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CN104961582A - High-protein efficient clitocybe maxima culture medium prepared from water-chestnut residues and preparation method for high-protein efficient clitocybe maxima culture medium - Google Patents

High-protein efficient clitocybe maxima culture medium prepared from water-chestnut residues and preparation method for high-protein efficient clitocybe maxima culture medium Download PDF

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Publication number
CN104961582A
CN104961582A CN201510385411.1A CN201510385411A CN104961582A CN 104961582 A CN104961582 A CN 104961582A CN 201510385411 A CN201510385411 A CN 201510385411A CN 104961582 A CN104961582 A CN 104961582A
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parts
water
culture medium
clitocybe maxima
subsequent use
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CN201510385411.1A
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Inventor
柯家厚
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Hefei Fuquan Modern Agriculture Science And Technology Ltd
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Hefei Fuquan Modern Agriculture Science And Technology Ltd
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Abstract

The invention discloses high-protein efficient clitocybe maxima culture medium prepared from water-chestnut residues. The high-protein efficient clitocybe maxima culture medium is characterized by comprising the following components in parts by weight: 30 to 40 parts of water chestnut residues, 20 to 30 parts of cotton seed hulls, 10 to 20 parts of rice chaff, 3 to 5 parts of montmorillonite powder, 2 to 3 parts of diatomite, 0.01 to 0.03 parts of trichoderma viride, 0.03 to 0.05 parts of aspergillus niger, 0.03 to 0.05 parts of geotrichum candidum, 0.1 to 0.3 parts of Candida lambica, 0.4 to 0.6 parts of bacillus and 0.3 to 0.5 parts of pediococcus acidilactici. The high-protein efficient clitocybe maxima culture medium adopts enzyme carried on montmorillonite and activated zymophyte carried and loaded on diatomite to respectively degrade and ferment insoluble organic substances, so that the degradation and fermentation speeds are effectively regulated, and the formation of an intermediate product is promoted; and finally, by virtue of high-temperature stirring reaction, reaction of the intermediate product is promoted to form high-activity biochemical humic acid, so that the absorption and the metabolism of clitocybe maxima are convenient, and efficient cultivation of the clitocybe maxima is realized.

