CN104758315B - Method for coating fish oil by using zein - Google Patents
Method for coating fish oil by using zein Download PDFInfo
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- CN104758315B CN104758315B CN201410005004.9A CN201410005004A CN104758315B CN 104758315 B CN104758315 B CN 104758315B CN 201410005004 A CN201410005004 A CN 201410005004A CN 104758315 B CN104758315 B CN 104758315B
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- 235000021323 fish oil Nutrition 0.000 title claims abstract description 199
- 229920002494 Zein Polymers 0.000 title claims abstract description 162
- 239000005019 zein Substances 0.000 title claims abstract description 62
- 229940093612 zein Drugs 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 28
- 239000011248 coating agent Substances 0.000 title abstract 2
- 238000000576 coating method Methods 0.000 title abstract 2
- 238000001035 drying Methods 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract 2
- 239000000243 solution Substances 0.000 claims description 196
- 239000000843 powder Substances 0.000 claims description 74
- 235000019198 oils Nutrition 0.000 claims description 53
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 48
- 239000011259 mixed solution Substances 0.000 claims description 45
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 42
- 239000003208 petroleum Substances 0.000 claims description 24
- 239000011258 core-shell material Substances 0.000 claims description 4
- 239000004005 microsphere Substances 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 abstract description 48
- 238000002360 preparation method Methods 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 4
- 238000001704 evaporation Methods 0.000 abstract description 3
- 238000001338 self-assembly Methods 0.000 abstract description 3
- 238000004090 dissolution Methods 0.000 abstract description 2
- 239000003814 drug Substances 0.000 abstract description 2
- 230000008020 evaporation Effects 0.000 abstract description 2
- 235000013305 food Nutrition 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000009467 reduction Effects 0.000 abstract description 2
- 108010055615 Zein Proteins 0.000 description 146
- 239000003921 oil Substances 0.000 description 51
- 238000001157 Fourier transform infrared spectrum Methods 0.000 description 29
- 235000019441 ethanol Nutrition 0.000 description 26
- 239000011888 foil Substances 0.000 description 20
- 230000000873 masking effect Effects 0.000 description 20
- 238000013019 agitation Methods 0.000 description 18
- 240000008042 Zea mays Species 0.000 description 15
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 15
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 15
- 235000005822 corn Nutrition 0.000 description 15
- 238000005033 Fourier transform infrared spectroscopy Methods 0.000 description 8
- 238000012790 confirmation Methods 0.000 description 8
- 239000000463 material Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 238000002604 ultrasonography Methods 0.000 description 4
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 3
- 238000005538 encapsulation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 229920001661 Chitosan Polymers 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010020466 Hunger Diseases 0.000 description 1
- 102000004407 Lactalbumin Human genes 0.000 description 1
- 108090000942 Lactalbumin Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000003917 TEM image Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 230000004641 brain development Effects 0.000 description 1
- 230000003925 brain function Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000011203 carbon fibre reinforced carbon Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
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- 230000000694 effects Effects 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- JAZBEHYOTPTENJ-UHFFFAOYSA-N eicosapentaenoic acid Natural products CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O JAZBEHYOTPTENJ-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
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- 239000003292 glue Substances 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
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- 238000005286 illumination Methods 0.000 description 1
- 235000021388 linseed oil Nutrition 0.000 description 1
- 239000000944 linseed oil Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
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- 239000012460 protein solution Substances 0.000 description 1
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- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Landscapes
- General Preparation And Processing Of Foods (AREA)
Abstract
The invention provides a method for coating fish oil by using zein, which comprises the following steps: s1, dissolving zein in an ethanol water solution, and stirring until the solution is uniform and transparent to prepare a zein solution; s2, dissolving the fish oil in the ethanol water solution, and stirring until the solution is uniform and transparent to prepare a fish oil solution; s3, mixing the zein solution and the fish oil solution; and S4, drying. The fish oil is wrapped by the zein evaporation self-assembly, so that the fish oil can be better protected, the reduction of the nutrition and medicinal value of the fish oil is avoided, the taste of the fish oil is eliminated, the dissolution property of the fish oil is changed, meanwhile, complex equipment is not needed in the wrapping process of the fish oil, the preparation method is simple, and the obtained product can be widely applied to the fields of food, medicine and the like.
