CN105011974A - Method and device for welding biological tissue by mixed laser beam - Google Patents

Abstract

The invention proposes a method and device for welding biological tissue with mixed beam laser. Two or more lasers with different wavelengths are coupled into a welding beam and irradiated on the surface of the tissue to be welded. The lasers of different wavelengths are selectively absorbed in the thickness direction of the tissue and produce photothermal effects; use the focusing system to adjust the welding beam. The spot diameter of the welding beam on the surface of the tissue to be welded; the scanning system is used to control the moving path and speed of the welding beam on the surface of the tissue to be welded; the temperature of the welding area is collected by a photodetector, and the moving speed of the welding beam is adjusted according to the temperature. The present invention can match two or more wavelengths of laser light according to the differences in optical characteristics of different tissues of the organism or different parts of the same tissue and make them synthesized into one beam to irradiate the surface of the biological tissue, so as to realize suturing and welding of the full depth of the tissue incision. Select the mixed laser with different output modes to irradiate the incision or wound tissue, effectively reducing the area of irreversible thermal damage to the tissue.

Description

A method and device for welding biological tissue with mixed beam laser

technical field

The invention belongs to the field of laser medicine, and in particular relates to a method and a device for welding biological tissue with mixed beam laser.

Background technique

In recent decades, researchers have made remarkable achievements in the development of high-efficiency suture materials and suture instruments, and have successively developed degradable and antibacterial sutures, mechanical staplers that can simplify suture removal and shorten suturing time, tissue adhesives, etc. Mixture etc. At the same time, suturing instruments have begun to develop towards minimally invasive and automated directions, but there are certain shortcomings in such instruments, such as high price, and easy residual metal nails in the body; Degradability and other aspects still need to be further improved.

With the development of laser technology, its application in the medical field is also expanding. As a minimally invasive surgery, laser bio-tissue welding has the advantages of short operation time, fast wound healing, less inflammatory response, and less obvious scars. It is a potential tissue suture replacement technology, and some of its clinical applications have been recognized by people. . The biological tissue anastomosis welding technology using the principle of photothermal effect produced by laser acting on biological tissue has a large number of clinical needs, but the current laser biological tissue welding technology mainly focuses on the experimental research and clinical application of a single wavelength. In a single-wavelength laser bio-tissue welding device, most of the output is a fixed-size spot, which is difficult to meet the irradiation and healing requirements of incisions or wounds of different sizes. At the same time, the optical properties of biological tissues vary greatly depending on the type and location. For example, the 980nm laser is mainly absorbed by the surface layer of the skin, and a small part is absorbed by the deep tissue, while the 1064nm laser can penetrate deep into the deep tissue of the skin. The anastomosis welding of biological tissues has obvious limitations, which can easily lead to discontinuous anastomosis of the incision in the depth direction. At present, there is also a dual-beam laser bio-tissue welding technology, but the two laser beams are separated, which has the disadvantage of complex focusing, and it is difficult to coordinate the irradiation dose and movement path of the two laser beams on the incision or wound.

Contents of the invention

The object of the present invention is to provide a method and device for welding biological tissue with mixed beam laser, which can match two or more wavelengths of laser light and synthesize them into one Beam welding laser irradiates biological tissue to achieve the purpose of anastomosing the full thickness of the incision or wound of biological tissue; the present invention can also choose mixed lasers with different output modes to irradiate the incision or wound tissue, which can effectively reduce the area of irreversible thermal damage to the tissue.

In order to solve the above-mentioned technical problems, the present invention provides a method for welding biological tissue with mixed beam laser, which couples two or more laser beams with different wavelengths into one welding beam and irradiates the surface of the tissue to be welded. The laser light is selectively absorbed in the thickness direction of the tissue and produces photothermal effect.

Further, a focusing system is used to adjust the spot diameter of the welding beam irradiated on the surface of the tissue to be welded.

Further, a scanning system is used to control the moving path and speed of the welding beam on the surface of the tissue to be welded.

Further, a photoelectric detector is used to collect the temperature of the welding area, and the moving speed of the welding beam is controlled according to the temperature, so that the temperature of the welding area is always kept within a reasonable range.

Further, the indicating light is coupled with the lasers of different wavelengths to form a welding beam, and the indicating light is used to indicate the irradiation position of the welding beam.

