CN104807789A - High-sensitivity method for detecting content of heavy metal in water body - Google Patents
High-sensitivity method for detecting content of heavy metal in water body Download PDFInfo
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- CN104807789A CN104807789A CN201410852058.9A CN201410852058A CN104807789A CN 104807789 A CN104807789 A CN 104807789A CN 201410852058 A CN201410852058 A CN 201410852058A CN 104807789 A CN104807789 A CN 104807789A
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Abstract
The invention discloses a high-sensitivity method for detecting the content of heavy metal in a water body. The method comprises the following steps: stirring and mixing 50nM papain and a first activation mixed liquor to form Eu<3+>-modified papain, filtering the Eu<3+>-modified papain through a dialysis bag to obtain purified Eu<3+>-modified papain; stirring and mixing 50nM papain and a second activation mixed liquor to react under the condition of 20-30 DEG C so as to form fluorescent dye XL665-modified papain, wherein molar ratio of the fluorescent dye XL665 to papain is 1:5; and fully mixing the Eu<3+>-modified papain and the fluorescent dye XL665-modified papain according to the molar ratio of 1:1 to form a fluorescence detection liquid. The method provided by the invention has advantages of rapid speed, high sensitivity, wide linear range and low use cost. By the method, field test can be realized, and detection efficiency, timeliness and detection sensitivity of detecting heavy metal ions in wastewater are enhanced.
Description
Technical field
The present invention relates to water body testing environment field, be specifically related to a kind of detection method of high sensitivity heavy metal in water content.
Background technology
At present due to the heavy metal wastewater thereby discharge in exploitation of mineral resources, smelting processing, machine-building and the industry such as instrument and meter, organic synthesis, cause the water environment pollution much caused by heavy metal (as lead, mercury, cadmium, cobalt etc.).Heavy metal wastewater thereby is that environmental pollution is the most serious and endanger one of maximum industrial waste water to the mankind, and therefore lead, mercury, cadmium, the isoionic concentration of cobalt also become the important indicator of monitoring water environment.By detecting lead, mercury, cadmium, the isoionic concentration of cobalt in water body, thus roughly reflect the heavy metal pollution degree of water body.The standard method of analysis of traditional heavy metal monitoring, mainly based on spectrophotometric method and atomic absorption spectrophotometry, utilizes the specific absorption of measured matter in certain wave strong point or a wavelength range to carry out qualitative and quantitative analysis to this material.The continuous progress of science and technology at any time, the more additive method of domestic application also has atomic absorption method, atomic fluorescence method and the analytical approach such as more advanced inductively coupled plasma emission spectrography (ICP-AES), inductively coupled plasma mass spectrometry method (ICP-MS).Expensive for this type of main equipment, complicated operation, maintenance are large, cost is higher, not easily realize the drawbacks such as Site Detection.
Summary of the invention
The invention provides a kind of detection method of high sensitivity heavy metal in water content, this detection method has that speed is fast, highly sensitive, the range of linearity is wide, use cost is low, can realize Site Detection, improves heavy metal ions in wastewater detection efficiency, promptness and detection sensitivity.
For achieving the above object, the technical solution used in the present invention is: a kind of detection method of high sensitivity heavy metal in water content, comprises the following steps:
Step 1. will containing Eu
3+the N-hydroxy thiosuccinimide solution of the europium cryptate of ion and the EDC solution of 300nM and 300nM is uniformly mixed and activates 30min and form first and activate mixed liquor;
50nM papain and described first are activated mixed liquor and are uniformly mixed coupling reaction under the condition of 20 DEG C ~ 30 DEG C and form Eu by step 2.
