CN104893966A - Automatic plasmid extraction device with compact structure - Google Patents
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- 239000013612 plasmid Substances 0.000 title claims abstract description 37
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- 210000004907 gland Anatomy 0.000 claims abstract description 23
- 238000010276 construction Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 description 32
- 239000000243 solution Substances 0.000 description 19
- 239000002699 waste material Substances 0.000 description 16
- 238000001179 sorption measurement Methods 0.000 description 12
- 238000000034 method Methods 0.000 description 11
- 230000001580 bacterial effect Effects 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 239000013611 chromosomal DNA Substances 0.000 description 2
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- 239000003480 eluent Substances 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
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- 238000007906 compression Methods 0.000 description 1
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- 238000010828 elution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000012215 gene cloning Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
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Abstract
Description
本发明是申请号为201310637644.7、申请日为2013年11月29日、发明创造名称为“自动质粒提取装置”的发明专利申请的分案申请。 The present invention is a divisional application of the invention patent application with the application number 201310637644.7, the application date is November 29, 2013, and the invention name is "automatic plasmid extraction device".
技术领域 technical field
本发明涉及生物实验设备领域，特别是一种自动质粒提取装置。 The present invention relates to the field of biological experiment equipment, especially an automatic plasmid extraction device.
背景技术 Background technique
质粒是附加到细胞中的非细胞的染色体或核区DNA原有的能够自主复制的较小的DNA分子, 它是把一个有用的目的DNA片段通过重组DNA技术，送进受体细胞中去进行繁殖和表达的常用载体。因而质粒提取是基因克隆过程中的一个常规操作，目前通常使用碱裂解法从大肠杆菌制备质粒。原理为：使用高pH值溶液使质粒DNA和染色体DNA变性，同时沉淀蛋白质。再将pH值调至中性，质粒DNA较小，很容易复性成双链。而染色体DNA较大，不会复性，缠结成网状不溶物质，从而可以通过离心除去。一般步骤为将细菌培养液离心获得菌体，加入重悬液，再加入新配制的细菌裂解溶液，最后加入用冰预冷的用于中和PH的中和溶液，然后离心，去除沉淀，在上清液中加入DNA吸附柱，离心去除废液，再加入洗液1洗一遍，离心去除废液，洗液2洗2遍，离心去除废液，最后用洗脱液洗脱得到纯的质粒DNA。从中可见操作步骤繁琐，每提取20个质粒需要开盖关盖多达400多次，离心10余次。由于质粒提取是从事分子生物学研究的实验室每天都要用的常规技术，因而工作量较大，又由于其机械重复性较高，为自动化提供了可能。 A plasmid is a small DNA molecule that is attached to the non-cellular chromosome or nuclear region DNA of the cell and can replicate autonomously. It is a useful target DNA fragment that is sent into the recipient cell through recombinant DNA technology. Common vector for propagation and expression. Therefore, plasmid extraction is a routine operation in the process of gene cloning. Alkaline lysis is usually used to prepare plasmids from Escherichia coli. The principle is: using a high pH solution to denature plasmid DNA and chromosomal DNA while precipitating proteins. Then adjust the pH value to neutral, the plasmid DNA is small, and it is easy to anneal into double strands. Chromosomal DNA is relatively large, will not refold, and will tangle into a network of insoluble matter, which can be removed by centrifugation. The general procedure is to centrifuge the bacterial culture solution to obtain the bacteria, add the resuspension solution, then add the newly prepared bacterial lysis solution, and finally add the neutralization solution pre-cooled with ice for neutralizing the pH, then centrifuge to remove the precipitate, and then Add DNA adsorption column to the supernatant, centrifuge to remove waste liquid, then add washing solution 1 to wash once, centrifuge to remove waste liquid, wash with washing liquid 2 twice, centrifuge to remove waste liquid, and finally elute with eluent to obtain pure plasmid DNA. It can be seen that the operation steps are cumbersome, and every extraction of 20 plasmids requires opening and closing the cover more than 400 times, and centrifugation more than 10 times. Because plasmid extraction is a routine technique used every day in laboratories engaged in molecular biology research, the workload is relatively large, and because of its high mechanical repeatability, it provides the possibility for automation.
发明内容 Contents of the invention
本发明的目的在于针对背景技术中所述的现有的质粒提取过程人工操作重复性高，工作量大的问题，提供一种能够解决前述问题的结构紧凑的自动质粒提取装置。 The object of the present invention is to provide a compact automatic plasmid extraction device capable of solving the aforementioned problems in view of the problems of high manual operation repeatability and heavy workload in the existing plasmid extraction process described in the background technology.
