CN105039315A - Plant bleeding sap RNA extracting method - Google Patents
Plant bleeding sap RNA extracting method Download PDFInfo
- Publication number
- CN105039315A CN105039315A CN201510589286.6A CN201510589286A CN105039315A CN 105039315 A CN105039315 A CN 105039315A CN 201510589286 A CN201510589286 A CN 201510589286A CN 105039315 A CN105039315 A CN 105039315A
- Authority
- CN
- China
- Prior art keywords
- supernatant
- add
- 13000rpm
- water
- bleeding sap
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000740 bleeding effect Effects 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title abstract description 8
- 239000006228 supernatant Substances 0.000 claims abstract description 58
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 46
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 33
- 150000002989 phenols Chemical class 0.000 claims abstract description 23
- 238000001556 precipitation Methods 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims abstract description 10
- 238000002123 RNA extraction Methods 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 10
- 229920006395 saturated elastomer Polymers 0.000 claims description 9
- 230000015556 catabolic process Effects 0.000 abstract description 2
- 238000006731 degradation reaction Methods 0.000 abstract description 2
- 238000005119 centrifugation Methods 0.000 abstract 6
- 235000019441 ethanol Nutrition 0.000 abstract 2
- 238000003756 stirring Methods 0.000 abstract 2
- 238000007605 air drying Methods 0.000 abstract 1
- 238000003287 bathing Methods 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 15
- 229960004756 ethanol Drugs 0.000 description 10
- 238000001502 gel electrophoresis Methods 0.000 description 9
- 238000010839 reverse transcription Methods 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 6
- 239000000284 extract Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- -1 phenolic acid (water-saturated phenol Chemical class 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
Landscapes
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a plant bleeding sap RNA extracting method. The plant bleeding sap RNA extracting method comprises the following steps that freeze frying is conducted on plant bleeding sap, and SDS is added to perform water bathing; water saturated phenol is added and stirred, and then chloroform is added and stirred evenly for centrifugation; supernatant is taken into a novel centrifugation tube and added with water saturated phenol, even stirring is performed, then chloroform is added and stirred evenly for centrifugation; supernatant is taken and added with chloroform, even stirring is performed for centrifugation, and supernatant is taken and added with the same volume of isopropanol for standing; centrifugation is performed, the supernatant is poured off, 80% of ethyl alcohol is adopted for washing and precipitation, 100% of ethyl alcohol is adopted for rewashing, centrifugation and air drying are performed for RNA precipitation, and DEPC water is added. The plant bleeding sap RNA extracting method can inhibit degradation of the RNA in bleeding sap and improve the extracting efficiency of the RNA in bleeding sap. RNA extracting procedures are simplified, the obtained RNA amount is increased, and the amount of RNA extracted from the bleeding sap can meet the inverse transcription demand.
Description
Technical field
The present invention relates to agricultural technology field, be specifically related to a kind of plant bleeding sap RNA extraction method.
Background technology
At present, extract plant tissue RNA method very ripe, but applying these methods extracts RNA in the plant such as grape, cucumber bleeding sap, effect is undesirable, occurs problem: 1) can not carry RNA; 2) in bleeding sap, RNA degraded is fast; 3) the RNA quantity not sufficient proposed, can not meet the needs of reverse transcription.
Summary of the invention
Goal of the invention: the object of this invention is to provide a kind of plant bleeding sap RNA extraction method.
