CN105044297B - A kind of apparatus and method for detecting meat food safety - Google Patents
A kind of apparatus and method for detecting meat food safety Download PDFInfo
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- CN105044297B CN105044297B CN201510303837.8A CN201510303837A CN105044297B CN 105044297 B CN105044297 B CN 105044297B CN 201510303837 A CN201510303837 A CN 201510303837A CN 105044297 B CN105044297 B CN 105044297B
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Abstract
The invention discloses a kind of apparatus and method for detecting meat food safety, including detection case and testing equipment, the testing equipment is located inside the detection case, the detection case includes double-layer box body and the case lid being flexibly connected with it, the testing equipment includes sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, read and data processing equipment and experiment consumptive material, the side of the casing includes wheel body, opposite side corresponding thereto is provided with telescopic lever, the wheel body is that 4 polyurethane aggravate roller, the casing is upper and lower double-layer structure, wherein lower floor is drawer-type structure；Glove cavity is formed respectively inside the Rotating fields up and down and case lid of the casing, wherein it is filled with the supporting mould of novel environment friendly plastics expanded material material, the surface of the supporting mould is provided with the consistent locating slot of same testing equipment shape, the receiving testing equipment is inlayed respectively.
Description
Technical field
It is more particularly to a kind of for detecting meat food safety the present invention relates to a kind of detection field of meat food safety
Devices and methods therefor.
Background technology
Meat is the important foodstuffs in people's diet with the end and accession to WTO of China's shortage economy, its quality and peace
The problems such as full-time benefit is taken seriously that the existing management systems of are unsound, lacks efficient, quick detection means becomes increasingly conspicuous.It is special
It is not the extensive use of agricultural chemicals, veterinary drug, feed, feed addictive, animals and plants hormone etc. in recent years, promoting, agricultural and animal products are big
While amount increases substantially, the hidden danger of agricultural and animal products quality safety is also brought.In addition industrial waste and domestic waste
Pollution to agriculture production environment, residues of pesticides, residue of veterinary drug and the exceeded caused agricultural and animal products of other poisonous and harmful substances are dirty
Dye and meat product poisoning at home and abroad repeatedly occur, and have seriously threatened the healthy and life security of consumer, thus,
The quality and safety of meat product have turned into the focal issue of social extensive concern.
The industry development of China meat product is rapid, but antibiotic, protrudes the problems such as violated veterinary drug is exceeded, seriously restricts China
Animal husbandry development and jeopardize the healthy of consumer.Traditional instrumental method such as chromatography or chromatograph-mass spectrometer coupling technology
Assay method is cumbersome, efficiency is low, is not suitable for the quick detection of a large amount of samples.ELISA method is exempted from using the competitiveness of enzyme marker
Epidemic disease detection method, is widely used as qualitative or half-quantitative detection quick screening method, although contract to a certain extent in recent years
Short detection time, but operating process is still more complicated.More importantly, above detection method is laboratory method, it is difficult to
Apply at the scene, be unfavorable for promoting the use of.At present, the detection to meat product has been not limited solely to laboratory, but extends
To links such as production, cultivation, circulations, and time-bounded requirement is higher, therefore in the urgent need to the complete detection of portability sets
Standby and analysis method, can to a certain extent substitute test in laboratory, reach the effect of primary dcreening operation.
Accordingly, it is desirable to provide a kind of device of field quick detection meat products safety.
The content of the invention
In view of the shortcomings of the prior art, the present invention is provided and can pacified with field quick detection meat product the invention provides one kind
Full device and its method, it is simple structure, low cost, easy to operate.
A kind of device for detecting meat food safety, described device includes detection case and testing equipment, and the detection sets
Standby to be located inside the detection case, the detection case includes double-layer box body and the case lid being flexibly connected with it, the testing equipment
Including sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, reading and data processing equipment and experiment consumptive material, institute
The side for stating casing includes wheel body, and opposite side corresponding thereto is provided with telescopic lever, and the wheel body is that 4 polyurethane aggravate rolling
Wheel, the casing is upper and lower double-layer structure, and wherein lower floor is drawer-type structure；Inside the Rotating fields up and down and case lid of the casing
Glove cavity is formed respectively, wherein the supporting mould filled with novel environment friendly plastics expanded material material, in the table of the supporting mould
Face is provided with the consistent locating slot of same testing equipment shape, and the receiving testing equipment is inlayed respectively.
