CN104824591A - Preparation method for probiotics-containing low-salt and flower-free pickled vegetables - Google Patents
Preparation method for probiotics-containing low-salt and flower-free pickled vegetables Download PDFInfo
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Abstract
本发明公开了一种含益生菌的低盐不生花泡菜的制备方法,包括以下步骤:A将待发酵蔬菜清洗沥干、切成小块;B待发酵蔬菜入坛、压实;C加入香辛料及泡菜液,使其没过待发酵蔬菜;泡菜液的配制:1%-5%食盐、0.05%-0.15%泡菜专用菌粉剂、1%-8%糖、1%-8%白酒、0.1%-0.3%食品级氯化钙、余量为水;D密封发酵坛,水封坛盖,在6℃-40℃厌氧发酵24h-240h。本发明利用人工混种发酵技术制备泡菜,发酵风味浓郁,生产工艺简单,大大缩短发酵周期,产品中食盐和亚硝酸盐含量明显降低,活益生菌数量较高,不产生白色菌斑,提高了泡菜生产的安全性,适合工业化生产。The invention discloses a preparation method of low-salt non-flowering kimchi containing probiotics, which comprises the following steps: A. washing, draining and cutting the vegetables to be fermented into small pieces; B. putting the vegetables to be fermented into an altar and compacting them; C. adding spices and pickle liquid, so that it is not over the vegetables to be fermented; the preparation of pickle liquid: 1%-5% salt, 0.05%-0.15% pickle special bacteria powder, 1%-8% sugar, 1%-8% liquor, 0.1% -0.3% food-grade calcium chloride, the balance is water; D seal the fermentation altar, seal the altar lid with water, and ferment anaerobically at 6°C-40°C for 24h-240h. The invention utilizes artificial mixed-breed fermentation technology to prepare kimchi, which has strong fermentation flavor, simple production process, greatly shortens the fermentation cycle, significantly reduces the content of salt and nitrite in the product, has a high number of live probiotics, does not produce white plaques, and improves the production efficiency of pickles. The safety of kimchi production is suitable for industrialized production.
Description
技术领域technical field
本发明涉及一种不生花泡菜的制备方法,具体涉及一种含益生菌的低盐不生花泡菜的制备方法,属于生物及食品加工技术领域。The invention relates to a method for preparing flowerless pickles, in particular to a method for preparing low-salt flowerless pickles containing probiotics, and belongs to the technical field of biology and food processing.
背景技术Background technique
泡菜是中国传统发酵食品的典型代表,具有悠久的历史,作为中国传统食品的瑰宝,泡菜是以新鲜蔬菜为原料,添加或不添加辅料,经食盐或食盐水泡渍发酵,配以泡渍液或调配液等加工而成。Kimchi is a typical representative of traditional Chinese fermented food with a long history. As a treasure of traditional Chinese food, pickled vegetables are made of fresh vegetables, with or without auxiliary materials, soaked and fermented with salt or brine, and served with pickling liquid or It is processed by mixing liquid and so on.
传统工艺制作的泡菜采用自然发酵,其发酵周期长,发酵条件不易控制,易染杂菌腐烂、变味,食用安全性差,不适合现代化连续式工业生产。且传统自然发酵泡菜由于酵母菌大量繁殖,极易在泡菜液表面出现白色团状菌斑,造成“生花”现象,导致泡菜变质甚至不能食用。现在生产的泡菜很多都是经过热杀菌再加防腐剂来延长货架期,但是,泡菜经过热杀菌后,大量乳酸菌已经被杀死,蛋白质、酶等营养物质已变性,产品的脆性下降,风味变差,而且,化学防腐剂本身对人体也有副作用。The kimchi produced by traditional crafts adopts natural fermentation, which has a long fermentation period, difficult control of fermentation conditions, easy to be contaminated with bacteria, rot, taste, poor food safety, and is not suitable for modern continuous industrial production. And traditional natural fermented kimchi is very easy to appear white cluster bacterial spot on the surface of kimchi liquid due to the large number of yeasts, causing "flowering" phenomenon, causing kimchi to deteriorate or even inedible. Many kimchi produced now are heat sterilized and preservatives are added to prolong the shelf life. However, after the kimchi is heat sterilized, a large number of lactic acid bacteria have been killed, proteins, enzymes and other nutrients have been denatured, and the brittleness of the product has decreased and the flavor has changed. Poor, and chemical preservatives themselves have side effects on the human body.
