CN104928331A - Technology for preparing functional xylo-oligosaccharide by comprehensively utilizing wheat straw - Google Patents
Technology for preparing functional xylo-oligosaccharide by comprehensively utilizing wheat straw Download PDFInfo
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- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 title claims abstract description 29
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for extracting xylo-oligosaccharide from wheat straw. The wheat straw, an agricultural and sideline product, is used as a raw material, and a microbial fermentation technology and a biological enzyme technology are utilized for preparing xylo-oligosaccharide which has the functions of promoting bifidobacterium to proliferate, preventing constipation, lowering the content of cholesterol, protecting the liver and the like. The quality of the obtained xylo-oligosaccharide product meets the xylo-oligosaccharide industrial standard request, efficient utilization of the straw is achieved, the utilization value of the straw is increased, and a good popularization prospect is achieved. The method for extracting the xylo-oligosaccharide from the wheat straw has the advantages that the preparation technology combining compound microorganism solid fermentation and ultrasonic-assisted enzymolysis is adopted, so that the yield of the xylo-oligosaccharide is increased; ultrasonic-assisted enzymolysis is adopted for removal of proteins and starch and enzymolysis of phytase and mannose, and therefore the purity of the xylo-oligosaccharide product is remarkably improved; multi-bacteria mixed fermentation is adopted, so that the capacity of fermentation and enzymolysis of xylan is improved; the production technology is simple, energy consumption is low, environmental pollution is avoided, the investment is small, and mass production is easy to conduct.
Description
Technical field
The present invention relates to a kind of technique fully utilizing wheat stalk and prepare functional low polyxylose, belong to agricultural byproducts technical field of comprehensive utilization.
Background technology
Along with the development of society and improving constantly of people's living standard, people have also had larger lifting to the degree of concern of health thereupon, and the appearance of functional foodstuff has started one new research and development upsurge.Wherein, functional oligose as a kind of functional food ingredient, have promote bifidus bacillus propagation, regulating intestinal canal flora, suppress intracellular toxin, protection liver function, regulate intestines and stomach function, anti-ly treat constipation and suffer from diarrhoea, activate immunity, anti-ageing, antitumor, reduce serum cholesterol, hypotensive, low-yield or noenergy, can not cause the uniquenesses such as carious tooth physiological function and by whole world extensive concern.Xylo-oligosaccharide is as the higher functional oligose of a kind of added value, and its Selection utilization and the proliferation function to bifidus bacillus are all better than other functional oligoses.For the research and development of xylo-oligosaccharide, the history in Japan's existing more than 20 years so far, substantially realize industrialization at present, European Countries also competitively heats up to its exploitation.The development research of China to xylo-oligosaccharide starts from the nineties in last century, and the starting material prepared for xylo-oligosaccharide at present mainly concentrate on corn cob.
Wheat stalk is ripe wheat stalk, leaf, fringe part summation, is remainder after wheat maturation acquisition seed, accounts for crop quality 50%.China produces nearly 1.1 hundred million tons of wheat stalk every year, except minority is by as except animal feed, farm manure and rural area fuel, major part is stacked or also field after directly burning, and effective industrial utilization proportion is extremely low, waste precious resources, and very big pollution is also caused to environment.Modern cereal science research finds, and wheat stalk contains and multiplely has antitumor, anti-oxidant important physiologically active substance, as food fibre, araboxylan, Dui – coumaric acid and forulic acid; Wherein, in recent years general concern has been caused to the research of xylo-oligosaccharide both at home and abroad.Straw is utilized to mainly contain NaOH extraction, acid system dry blowing and alkali lye lixiviate combined techniques, KOH extraction, H for xylo-oligosaccharide at present
2o
2with alkali lye lixiviate combined techniques, biologic enzymolysis method etc., but there is the drawbacks such as complex process, environmental pollution is serious, xylan yield is low, xylanase activity is low, therefore the technique of traditional preparation of xylooligosaccharideswith with enzyme is simplified, for the preparation of xylo-oligosaccharide provides new way, can also be fully utilized agricultural byproducts wheat stalk, extend agricultural byproducts processing chain, stalk is turned waste into wealth, there is very important social effect and economic worth.