3+the papain that salt is modified, described containing Eu
3+the europium cryptate of ion and the molar ratio of papain are 1:5, described containing Eu
3+the molar ratio of the europium cryptate of ion and EDC and N-hydroxy thiosuccinimide is 1:2 ~ 5, described Eu
3+the papain that salt is modified filters the Eu after obtaining purifying through bag filter
3+the papain that salt is modified;
The N-hydroxy thiosuccinimide solution of the EDC solution of fluorescent dye XL665 and 300nM and 300nM is uniformly mixed activation 30min formation second activation mixed liquor by step 3.;
50nM papain and described second are activated mixed liquor and are uniformly mixed coupling reaction under the condition of 20 DEG C ~ 30 DEG C and form the papain that fluorescent dye XL665 modifies by step 4., in described fluorescent dye XL665 and step 4, the molar ratio of papain is 1:5, and the molar ratio of described fluorescent dye XL665 and EDC and N-hydroxy thiosuccinimide is 1:2 ~ 5;
Step 5. is by described Eu
3+the papain that salt is modified and the papain that fluorescent dye XL665 modifies are thoroughly mixed to form fluoroscopic examination liquid, described Eu
3+the papain that salt is modified fully mixes according to 1:1 mol ratio with the papain that fluorescent dye XL665 modifies;
Described fluoroscopic examination liquid is added the excitation light source excites of warp after aqueous solution to be detected thus the fluorescent emission intensity detected at 620nm and 665nm wavelength by step 6., and calculates 665nm fluorescent emission intensity and 620nm fluorescent emission intensity ratio obtains liquid fluorescence ratio to be measured;
Step 7. is by liquid fluorescence ratio to be measured and standard fluorescence curve comparison thus obtain the concentration of heavy metal ion in aqueous solution to be detected, and described standard fluorescence curve is the fluorescence curve that the liquid fluorescence ratio obtained based on standard solution and the described fluoroscopic examination liquid of several known heavy metal concentration calibrates.
Further improvement project in technique scheme is as follows:
1., in such scheme, the papain that XL665 described in step 4 modifies filters the papain of the modification of the XL665 after obtaining purifying through bag filter.
2., in such scheme, described heavy metal comprises Hg
2+, Cu
2+, Pb
2+.
3., in such scheme, the stir speed (S.S.) of described step 1 ~ 4 is 300rpm.
4., in such scheme, the optical source wavelength that in described step 6, excitation source produces is 360nm.
5., in such scheme, in described step 2, step 4, the time of coupling reaction is 2 h.
6., in such scheme, in described step 2, step 4, the temperature of coupling reaction is 25 DEG C.
Because technique scheme is used, the present invention compared with prior art has following advantages and effect:
1. the detection method of high sensitivity heavy metal in water content of the present invention, papain, Eu that its Heavy Metals in Waters ion to be detected makes fluorescent dye XL665 modify
3+the papain that salt is modified is assembled, thus the distance furthered between the two, cause two fluorophors close to time produce the characteristic of the Fluorescence Resonance Energy transfer between Eu3+ salt and XL665, discharged by the portion of energy that cryptate is caught when exciting, emission wavelength is 620nm; Another part energy trasfer is to XL665 or fluorescent dye d2, and emission wavelength is 665nm, thus reaches the object detected the heavy metal substance in water body environment, improves heavy metal ions in wastewater detection efficiency, promptness and detection sensitivity.
2. the detection method of high sensitivity heavy metal in water content of the present invention, Eu
3+the molar ratio of salt and papain is 1:5, Eu
3+the ratio of salt and EDC solution and N-hydroxy thiosuccinimide solution-3-ethyl carbodiimide solution is that this ratio in 1:2 ~ 5 fully can activate Eu
3+salt surface amino groups group, the excessive joint efficiency that can increase Eu3+ salt and papain of papain, both too can not waste reagent; Again can not because of affecting Eu adding of excessive activator
3+the properties of product of salt and fluorescent dye XL665; Secondly, EDC solution and N-hydroxy thiosuccinimide solution and Eu in the present invention
3+the mol ratio of salt is 2 ~ 5:1, and the mol ratio of the N-hydroxy thiosuccinimide of fluorescent dye XL665 and EDC solution and 300nM is 1:2 ~ 5, can activate Eu
3+salt surface amino groups group, thus carry out coupling with the carboxylic group on papain surface; Secondly; In described scheme, stir speed (S.S.) is 300rpm, and each material can be made fully to react, and improves coupling efficiency, and the optical source wavelength that excitation source produces is 360nm, 360nm is Eu
3+again, the time selecting excellent coupling reaction is 2 h to the excitation wave of ion, makes to make each material fully react, and can not reduce each species activity again due to the reaction time of overlength.
Accompanying drawing explanation
Accompanying drawing 1 is reaction principle schematic diagram of the present invention;
Accompanying drawing 2 is typical curve calculating schematic diagram of the present invention;
Accompanying drawing 3 deposits the fluorescence spectrum figure obtained in case for heavy metal free ion;
Accompanying drawing 4 deposits the fluorescence spectrum figure obtained in case for heavy metal ion of the present invention.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described:
Embodiment: a kind of detection method of high sensitivity heavy metal in water content, comprises the following steps:
Step 1. will containing Eu
3+the N-hydroxy thiosuccinimide solution of the europium cryptate of ion and the EDC solution of 300nM and 300nM is uniformly mixed and activates 30min and form first and activate mixed liquor;
50nM papain and described first are activated mixed liquor and are uniformly mixed coupling reaction under the condition of 20 DEG C ~ 30 DEG C and form Eu by step 2.