目前,我国传统泡菜原料预处理一般采用高盐盐渍,虽然可以抑制腐败菌繁殖、降低亚硝酸盐含量,但同时抑制了乳酸菌的发酵作用,蔬菜中的总酸较低,产品风味单一,也不利于盐渍原料长时间贮存。由于含盐量很高,需要增加后续的脱盐工序以降低蔬菜中的含盐量。At present, the pretreatment of traditional kimchi raw materials in my country generally adopts high-salt salting. Although it can inhibit the reproduction of spoilage bacteria and reduce the content of nitrite, it also inhibits the fermentation of lactic acid bacteria. The total acid in vegetables is low, and the product flavor is single. It is not conducive to long-term storage of salted raw materials. Due to the high salt content, it is necessary to increase the subsequent desalination process to reduce the salt content in vegetables.
蔬菜在传统腌制发酵过程中,以乳酸发酵为主,同时存在少量酒精发酵和醋酸发酵。适度发酵可以使蔬菜中的硝酸盐还原为亚硝酸盐,具有一定防腐功能,又能带来一定的酸味和香味,但过度发酵产生的大量亚硝酸盐在人体内将转变为致癌物质。腌制过程中若酒精发酵和醋酸发酵过度,还会消耗大量糖类物质,给腌制品风味和营养带来损失。In the traditional pickling and fermentation process of vegetables, lactic acid fermentation is the main method, and there is a small amount of alcoholic fermentation and acetic acid fermentation at the same time. Moderate fermentation can reduce the nitrate in vegetables to nitrite, which has a certain antiseptic function and can bring a certain sour taste and fragrance, but a large amount of nitrite produced by excessive fermentation will turn into carcinogens in the human body. If the alcohol fermentation and acetic acid fermentation are excessive during the pickling process, a large amount of sugars will be consumed, which will bring loss to the flavor and nutrition of the pickled products.
发明内容Contents of the invention
为解决上述技术问题,本发明提出了一种含益生菌的低盐不生花泡菜的制备方法,其生产成本较低、操作简单、发酵周期短、品质稳定、低盐、低亚硝酸盐、风味好且不易腐败变质。In order to solve the above-mentioned technical problems, the present invention proposes a method for preparing low-salt flower-free kimchi containing probiotics, which has low production cost, simple operation, short fermentation cycle, stable quality, low salt, low nitrite, and low flavor. Good and not easy to spoil.
为达到上述目的,本发明的技术方案如下:一种含益生菌的低盐不生花泡菜的制备方法,其包括以下加工步骤:In order to achieve the above object, the technical scheme of the present invention is as follows: a preparation method of low-salt flower-free kimchi containing probiotics, which comprises the following processing steps:
A、挑选新鲜、质嫩、无病虫害的新鲜蔬菜,洗净、沥干、切分,切成4cm长的小段;A. Select fresh, tender, and pest-free fresh vegetables, wash, drain, slice, and cut into 4cm-long pieces;
B、将切分好的蔬菜装入发酵坛中,压实;B. Put the cut vegetables into the fermentation jar and compact them;
C、向发酵坛中加入香辛料及蔬菜重量2-3倍的泡菜液,使其没过待发酵的蔬菜;C, add spices and the pickle liquid of 2-3 times of vegetables weight in the fermenting altar, make it submerge the vegetables to be fermented;
D、装坛完成后,密封发酵坛,用食盐水或者水封坛盖,保证发酵坛是内厌氧环境;D. After the altar is installed, seal the fermentation altar and seal the altar cover with salt water or water to ensure that the fermentation altar is in an anaerobic environment;
E、将发酵坛置于自动控温的恒温发酵室内,在6℃-40℃下厌氧发酵24h-240h。E. Place the fermentation jar in a constant temperature fermentation room with automatic temperature control, and perform anaerobic fermentation at 6°C-40°C for 24h-240h.
本发明采用人工接种发酵,可以控制恒定的发酵温度,大大缩短发酵周期,其发酵时间缩短为5-7天。The invention adopts artificial inoculation fermentation, can control the constant fermentation temperature, greatly shortens the fermentation cycle, and shortens the fermentation time to 5-7 days.
采用人工接种发酵,在发酵开始后,益生菌迅速成为优势菌群,不容易产生杂菌污染,产品中亚硝酸盐含量相对传统的自然发酵要低得多,大大降低了产品的安全隐患。Using artificial inoculation and fermentation, probiotics quickly become the dominant flora after the fermentation starts, and it is not easy to produce bacteria pollution. The nitrite content in the product is much lower than that of traditional natural fermentation, which greatly reduces the safety hazard of the product.