Description
Technical field
The invention belongs to both sexes natural macromolecular material technical fields, more specifically, being related to a kind of molten using corn alcohol
The method of protein encapsulation fish oil.
Background technique
Eicosapentaenoic acid abundant (EPA) and 22 carbon, six diluted acid (DHA), these ω -3 insatiable hungers are rich in fish oil
It is the substance with different physiological roles important in fish oil, nutritive value with higher even medical value with fatty acid.
It, can be with studies have shown that EPA and DHA has active influence to the maintenance of the brain development and function of teenager and adult
It is used to treat many cardiovascular diseases, also played an important role in the treatment and prevention of cancer.But due to these
In polyunsaturated fatty acid contain more double bond, illumination and it is aerobic under conditions of be easily oxidized it is rotten, occur acid
It loses, leads to the missing of nutritive value and medical value；In addition, the taste of fish oil can give a kind of offending feeling of people.For
Both reasons, many different measures have been used for the protection of fish oil, including the microencapsulation of fish oil.In microcapsules
In change technology, the core material of different conditions is wrapped up by natural or synthetic high molecular material, and can be released under certain condition
It releases.Studies have shown that fish oil can wrap up protection by many different materials.These lappings can be single
Be also possible to be combined by different materials and be formed, including lactalbumin, skimmed milk power, casein, upgraded fiber element, gelatin-Ah
Draw the gummy coacervate of uncle and chitosan.But successfully these package examples in, used lapping all compared with
For complexity, and the step of packaging method is also more various.
Zeins is the major storage albumen in corn, is the by-product of corn wet processing.Since it contains
50% or more hydrophobic grouping, zeins are a kind of Amphiphilic proteins.This relatively inexpensive albumen serves many purposes,
Such as wrap up linseed oil and essential oil with zeins, other research by zeins and other materials for example
Chitosan is in conjunction with wrapping up vitamin e.But at present there has been no research institution to zeins be applied to fish oil package
It has been reported.
Summary of the invention
Technical problem to be solved by the present invention lies in: a kind of method using zeins package fish oil is provided,
Solve the problems such as existing fish oil packaging method is complicated, and lapping is not single, and preparation cost is high.
In the method for the invention using zeins package fish oil, include the following steps:
S1, zeins is dissolved in ethanol water, is stirred to solution homogeneous transparent, so that corn alcohol be made
Molten protein solution；
S2, fish oil is dissolved in ethanol water, is stirred to solution homogeneous transparent, so that fish oil solution be made；
S3, the zein solution is mixed with the fish oil solution；
S4, the zein solution and the mixed solution of the fish oil solution are dried, obtain using corn
Alcohol soluble protein forms zeins-fish oil powder of core-shell structure with microballoon shape package fish oil.
In the method for the invention using zeins package fish oil, in the zein solution and institute
In the mixed solution for stating fish oil solution, the mass ratio of the fish oil and the zeins is 3~10 ﹕ 10.
In the method for the invention using zeins package fish oil, make in the step S1 and the step S2
Stirring means are ultrasonic agitation.
In the method for the invention using zeins package fish oil, ethyl alcohol used in the step S1 is water-soluble
Liquid is the ethanol water that volume fraction is 60%~90%.
In the method for the invention using zeins package fish oil, ethyl alcohol used in the step S2 is water-soluble
Liquid is the ethanol water that volume fraction is 60%~90%.
In the method for the invention using zeins package fish oil, corn in the zein solution
The content of alcohol soluble protein is 0.3mg/ml~1.0mg/ml.
In the method for the invention using zeins package fish oil, the content of fish oil is in the fish oil solution
0.3mg/ml~1.0mg/ml.
In the method for the invention using zeins package fish oil, drying means used in the step S4
To spontaneously dry.
In the method for the invention using zeins package fish oil, the step S4 further comprises: will do
The dry resulting zeins-fish oil powder is cleaned at least once with petroleum ether.
