value difference is 0.05.
The multiple comparisons of table 17 content of triglyceride
By the class mean in the similar subset of display.
A. harmomic mean sample size=3.000 will be used.
B. Class1/type 2 error severity ratio=100.
From table 16 and table 17, comparative result between two between process, control variable is that the effect of the group reduction content of triglyceride of safflower oil is all greater than the group that control variable is other oils and fatss, reduce the effect of content of triglyceride in turn for cod-liver oil is greater than vegetable oil simultaneously, vegetable oil is greater than Petiolus Trachycarpi oil, all be greater than hyperlipidemia group, show that the diversity that safflower oil group is reducing content of triglyceride in blood reaches remarkable, its effect is better than other edible oil and fat; Simultaneously when astaxanthin be 8 parts, vitamin E is 1 part, safflower oil is 41 parts time proportioning component time, described supplementary reduces the best results of content of triglyceride.
In sum, add the effect of natural Vitamin E Flos Carthami seed oil in antioxidation in the soft capsule of supplementary and preparation thereof and be better than other antioxidants, add the effect that safflower oil is removing T-CHOL and triglyceride reduction blood fat simultaneously and be better than other edible oil and fat.Show with astaxanthin, natural Vitamin E, the supplementary that safflower oil is made by a certain percentage has effect of scavenging free radicals, antioxidation, enhancing immunity and reduction blood fat.
Embodiment: 6: supplementary soft capsule preparation technology parameter is on the impact of content viscosity of sludge
1. materials and methods
Astaxanthin oil, natural Vitamin E, safflower oil.
2 instruments and reagent
2.1 instruments: falling ball method viscosity coefficient investigating instrument, laser photoelectricity chronograph, graduated cylinder, thermometer, small ball, stopwatch, slide calliper rule, micrometer etc.
3 sample determinations
3.1 adjustment coefficient of viscosity measuring device and test apparatuses
Adjustment chassis level, switch on power and adjusting laser beam, test stand puts steel ball conduit, and bead alcohol washes also blots.
3.2 contrast experiments: get 20 parts of safflower oils, mix with 1 part of natural Vitamin E, obtain natural Vitamin E fluid; By 8 parts of astaxanthins, natural Vitamin E fluid, remaining 21 parts of safflower oil mix homogeneously, be heated to 50 DEG C, stir 1 hour, obtain content feed liquid, as sample A, parallel sample is A1A2A3.
3.3 the present invention: get 20 parts of safflower oils, mix with 1 part of natural Vitamin E, obtain natural Vitamin E fluid; By 8 parts of astaxanthins, natural Vitamin E fluid, remaining 21 parts of safflower oil mix homogeneously, be heated to 65 DEG C, stir 0.45 hour, obtain content feed liquid, as sample B, parallel sample is B1B2B3.The spacing L of the upper and lower two laser of 3.4 measurement
Bead is put into steel ball conduit by 3.5, and bead falls, and stops laser beam above, manual time-keeping, when falling the red laser beam below stop to bead, stops timing, reads fall time, repeat 6 times.
3.6 calculation sample A and the sample B coefficient of viscosity.
4 data statistic analysis
SPSS17.0 statistical software is adopted to carry out one factor analysis of variance (One-WayANOVA), using α=0.05 as significance level, each group of homogeneity of variance then adopts student-newman-keuls method to carry out mean to compare, homogeneity of variance can not meet, and adopts other statistical method as games-Howell inspection etc.
5 interpretations of result
Table 18 one factor analysis of variance table
Viscosity number
Can be obtained by table 18, the p value < 0.05 of F inspection between group, the diversity between the combination showing different ratio reaches extremely significantly, has statistical significance, can carry out statistical analysis.
Sample A and sample B there are differences in solution temperature and mixing time, experiment shows that the coefficient of viscosity of sample B and sample A has significant difference, show to improve and dissolve mixing temperature, shorten mixing time and have significance effect to the coefficient of viscosity reducing soft capsule content liquid, and the reduction of the coefficient of viscosity is for the utilization rate improving functional component, increase the uniformity of composition mixing, improve production technology quality aspect and play an important role.