能够实现本发明的发明目的的技术方案如下： The technical scheme that can realize the purpose of the invention of the present invention is as follows:
一种结构紧凑的自动质粒提取装置，其包括主控制器、离心振动装置、以及分别设置于离心振动装置两侧的加液取液装置和升降压盖旋转升降机构，离心振动装置上设置有离心座，离心座上设置有多个离心管槽，其中一个离心管槽旁设置定位感应器，离心座上设置有与定位感应器对应的传感器，升降压盖旋转升降机构包括可旋转的支架和可升降的升降压盖，支架为L形，升降压盖连接在支架上，升降压盖下面设置有与离心管槽对应的离心管塞，加液取液装置包括可转动的L形支架和设置于L形支架上的移液盘，移液盘上设置有多个加液管和吸液管，加液管上连接电磁阀； An automatic plasmid extracting device with a compact structure, which includes a main controller, a centrifugal vibration device, a liquid feeding and taking device and a lifting gland rotating and lifting mechanism respectively arranged on both sides of the centrifugal vibration device, and the centrifugal vibration device is provided with Centrifugal seat, a plurality of centrifuge tube slots are arranged on the centrifugal seat, a positioning sensor is set beside one of the centrifuge tube slots, a sensor corresponding to the positioning sensor is set on the centrifugal seat, and the lifting gland rotating lifting mechanism includes a rotatable bracket And the lifting gland that can be lifted, the bracket is L-shaped, the lifting gland is connected to the bracket, the centrifuge tube plug corresponding to the centrifuge tube groove is arranged under the lifting gland, and the liquid adding and taking device includes a rotatable L Shaped bracket and a pipetting tray arranged on the L-shaped bracket, the pipetting tray is provided with a plurality of liquid feeding pipes and suction pipes, and the liquid feeding pipe is connected to a solenoid valve;
上述的离心振动装置包括底座、振动电机、振动座以及离心机； The above-mentioned centrifugal vibrating device includes a base, a vibrating motor, a vibrating seat and a centrifuge;
上述的离心座上设置离心管槽为偶数个；升降压盖下方对应设置有相同数量的离心管塞；根据具体提取ＤＮＡ质粒的过程的不同，需要设置不同数量的离心管槽； There are an even number of centrifuge tube slots on the above centrifugal seat; the same number of centrifuge tube plugs are correspondingly set under the lifting gland; different numbers of centrifuge tube slots need to be set according to the specific process of extracting DNA plasmids;
上述的加液取液装置上设置有6个加液管和两个吸液管；根据具体提取ＤＮＡ质粒的过程的不同，需要设置不同数量的加液管和吸液管。 The above liquid feeding and taking device is provided with 6 liquid feeding pipes and two pipetting pipes; according to the specific process of extracting DNA plasmids, different numbers of pipetting pipes and pipetting pipes need to be provided.
为了便于对废液进行集中处理，上述的离心座上位于加液取液装置附近设置有废液槽。 In order to facilitate centralized treatment of the waste liquid, a waste liquid tank is provided on the above-mentioned centrifugal seat near the liquid adding and taking device.
