CN104961821A - Method for extracting gonadotrophic hormone from human urine - Google Patents
Method for extracting gonadotrophic hormone from human urine Download PDFInfo
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- CN104961821A CN104961821A CN201410362136.7A CN201410362136A CN104961821A CN 104961821 A CN104961821 A CN 104961821A CN 201410362136 A CN201410362136 A CN 201410362136A CN 104961821 A CN104961821 A CN 104961821A
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- 229940088597 hormone Drugs 0.000 title claims abstract description 32
- 239000005556 hormone Substances 0.000 title claims abstract description 32
- 210000002700 urine Anatomy 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 22
- 230000001456 gonadotroph Effects 0.000 title abstract 4
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 15
- 239000012535 impurity Substances 0.000 claims abstract description 9
- 238000005342 ion exchange Methods 0.000 claims abstract description 5
- 238000001556 precipitation Methods 0.000 claims abstract description 4
- 238000001914 filtration Methods 0.000 claims abstract description 3
- 239000007788 liquid Substances 0.000 claims description 21
- 239000003960 organic solvent Substances 0.000 claims description 17
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical group CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 8
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 7
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 7
- 108010057021 Menotropins Proteins 0.000 claims description 7
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 239000002244 precipitate Substances 0.000 claims description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 4
- 229920002684 Sepharose Polymers 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000013019 agitation Methods 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 230000035935 pregnancy Effects 0.000 claims description 4
- 239000011780 sodium chloride Substances 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 239000005995 Aluminium silicate Substances 0.000 claims description 3
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims description 3
- 239000005695 Ammonium acetate Substances 0.000 claims description 3
- 235000012211 aluminium silicate Nutrition 0.000 claims description 3
- 229940043376 ammonium acetate Drugs 0.000 claims description 3
- 235000019257 ammonium acetate Nutrition 0.000 claims description 3
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 239000012043 crude product Substances 0.000 claims description 2
- 239000000706 filtrate Substances 0.000 claims description 2
- 238000004255 ion exchange chromatography Methods 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000004440 column chromatography Methods 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- ZDRRIRUAESZNIH-BZGUUIOASA-N (2s)-1-[(4r,7s,10s,13s,16s,19r)-19-amino-7-(2-amino-2-oxoethyl)-13-[(2s)-butan-2-yl]-10-[(1r)-1-hydroxyethyl]-16-[(4-hydroxyphenyl)methyl]-6,9,12,15,18-pentaoxo-1,2-dithia-5,8,11,14,17-pentazacycloicosane-4-carbonyl]-n-[(2s)-1-[(2-amino-2-oxoethyl)amino]- Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@H](C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)[C@@H](C)O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 ZDRRIRUAESZNIH-BZGUUIOASA-N 0.000 description 8
- 108010047196 Urofollitropin Proteins 0.000 description 8
- 239000000047 product Substances 0.000 description 8
- 229960004371 urofollitropin Drugs 0.000 description 8
- 108010073521 Luteinizing Hormone Proteins 0.000 description 6
- 102000009151 Luteinizing Hormone Human genes 0.000 description 6
- 230000028327 secretion Effects 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 229960004407 chorionic gonadotrophin Drugs 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 102000003886 Glycoproteins Human genes 0.000 description 3
- 108090000288 Glycoproteins Proteins 0.000 description 3
- 210000004246 corpus luteum Anatomy 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 2
- 239000003098 androgen Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000003416 augmentation Effects 0.000 description 2
- 238000004166 bioassay Methods 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 230000003325 follicular Effects 0.000 description 2
- 208000000509 infertility Diseases 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 231100000535 infertility Toxicity 0.000 description 2
- 230000002611 ovarian Effects 0.000 description 2
- 210000002394 ovarian follicle Anatomy 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 206010000242 Abortion threatened Diseases 0.000 description 1
- 206010002659 Anovulatory cycle Diseases 0.000 description 1
- 108010001857 Cell Surface Receptors Proteins 0.000 description 1
- 206010011498 Cryptorchism Diseases 0.000 description 1
- 206010013908 Dysfunctional uterine bleeding Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108010079345 Follicle Stimulating Hormone Proteins 0.000 description 1
