CN104921838B - A kind of Mesentery microcirculation observation constant temperature and humidity perfusion device and its application - Google Patents
A kind of Mesentery microcirculation observation constant temperature and humidity perfusion device and its application Download PDFInfo
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- CN104921838B CN104921838B CN201510436733.4A CN201510436733A CN104921838B CN 104921838 B CN104921838 B CN 104921838B CN 201510436733 A CN201510436733 A CN 201510436733A CN 104921838 B CN104921838 B CN 104921838B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
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Abstract
The present invention provides a kind of Mesentery microcirculation observation and uses constant temperature and humidity perfusion device, including perfusion box, fixed plate and temperature control equipment；Present invention simultaneously provides its application in microexamination animal Mesentery microcirculation.Small volume of the present invention, constant temperature and humidity, it is easy to keep mesenterium sample cleaning, experimental animal can be fixedly secured and be fitted close with microscope.
Description
Technical field
The invention belongs to medical microscopic viewing instrument field, and in particular to a kind of Mesentery microcirculation observation constant temperature and humidity
Perfusion device and its application.
Background technology
Microcirculation area is the place that blood and tissue fluid directly carry out mass exchange, and its blood circumstance can be by means of micro-
Mirror is observed.The tissue at mesenterium position is thin, is suitable for directly observing easypro contracting and the blood circumstance of microcirculation area blood vessel.
The constant temperature perfusion device volume of existing Mesentery microcirculation observation is larger, aobvious with the microcirculation sold in the market
Micro mirror matches, and is difficult greatly to lay due to volume, it is impossible in the multinomial projection microscope of common biomicroscope, especially Bu Laide
Used on the high-end microscope such as system, have impact on the fineness of Microcirculatory Observations.The micro-circle microscope sold in the market
Resolution ratio is smaller, unitary function, is mainly used in the gross examination of microcirculation form, fluidised form and surrounding shape body, it is impossible to microcirculation
Cytorheology is carried out deeper into, careful observational study.The multinomial projection microscope system in cloth Rider and part are special biological aobvious
Micro mirror high resolution, the former has various light such as difference, dark field, bright-field, polarisation, Midst density bright-field, continuous videos amplification
Function is learned, in addition to checking the routine microcirculation such as microcirculation form, fluidised form, ambient state, also in microcirculation cytorheology
Red blood cell, blood platelet, leucocyte, the rheological behaviour of blood plasma observed and quantitative analysis, but do not occurred also in the prior art
The device of the animal mesenterium Microcirculatory Observations supporting with it.
Chinese patent " rat mesenteria microcirculation observation constant-temperature perfusion device "（Patent No. ZL2005201441671）
There is provided a kind of constant temperature perfusion device used for observation experiment, but device observation site can not realize constant temperature and humidity, and should
Device is provided with the structures such as sliding door, and device overall volume is excessive, is difficult to be placed on microscope observation platform, it is impossible to microscope
Closely firm connection.
The content of the invention
The invention provides a kind of small volume, constant temperature and humidity, it is easy to keep mesenterium sample cleaning, reality can be fixedly secured
Test animal and with the close-fitting perfusion device of microscope, present invention simultaneously provides the application of the device.
The present invention solve its technical problem use technical scheme be：
Constant temperature and humidity perfusion device, including perfusion box, fixed plate and temperature control are used in a kind of Rat Mesenteric Microcirculation observation
Device processed；
The perfusion box includes observation area, heating zone and clear area, and Microcirculatory Observations window is set in the observation area, described
Microcirculatory Observations window sets temperature-sensing probe higher than plane where observation area and printing opacity in the observation area；Set in the heating zone
Electrothermal tube is put, the heating zone is connected with observation area, and connectivity slot is set between heating zone and observation area；
More than one screw and the bolt coordinated with the screw are set in the fixed plate, more than four are preferable to provide
And at least four be uniformly arranged on fixed plate corner, also set up holding the observation draw-in groove on edge and is used for outside window in Microcirculatory Observations
Block connection groove edge and positioned at the fixture block of fixed plate bottom surface；
The temperature control equipment is connected with electrothermal tube and temperature-sensing probe by wire.