Preferably, the sample pre-treatments equipment cut including sample treatment, sample treatment knife, marking pen, sample spoon, use
Specification, micro balance, pipettor, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, it is complete from
Control liquid level indicates nitrogen purging instrument；
The collaurum rapid detection card includes that sulfamido detection card, quinolones detection card, chloramphenicol detection card, celebrating are big
Mycin detection card, Clenbuterol detection card, Ractopamine detection card, salbutamol detection card；
The reading and data processing equipment include eight passage collaurum detectors and work computer；
The experiment consumptive material includes emgloves, centrifuge tube, accurate pH test paper, pipette tips, dropper, lab-gown.
Preferably, the detection case is made up of polypropylene composite material, the cell knot of the polypropylene composite material
Structure is mutually twisted to open nucleus between each cell；Multiple dual control locks are additionally provided with the casing or case lid of the detection case.
Preferably, the dual control lock is 7, is uniformly arranged on the edge of the case lid.
Preferably, it is additionally provided with sealing ring at the edge of the case lid；The detection case also includes gas balance valve.
Preferably, it is disposed with by order from left to right, from top to bottom on the supporting mould of the case lid internal cavities
At work computer locating slot, pipettor locating slot, sample treatment knife locating slot, sample spoon locating slot, marking pen locating slot, sample
Reason cuts locating slot, micro balance locating slot and timer locating slot；
On the supporting mould of the box house upper strata cavity colloid is disposed with by order from left to right, from top to bottom
Golden rapid detection card locating slot, vortex centrifugal all-in-one locating slot, eight passage collaurum detector locating slots, Sample Grinder are fixed
Position groove；On the supporting mould of lower floor's drawer-type structure internal cavity full automatic control is disposed with by order from left to right, from top to bottom
Liquid level indicates nitrogen purging instrument locating slot, tissue sample multiplication of voltage rapid treatment instrument locating slot, experiment consumptive material locating slot；
Wherein, the multiple locating slot accommodates corresponding testing equipment, and the operation instructions are put together with work computer
Put in the work computer locating slot.
A kind of detection method using the above-mentioned device for detecting meat food safety, comprises the following steps：
1）Detection card and sample to be checked are recovered to room temperature, collaurum rapid detection card is then taken out
2）Sample extraction instrument is used, is sampled, marking pen mark；Tissue sample weighs 2-5 g in centrifuge tube after crushing, add
Sample extracting solution, is mixed with vortex centrifugal all-in-one, 4000 r/min centrifugation 10-15 min, takes supernatant, to be checked；
The extracting tool is cut including sample treatment, sample treatment knife, sample spoon and micro balance, Sample Grinder；
3）Collaurum rapid detection card is kept flat, the measuring samples solution after being processed with pipettor or dropper absorption, vertically
5-7 drops are added dropwise over to start to use timer timing in well, after sample-adding；
4）Detection card is put into eight passage collaurum detectors after 5 minutes and reads data, data are carried out with notebook computer
Treatment.
Technical scheme has the advantages that：
A kind of devices and methods therefor for detecting meat food safety that the present invention is provided, configures complete, will locate before sample
Reason equipment, detection reagent, detection card, is read and data processing equipment is integrated, it is adaptable to which the scene of meat products safety is fast
Fast examination, can find and monitoring management in time so as to reach to hazardous substance；It is excellent with miniaturization, portable, easy to use etc.
Point；Improve sample pre-treatments efficiency；Dual-use, saves space and cost in casing；The wheel body is that 4 polyurethane aggravate rolling
Wheel, can bear larger weight, compact, conveniently moving, and can be quickly moved to destination carries out Site Detection, with good
Buffering, antidetonation, heat-insulated, moistureproof, resist chemical the advantages of, and it is nontoxic, do not absorb water.
Brief description of the drawings
Below by drawings and Examples, technical scheme is described in further detail.
Fig. 1 is the detection case structural representation in open mode under one embodiment of the present invention;
Fig. 2 is dual control buckle structure schematic diagram of the present invention；
Fig. 3 is the structural representation of the cramp and fixture block of dual control lock of the present invention under opening.
Specific embodiment
In order to have a clear understanding of technical scheme, its detailed structure will be set forth in the description that follows.Obviously, originally
Simultaneously deficiency is limited to the specific details that those skilled in the art is familiar with for the specific execution of inventive embodiments.Preferred reality of the invention
Apply example to be described in detail as follows, except these for describing in detail implement exception, there can also be other embodiment.