本发明的结构紧凑的自动质粒提取装置的工作原理为：通过主控器来控制离心振动装置的旋转和振动、加液取液装置的旋转和升降以及对离心管内注入和取出液体、升降压盖旋转升降机构的旋转和升降，电磁阀的开关，离心管塞的压紧和松开以及离心盖的开关。离心座上根据需要可设置有偶数个离心管槽，使用时，将离心管和DNA吸附柱间隔放入离心管槽内，下面以离心座上设置四个离心管槽为例进行说明，四个离心管槽顺次编号为一号至四号；一号离心管槽旁有定位感应器，与离心工作台上相应的传感器感应后能自动停在一号的位置上。在提取质粒时，首先，人工将洁净的一号离心管和三号离心管分别放入一号离心管槽和三号离心管槽内，将二号DNA吸附柱和四号DNA吸附柱分别放入二号离心管槽和四三号离心管槽内，并将已经发酵好的同等体积的细菌培养液（约1.5mL）加入一号和三号离心管内，然后转动升降压盖旋转升降机构的支架，将升降压盖从离心座的侧面水平转动到离心座的上方，再下移升降压盖，将预先安置在升降压盖下面的四个离心管塞松开后压紧到四个对应的离心管上，然后支架上移并将升降压盖水平转动至原来的位置。然后，关紧离心盖，离心振动装置对一号和三号离心管内的细菌培养液旋转离心；离心完成后，离心盖开启，升降压盖从离心座的侧面水平转动到离心座的上面，再下移将四个离心管塞同时松开并固定在升降压盖下面，升降压盖上移，再水平转动支架至原来的位置，这时细菌沉淀在一号和三号离心管的底部，随后加液取液装置的Ｌ形支架转动，将移液盘水平转动到离心座的上面,再转动移液盘使第一废液吸管转动至一号离心管正上方，下移移液盘，将第一废液吸管末端预先装上的一次性用的长塑料管慢慢进入一号离心管，将其中的上清液体慢慢吸干，然后转动移液盘到废液槽上方，将废液排到废液槽内，再转动移液盘使第一废液吸管转动至三号离心管正上方，下移移液盘将第一废液吸管下端的长塑料管慢慢进入三号离心管，将其中的上清液体也慢慢吸干并排到废液槽中。然后移液盘旋转使第一加液管移动到一号离心管正上方并通过电磁阀控制加入定量的重悬液250μl，然后加同等体积的重悬液到三号离心管中，按上述的方式，将离心管塞压在离心管上，启动离心振动装置，离心管剧烈振荡1分钟，使细菌菌体均匀悬浮，再旋转移液盘使第二加液管分别移动到一号离心管和三号离心管上方并通过电磁阀控制加入新配制的细菌裂解溶液250μl，通过离心振动装置按照上述的方法轻轻振荡离心管使溶液混匀，最后旋转移液盘使第三加液管分别移动到一号离心管和三号离心管上方并通过电磁阀控制加入用冰预冷的用于中和pH的中和溶液350μl，通过离心振动装置按照上述的方法轻轻振荡离心管使溶液混匀后离心。离心结束后，移动和旋转移液盘使第二吸液管末端预先装上的一次性用的长塑料管伸至一号离心管中将其中含有质粒DNA的上清液体慢慢吸出，再转动移液盘将第二吸液管中的含有质粒DNA的上清液体慢慢加到二号DNA吸附柱中，同样用第二吸液管将三号离心管中的液体吸出并转移到四号DNA吸附柱中，然后离心，这时质粒DNA会吸附到DNA吸附柱中DNA结合膜上，而滤过的废液收集在DNA吸附柱的下部。然后转动移液盘使第四加液管依次移动到二号DNA吸附柱和四号DNA吸附柱的上方并通过控制电磁阀向DNA吸附柱中加入含有异丙醇的第一洗液清洗一遍去除杂蛋白，然后离心，再转动移液盘从第五加液管向DNA吸附柱中再加入含有乙醇的第二洗液，洗两遍以上去除杂蛋白，然后离心，人工取出DNA吸附柱上部，将收集有废液的DNA吸附柱下部取出丢弃，换放一个新的DNA收集管，将含有质粒DNA的DNA吸附柱上部放入DNA收集管中，从第六加液管分别加入洗脱液到二号和四号DNA收集管中，然后离心洗脱后，在DNA收集管中得到纯的质粒DNA，人工取出含有质粒DNA的收集管保存备用。这样一次操作可以提取两倍体积的质粒DNA，增加了产量，节约了时间。上述以四个离心管为例，但根据实际需要，离心座中可以设置更多的离心管槽，以增加更高的产量，也可以根据需要，同时提取几种不同的质粒DNA。 The working principle of the compact automatic plasmid extracting device of the present invention is as follows: the rotation and vibration of the centrifugal vibrating device, the rotation and lifting of the liquid feeding and liquid taking device, and the injection and removal of liquid in the centrifuge tube, and the lifting and lowering of the pressure are controlled by the main controller. The rotation and lifting of the cover rotation and lifting mechanism, the switch of the solenoid valve, the compression and release of the centrifuge tube plug and the switch of the centrifuge cover. There can be an even number of centrifuge tube slots on the centrifuge base as needed. When in use, put the centrifuge tubes and DNA adsorption columns into the centrifuge tube slots at intervals. The following is an example of setting four centrifuge tube slots on the centrifuge base. Four The centrifuge tube slots are numbered from No. 1 to No. 4 in sequence; there is a positioning sensor next to the No. 1 centrifuge tube slot, which can automatically stop at the No. 1 position after being sensed by the corresponding sensor on the centrifuge workbench. When extracting the plasmid, first, manually put the clean No. 1 and No. 3 centrifuge tubes into the No. 1 and No. 3 centrifuge tube slots respectively, and put the No. Put it into the No. 2 centrifuge tube tank and the No. 4 and No. 3 centrifuge tube tank, and add the same volume of fermented bacterial culture solution (about 1.5mL) into the No. 1 and No. 3 centrifuge tubes, and then turn the lifting gland to rotate the lifting mechanism the bracket, turn the lifting gland horizontally from the side of the centrifugal seat to the top of the centrifugal seat, then move the lifting gland down, loosen the four centrifuge tube plugs that are pre-installed under the lifting gland and press them to the Four corresponding centrifuge tubes, then the bracket moves up and the lifting gland is turned horizontally to the original position. Then, close the centrifuge cover tightly, and the centrifugal vibration device rotates and centrifuges the bacterial culture solution in the No. 1 and No. 3 centrifuge tubes; Move down to loosen the four centrifuge tube plugs at the same time and fix them under the lifting gland, move the lifting gland up, and then turn the bracket horizontally to the original position. At this time, the bacteria settle at the bottom of the No. 