CN104786277B - Chondroconia acquisition device - Google Patents

Abstract

The invention discloses a device for obtaining cartilage particles, which belongs to the technical field of medical devices, and comprises a grinding drill bit for cutting cartilage particles, a collection system for collecting cartilage particles, a motor for driving the drill bit to rotate, and a negative pressure protective cover set on the grinding drill bit. The front end of the grinding drill bit is a spherical or conical cutting head, and the rear end is a grinding drill rod. The surface of the cutting head is provided with wedge-shaped serrated blades in a spiral arrangement. The inner surface of the negative pressure protective sleeve and the outer surface of the grinding drill rod The surface and the closed end of the negative pressure protective sleeve form a ring-shaped particle channel, and the collection system includes a negative pressure device arranged outside the negative pressure protective cover that can generate adsorption force on the particle channel and a device for collecting cartilage particles that communicates with the particle channel. collector. The device can complete the preparation and collection of cartilage particles at one time, saves time and improves efficiency, and ensures that the size of cartilage particles is between 100-150 μm, and the chondrocytes in the particles have a high survival rate and strong division and proliferation ability.

Description

Cartilage particle harvesting device

technical field

The invention belongs to the technical field of medical devices, and in particular relates to a device for obtaining cartilage particles.

Background technique

Articular cartilage defects are common in clinical practice. According to statistics, more than 60% of knee arthroscopic surgery patients are accompanied by cartilage defects, of which 42% are focal hyaline cartilage defects, that is, the depth of the defect does not exceed the calcified cartilage layer, and the defect area is ^2-4cm2 between. Since hyaline cartilage has no blood vessels, nerves, and lymphatic system, its natural repair ability is low, and defects with a diameter of more than 4mm can hardly be completely repaired. If left untreated, the damage can progress and lead to arthritis, painful, swollen, deformed joints. For this kind of cartilage defect, the traditional treatment strategy is to use microfracture method to exude blood from the bone marrow, and use the mesenchymal stem cells in the blood clot to proliferate and repair the cartilage defect, but the fibers with poor wear resistance are finally formed cartilage. With the rapid development of tissue engineering technology, autologous chondrocyte transplantation technology is gradually being applied clinically, but this technology requires in vitro expansion of autologous chondrocytes, and then a second surgical implantation; because in vitro culture is difficult to simulate the in vivo microenvironment, In particular, due to the lack of monitoring by the body's immune system, the implanted chondrocytes have usually undergone phenotypic changes, so the final repair tissue is fundamentally different from natural cartilage in terms of structure and composition, making it difficult to ensure the long-term effectiveness of its treatment .

In view of the above problems, we proposed: using autologous cartilage microparticles transplantation strategy to transfer the in vitro expansion process of chondrocytes to the body to avoid their dedifferentiation; at the same time, supplemented with cartilage biomimetic matrix sol for dispersion and fixation, and using the in vivo microenvironment to realize chondrocyte proliferation , secrete matrix, and regenerate high-quality articular cartilage. Previous studies have shown that the average density of articular chondrocytes is 14,000/mm ³ , and the cartilage can be crushed mechanically to prepare micron-sized cartilage particles, so that each particle contains chondrocytes with proliferation ability, and then compounded with cartilage biomimetic matrix sol implanted in vivo. The chondrocytes in the cartilage particles use the in vivo microenvironment to simulate the natural cartilage formation process to proliferate, secrete matrix, and regenerate and repair cartilage defects.

Compared with the traditional autologous chondrocyte transplantation technology, this strategy has the following advantages: the chondrocytes in the cartilage are dispersed in the form of cartilage particles by mechanical pulverization, without the need for separation and expansion of chondrocytes, and the bionic matrix sol is used in one operation It can be completed; the in vivo microenvironment is used to realize the proliferation of chondrocytes, avoiding the dedifferentiation of chondrocytes caused by in vitro expansion, thereby improving the quality of regenerated cartilage; obtaining a small amount of autologous cartilage from the edge of cartilage defects, waste utilization can minimize trauma, and is not affected. Cartilage defect shape restriction. After the implementation of this project, it is expected to realize the timely repair of focal articular cartilage defects found during the operation, stop the pathological process of arthritis caused by them, relieve pain of patients, and restore joint movement function, which has a wide application prospect.