Embodiment 7: supplementary soft capsule preparation technology parameter and technological process are on the impact of content feed liquid non-oxidizability
1. materials and methods
Astaxanthin oil, natural Vitamin E, safflower oil.
2 instruments and reagent
2.1 instruments: ultraviolet spectrophotometer, analytical balance, centrifuge etc.
2.2 reagent: acetone, distilled water etc.
3 sample determinations
3.1 according to ultraviolet spectrophotometer technical manual, and debugging machine is to duty.
3.2 cleaning volumetric flask used, beaker, shears, tweezers and conduction sticks.
3.3 determined wavelength 474nm
3.4 contrast experiments 1: get 20 parts of safflower oils, mix with 1 part of natural Vitamin E, obtain natural Vitamin E fluid; By 8 parts of astaxanthins, natural Vitamin E fluid, remaining 21 parts of safflower oil mix homogeneously, be heated to 50 DEG C, stir 1 hour, obtain content feed liquid, as sample A, parallel sample is A1A2A3.
3.5 contrast tests 2: by natural astaxanthin by natural astaxanthin 8 parts, natural Vitamin E 1 part, safflower oil 41 parts is placed in reactor and is heated to 65 DEG C, stirs 0.45 hour, obtains content feed liquid, and as sample B, parallel sample is B1B2B3.
3.6 the present invention: get 20 parts of safflower oils, mix with 1 part of natural Vitamin E, obtain natural Vitamin E fluid; By 8 parts of astaxanthins, natural Vitamin E fluid, remaining 21 parts of safflower oil mix homogeneously, be heated to 65 DEG C, stir 0.45 hour, obtain content feed liquid, as sample C, parallel sample is C1C2C3.
With acetone solution to suitable extension rate, to be measured.
3.7 measure absorbance according to formulae discovery content astaxanthin.
4 data statistic analysis
SPSS17.0 statistical software is adopted to carry out one factor analysis of variance (One-WayANOVA), using α=0.05 as significance level, each group of homogeneity of variance then adopts student-newman-keuls method to carry out mean to compare, homogeneity of variance can not meet, and adopts other statistical method as games-Howell inspection etc.
5 interpretations of result
Table 19 test of normality
P > 0.05 as shown in Table 19, shows that parametric test meets normality distributional assumption, can carry out subsequent analysis.
Table 20 homogeneity test of variance
Numerical value
| Levene statistic | df1 | df2 | Significance |
| 1.333 | 2 | 6 | .332 |
P > 0.05, shows homogeneity of variance as shown in Table 20, meets null hypothesis, can carry out subsequent analysis.
Table 21 multiple comparisons
Dependent variable: numerical value
*. all the significance level of value difference is 0.05.
Table 22 numerical value
By the class mean in the similar subset of display.
A. harmomic mean sample size=3.000 will be used.
B. Class1/type 2 error severity ratio=100.
Can be obtained by table 21 and table 22, sample A and sample C there are differences in solution temperature and mixing time, experiment shows: sample C has significant difference than the significant functional component astaxanthin degraded of sample A, show to improve and dissolve mixing temperature, shorten mixing time and have significance effect to the degradation rate reducing soft capsule content, astaxanthin is oxidized, to photo-thermal air-sensitive, over-exposure is under air and illumination, the content of astaxanthin can be caused to reduce, thus cause the functional component of supplementary to run off, weaken the oxidation and removing free radicals of functional component and the effect of enhancing immunity.
Simultaneously; sample B and sample C there are differences in technological process; what sample B adopted is the mode that gradation adds; experiment shows: sample C has significant difference than the significant functional component astaxanthin degraded of sample B; the solution environmental adopting natural Vitamin E is described; protecting astaxanthin avoids oxidized, has significance effect to the degradation rate reducing soft capsule content.
Embodiment 8: the soft capsule safety testing of supplementary and preparation thereof
Acute toxicity testing: the acute toxicity test of supplementary soft capsule is greater than 18.6g/ (kgBW) to female male rat acute toxicity maximum tolerated dose (MTD).