Description

A kind of adopt water chestnut slag to prepare clitocybe maxima high protein efficient culture medium and preparation method
Technical field
The invention belongs to breed of edible fungus field, be specifically related to a kind of adopt water chestnut slag to prepare clitocybe maxima high protein efficient culture medium and preparation method.
Background technology
Edible mushrooms has abundant nutrition and good nourishing function because of it, enjoys the parent of people to look at.In recent years, edible mushrooms market scale constantly expands, and in order to meet the need of market, China's Edible Fungi is just towards the future development of industrialization, mass-producing and batch production.The edible fungus culturing fertilizer of green high-efficient has become an urgent demand of edible mushrooms development.Meanwhile, also to there is raw material availability low for Edible Fungi.Output needs the problem improved further.
Biochemical humic acid is by chemistry with microorganism fermentation process makes is a kind of organism by agricultural crop straw, wood chip etc., except containing except active xanthohumic acid, amino acid also containing some amount and abundant carboxyl, hydroxyl, phenolic hydroxyl group, quinone hydroxyl isoreactivity group, compared with mineral humic acid, have the following advantages: 1, molecular weight is little, seepage force is strong, more easily absorbed by crops and utilization; 2, active group rich content, physiologically active and chemically reactive stronger; 3, composition variation, active high, effect is more excellent.
Summary of the invention
The present invention is directed to the artificial culture demand of edible mushrooms, propose a kind of adopt water chestnut slag to prepare clitocybe maxima high protein efficient culture medium and preparation method.
The technical solution used in the present invention is as follows:
A kind of clitocybe maxima high protein efficient culture medium adopting water chestnut slag to prepare, it is characterized in that, comprise following parts by weight of component: water chestnut slag 30-40, cotton seed hulls 20-30, rice chaff 10-20, unslaked lime 2-4, vegetable seed straw powder 10-20, wheat bran 10-20, calcium carbonate 2-3, terra alba 5-7, Zein powder 10-20, montmorillonite powder 3-5, diatomite 2-3, hawthorn powder 15-20, dry rabbit excrement 3-5, sweet potato waste 10-20, chrysanthemum slag 12-14, viride 0.01-0.03, aspergillus niger 0.03-0.05, geotrichum candidum 0.03-0.05, the comparable candiyeast 0.1-0.3 of youth, genus bacillus 0.4-0.6, pediococcus acidilactici 0.3-0.5, urea 1-2, potassium primary phosphate 0.6-0.8, magnesium chloride 0.5-0.7, organic chelated leaven promoting agent 1-2, appropriate water.
The clitocybe maxima high protein efficient culture medium preparation method adopting water chestnut slag to prepare, is characterized in that, comprise the following steps:
(1) get water chestnut slag, cotton seed hulls, the mixing of vegetable seed straw powder, after adopting unslaked lime 100 times of water diluents to soak 2-3h, adopt High Temperature High Pressure decompressor process 2-3min, obtain expansion activated camplex for subsequent use;
(2) get montmorillonite powder, Zein powder, viride, aspergillus niger, geotrichum candidum and gross weight 1-2 water mix and blend 5-10min doubly to evenly, leave standstill 4-6h, obtaining montmorillonite powder absorption, to carry enzyme for subsequent use; Get diatomite, wheat bran, the comparable candiyeast of youth, genus bacillus, pediococcus acidilactici and gross weight 1-2 water mix and blend 6-8min doubly to evenly, leave standstill 2-3h, obtain the activation zymophyte that diatomite adsorption carries for subsequent use;
(3) get expansion activated camplex, enzyme mixing is carried in montmorillonite powder absorption, and add gross weight 3-5 water doubly, airtight stirring 20-30min is extremely even, airtight placement 2-3d at 38-42 DEG C afterwards, and the activation degraded mixture that must expand is for subsequent use;
(4) get rice chaff, activation zymophyte mixing that hawthorn powder, dry rabbit excrement, sweet potato waste, chrysanthemum slag, diatomite adsorption carry, and after adding gross weight 4-8 water mixing doubly, sealing and fermenting 20-30d, pumping into afterwards expands activates degraded mixture and stirs, leave standstill 2-3d, 80-90 DEG C of stirring reaction 30-50min in closed environment, cooling, obtains biochemical humic acid mixture for subsequent use;
(5) get calcium carbonate, terra alba, urea, potassium primary phosphate, magnesium chloride, organic chelated leaven promoting agent and gross weight 2-3 water doubly to mix to evenly, obtain nutritive medium for subsequent use;