Preferably, the perfusion box also includes being arranged on the fixed area in outside, the outer of the fixed area set with it is micro-
The fixing groove of mirror objective table sample slice clamper fixing threaded hole matching.
Preferably, the fixed plate sets the fixed card slot matched with microscope handel in the opposite edge of observation draw-in groove.
Preferably, the Microcirculatory Observations window is fixedly connected by connecting pole with the base plate of observation area, and the connecting pole is
Ground glass material.
Preferably, temperature controlled region is set on the perfusion box, and the wire of electrothermal tube and temperature-sensing probe is by temperature controlled region and temperature
Controller is connected, it is to avoid wire influences service life in atmosphere with the tie point exposure of electrothermal tube and temperature probe.
Present invention simultaneously provides the application of the perfusion device, it is used for microexamination animal Mesentery microcirculation.
Further, the Microcirculatory Observations for being carried out for the multinomial microscopic systems of Bu Laide.
Model TDA8002 type temperature control of the temperature control equipment of the present invention from the production of Kai Hua Intelligent Instruments Watch Factory
Instrument.
The observation window is the good glass or resin of translucency, for the light source through microscope peep hole.
The beneficial effects of the present invention are：
（1）Keep the temperature and humidity in perfusion device constant using electrothermal tube, temperature-sensing probe and temperature control equipment；
（2）Small volume, is placed conveniently within microscopical observation platform, it is easy to mobile, and operation is flexible；
（3）Observation draw-in groove in fixed plate realizes the mating connection with perfusion box by holding in the outer of connecting pole, even
Closely stabilization is connect, fixed plate covers above perfusion box the evaporation that can reduce solution in perfusion box；
（4）The fixture block of fixed plate coordinates with connectivity slot, and fixture block is stuck in two sides of connectivity slot, prevents fixed plate and perfusion
Box horizontally slips；
（5）Microcirculatory Observations window is transparent light-transmitting materials, and connecting pole is frosted material, and the light of microscope peep hole can
Effectively by Microcirculatory Observations window, connecting pole reduces loss of light source, improves the definition of observation；
（6）The groove with microscopes is respectively provided with perfusion box and fixed plate, with microscope stable connection；
（7）Clear area provides the storage space of cleaning fluid, convenient to draw clean solution from clear area in experimentation, clearly
Intestinal lavage mesenteric tissue, improves the definition of tissue.
Rat Mesenteric Microcirculation observation constant temperature and humidity perfusion device of the present invention, small volume, space layout utilizes reasonable,
Constant temperature and humidity effect can be effectively reached, micro- sem observation animal intestine mesentery microcirculation is particularly well-suited to, it is preceding with good application
Scape.
Brief description of the drawings
Fig. 1 is the structural representation of embodiment 1；
Fig. 2 is the structural representation of the perfusion box of embodiment 1；
Fig. 3 is the structural representation of embodiment 1；
Fig. 4 is the structural representation of the perfusion box of embodiment 2.
In accompanying drawing, 1 perfusion box, 2 fixed plates, 3 temperature control equipments, 4 observation areas, 5 heating zones, 6 clear areas, 7 microcirculations
Observation window, 8 temperature-sensing probes, 9 electrothermal tubes, 10 screws, 11 observation draw-in grooves, 12 fixed areas, 13 fixing grooves, 14 fixed card slots, 15 connect
Connect post, 16 bolts, 17 connectivity slots, 18 temperature controlled regions, 19 fixture blocks.
Specific embodiment
To enable the above objects, features and advantages of the present invention more obvious understandable, implement below in conjunction with the present invention
The drawings and specific embodiments in example, are clearly and completely described to the technical scheme in the embodiment of the present invention, it is clear that
Described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on the implementation in the present invention
Example, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made is belonged to
The scope of protection of the invention.