Summary of the invention
Pin the problems referred to above of the present invention, utilize fermentable and biological enzymolysis technology, provide a kind of technique fully utilizing wheat stalk and prepare functional low polyxylose.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
The invention provides a kind of technique fully utilizing wheat stalk and prepare functional low polyxylose, it is characterized in that specifically comprising the following steps:
(1) pulverize: choosing the stalk without going mouldy, pulverizing 100 mesh sieves;
(2) boiling: boiling 0.5 ~ 1h under 150 DEG C of conditions, after cooling, adjustment stalk and quality are than being 1:8 ~ 10;
(3)) ultrasound-assisted enzymolysis destarching: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 3 ~ 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 3 ~ 5 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis Deproteinization: pH to 8.0 ~ 9.0 of adjustment suspension, Sumizyme MP is added in the ratio of wheat stalk mass ratio 2 ~ 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 60 ~ 90min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyte, adds the distilled water flushing 2 ~ 3 times of 3 ~ 5 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 8 ~ 10 parts of zeolites, 3 ~ 5 parts of monocalcium phosphates, 6 ~ 8 parts of glucose, 10 ~ 15 parts of rice brans, 10 ~ 15 portions of Semen Maydis powder, 3 ~ 5 parts of rapeseed cakes, 10 ~ 15 parts of wheat stalks, 2 ~ 3 parts of Trichodermareeseis, 1 ~ 2 part of aspergillus niger, 1 ~ 2 part of yeast, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobically fermenting 3 ~ 5d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 3 ~ 5 parts, cellulase 2 ~ 3 parts, zytase 2 ~ 3 parts of ratios mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobically fermenting: by mixed mixture temperature control anaerobically fermenting 2 ~ 3d, temperature 28 ~ 32 DEG C, periodic agitation; The pure water of substratum 3 ~ 5 times of mass ratioes is added after fermentation ends;
(8) ultrasonic wave secondary assistance enzymolysis: add stalk mass ratio 1 ~ 2% zytase in fermentation water lotion, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 30 ~ 60min;
(9) centrifugal: centrifugal supernatant liquor and solid residue; 1 ~ 2 times of pure water is added in solid residue, centrifugal after stirring, merge supernatant liquor;
(10) ferment treatment: supernatant liquor is regulated pH5.0 ~ 5.5, adds 0.2 ~ 0.5% phytase, 0.3 ~ 0.6% mannase, enzymolysis 30 ~ 60min under 40 ~ 50 DEG C of conditions, and go out after enzymolysis terminates enzyme;
(11) alcohol is analysed: 95% ethanol progressively adding equal-volume ratio in solution precipitates;
(12) pulverizing is dried: the precipitation of acquisition dried after pulverizing and namely obtain wheat stalk xylan product.
The invention provides a kind of technique fully utilizing wheat stalk and prepare functional low polyxylose, it is advantageous that: the preparation technology that (1) is combined by complex microorganism solid fermentation and ultrasound-assisted enzymolysis, improve the yield of xylo-oligosaccharide, every 100g stalk xylo-oligosaccharide output reaches 41.5g, show that a part of Mierocrystalline cellulose generates xylo-oligosaccharide by the degraded of fermentable and cellulase, lifting 45.88% compared with single creature enzymolysis process, the composition analyzing xylo-oligosaccharide is mainly xylo-bioses, xylotriose, Xylotetrose; (2) by the enzymolysis of ultrasound-assisted enzymolysis Deproteinization, starch and phytase, mannase, significantly improve the purity of xylo-oligosaccharide product, reach 97.4%; (3) aspergillus niger and Trichodermareesei are the most significant bacterial strains of current known degradation of fibers effect, the reciprocal symbiosis relation of mould and yeast mixed culture makes microbial reproduction and enzymatic productivity improve a lot, yeast not only can play derepression, and to have the growth of fungi and just imitate, analyze the mould assimilation starches such as reason Trichodermareesei, aspergillus niger and cellulosic ability strong, the structural carbohydrate of stalk can be degraded to the monose materials such as the available monosaccharide and disaccharide of yeast, make yeast be able to good growth; In addition, in mixed fermentation with various bacterium, the Institute of Micro-biology that the sugar that enzymatic action generates is fermented sugar immediately utilizes, so just maintain the concentration of degradation product, what eliminate the degradation product that enzymic synthesis effect is subject to checks effect, also relieves the feedback inhibition of reacting final product to enzyme, shortens fermenting process; (4) adopt solid state fermentation, production technique is simple, and energy consumption is low, non-environmental-pollution, investment are little, be easy to large-scale production.