The invention will be further described below：
Embodiment 1
As shown in figure 1, a kind of device for detecting meat food safety, including detection case and portion placed within detection
Equipment, the detection case includes casing 2 and the case lid 1 being flexibly connected with it, it is preferred that the casing 2 and case lid 1 are side
It is connected and is flexibly connected, can freely opens and fasten；The casing 2 and case lid 1 can in many ways reach the purpose of fastening, such as
Edge in addition to connecting side sets slide fastener, or sets retaining mechanism mutual cooperation locking.Lower floor is moveable drawer 3, described
Hand grip portion is provided with drawer 3, can freely be taken out and be pushed away, lock is provided between the movable drawer structure 3 of the lower floor of casing 2 and casing 2
Tight mechanism, after pushing lower floor's drawer 3, drawer 3 is stated in fastening lock residence.The retaining mechanism can reach locking mesh for any
Structure, such as can be dual control of the present invention lock.
Testing equipment described in Fig. 1 includes sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, reading sum
According to processing equipment and experiment consumptive material, the internal cavities that the levels and case lid 1 of the casing 2 are formed respectively are filled with novel environment friendly
The supporting mould of plastic foam material material, the consistent positioning of same testing equipment shape is provided with the surface of the supporting mould
Groove, inlays the receiving testing equipment respectively.The sample pre-treatments equipment is cut including sample treatment, sample treatment knife, sample
Spoon, micro balance, pipettor, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, full automatic control liquid
Position indicates nitrogen purging instrument；The collaurum rapid detection card includes sulfamido detection card, quinolones detection card, chloramphenicol detection
Card, gentamicin detection card, Clenbuterol detection card, Ractopamine detection card, salbutamol detection card；The reading sum
Include eight passage collaurum detectors and work computer according to processing equipment；The experiment consumptive material includes emgloves, centrifuge tube, essence
Close pH test paper, pipette tips, dropper, lab-gown.
Preferably, on the supporting mould of the internal cavities of case lid described in Fig. 11 by from left to right, from top to bottom order according to
It is secondary to be provided with work computer locating slot 4, pipettor locating slot 5, sample treatment knife locating slot 6, sample spoon locating slot 7, marking pen
Locating slot 8, sample treatment cuts locating slot 9, micro balance locating slot 10 and timer locating slot 11；In the upper strata of the casing 2
On the supporting mould of portion's cavity by order from left to right, from top to bottom be disposed with collaurum rapid detection card locating slot 12,
Vortex centrifugal all-in-one locating slot 13, eight passage collaurum detector locating slots 14, Sample Grinder locating slot 15；In the case
In lower floor's drawer of the internal cavities of body 2 full Self-controllable level is disposed with supporting mould by order from left to right, from top to bottom
Indicate nitrogen purging instrument locating slot 16, tissue sample multiplication of voltage rapid treatment instrument locating slot 17, experiment consumptive material locating slot 18；Wherein, institute
State multiple locating slots and accommodate corresponding testing equipment, the operation instructions are placed on the work computer with work computer together
In locating slot 4.
Device for detecting meat food safety of the present invention, the side of casing 2 described in wherein Fig. 1 also includes wheel body
（Not shown in figure）, opposite side corresponding thereto is provided with telescopic lever（Not shown in figure）.Compact, conveniently moving can
Site Detection is carried out to be quickly moved to destination.The wheel body is that 4 polyurethane aggravate roller, can bear larger weight, body
Compact, the conveniently moving of product, can be quickly moved to destination carries out Site Detection.The detection case is by polypropylene composite material
It is made, the eucaryotic cell structure of the polypropylene composite material is mutually twisted to open nucleus between each cell, opens nuclear structures
Design, lightweight, intensity is high, firm solid, and another advantage can be effectively to absorb external force, therefore good damping effect.Such as Fig. 2 and
Shown in Fig. 3, multiple dual control locks 20 are additionally provided with the casing 2 or case lid 1 of the detection case, it is preferred that 7 dual control locks
20 edges for being uniformly arranged on the case lid 1.The dual control lock 20 includes the cramp 21 and fixture block 22 that are hinged, the casing 2
Edge be provided with the projection 24 being adapted with the cramp 21, the projection 24 is arranged on the one of the top edge of the casing 2
Circle；The cramp 21 and the projection 24 are fastened closes the detection case, closes the backward direction of the casing 2 and promotes the fixture block
22, fastening is more closed, the cramp 21 and fixture block 22 are in the on state as shown in figure 3, such as Fig. 2 institutes under buckling state
Show, can be opened in the case where having soil, having sand, shut, traditional design is difficult to shut as latched into dust；In dual control lock
The through hole 23 passed through for lockset is also respectively provided with the side of button 20 and casing 2 corresponding thereto, lockset leads to through described
Hole 23 is locked by the casing 2 and case lid 1.Sealing ring is additionally provided with the edge of the case lid 1, obturation effect is good；The inspection
Measuring tank also includes gas balance valve 19, adjusts inside and outside differential pressure, and air can enter under plateau, high altitude environment, and hydrone can not enter
Go, it is moistureproof.