- 102000012673 Follicle Stimulating Hormone Human genes 0.000 description 1
- 102000006771 Gonadotropins Human genes 0.000 description 1
- 108010086677 Gonadotropins Proteins 0.000 description 1
- 208000008899 Habitual abortion Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 208000005985 Threatened Abortion Diseases 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 201000000160 cryptorchidism Diseases 0.000 description 1
- 230000001076 estrogenic effect Effects 0.000 description 1
- 201000003585 eunuchism Diseases 0.000 description 1
- 229940028334 follicle stimulating hormone Drugs 0.000 description 1
- 210000001733 follicular fluid Anatomy 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 239000002622 gonadotropin Substances 0.000 description 1
- 229940094892 gonadotropins Drugs 0.000 description 1
- 210000002503 granulosa cell Anatomy 0.000 description 1
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 208000021267 infertility disease Diseases 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 230000000938 luteal effect Effects 0.000 description 1
- 208000037106 male hypogonadism Diseases 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 102000006240 membrane receptors Human genes 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000001817 pituitary effect Effects 0.000 description 1
- 210000002826 placenta Anatomy 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 239000000583 progesterone congener Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 210000003684 theca cell Anatomy 0.000 description 1
- 210000002993 trophoblast Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Endocrinology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Reproductive Health (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a method for extracting gonadotrophic hormone from human urine. The method is characterized by comprising the following steps: step 1, adjusting a pH value so as to allow impurities to be precipitated; step 2, carrying out filtering to remove the impurities and then carrying out ultrafiltration and concentration; step 3, carrying out ion exchange column chromatography; step 4, carrying out precipitation and impurity removal; and step 5, carrying out precipitation and drying so as to obtain a crude gonadotrophic hormone product. The method for extracting gonadotrophic hormone from human urine has the advantages of simple process, high product yield, low production cost, good product purity, high titer, etc. and enables a plurality of steps to be omitted in subsequent extraction of a high purity product.
Description
Technical field
The present invention relates to a kind of chemicals extraction process, especially a kind of method extracting gonad-stimulating hormone from people's urine.
Background technology
Gonad-stimulating hormone (gonadotropins) regulates vertebrates gonad development, promotes the glycoprotein hormones that sexual hormoue generates and secretes.As the Menotropins (HMG) of prepituitary gland secretion, the chorionic-gonadotropin hormone (HCG of Human plactnta secretion, be called for short chorionic gonadotrophin), short corpus luteum growth element (LH) etc., all be widely used in infertile treatment, they have similar structure, be made up of α, β two peptide chains respectively, wherein the sequence of α chain is on all four.Difference between different albumen, in the sequence of β chain, is also reflected in different space structures and the combination to different cell surface receptor.
Menotropins (HMG) is by extracting the class reproductive hormone obtained in menopausal women urine, mainly containing Urofollitropin (FSH, Follicle-Stimulating Hormone) and interstitialcellstimulating hormone (ICSH) (LH, Luterinizing Hormone) composition.Urofollitropin is the class glycoprotein being synthesized by anterior pituitary basophil cell, secrete, and be made up of ɑ, β two subunits, ɑ has 85 amino acid, and β has 115 amino acid, and specificity is made up of β subunit, molecular weight about 33000.The main Physiological Function of Urofollitropin is the various Growth of Cells, the growth that promote ovarian follicle, in order to the maturation of ovocyte.The effect of Urofollitropin comprises: around promoting ovarian follicle, mesenchymal cell is divided into inside and outside two-layer thecacells and synthesizes interstitialcellstimulating hormone (ICSH) acceptor; Promote the aromatizing enzyme system activation in follicular cell hyperplasia and granulosa cell, make androgen conversion be oestrogenic hormon; Promote liquor folliculi secretion, follicular cell is increased gradually.The Infertility that Urofollitropin causes mainly for ovulation failure, and be widely used in the Assisted Reproductive Technology ART such as artificial insemination, tube baby.To women, short corpus luteum growth element (LH) can promote that corpus luteum generates, secretion progestogen, has the function of the mesenchymal cell stimulated in testis, secrete androgen to the male sex.