Further, according to the physical and optical characteristics of the biological tissue to be welded, determine the wavelength and laser output mode of each laser; according to the size and depth of the incision or wound of the tissue to be welded, set the power, repetition frequency, pulse width and welding time of each laser. Beam speed etc. According to the optical characteristics of the biological tissue to be welded, the present invention matches a laser that can output a beam of a specific wavelength; according to the size and depth of the incision or wound of the biological tissue to be welded, under the known physical properties such as the specific heat capacity and density of the biological tissue, roughly estimates the biological The total heat input required by the tissue to reach 60-70°C, and then adjust the peak power, pulse width and repetition frequency of the laser and the moving speed of the welding beam to obtain the required heat input, so that the tissue to be welded will be denatured and coagulated to achieve Fit for purpose. The length of the pulse width mainly affects the thermal effect of the pulsed beam on the biological tissue. When the peak power and spot diameter are constant, the time required for different pulse widths to heat up the biological tissue to the highest temperature is different. The repetition frequency is the number of pulses of the output beam per unit time, which affects the interval time of the pulses. If the pulse interval is short, adjacent beam pulses have superimposed contributions and correlations to the thermal effect of the tissue; if the pulse interval is long enough, during the pulse interval , the temperature of the investigated point will drop to the initial temperature, then the temperature change caused by the next beam pulse is almost the same as that of the previous beam pulse. Under certain conditions of other parameters, the faster the moving speed, the less thermal energy absorbed by the biological tissue. The output mode of the laser can be divided into continuous laser and pulsed laser output. For example, choose the mixed mode of pulsed laser and continuous laser. Biological tissue enzyme activity, fixed cell tissue; the pulsed laser can further precisely control the thermal effect of the beam in the biological tissue by adjusting the pulse width and repetition frequency, so that the temperature of the biological tissue can be raised to 60-70°C, and the protein and collagen will be denatured and Coagulation, under the reasonable control of the pulse interval, the biological tissue can be cooled for a short time, instead of continuous heating to cause tissue membrane penetration or vaporization decomposition, so the mixed welding mode of pulsed laser and continuous laser has the effect of reducing the irreversible thermal damage to the biological tissue area. Effect.

Further, the moving path of the welding beam is formed according to the extension direction of the incision or the wound surface of the tissue to be welded, and the scanning system applies the welding beam to the part of the biological tissue to be welded according to the moving path.

The present invention also provides a device for laser welding biological tissue with a mixed beam, including a laser generator system, a laser beam combiner, a laser collimator, a focusing system and a scanning system; the laser generator system includes two or two More than two lasers are used to emit lasers of different wavelengths; the laser beam combiner is used to couple the lasers of different wavelengths into a welding beam; the focusing system is used to adjust the welding beam to be irradiated on Spot diameter on the tissue surface; the scanning system is used to control the moving path and speed of the welding beam on the tissue surface to be welded.

Further, in the laser generator system, one of the lasers is a micro laser, which is used to output indicating light to indicate the irradiation position of the welding beam.

Further, the device further includes a photodetector and a control system, the photodetector is used to collect temperature signals of the welding area, and the control system controls the moving speed of the welding beam according to the temperature signals.

The laser generator system outputs a number of laser beams with specific wavelengths. The laser beam combiner can couple multiple laser beams into one beam, and then irradiate the spot of a certain size to the incision or wound of biological tissue through the focusing system and scanning system. The photodetector collects The temperature signal of the incision or wound tissue welding area is fed back to the control system, and the moving speed is adjusted online, so as to finally achieve a high-quality welding effect with less thermal damage, high anastomosis continuity and fast recovery. In addition, after multiple laser beams are coupled into one beam, it is easier to adjust the focus and manipulate the moving path and speed of the beam on the incision or wound tissue than to control the two laser beams separately in space, which shortens the welding time.

Compared with the prior art, the present invention has significant advantages in that:

(1) The present invention couples light beams of different wavelengths into a laser beam, and when irradiating incision or wound tissue, it can meet the different requirements of different optical characteristics of biological tissues, and can make lasers of different wavelengths interact with different depths of tissues to produce photothermal effects, To achieve continuous matching in the depth direction;

(2) The present invention can choose a mixed mode of different laser output modes, and pulsed laser has the therapeutic effect of reducing the area of irreversible thermal damage to biological tissue compared with continuous laser;

(3) In the present invention, after mixing multiple beams of laser light into one beam, compared with irradiating biological tissue with multiple separate laser beams, it is easy to keep the light beams of different wavelengths concentrated on the same position on the incision or wound tissue surface at the same time, so that The control of the moving path of the mixed beam is simple and easy;