However, to achieve the above goals, it is first necessary to solve the technical problem of autologous cartilage microparticles preparation. Previous studies have shown that chondrocytes have the strongest proliferation ability in particles with a particle size of 100-150 μm, but how to efficiently obtain cartilage particles in this particle size range? And minimize the mechanical damage of chondrocytes during the preparation process? It is a key technical problem that needs to be solved urgently to realize the above-mentioned goals. In the previous study, we tried to prepare cartilage microparticles by manual cutting. Although the proliferation activity of chondrocytes in the microparticles could be guaranteed, there were problems such as difficulty in controlling the particle size, high labor intensity, and low efficiency. The diameter is controllable, the particles are uniform, and the efficiency is high. However, due to the heat generated by the grinding wheel during the grinding process, the chondrocytes in the particles lose their activity, and the proliferation of chondrocytes cannot be achieved.

Therefore, it is necessary to develop a device for obtaining autologous cartilage particles, which can efficiently obtain cartilage particles with a particle size of 100-150 μm, while minimizing the mechanical damage of chondrocytes during the preparation process, and ensuring the proliferation activity of chondrocytes in the cartilage particles.

Contents of the invention

In view of this, the purpose of the patent of the present invention is to develop a device for obtaining autologous cartilage particles to solve the technical problems of autologous cartilage particles. Using this device can efficiently obtain cartilage particles with a particle size of 100-150 μm, while minimizing the amount of cartilage in the preparation process. The mechanical damage of the cells ensures the proliferative activity of the chondrocytes in the cartilage microparticles. At the same time, it solves the problems in the prior art that the particle size is difficult to control, the labor intensity is high, and the proliferative activity of chondrocytes is low.

The purpose of the present invention is achieved through the following technical solutions:

The device for obtaining cartilage particles of the present invention includes a drill bit for cutting cartilage particles, a collection system for collecting cartilage particles, a motor for driving the drill bit to rotate at high speed, and a negative pressure protective sleeve set on the drill bit, the front end of the drill bit is spherical Or a tapered cutting head, the rear end of which is a grinding drill pipe, the surface of the cutting head is provided with wedge-shaped serrated blades arranged in a spiral manner, the negative pressure protective sleeve is a tubular structure with one end open and the other end closed, and the closed end is provided with There is a drill rod through hole that can be sealed by the safety protective sleeve and is set on the drill rod, and its open end forms an annular particle inlet with the cutting head of the drill bit. The outer surface and the closed end of the negative pressure protective sleeve form a ring-shaped particle channel. The far end of the drill rod extends out of the negative pressure protective sleeve and is fixed with the drive shaft of the motor. A vacuum pump that generates adsorption force on the particle channel and a collector connected to the particle channel for collecting cartilage particles, the collection system also includes a water spray device that can dilute the particles, and the water spray device includes The water spray pump communicated with the particle channel outside the casing.

Further, a plurality of particle holes are provided between the wedge-shaped sawtooth blades and between the serrations, and the drill rod of the drill bit is a hollow tube, the front end of the hollow tube communicates with the particle holes, and the rear end of the hollow tube communicates with the particle holes. Seal with a plug.

Further, the hollow tube is provided with a plurality of distribution holes evenly distributed on its circumference on the two cross-sections where the inlets of the vacuum pump and the water jet pump enter the negative pressure protective sleeve, respectively, to communicate with the particle channel.

Further, the end of the safety sheath is provided with a protective cover conforming to the cutting head of the drilling and grinding head.

Further, the collector is a collection box, and a box seat for movably connecting the collection box is arranged between the collection box and the negative pressure protective sleeve, and the movably connected is a protrusion arranged on the outer wall of the mouth of the collection box and arranged on the There are at least two grooves on the box base that closely match the protrusions, and there are at least two movable connections, which are evenly distributed around the collection box and the box base.

Further, the diameter of the spherical or conical cutting head of the drill bit is 2-5mm.

Further, the wedge-shaped sawtooth blades are 100-150 μm high, and the distance between two adjacent blades is 100-150 μm.

Further, the pore size of the particle pores is 100-150 μm.

Further, the diameter of the hollow tube is 150-800 μm.