Genetic toxicity test: Salmonella reversion test, mice bone marrow micronucleus, mouse inbred strain result is showed no soft capsule mutagenic action.
30 days feeding trials: have no soft capsule to the weight of animals, food utilization, routine blood test, blood biochemistry, organ weights, dirty body ratio and histopathologic examination's result have harmful effect.
Embodiment 9: the significant component and effect test of soft capsule of supplementary and preparation thereof
Give the soft capsule 30 days of mice various dose, high low dosage can improve mouse spleen/body weight ratio, low dosage strengthens lymphocytic multiplication capacity, antibody-producting cell number, middle dosage can improve mice half hemolysis value, and high dose strengthens mouse monokaryon-macrophage carbonic clearance ability, macrophage phagocytic chicken red blood cell ability.Have no the impact of natural astaxanthin soft capsule on weight gain value, thymus/body weight ratio, delayed allergy (DTH), NK cytoactive.Meet " health food inspection and assessment technical specification-2003 " to judge about the result of enhancing immunity function.
Claims (10)
1. a supplementary, is characterized in that: effective ingredient is astaxanthin, natural Vitamin E Flos Carthami seed oil, and each effective ingredient weight is: astaxanthin 1-10, natural Vitamin E 0.5-1.5, safflower oil 30-80.
2. supplementary according to claim 1, is characterized in that: each effective ingredient weight is: astaxanthin 6-8, natural Vitamin E 0.8-1.2, safflower oil 30-50.
3. supplementary according to claim 1, is characterized in that: each effective ingredient weight is: astaxanthin 8, natural Vitamin E 1, safflower oil 41.
4. the soft capsule adopting the arbitrary described supplementary of claim 1-3 to prepare, is characterized in that: also need during preparation to add adjuvant, adjuvant is: gelatin, glycerol and purified water.
5. soft capsule according to claim 4, is characterized in that: each ingredients weight parts proportioning is: effective ingredient 45-55, gelatin 10-20, glycerol 5-10, purified water 10-20.
6. a preparation method for the arbitrary described soft capsule of claim 4 or 5, comprises colloidal sol, the preparation of content feed liquid, the large step of pelleting three, it is characterized in that:
Content feed liquid preparation process is: get appropriate safflower oil, mix with natural Vitamin E, obtains natural Vitamin E fluid; By astaxanthin, natural Vitamin E fluid, remaining safflower oil mix homogeneously, heating, stirring, obtain content feed liquid.
7. the preparation method of soft capsule according to claim 6, is characterized in that:
Content feed liquid preparation process is: get 30%-50% safflower oil, mix with natural Vitamin E, obtains natural Vitamin E fluid; By astaxanthin, natural Vitamin E fluid, remaining safflower oil mix homogeneously, be heated to 60-70 DEG C, stir 0.25-1 hour, obtain content feed liquid.
8. the preparation method of soft capsule according to claim 6, is characterized in that:
Sol-process is: get gelatin and add appropriate purified water, soaks and makes it expand, obtain expansion gelatin; By glycerol and the heating of remaining purified water, add expansion gelatin and the heating that heats up, stirring and dissolving mixes, and constant temperature leaves standstill, and goes offscum, vacuumizing and defoaming, adopts screen filtration, is incubated for subsequent use, obtains glue.
9. the preparation method of soft capsule according to claim 8, is characterized in that:
Sol-process is: get the purified water that gelatin adds 30%-50%, soaks and makes it expand, obtain expansion gelatin; Glycerol and remaining purified water are heated to 55-65 DEG C, add expansion gelatin and be heated to 70-80 DEG C, stirring and dissolving mixes, and constant temperature leaves standstill 1-2 hour, goes offscum, vacuumizing and defoaming, and adopt screen filtration, 60-65 DEG C of insulation is for subsequent use, obtains glue.
