CN104758008A - Biological tissue puncture device and method - Google Patents

Abstract

The invention provides a biological tissue puncture device and a method, wherein the device comprises: a first tube and a second tube. This first body includes: a syringe, a piston, and a first needle; wherein a medicament is filled in the syringe. This second body includes: a drawing end, a sampling tube and a second needle; wherein, this second syringe needle is double needle type, and it includes: a capturing end and a collecting end. The collection end of the second needle head located in the extraction end pierces through the sampling tube with sterility and negative pressure, and a sample of the sampled tissue is captured by the sampling tube through the capture end of the second needle head in a pressure difference manner. The medicament in the injection tube can be injected before the first needle head and the second needle head penetrate into the surface of the sampled tissue and before the first needle head and the second needle head are extracted out of the sampled tissue and are separated from the body surface, so that the risk of diffusion infection and even transfer of pathological cells is avoided.

Description

Biological tissue puncture device and method

technical field

The present invention relates to a biological tissue puncture device, especially a double-needle tube combined with a double-needle method. While one needle is used to collect specimens in the body, the other needle can be drawn out before puncturing into the surface of the tissue to be sampled. Continuous injection of a drug after the sampled tissue is removed from the body surface, so that the needle of the sampled sample will not further spread and infect other normal tissues in the body when the needle of the sampled sample is withdrawn from the body surface.

Background technique

In today's medicine, the definitive diagnosis of tumor or cancer requires pathological tissue or cytological examination to determine whether the patient's current condition is suitable for surgical treatment. For pathological specimen tissues, pathological tissue slices or bacterial culture analysis of pathological specimens are further carried out, and the method of guiding fine needle puncture is usually under abdominal ultrasound, but if the tumor is difficult to detect under ultrasound, computer Tomography to guide fine needle aspiration.

It is more difficult to judge the benign and malignant tumors by fine-needle aspiration cytology than slices, so the experience of general pathologists determines whether it is suitable to use fine-needle aspiration cytology to diagnose patients. When a suspicious mass is found by ultrasound, further fine-needle aspiration of tumor cells is required for cytological examination to identify benign and malignant tumors. Currently, fine-needle aspiration cytology is the most effective and accurate test for diagnosing thyroid cancer. General reports in the literature use fine-needle aspiration cytology to judge tumors with a sensitivity of about 80% and a specificity of 100%. Its disadvantage is that it cannot distinguish between carcinoma in situ and invasive carcinoma, so the current use of fine needle aspiration cytology is mostly used in screening patients or detecting metastatic lesions.

However, the current method of collecting samples of cancer tumor cells by fine needle aspiration only stays on the general principle of continuously maintaining negative pressure in ordinary fine needles, so as to directly puncture into the sampled tissue and quickly extract the samples. Pull out the fine needle, but there are no additional protective measures and devices in the process of collecting the sample, so as to further prevent the leakage of the sampled tissue from spreading to the surrounding normal tissue caused by the extraction of the fine needle, resulting in the The normal tissue around the tissue has the possibility of being contaminated by the virus and expanding its scope of infection, further increasing the risk of cancer germ transfer.

Taking liver tumors (liver cancer) as an example, in order to diagnose liver tumors in detail, the pathological tissue of liver tumor cells is often obtained by liver puncture, and correct treatment can be done after further diagnosis is established. Although the probability is not high, there is a considerable probability of occurrence. In view of this, the present invention uses a biological tissue puncture device to reduce the risk of infection of surrounding normal tissues caused by the process of extracting samples from the sampled tissue, and then provides another low-risk puncture sampling method in the medical field. choose.

Contents of the invention

The main purpose of the present invention is to provide a biological tissue puncture device and method, which uses double needle tubes and double needle heads to collect specimens in the body with one needle tube, and the other needle tube can be used before puncturing into the sampled tissue and Injecting a medicament after the sampled tissue is withdrawn and before it leaves the body surface further achieves the purpose of preventing infection of surrounding normal tissues during the process of extracting the sampled tissue sample.