The invention has the advantages that fish oil is wrapped up by evaporating self assembly with zeins, it can be right
Fish oil is preferably protected, and fish oil nutrition and the reduction of medical value are avoided, and eliminates the taste of fish oil, changes the dissolution of fish oil
Property, meanwhile, to, without using complex device, preparation method is simple, and obtained product can be extensive in the encapsulation process of fish oil
Be applied to food, medicine and other fields.
Detailed description of the invention
To describe the technical solutions in the embodiments of the present invention more clearly, make required in being described below to embodiment
Attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only one embodiment of the present of invention, for
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings other
Attached drawing.
Fig. 1 is the FTIR spectrum figure of pure fish oil and pure zeins；
Fig. 2 is that the mass ratio of fish oil and zeins is 4 ﹕ 10, concentration of alcohol 80%(v/v) when gained corn alcohol
Molten albumen-fish oil powder FTIR spectrum figure；
Fig. 3 is that the mass ratio of fish oil and zeins is 3 ﹕ 10, concentration of alcohol 80%(v/v) when gained corn alcohol
Molten albumen-fish oil powder FTIR spectrum figure；
Fig. 4 is that the mass ratio of fish oil and zeins is 5 ﹕ 10, concentration of alcohol 80%(v/v) when gained corn alcohol
Molten albumen-fish oil powder FTIR spectrum figure；
Fig. 5 is that the mass ratio of fish oil and zeins is 6 ﹕ 10, concentration of alcohol 80%(v/v) when gained corn alcohol
Molten albumen-fish oil powder FTIR spectrum figure；
Fig. 6 is that the mass ratio of fish oil and zeins is 8 ﹕ 10, concentration of alcohol 80%(v/v) when gained corn alcohol
Molten albumen-fish oil powder FTIR spectrum figure；
Fig. 7 is that the mass ratio of fish oil and zeins is 10 ﹕ 10, concentration of alcohol 80%(v/v) when gained corn alcohol
Molten albumen-fish oil powder FTIR spectrum figure；
Fig. 8 is that the mass ratio of fish oil and zeins is 3 ﹕ 10, concentration of alcohol 70%(v/v) when gained corn alcohol
Molten albumen-fish oil powder FTIR spectrum figure；
Fig. 9 is that the mass ratio of fish oil and zeins is 3 ﹕ 10, concentration of alcohol 60%(v/v) when gained corn alcohol
Molten albumen-fish oil powder FTIR spectrum figure.
Specific embodiment
Below in conjunction with attached drawing, technical scheme in the embodiment of the invention is clearly and completely described, it is clear that institute
The embodiment of description is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair
The range of bright protection.
The present invention provides a kind of method using zeins package fish oil, and used zeins is day
Right agricultural product by-product, low in cost, from a wealth of sources, biodegradable and compatible become a kind of excellent micro- glue
Capsule lapping, encapsulation process include the following steps:
The preparation of S1, zein solution: zeins is dissolved in ethanol water, stirring to solution
Homogeneous transparent；
The preparation of S2, fish oil solution: fish oil is dissolved in ethanol water, stirring to solution homogeneous transparent；
S3, the zein solution is mixed with the fish oil solution；
S4, drying.
Zeins in volatile spirit solvent can with the evaporation of alcohol and the change of solution polarity and
Self assembly occurs, realizes the package to fish oil.
Fig. 1 shows fourier conversion infrared spectrogram (FTIR) of pure fish oil and pure zeins, two of them
Characteristic peak 3010cm-1(the stretching vibration for representing cis- carbon-carbon double bond) and 1739cm-1(the stretching vibration for representing C=O bond)
Without occurring in the spectrum of zeins in the spectrum of fish oil, if there is any one feature in the product of preparation
Peak appearance then proves that fish oil is wrapped up successfully by zeins.Feature peak-to-peak value may be compared with pure material light in spectral mixture
There is minute movement in spectrum.Feature peak-to-peak value height is related with the content of the fish oil wrapped up.