Technical scheme: in order to solve the problems of the technologies described above, the invention provides a kind of plant bleeding sap RNA extraction method, comprises the following steps:
1) by the lyophilize of plant bleeding sap to the 1/8-1/10 of original volume, add the SDS of 1300-1500 μ l, water-bath 65 DEG C of 25 ~ 30min;
2) add the water-saturated phenol of 200-400 μ l, vortex, then add 200-400 μ l chloroform, vortex mixes, 4 DEG C, the centrifugal 8 ~ 10min of 12000 ~ 13000rpm;
3) get 1100-1300 μ l supernatant liquor in new centrifuge tube, add the water-saturated phenol of 1/3 volume, vortex mixes, then adds the chloroform of supernatant liquor 1/3 volume, and vortex mixes, the centrifugal 8 ~ 10min of 12000 ~ 13000rpm;
4) get the water-saturated phenol that supernatant 900-1100 μ l adds supernatant liquor 1/2 volume, vortex mixes, then adds the chloroform vortex mixing of supernatant liquor 1/2 volume, the centrifugal 8 ~ 10min of 12000 ~ 13000rpm;
5) get supernatant 700-900 μ l and add equal-volume chloroform, vortex mixes, and the centrifugal 10min of 12000 ~ 13000rpm, gets supernatant 600-800 μ l and add equal-volume Virahol ,-20 DEG C of standing more than 5h;
6) the centrifugal 20min of 12000 ~ 13000rpm, outwells supernatant, and 80% ethanol 1ml washes precipitation, 12000 ~ 13000rpm, 5 ~ 8min, and 100% ethanol is washed once again, the centrifugal 5min of 12000 ~ 13000rpm, dries RNA precipitation, adds the DEPC water of 30 μ l.
Wherein, as preferably, the plant bleeding sap lyophilize in step 1), to 1/10 of original volume, adds the SDS of 1400 μ l, water-bath 65 DEG C of 30min.
Wherein, as preferably, step 2) in add 300 μ l water-saturated phenols, vortex, then add 300ul chloroform, vortex mixes, 4 DEG C, the centrifugal 10min of 13000rpm.
Wherein, as preferably, get 1200 μ l supernatants in new centrifuge tube, add 400 μ l water-saturated phenols in step 3), vortex mixes, then adds 400 μ l chloroforms, and vortex mixes, the centrifugal 10min of 13000rmp.
Wherein, as preferably, step 4) is got supernatant 1000 μ l and is added 500 μ l water-saturated phenols, and vortex mixes, then adds the mixing of 500ul chloroform vortex, the centrifugal 10min of 13000rpm.
Wherein, as preferably, step 5) is got supernatant 800 μ l and is added 800 μ l chloroforms, and vortex mixes, and the centrifugal 10min of 13000rpm, gets supernatant 700 μ l and add equal-volume Virahol ,-20 DEG C of standing more than 5h.
Wherein, as preferably, the centrifugal 20min of step 6) 13000rpm, outwells supernatant, and 80% ethanol 1ml washes precipitation, 13000rpm, 5min, and 100% ethanol is washed once again, the centrifugal 5min of 13000rpm, dries RNA precipitation, adds 30 μ lDEPC water.
Beneficial effect: plant bleeding sap RNA extraction method of the present invention can suppress RNA degraded in bleeding sap, improve RNA extraction efficiency in bleeding sap, this invention simplifies RNA extraction procedure, improve RNA and obtain quantity, bleeding sap RNA extracted amount can meet reverse transcription needs.
Accompanying drawing explanation
The RNA detected through gel electrophoresis figure that Fig. 1 embodiment of the present invention 1 ~ 3 is extracted;
After the RNA reverse transcription that Fig. 2 embodiment of the present invention 1 ~ 3 is extracted, cDNA detected through gel electrophoresis figure;
The RNA detected through gel electrophoresis figure that Fig. 3 comparative example is extracted;
After the RNA reverse transcription that Fig. 4 comparative example is extracted, cDNA detected through gel electrophoresis figure.
Embodiment
Below technical solution of the present invention is described in detail, but protection scope of the present invention is not limited to described embodiment.
