CN104974198A - Process of extracting tannin from pine bark - Google Patents

Abstract

The invention discloses a process for extracting tannin from pine bark, the method comprising the following steps: a process for extracting tannin from pine bark, the process comprising the following steps: (1) pretreatment: air-drying the pine bark naturally After crushing through a 100-mesh sieve, the pine bark powder is obtained, which is for subsequent use; (2) microwave extraction: take the standby pine bark powder, place it in a round-bottomed flask with a reflux condenser, then add the extract, and place the round-bottomed flask Put it into a microwave reactor, and carry out microwave extraction for 1 min at a microwave power of 80-85W and a temperature of 45°C to obtain a mixed extract; (3) Purification: After cooling the obtained mixed extract to room temperature, perform suction filtration, and After the filtrate is vacuum-concentrated at 40°C, it is vacuum-dried at 60°C until the quality is constant to obtain the birch tannin extract. The method of the invention has the advantages of simple and convenient operation, high extraction efficiency, etc., provides a new approach for the utilization of pine bark, and increases the added value of pine bark.

Description

Process of extracting tannin from pine bark

technical field

The invention relates to the technical field of plant extraction, in particular to a process for extracting tannin from pine bark.

Background technique

Pine bark contains a variety of substances, and it is not uncommon to extract substances from pine bark. At present, the research on pine bark extract in China focuses on the anti-oxidant substances in pine bark extract, anthocyanins (proanthocyanidin oligomers) (OPCs) , the antioxidant capacity of this substance is 20 times that of vitamin C and 50 times that of vitamin E. As well-known antioxidants, OPCs can effectively suppress the action of free radicals, which play an important role in degenerative diseases, cardiovascular diseases, blurred vision, sunburn and premature aging.

However, pine bark also contains a variety of substances that can be extracted. Pine bark contains more tannins. Tannins are also known as tannic acid, tannins, and tannins. It widely exists in the leaves, fruits, roots and bark of plants, and is also the main component of galls produced by trees attacked by insects; it is mainly used in medicine, food, tanning, daily chemicals, water treatment and other fields. Tannin is a highly polymerized compound among polyphenols. It is a yellow or brownish yellow amorphous loose powder. The color gradually becomes darker in the air and has strong hygroscopicity. It is insoluble in ether, benzene, and chloroform, and easily soluble in water and ethanol. , Acetone, the aqueous solution has astringent taste. The chemical composition of tannin is relatively complicated, and it can be roughly divided into two types: one is hydrolyzed tannin, which is a compound formed by polyphenolic acid and sugar mainly through ester bonds, such as gallic acid tannin and ellagic acid tannin; It is a condensed tannin, and its important components are flavanols and their derivatives, such as quebracho tannin, wattle bark tannin and larch tannin. Tannins with a specific molecular structure have physiological activity and can interact with proteins, polysaccharides, nucleic acids, etc. in organisms. The effect of tannin on the human body is dual. Excessive tannin in food will produce anti-nutritional effects, such as reducing the nutritional value of protein and affecting the effective utilization of VA and Vb12. In traditional medicine, tannin can be used internally to treat gastrointestinal bleeding and dysentery, and externally used for local hemostasis and wound protection to prevent infection and inflammation; recent studies have found that tannin also has antibacterial, anti-viral, anti-allergic, anti-oxidative, anti-aging, and preventive effects. Cardiovascular disease, anti-tumor and immune-stimulating effects. Tannin can be used as a functional ingredient in the food industry to make health care products, food additives, flavors, etc.; in cosmetics, it mainly plays the role of astringent, sunscreen, whitening, anti-wrinkle, moisturizing, and antiseptic; in the leather industry, Tannin is the main component of extract. Therefore, using pine bark to extract tannins can increase the added value of pine bark, which has important economic and social benefits.

Contents of the invention

In order to realize the purpose of the present invention, the present invention provides a kind of technique that extracts tannin from pine bark, and the method of the present invention has advantages such as easy and simple operation, high extraction efficiency, provides a kind of new approach for the utilization of pine bark, improves the tannin of pine bark additional value.