The present invention also does transmission electron microscope (TEM) structural characterization of product to gained zeins-fish oil powder, dark
Light sphere is fish oil drop among zeins nucleocapsid sphere, it was demonstrated that fish oil is by zeins with microballoon shape packet
Wrap up in into core-shell structure.
Preferably, in the mixed solution of the zein solution and the fish oil solution, the fish oil and institute
The mass ratio for stating zeins is 3~10 ﹕ 10.
Preferably, stirring described in S1 step and S2 step is ultrasonic agitation, and ultrasonic agitation can preferably be realized molten
Solution, so that zeins and fish oil can be well dispersed in ethanol water.
Preferably, the used ethanol water in S1 step and S2 step is the ethyl alcohol that volume fraction is 60%~90%
Aqueous solution.
Preferably, the content of zeins is 0.3mg/ml~1.0mg/ml in the zein solution.
Preferably, the content of fish oil described in the fish oil solution is 0.3mg/ml~1.0mg/ml.
Preferably, drying used in S4 of the present invention is to spontaneously dry, i.e., zeins obtained above is molten
Liquid and fish oil solution mixed solution are placed in the container of wide-mouth, give free rein to drying at normal temperatures and pressures, by mixed solution
Ethyl alcohol volatilizees effect certainly to realize, is that gained produces it is furthermore preferred that should complete masking foil inside the large container used in advance
Object is from being attached on container inner wall, convenient for the collection of product.
Preferably, the S4 step further comprises: dry gained zeins-fish oil powder is cleaned with petroleum ether
At least once, to go out not by zeins package, be attached to the fish oil on powder surface, when cleaning, only needs petroleum ether
Powder surface slowly is flowed through, the petroleum ether of various boiling ranges may be incorporated for above-mentioned cleaning process.
Embodiment 1
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；12mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.4mg/ml, ultrasonic agitation is big
About 10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time
The mass ratio of fish oil and zeins is 4 ﹕ 10 in gained mixed solution, and gained mixed solution is introduced into preprepared
It completes in the culture dish of masking foil and spontaneously dries.Gained zeins-fish oil powder TEM image confirms fish oil by corn
Alcohol soluble protein is rolled into core-shell structure with microballoon shape.Fig. 2 shows gained zeins-fish oil powder FTIR spectrums
Figure, gained zeins-fish oil powder clean the secondary not wrapped fish oil to remove powder surface with petroleum ether,
The confirmation of FTIR spectrum figure has fish oil to be wrapped up by zeins.
Embodiment 2
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；Weigh 9mg's simultaneously
Fish oil is dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.3mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 3 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Fig. 3 shows gained zeins-fish oil powder FTIR spectrum figure, institute
Zeins-fish oil powder is obtained to be cleaned once with petroleum ether to remove the not wrapped fish oil on powder surface, FTIR light
Spectrogram confirmation has fish oil to be wrapped up by zeins.
Embodiment 3
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；15mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.5mg/ml, ultrasonic agitation is big
About 10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time
The mass ratio of fish oil and zeins is 5 ﹕ 10 in gained mixed solution, and gained mixed solution is introduced into preprepared
It completes in the culture dish of masking foil and spontaneously dries.Fig. 4 shows gained zeins-fish oil powder FTIR spectrum figure,
Gained zeins-fish oil powder cleans the secondary not wrapped fish oil to remove powder surface, FTIR with petroleum ether
Spectrogram confirmation has fish oil to be wrapped up by zeins.
Embodiment 4
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；18mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.6mg/ml, ultrasonic agitation is big
About 10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time
The mass ratio of fish oil and zeins is 6 ﹕ 10 in gained mixed solution, and gained mixed solution is introduced into preprepared
It completes in the culture dish of masking foil and spontaneously dries.Fig. 5 shows gained zeins-fish oil powder FTIR spectrum figure,
Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove the not wrapped fish oil on powder surface, FTIR
Spectrogram confirmation has fish oil to be wrapped up by zeins.