embodiment 1:
One kind of plant bleeding sap RNA extraction method, comprises the following steps:
1) by the lyophilize of 5ml plant bleeding sap to 1/10 of original volume, add 1400 μ lSDS, water-bath 65 DEG C of 30min;
2) add 300 μ l water-saturated phenols, vortex, then add 300 μ l chloroforms, vortex mixes, 4 DEG C, the centrifugal 10min of 13000rpm;
3) get 1200 μ l supernatants in new centrifuge tube, add 400 μ l water-saturated phenols, vortex mixes, then adds 400 μ l chloroforms, and vortex mixes, the centrifugal 10min of 13000rmp;
4) get supernatant 1000 μ l and add 500 μ l water-saturated phenols, vortex mixes, then adds 500 μ l chloroform vortex mixings, the centrifugal 10min of 13000rpm;
5) get supernatant 800 μ l and add 800 μ l chloroforms, vortex mixes, and the centrifugal 10min of 13000rpm, gets supernatant 700 μ l and add equal-volume Virahol ,-20 DEG C of standing more than 5h;
6) the centrifugal 20min of 13000rpm, outwells supernatant, and 80% ethanol 1ml washes precipitation, 13000rpm, 5min, and 100% ethanol is washed once again, the centrifugal 5min of 13000rpm, dries RNA precipitation, adds 30 μ lDEPC water.
Embodiment 2
One kind of plant bleeding sap RNA extraction method, comprises the following steps:
1) by the lyophilize of plant bleeding sap to 1/8 of original volume, add the SDS of 1300 μ l, water-bath 65 DEG C of 25min;
2) add the water-saturated phenol of 200 μ l, vortex, then add 200 μ l chloroforms, vortex mixes, 4 DEG C, the centrifugal 8min of 12000rpm;
3) get 1100 μ l supernatant liquors in new centrifuge tube, add the water-saturated phenol of 1/3 volume, vortex mixes, then adds the chloroform of supernatant liquor 1/3 volume, and vortex mixes, the centrifugal 8min of 12000rpm;
4) get the water-saturated phenol that supernatant 900 μ l adds supernatant liquor 1/2 volume, vortex mixes, then adds the chloroform vortex mixing of supernatant liquor 1/2 volume, the centrifugal 8min of 12000rpm;
5) get supernatant 700 μ l and add equal-volume chloroform, vortex mixes, and the centrifugal 10min of 12000rpm, gets supernatant 600 μ l and add equal-volume Virahol ,-20 DEG C of standing more than 5h;
6) the centrifugal 20min of 12000rpm, outwells supernatant, and 80% ethanol 1ml washes precipitation, 12000rpm, 8min, and 100% ethanol is washed once again, the centrifugal 5min of 12000rpm, dries RNA precipitation, adds the DEPC water of 30 μ l.
Embodiment 3
One kind of plant bleeding sap RNA extraction method, comprises the following steps:
1) by the lyophilize of plant bleeding sap to 1/9 of original volume, add the SDS of 1500 μ l, water-bath 65 DEG C of 30min;
2) add the water-saturated phenol of 400 μ l, vortex, then add 400 μ l chloroforms, vortex mixes, 4 DEG C, the centrifugal 9min of 12500rpm;
3) get 1300 μ l supernatant liquors in new centrifuge tube, add the water-saturated phenol of 1/3 volume, vortex mixes, then adds the chloroform of supernatant liquor 1/3 volume, and vortex mixes, the centrifugal 9min of 12500rpm;
4) get the water-saturated phenol that supernatant 1100 μ l adds supernatant liquor 1/2 volume, vortex mixes, then adds the chloroform vortex mixing of supernatant liquor 1/2 volume, the centrifugal 9min of 12500rpm;
5) get supernatant 900 μ l and add equal-volume chloroform, vortex mixes, and the centrifugal 10min of 12500rpm, gets supernatant 800 μ l and add equal-volume Virahol ,-20 DEG C of standing more than 5h;
6) the centrifugal 20min of 12500rpm, outwells supernatant, and 80% ethanol 1ml washes precipitation, 12500rpm, 7min, and 100% ethanol is washed once again, the centrifugal 5min of 12500rpm, dries RNA precipitation, adds the DEPC water of 30 μ l.
The present embodiment obtains the amount of RNA between 1.8-2.2 μ g/ μ l, obtains the success ratio that can meet reverse transcription RNA concentration requirement and reaches more than 99%, RNA degradation rate and be less than 0.5%.