作为优选方案,在步骤C中,香辛料选自重量百分比为:1%-5%生姜、1%-5%大蒜、0.1%-2%八角、0.1%-2%桂皮和0.1%-1%花椒中的一种或两种以上,且上述均是按洗净沥干水分的新鲜蔬菜重量计算的。As a preferred version, in step C, the spices are selected from the following percentages by weight: 1%-5% ginger, 1%-5% garlic, 0.1%-2% star anise, 0.1%-2% cinnamon and 0.1%-1% Chinese prickly ash One or two or more of them, and the above calculations are based on the weight of fresh vegetables washed and drained.
作为优选方案,在步骤C中,泡菜液按重量百分比计由:1%-5%食盐、0.05%-0.15%泡菜专用菌粉剂、1%-8%糖、1%-8%白酒、0.1%-0.3%食品级氯化钙、余量为水混合配制而成。As a preferred version, in step C, the pickle liquid consists of: 1%-5% salt, 0.05%-0.15% pickle special bacteria powder, 1%-8% sugar, 1%-8% liquor, 0.1% -0.3% food grade calcium chloride, the balance is mixed with water.
在腌渍泡菜时加入食品级氯化钙可以保持发酵蔬菜的脆度。Adding food-grade calcium chloride when pickling kimchi preserves the crispness of fermented vegetables.
泡菜液反复使用,以增加泡菜产品的风味,同时可以减少接种量、缩短发酵周期。The kimchi liquid is used repeatedly to increase the flavor of the kimchi product, while reducing the amount of inoculation and shortening the fermentation period.
作为优选方案,所述泡菜液中各组分的重量百分比为:2%-3%的食盐、0.05%-0.15%的泡菜专用菌粉剂、3%-5%的糖、2%-6%的白酒、0.2%的食品级氯化钙、余量为水。As a preferred version, the percentage by weight of each component in the pickle liquid is: 2%-3% salt, 0.05%-0.15% special bacteria powder for pickle, 3%-5% sugar, 2%-6% Liquor, 0.2% food-grade calcium chloride, and the balance being water.
作为优选方案,所述泡菜专用菌粉剂按重量比为,乳酸菌:酵母菌:芽孢杆菌=7:2:1的复合菌剂。As a preferred solution, the special bacteria powder for pickles is a composite bacteria agent in a weight ratio of lactic acid bacteria: yeast: bacillus = 7:2:1.
泡菜在发酵过程中,采用乳酸菌、酵母菌、芽孢杆菌混种发酵,通过芽孢杆菌造成厌氧环境,加速以乳酸菌发酵为主导的厌氧发酵过程,芽孢杆菌能产生许多消化酶,利于降解蔬菜中的复杂碳水化合物,能产生抑菌素抑制杂菌生长;酵母菌在乳酸发酵结束后可以利用可发酵糖抑制腐败菌生长,在贮藏期间发生酯化反应,对盐渍蔬菜风味物质的形成有重要作用。During the fermentation process of kimchi, lactic acid bacteria, yeast, and bacillus are used for mixed fermentation. Bacillus creates an anaerobic environment and accelerates the anaerobic fermentation process dominated by lactic acid bacteria fermentation. Bacillus can produce many digestive enzymes, which is beneficial to the degradation of vegetables. complex carbohydrates, which can produce bacteriostatin to inhibit the growth of miscellaneous bacteria; yeast can use fermentable sugars to inhibit the growth of spoilage bacteria after lactic acid fermentation, and esterification reactions occur during storage, which is important for the formation of flavor substances in salted vegetables effect.
作为优选方案,所述步骤C中,糖类为葡萄糖、蔗糖或者冰糖。As a preferred solution, in the step C, the sugar is glucose, sucrose or crystal sugar.
作为优选方案,所述步骤D中,作为优选方案,发酵温度为20℃-30℃,发酵时间为48h-120h。As a preferred solution, in the step D, as a preferred solution, the fermentation temperature is 20°C-30°C, and the fermentation time is 48h-120h.
作为优选方案,在步骤D中,泡菜盐度保持在2%-6%,总酸度保持在0.3%-1%,即pH值保持在pH2-pH4.5。As a preferred solution, in step D, the salinity of pickles is kept at 2%-6%, and the total acidity is kept at 0.3%-1%, that is, the pH value is kept at pH2-pH4.5.
本发明的有益效果:Beneficial effects of the present invention:
1、利用人工接种发酵技术、在恒定温度下制备泡菜,发酵周期大大缩短。1. Using artificial inoculation fermentation technology to prepare kimchi at a constant temperature, the fermentation cycle is greatly shortened.
2、采用泡菜专用复合菌剂、促进了益生菌繁殖,酵母菌既可适度生长形成独特风味,又不至于过度繁殖在泡菜发酵液表面生花形成白色菌斑。2. The special compound bacterial agent for kimchi is used to promote the reproduction of probiotics. The yeast can grow moderately to form a unique flavor, but it will not over-proliferate and form white plaques on the surface of the kimchi fermentation liquid.