In order to achieve the above object, the technical scheme adopted in the present invention is as follows:

A process for extracting tannin from pine bark, the process comprises the following steps:

(1) pretreatment: the pine bark is naturally air-dried and then pulverized through a 100-mesh sieve to obtain pine bark powder for subsequent use;

(2) Microwave extraction: take the standby pine bark powder, place it in a round-bottomed flask with a reflux condenser, then add the extract, then put the round-bottomed flask into a microwave reactor, and the microwave power is 80-85W , and the temperature was 45°C for microwave extraction for 1 min to obtain a mixed extract;

(3) Purification: the obtained mixed extract was cooled to normal temperature, then suction filtered, the filtrate was concentrated in vacuum at 40°C, and then vacuum-dried at 60°C until the quality was constant to obtain the birch tannin extract.

In the present invention, as a further illustration, the mass ratio of the extraction liquid and the pine tree powder in the step (2) is 1:20-30.

In the present invention, as a further illustration, the extract is a mixed solution with a mass ratio of ethanol: acetone: water of 10-15:8-10:0.3-0.5.

In the present invention, as a further illustration, the specification of the round bottom flask is 250mL.

The beneficial effects of the present invention are:

1. The present invention carries out microwave extraction of tannin substance in pine bark with ethanol, acetone, water mixed liquid level extraction solvent, shortens extraction time by allocating optimal material-liquid ratio, extraction temperature, microwave power, extraction time and other factors , increase the extraction rate of active ingredients, and compared with traditional thermal extraction, microwave-assisted extraction has a shorter time and higher extraction efficiency.

2. The present invention has the advantages of simple process, easy operation and high extraction rate.

3. Tannin Tannin has effects such as hemostasis, inhibition of microorganisms, anti-allergic, anti-mutation, anti-cancer, anti-tumor, anti-aging, and the method provided by the present invention extracts tannin and can fully exert the potential potential of excavating pine bark harvesting and processing residues. Economic value provides a new way for farmers to increase their income.

Detailed ways

The present invention will be further described below through specific examples, so that the advantages and features of the present invention can be more easily understood. It should be understood that the examples of the present invention are only used for the present invention, rather than limiting the present invention.

Example 1

A process for extracting tannin from pine bark, the process comprises the following steps:

(1) pretreatment: the pine bark is naturally air-dried and then pulverized through a 100-mesh sieve to obtain pine bark powder for subsequent use;

(2) Microwave extraction: take the standby pine bark powder, place it in a 250mL round-bottomed flask with a reflux condenser, then add the mixed solution according to ethanol: acetone: water mass ratio of 10:8:0.3 as the extract, Among them, the mass ratio of the extract to the pine tree powder is 1:20, and then the round bottom flask is put into a microwave reactor, and the microwave power is 80W, and the temperature is 45°C, and the microwave extraction is carried out for 1min to obtain the mixed extract;

(3) Purification: the obtained mixed extract was cooled to normal temperature, then suction filtered, the filtrate was concentrated in vacuum at 40°C, and then vacuum-dried at 60°C until the quality was constant to obtain the birch tannin extract.

After calculation, the final total amount of tannins obtained in this embodiment is 110.05mg/100g.

Example 2

A process for extracting tannin from pine bark, the process comprises the following steps:

(1) pretreatment: the pine bark is naturally air-dried and then pulverized through a 100-mesh sieve to obtain pine bark powder for subsequent use;

(2) Microwave extraction: take by weighing the spare pine bark powder, place in the 250mL round-bottomed flask with reflux condenser, then add according to ethanol: acetone: the mixed solution that water mass ratio is 3:2:0.1 is extraction liquid, Among them, the mass ratio of the extract to the pine tree powder is 1:30, and then the round bottom flask is put into a microwave reactor, and the microwave power is 85W, and the temperature is 45°C, and the microwave extraction is carried out for 1min to obtain the mixed extract;

(3) Purification: the obtained mixed extract was cooled to normal temperature, then suction filtered, the filtrate was concentrated in vacuum at 40°C, and then vacuum-dried at 60°C until the quality was constant to obtain the birch tannin extract.