The invention provides a kind of technique fully utilizing wheat stalk and prepare functional low polyxylose, gained xylo-oligosaccharide product quality reaches xylo-oligosaccharide Industry code requirements, simultaneously, the present invention is using wheat processing by product stalk as the extraction raw material of xylo-oligosaccharide product, achieve the efficiency utilization of stalk, improve the utility value of stalk, there is good promotion prospect.
Embodiment
embodiment 1:
Fully utilize the technique that wheat stalk prepares functional low polyxylose, concrete preparation process is as follows:
(1) pulverize: choosing the stalk without going mouldy, pulverizing 100 mesh sieves;
(2) boiling: boiling 0.5h under 150 DEG C of conditions, after cooling, adjustment stalk and quality are than being 1:10;
(3)) ultrasound-assisted enzymolysis destarching: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 5 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis Deproteinization: pH to 8.0 ~ 9.0 of adjustment suspension, Sumizyme MP is added in the ratio of wheat stalk mass ratio 2%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 90min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyte, adds the distilled water flushing 3 times of 3 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 8 parts of zeolites, 5 parts of monocalcium phosphates, 6 parts of glucose, 15 parts of rice brans, 10 portions of Semen Maydis powder, 5 parts of rapeseed cakes, 10 parts of wheat stalks, 2 parts of Trichodermareeseis, 1 part of aspergillus niger, 1 part of yeast, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobically fermenting 5d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 3 parts, cellulase 2.5 parts, zytase 2 parts of ratios mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobically fermenting: by mixed mixture temperature control anaerobically fermenting 3d, temperature 28 ~ 32 DEG C, periodic agitation; The pure water of substratum 5 times of mass ratioes is added after fermentation ends;
(8) ultrasonic wave secondary assistance enzymolysis: add stalk mass ratio 1% zytase, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W in fermentation water lotion, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 60min;
(9) centrifugal: centrifugal supernatant liquor and solid residue; 1 times of pure water is added in solid residue, centrifugal after stirring, merge supernatant liquor;
(10) ferment treatment: supernatant liquor is regulated pH5.0 ~ 5.5, adds 0.2% phytase, 0.3% mannase, enzymolysis 60min under 40 ~ 50 DEG C of conditions, and go out after enzymolysis terminates enzyme;
(11) alcohol is analysed: 95% ethanol progressively adding equal-volume ratio in solution precipitates;
(12) pulverizing is dried: the precipitation of acquisition dried after pulverizing and namely obtain wheat stalk xylan product.
embodiment 2:
Fully utilize the technique that wheat stalk prepares functional low polyxylose, concrete preparation process is as follows:
(1) pulverize: choosing the stalk without going mouldy, pulverizing 100 mesh sieves;
(2) boiling: boiling 0.8h under 150 DEG C of conditions, after cooling, adjustment stalk and quality are than being 1:9;
(3)) ultrasound-assisted enzymolysis destarching: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 4%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 4 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis Deproteinization: pH to 8.0 ~ 9.0 of adjustment suspension, Sumizyme MP is added in the ratio of wheat stalk mass ratio 2.5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 75min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyte, adds the distilled water flushing 2 times of 4 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 9 parts of zeolites, 4 parts of monocalcium phosphates, 7 parts of glucose, 13 parts of rice brans, 12 portions of Semen Maydis powder, 4 parts of rapeseed cakes, 12 parts of wheat stalks, 2.5 parts of Trichodermareeseis, 1.5 parts of aspergillus nigers, 1.5 parts of yeast, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobically fermenting 4d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 4 parts, cellulase 2 parts, zytase 2.5 parts of ratios mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobically fermenting: by mixed mixture temperature control anaerobically fermenting 2.5d, temperature 28 ~ 32 DEG C, periodic agitation; The pure water of substratum 4 times of mass ratioes is added after fermentation ends;
(8) ultrasonic wave secondary assistance enzymolysis: add stalk mass ratio 1.5% zytase, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W in fermentation water lotion, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 45min;
(9) centrifugal: centrifugal supernatant liquor and solid residue; 1.5 times of pure water are added in solid residue, centrifugal after stirring, merge supernatant liquor;
(10) ferment treatment: supernatant liquor is regulated pH5.0 ~ 5.5, adds 0.4% phytase, 0.5% mannase, enzymolysis 45min under 40 ~ 50 DEG C of conditions, and go out after enzymolysis terminates enzyme;
(11) alcohol is analysed: 95% ethanol progressively adding equal-volume ratio in solution precipitates;
(12) pulverizing is dried: the precipitation of acquisition dried after pulverizing and namely obtain wheat stalk xylan product.