3、发酵过程中食盐用量减少、温度高,“亚硝峰”出现早且峰值低,加之乳酸菌的大量繁殖可以分解部分亚硝酸盐从而降低了发酵泡菜制品中食盐和亚硝酸盐的含量,提高了泡菜生产的安全性,满足了消费者对健康食品的追求。3. During the fermentation process, the amount of salt used is reduced, the temperature is high, and the "nitrite peak" appears early and the peak is low. In addition, the large-scale reproduction of lactic acid bacteria can decompose part of nitrite, thereby reducing the content of salt and nitrite in fermented kimchi products, and improving It ensures the safety of kimchi production and satisfies consumers' pursuit of healthy food.
4、由于益生菌的自身生长特性及周围环境因素的影响,导致进入市场的产品中活菌含量很低,而本专利中含有活益生菌数量较高,含量大于107cfu/kg,发酵液可重复使用,发酵风味浓郁,生产工艺简单,适合工业化生产,每批产品质量稳定。4. Due to the growth characteristics of probiotics and the influence of surrounding environmental factors, the content of live bacteria in products entering the market is very low, but the number of live probiotics contained in this patent is relatively high, with a content greater than 10 7 cfu/kg. Reusable, strong fermentation flavor, simple production process, suitable for industrial production, and stable quality of each batch of products.
具体实施方式Detailed ways
下面将结合本具体实施方式,对本发明做进一步的阐述。The present invention will be further described below in conjunction with the specific implementation manner.
实施例1Example 1
一种含益生菌的低盐不生花泡菜的制备方法,包括以下步骤:A preparation method of low-salt flower-free kimchi containing probiotics, comprising the following steps:
A、挑选新鲜、质嫩、无病虫害的新鲜蔬菜,洗净、沥干、切分,切成自然长度为4cm的小段;A. Select fresh, tender, and pest-free fresh vegetables, wash, drain, and cut into small pieces with a natural length of 4cm;
B、将切分好的蔬菜装入发酵坛中,压实;B. Put the cut vegetables into the fermentation jar and compact them;
C、向发酵坛中加入香辛料及蔬菜重量2.5倍的泡菜液,使其没过待发酵蔬菜;C, add spices and the kimchi liquid of 2.5 times of vegetables weight in the fermenting altar, make it submerge the vegetables to be fermented;
泡菜液由按重量比计:5%的食盐、0.15%的泡菜专用菌粉剂、5%糖、1%白酒、0.1%食品级氯化钙、余量为水配制而成;香辛料由按重量百分比:4%的大蒜、2%的生姜、0.8%的八角、0.8%的桂皮和0.4%的花椒混合而成;The pickle liquid is prepared by weight ratio: 5% salt, 0.15% special bacteria powder for pickle, 5% sugar, 1% white wine, 0.1% food grade calcium chloride, and the balance is water; the spice is prepared by weight percentage : 4% garlic, 2% ginger, 0.8% star anise, 0.8% cinnamon and 0.4% pepper;
所述泡菜液中的泡菜专用菌粉剂为乳酸菌:酵母菌:芽孢杆菌=7:2:1的复合菌剂;糖为葡萄糖、冰糖或蔗糖;The special bacterial powder of pickled vegetables in the described pickled vegetables liquid is the composite microbial agent of lactic acid bacteria: saccharomycete: bacillus=7:2:1; Sugar is glucose, rock sugar or sucrose;
D、装坛完成后,密封发酵坛,水封坛盖,保证发酵坛内厌氧环境;泡菜盐度保持在2%-6%,总酸度保持在0.3%-1%;D. After the altar is installed, seal the fermentation altar and seal the altar cover with water to ensure an anaerobic environment in the fermentation altar; the salinity of kimchi is kept at 2%-6%, and the total acidity is kept at 0.3%-1%;
E、将发酵坛置于自动控温的恒温发酵室内,在28℃下厌氧发酵120h,制得所需泡菜。E. Place the fermentation jar in a constant temperature fermentation room with automatic temperature control, and perform anaerobic fermentation at 28° C. for 120 hours to obtain the required kimchi.