(4) The present invention introduces a micro-laser generator to emit indicating light, which is synthesized with the main laser beam, which is convenient for finding the starting position of the light spot irradiated on the incision or wound of the biological tissue, shortens the welding time, and indicates that the wavelength λ of the red light is 650nm. The output power is less than 6mW, the impact on the test or postoperative results is negligible, and the laser power can be turned off during the welding process;

(5) The focus system can adjust the spot diameter of the mixed beam, which can be continuously adjusted within 0.5-5mm, and can meet the welding purpose of different sizes of incisions. Compared with two separate laser beams irradiating tissues, one focus system is reduced and the adjustment is convenient;

(6) Under the control of the control system, the scanning system can realize basic graphics such as straight lines, circles, and polygons, which basically meet the welding purposes of incisions or wound tissues of different shapes and spaces, and can be integrated into the control system to quickly adjust the beam moving speed online ;

(7) When the biological tissue reaches a certain temperature, the structures such as collagen or protein begin to denature and solidify, so that the two ends of the tissue are bonded and connected. The present invention uses a photodetector to collect the optical signal of the welding area, converts it into a temperature signal, and feeds it back to the control system , adjust the driving circuit to change the moving speed of the beam, so that the temperature of biological tissue welding is in the optimal range, while ensuring the basic welding temperature and reducing the high temperature residence time in the welding area, and finally achieve the welding effect of high anastomosis continuity and small irreversible thermal damage area.

Description of drawings

Fig. 1 is a schematic structural diagram of a device for welding biological tissue with a mixed beam laser according to the present invention.

Fig. 2 is a schematic structural diagram of the scanning system in the device of the present invention.

Fig. 3 is a flow chart of the method for welding biological tissue with mixed beam laser according to the present invention.

Fig. 4 is a schematic diagram of the moving path of the light beam in the embodiment of the present invention.

Detailed ways

It is easy to understand that, according to the technical solution of the present invention, without changing the essence of the present invention, those skilled in the art can imagine various implementations of the method and device for laser welding biological tissue with mixed beams of the present invention. Therefore, the following specific embodiments and drawings are only exemplary descriptions of the technical solution of the present invention, and should not be regarded as the entirety of the present invention or as a limitation or limitation on the technical solution of the present invention.

Referring to Fig. 1, the mixed-beam laser welding biological tissue device of this embodiment mainly includes a laser generator system 1, an output optical fiber 2, a 3×1 laser beam combiner 3, a laser collimator 4, a focusing system 5, and a scanning system 6 , photodetector 10, control system 11.

The laser generator system 1 is equipped with three lasers, wherein the first laser 1-1 and the second laser 1-2 output lasers of different wavelengths, which can be realized by using pulsed lasers or continuous lasers of different wavelengths; the third laser 1-3 is a miniature The laser is used to output red light to indicate where the welding beam should hit the targeted incision or wound. The laser light emitted by the laser generator system 1 is sent to the laser beam combiner 3 through the output optical fiber 2 .

The laser beam combiner 3 is used to couple the lasers emitted by the above three lasers into a laser beam, and under the joint action of the collimator 4 and the focusing system 5, it outputs a beam with a certain diameter and a small divergence angle, and then the beam enters the scanning System 6.

The structure of scanning system 6 is shown in Figure 2, mainly by two optical scanning mirrors, X-scanning motor, Y-scanning motor, X-driver circuit and Y-driver circuit, control system 11 is through X-driver circuit and Y - The driving circuit respectively drives the X-scanning motor and the Y-scanning motor, thereby controlling the deflection of the laser beam in the X-Y plane, and completing the moving path of the beam on the two-dimensional plane. The scanning system can realize straight line, Basic graphics such as circles and polygons can meet the purpose of anastomotic welding of incisions or wound tissues of different shapes and spaces.

The control system 11 is used to preset the moving path and speed of the beam, and control the scanning system 6 to apply the welding beam 7 to the target incision or wound surface of the biological tissue 8 according to the moving path and speed.

The photodetector 10 is used to collect the optical signal of the biological tissue welding area during the welding process and convert it into a temperature signal. The control system 11 analyzes the temperature signal. If the temperature of the welding area does not reach the preset value, the welding beam is slowed down through the driving circuit. If the moving speed of 7 exceeds the preset value, the moving speed of welding beam 7 will be accelerated through the driving circuit.