In order to achieve the above object, the technical scheme adopted in the present invention is:

A biological tissue puncture device, characterized in that it comprises:

A first tube body, including: a syringe, a piston and a first needle; and

A second tube body, including: a sampling tube and a second needle;

Wherein, a medicament can be filled in the injection tube, and the sampling tube is used for collecting the sample picked up by the second needle.

Wherein, the first needle and the second needle are parallel needles.

Wherein, the second needle is a double-needle type, which includes: a pick-up end and a collection end.

Wherein, the second tube body also includes an extraction end; the sampling tube is an independent tube body, which includes: a cover and an accommodating space; wherein, the sampling tube is combined with the extraction end of the second tube body end, the collecting end of the second needle pierces through the cover and penetrates into the accommodating space.

Wherein, the cover body is made of soft rubber material with high adhesion, and covers the sampling tube to form a negative pressure state in the accommodating space.

Wherein, the first pipe body and the second pipe body are integrally formed.

Wherein, the medicament is one of cancer-killing medicaments or cancer-cell inhibitors capable of killing cancer cells.

Wherein, an outer surface of the first needle has a plurality of side holes penetrating through an inner wall of a tube of the first needle to provide injection of the medicine.

Wherein, the first needle and the second needle are concentric needles, and the second needle is located inside the first needle and on the same central axis, and an outer surface of the second needle and an outer surface of the first needle The inner walls of the tube are separated by a predetermined distance to provide the drug injection.

In order to achieve the above object, the technical scheme adopted in the present invention is:

A method for using a biological tissue puncture device, comprising the following steps:

(A) Provide a biological tissue puncture device, which includes: a first tube body and a second tube body, the first tube body includes: an injection tube, a piston, and a first needle; the second tube body It includes: a sampling tube and a second needle; wherein, a medicament is filled in the injection tube;

(B) Extending the first needle of the first tube and the second needle of the second tube into the integrated watch at the same time;

(C) making the first needle and the second needle gradually approach the surface of a sampled tissue, pushing the piston of the first needle to inject the medicament in the injection tube first;

(D) puncturing at least the second needle into the sampled tissue while the first needle stops injecting the agent;

(E) Combining the sampling tube into an extraction end of the second tube body, piercing a cover on the sampling tube with a collection end of the second needle located in the extraction end, and penetrating into the sampling tube In an accommodating space with a negative pressure, the diseased cell specimen of the sampled tissue is captured through an extraction end of the second needle into the accommodating space of the sampling tube through a pressure difference;

(F) When at least the second needle is withdrawn from the surface of the sampled tissue, the injection tube is continuously injected with the drug until the first needle and the second needle are withdrawn from the surface of the body and then stopped The drug is discontinued for injection; and

(G) taking out the sampling tube in which the diseased cell specimen of the sampled tissue is extracted from the extraction end of the second tube body.

By means of the collection end of the second needle in the extraction end piercing through the sampling tube with sterile vacuum and negative pressure, the sampling tube is extracted through the extraction end of the second needle in a pressure differential manner. Specimen of sampled tissue. The medicament in the injection tube can be injected before the first needle and the second needle penetrate into the sampled tissue and after being drawn out of the sampled tissue and before leaving the body surface, so as to avoid the risk of spread infection or even transfer of germs.

Description of drawings

Fig. 1 is a schematic cross-sectional view of the first preferred embodiment of the biological tissue puncture device of the present invention;

Fig. 2 is a schematic diagram of the implementation state of the first preferred embodiment of the biological tissue puncture device of the present invention;

Fig. 3 is a flow chart of the steps of the method of using the biological tissue puncture device in the first preferred embodiment of the present invention;

Fig. 4 is a partial enlarged schematic view of the second preferred embodiment of the biological tissue puncture device of the present invention;

Fig. 5 is a partial cross-sectional schematic diagram of a third preferred embodiment of the biological tissue puncture device of the present invention;

Fig. 6 is a schematic cross-sectional view of a third preferred embodiment of the biological tissue puncture device of the present invention.

