CN104818234A - Streptomycete with cadmium tolerant characteristic and application thereof - Google Patents

Abstract

The present invention aims at the poor adaptability of microorganisms to polluted soil, low compatibility with plants, and low survival rate of microorganisms in the prior art, and screens out a cadmium-resistant bacterium, and aims at the restoration method of contaminated soil, which belongs to the treatment of heavy metal pollution technology field. The strain has strong adaptability to polluted soil, can grow symbiotically with plants, and has a high survival rate. The strain itself has a strong ability to enrich heavy metal cadmium, has a strong ability to repair polluted soil, and reduces the damage to the environment caused by cadmium pollution.

Description

A Strain of Streptomyces with Cadmium Resistance and Its Application

Technical field

The invention relates to a Streptomyces strain with cadmium-resistant properties and a method for repairing heavy metal-polluted environments, belonging to the technical field of heavy metal pollution control.

Background technique

Cadmium is one of the most toxic heavy metal elements, which is extremely harmful. Excessive cadmium in the soil will inhibit the normal growth of plants, and the residue in the edible part will also affect human health through the food chain. In recent years, cadmium pollution incidents have occurred frequently, such as Beijiang in Guangdong, Longjiang in Guangxi, Zhuzhou in Hunan, Liuyang in Hunan, and cadmium-contaminated rice in Japan are shocking. The treatment of cadmium-contaminated soil has also aroused widespread concern at home and abroad.

The use of engineering, physical and chemical methods to remediate cadmium-contaminated soil has certain limitations, is difficult to deal with on a large scale, and will lead to the destruction of soil structure, the decline of biological activity and the degradation of soil fertility. The cycle of agricultural ecological measures is too long, and the effect is not significant. At present, there are mainly three ways to remediate heavy metal-contaminated soil: physical remediation, chemical remediation, and biological remediation. Traditional physical techniques mainly include ion exchange method, adsorption method, etc. These methods have good effects on small-area polluted soil, but they are easy to cause secondary pollution, destroy the structure and fertility of the soil, and the cost is high; chemical remediation technology has Chemical precipitation method, electrochemical reduction method, redox method, etc. Although these technologies are simple and effective, they do not fundamentally remove heavy metals in the soil, but only change their existing forms, which are easy to activate again. It has a certain destructive effect on the ecological environment.

Bioremediation is the enrichment or extraction of heavy metals in soil by using the metabolic activities of microorganisms or plants. Bioremediation technologies mainly include phytoremediation and microbial remediation. Phytoremediation refers to the general term for a class of technologies that use plants to absorb heavy metals in the soil, and finally achieve the removal of heavy metals in the soil. Phytoremediation has the advantages of low cost, green purification, no damage to the soil ecological environment, and no need for secondary treatment. It is the future The main development direction of restoration technology. The plants used for restoration can include all plants of the higher plant kingdom, such as wild grasses, weeds, and cultivated trees, turf and crops. Among them, there is a class of plants whose heavy metal absorption is significantly higher than that of other common plants, that is, hyperaccumulators. Celosia argentea L. is a plant that has a certain ability to enrich heavy metals and cadmium. Under natural conditions, the cadmium content in the plant can reach more than 50mg/kg. It grows rapidly, and its seeds are easy to germinate and have certain Ornamental, is a good soil heavy metal restoration plant resources.

However, phytoremediation also has certain defects, such as: heavy metal-enriched plants usually have low biomass and slow growth; they are selective to metals and are not suitable for the treatment of multiple heavy metal pollution; the absorption capacity of plants to heavy metals is often limited by soil Environmental and climatic conditions; in addition, pollutants may return to the soil through fallen leaves. These have limited the application of phytoremediation technology in the treatment of heavy metal contaminated soil. In order to, researchers consider adopting many ways to make up for its inadequacy.