Device for detecting meat food safety of the present invention antibiotic in quick detection meat products, violated at the scene
The exceeded application of veterinary drug.
Embodiment 2
A kind of method that complete set of equipments using described field quick detection meat products safety is detected, including it is as follows
Step：
（1）It is first complete before being tested to read operation instructions, and detection card and sample to be checked are recovered to room temperature；From
Collaurum rapid detection card is taken out in original packing bag；
（2）Sample extraction instrument is used, is sampled, marking pen mark；Tissue sample weighs 1-5 g in centrifuge tube after crushing, plus
Enter sample extracting solution, mixed with vortex centrifugal all-in-one, 4000 r/min are centrifuged 10-15min, take supernatant, to be checked；
The extracting tool is cut including sample treatment, sample treatment knife, sample spoon and micro balance, Sample Grinder；
（3）Collaurum rapid detection card is kept flat, the measuring samples solution after being processed with pipettor or dropper absorption, vertically
5-7 drops are added dropwise over to start to use timer timing in well, after sample-adding；
（4）Detection card is put into eight passage collaurum detectors after 5 minutes and reads data, data are carried out with notebook computer
Treatment.
Embodiment 3
A kind of method for detecting meat food safety, comprises the following steps：
（1）It is first complete before being tested to read operation instructions, and detection card and sample to be checked are recovered to room temperature；From
Clenobuterol hydrochloride detection card is taken out in original packing bag；
（2）Fresh pork 2-5 g are weighed with micro balance, is put into tissue sample multiplication of voltage rapid treatment instrument and is squeezed out sample
Liquid, 10-15 min are centrifuged with the r/min of vortex centrifugal all-in-one 4000, take supernatant, to be checked；
（3）Clenobuterol hydrochloride detection card is kept flat, the measuring samples after being processed with pipettor or dropper absorption are molten
Liquid, is vertically added dropwise over 5-7 drops in well, and timing is started after sample-adding；
（4）Detection card is put into eight passage collaurum detectors after 5 minutes and reads data, data are carried out with notebook computer
Treatment.
Embodiment 4
A kind of its method for detecting meat food safety, comprises the following steps：
（1）It is first complete before being tested to read operation instructions, and detection card and sample to be checked are recovered to room temperature；From
Chloramphenicol detection card is taken out in original packing bag；
（2）Sample extraction instrument is used, is sampled, marking pen mark weighs the pork sample after 2-5 g are crushed, and adds sample
Extract solution, is mixed with vortex centrifugal all-in-one, 4000 r/min centrifugations 10-15min；The mL of supernatant 1 is taken in 10 mL centrifuge tubes
In, indicate 50 DEG C or so of nitrogen blow device to be evaporated using full Self-controllable level；Add 500 μ L redissolve liquid, vortex oscillation, and with drip
Continuous drawing wash is managed, until being completely dissolved tube wall residual；It is to be checked.
（3）Chloramphenicol detection card is kept flat, the measuring samples solution after being processed with pipettor or dropper absorption, vertically
5-7 drops are added dropwise in well, timing is started after sample-adding；
（4）Detection card is put into eight passage collaurum detectors after 5 minutes and reads data, data are carried out with notebook computer
Treatment.
Finally it should be noted that：The above embodiments are merely illustrative of the technical solutions of the present invention rather than its limitations, to the greatest extent
Pipe has been described in detail with reference to above-described embodiment to the present invention, and those of ordinary skill in the art still can be to this hair
Bright specific embodiment is modified or equivalent, these without departing from spirit and scope of the invention any modification or
Equivalent, is applying within pending claims.