Chorionic gonadotrophin (Human Chorionic Gonadotrophin, HCG) is the refined sugar albumen gonad-stimulating hormone extracted from pregnant woman's urine, is a kind of glycoprotein hormones of placenta syncytiotrophoblast secretion after the mankind become pregnant.Increase rapidly in the concentration of First Trimester hCG, detect the hCG in urine and susceptible of proof gestation.Clinically for Infertility, inadequate luteal function, dysfunctional uterine hemorrhage, cryptorchidism, male hypogonadism, threatened abortion or habitual abortion etc.
At present, the extraction process more complicated of Urofollitropin and chorionic gonadotrophin, the production cycle is long, and product yield is not high, causes production cost greatly to increase, and cannot meet the need of market.Yin little Wei etc. are disclose in the Chinese invention patent of CN101555278A " a kind of extracting method of Urofollitropin " at publication number; Zhao Guiji etc. are also disclose in the Chinese invention patent application of CN102311494A " a kind of method of extraction of human chorionic gonadotropin " at publication number; Extraction process disclosed in this two pieces patent application, compared with prior art, has that Technology is simple, product yield advantages of higher, but also also exists that product foreign matter content is high, purity is low, the shortcoming such as low of tiring, and causes follow-up refining process steps loaded down with trivial details.
Summary of the invention
For the problems referred to above, the object of the present invention is to provide a kind of can significantly Simplified flowsheet, shorten the production time, reduce power consumption, improve Product Activity, yield, purity, a kind of high method extracting gonad-stimulating hormone from people's urine of tiring.
For achieving the above object, technical scheme proposed by the invention is: a kind of method extracting gonad-stimulating hormone from people's urine, is characterized in that, comprise the steps:
Step 1 adjust ph separates out impurity: pH value to 8.0 ~ 9.0 regulating stock liquid with alkali;
Step 2 filtering and impurity removing, ultrafiltration and concentration: after adjust ph, add diatomite in stock liquid or kaolin filters, and then filtrate concentrated by the ultra-filtration membrane that molecular weight cut-off is 8000-15000, obtain ultrafiltration and concentration liquid;
Step 3 ion-exchange chromatography: CM Sepharose Fast Flow ion exchange column on ultrafiltration and concentration liquid, first washs with the NaAc of pH 4.0-5.0, after carry out wash-out with the solution containing 0.3M NaCl, collect elutriant;
Step 4 removing impurity by means of precipitation: under agitation, first in elutriant, add 10% ammonium acetate, and organic solvent makes organic solvent concentration reach 50-60%, centrifugal, collects clear liquid;
Step 5 precipitates with dry: in clear liquid, add organic solvent, make organic solvent concentration reach 80-85%, staticly settle 5-8 hour after stirring, centrifugal collecting precipitate, organic solvent washing 2-3 time, can obtain gonad-stimulating hormone crude product after dehydrating.
Further, described gonad-stimulating hormone is Menotropins, and described stock liquid is menopausal women urine.
Further, described gonad-stimulating hormone is chorionic-gonadotropin hormone, and described stock liquid is pregnancy women urine.
Preferably, described organic solvent is ethanol or acetone.
Preferably, the molecular weight cut-off of the ultra-filtration membrane described in step 2 is 10000.
Adopt technique scheme, the method extracting gonad-stimulating hormone from people's urine of the present invention, has Technology simple, product yield is high, production cost is low, good product purity, advantages of higher of tiring, and the production for subsequent extracted high purity product eliminates many steps.