Explanation of reference signs: 1, 1a～biological tissue puncture device; 11, 11a～first tube body; 111, 111a～injection tube; 112, 112a～piston; 1121a～hollow part; 1122a～piston head; 113, 113a～ 1131～outer surface; 1132, 1132a～tube inner wall; 1133, 1133a～side hole; 12, 12a～second tube body; 121～extraction end; 122, 122a～sampling tube; 1221～cover ;; 1222, 1222a～accommodating space; 1223a～plug body; 123, 123a～second needle; 1231, 1231a～extracting end; 1232～collecting end; 1233a～outer surface; 3～medicine; 5～central axis; 8～culture solution; 91～sampled tissue; 92～body surface; 93～normal tissue; 94～specimen; 95～area.

Detailed ways

Please refer to FIG. 1 and FIG. 2 . FIG. 1 and FIG. 2 are respectively a schematic cross-sectional view and a schematic view of an implementation state of a first preferred embodiment of the biological tissue puncture device of the present invention. In the first preferred embodiment of the present invention, the biological tissue puncture device 1 includes: a first tubular body 11 and a second tubular body 12 . The first tube body 11 further includes: an injection tube 111 , a piston 112 , and a first needle 113 . The second tube body 12 also includes: a extraction end 121 , a sampling tube 122 , and a second needle 123 . The first needle 113 communicates with the injection tube 111. In addition, the second needle 123 is a double-needle type, which includes a extraction end 1231 and a collection end 1232; wherein the collection end 1232 is located at the extraction end. Inside the extraction end 121, and collect the specimen sample through the extraction end 1231. A medicament 3 can be filled in the injection tube 111, and the medicament 3 can be specifically aimed at killing cancer cells but not harming general human tissue cells at present. one of the agents. In this embodiment, the first needle 113 and the second needle 123 are parallel needles, and the injection tube 111 of the first tube 11 and the extraction end 121 of the second tube 12 are integrally formed.

The sampling tube 122 of the second tube body 12 is an independent tube body, which includes: a cover body 1221 and an accommodating space 1222 . The cover 1221 is made of soft rubber material with a high degree of adhesion, and covers the sampling tube 122 to form a sterile vacuum and a negative pressure state in the accommodating space 1222 . The sampling tube 122 can be inserted into the extracting end 121 combined with the second tube body 12, and can penetrate through the collecting end 1232 of the second needle 123 located in the extracting end 121 after a certain pressure is applied. The cover body 1221 allows the collection end 1232 to enter the accommodating space 1222 of the sampling tube 122, and a sample is extracted through the sampling tube 122 through the extraction end 1231 of the second needle 123 in a pressure differential manner. A sample of tissue 91 (tumor or cancer cell population or diseased cell population). The medicament 3 in the injection tube 111 can be used during the period when the first needle 113 and the second needle 123 penetrate into the body surface 92 (such as the visceral surface layer or the human skin surface layer) but before the puncture into the sampled tissue 91 surface, And during the period after being extracted from the surface of the sampled tissue 91 and before breaking away from the body surface 92 (visceral surface or skin surface), the injection is continued, so that the sampled tissue can be separated from the body surface 92 (such as the internal organ surface or human skin surface) A region where the drug 3 is injected is formed between the surfaces of the 91, so as to avoid the diseased cells (such as cancer cells) of the sampled tissue specimen 94 or the sampled tissue 91 being picked up by the second needle 123 The diseased cells contaminated on the end 1231, or the diseased cells leaked from the wound caused by the sampling puncture spread and infect the surrounding normal tissue 93, which may cause the risk of cancer cell metastasis to other organs.