Comparative example:
1. water-bath is adjusted to 65 DEG C;
2. in 2ml centrifuge tube, add 1100 μ l lysate SDS, then add 50 μ l beta-mercaptoethanol mixings, or in 2ml centrifuge tube, add 1100 μ l lysate CTAB, then add 30 μ l beta-mercaptoethanol CTAB;
3. grind away, is added to centrifuge tube, and timing puts 30 ~ 45min in water-bath, its interval 10min transpose;
4. add chloroform about 850 μ l after taking out cooling, vortex mixes, 12000r/m, centrifugal 20min;
5. in new 2ml centrifuge tube, add 450 μ l phenolic acid (water-saturated phenol, lower floor is phenol), with supernatant liquor (1000 μ l) vortex, 12000r/m, centrifugal (18min ~ 20min);
6. in new 2ml centrifuge tube, add 900 μ l chloroforms, add supernatant liquid (about 850 μ l) vortex, 12000r/m, 18min(15 ~ 20min);
7. in new 1.5ml centrifuge tube, add 35 μ lNaAC and 70 μ l dehydrated alcohols, get supernatant liquid about 700 μ l and add wherein, put upside down mixing, place on water box ,-20 DEG C of standing more than 60min();
The centrifugal 20min of 8.12000r/m, gets supernatant liquid about 700 μ l and adds equal-volume Virahol and put upside down mixing ,-20 DEG C, crosses liquid precipitate (more than 5h);
The centrifugal 20min of 12000r/m, outwells supernatant liquid, adds 750 μ l dehydrated alcohol+250 μ lDEPC water to precipitation, flick → 12000r/m, 6min abandons supernatant, is placed in 750 μ l dehydrated alcohols that paper slightly absorbs water → add, 12000r/m, 6min → abandon supernatant, on paper dry (about more than 10min), add 45 μ lDEPC water, dissolution precipitation, put into 65 DEG C of water-bath 10min, then-20 DEG C of preservations.
The embodiment of the present invention 1 ~ 3 extracts bleeding sap RNA see Fig. 1: RNA detected through gel electrophoresis of carrying (A, B, C repeat for tri-times to extract) gel electrophoresis images band is clear, and brightness is high, and RNA extracted amount foot is described; Fig. 2: extract RNA reverse transcription after, cDNA detected through gel electrophoresis: cDNA band is clear, illustrates that reverse transcription is effective.
Comparative example extracts bleeding sap RNA see Fig. 3: bleeding sap RNA(A, B, C repeat for tri-times to extract) gel electrophoresis images band is fuzzy, low lightness, and RNA extracted amount deficiency is described; After Fig. 4 extracts RNA reverse transcription, cDNA detected through gel electrophoresis: have no cDNA band, illustrates that the amount of RNA extraction from bleeding sap does not reach the requirement of reverse transcription.
Claims (7)
1. a kind of plant bleeding sap RNA extraction method, is characterized in that: comprise the following steps:
1) by the lyophilize of plant bleeding sap to the 1/8-1/10 of original volume, add the SDS of 1300-1500 μ l, water-bath 65 DEG C of 25 ~ 30min;
2) add the water-saturated phenol of 200-400 μ l, vortex, then add 200-400 μ l chloroform, vortex mixes, 4 DEG C, the centrifugal 8 ~ 10min of 12000 ~ 13000rpm;
3) get 1100-1300 μ l supernatant liquor in new centrifuge tube, add the water-saturated phenol of 1/3 volume, vortex mixes, then adds the chloroform of supernatant liquor 1/3 volume, and vortex mixes, the centrifugal 8 ~ 10min of 12000 ~ 13000rpm;
4) get the water-saturated phenol that supernatant 900-1100 μ l adds supernatant liquor 1/2 volume, vortex mixes, then adds the chloroform vortex mixing of supernatant liquor 1/2 volume, the centrifugal 8 ~ 10min of 12000 ~ 13000rpm;
5) get supernatant 700-900 μ l and add equal-volume chloroform, vortex mixes, and the centrifugal 10min of 12000 ~ 13000rpm, gets supernatant 600-800 μ l and add equal-volume Virahol ,-20 DEG C of standing more than 5h;
6) the centrifugal 20min of 12000 ~ 13000rpm, outwells supernatant, and 80% ethanol 1ml washes precipitation, 12000 ~ 13000rpm, 5 ~ 8min, and 100% ethanol is washed once again, the centrifugal 5min of 12000 ~ 13000rpm, dries RNA precipitation, adds the DEPC water of 30 μ l.