1 and No. 3 centrifuge tubes , and then the L-shaped bracket of the liquid addition and removal device rotates, and the pipetting tray is rotated horizontally to the top of the centrifugal seat, and then the pipetting tray is rotated so that the first waste liquid suction pipe is rotated to the top of the No. 1 centrifuge tube, and the pipetting tray is moved down. , slowly put the disposable long plastic tube pre-installed on the end of the first waste liquid suction pipe into the No. Drain the waste liquid into the waste liquid tank, then turn the pipetting plate to make the first waste liquid suction pipe rotate to the top of the No. Centrifuge tubes, blot the supernatant liquid slowly and drain into the waste tank. Then the pipette rotates to move the first pipette to the top of the No. 1 centrifuge tube and add a quantitative resuspension solution of 250 μl through the solenoid valve control, and then add the same volume of the resuspension solution to the No. 3 centrifuge tube. method, press the centrifuge tube stopper on the centrifuge tube, start the centrifugal vibration device, and vibrate the centrifuge tube vigorously for 1 minute to suspend the bacterial cells evenly, then rotate the pipette to move the second liquid feeding tube to the No. 1 centrifuge tube and Add 250 μl of newly prepared bacterial lysing solution to the top of the No. 3 centrifuge tube and control it through the solenoid valve, use the centrifugal vibration device to gently shake the centrifuge tube according to the above method to mix the solution, and finally rotate the pipette to move the third liquid addition tube separately Add 350 μl of ice-cooled neutralization solution to the top of the No. 1 and No. 3 centrifuge tubes and control the pH through the solenoid valve, and use the centrifugal vibration device to gently shake the centrifuge tubes according to the above method to mix the solution After centrifugation. After centrifugation, move and rotate the pipetting plate so that the disposable long plastic tube pre-installed at the end of the second pipette extends into the No. 1 centrifuge tube to slowly suck out the supernatant liquid containing plasmid DNA, and then rotate Add the supernatant liquid containing plasmid DNA in the second pipette to the No. 2 DNA adsorption column slowly, and use the second pipette to suck out the liquid in the No. At this time, the plasmid DNA will be adsorbed to the DNA binding membrane in the DNA adsorption column, and the filtered waste liquid will be collected at the lower part of the DNA adsorption column. Then turn the pipette to move the fourth pipette to the top of No. 2 DNA adsorption column and No. 4 DNA adsorption column in turn, and add the first washing solution containing isopropanol to the DNA adsorption column by controlling the solenoid valve to wash and remove it once. Miscellaneous proteins, then centrifuged, and then rotate the pipette to add the second washing solution containing ethanol from the fifth liquid addition tube to the DNA adsorption column, wash more than two times to remove the miscellaneous proteins, then centrifuge, manually remove the upper part of the DNA adsorption column, Take out the lower part of the DNA adsorption column that has collected waste liquid and discard it, replace it with a new DNA collection tube, put the upper part of the DNA adsorption column containing plasmid DNA into the DNA collection tube, and add the eluent from the sixth liquid addition tube to the No. 2 and No. 4 DNA collection tubes, and then after centrifugation and elution, pure plasmid DNA was obtained in the DNA collection tubes, and the collection tubes containing plasmid DNA were manually taken out and stored for later use. Such a single operation can extract twice the volume of plasmid DNA, increasing yield and saving time. Four centrifuge tubes are used as an example above, but according to actual needs, more centrifuge tube slots can be set in the centrifuge base to increase higher yields, and several different plasmid DNAs can also be extracted at the same time according to needs.