In other words, in the first preferred embodiment of the biological tissue puncture device of the present invention, the first needle head 113 of the first tube body 11 is adjacent to the side of the second needle head 123 of the second tube body 12 . When extracting the specimen in the sampled tissue 91 , the first needle 113 and the second needle 123 puncture the body surface 92 at the same time, pass through the normal tissue 93 and reach the surface of the sampled tissue 91 (not yet punctured into the sampled tissue), push the plunger 112 of the first tube 11 in advance so that the medicament 3 filled in the injection tube 111 can be injected protectively through the first needle 113, so as to reduce the Leakage of diseased cells caused by the pick-up end 1231 of the second needle 123 when puncturing the sampled tissue 91 spreads to the surrounding normal tissue 93, or the second needle 123 is pulled out during the process of pulling out the second needle 123. The possibility of diseased cells contaminated on the outer surface of the pick-up end 1231 spreading to the surrounding normal tissue 93, and because the property of the drug 3 only targets diseased cells or cancer cells and bacteria for killing and protection, it will not harm normal cells relatively Therefore, when the first needle 113 and the second needle 123 puncture into the sampled tissue 91 to sample the sample, the cells or cancer cells that may cause lesions in the sampled tissue 91 (tumor) can be greatly reduced. The puncture poses a risk of leakage infection to the surrounding normal tissue 93 .

When waiting for the first needle 113 and the second needle 123 to enter into the sampled tissue 91, the medicine 3 in the injection tube 111 stops injecting immediately and uses the extracting end 1231 of the second needle 123 to perform the injection. The collection operation of the diseased cells in the sampled tissue 91 (tumor) is to suck the diseased cells in the sampled tissue 91 (tumor) through the sampling tube 122 by means of pressure difference and the extraction by the second needle 123 end 1231 to extract the diseased cell tissue in the sampled tissue 91, and the collection end 1232 pierces through the cover 1221 and enters the accommodating space 1222 in the sampling tube 122 for a preset number of samples Collection operation of the tissue sample 94 (cancer cells or diseased cells).

Immediately afterwards, when the first needle 113 and the second needle 123 are withdrawn from the surface of the sampled tissue 91, push the plunger 112 to continuously inject the medicine 3 into the injection tube 111 until the first needle 113 And after the second needle 123 is withdrawn from the original puncture path and returned to the outside of the body surface 92, then stop pushing the piston 112 to stop the injection of the medicine 3 in the injection tube 111, and collect the sampled tissue. The sampling tube 122 of the body 94 is taken out from the withdrawing end 121 of the second tube body 12 , so that the collecting end 1232 of the second needle 123 is drawn out of the sampling tube 122 .

In this way, not only the sample collection operation of the sampled tissue sample 94 is completed, but also the biological tissue puncture device 1 of the present invention is used to prevent the first needle 113 and the second needle 123 from being separated after the sampled tissue 91 is sampled. The risk of diseased cells (cancer cells) in the sampled tissue 91 spreading and infecting from the sampled tissue 91 to surrounding normal tissues 93 or even transferring to other organs in the path before the body surface 92 is pulled out.

Please refer to FIG. 3 , which is a flowchart of the steps of the method for using the biological tissue puncture device according to the first preferred embodiment of the present invention. The method for using the biological tissue puncture device of the present invention includes the following steps during implementation:

(A) filling an injection tube of a first tube body of the biological tissue puncture device with a predetermined dose of a medicament;

(B) Extending a first needle of the first tube and a second needle of the second tube into the body surface at the same time;

(C) When the first needle and the second needle are close to the surface of a sampled tissue, pushing a piston of the first needle to inject the medicine in the injection tube first;

(D) When the first needle and the second needle penetrate into the sampled tissue, the first needle immediately stops injecting the drug;

(E) Combining a sampling tube into an extraction end of the second tube body, piercing a cover on the sampling tube with a collection end of the second needle located in the extraction end, and penetrating into the sampling tube In an accommodating space with a sterile vacuum and negative pressure effect, the diseased cell specimen of the sampled tissue is extracted through an extraction end of the second needle through a pressure difference;

(F) When the first needle and the second needle are pulled out of the surface of the sampled tissue, push the piston to keep injecting the medicine in the injection tube until the first needle and the second needle are pulled out Immediately stop pushing the plunger outside the body surface to stop the injection of the medicament in the syringe; and

(G) Take out the sampling tube in which the diseased cell specimen of the sampled tissue is extracted from the extraction end of the second tube body, and pull the collecting end of the second needle away from the cover of the sampling tube body.