In conjunction with Fig. 3, in conjunction with above-mentioned device, the present invention uses the method for laser welding biological tissue of mixed beam to be:

Step 1. According to the physical and optical characteristics of different biological tissues, match the laser generator with specific wavelength to emit the welding beam, and select the output mode of the laser. The mixed laser output mode includes continuous and pulse mixed output mode, continuous and continuous mixed output mode , pulse and pulse mixed output modes, etc. The optical properties of different parts of biological tissue are quite different. For example, for skin tissue, 980nm and 1064nm lasers are easy to be absorbed by the epidermis and dermis (or deep layer) of the skin respectively, so a 980nm continuous laser and a 1064nm pulsed laser can be selected. Lasers of different wavelengths synthesize a beam of irradiated tissue, which mainly acts on the epidermis and dermis (or deep layer) of the skin respectively, and produces photothermal effect to denature and coagulate the tissue, which can achieve the purpose of anastomotic welding of the full thickness of the skin incision. The parameters of 980nm continuous laser are mainly power, and the parameters of 1064nm pulsed laser are mainly peak power, pulse width and repetition frequency. The continuous laser ensures that the beam provides continuous heat input in the tissue, which increases the temperature of the tissue by 10-20°C, weakens the activity of tissue enzymes, and fixes the tissue; the pulsed laser can further precisely control the heat of the beam in the tissue by adjusting the pulse width and repetition frequency. The effect is to raise the temperature of the tissue to 60-70°C, denaturation and coagulation of protein and collagen, and under the reasonable control of the pulse interval, the tissue can be cooled for a short time, instead of continuous heating to cause tissue membrane penetration or vaporization decomposition, so The hybrid welding mode of pulsed laser and continuous laser has the therapeutic effect of reducing the area of irreversible thermal damage to biological tissue.

Step 2. According to the size, depth and area of the incision or wound tissue, and with known physical properties such as specific heat capacity and density of the tissue, roughly estimate the total heat input required for the tissue to reach 60-70°C. The parameters of the first laser 1-1 and the second laser 1-2 are preset to output a welding beam that denatures and coagulates the tissue, and estimates the moving speed of the welding beam. CW laser parameters mainly include power, while pulsed laser parameters mainly include peak power, pulse width and repetition frequency, etc. Power plays the most important role in heat input, high power leads to faster temperature rise of tissue; low power, vice versa. The length of the pulse width mainly affects the thermal effect of the pulsed beam on the tissue. When the peak power and spot diameter are constant, the time required for different pulse widths to heat up the tissue to the highest temperature is different. The repetition frequency is the number of pulses of the output beam per unit time, which affects the interval time of the pulses. If the pulse interval is short, adjacent beam pulses have superimposed contributions and correlations to the thermal effect of the tissue; if the pulse interval is long enough, during the pulse interval , the temperature of the investigated point will drop to the initial temperature, then the temperature change caused by the next beam pulse is almost the same as that of the previous beam pulse. Under certain other parameters, the faster the moving speed, the less heat energy absorbed by the biological tissue; the slower the moving speed, the longer the tissue stays at high temperature, and the larger the area of irreversible thermal damage may be.

Step 3, according to the incision or wound size of the tissue, modulate the focusing system 5, and set the spot diameter of the welding beam 7;

Step 4, place the biological tissue sample on the working platform 9, use the micro laser generator 1-3 to output the indicating red light, and use the control system 11 to find the starting position of the light spot irradiating the target tissue;

Step 5. According to the extension direction of the tissue incision or wound, under the guidance of red light, the control system 11 uses programming software to preset the moving path of the welding beam;

Step 6. After all the above-mentioned parameters are set, turn off the micro laser generator 1-3, turn on the power supply of the first laser 1-1 and the second laser 1-2, run the control system 11, and the welding beam 7 starts to cut or cut the target tissue. Wound surface 8 is laser welded;

Step 7. During the laser welding process, the photodetector 10 collects the optical signal of the welding area, converts it into a temperature signal under the control system 11, and judges whether the preset value is reached. If not, control the driving circuit to slow down the moving speed of the welding beam ; If it exceeds a certain range of the preset value, the moving speed will be accelerated;

Step 8. After the welding process is completed, turn off the power of the system and remove the target tissue.