In recent years, the use of heavy metal-resistant microorganisms to enhance the absorption of heavy metals by plants has gradually become a research hotspot at home and abroad. Microorganisms enhance the absorption of heavy metals by plants. Plant stress resistance, by means of increasing biomass to improve repair ability. For example, mycorrhizal fungi can dissolve and transport inorganic mineral elements in the soil; rhizobia carry out biological nitrogen fixation to promote plant nutrient absorption; the stable root microenvironment formed by the existence of various microorganisms in the root enhances the stress resistance of plants, etc., all of which directly Or indirectly increase the biomass of plants and improve the ability of phytoremediation. The second is to increase the concentration of heavy metals in plant roots and promote the absorption or fixation of heavy metals. Microorganisms not only adsorb heavy metals through their own components such as mycorrhizal mycelium, chitin, pigments, EPS, etc., but also activate heavy metals through various organic acids or special substances secreted by them, increasing their concentration in plant roots. . The heavy metals accumulated in the roots of plants are transported to the plants through the proteins that have a translocation function for specific heavy metals in certain plants; or the heavy metals in the soil are adsorbed on the rhizosphere, reducing their mobility, thereby reducing their entry into the food chain possibility, and reduce the concentration of heavy metals in the soil when removing plants, so as to achieve the purpose of plant absorption and fixation. Therefore, it is a feasible way to inoculate the rhizosphere with cadmium-tolerant microorganisms to promote the absorption capacity of cadmium and strengthen its soil remediation effect, which has great potential for development.

The Chinese patent with publication number CN103551379A discloses a method for ecological restoration in cadmium-contaminated soil by using cadmium-tolerant fungi, which involves microbial ecological restoration technology. The method comprises: ① separating and purifying the cadmium-resistant fungi; ② identifying the cadmium-resistant fungi; ③ determining the optimum growth conditions for the cadmium-resistant fungi; ④ preparing a restoration matrix for the cadmium-resistant fungi; The present invention uses the rhizosphere soil of dominant plants in cadmium-polluted areas as the source of cadmium-resistant microorganism screening, and overcomes the adaptability of the screened cadmium-resistant microorganisms to the soil environment of the restoration site; The cadmium fungus has compatible bermudagrass, which overcomes the repulsion between plants and microorganisms; the cadmium-tolerant fungi are inoculated into sawdust for application, which ensures the nutrient supply for microbial growth and effectively utilizes waste; it is low in cost and easy to operate. Microorganisms grow rapidly and can reproduce on a large scale.

The Chinese patent with publication number CN103350105A discloses a method for plant-microbe joint enrichment of heavy metal cadmium in soil. First, cadmium-enriched plants are planted in cadmium-contaminated soil, and then cadmium-tolerant bacteria are inoculated in the rhizosphere soil of cadmium-enriched plants. Routine cultivation, watering, moisturizing, using cadmium-enriched plants to absorb cadmium and cadmium-tolerant bacteria to strengthen the enrichment of cadmium in soil, repeating the above process can achieve the purpose of repairing cadmium-contaminated soil. The soil restoration method of the invention has good effect, low cost, easy management and operation, no secondary pollution, and wide application prospect.

The Chinese patent with the publication number CN103952333A discloses a heavy metal cadmium resistant bacterium and a method for inhibiting crops from absorbing heavy metal cadmium, belonging to the technical field of agriculture and environmental pollution control. The bacterial strain is Pseudomonas sp. TCd-1, which was registered and preserved in the Chinese Type Culture Collection Center of Wuhan University on December 11, 2013, and the strain preservation number is CCTCC M2013649. The strain TCd-1 was identified as a Pseudomonas sp. bacteria, which is resistant to various heavy metals, and the resistant concentration to cadmium, lead and nickel reaches 900mg·L-1, 800mg·L-1 and 600mg respectively ·L-1. The effective number of viable bacteria in the bacterial liquid is about 1.85-1.87×109/ml. Adding the bacterial solution to moist soil polluted by heavy metal cadmium, adding 2mL of the bacterial solution per kilogram of soil, can restore the inhibitory effect of cadmium on rice growth, and effectively reduce the cadmium content in various parts of rice, especially brown rice.

The Chinese patent with publication number CN101955925A discloses a method for multi-parental fusion of eukaryotic and prokaryotic microbial protoplasts based on genome shuffling technology. The technical scheme adopted for realizing the object of the present invention is: firstly carry out artificial mutagenesis to microorganisms, obtain the mutant microorganisms with efficient anti-cadmium performance to cadmium through screening; Cell protoplast multi-parental fusion method, through culturing slant spores, strain mutagenesis, genome shuffling, screening, and recursive multiple fusion, to obtain a strain with good characteristics of cadmium resistance. Using the cell protoplast multi-parent fusion method established by the present invention, the cadmium-resistant Bacillus subtilis and Candida tropicalis are transformed, and the cadmium-resistant performance of two strains is greatly improved compared with the original Bacillus subtilis and Candida tropicalis excellent fusion strain.