In other preferred embodiments of the present invention described below, because most of the elements are the same or similar to the foregoing embodiments, the same elements and structures will not be described in detail below, and the same elements will be directly given the same name and Number, and give the same name to similar components, but add an English letter after the original number to distinguish and will not be repeated, and will be described first.

Please refer to FIG. 4 , which is a partially enlarged schematic diagram of a second preferred embodiment of the biological tissue puncture device of the present invention. The difference between the biological tissue puncture device of the second preferred embodiment of the present invention and the first preferred embodiment described in FIGS. 1 and 2 is that an outer surface 1131 of the first needle 113 has a A plurality of side holes 1133 in a tube inner wall 1132 of a needle 113 provide injection of the medicine 3 . That is to say, through the plurality of side holes 1133 provided on the first needle 113, when injecting the medicine 3, the space for protecting and blocking the spread of diseased cells can be further increased, and the capture of the second needle 123 can be guaranteed to be reduced. The possibility of leakage and diffusion of diseased cells caused by the sampling end 1231 when the specimen of the sampled tissue 91 is extracted.

Please refer to FIG. 5 and FIG. 6 , which are a partial cross-sectional view and a schematic cross-sectional view of a third preferred embodiment of the biological tissue puncture device of the present invention, respectively. The difference between the biological tissue puncture device of the third preferred embodiment of the present invention and the second preferred embodiment shown in FIG. 4 is that the biological tissue puncture device 1a of the third preferred embodiment of the present invention, wherein, The first needle head 113a and the second needle head 123a are concentric needles, and the pinhole centers of the first needle head 113a and the second needle head 123a are located on the same central axis 5, and the second needle head 123a is located on the first needle head 113a to form a needle-in-needle structure (the second needle 123a is also pierced inside the first needle 113a). The outer surface 1233a of the second needle 123a is spaced a preset distance d from the tube inner wall 1132a of the first needle 113a so that the medicine 3 can pass between the inner surface of the first needle 113a and the outer surface of the second needle 123a. Interstitial injection outflow. In addition, when the biological tissue puncture device 1a of the present invention collects a sample, the extraction end 1231a of the second needle 123a can protrude from the outside of the first needle 113a and enter the sampled tissue sample 94 Carry out the collection action of cancer cells or diseased cells.

Furthermore, in the biological tissue puncture device 1a according to the third preferred embodiment of the present invention, the second tube body 12a can extend into a hollow space 1121a located in the center of the piston 112a, and together with the piston 112a It is arranged inside the first pipe body 11a. The sampling tube 122a of the second tube body 12a is an independent tube body, which includes: a cover body 1221a, an accommodating space 1222a and a plug body 1223a. The cover 1221a is made of soft rubber material with a high degree of adhesion, and covers the sampling tube 122a so that the accommodating space 1222a is in a sterile and negative pressure state. Moreover, in this embodiment, the sampling tube 122a of the second tube body 12a may be pre-filled with an appropriate amount of a culture medium 8 for preserving the collected diseased cells. Since the piston 112a is a hollow tube body in the form of a piston head 1122a that is penetrated at one end (for insertion into the sampling tube 122a) and the other end is flexible, the sampling tube 122a and the sampling tube 122a of the second tube body 12a can be The connected second needle 123a extends into the hollow 1121a of the piston 112a.