2. a kind of plant bleeding sap RNA extraction method according to claim 1, is characterized in that: the plant bleeding sap lyophilize in described step 1), to 1/10 of original volume, adds the SDS of 1400 μ l, water-bath 65 DEG C of 30min.
3. a kind of plant bleeding sap RNA extraction method according to claim 1, is characterized in that: described step 2) in add 300 μ l water-saturated phenols, vortex, then add 300ul chloroform, vortex mixes, 4 DEG C, the centrifugal 10min of 13000rpm.
4. a kind of plant bleeding sap RNA extraction method according to claim 1, is characterized in that: get 1200 μ l supernatants in described step 3) in new centrifuge tube, add 400 μ l water-saturated phenols, vortex mixes, add 400 μ l chloroforms again, vortex mixes, the centrifugal 10min of 13000rmp.
5. a kind of plant bleeding sap RNA extraction method according to claim 1, is characterized in that: described step 4) is got supernatant 1000 μ l and added 500 μ l water-saturated phenols, and vortex mixes, then adds the mixing of 500ul chloroform vortex, the centrifugal 10min of 13000rpm.
6. a kind of plant bleeding sap RNA extraction method according to claim 1, it is characterized in that: described step 5) is got supernatant 800 μ l and added 800 μ l chloroforms, vortex mixes, the centrifugal 10min of 13000rpm, get supernatant 700 μ l and add equal-volume Virahol ,-20 DEG C of standing more than 5h.
7. a kind of plant bleeding sap RNA extraction method according to claim 1, it is characterized in that: the centrifugal 20min of described step 6) 13000rpm, outwell supernatant, 80% ethanol 1ml washes precipitation, 13000rpm, 5min, 100% ethanol is washed once again, the centrifugal 5min of 13000rpm, dries RNA precipitation, adds 30 μ lDEPC water.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510589286.6A CN105039315A (en) | 2015-09-16 | 2015-09-16 | Plant bleeding sap RNA extracting method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510589286.6A CN105039315A (en) | 2015-09-16 | 2015-09-16 | Plant bleeding sap RNA extracting method |
Publications (1)
Publication Number | Publication Date |
---|---|
CN105039315A true CN105039315A (en) | 2015-11-11 |
Family
ID=54446288
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510589286.6A Pending CN105039315A (en) | 2015-09-16 | 2015-09-16 | Plant bleeding sap RNA extracting method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105039315A (en) |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102283408A (en) * | 2011-06-17 | 2011-12-21 | 合肥红峥生物科技有限公司 | Loofah bleeding sap functional beverage and preparation method thereof |
CN102628039A (en) * | 2012-04-11 | 2012-08-08 | 中国林业科学研究院林业研究所 | General plant total RNA (Ribose Nucleic Acid) extraction method |
CN102871196A (en) * | 2011-07-15 | 2013-01-16 | 伊力哈木·托合迪 | Grape wound liquid drink containing zinc metallothionein (Zn-MT) and preparation method thereof |
CN102884191A (en) * | 2010-02-26 | 2013-01-16 | 凯杰有限公司 | Process for parallel isolation and/or purification of RNA and DNA |
CN103083216A (en) * | 2011-11-01 | 2013-05-08 | 杨秀梅 | All-natural cucumber vine bleeding sap beautifying water |
CN103911369A (en) * | 2014-04-04 | 2014-07-09 | 中国农业科学院烟草研究所 | Method of effectively extracting total RNA (Ribonucleic Acid) of tobacco mature leaf |
CN104313014A (en) * | 2014-10-08 | 2015-01-28 | 福建农林大学 | Method for rapidly extracting total ribonucleic acid (RNA) of fir |
CN104357439A (en) * | 2014-11-27 | 2015-02-18 | 广东省农业科学院作物研究所 | Method for extracting RNA (ribonucleic acid) from plant material containing rich polysaccharides and polyphenols |
