CN105028485B - Regulation and control growth-promoting agent and the application process in crop field tea tree sprouting is expedited the emergence of in a kind of tea tree - Google Patents

Abstract

The invention proposes a preparation and application method of a tea tree internal regulation and growth promoter. The method utilizes the biological characteristics of the endophytic spirulina of the tea tree, ferments it to prepare the culture solution of the endophytic spirulina of the tea tree, and sprays the pruned tea tree branches after dilution according to a certain ratio. The bacteria in the growth promoter enter the tea tree branches to symbiose and secrete metabolic active substances to promote the rapid growth of new buds of the tea tree. The new shoots of the treated tea tree grow vigorously, the leaves are dark green, and the length and weight of the new shoots increase significantly. The technical method of the invention has the advantages of low cost, simple operation, green safety, multiple efficacy and long-lasting effect.

Description

A kind of growth-promoting agent for internal regulation and control of tea trees and its application method in promoting the growth of new buds of field tea trees

technical field

The invention relates to a growth-promoting agent for internal regulation of new shoots of plants, in particular to a method for promoting the growth of side buds of new shoots of field tea trees by using endophytic spirulina.

Background technique

Tea tree is an important economic crop in my country, and it is cultivated in various provinces in southern my country. After the tea leaves are picked, they can be made into green tea, white tea, yellow tea, black tea and black tea through a certain process, and the tea branches can be made into stem tea. In addition to being used as a drink, the active ingredients of tea can also be used in medicine, food, chemical industry, etc. Tea tree is a perennial economic crop whose main object is to harvest young shoots and leaves. New green and nutritious young shoots can be picked from tea trees many times a year. According to the season of picking, there are usually spring tea, summer tea and autumn tea. Picking multiple times will inevitably consume a lot of nutrients from the tea tree. How to maintain the luxuriance of the tree body and continue to expand and re-grow, to meet the healthy growth of tea trees, to make them high-quality, stable and high-yielding is an important task faced by tea garden field management. In order to increase the yield of tea, in addition to daily management, it is mainly through rational fertilization to meet the nutritional needs of tea trees. In addition, plant hormones such as gibberellin, triacontanol, sodium nitrophenolate, 6-benzylaminopurine, brassinolide, 4-iodophenoxyacetic acid (Zengchanling) and other plant hormones are used to promote the growth of tea buds to improve tea production. Although artificially synthesized plant hormones have the advantages of low cost and fast growth-promoting effect, they have the disadvantages of single effect, short-lasting effect, strict requirements for concentration, soil residue risk, and failure to meet the requirements of green ecology and environmental protection. In addition, most of the synthetic plant hormones stimulate the division and growth of plant cells. If the nutrition of the tea tree has not kept up, repeated use of artificially synthesized plant hormones may affect the accumulation of nutrients in the new tea cells.

Endophytic spirulina of tea tree is an endophytic bacterium with strong symbiotic specificity in tea tree. It colonizes the tea tree through the wound, regulates and promotes the growth of the tea tree. The literature "Microbiological characteristics of two strains of endogenous Helicospirosis in tea trees" (Wang Ting et al., Journal of Microbiology 2014 No. 4, 2014.04.04, P424-432) described in detail the screening and microbiological characteristics of endogenous Helicospirosis in tea trees , but did not involve the application of endophytic Helicospirilla in tea trees.

Contents of the invention

The purpose of the present invention is to propose a preparation and application method of a tea tree internal regulation and growth promoter. Treating the cut tea tree branches with the growth-promoting agent can effectively promote the growth of the side buds of the tea tree branches and increase the picking amount of tea leaves.