Embodiment 1
As Figure 1-3, embodiment 1 includes perfusion box 1, fixed plate 2 and temperature control equipment 3；
The perfusion box 1 includes observation area 4, heating zone 5, clear area 6, fixed area 12 and temperature controlled region 18, the fixed area
12 sides for being arranged on heating zone 5, clear area 6 and fixed area 12 are arranged on the opposite side of heating zone 5；
Temperature-sensing probe 8 and Microcirculatory Observations window 7 are set in the observation area 4, and the Microcirculatory Observations window 7 is higher than observation area
4 place planes and printing opacity, the Microcirculatory Observations window 7 are fixedly connected by connecting pole 15 with the base plate of observation area 4, the connection
Post 15 is ground glass material；Electrothermal tube 9 is set in the heating zone 5, and the heating zone 5 connects with observation area 4, heating zone 5
Connectivity slot 17 is set and observation area 4 between；The outer of the fixed area 12 is set to be consolidated with microscope carrier sample slice clamper
Determine the fixing groove 13 of screw matching.
More than one screw 10 and the bolt 16 coordinated with the screw 10, the fixed plate 2 are set in the fixed plate 2
Side is provided with observation draw-in groove 11 of the holding in the outer of Microcirculatory Observations window 7, and opposite side is set and consolidating that microscope handel is matched
Determine draw-in groove 14, the shape of the observation draw-in groove 11 is consistent with the outer shape of connecting pole 15, size with size, the fixed plate 2 and
The one side of the contact of perfusion box 1 is provided with fixture block 19, and the fixture block 19 sets two, and fixed plate 2 covers fixture block 19 when on perfusion box 1
Positioned at the both sides inner side of connectivity slot 17, prevent fixed plate 2 from being slided with respect to perfusion box 1；
The temperature control equipment 3 is connected with electrothermal tube 9 and the wire of temperature-sensing probe 8, and the temperature control equipment 3 is from triumphant
The model TDA8002 type temperature controllers of magnificent Intelligent Instrument Watch Factory production.
The Microcirculatory Observations window 7 is the good glass of translucency, for the light source through microscope peep hole.
The wire of electrothermal tube 9 and temperature-sensing probe 8 is connected by temperature controlled region 18 with temperature controller, it is to avoid wire and electrothermal tube
9 and temperature probe 8 tie point exposure influence service life in atmosphere.
Embodiment 1 is used for microexamination animal Mesentery microcirculation, is particularly well-suited to the multinomial microscopic systems of Bu Laide and enters
Capable Microcirculatory Observations.When using, perfusion box 1 is placed on microscopical objective table, the cutting ferrule of fixing groove 13 of fixed area 12
Outside microscope carrier sample slice clamper fixing threaded hole, the aligming microscope objective lens of Microcirculatory Observations window 7.To heating zone
5th, physiological saline is added in observation area 4 and clear area 6, opens temperature control equipment 3, electrothermal tube 9 is heated to physiological saline.
Experimental animal is fixed in fixed plate 2, animal is bound with fixing band, and by fixing band using in fixed plate 2
Screw 10 and bolt 16 fix.Fixed plate 2 is placed on the top of perfusion box 1, fixed card slot 14 is stuck on microscope handel, is seen
The holding of draw-in groove 11 is examined in the outer of connecting pole 15.
The mesenterium of experimental animal is placed on Microcirculatory Observations window 7, the normal saline flushing with clear area 6 is clean,
The physiological saline of heating zone 5 and observation area 4 provides the environment of constant temperature and humidity for mesentery tissue, keeps consistent inside and outside organizer
Property and stability.Microcirculatory Observations window 7 is the good light-transmitting materials of translucency, is easy to microscope light source to pass through, and improves observation
Definition.
Embodiment 2
As shown in figure 4, with reference to Fig. 1-3, embodiment 2 as different from Example 1, the fixed area 12 of the perfusion box 1 of embodiment 1
With heating zone 5 in the same side of observation area 4, the fixed area 12 of embodiment 2 and heating zone 5 are respectively in the opposite sides of observation area 4；
The fixed plate 2 of embodiment 1 is provided with fixed card slot 14, and embodiment 2 is not provided with fixed card slot 14.
Embodiment 2 be applied to experimental animal volume it is larger when, fixed plate 2 is difficult to be placed on the load of micro objective and handel
Between thing platform.When using, perfusion box 1 is placed on microscopical objective table, the fixing groove 13 of fixed area 12 is fastened in micro-
Mirror objective table sample slice clamper fixing threaded hole matches outer, the aligming microscope objective lens of Microcirculatory Observations window 7；Fixed plate 2
The top of heating zone 5 is placed on, the observation holding of draw-in groove 11 is in the outer of connecting pole 15.