embodiment 3:
Fully utilize the technique that wheat stalk prepares functional low polyxylose, concrete preparation process is as follows:
(1) pulverize: choosing the stalk without going mouldy, pulverizing 100 mesh sieves;
(2) boiling: boiling 1h under 150 DEG C of conditions, after cooling, adjustment stalk and quality are than being 1:8;
(3)) ultrasound-assisted enzymolysis destarching: pH to 5.5 ~ 7.5 of adjustment wheat stalk suspension, α ~ amylase is added in the ratio of wheat stalk mass ratio 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 3 times of mass ratioes, obtain suspension in wheat stalk residue, for subsequent use;
(4) ultrasound-assisted enzymolysis Deproteinization: pH to 8.0 ~ 9.0 of adjustment suspension, Sumizyme MP is added in the ratio of wheat stalk mass ratio 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 60min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyte, adds the distilled water flushing 2 times of 5 times of mass ratioes, filter to obtain wheat stalk residue in filter residue;
(5) bio-fermentation agent preparation: get 10 parts of zeolites, 3 parts of monocalcium phosphates, 8 parts of glucose, 10 parts of rice brans, 15 portions of Semen Maydis powder, 3 parts of rapeseed cakes, 15 parts of wheat stalks, 3 parts of Trichodermareeseis, 2 parts of aspergillus nigers, 2 parts of yeast, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobically fermenting 3d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: wheat stalk residue 100 parts, bio-fermentation agent 5 parts, cellulase 3 parts, zytase 3 parts of ratios mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobically fermenting: by mixed mixture temperature control anaerobically fermenting 2d, temperature 28 ~ 32 DEG C, periodic agitation; The pure water of substratum 5 times of mass ratioes is added after fermentation ends;
(8) ultrasonic wave secondary assistance enzymolysis: add stalk mass ratio 2% zytase, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W in fermentation water lotion, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 30min;
(9) centrifugal: centrifugal supernatant liquor and solid residue; 2 times of pure water are added in solid residue, centrifugal after stirring, merge supernatant liquor;
(10) ferment treatment: supernatant liquor is regulated pH5.0 ~ 5.5, adds 0.5% phytase, 0.6% mannase, enzymolysis 30min under 40 ~ 50 DEG C of conditions, and go out after enzymolysis terminates enzyme;
(11) alcohol is analysed: 95% ethanol progressively adding equal-volume ratio in solution precipitates;
(12) pulverizing is dried: the precipitation of acquisition dried after pulverizing and namely obtain wheat stalk xylan product.