The Chinese patent with the publication number CN102513340A discloses a method for remediating heavy metal cadmium-contaminated soil, which is characterized in that the plant used for remediating heavy metal cadmium is the cadmium-tolerant variety Tobacco K326, and the soil chelation inducer used is chitosan Hydrochloric acid solution to activate heavy metal cadmium in the soil is a bacterial strain isolated by enrichment and screening in this laboratory. The three work together to remediate heavy metal cadmium-contaminated soil; Transplant after the leaf-core stage; add chitosan before transplanting plants; carry out mass fermentation and culture of the isolated, screened and purified strains before transplanting plants, and add heavy metal cadmium in the long logarithmic phase to the soil for activation The strains are mixed with soil for later use; the tobacco varieties can be managed regularly after transplanting. The method adopts a combined restoration method of plants and microorganisms, and a certain amount of microorganisms that activate heavy metals are added to the soil to work together to improve the restoration efficiency, thereby improving the restoration efficiency of heavy metal-contaminated soil.

The above data show that microbial remediation has the following advantages: it does not destroy the soil environment for plant growth; it does not form secondary pollution or cause the transfer of pollutants; it can degrade pollutants in situ to the greatest extent; it is low in cost; Operators are directly affected by pollutants; high efficiency, short repair time, and less interference to the surrounding environment. At present, microorganisms also have the following deficiencies in remediating heavy metal-contaminated soils: the adaptability of microorganisms to contaminated soil is poor; the compatibility between microorganisms and plants is low; and the survival rate of microorganisms is low.

Contents of the invention

The purpose of the present invention is to overcome the shortcomings and deficiencies of the prior art, and to provide a method for ecological restoration in a cadmium-polluted environment by using cadmium-tolerant actinomycetes.

The heavy metal cadmium-tolerant actinomycete described in the present invention is Streptomyces sp. CdTB01 bacterial strain, was preserved on December 23, 2014 at the Chinese Academy of Microbiology Research Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing, China The General Microorganism Center of the Preservation Management Committee, the deposit number is CGMCC No.10234.

One of the contents of the invention. The actinomycetes with strong cadmium tolerance were isolated from the mixed soil samples of Fenghuang County, Guzhang County and Luxi County in Xiangxi, Hunan Province. After morphological, physiological, biochemical and molecular identification, they were identified as chain Mold (Streptomyces sp.). The strain has different culture characteristics on different media. No soluble pigments are produced on most media, no aerial hyphae, and the hyphae in the base are mostly yellowish-white. The main biological characteristics are: H ₂ S production, starch hydrolysis is positive; gelatin liquefaction, cellulose hydrolysis, milk coagulation and liquefaction are negative. CdTB01 strain can utilize sucrose, glucose, fructose and D-mannose well, but cannot utilize arabinose.

The medium involved in the present invention is as follows: LB solid medium consists of 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, 12 g of agar, 1000 mL of distilled water, and sterilized at 121° C. for 20 minutes. The components of the separation medium are 1mmol/L CdCl ₂ (cadmium chloride), 5mmol/L CdCl ₂ , 10mmol/LCdCl ₂ , 20mmol/L CdCl ₂ , 30mmol/L CdCl ₂ , 40mmol/L CdCl ₂ , 50mmol/L CdCl ₂ , 55mmol/L CdCl ₂ , 60mmol/L CdCl ₂ LB solid medium, sterilized at 121°C for 20min. The activation medium adopts PDA (sucrose) medium, and its composition is 200g of peeled potatoes, 20g of sucrose, 1000ml of water, natural pH, and sterilized at 115°C for 30min.

Among them, the screening process of Streptomyces: From the mixed soil samples in Fenghuang County, Guzhang County, and Luxi County, Xiangxi County, Hunan Province, the separation medium was used to screen, and multiple strains were screened, and the cadmium tolerance was passed. Select as the main index to obtain a strain with high cadmium resistance, and the result of 16S rDNA gene sequence determination is shown in SEQ ID No: 3. According to the data analysis of culture characteristics, microscopic characteristics, rRNA gene sequence and other data analysis of the selected strain, it was identified as Streptomyces sp.

The second content of the invention provides the preparation of the Streptomyces sp. CdTB01 bacterial agent and its application in cadmium-polluted environment.

The second object of the present invention is the use of Streptomyces for rapidly enriching cadmium in a cadmium-polluted environment.

The uses described therein include: the process of using streptomyces starter (solid preparation) to mix material (cadmium-contaminated soil) and the process of directly treating cadmium-contaminated wastewater with streptomyces inoculum.