本发明的有益效果：本发明结构紧凑，整个提取质粒的过程主要通过机器来自动完成，不需要人工参与，质粒提取过程中，可以通过修改控制器的参数来调节各溶液及洗液的加入量，还可以调节离心时间和振动强度，可以调节清洗液的清洗次数，以提高提取的质粒的质量。 Beneficial effects of the present invention: the present invention has a compact structure, and the entire process of extracting plasmids is mainly automatically completed by machines without manual participation. During the process of plasmid extraction, the addition of each solution and lotion can be adjusted by modifying the parameters of the controller , you can also adjust the centrifugation time and vibration intensity, and you can adjust the washing times of the cleaning solution to improve the quality of the extracted plasmid.
附图说明 Description of drawings
图1为本发明的结构示意图； Fig. 1 is a structural representation of the present invention;
图2为发明中的离心振动装置的结构示意图。 Fig. 2 is a structural schematic diagram of the centrifugal vibration device in the invention.
上述附图中的附图标记如下：1为主控制器，2为离心座，３为离心管槽，４为定位感应器，5为传感器，6为升降压盖，7为离心管塞，8为L形支架，9为移液盘，10为加液管，11为吸液管，12为电磁阀，13为废液槽，14为底座，15为振动电机，16为振动座，17为离心机。 The reference signs in the above drawings are as follows: 1 is the main controller, 2 is the centrifugal seat, 3 is the centrifuge tube groove, 4 is the positioning sensor, 5 is the sensor, 6 is the lifting gland, 7 is the centrifuge tube plug, 8 is an L-shaped bracket, 9 is a pipetting tray, 10 is a liquid feeding pipe, 11 is a liquid suction pipe, 12 is a solenoid valve, 13 is a waste liquid tank, 14 is a base, 15 is a vibration motor, 16 is a vibration seat, 17 for the centrifuge.
具体实施方式 Detailed ways
下面结合附图1、附图2对本发明的具体实施方式进行进一步阐述： Below in conjunction with accompanying drawing 1, accompanying drawing 2 the specific embodiment of the present invention is further elaborated:
（实施例1） (Example 1)
如图1所示的一种结构紧凑的自动质粒提取装置，其包括主控制器1、离心振动装置、以及分别设置于离心振动装置两侧的加液取液装置和升降压盖旋转升降机构，离心振动装置上设置有离心座2，离心座2上设置有多个离心管槽3，其中一个离心管槽3旁设置定位感应器4，离心座2上设置有与定位感应器4对应的传感器5，所述的升降压盖旋转升降机构包括可旋转的支架和可升降的升降压盖6，支架为L形，升降压盖6连接在支架上，升降压盖6下面设置有与离心管槽3对应的离心管塞7，加液取液装置包括可转动的L形支架8和设置于L形支架8上的移液盘9，移液盘9上设置有多个加液管10和吸液管11，加液管10上连接电磁阀12。 A compact automatic plasmid extraction device as shown in Figure 1, which includes a main controller 1, a centrifugal vibration device, and a liquid feeding and taking device and a lifting gland rotating and lifting mechanism respectively arranged on both sides of the centrifugal vibration device , the centrifugal vibration device is provided with a centrifugal seat 2, and a plurality of centrifugal tube grooves 3 are arranged on the centrifugal seat 2, and a positioning sensor 4 is arranged beside one of the centrifugal tube grooves 3, and a positioning sensor 4 corresponding to the positioning sensor 4 is arranged on the centrifugal seat 2. Sensor 5, the lifting and lowering cover rotation and lifting mechanism includes a rotatable bracket and a liftable lifting and lowering cover 6, the bracket is L-shaped, the lifting and lowering cover 6 is connected to the bracket, and the lifting and lowering cover 6 is arranged below There is a centrifuge tube plug 7 corresponding to the centrifuge tube groove 3. The liquid adding and taking liquid device includes a rotatable L-shaped support 8 and a pipetting tray 9 arranged on the L-shaped support 8. The pipetting tray 9 is provided with a plurality of adding The liquid pipe 10 and the liquid suction pipe 11 are connected with a solenoid valve 12 on the liquid feeding pipe 10 .