After calculation, the total amount of tannins obtained in this embodiment is 112.35mg/100g.

Example 3

A process for extracting tannin from pine bark, the process comprises the following steps:

(1) pretreatment: the pine bark is naturally air-dried and then pulverized through a 100-mesh sieve to obtain pine bark powder for subsequent use;

(2) Microwave extraction: take by weighing the spare pine bark powder, place in the 250mL round-bottomed flask with reflux condenser, then add according to ethanol: acetone: the mixed solution that water mass ratio is 13:9:0.4 is extracting liquid, Among them, the mass ratio of the extract to the pine tree powder is 1:25, and then the round bottom flask is put into a microwave reactor, and the microwave power is 81W, and the temperature is 45°C, and the microwave extraction is carried out for 1min to obtain the mixed extract;

(3) Purification: the obtained mixed extract was cooled to normal temperature, then suction filtered, the filtrate was concentrated in vacuum at 40°C, and then vacuum-dried at 60°C until the quality was constant to obtain the birch tannin extract.

After calculation, the final total amount of tannins obtained in this embodiment is 119.52mg/100g.

Example 4

A process for extracting tannin from pine bark, the process comprises the following steps:

(1) pretreatment: the pine bark is naturally air-dried and then pulverized through a 100-mesh sieve to obtain pine bark powder for subsequent use;

(2) Microwave extraction: take the standby pine bark powder, place it in a 250mL round-bottomed flask with a reflux condenser, then add the mixed solution according to ethanol: acetone: water mass ratio of 14:9:0.3 as the extract, Among them, the mass ratio of the extract to the pine tree powder is 1:29, and then the round bottom flask is put into a microwave reactor, and the microwave power is 84W, and the temperature is 45°C, and the microwave extraction is carried out for 1min to obtain the mixed extract;

(3) Purification: the obtained mixed extract was cooled to normal temperature, then suction filtered, the filtrate was concentrated in vacuum at 40°C, and then vacuum-dried at 60°C until the quality was constant to obtain the birch tannin extract.

After calculation, the total amount of tannins obtained in this embodiment is 120.11mg/100g.

The pine bark extraction tannin provided by the present invention has simple extraction process, low cost, and high extraction rate. Analysis by high-performance liquid chromatography shows that the finished product has high tannin content, high purity, and high biological activity. application value.

Claims (4)

1. extract a technique for tannin from Cortex Pini, it is characterized in that, this technique comprises the following steps:

(1) pre-treatment: pulverized 100 mesh sieves after air-dry for Cortex Pini natural drying, obtain Cortex Pini powder, for subsequent use;

(2) microwave extraction: take Cortex Pini powder for subsequent use, be placed in the round-bottomed flask of band reflux condensing tube, then extracting solution is added, again round bottom flask is entered in microwave reactor, be 80-85W at microwave power, temperature is carry out microwave extraction 1min under 45 DEG C of conditions, obtains mixed extract;

(3) purifying: will obtain carrying out suction filtration after mixed extract is cooled to normal temperature, after vacuum concentration at filtrate 40 DEG C, vacuum-drying is to constant mass at 60 DEG C, white birch tanning extract.

2. a kind of technique extracting tannin from Cortex Pini according to claim 1, is characterized in that: extracting solution described in described step (2) and pine tree powder are 1:20-30 according to mass ratio.

3. a kind of technique extracting tannin from Cortex Pini according to claim 1, is characterized in that: described extracting solution is according to ethanol: acetone: quality is than the mixed solution for 10-15:8-10:0.3-0.5.

4. a kind of technique extracting tannin from Cortex Pini according to claim 3, is characterized in that: the specification of described round-bottomed flask is 250mL.