-
2015
- 2015-09-16 CN CN201510589286.6A patent/CN105039315A/en active Pending
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102884191A (en) * | 2010-02-26 | 2013-01-16 | 凯杰有限公司 | Process for parallel isolation and/or purification of RNA and DNA |
CN102283408A (en) * | 2011-06-17 | 2011-12-21 | 合肥红峥生物科技有限公司 | Loofah bleeding sap functional beverage and preparation method thereof |
CN102871196A (en) * | 2011-07-15 | 2013-01-16 | 伊力哈木·托合迪 | Grape wound liquid drink containing zinc metallothionein (Zn-MT) and preparation method thereof |
CN103083216A (en) * | 2011-11-01 | 2013-05-08 | 杨秀梅 | All-natural cucumber vine bleeding sap beautifying water |
CN102628039A (en) * | 2012-04-11 | 2012-08-08 | 中国林业科学研究院林业研究所 | General plant total RNA (Ribose Nucleic Acid) extraction method |
CN103911369A (en) * | 2014-04-04 | 2014-07-09 | 中国农业科学院烟草研究所 | Method of effectively extracting total RNA (Ribonucleic Acid) of tobacco mature leaf |
CN104313014A (en) * | 2014-10-08 | 2015-01-28 | 福建农林大学 | Method for rapidly extracting total ribonucleic acid (RNA) of fir |
CN104357439A (en) * | 2014-11-27 | 2015-02-18 | 广东省农业科学院作物研究所 | Method for extracting RNA (ribonucleic acid) from plant material containing rich polysaccharides and polyphenols |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101032322B (en) | Phyllanthus emblica extract and its preparation and application | |
CN104441155B (en) | A kind of rattan processing method | |
CN104626309A (en) | Bamboo softening and modifying technology | |
CN108078881A (en) | A kind of skin repair liquid | |
CN106720511A (en) | A kind of preparation method of Pasania cuspidata black tea | |
CN103497838B (en) | Method for extracting peony essential oil from peonies | |
CN104256635B (en) | A kind of method of pressure ultrasonic wave added ethonal extraction apple polyphenol | |
CN105039315A (en) | Plant bleeding sap RNA extracting method | |
CN106860148B (en) | A kind of striae of pregnancy repair cream and preparation method thereof | |
CN104398441A (en) | Seaweed pigment hair dye and application method thereof | |
CN104788526B (en) | A kind of sapindoside liquid containing clear stable compound and preparation method thereof | |
CN106806176A (en) | Tea bran extract and preparation method thereof | |
CN108142590A (en) | A kind of black tea processing method | |
CN104327131B (en) | Method for extracting punicalagin from pomegranate peel | |
CN109984989A (en) | A kind of Chinese medicine compound prescription hair dye and preparation method thereof and application method | |
CN111217696A (en) | Method for extracting gallic acid from rhus chinensis roots | |
CN104286151A (en) | Preservative for pears | |
CN104593156A (en) | Method for extracting chimonanthus salicifolius essential oil | |
CN107536768A (en) | A kind of adlay whitening sun protection facial treatment milk and preparation method thereof | |
CN108835635A (en) | A method of antioxidant is extracted by grape pip | |
CN106397165A (en) | Method for preparing cinnamaldehyde | |
CN105496885A (en) | Cosmetic containing plant elements and production method thereof | |
CN104435061A (en) | Method for extracting persimmon polyphenol from persimmons | |
CN105232803A (en) | Method for producing grape seed extract with low pesticide residues and high (-)-epicatechin 3-O-gallate ester content | |
CN110237000A (en) | A kind of Chinese medicine compound prescription hair dye and preparation method thereof and application method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151111 |