Further, it also includes a casing arranged outside the motor, and a control switch button is also arranged on the casing.

The beneficial effects of the present invention are:

1. By setting the grinding drill bit and the negative pressure protective sleeve, and setting the grinding drill bit in the negative pressure protective sleeve to form a particle channel that can generate negative pressure to collect particles, it is possible to collect cartilage particles while producing cartilage particles , Complete the process of cutting particles and collecting particles at one time, which greatly saves the preparation time and improves the production efficiency. At the same time, because the negative pressure protective sleeve can protect the operation process, the process of preparing particles is safer.

2. The depth of the blade and the distance between the blades are set to 100-150 μm, which can ensure that the particle size is uniform and within 100-150 μm, so that the chondrocytes in the particles have a high survival rate and a strong ability to divide and proliferate.

3. By setting particle holes with a diameter of 100-150 μm and communicating with the particle channel through the hollow tube, the particle channel is connected with the vacuum pump and the water spray pump. On the one hand, during the preparation process, the cartilage particles can be sprayed with water through the hollow tube Dilution, on the other hand, during the collection process, the diluted particles can be collected through a hollow tube, and the size of the cartilage particles can be controlled within the range of 100-150 μm, so as to avoid the influence of excessive particles on the proliferation ability, that is, another channel is added Preparation and collection of cartilage microparticles can prevent the microparticles from clogging on the cutting head and affecting the production efficiency.

4. Since the present invention is a mechanized operation and adopts dual-channel operation, it solves the problems in the prior art of high labor intensity for preparation, large demand for autologous cartilage sources, and easy loss of activity of the prepared particles. It has simple structure, It has the advantages of convenient operation and high production efficiency, and the size of the produced cartilage particles is only 100-150 μm, with strong proliferation ability and less demand for autologous cartilage sources, which relieves the pain of patients in the production of cartilage, and also greatly slows down the process of cartilage production. The patient's treatment cycle provides a prerequisite guarantee for the realization of the concept of "tissue engineered cartilage bionic construction".

Description of drawings

In order to make the purpose, technical scheme and beneficial effect of the present invention clearer, the present invention provides the following drawings for illustration:

Fig. 1 is a schematic diagram of the structure of the device for obtaining cartilage microparticles of the present invention.

Reference signs: 1-grinding drill bit; 2-negative pressure protective sleeve; 3-cutting head; 4-grinding drill pipe; 5-motor; 6-splitting hole; 7-hollow tube; 8-box seat; 9-collect Box; 10-vacuum pump; 11-protrusion; 12-groove; 13-shell; 14-control switch button; 15-particle channel; 16-wedge serrated blade; 17-particle hole; Circle; 20-protective cover; 21-sawtooth.

detailed description

The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

As shown in Figure 1, it includes a grinding drill bit 1 for cutting cartilage particles, a collection system for collecting cartilage particles, a motor 5 that drives the grinding drill bit 1 to rotate at a high speed, and a negative pressure protective cover 2 that is set on the grinding drill bit 1. The front end of the drill bit 1 is a spherical or conical cutting head 3, and the rear end is a grinding drill rod 4. The surface of the cutting head 3 is provided with a wedge-shaped serrated blade 16 arranged in a spiral, that is, the thickness of the wedge-shaped serrated blade 16 is determined by the cutting head 3 and the The joint part of the grinding drill rod 4 to the front end of the wedge-shaped sawtooth blade 16 gradually decreases to form a cutting part to cut the cartilage, and the sawtooth 21 also has a cutting effect on the cartilage, so that the cartilage is further reduced. The negative pressure protective sleeve 2 is The tubular structure is open at one end and closed at the other end. The closed end is provided with a drill rod through hole that can seal the safety protective sleeve and fit on the drill rod. The open end and the cutting head of the drill bit form a ring-shaped particle inlet. The inner surface of the negative pressure protective cover, the outer surface of the grinding drill rod and the closed end of the negative pressure protective cover form an annular particle channel 15, and the far end of the grinding drill rod 4 extends out of the negative pressure protective cover 2 and the motor. The drive shaft is fixed, and due to low-speed friction and heat generation, the damage is large. The motor 5 in this embodiment is controlled at a speed of 4000-15000r/min, and the grinding head is driven to cut at high speed instantaneously, with little damage to cartilage particles and better cell activity. , the collection system includes a negative pressure device arranged outside the negative pressure protective sheath that can generate adsorption force on the particle channel and a collector for collecting cartilage particles communicated with the particle channel, and the negative pressure device is a vacuum pump 10 .