The present invention is achieved like this. The steps are

1. Preparation of growth-promoting agent in tea tree regulation

Take the endophytic Helicospirilla strains of tea trees stored at -80°C, inoculate 5 mL of nutrient broth medium (10 g tryptone, 3 g beef powder, 5 g NaCl, 10 μg/mL spectinomycin, 5 μg/mL ampicillin, add water to 1000 ml ), shake at 150 rpm, and incubate overnight at 37°C. Inject the activated bacterial solution into fresh LB liquid medium (10g tryptone, 5g yeast extract, 10g NaCl, 10μg/mL spectinomycin, 5μg/mL ampicillin at a ratio of 1:100, add water to 1000ml, pH7 .2-7.4), the culture conditions are as follows: the rotating speed of the shaker is 200 revolutions per minute, and the temperature is 28-37°C. After the bacteria have been cultured for 6-8 hours and the OD ₆₀₀ value is 1.0-1.2, all the bacterial culture solution is collected, added with 1% glycerol and stored at 4°C for later use or used directly.

2. Preparation of working solution

When in use, dilute the amplified cultured tea tree endospira spirulina bacteria solution with water at a ratio of 0.5-2:1 to obtain the working solution.

The above-mentioned diluted bacterial culture solution is the growth-promoting agent for internal regulation of tea trees of the present invention.

The application of described accelerant:

1. Treatment of tea tree branches: According to the conventional tea tree pruning method, use an electric cutting machine to trim and cut the top of the tea tree cultivated in the field, so that the upper end of the tea tree branches will be cut.

2. Spray the tea tree growth internal regulation accelerator on the top of the tea tree with a sprayer. Its dosage is 50-100ml/square meter.

3. After spraying, manage according to conventional field management methods.

The action process of the present invention is such that the endophytic spirulina of the tea tree is an endophytic bacterium of the Theaceae plant, which only enters the body of the tea tree through the wound and coexists extracellularly. After the field tea tree is cut, the endophytic spirulina of the tea tree can enter the tea tree branch through the upper cut of the tea branch and colonize it when spraying. After colonization, the endophytic Helicospirilla symbioses with the tea tree and provides bacterial factors such as IAA, NH ₄ ⁺ and other active substances to promote the growth of new buds or lateral buds of the tea tree. Tea tree endophytic spirulina is a natural tea tree endophyte. It does not infect other crops and has no disease effect. It usually only colonizes older branches or leaves. There are no bacteria in new shoots and leaves, which is environmentally friendly. and food safety requirements.

The present invention has unique advantages:

1. Make full use of the biological characteristics of the endophytic Helicospira in tea trees. The endophytic spirulina of tea tree is a highly specific endophytic bacterium of Camellia genus, and it is symbiotic and pollution-free with tea tree. It can effectively produce active substances such as IAA, NH ₄ ⁺ , and siderophilic carriers that promote plant growth and development. IAA can stimulate the growth of tea tree cells, NH ₄ ⁺ can provide nitrogen source for tea trees, siderophilic carrier can absorb iron ions for tea trees, improve the disease resistance of tea trees, and cooperate with IAA and NH ₄ ⁺ to promote the growth and development of tea trees. Spraying tea tree branches with endophytic spirulina can realize high-efficiency utilization of endophytic bacterial metabolites, with low cost, convenient operation, safety and environmental protection.

2. It overcomes the shortcomings of phytohormones such as single function, short-lasting effect, and failure to effectively promote the subsequent growth and development of new shoots. The endophytic spirulina of the tea tree enters the tea tree branches and colonizes in the tea branches, and can continue to play a role without spraying wine multiple times. It not only promotes the rapid growth of new shoots, but is also expected to improve the quality of tea plants.

Description of drawings

Fig. 1 is the average length of new tea branch in the embodiment of the present invention 2-4,

Fig. 2 is the weight of 100 newborn tea branches in the embodiment of the present invention 2-4,

The control group was treated with clean water.

Detailed ways

Below in conjunction with embodiment the present invention is further described.