3+the papain that salt is modified, described containing Eu
3+the europium cryptate of ion and the molar ratio of papain are 1:5, described containing Eu
3+the molar ratio of the europium cryptate of ion and EDC and N-hydroxy thiosuccinimide is 1:2 ~ 5; Eu described in step 2
3+the papain that salt is modified filters the Eu after obtaining purifying through bag filter
3+the papain that salt is modified;
The N-hydroxy thiosuccinimide solution of the EDC solution of fluorescent dye XL665 and 300nM and 300nM is uniformly mixed activation 30min formation second activation mixed liquor by step 3.;
50nM papain and described second are activated mixed liquor and are uniformly mixed coupling reaction under the condition of 20 DEG C ~ 30 DEG C and form the papain that fluorescent dye XL665 modifies by step 4., in described fluorescent dye XL665 and step 4, the molar ratio of papain is 1:5, and the molar ratio of described fluorescent dye XL665 and EDC and N-hydroxy thiosuccinimide is 1:2 ~ 5; The papain that XL665 described in step 4 modifies filters the papain of the modification of the XL665 after obtaining purifying through bag filter;
Step 5. is by described Eu
3+the papain that salt is modified and the papain that fluorescent dye XL665 modifies are thoroughly mixed to form fluoroscopic examination liquid, described Eu
3+the papain that salt is modified fully mixes according to 1:1 mol ratio with the papain that fluorescent dye XL665 modifies;
Described fluoroscopic examination liquid is added the excitation light source excites of warp after aqueous solution to be detected thus the fluorescent emission intensity detected at 620nm and 665nm wavelength by step 6., and calculates 665nm fluorescent emission intensity and 620nm fluorescent emission intensity ratio obtains liquid fluorescence ratio to be measured;
Step 7. is by liquid fluorescence ratio to be measured and standard fluorescence curve comparison thus obtain the concentration of heavy metal ion in aqueous solution to be detected, and described standard fluorescence curve is the fluorescence curve that the liquid fluorescence ratio obtained based on standard solution and the described fluoroscopic examination liquid of several known heavy metal concentration calibrates.
Above-mentioned heavy metal comprises Hg
2+, Cu
2+, Pb
2+; The stir speed (S.S.) of above-mentioned steps 1 ~ 4 is 300rpm.
The optical source wavelength that in above-mentioned steps 6, excitation source produces is 360nm.In above-mentioned steps 2, step 4, the time of coupling reaction is 2h.
In above-mentioned steps 2, step 4, the temperature of coupling reaction is 25 DEG C.
Above content is further described below.
Reaction principle as shown in Figure 1, Eu3+ salt modify papain amounts of components, concentration is as shown in table 1.
Table 1
| Reagent | Concentration | Molar ratio |
| Eu3+ salt | 0.68uM | 1(final concentration is 10nM) |
| Papain | 1 uM | 5 |
| EDC | 1 uM is dissolved in PBS(pH7.4) | 20~50 |
| N-hydroxy thiosuccinimide | 1 uM is dissolved in PBS(pH7.4) uM, i.e. umol/L | 20~50 |
The papain amounts of components that fluorescent dye XL665 modifies, concentration are as shown in table 2:
Table 2
| Reagent | Concentration | Molar ratio |
| XL665 | 0.1uM | 1(final concentration is 10nM) |
| Papain | 1uM | 5 |
| EDC | 1 uM | 20~50 |
| N-hydroxy thiosuccinimide | 1 uM | 20~50 |
The present embodiment standard curve is drawn as follows: by Eu
3+the heavy metal ion of variable concentrations is added in the papain mixed solution that salt modifying pawpaw enzyme and fluorescent dye XL665 modify, be respectively 0.1 nM, 1 nM, 10 nM, 100 nM, 1 nM, by the excitation light source excites of 360nm, determined wavelength in the fluorescent emission intensity of 620nm and 665nm, and calculates the ratio of 665nm/620nm fluorescence intensity.According to ratio drawing standard curve.
(1) by Eu
3+salt (final concentration is 10nM) joins in reactor; Add 300nM EDC solution and 300nM sulfo-NHS solution, activation 30min; Add 50nM papain, continue concussion and mix, 300rpm, 25 DEG C, 2h, each reactant molar concentration rate is: Eu
3+salt: papain: EDC:sulfo-NHS=1:5:30:30, sample bag filter purifying removes unnecessary papain and activator 48h, thus removes unnecessary papain and activator, and the papain obtaining purifying modifies Eu3+ salt.