实施例2Example 2
一种含益生菌的低盐不生花泡菜的制备方法,包括以下步骤:A preparation method of low-salt flower-free kimchi containing probiotics, comprising the following steps:
A、挑选新鲜、质嫩、无病虫害的新鲜蔬菜,洗净、沥干、切分,切成4cm长的小段;A. Select fresh, tender, and pest-free fresh vegetables, wash, drain, slice, and cut into 4cm-long pieces;
B、将切分好的蔬菜装入发酵坛中,压实;B. Put the cut vegetables into the fermentation jar and compact them;
C、向发酵坛中加入香辛料及蔬菜重量2倍的泡菜液,使其没过待发酵蔬菜;C, add spices and the kimchi liquid of 2 times of vegetable weight in the fermenting altar, make it submerge the vegetables to be fermented;
泡菜液由按重量比计:3%的食盐、0.12%的泡菜专用菌粉剂、4%的糖、1.5%的白酒、0.3%的食品级氯化钙、余量为水配制而成;The pickle liquid is prepared by weight ratio: 3% salt, 0.12% special bacteria powder for pickle, 4% sugar, 1.5% white wine, 0.3% food grade calcium chloride, and the balance is water;
香辛料由按重量百分比:4%的生姜、2%大蒜、0.8%的桂皮和0.4%的花椒混合而成;The spices are mixed by weight percentage: 4% ginger, 2% garlic, 0.8% cinnamon and 0.4% prickly ash;
所述泡菜液中的泡菜专用菌粉剂为乳酸菌:酵母菌:芽孢杆菌=7:2:1的复合菌剂;糖为蔗糖;The special bacteria powder for pickles in the pickles liquid is lactic acid bacteria: saccharomyces: the composite bacteria agent of bacillus=7:2:1; Sugar is sucrose;
D、装坛完成后,密封发酵坛,用食盐水封坛盖,保证发酵坛内厌氧环境;泡菜盐度保持在2%-6%,总酸度保持在0.3%-1%;D, after loading the altar, seal the fermentation altar, seal the altar cover with salt water, and ensure the anaerobic environment in the fermentation altar; the salinity of kimchi remains at 2%-6%, and the total acidity remains at 0.3%-1%;
E、将发酵坛置于自动控温的恒温发酵室内,在30℃下厌氧发酵96h,制得所需泡菜。E. Place the fermentation jar in a thermostatic fermentation room with automatic temperature control, and perform anaerobic fermentation at 30° C. for 96 hours to obtain the required kimchi.
实施例3Example 3
一种含益生菌的低盐不生花泡菜的制备方法,包括以下步骤:A preparation method of low-salt flower-free kimchi containing probiotics, comprising the following steps:
A、挑选新鲜、质嫩、无病虫害的新鲜蔬菜,洗净、沥干、切分,切成4cm长的小段;A. Select fresh, tender, and pest-free fresh vegetables, wash, drain, slice, and cut into 4cm-long pieces;
B、将切分好的蔬菜装入发酵坛中,压实;B. Put the cut vegetables into the fermentation jar and compact them;
C、向发酵坛中加入香辛料及蔬菜重量2倍的泡菜液,使其没过待发酵蔬菜;C, add spices and the kimchi liquid of 2 times of vegetable weight in the fermenting altar, make it submerge the vegetables to be fermented;
泡菜液由按重量比计:5%的食盐、0.08%的泡菜专用菌粉剂、8%的糖、1%的白酒、0.2%的食品级氯化钙、余量为水配制而成;The pickle liquid is prepared by weight ratio: 5% salt, 0.08% special bacteria powder for pickle, 8% sugar, 1% white wine, 0.2% food grade calcium chloride, and the balance is water;
香辛料由按重量百分比:4%的大蒜、2%生姜、0.8%的桂皮和0.4%的花椒混合而成;The spices are mixed by weight percentage: 4% garlic, 2% ginger, 0.8% cinnamon and 0.4% prickly ash;
所述泡菜液中的泡菜专用菌粉剂为乳酸菌:酵母菌:芽孢杆菌=7:2:1的复合菌剂;糖为冰糖;The special bacteria powder for pickles in the described pickles liquid is lactic acid bacteria: saccharomyces: the composite bacteria agent of bacillus=7:2:1; Sugar is rock sugar;
D、装坛完成后,密封发酵坛,用食盐水封坛盖,保证发酵坛内厌氧环境;泡菜盐度保持在2%-6%,总酸度保持在0.3%-1%;D, after loading the altar, seal the fermentation altar, seal the altar cover with salt water, and ensure the anaerobic environment in the fermentation altar; the salinity of kimchi remains at 2%-6%, and the total acidity remains at 0.3%-1%;
E、将发酵坛置于自动控温的恒温发酵室内,在30℃下厌氧发酵96h,制得所需泡菜。E. Place the fermentation jar in a thermostatic fermentation room with automatic temperature control, and perform anaerobic fermentation at 30° C. for 96 hours to obtain the required kimchi.
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