The laser collimator 4 and the focusing system 5 in the above step 3 can shape the beam coupled by the beam combiner 3, output a beam with a diameter continuously adjustable in the range of 0.5-5mm, and the divergence angle of the beam is as small as possible . The output red light wavelength λ of the miniature laser generator 1-3 in the above step 4 is 650nm, the output power is less than 6mW, and the influence of the welding process on the result is negligible. In the above step 5, the scanning system 6 can realize two modes of "point scanning" and "line scanning" of the light beam. For wound tissue with a small area, the "point scanning" mode can be selected; for longer tissue incisions, the "point scanning" mode can be selected; line scan mode.

The present invention will be further described below in conjunction with experiments.

Experiment 1

Taking fresh isolated pork with full-thickness skin as an example, the sample size is 100×100×10mm, and three 50mm long incisions with intervals of 20mm are made with a scalpel, and the depth goes through the whole skin layer (including epidermis and dermis, about 1.5mm thick). On the welding surface of each incision, 3 stitches of equidistant sutures are used to draw the incision to avoid excessive opening and affect laser welding.

Design the basic parameters of the welding beam. In the laser generator system, the peak power of 1064nm pulse laser 1-1 is set to 5W, the pulse width is 10ms, the repetition frequency is 20HZ, and the output power is 1W, and the output power of 980nm continuous laser 1-2 is set to 1W. Adjust the focus system 5 to make the output spot diameter reach 2mm.

Design the moving path and speed of the beam, place the sample tissue on the workbench 9 and fix it, turn on the power of the micro laser generator 1-3, output red light, and find the starting position of the beam at the incision. The computer control software of the operating control system 11 edits the moving path and speed of the welding beam according to the extension direction of the kerf, as shown in Figure 4, the solid line part is the moving path of the beam on the kerf surface, and the speed is 2mm/s; the dotted line part is the beam moving on the tissue The speed of the moving path on the surface of the normal part is 30mm/s. In view of the fast moving speed of the beam in this area, the influence on the actual result can be ignored. After the moving path and speed are set, turn off the power supply of the micro laser generator 1-3, start the first laser 1-1 and the second laser 1-2, run the control system 11, and the welding beam 7 starts to laser the target tissue incision 8 welding.

During the welding process, the photodetector 10 collects the light signal of the welding area, converts it into a temperature signal under the control system, and judges whether it reaches 60-70°C, if not, performs compensation calculation, and controls the driving circuit to slow down the moving speed of the beam; If it exceeds 60-70°C, speed up the moving speed of the beam, and keep the temperature of the target area within the range of 60-70°C during the whole process.

After welding, turn off all the power of the system and remove the sample tissue.

Experiment 2

Take the sciatic nerve of a living rat as an example.

Design the basic parameters of the welding beam. In the laser generator system, match the argon ion laser 1-1 with a wavelength of 514.5nm and the He-Ne laser 1-2 with a wavelength of 633nm. Both lasers output continuous laser light. The power of Argon ion laser 1-1 is set to 500mW, and the power of He-Ne laser 1-2 is set to 200mW. Adjust the focusing system 5 to make the output spot diameter reach 1mm.

After the live rats were anesthetized, depilated, routinely disinfected, incised the skin and muscle layers, freed the sciatic nerve, cut off the nerve trunk neatly in the middle, and then performed the end-to-end fusion of the nerve segments under the operating microscope, and used a small amount of blood obtained from the tail of the rat Drop on the anastomotic end. The white mice were fixed in a special cage and placed on the workbench 9. The micro laser generators 1-3 were powered on to output red light, and the position of the light spot was adjusted to accurately irradiate the target point on the nerve trunk. Run the computer control software of the control system 11, and edit the speed of the welding beam to be 0 (ie point scanning). Turn off the power supply of the micro laser generator 1-3, start the first laser 1-1 and the second laser 1-2, run the control system 11, and the welding beam 7 starts to perform laser welding on the tissue incision 8.

During the welding process, the photoelectric detector 10 collects the light signal of the welding area, converts it into a temperature signal under the control system, and displays it on the display screen of the control system 11, and manually observes the display screen, if it reaches 60-70 ° C, then modulate the cage or The position of the sciatic nerve trunk is such that the welding beam 7 is irradiated at the next point of the incision of the nerve trunk, and finally irradiates 4 points, each of which is 90° apart.

After the welding is completed, turn off all power supplies of the system and remove the experimental samples.

Experiment 3

Take the liver of an average domestic dog as an example.