(2) be 10nM by fluorescent dye XL665(final concentration) join in reactor; Add 300nM EDC solution and 300nM sulfo-NHS solution, activation 30min; Add 50nM papain, continue concussion to mix, 300rpm, 25 DEG C, 2h, each reactant molar concentration rate is: XL665: papain: EDC:sulfo-NHS=1:5:30:30, and this ratio fully can activate XL665 surface amino groups group, can improve coupling efficiency, too can not waste reagent again, sample bag filter purifying removes unnecessary papain and activator 48h, thus removes unnecessary papain and activator, and the papain obtaining purifying modifies XL665.
(3) Eu above-mentioned steps obtained
3+salt modifying pawpaw enzyme and XL665 modifying pawpaw enzyme fully mix, detect fluorescent assay signal, as shown in Figure 2, in Fig. 2, y=0.318x+4.268 is typical curve computing formula, 665nm/620nm ratio step 5 in example 1 obtained is brought into and is substituted in this formula as y value, calculate the concentration that x value is heavy metal substance in testing sample, the linearly dependent coefficient of R2 formula is for this reason the statistical study index of correlationship level of intimate between x, y;
(4) in above-mentioned mixed solution, add the heavy metal ion of variable concentrations respectively, as 0.1 nM, 1 nM, 10 nM, 100 nM, 1 uM, by the excitation light source excites of 360nm, determined wavelength in the fluorescent emission intensity of 620nm and 665nm, and calculates the ratio of 665nm/620nm fluorescence intensity.According to ratio drawing standard curve as reference, to calculate the middle concentration of heavy metal ion of testing sample, typical curve is contrast effect, and the ratio of the 665nm/620nm obtained by test sample is brought in typical curve equation, by can be calculated the concentration of heavy metal in waste water material to be measured.
(5) waste water material to be measured is added in the mixed solution that above-mentioned (3) obtain; By the excitation light source excites of 360nm, determined wavelength is in the fluorescent emission intensity of 620nm and 665nm; Calculate the ratio of 665nm/620nm fluorescence intensity, and the typical curve reference obtained with above-mentioned (4), the ratio being about to the 665nm/620nm that test sample obtains is brought in typical curve equation, by can be calculated the concentration of heavy metal in waste water material to be measured, the ratio of the 665nm/620nm fluorescence intensity obtained is brought in typical curve, thus calculates the concentration of heavy metal ions in wastewater to be measured.Utilize the metal ion detection obtained of this method to be limited to: 0.1nM, can obtain following result, be illustrated in figure 3 control test, be do not having heavy metal ion to deposit in case, the fluorescence spectrum figure obtained, does not namely have XL665 characteristic peak, only has EU
3+the fluorescence spectrum figure experimental data of salt characteristic peak.If Fig. 4 is wastewater sample testing experiment, deposit in case in heavy metal ion, the fluorescence spectrum figure obtained, i.e. XL665 characteristic peak and EU
3+the simultaneous fluorescence spectrum figure of salt characteristic peak, in figure, red characteristic peak is the characteristic peak of XL665.
Above-described embodiment, only for technical conceive of the present invention and feature are described, its object is to person skilled in the art can be understood content of the present invention and implement according to this, can not limit the scope of the invention with this.All equivalences done according to Spirit Essence of the present invention change or modify, and all should be encompassed within protection scope of the present invention.
Claims (7)
1. a detection method for high sensitivity heavy metal in water content, is characterized in that: comprise the following steps:
Step 1. will containing Eu
3+the N-hydroxy thiosuccinimide solution of the europium cryptate of ion and the EDC solution of 300nM and 300nM is uniformly mixed and activates 30min and form first and activate mixed liquor;
50nM papain and described first are activated mixed liquor and are uniformly mixed coupling reaction under the condition of 20 DEG C ~ 30 DEG C and form Eu by step 2.