In a specific embodiment, the above-mentioned Streptomyces starter (solid powder) is used to add an appropriate amount of sterile water to dilute, and to inoculate the treatment process of cadmium-contaminated soil by spraying. It can quickly ferment and enrich the heavy metal cadmium. Compared with the traditional treatment process, it can shorten the treatment cycle by more than half, and at the same time reduce the chance of re-pollution of cadmium pollution and ensure the success rate of cadmium pollution restoration. In addition, the prepared Streptomyces inoculum can also be added to the cadmium-containing wastewater to fully stir and adsorb, so as to quickly remove the heavy metal cadmium in the water body.

Among them, using Streptomyces fermentation broth (cadmium-contaminated soil) repair process: use Streptomyces fermentation agent (solid powder), the number of microorganisms per gram is more than 1×10 ¹⁰ cfu, first dilute the powder with sterile water, spray Inoculate on the surface of cadmium-contaminated soil, and carry out direct repair treatment after inoculation. The dosage is 0.5kg-2.5kg of streptomyces powder per mu of cadmium-contaminated soil.

Streptomyces inoculum repair cadmium-polluted water treatment process: Streptomyces inoculum is added to cadmium-contaminated wastewater, stirred to make it fully contact with cadmium ions in the wastewater and adsorbed on the surface of the inoculum, so as to realize the purification treatment of cadmium-contaminated wastewater.

Description of drawings

Figure 1: The clear circle produced by Streptomyces sp. CdTB01 after growth in cadmium-containing medium. 1 and 2 are medium containing CdCl ₂ and Cd(NO ₃ ) ₂ respectively.

Figure 2: Microscopic observation results of Streptomyces sp. CdTB01.

technical effect

1. The strain has strong adaptability to polluted soil, can co-exist well with plants, and has a high survival rate.

2. The strain itself has a strong ability to enrich heavy metal cadmium, has a strong ability to repair polluted soil, and has a strong ability to remove cadmium in polluted wastewater, reducing the damage of cadmium pollution to the environment.

Detailed ways

Hereinafter, the present invention will be further described with examples, but it is not limited to any one of these examples or similar examples.

Example 1: Screening of bacterial strain CdTB01

Take 10g of mixed soil samples from Fenghuang County, Guzhang County and Luxi County in Xiangxi, Hunan Province, put them into a conical flask, add 100mL of sterile water, shake at room temperature for 30min, and take 0.1mL of the supernatant after standing for 10min to dilute to 10 ^-5 were respectively spread on solid separation culture plates containing 1-60mmol/L CdCl ₂ , cultured at a constant temperature of 30°C, and a total of 11 bacterial strains with different degrees of cadmium resistance effects were screened out. Further, a bacterial strain that can tolerate 55mmol/L CdCl ₂ or Cd(NO ₃ ) ₂ was obtained through repeated verification and screening. After growing on a medium containing Cd, the bacterial strain can produce a transparent circle around the bacteria, indicating that This strain may have the function of adsorbing Cd. And the strain can tolerate 2mmol/L Pb(NO ₃ ) ₂ and 3mmol/L NiCl ₂ .

Embodiment 2: bacterial strain identification

1. Morphological and biochemical identification

1. The form is Streptomyces. Under the optical microscope, the mycelium of CdTB01 has many and dense branches, and spores are formed, as shown in Figure 2.

2. The culture characteristics of strain CdTB01 are as follows:

      

      

3. According to the method described in the "Common Bacteria System Identification Manual", some physiological and biochemical indicators and carbon source utilization of the strain CdTB01 were studied, and the results are as follows:

      

Note: + means use, ++ means better use, - means no use.

2. Molecular identification

1. Design and select appropriate primers

According to the 16S rDNA gene sequence of actinomycetes, design PCR primers, respectively:

SEQ ID No: 1 5-AGAGTTTGATCCTGGCTCAG-3 (SEQ ID No: 1) SEQ ID No: 2 5-GGTTACCTTGTTACGACTT-3 (SEQ ID No: 2)

Related primers were synthesized at Sangon Bioengineering (Shanghai) Co., Ltd.

2. Universal primer amplification

For the DNA of Streptomyces, ABI 9700 PCR instrument was used for biological experiments.