10. the preparation method of soft capsule according to claim 6, is characterized in that: also comprise sizing, wash ball, drying, select the step of ball;
Type-approval process is: by compressing soft capsule sizing, in temperature at 22-24 DEG C, relative humidity under 40-45%, dry 2.5-4 hour;
Washing ball process is: the soft capsule ethanol purge after sizing;
Dry run is: the soft capsule after cleaning is carried out drying, and drying, at temperature 26-28 DEG C, is carried out under relative humidity 30-40% condition, static dry.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106923334A (en) * | 2016-12-22 | 2017-07-07 | 云南爱尔康生物技术有限公司 | A kind of astaxanthin flexible glue capsule formula and preparation method thereof |
| CN107912769A (en) * | 2018-01-08 | 2018-04-17 | 王衍斌 | A kind of astaxanthin nutrition replenishers |
| CN108208829A (en) * | 2018-01-08 | 2018-06-29 | 王衍斌 | Astaxanthin nutrition replenishers |
| CN108902958A (en) * | 2018-07-20 | 2018-11-30 | 深圳市博奥生物科技有限公司 | A kind of astaxanthin soft capsule and preparation method thereof |
| CN109481416A (en) * | 2019-01-02 | 2019-03-19 | 陈丽娟 | A kind of astaxanthin soft capsule |
| CN112089058A (en) * | 2020-10-10 | 2020-12-18 | 环贯绿佳利(珠海)科技发展有限公司 | Soft capsule for improving immunity and preparation method thereof |
| CN112167636A (en) * | 2020-06-29 | 2021-01-05 | 武汉林宝莱生物科技有限公司 | Cannabidiol and astaxanthin soft capsule and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6344214B1 (en) * | 1999-12-13 | 2002-02-05 | Cyanotech Corporation | Method for retarding and ameliorating fever blisters and canker sores |
| CN102335420A (en) * | 2011-10-19 | 2012-02-01 | 宁波红龙生物科技有限公司 | Composition containing haematococcus extract and collagen peptide and preparation method for composition |
| CN104523770A (en) * | 2014-12-05 | 2015-04-22 | 黑龙江众生生物工程有限公司 | Auriculacin soft capsule and preparation method thereof |
-
2015
- 2015-09-14 CN CN201510579596.XA patent/CN105055482A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6344214B1 (en) * | 1999-12-13 | 2002-02-05 | Cyanotech Corporation | Method for retarding and ameliorating fever blisters and canker sores |
| CN102335420A (en) * | 2011-10-19 | 2012-02-01 | 宁波红龙生物科技有限公司 | Composition containing haematococcus extract and collagen peptide and preparation method for composition |
| CN104523770A (en) * | 2014-12-05 | 2015-04-22 | 黑龙江众生生物工程有限公司 | Auriculacin soft capsule and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 全日爱商城: "进口虾青素正式获得国家食品药品监督管理局批号", 《HTTP://WWW.QUANRIAI.CN/XQS_ARTICLE-11114.HTML》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106923334A (en) * | 2016-12-22 | 2017-07-07 | 云南爱尔康生物技术有限公司 | A kind of astaxanthin flexible glue capsule formula and preparation method thereof |
| CN107912769A (en) * | 2018-01-08 | 2018-04-17 | 王衍斌 | A kind of astaxanthin nutrition replenishers |
| CN108208829A (en) * | 2018-01-08 | 2018-06-29 | 王衍斌 | Astaxanthin nutrition replenishers |
| CN108902958A (en) * | 2018-07-20 | 2018-11-30 | 深圳市博奥生物科技有限公司 | A kind of astaxanthin soft capsule and preparation method thereof |
| CN109481416A (en) * | 2019-01-02 | 2019-03-19 | 陈丽娟 | A kind of astaxanthin soft capsule |
| CN112167636A (en) * | 2020-06-29 | 2021-01-05 | 武汉林宝莱生物科技有限公司 | Cannabidiol and astaxanthin soft capsule and preparation method thereof |
| CN112089058A (en) * | 2020-10-10 | 2020-12-18 | 环贯绿佳利(珠海)科技发展有限公司 | Soft capsule for improving immunity and preparation method thereof |
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