(6) get biochemical humic acid mixture, nutritive medium mixing and stirring, and adjust the 50-60% that moisture content is gross weight, obtain culture material for subsequent use;
(7) be divided in polyethylene plastic bag by culture material, put into the steamer of band high-pressure valve and cooling valve afterwards, first vigorous fire is warming up to 100 DEG C, sterilizing 1-2h, being cooled to rapidly 0 DEG C by cooling valve, is warming up to 100 DEG C at secondary vigorous fire, sterilizing 30-50min, is cooled to room temperature, to obtain final product.
Beneficial effect of the present invention is embodied in:
The activation zymophyte that the enzyme that the present invention adopts polynite to carry and diatomite carry loading carries out organic degraded hard to tolerate and fermentation respectively, effectively have adjusted degraded and fermenting speed, facilitate the formation of intermediate product, finally reacted by high-temperature stirring, facilitate the reaction of intermediate product, form highly active biochemical humic acid, be convenient to absorption and the metabolism of clitocybe maxima, realize the Efficient Cultivation of clitocybe maxima.Avoid the intermediate product dullness that simple cellulase degradation causes, can not provide for final product the defect and deficiency enriching source.
Embodiment
Embodiment
Clitocybe maxima high protein efficient culture medium prepared by the employing water chestnut slag described in the present embodiment, it is characterized in that, comprise following parts by weight of component: water chestnut slag 35, cotton seed hulls 25, rice chaff 15, unslaked lime 3, vegetable seed straw powder 15, wheat bran 15, calcium carbonate 2.5, terra alba 6, Zein powder 15, montmorillonite powder 4, diatomite 2.5, hawthorn powder 17.5, dry rabbit excrement 4, sweet potato waste 15, chrysanthemum slag 13, viride 0.02, aspergillus niger 0.04, geotrichum candidum 0.04, the comparable candiyeast 0.2 of youth, genus bacillus 0.5, pediococcus acidilactici 0.4, urea 1.5, potassium primary phosphate 0.7, magnesium chloride 0.6, organic chelated leaven promoting agent 1.5, appropriate water.
Clitocybe maxima high protein efficient culture medium preparation method prepared by the employing water chestnut slag described in the present embodiment, is characterized in that, comprise the following steps:
(1) get water chestnut slag, cotton seed hulls, the mixing of vegetable seed straw powder, after adopting unslaked lime 100 times of water diluents to soak 2-3h, adopt High Temperature High Pressure decompressor process 2-3min, obtain expansion activated camplex for subsequent use;
(2) get montmorillonite powder, Zein powder, viride, aspergillus niger, geotrichum candidum and gross weight 1-2 water mix and blend 5-10min doubly to evenly, leave standstill 4-6h, obtaining montmorillonite powder absorption, to carry enzyme for subsequent use; Get diatomite, wheat bran, the comparable candiyeast of youth, genus bacillus, pediococcus acidilactici and gross weight 1-2 water mix and blend 6-8min doubly to evenly, leave standstill 2-3h, obtain the activation zymophyte that diatomite adsorption carries for subsequent use;
(3) get expansion activated camplex, enzyme mixing is carried in montmorillonite powder absorption, and add gross weight 3-5 water doubly, airtight stirring 20-30min is extremely even, airtight placement 2-3d at 38-42 DEG C afterwards, and the activation degraded mixture that must expand is for subsequent use;
(4) get rice chaff, activation zymophyte mixing that hawthorn powder, dry rabbit excrement, sweet potato waste, chrysanthemum slag, diatomite adsorption carry, and after adding gross weight 4-8 water mixing doubly, sealing and fermenting 20-30d, pumping into afterwards expands activates degraded mixture and stirs, leave standstill 2-3d, 80-90 DEG C of stirring reaction 30-50min in closed environment, cooling, obtains biochemical humic acid mixture for subsequent use;
(5) get calcium carbonate, terra alba, urea, potassium primary phosphate, magnesium chloride, organic chelated leaven promoting agent and gross weight 2-3 water doubly to mix to evenly, obtain nutritive medium for subsequent use;
(6) get biochemical humic acid mixture, nutritive medium mixing and stirring, and adjust the 50-60% that moisture content is gross weight, obtain culture material for subsequent use;
(7) be divided in polyethylene plastic bag by culture material, put into the steamer of band high-pressure valve and cooling valve afterwards, first vigorous fire is warming up to 100 DEG C, sterilizing 1-2h, being cooled to rapidly 0 DEG C by cooling valve, is warming up to 100 DEG C at secondary vigorous fire, sterilizing 30-50min, is cooled to room temperature, to obtain final product.