In this embodiment, the grinding drill bit 1 and the negative pressure protective cover 2 are arranged, and the grinding drill bit 1 is arranged in the negative pressure protective cover 2, and the inner surface of the negative pressure protective cover 2, the outer surface of the grinding drill rod 4 and the Negative pressure is generated in the ring-shaped particle channel 15 formed by the closed end of the negative pressure protective sleeve 2 to collect particles. Cartilage particles can also be collected while producing cartilage particles, and the process of cutting particles and collecting particles can be completed at one time. It greatly saves the preparation time and improves the production efficiency. At the same time, because the negative pressure protective sleeve can protect the operation process, the process of preparing particles is safer.

As an improvement of this embodiment, the collection system further includes a water spraying device capable of diluting the particles, and the water spraying device includes a water spray pump 18 arranged outside the negative pressure protective sheath 2 and communicated with the particle channel 15 . During the preparation process, the water spray pump 18 can spray and dilute the cartilage particles through the hollow tube 7 to change the cartilage particles into a cartilage solution.

As an improvement of this embodiment, a plurality of particle holes 17 are provided between the wedge-shaped sawtooth blades 16 and between the serrations 21, the drill rod of the drill bit is a hollow tube 7, and the front end of the hollow tube 7 It communicates with the particle hole 17, and its rear end is sealed with a plug. The hollow pipe 7 is respectively provided with a plurality of The shunt holes 6 evenly distributed on its circumference communicate with the microparticle channel 15, through the shunt holes 6, the vacuum pump 10 and the hollow tube 7 can be more easily connected to form a state of communication, which is easy to generate negative pressure, thereby realizing the collection of cartilage particles. A positive pressure can be formed between the water jet pump 18 and the hollow tube 7 through the split hole 6, so as to realize the dilution of the cartilage particles.

By setting the particle hole 17 with an aperture of 100-150 μm and communicating with the particle channel 15 through the hollow tube 7, the particle channel 15 is connected with the vacuum pump 10 and the water jet pump 18. On the one hand, during the preparation process, the hollow tube 7 can Spray water to dilute the cartilage particles. On the other hand, during the collection process, the diluted particles can be collected through the hollow tube 7, and the size of the cartilage particles can be controlled within the range of 100-150 μm, so as to avoid the influence of excessive particles on the proliferation ability , on the basis of the original particle channel 15, another channel is newly added, that is, a hollow tube channel for preparing and collecting cartilage particles, which can avoid the problem that the particles are blocked on the cutting head and affect the production efficiency. At the same time, it can also use This channel cleans and unblocks the particles concentrated on the cutting head from the inside of the cutting head, making the preparation smoother and more efficient.

As an improvement of this embodiment, the end of the negative pressure protective sleeve 2 is provided with a protective cover 20 conforming to the cutting head 3 of the drilling and grinding head. It can cut cartilage normally, and on the other hand, it can prevent particles from splashing and reduce waste.

As an improvement of this embodiment, the collector is a collection box 9, and a box seat 8 for movably connecting the collection box is arranged between the collection box 9 and the negative pressure protective cover 2, and the movably connected is arranged on the collection box 9 protrusions 11 on the outer wall of the mouth and grooves 12 that are arranged on the box seat and closely match the protrusions. There are at least two flexible connections, and they are evenly distributed around the collection box 9 and the box seat 8. By setting the box seat 8, the particle collection box 9 is fixed reliably, and it is easy to disassemble. At the same time, a convex-concave structure is provided to facilitate connection and processing, which can improve installation efficiency.

As an improvement of this embodiment, it also includes a casing 13 arranged outside the motor 5 , and the outer surface of the casing is provided with textures, which is convenient for hands to hold during the production process.

As an improvement of this embodiment, the control switch button 14 of the motor 5, the water jet pump 18 and the vacuum pump 10 is arranged on the housing, which facilitates the control of the device, makes the preparation process accurate, and avoids misoperation

As an improvement of this embodiment, the diameter of the spherical or conical cutting head of the drill bit is 2-5mm.