Claims (5)
1. a kind of device for detecting meat food safety, it is characterised in that described device includes detection case and testing equipment, institute
Testing equipment is stated inside the detection case, the detection case includes double-layer box body and the case lid being flexibly connected with it, described
Testing equipment includes sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, reading and data processing equipment and experiment
Consumptive material, the side of the casing includes wheel body, and opposite side corresponding thereto is provided with telescopic lever, and the wheel body is 4 poly- ammonia
Ester aggravates roller, and the casing is upper and lower double-layer structure, and wherein lower floor is drawer-type structure；The Rotating fields up and down of the casing and
Glove cavity is formed inside case lid respectively, wherein the supporting mould filled with novel environment friendly plastics expanded material material, in the branch
The surface for protecting mould is provided with the consistent locating slot of same testing equipment shape, and the receiving testing equipment, the sample are inlayed respectively
Product pre-processing device is cut including sample treatment, sample treatment knife, marking pen, sample spoon, operation instructions, micro balance, liquid relief
Device, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, full Self-controllable level indicate nitrogen purging instrument；
The collaurum rapid detection card includes sulfamido detection card, quinolones detection card, chloramphenicol detection card, gentamicin
Detection card, Clenbuterol detection card, Ractopamine detection card, salbutamol detection card；
The reading and data processing equipment include eight passage collaurum detectors and work computer；
The experiment consumptive material includes emgloves, centrifuge tube, accurate pH test paper, pipette tips, dropper, lab-gown, inside the case lid
On the supporting mould of cavity by order from left to right, from top to bottom be disposed with work computer locating slot, pipettor locating slot,
Sample treatment knife locating slot, sample spoon locating slot, marking pen locating slot, sample treatment cut locating slot, micro balance locating slot and
Timer locating slot；
It is fast by order from left to right, from top to bottom collaurum to be disposed with the supporting mould of box house upper strata cavity
Speed detection card locating slot, vortex centrifugal all-in-one locating slot, eight passage collaurum detector locating slots, Sample Grinder positioning
Groove；On the supporting mould of lower floor's drawer-type structure internal cavity full automatic control liquid is disposed with by order from left to right, from top to bottom
Position indicates nitrogen purging instrument locating slot, tissue sample multiplication of voltage rapid treatment instrument locating slot, experiment consumptive material locating slot；
Wherein, the multiple locating slot accommodates corresponding testing equipment, and the operation instructions are placed on together with work computer
In the work computer locating slot.
2. the device for detecting meat food safety according to claim 1, it is characterised in that the detection case is by poly- third
Alkene composite materials are made, and the eucaryotic cell structure of the polypropylene composite material is mutually twisted to open nucleus between each cell；
Multiple dual control locks are additionally provided with the casing or case lid of the detection case.
3. the device for detecting meat food safety according to claim 2, it is characterised in that the dual control lock is 7
It is individual, it is uniformly arranged on the edge of the case lid.
4. the device for detecting meat food safety according to claim 3, it is characterised in that at the edge of the case lid
It is additionally provided with sealing ring；The detection case also includes gas balance valve.
5. the detection of the device for detecting meat food safety in a kind of use claim 1-4 described in any one claim
Method, it is characterised in that comprise the following steps：
1) detection card and sample to be checked are recovered to room temperature, then takes out collaurum rapid detection card
2) sample extraction instrument is used, is sampled, marking pen mark；Tissue sample weighs 2-5g in centrifuge tube after crushing, add sample
Extract solution, is mixed with vortex centrifugal all-in-one, 4000r/min centrifugation 10-15min, takes supernatant, to be checked；
The extracting tool is cut including sample treatment, sample treatment knife, sample spoon and micro balance, Sample Grinder；
3) collaurum rapid detection card is kept flat, the measuring samples solution after being processed with pipettor or dropper absorption, vertically dropwise
5-7 drops are added to start to use timer timing in well, after sample-adding；
4) detection card is put into eight passage collaurum detectors after 5 minutes and reads data, data processing is carried out with notebook computer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510303837.8A CN105044297B (en) | 2015-06-05 | 2015-06-05 | A kind of apparatus and method for detecting meat food safety |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510303837.8A CN105044297B (en) | 2015-06-05 | 2015-06-05 | A kind of apparatus and method for detecting meat food safety |
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| Publication Number | Publication Date |
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| CN105044297A CN105044297A (en) | 2015-11-11 |
| CN105044297B true CN105044297B (en) | 2017-05-31 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108982143A (en) * | 2018-06-25 | 2018-12-11 | 杭州富阳福士得食品有限公司 | A kind of food inspection extraction element with leakproofness |
| CN115356484B (en) * | 2022-10-20 | 2023-01-24 | 黑龙江省农业科学院农产品质量安全研究所 | Complete equipment and detection method for rapidly detecting mycotoxin in grains |
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| CN105044297A (en) | 2015-11-11 |
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