Claims (2)

1. the clitocybe maxima high protein efficient culture medium adopting water chestnut slag to prepare, it is characterized in that, comprise following parts by weight of component: water chestnut slag 30-40, cotton seed hulls 20-30, rice chaff 10-20, unslaked lime 2-4, vegetable seed straw powder 10-20, wheat bran 10-20, calcium carbonate 2-3, terra alba 5-7, Zein powder 10-20, montmorillonite powder 3-5, diatomite 2-3, hawthorn powder 15-20, dry rabbit excrement 3-5, sweet potato waste 10-20, chrysanthemum slag 12-14, viride 0.01-0.03, aspergillus niger 0.03-0.05, geotrichum candidum 0.03-0.05, the comparable candiyeast 0.1-0.3 of youth, genus bacillus 0.4-0.6, pediococcus acidilactici 0.3-0.5, urea 1-2, potassium primary phosphate 0.6-0.8, magnesium chloride 0.5-0.7, organic chelated leaven promoting agent 1-2, appropriate water.
2. the clitocybe maxima high protein efficient culture medium preparation method adopting water chestnut slag to prepare, is characterized in that, comprise the following steps:
(1) get water chestnut slag, cotton seed hulls, the mixing of vegetable seed straw powder, after adopting unslaked lime 100 times of water diluents to soak 2-3h, adopt High Temperature High Pressure decompressor process 2-3min, obtain expansion activated camplex for subsequent use;
(2) get montmorillonite powder, Zein powder, viride, aspergillus niger, geotrichum candidum and gross weight 1-2 water mix and blend 5-10min doubly to evenly, leave standstill 4-6h, obtaining montmorillonite powder absorption, to carry enzyme for subsequent use; Get diatomite, wheat bran, the comparable candiyeast of youth, genus bacillus, pediococcus acidilactici and gross weight 1-2 water mix and blend 6-8min doubly to evenly, leave standstill 2-3h, obtain the activation zymophyte that diatomite adsorption carries for subsequent use;
(3) get expansion activated camplex, enzyme mixing is carried in montmorillonite powder absorption, and add gross weight 3-5 water doubly, airtight stirring 20-30min is extremely even, airtight placement 2-3d at 38-42 DEG C afterwards, and the activation degraded mixture that must expand is for subsequent use;
(4) get rice chaff, activation zymophyte mixing that hawthorn powder, dry rabbit excrement, sweet potato waste, chrysanthemum slag, diatomite adsorption carry, and after adding gross weight 4-8 water mixing doubly, sealing and fermenting 20-30d, pumping into afterwards expands activates degraded mixture and stirs, leave standstill 2-3d, 80-90 DEG C of stirring reaction 30-50min in closed environment, cooling, obtains biochemical humic acid mixture for subsequent use;
(5) get calcium carbonate, terra alba, urea, potassium primary phosphate, magnesium chloride, organic chelated leaven promoting agent and gross weight 2-3 water doubly to mix to evenly, obtain nutritive medium for subsequent use;
(6) get biochemical humic acid mixture, nutritive medium mixing and stirring, and adjust the 50-60% that moisture content is gross weight, obtain culture material for subsequent use;
(7) be divided in polyethylene plastic bag by culture material, put into the steamer of band high-pressure valve and cooling valve afterwards, first vigorous fire is warming up to 100 DEG C, sterilizing 1-2h, being cooled to rapidly 0 DEG C by cooling valve, is warming up to 100 DEG C at secondary vigorous fire, sterilizing 30-50min, is cooled to room temperature, to obtain final product.
CN201510385411.1A 2015-07-06 2015-07-06 High-protein efficient clitocybe maxima culture medium prepared from water-chestnut residues and preparation method for high-protein efficient clitocybe maxima culture medium Pending CN104961582A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104016776A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizigus marmoreus culture medium prepared from chestnut shell and preparation method thereof
CN104030777A (en) * 2014-05-07 2014-09-10 合肥福泉现代农业科技有限公司 Needle mushroom medium with peanut shell as raw material, and preparation method thereof
CN104256057A (en) * 2014-10-01 2015-01-07 青岛嘉瑞生物技术有限公司 Method for preparing feed proteins by utilizing alcohol waste liquor and crop straws

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104016776A (en) * 2014-05-07 2014-09-03 合肥福泉现代农业科技有限公司 Hypsizigus marmoreus culture medium prepared from chestnut shell and preparation method thereof
CN104030777A (en) * 2014-05-07 2014-09-10 合肥福泉现代农业科技有限公司 Needle mushroom medium with peanut shell as raw material, and preparation method thereof
CN104256057A (en) * 2014-10-01 2015-01-07 青岛嘉瑞生物技术有限公司 Method for preparing feed proteins by utilizing alcohol waste liquor and crop straws

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