Laparotomy is performed on an ordinary live domestic dog after anesthesia to expose the liver, and a 30mm long and 5mm deep incision is cut along a straight line on the surface with a scalpel. On the welding surface of the incision, a stitch is sutured in the middle to prevent the incision from opening too much And affect laser welding.

Design the basic parameters of the welding beam. In the laser generator system, match the Nd:YAG pulsed laser 1-1 with a wavelength of 1064nm and the continuous laser 1-2 with a wavelength of 1350nm. The peak power of laser 1-1 is set to 10W, the pulse width is 20ms, the repetition frequency is 20HZ, the output power is 4W, and the output power of laser 1-2 is set to 2W. Adjust the focus system 5 to make the output spot diameter reach 3mm.

Design the moving path and speed of the light beam, place the domestic dog together with the liver tissue on the workbench 9 and fix it, turn on the power of the micro laser generator 1-3, output red light, and find the starting position of the light beam at the incision. The computer control software of the operating control system 11 edits the moving path and speed of the welding beam according to the extension direction of the incision. The welding beam performs "point scanning" on the incision, with an overlap of 1mm between two points, and the irradiation time of each point is 10s. The speed from one point to the next is 10mm/s. After the moving path and speed are set, turn off the power supply of the micro laser generator 1-3, start the first laser 1-1 and the second laser 1-2, run the control system 11, and the welding beam 7 starts to laser the target tissue incision 8 welding.

During the welding process, the photodetector 10 collects the light signal of the welding area, converts it into a temperature signal under the control system, and judges whether it reaches 60-70°C, if not, performs compensation calculation, and controls the driving circuit to slow down the moving speed of the beam; If it exceeds 60-70°C, speed up the moving speed of the beam, and keep the temperature of the target area within the range of 60-70°C during the whole process.

After the welding is completed, turn off all power supplies of the system and remove the experimental samples.

Claims (10)

1. the method with mixed light beam laser weld biological tissue, it is characterized in that, the laser coupled two or more with different wave length is expose to tissue surface to be welded after a branch of welding light beam, and the laser of different wave length is produced photo-thermal effect by selective absorbing on tissue thickness direction.

2. use the method for mixed light beam laser weld biological tissue as claimed in claim 1, it is characterized in that, use focusing system to adjust the spot diameter of described welding light beam irradiation at tissue surface to be welded.

3. use the method for mixed light beam laser weld biological tissue as claimed in claim 1, it is characterized in that, use scanning system to control described welding light beam in the mobile route of tissue surface to be welded and speed.

4. use the method for mixed light beam laser weld biological tissue as claimed in claim 1, it is characterized in that, use photodetector to gather the temperature of welding region, according to the translational speed of described temperature adjustment welding light beam, welding region temperature is remained in reasonable interval.

5. as described in claim 1,2,3 or 4, use the method for mixed light beam laser weld biological tissue, it is characterized in that, be a branch ofly weld light beam by pilot light and the laser coupled of described different wave length, described pilot light is used to indicate the irradiation position of described welding light beam.

6. use the method for mixed light beam laser weld biological tissue as claimed in claim 5, it is characterized in that, according to physical characteristic and the optical characteristics of biological tissue to be welded, determine wavelength and the Laser output pattern of each laser; According to size and the degree of depth of tissue cut to be welded or wound surface, set the translational speed etc. of the power of each laser, repetition rate, pulsewidth and welding light beam.

7. use the method for mixed light beam laser weld biological tissue as claimed in claim 5, it is characterized in that, form the mobile route of described welding light beam according to the otch of tissue to be welded or wound surface extension direction, according to mobile route, welding light beam is acted on biological tissue position to be welded by scanning system.

8. with a device for mixed light beam laser weld biological tissue, it is characterized in that, comprise laser generator system, laser bundling device, laser aligner, focusing system and scanning system;

Described laser generator system comprises two or more laser instrument, for launching the laser of different wave length;

It is a branch of welding light beam that described laser bundling device is used for the laser coupled of described different wave length;

Described focusing system is for adjusting the spot diameter of described welding light beam irradiation at tissue surface to be welded;

Described scanning system welds light beam in the mobile route of tissue surface to be welded and speed for controlling.

9. device as claimed in claim 1, it is characterized in that, in described laser generator system, one of them laser instrument is microlaser, for exporting pilot light, the irradiation position of instruction welding light beam.

10. device as claimed in claim 1, is characterized in that, also comprise photodetector and control system, and described photodetector is for gathering the temperature signal of welding region, and described control system controls the translational speed of welding light beam according to described temperature signal.