（实施例2） (Example 2)
如图2所示，前述实施例1记载的方案中，离心振动装置包括底座14、振动电机15、振动座16以及离心机17。通过振动电机15带动振动座16振动，离心机17设置在振动座16上，通过这种设置可以实现振动和离心的操作，简化自动质粒提取装置的结构，使整个装置结构更加紧凑。 As shown in FIG. 2 , in the solution described in the aforementioned embodiment 1, the centrifugal vibration device includes a base 14 , a vibration motor 15 , a vibration base 16 and a centrifuge 17 . The vibrating base 16 is driven to vibrate by the vibrating motor 15, and the centrifuge 17 is arranged on the vibrating base 16. Vibration and centrifugal operations can be realized through this arrangement, which simplifies the structure of the automatic plasmid extraction device and makes the whole device more compact.
（实施例3） (Example 3)
前述实施例1记载的方案中，加液取液装置上设置有6个加液管10和两个吸液管11，离心座2上共设置4个离心管槽3，升降压盖6下方对应设置4个离心管塞7。 In the scheme described in the aforementioned embodiment 1, the liquid feeding and taking device is provided with 6 liquid feeding pipes 10 and two liquid suction pipes 11, and a total of 4 centrifuge tube grooves 3 are arranged on the centrifugal seat 2, and the lifting pressure cover 6 is below the Correspondingly, four centrifuge tube plugs 7 are provided.
（实施例4） (Example 4)
前述实施例1记载的方案中，为了便于对废液进行集中处理，所述的离心座2上位于加液取液装置附近设置有废液槽13。 In the scheme described in the aforementioned embodiment 1, in order to facilitate centralized treatment of the waste liquid, a waste liquid tank 13 is provided on the centrifuge base 2 near the liquid adding and taking device.
Claims (1)
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| CN201310637644.7A CN103602582B (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid-extracting device |
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| CN201310637644.7A Division CN103602582B (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid-extracting device |
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| CN104893966A true CN104893966A (en) | 2015-09-09 |
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| CN201510339214.6A Pending CN104893969A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extracting device with compact structure |
| CN201510339212.7A Pending CN104893968A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extraction device with high extraction quality |
| CN201510338181.3A Pending CN105018340A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extracting apparatus with high extracting quality |
| CN201510339040.3A Active CN104893967B (en) | 2013-11-29 | 2013-11-29 | Labor-saving automatic plasmid extraction element |
| CN201310637644.7A Expired - Fee Related CN103602582B (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid-extracting device |
| CN201510339213.1A Pending CN104928166A (en) | 2013-11-29 | 2013-11-29 | Automatic parameter-adjustable plasmid extracting device |
| CN201510338858.3A Pending CN104893966A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extraction device with compact structure |
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| CN201510338181.3A Pending CN105018340A (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid extracting apparatus with high extracting quality |
| CN201510339040.3A Active CN104893967B (en) | 2013-11-29 | 2013-11-29 | Labor-saving automatic plasmid extraction element |
| CN201310637644.7A Expired - Fee Related CN103602582B (en) | 2013-11-29 | 2013-11-29 | Automatic plasmid-extracting device |
| CN201510339213.1A Pending CN104928166A (en) | 2013-11-29 | 2013-11-29 | Automatic parameter-adjustable plasmid extracting device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106925439A (en) * | 2017-05-08 | 2017-07-07 | 浙江兰博生物科技股份有限公司 | A kind of flat hanging bag centrifuge |
| CN107115977A (en) * | 2017-05-08 | 2017-09-01 | 浙江兰博生物科技股份有限公司 | A kind of centrifuge |
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- 2013-11-29 CN CN201510338181.3A patent/CN105018340A/en active Pending
- 2013-11-29 CN CN201510339040.3A patent/CN104893967B/en active Active
- 2013-11-29 CN CN201310637644.7A patent/CN103602582B/en not_active Expired - Fee Related
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Also Published As
| Publication number | Publication date |
|---|---|
| CN104893967B (en) | 2019-07-23 |
| CN103602582B (en) | 2015-07-15 |
| CN104893967A (en) | 2015-09-09 |
| CN104893969A (en) | 2015-09-09 |
| CN104893968A (en) | 2015-09-09 |
| CN103602582A (en) | 2014-02-26 |
| CN105018340A (en) | 2015-11-04 |
| CN104928166A (en) | 2015-09-23 |
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