Embodiment 5
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；24mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.8mg/ml, ultrasonic agitation is big
About 10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time
The mass ratio of fish oil and zeins is 8 ﹕ 10 in gained mixed solution, and gained mixed solution is introduced into preprepared
It completes in the culture dish of masking foil and spontaneously dries.Fig. 6 shows gained zeins-fish oil powder FTIR spectrum figure,
Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove the not wrapped fish oil on powder surface, FTIR
Spectrogram confirmation has fish oil to be wrapped up by zeins.
Embodiment 6
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；30mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 1mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 10 ﹕ 10, and gained mixed solution is introduced into preprepared
It completes in the culture dish of masking foil and spontaneously dries.Fig. 7 shows gained zeins-fish oil powder FTIR spectrum figure,
Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove the not wrapped fish oil on powder surface, FTIR
Spectrogram confirmation has fish oil to be wrapped up by zeins.
Embodiment 7
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 70%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；Weigh 9mg's simultaneously
Fish oil is dissolved in the 70%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.3mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 3 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Fig. 8 shows gained zeins-fish oil powder FTIR spectrum figure, institute
Zeins-fish oil powder is obtained to be cleaned once with petroleum ether to remove the not wrapped fish oil on powder surface, FTIR light
Spectrogram confirmation has fish oil to be wrapped up by zeins.
Embodiment 8
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 60%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；Weigh 9mg's simultaneously
Fish oil is dissolved in the 60%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.3mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 3 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Fig. 9 shows gained zeins-fish oil powder FTIR spectrum figure, institute
Zeins-fish oil powder is obtained to be cleaned once with petroleum ether to remove the not wrapped fish oil on powder surface, FTIR light
Spectrogram confirmation has fish oil to be wrapped up by zeins.
Embodiment 9
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 90%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；Weigh 9mg's simultaneously
Fish oil is dissolved in the 90%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.3mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 3 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Gained zeins-fish oil powder is cleaned once with petroleum ether to remove powder
The not wrapped fish oil on last surface, gained zeins-fish oil powder FTIR spectrum figure is similar to Example 8, special
This is omitted, and fish oil can also be wrapped up by zeins.
Embodiment 10
Under normal temperature and pressure, weigh the zeins of 9mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, match
The zein solution of 0.3mg/ml is made, about 10min is stirred by ultrasonic, until solution homogeneous transparent；Weigh 9mg's simultaneously
Fish oil is dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.3mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 10 ﹕ 10, and gained mixed solution is introduced into preprepared
It completes in the culture dish of masking foil and spontaneously dries.Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove
The not wrapped fish oil on powder surface, gained zeins-fish oil powder FTIR spectrum figure is similar to Example 6,
It omits hereby, fish oil can also be wrapped up by zeins.
Embodiment 11
Under normal temperature and pressure, weigh the zeins of 12mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 0.4mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；It weighs simultaneously
The fish oil of 12mg is dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.4mg/ml, ultrasound is stirred
About 10min is mixed, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later,
The mass ratio of fish oil and zeins is 10 ﹕ 10 in gained mixed solution at this time, and gained mixed solution is introduced into prior standard
Completing in the culture dish of masking foil for getting ready spontaneously dries.Gained zeins-fish oil powder is cleaned three times with petroleum ether
To remove the not wrapped fish oil on powder surface, gained zeins-fish oil powder FTIR spectrum figure and embodiment 6
It is similar, it omits hereby, fish oil can also be wrapped up by zeins.
Embodiment 12
Under normal temperature and pressure, weigh the zeins of 15mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 0.5mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；It weighs simultaneously
The fish oil of 15mg is dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.5mg/ml, ultrasound is stirred
About 10min is mixed, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later,
The mass ratio of fish oil and zeins is 10 ﹕ 10 in gained mixed solution at this time, and gained mixed solution is introduced into prior standard
Completing in the culture dish of masking foil for getting ready spontaneously dries.Gained zeins-fish oil powder is cleaned three times with petroleum ether
To remove the not wrapped fish oil on powder surface, gained zeins-fish oil powder FTIR spectrum figure and embodiment 6
It is similar, it omits hereby, fish oil can also be wrapped up by zeins.