Other structures are same as Example 1 with operating method.
The fixation of embodiment 2,2 are arranged on perfusion box 1 and on the outside of microscope, are that the fixed plate 2 of large area is provided
Sufficient space, can place the larger experimental animal of volume.
Claims (5)
1. constant temperature and humidity perfusion device is used in a kind of Rat Mesenteric Microcirculation observation, it is characterised in that including perfusion box（1）, it is solid
Fixed board（2）And temperature control equipment（3）；
The perfusion box（1）Including observation area（4）, heating zone（5）And clear area（6）, the observation area（4）Interior setting microcirculation
Observation window（7）, the Microcirculatory Observations window（7）Higher than observation area（4）Place plane and printing opacity, the observation area（4）Interior setting
Temperature-sensing probe（8）；The heating zone（5）Interior setting electrothermal tube（9）, the heating zone（5）With observation area（4）Connection, heating zone
（5）And observation area（4）Between connectivity slot is set（17）；
The fixed plate（2）Upper more than one screw of setting（10）And the bolt coordinated with the screw（16）, also set up holding
In Microcirculatory Observations window（7）The observation draw-in groove of outer（11）With for blocking connectivity slot（17）Edge, positioned at fixed plate（2）Under
The fixture block of bottom surface（19）；
The temperature control equipment（3）With electrothermal tube（9）And temperature-sensing probe（8）Wire is connected；
The perfusion box（1）Also include being arranged on the fixed area of its side（12）, the fixed area（12）Outer set and aobvious
The fixing groove of micro mirror objective table connection（13）.
2. perfusion device according to claim 1, it is characterised in that the fixed plate（2）In observation draw-in groove（11）Phase
The fixed card slot matched with microscope handel is set to one side（14）.
3. perfusion device according to claim 1, it is characterised in that the Microcirculatory Observations window（7）By connecting pole
（15）With observation area（4）Base plate be fixedly connected, the connecting pole（15）It is ground glass material.
4. the application of the perfusion device described in any one of claim 1-3, it is characterised in that for microexamination animal mesenterium
Microcirculation.
5. application according to claim 4, it is characterised in that for the microcirculation that the multinomial microscopic systems of Bu Laide are carried out
Observation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510436733.4A CN104921838B (en) | 2015-07-23 | 2015-07-23 | A kind of Mesentery microcirculation observation constant temperature and humidity perfusion device and its application |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510436733.4A CN104921838B (en) | 2015-07-23 | 2015-07-23 | A kind of Mesentery microcirculation observation constant temperature and humidity perfusion device and its application |
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| CN104921838B true CN104921838B (en) | 2017-06-06 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105676461A (en) * | 2016-04-05 | 2016-06-15 | 皖南医学院 | Animal mesentery microcirculation observation system |
| CN110037881A (en) * | 2019-05-16 | 2019-07-23 | 上海长征医院 | A kind of stage apparatus for the observation of mouse cremaster |
| CN113907907A (en) * | 2021-09-30 | 2022-01-11 | 广东省科学院健康医学研究所 | Mouse dorsal spine skin pleat window model system and microcirculation constant temperature control method |
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| CN2922834Y (en) * | 2005-12-22 | 2007-07-18 | 覃建民 | Rat mesenteria microcirculation observation constant-temperature perfusion device |
| CN203841834U (en) * | 2014-04-15 | 2014-09-24 | 上海市同济医院 | Multifunctional constant-temperature operation perfusion device |
| CN104146789A (en) * | 2014-07-17 | 2014-11-19 | 梁建民 | Constant-temperature heating plate and experiment table |
| CN203953871U (en) * | 2014-07-31 | 2014-11-26 | 王晓萃 | Experiment is poured into fixture with rat |
| CN204839817U (en) * | 2015-07-23 | 2015-12-09 | 河北医科大学第一医院 | Mesenterium microcirculation is observed with constant temperature and humidity perfusion device |
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