As an improvement of this embodiment, the wedge-shaped serrated blades 16 are 100-150 μm high, and the distance between two adjacent blades is 100-150 μm. The particles produced by grinding can be controlled within the range of 100-150μm.

As an improvement of this embodiment, the diameter of the particle holes 17 is 100-150 μm. The particles passing through the particle hole 17 can be controlled within the range of 100-150 μm.

As an improvement of this embodiment, the diameter of the hollow tube 7 is 150-800 μm. Particles with a particle size of 100-150 μm can pass through smoothly, and it is not easy to clog due to adhesion.

Finally, it should be noted that the above preferred embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail through the above preferred embodiments, those skilled in the art should understand that it can be described in terms of form and Various changes may be made in the details without departing from the scope of the invention defined by the claims.

Claims (10)

1. chondroconia acquisition device, is characterized in that: comprise crusher drill for cutting chondroconia, collect softThe gathering system of bone particulate, drive the motor of crusher drill High Rotation Speed and be sleeved on the Negative pressure prevention on crusher drillCover, described crusher drill front end is spherical or taper cutting head, and rear end is abrasive drilling bar, and establish on described cutting head surfaceBe equipped with and be spiral-distributed wedge shape serrated knife blade, described Negative pressure prevention cover is that an end opening, the other end sealTubular structure, its blind end is provided with can be by the abrasive drilling bar mistake on abrasive drilling bar that is sleeved on of Negative pressure prevention cover sealingHole, the cutting head of its openend and crusher drill forms ring-type particulate entrance, the inner surface of described Negative pressure prevention cover,The blind end of the outer surface of abrasive drilling bar and Negative pressure prevention cover forms ring-type passage of particles, the far-end of described abrasive drilling barExtend Negative pressure prevention cover and fix with the driving shaft of motor, described gathering system comprises and is arranged on Negative pressure prevention coverOutward can to passage of particles produce the vavuum pump of absorption affinity and be communicated with passage of particles for collecting chondroconiaCollector, described gathering system also comprises the water injector that can dilute particulate, described water injector bagDraw together and be arranged on the outer water jet pump being communicated with passage of particles of Negative pressure prevention cover.

2. chondroconia acquisition device according to claim 1, is characterized in that: described wedge shape serrated knife blade itBetween and between sawtooth, be provided with multiple microparticle pores, the abrasive drilling bar of described crusher drill is hollow tube, described hollow tubeFront end be communicated with described microparticle pores, its rear end seals with plug.

3. chondroconia acquisition device according to claim 2, is characterized in that: described hollow tube is respectively veryEmpty pump and water jet pump enter to be provided with on two cross sections at entrance place of Negative pressure prevention cover and are multiplely distributed on itTap hole on circumference is communicated with passage of particles.

4. chondroconia acquisition device according to claim 1, is characterized in that: the end of described Negative pressure prevention coverPortion is provided with the protective cover conformal with boring bistrique cutting head.

5. chondroconia acquisition device according to claim 1, is characterized in that: described collector is disposable box,Between described disposable box and Negative pressure prevention cover, be provided with the cassette holder for being flexibly connected disposable box, described movable companyConnect the projection for being arranged on disposable box oral area outer wall and be arranged on cassette holder and the groove of the mutual close-fitting of projection instituteState flexible connection and be at least two, and be distributed on the surrounding of disposable box and cassette holder.

6. chondroconia acquisition device according to claim 1, is characterized in that: described crusher drill spherical orThe diameter of conical cutting head is 2-5mm.

7. chondroconia acquisition device according to claim 1, is characterized in that: described wedge shape serrated knife blade is high100-150 μ m, the spacing between two adjacent edge is 100-150 μ m.

8. chondroconia acquisition device according to claim 1, is characterized in that: the aperture of described microparticle pores is100-150μm。

9. chondroconia acquisition device according to claim 2, is characterized in that: the caliber of described hollow tube is150-800μm。

10. according to the arbitrary described chondroconia acquisition device of claim 1-9, it is characterized in that: also comprise settingAt the shell of outside motor, on described shell, be also provided with gauge tap button.