Embodiment
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1, the extraction of Menotropins, comprises the steps:
(1) by gathering menopausal women urine 1000L, dropping in tank through urine after the assay was approved, regulating pH value to 8.0 ~ 9.0 of urine with alkali;
(2), after adjust ph, in urine, add 10kg diatomite, stir, filter with sheet frame;
(3) solution filtered is squeezed into ultrafiltration tank, be the ultrafiltration membrance filter of 10000 by point sub-aperture, concentrated solution 10L squeezes into setting tank;
(4) pre-install CM Sepharose Fast Flow filler in ion exchange column after, with 2 times of column volume pH 4.5 NaAc buffer A balances, after concentrated solution upper prop, wash with 2 times of column volumes of buffer A again, then with the buffer B wash-out of 2 times of column volumes containing 0.3M NaCl, elutriant is collected;
(5) under agitation, in elutriant, add ethanol make concentration reach 55%, centrifugal, collect clear liquid;
(6) in clear liquid, add ethanol, make concentration reach 80%, staticly settle 5 hours after stirring, throw out absolute ethanol washing 3 times, obtains Menotropins product 0.65g gram after dehydrating.
Use female young big white mouse ovarian weight augmentation method to carry out biological assay, in the Menotropins that aforesaid method is produced, Urofollitropin biological activity is 62.4 international unit/milligram, and interstitialcellstimulating hormone (ICSH) is 71.5 international unit/milligram.
Embodiment 2, the extraction of chorionic-gonadotropin hormone, comprises the steps:
(1) use pregnancy women urine raw material 1000L, will pump in tank through urine after the assay was approved, regulate pH value to 8.0 ~ 9.0 of urine with alkali;
(2) after adjust ph, in urine, add 10kg diatomite or kaolin, stir 30 minutes to evenly, filter with sheet frame;
(3) the solution pump excess of imports filter tank will filtered is the ultrafiltration membrance filter of 10000 by molecular weight cut-off, and the ultrafiltration and concentration liquid 10L obtained squeezes into setting tank;
(4) pre-install Sepharose Fast Flow filler in ion exchange column after, with 2 times of column volume pH 4.5 NaAc buffer A balances, after concentrated solution upper prop, then wash with 2 times of column volumes of buffer A, then with the buffer B wash-out of 2 times of column volumes containing 0.3M NaCl, elutriant is collected;
(5) under agitation, first in elutriant, add 10% ammonium acetate, and organic solvent makes organic solvent concentration reach 60%, centrifugal, collect clear liquid;
(6) in clear liquid, add organic solvent, make organic solvent concentration reach 85%, staticly settle 5 hours after stirring, centrifugal collecting precipitate, organic solvent washing 2-3 time, chorionic-gonadotropin hormone 4.3 grams after dehydrating, can be obtained.
Use female young big white mouse ovarian weight augmentation method to carry out biological assay, the chorionic-gonadotropin hormone biological activity that aforesaid method is produced is 2158 international unit/milligram.
Although specifically show in conjunction with preferred embodiment and describe the present invention; but those skilled in the art should be understood that; not departing from the spirit and scope of the present invention that appended claims limits; in the form and details the present invention is made a variety of changes, be protection scope of the present invention.
Claims (5)
1. from people's urine, extract a method for gonad-stimulating hormone, it is characterized in that, comprise the steps:
Step 1 adjust ph separates out impurity: pH value to 8.0 ~ 9.0 regulating stock liquid with alkali;
Step 2 filtering and impurity removing, ultrafiltration and concentration: after adjust ph, add diatomite in stock liquid or kaolin filters, and then filtrate concentrated by the ultra-filtration membrane that molecular weight cut-off is 8000-15000, obtain ultrafiltration and concentration liquid;
Step 3 ion-exchange chromatography: CM Sepharose Fast Flow ion exchange column on ultrafiltration and concentration liquid, first washs with the NaAc of pH 4.0-5.0, after carry out wash-out with the solution containing 0.3M NaCl, collect elutriant;
Step 4 removing impurity by means of precipitation: under agitation, first in elutriant, add 10% ammonium acetate, and organic solvent makes organic solvent concentration reach 50-60%, centrifugal, collects clear liquid;
Step 5 precipitates with dry: in clear liquid, add organic solvent, make organic solvent concentration reach 80-85%, staticly settle 5-8 hour after stirring, centrifugal collecting precipitate, organic solvent washing 2-3 time, can obtain gonad-stimulating hormone crude product after dehydrating.