Example 1:

Take the endophytic Helicospirilla strains of tea trees stored at -80°C, inoculate 5 mL of nutrient broth medium (10 g tryptone, 3 g beef powder, 5 g NaCl, 10 μg/mL spectinomycin, 5 μg/mL ampicillin, add water to 1000 ml ), shake at 150 rpm, and incubate overnight at 37°C. Inject the activated bacterial solution into fresh LB liquid medium (10g tryptone, 5g yeast extract, 10g NaCl, 10μg/mL spectinomycin, 5μg/mL ampicillin at a ratio of 1:100, add water to 1000ml, pH7 .2-7.4), the culture conditions are as follows: the rotating speed of the shaker is 200 revolutions per minute, and the temperature is 28-37°C. After the bacteria were cultured for 6-8 hours and the OD ₆₀₀ value was 1.0-1.2, all the bacterial culture solution was collected, added with 1% glycerol, stored at 4°C for later use or used directly. When in use, dilute the amplified cultured tea tree endospira spirulina bacteria solution with water at a ratio of 0.5-2:1 to become a growth promoter.

Example 2: 3000mL tea tree internal regulation and growth promoting agent liquid diluted 1:2 was sprayed on 50 square meters of cut Echa No. 1 tea tree, and the new buds of the tea tree after 60 days grew well, the leaves were dark green, and the average length of new buds increased compared with the control group 3.6cm, and the weight of 100 new branches increased by 14.6 grams compared with the control group. (See Figures 1 and 2).

Example 3: 3000mL of tea tree internal regulation and growth promoting agent liquid diluted 1:1 was sprayed on 50 square meters of cut Echa No. 1 tea tree, and after 60 days, the new buds of the tea tree grew well, the leaves were dark green, and the average length of new buds increased compared to the control group 5.1cm, and the weight of 100 new branches increased by 19.7 grams compared with the control group. (See Figures 1 and 2).

Example 4: 3000mL of tea trees diluted with 2:1 were sprayed with 3000mL of tea tree growth-promoting agent liquid to spray 50 square meters of cut Echa No. 1 tea tree. After 60 days, the new buds of the tea tree grew well, the leaves were dark green, and the average length of new buds increased compared to the control group The weight of 5cm, 100 new branches increased by 19 grams compared with the control group. (See Figures 1 and 2).

Claims (1)

1. The application of a growth-promoting agent in tea tree regulation in field tea tree sprouts, characterized in that: tea tree endophytic spirulina enters the tea tree branches through the tea tree top incision to colonize, and the endophytic spirulina after colonization can pass through Internal adjustment promotes the growth of tea tree side buds, realizes weight loss in tea gardens and increases tea yield. The specific implementation steps of this application are as follows: (1) Preparation of growth-promoting agent in tea tree regulation: take the endophytic Helicospirilla strains of tea tree stored at -80°C, inoculate 5mL of nutrient broth medium, shake at 150 rpm, cultivate overnight at 37°C, and inoculate the activated Bacterial solution was inserted into fresh LB liquid medium at a ratio of 1:100 for amplified cultivation. The fermentation and cultivation conditions were as follows: shaker speed 200 rpm, temperature 28-37°C, pH 7.2-7.4, and the bacteria were fermented and cultivated. After 6-8 hours, when the OD ₆₀₀ value is 1.0, collect all the bacterial culture solution, add 1% glycerin and store it at 4°C for later use; when using, dilute the amplified culture solution with water at a ratio of 0.5-2:1, mix well and immediately Regulating growth-promoting agent in tea tree; The nutrient broth medium is 10g tryptone, 3g beef powder, 5g NaCl, add water to 1000ml, 10μg/mL spectinomycin, 5μg/mL ampicillin; The LB liquid medium is 10g tryptone, 5g yeast extract, 10g NaCl, add water to 1000ml, 10μg/mL spectinomycin, 5μg/mL ampicillin; (2) The use of the growth-promoting agent in the tea tree, according to the conventional method of pruning the tea tree, pruning and cutting the top of the tea tree cultivated in the field with an electric cutter, so that the upper ends of the branches of the tea tree are cut, and the growth-promoting agent in the tea tree is sprayed on the tea tree with a sprayer At the top, make the endophytic spirulina of the tea tree enter the tea tree branches for colonization, and the dosage is 50-100ml/square meter. After the spraying is completed, manage according to the conventional field management method.