Embodiment 13
Under normal temperature and pressure, weigh the zeins of 18mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 0.6mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；It weighs simultaneously
The fish oil of 18mg is dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.6mg/ml, ultrasound is stirred
About 10min is mixed, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later,
The mass ratio of fish oil and zeins is 10 ﹕ 10 in gained mixed solution at this time, and gained mixed solution is introduced into prior standard
Completing in the culture dish of masking foil for getting ready spontaneously dries.Gained zeins-fish oil powder is cleaned three times with petroleum ether
To remove the not wrapped fish oil on powder surface, gained zeins-fish oil powder FTIR spectrum figure and embodiment 6
It is similar, it omits hereby, fish oil can also be wrapped up by zeins.
Embodiment 14
Under normal temperature and pressure, weigh the zeins of 24mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 0.8mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；It weighs simultaneously
The fish oil of 24mg is dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be configured to the fish oil solution of 0.8mg/ml, ultrasound is stirred
About 10min is mixed, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later,
The mass ratio of fish oil and zeins is 10 ﹕ 10 in gained mixed solution at this time, and gained mixed solution is introduced into prior standard
Completing in the culture dish of masking foil for getting ready spontaneously dries.Gained zeins-fish oil powder is cleaned three times with petroleum ether
To remove the not wrapped fish oil on powder surface, gained zeins-fish oil powder FTIR spectrum figure and embodiment 6
It is similar, it omits hereby, fish oil can also be wrapped up by zeins.
Embodiment 15
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；Weigh 9mg's simultaneously
Fish oil is dissolved in the 80%(v/v of 9ml) ethanol-water solution in, be configured to the fish oil solution of 1mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 3 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove powder
The not wrapped fish oil on last surface, gained zeins-fish oil powder FTIR spectrum figure is similar to Example 2, special
This is omitted, and fish oil can also be wrapped up by zeins.
Embodiment 16
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；12mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 12ml) ethanol-water solution in, be configured to the fish oil solution of 1mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 4 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove powder
The not wrapped fish oil on last surface, gained zeins-fish oil powder FTIR spectrum figure is similar to Example 1, special
This is omitted, and fish oil can also be wrapped up by zeins.
Embodiment 17
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；15mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 15ml) ethanol-water solution in, be configured to the fish oil solution of 1mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 5 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove powder
The not wrapped fish oil on last surface, gained zeins-fish oil powder FTIR spectrum figure is similar to Example 3, special
This is omitted, and fish oil can also be wrapped up by zeins.
Embodiment 18
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；18mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 18ml) ethanol-water solution in, be configured to the fish oil solution of 1mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 6 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove powder
The not wrapped fish oil on last surface, gained zeins-fish oil powder FTIR spectrum figure is similar to Example 4, special
This is omitted, and fish oil can also be wrapped up by zeins.
Embodiment 19
Under normal temperature and pressure, weigh the zeins of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in,
It is configured to the zein solution of 1mg/ml, about 10min is stirred by ultrasonic, until solution homogeneous transparent；24mg is weighed simultaneously
Fish oil, be dissolved in the 80%(v/v of 24ml) ethanol-water solution in, be configured to the fish oil solution of 1mg/ml, ultrasonic agitation is about
10min, until solution homogeneous transparent.Gained zein solution is mixed with gained fish oil solution immediately later, at this time institute
Obtaining the mass ratio of fish oil and zeins in mixed solution is 8 ﹕ 10, and gained mixed solution is introduced into preprepared paving
It is spontaneously dried in the culture dish of good masking foil.Gained zeins-fish oil powder is cleaned three times with petroleum ether to remove powder
The not wrapped fish oil on last surface, gained zeins-fish oil powder FTIR spectrum figure is similar to Example 5, special
This is omitted, and fish oil can also be wrapped up by zeins.
The method provided by the present invention using zeins package fish oil is described in detail above.It needs
, it is noted that content described in specific embodiment is preferred embodiment, the present invention for the better implementation present invention
Protection scope be not limited to technical solution described in above embodiment, and should be with the substantive content described in claims
Standard, any possible composition, content and technologic change belong to this without departing from the substantive content of the claims in the present invention
Invent protected range.
Claims (4)
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