The invention provides a kind of technique fully utilizing wheat stalk and prepare functional low polyxylose, the functional of xylo-oligosaccharide is studied by animal experiment, primary study xylo-oligosaccharide is preventing constipation, reduce cholesterol, effect of the aspects such as protection liver, result shows: (1) mice with constipation model trial shows that the xylo-oligosaccharide obtained has function of relaxing bowel, after per os gives the xylo-oligosaccharide of mouse various dose, each dosage group all obviously can increase the Small intestine advance activity of mouse, shorten the defecation time first of mice with constipation, increase defecation grain number and the defecation weight of mice with constipation, and each dosage group is showed no diarrhoea phenomenon in process of the test, illustrate that mouse gives dosage reasonable, the form of ight soil is chocolate particulate state, ight soil is slightly soft compared with control group mice ight soil, surface wettability, the moistening degree of ight soil is moderate, sense organ is normal, (2) by mouse feeding high lipid food, hyperlipemia in mice model is set up, with research experiment.Test-results shows, blank group and model control group blood lipid level difference statistically can determine modeling success.Test group extremely significantly can reduce TC, TG, LDL-c, level (P<0.01) compared with model control group; (3) by the mensuration to hyperlipemia in mice blood glucose value, compared with model control group by blank group, illustrate that mouse blood sugar model is set up, test-results shows, xylo-oligosaccharide has function of blood sugar reduction to mouse.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (1)
1. from Testa Tritici, extract a method for xylo-oligosaccharide, it is characterized in that specifically comprising the following steps:
(1) pulverize: choosing the wheat bran without going mouldy, pulverizing 100 mesh sieves;
(2) boiling: boiling 0.5 ~ 1h under 150 DEG C of conditions, after cooling, adjustment wheat bran and quality are than being 1:8 ~ 10;
(3)) ultrasound-assisted enzymolysis destarching: pH to 5.5 ~ 7.5 of adjustment Testa Tritici suspension, α ~ amylase is added in the ratio of Testa Tritici mass ratio 3 ~ 5%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 70 ~ 80 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and utilizes iodine fluid inspection to starch complete hydrolysis; Enzymolysis terminates rear filtration, and filtrate is starch hydrolyzate, adds the distilled water of 3 ~ 5 times of mass ratioes, obtain suspension in Testa Tritici residue, for subsequent use;
(4) ultrasound-assisted enzymolysis Deproteinization: pH to 8.0 ~ 9.0 of adjustment suspension, Sumizyme MP is added in the ratio of Testa Tritici mass ratio 2 ~ 3%, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 60 ~ 90min; Go out after enzymolysis terminates enzyme, and filter, filtrate is protein hydrolyte, adds the distilled water flushing 2 ~ 3 times of 3 ~ 5 times of mass ratioes, filter to obtain Testa Tritici residue in filter residue;
(5) bio-fermentation agent preparation: get 8 ~ 10 parts of zeolites, 3 ~ 5 parts of monocalcium phosphates, 6 ~ 8 parts of glucose, 10 ~ 15 parts of rice brans, 10 ~ 15 portions of Semen Maydis powder, 3 ~ 5 parts of rapeseed cakes, 10 ~ 15 parts of Testa Triticis, 2 ~ 3 parts of Trichodermareeseis, 1 ~ 2 part of aspergillus niger, 1 ~ 2 part of yeast, mixing and stirring; Then moisture is adjusted to 35 ~ 40%, anaerobically fermenting 3 ~ 5d at 25 DEG C ~ 30 DEG C with the aqueous solution containing 2% molasses;
(6) batch mixing: Testa Tritici residue 100 parts, bio-fermentation agent 3 ~ 5 parts, cellulase 2 ~ 3 parts, zytase 2 ~ 3 parts of ratios mix in mass ratio, adjustment moisture to 35 ~ 40%;
(7) anaerobically fermenting: by mixed mixture temperature control anaerobically fermenting 2 ~ 3d, temperature 28 ~ 32 DEG C, periodic agitation; The pure water of substratum 3 ~ 5 times of mass ratioes is added after fermentation ends;
(8) ultrasonic wave secondary assistance enzymolysis: add wheat bran mass ratio 1 ~ 2% zytase in fermentation water lotion, enzymolysis under ultrasonic assistant effect, hydrolysis temperature 50 ~ 60 DEG C, ultrasonic power 300W, period adopts stirrer at the uniform velocity to stir, and enzymolysis time is 30 ~ 60min;
(9) centrifugal: centrifugal supernatant liquor and solid residue; 1 ~ 2 times of pure water is added in solid residue, centrifugal after stirring, merge supernatant liquor;
(10) ferment treatment: supernatant liquor is regulated pH5.0 ~ 5.5, adds 0.2 ~ 0.5% phytase, 0.3 ~ 0.6% mannase, enzymolysis 30 ~ 60min under 40 ~ 50 DEG C of conditions, and go out after enzymolysis terminates enzyme;
(11) alcohol is analysed: 95% ethanol progressively adding equal-volume ratio in solution precipitates;
(12) pulverizing is dried: the precipitation of acquisition dried after pulverizing and namely obtain Testa Tritici xylan product.
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