3+the papain that salt is modified, described containing Eu
3+the europium cryptate of ion and the molar ratio of papain are 1:5, described containing Eu
3+the molar ratio of the europium cryptate of ion and EDC and N-hydroxy thiosuccinimide is 1:2 ~ 5, described Eu
3+the papain that salt is modified filters the Eu after obtaining purifying through bag filter
3+the papain that salt is modified;
The N-hydroxy thiosuccinimide solution of the EDC solution of fluorescent dye XL665 and 300nM and 300nM is uniformly mixed activation 30min formation second activation mixed liquor by step 3.;
50nM papain and described second are activated mixed liquor and are uniformly mixed coupling reaction under the condition of 20 DEG C ~ 30 DEG C and form the papain that fluorescent dye XL665 modifies by step 4., in described fluorescent dye XL665 and step 4, the molar ratio of papain is 1:5, and the molar ratio of described fluorescent dye XL665 and EDC and N-hydroxy thiosuccinimide is 1:2 ~ 5;
Step 5. is by described Eu
3+the papain that salt is modified and the papain that fluorescent dye XL665 modifies are thoroughly mixed to form fluoroscopic examination liquid, described Eu
3+the papain that salt is modified fully mixes according to 1:1 mol ratio with the papain that fluorescent dye XL665 modifies;
Described fluoroscopic examination liquid is added the excitation light source excites of warp after aqueous solution to be detected thus the fluorescent emission intensity detected at 620nm and 665nm wavelength by step 6., and calculates 665nm fluorescent emission intensity and 620nm fluorescent emission intensity ratio obtains liquid fluorescence ratio to be measured;
Step 7. is by liquid fluorescence ratio to be measured and standard fluorescence curve comparison thus obtain the concentration of heavy metal ion in aqueous solution to be detected, and described standard fluorescence curve is the fluorescence curve that the liquid fluorescence ratio obtained based on standard solution and the described fluoroscopic examination liquid of several known heavy metal concentration calibrates.
2. method according to claim 1, is characterized in that: the papain that XL665 described in step 4 modifies filters the papain of the modification of the XL665 after obtaining purifying through bag filter.
3. method according to claim 1, is characterized in that: described heavy metal comprises Hg
2+, Cu
2+, Pb
2+.
4. method according to claim 1, is characterized in that: the stir speed (S.S.) of described step 1 ~ 4 is 300rpm.
5. method according to claim 1, is characterized in that: the optical source wavelength that in described step 6, excitation source produces is 360nm.
6. method according to claim 1, is characterized in that: in described step 2, step 4, the time of coupling reaction is 2h.
7. method according to claim 1, is characterized in that: in described step 2, step 4, the temperature of coupling reaction is 25 DEG C.
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| CN110354826A (en) * | 2019-07-29 | 2019-10-22 | 肇庆学院 | A kind of lead cadmium ion double-template magnetic molecularly imprinted polymer and preparation method thereof |
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| US6689574B1 (en) * | 1997-10-07 | 2004-02-10 | Merck & Co., Inc. | Assays for nuclear receptor agonists and antagonists using fluorescence resonance energy transfer |
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| CN102007395A (en) * | 2008-04-17 | 2011-04-06 | 凯杰博登湖有限公司 | Fluorescence standard, and the use thereof |
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| US7994274B2 (en) * | 2003-09-02 | 2011-08-09 | I.S.T. (Ma) Corporation | Two-stage cure polyimide oligomers |
| DE602007010459D1 (en) * | 2007-09-04 | 2010-12-23 | Inst Nat Sante Rech Med | Use of compounds that prevent EF-Tu-Nucleolin interaction for the prevention or treatment of tularemia |
| US20120202702A1 (en) * | 2011-02-07 | 2012-08-09 | The Regents Of The University Of California | Detection of low concentration biological agents |
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| US6689574B1 (en) * | 1997-10-07 | 2004-02-10 | Merck & Co., Inc. | Assays for nuclear receptor agonists and antagonists using fluorescence resonance energy transfer |
| WO2006044747A2 (en) * | 2004-10-15 | 2006-04-27 | Signal Pharmaceuticals, Llc | P27 ubiquitination assay and methods of use |
| CN102007395A (en) * | 2008-04-17 | 2011-04-06 | 凯杰博登湖有限公司 | Fluorescence standard, and the use thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110354826A (en) * | 2019-07-29 | 2019-10-22 | 肇庆学院 | A kind of lead cadmium ion double-template magnetic molecularly imprinted polymer and preparation method thereof |
| CN110354826B (en) * | 2019-07-29 | 2021-12-14 | 肇庆学院 | A kind of lead-cadmium ion double-template magnetic molecularly imprinted polymer and preparation method thereof |
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| CN103196877A (en) | 2013-07-10 |
| CN103196877B (en) | 2015-06-03 |
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Effective date of registration: 20190617 Address after: 215000 Chengyang International Science and Technology Pioneer Park, Xiangcheng Economic Development Zone, Suzhou, Jiangsu Province Patentee after: Ord Shinco (Suzhou) Medical Technology Co Ltd Address before: 215513 Jiangsu science and Technology Development Zone, Suzhou four Changshu Road 11 Chuang Chuang 201 Co-patentee before: Yang Xiaodong Patentee before: Liu Xiaolong Co-patentee before: Zhang Yun |