2. a kind of method extracting gonad-stimulating hormone from people's urine according to claim 1, it is characterized in that, described gonad-stimulating hormone is Menotropins, and described stock liquid is menopausal women urine.
3. a kind of method extracting gonad-stimulating hormone from people's urine according to claim 1, it is characterized in that, described gonad-stimulating hormone is chorionic-gonadotropin hormone, and described stock liquid is pregnancy women urine.
4. a kind of method extracting gonad-stimulating hormone from people's urine according to the arbitrary claim of claim 1-3, it is characterized in that, described organic solvent is ethanol or acetone.
5. a kind of method extracting gonad-stimulating hormone from people's urine according to the arbitrary claim of claim 1-3, it is characterized in that, the molecular weight cut-off of the ultra-filtration membrane described in step 2 is 10000.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410362136.7A CN104961821A (en) | 2014-07-28 | 2014-07-28 | Method for extracting gonadotrophic hormone from human urine |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410362136.7A CN104961821A (en) | 2014-07-28 | 2014-07-28 | Method for extracting gonadotrophic hormone from human urine |
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| CN104961821A true CN104961821A (en) | 2015-10-07 |
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| CN201410362136.7A Pending CN104961821A (en) | 2014-07-28 | 2014-07-28 | Method for extracting gonadotrophic hormone from human urine |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105732797A (en) * | 2016-04-18 | 2016-07-06 | 山东众山生物科技有限公司 | Purification method for urinary gonadotropin |
| CN106866812A (en) * | 2017-02-27 | 2017-06-20 | 日照岚山生化制品有限公司 | A kind of method that various Urine proteins are extracted in the urine from women |
| CN114805542A (en) * | 2022-04-29 | 2022-07-29 | 江苏尤里卡生物科技有限公司 | Purification method based on gonadotropin in urine |
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| CN1890265A (en) * | 2003-12-22 | 2007-01-03 | 阿雷斯贸易股份有限公司 | Method for purifying fsh |
| CN101087805A (en) * | 2004-11-09 | 2007-12-12 | 阿雷斯贸易股份有限公司 | Method for purifying FSH |
| CN101555278A (en) * | 2009-05-09 | 2009-10-14 | 山东众山生物科技有限公司 | Extraction method of urofollitropin |
| CN102311494A (en) * | 2011-08-12 | 2012-01-11 | 山东众山生物科技有限公司 | Method for extracting human chorionic gonadotropin (HCG) |
| CN102485752A (en) * | 2010-12-01 | 2012-06-06 | 上海丽珠制药有限公司 | Purification method for human chorionic gonadotrophin (HCG) |
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2014
- 2014-07-28 CN CN201410362136.7A patent/CN104961821A/en active Pending
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| CN1890265A (en) * | 2003-12-22 | 2007-01-03 | 阿雷斯贸易股份有限公司 | Method for purifying fsh |
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| CN101555278A (en) * | 2009-05-09 | 2009-10-14 | 山东众山生物科技有限公司 | Extraction method of urofollitropin |
| CN102485752A (en) * | 2010-12-01 | 2012-06-06 | 上海丽珠制药有限公司 | Purification method for human chorionic gonadotrophin (HCG) |
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Non-Patent Citations (1)
| Title |
|---|
| 郭葆玉: "《生物技术制药》", 30 September 2011, 清华大学出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105732797A (en) * | 2016-04-18 | 2016-07-06 | 山东众山生物科技有限公司 | Purification method for urinary gonadotropin |
| CN105732797B (en) * | 2016-04-18 | 2019-07-02 | 山东众山生物科技有限公司 | A kind of purification process of urinary gonadotropin |
| CN106866812A (en) * | 2017-02-27 | 2017-06-20 | 日照岚山生化制品有限公司 | A kind of method that various Urine proteins are extracted in the urine from women |
| CN114805542A (en) * | 2022-04-29 | 2022-07-29 | 江苏尤里卡生物科技有限公司 | Purification method based on gonadotropin in urine |
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Application publication date: 20151007 |