(1) The reaction system of PCR is:

PCR reaction buffer (10x PCR Buffer with 20mM MgCl ₂ ) 2.5μL dNTP mix (25mM each) 1μL High-fidelity Taq enzyme (PCR Enzyme, 5U/μL) 1μL SEQ ID No: 1 (1 μM) 1μL SEQ ID No: 2 (1 μM) 1μL Actinomycetes DNA 1μL Ultra-pure water 17.5μL

The above reagents, except actinomycetes DNA, ultrapure water, and primers, were all from Sequenom, USA.

(2) The cycle parameters of PCR are: 95°C, 4 minutes, 1 cycle, 95°C, 20 seconds, 56°C, 30 seconds, 72°C, 60 seconds, 45 cycles, 72°C, 3 minutes, 1 cycle , 4 ℃, save.

3. The PCR amplification product was sequenced by Sangon Bioengineering (Shanghai) Co., Ltd., and the 16S rDNA sequencing result of Streptomyces CdTB01 was obtained: see SEQ ID No: 3.

Example 3: Cadmium uptake properties of bacterial strain CdTB01

Prepare LB liquid medium containing 4mmol/L CdCl ₂ (the composition of LB liquid medium is peptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, sterilized at 121°C for 20min.), each bottle is 400mL, 121°C Sterilize for 20 minutes. Inoculate 1mL of CdTB01 bacterial liquid, culture it on a shaking table at 30°C and 200rpm for 3 days, take 100mL of fermentation broth, and centrifuge at 8000rpm for 10min to obtain the supernatant, which is digested and measured by atomic absorption spectrometer. The cadmium content is 216mg/L , the cadmium content dropped from 448mg/L to 216mg/L, and the removal rate reached over 50%.

Embodiment 4: The restoration effect of bacterial strain CdTB01 on plant growth under cadmium stress

Take rice seedlings of 15 cm, and evenly plant them in soil containing 5 mg·L ^-1 cadmium chloride (potted in a 20L nutrient pot), plant 4 rice seedlings in each pot, and plant them in the soil without adding cadmium chloride As a control, two treatments of adding 500ml of distilled water and 500ml of CdTB01 bacterial solution were set, and each treatment was repeated 3 times. Each treatment was placed in a greenhouse at 25°C for light cultivation. Water was added quantitatively every day, and rice biomass was measured after 20 days. The results showed that the biomass of rice treated with cadmium-containing soil by adding CdTB01 bacterial solution was very similar to that of rice without cadmium. The height increased by 25.6%, the fresh weight increased by 42.7%, the cadmium content in the whole rice plant decreased by 50%, and the restoration effect was very remarkable.

Embodiment 5: the cadmium adsorption effect of bacterial strain CdTB01 bacterial agent

Take 200mL of CdTB01 bacteria liquid in the stable growth period, centrifuge at 8000rpm at room temperature for 10 minutes to collect the bacteria, dry and grind at 105°C to form dry bacteria powder. Prepare physiological saline with cadmium concentrations of 100mg/L, 500mg/L, 900mg/L, 1300mg/L, and 1700mg/L respectively, weigh 0.1g of dry bacteria powder to treat cadmium-containing physiological saline for 1 hour, and centrifuge at 8000rpm for 10min to obtain Clear liquid, adopt atomic absorption spectrometer to measure its cadmium content after digesting, and measuring result is as follows:

Cadmium concentration in initial solution (mg/L) Concentration of cadmium in supernatant after treatment (mg/L) Adsorption rate of CdTB01 dry bacterial powder 100 2.69 97.31% 500 11.70 97.66% 900 25.32 97.19% 1300 65.27 96.16% 1700 68.44 94.74%

The adsorption rate of CdTB01 dry bacterial powder decreases with the increase of cadmium concentration in the solution, but in the environment with high cadmium concentration, the adsorption rate can still be maintained above 94%, indicating that the bacteria has a significant effect on the restoration of cadmium-contaminated wastewater. Effect.

Sequence of SEQ ID No: 3:

5’-GGAGAACTAGTATAGGGCGATTGGGCCCGACGTCGCATGCTCCCGGCCGCCATGGCCGCGGGATTGGTTACCTTGTTACGACTTCGTCCCAATCGCCAGTCCCACCTTCGACAGCTCCCTCCCACAAGGGGTTGGGCCACCGGCTTCGGGTGTTACCGACTTTCGTGACGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGACTCCGACTTCATGGGGTCGAGTTGCAGACCCCAATCCGAACTGAGACCGGCTTTTTGAGATTCGCTCCACCTCACGGTATCGCAGCTCATTGTACCGGCCATTGTAGCACGTGTGCAGCCCAAGACATAAGGGGCATGATGACTTGACGTCGTCCCCACCTTCCTCCGAGTTGACCCCGGCGGTCTCCTGTGAGTCCCCATCACCCCGAAGGGCATGCTGGCAACACAGAACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCACCACCTGTACACCGACCACAAGGGGGCGCCCATCTCTGGACGTTTCCGGTGTATGTCAAGCCTTGGTAAGGTTCTTCGCGTTGCGTCGAATTAAGCCACATGCTCCGCCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTAGCCTTGCGGCCGTACTCCCCAGGCGGGGAACTTAATGCGTTAGCTGCGGCACCGACGACGTGGAATGTCGCCAACACCTAGTTCCCACCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATGGCCCAGAGATCCGCCTTCGCCACCGGTGTTCCTCCTGATATCTGCGCATTTCACCGCTACACCAGGAATTCCGATCTCCCCTACCACACTCTAGCTAGCCCGTATCGACTGCAGACCCGAGGTTAAGCCTCGGGCTTTCACAATCGACGTGACA AGCCGCCTACGAGCTCTTTACGCCCAATAATTCCGGACAACGCTTGCGCCCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGGCGCTTCTTCTGCAGGTACCGTCACTTTCGCTTCTTCCCTGCTGAAAGAGGTTTACAACCCGAAGGCCGTCATCCCTCACGCGGCGTCGCTGCATCAGGCTTTCGCCCATTGTGCAATATTCCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCCGGTCGCCCTCTCAGGCCGGCTACCCGTCGTCGCCTTGGTGAGCCATTACCTCACCAACAAGCTGATAGGCCGCGGGCTCATCCTTCACCGCCGGAGCTTTTAACCCTCGCAGATGCCTGCGAGAGTGTTATCCGGTATTAGACCCCGTTTCCAGGGCTTGTCCCAGAGTGAAGGGCAGATTGCCCACGTGTTACTCACCCGTTCGCCACTAATCCCCACCGAAGTGGTTCATCGTTCGACTTGCATGTGTTAAGCACGCCGCCAGCGTTCGTCCTGAGCCAGGATCAAACTCTAATCACTAGTGCGGCCGCCTGCAGGTCGACCATATGGGAGAGCTCCCAACGCGTTGGATGCATAGCTTGAGTATTCTATAGGCACCTAATGCAGGCCC-3’。

Claims (8)

1. The present invention provides an isolation, screening and identification comprising Streptomyces sp., wherein said bacterial strain has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on December 23, 2014, and the preservation number: CGMCC No .10234. 2. according to the method for claim 1, streptomyces also comprises: this bacterium culture substrate and this bacterium metabolite. 3. according to the method for claim 1, streptomyces is used for the purposes of fast enrichment cadmium in cadmium polluted environment. 4. The method according to claim 3, wherein the use of the Streptomyces comprises: a treatment process of mixing cadmium-contaminated soil with a Streptomyces solid-state fermentation preparation, and a process of treating cadmium-containing wastewater with a Streptomyces inoculant. 5. The method according to claim 4, wherein the application of the Streptomyces comprises: microbial expansion culture mechanism; management of cadmium-contaminated remediation soil; remediation treatment of cadmium-containing wastewater; product development management and quality control. 6. according to the method for claim 5, wherein said streptomyces solid fermentation powder adds appropriate amount of sterile water dilution, by the treatment process of spraying inoculation cadmium contaminated soil; 7. The method according to claim 6, wherein said Streptomyces fermented liquid is mixed with cadmium-contaminated soil remediation treatment process: adopt Streptomyces solid fermentation powder, each gram contains more than 1 × 1010 ^cfu of microorganisms, first use sterile water to dissolve the powder Dilute, spray and inoculate on the surface of cadmium-contaminated soil, and carry out direct repair treatment after inoculation. The dosage is 0.5kg-2.5kg of streptomyces powder per mu of cadmium-contaminated soil. 8. according to the method for claim 6, adopt streptomyces solid fermented powder to directly process the restoration process of cadmium polluted waste water: with streptomyces solid fermented powder, every gram containing more than 1 * 1010 ^cfu of microbial quantity, add in the cadmium polluted waste water, pass Stir to make it fully contact with cadmium ions in the wastewater and adsorb on the surface of the bacterial agent, so as to realize the purification treatment of cadmium-contaminated wastewater. The dosage is 1kg-2.5kg of Streptomyces powder per ton of cadmium-contaminated wastewater.