When performing a sample sampling operation, firstly, a front end of a first needle 113a of the first tube body 11a is inserted into the body surface 92 . When the first needle 113a is gradually approaching the sampled tissue 91 but has not yet reached the surface of the sampled tissue 91, the plunger 112a pushing the first needle 113a makes the medicine 3 in the injection tube 111a injected first, which can Therefore, an area 95 injected with the drug 3 is formed between the body surface 92 (such as the visceral surface layer or the human skin surface layer) and the sampled tissue 91 surface. When the front end of the first needle head 113a reaches or is about to reach the surface of the sampled tissue 91, then push the second tube body 12a to push the extraction end 1231a of the front end of the second needle head 123a connected to the sampling tube 122a through the The inside of the first needle 113a protrudes, and at the same time, the medicine 3 in the first needle 113a is squeezed through the second needle 123a through the plurality of side holes 1133a provided on the first needle 113a. The medicine 3 in the first needle 113a is squeezed out from the side holes 1133a, and the pick-up end 1231a of the second needle 123a is further protruded out of the first needle 113a, and penetrates into the sample to be sampled. In the tissue 91 (but at this time, the front end of the first needle 113a still stays on the outer surface of the sampled tissue 91 and will not penetrate). By virtue of the tail end (that is, the collection end) of the second needle 123a piercing through the cover 1221a and protruding into the accommodating space 1222a of the sampling tube 122a, through the negative pressure state inside the sampling tube 122a, to Through the extraction end 1231a of the second needle 123a, the diseased cell sample of the sampled tissue 91 (tumor or cancer cell group or diseased cell group) is sucked and extracted by means of pressure difference into the accommodating space inside the sampling tube 122a 1222a and soaked in the culture solution 8 to complete the action of collecting cancer cells or diseased cells from the sample. Next, extract the sampling tube 122a with the specimen of the sampled tissue 91 out of the hollow part 1121a of the piston 112a, and then extract the piston 112a in an appropriate amount so that the second needle 123a is extracted together with the piston 112a, Until the extraction end 1231a of the second needle 123a is retracted into the front end of the first needle 113a again, then the first tube 11a and the first needle 113a are gradually pulled away from the surface of the sampled tissue 91. At this time, you can choose Squeeze out a small amount of the drug 3 in the first needle 113a again from the side holes 1133a (in order to ensure the anticancer effect of the area 95 of the drug 3), until the first needle 113a is completely withdrawn 92 outside the body surface. Certainly, in the process of gradually withdrawing the first tube body 11a and the first needle 113a from the surface of the sampled tissue 91 but not out of the body surface 92 as described in this embodiment, it is also unnecessary to inject again The medicine 3, because in this embodiment, the extraction end 1231a of the second needle 123a is completely retracted in the first needle 113a and soaked in the original medicine 3 in the first needle 113a, so There is no fear of spreading the diseased cells contaminated on the outer surface of the picking end 1231a of the second needle 123a to other parts.

Furthermore, the biological tissue puncture device 1a of the third preferred embodiment of the present invention includes the following steps during implementation:

(1) Fill a syringe of a first tubular body of the biological tissue puncture device with a preset dose of a drug in a syringe of the third preferred embodiment, and fill a sampling tube of a second tubular body with a preset dose a culture medium;

(2) The second tube extends into a hollow of a piston located in the first tube, and a first needle of the first tube extends into the body surface;

(3) When the first needle is close to the surface of a sampled tissue, pushing the piston of the first needle makes the medicine in the injection tube injected first, so that it can be injected on the surface of the body (such as the surface of internal organs or the surface of human skin) ) and the surface of the sampled tissue form a region where the agent is injected;

(4) Pushing the second tube to protrude the pick-up end of the second needle front end connected to the sampling tube through the inside of the first needle, and at the same time, release the drug in the first needle from the Extruding the medicament in the first needle from the side holes during the extrusion process of the second needle through the plurality of side holes provided on the first needle;

(5) Extend the pick-up end of the second needle out of the first needle and penetrate into the sampled tissue. At this time, the front end of the first needle still stays on the outer surface of the sampled tissue and will not penetrate ;

(6) With the tail end (that is, the collection end) of the second needle a piercing through the cover and protruding into the accommodating space of the sampling tube, through the negative pressure state of the sampling tube part, the pressure The worst way is to draw and extract the diseased cell specimen of a sampled tissue (tumor or cancer cell group or diseased cell group) through the extraction end of the second needle into the storage space inside the sampling tube and soak it in the culture medium. in liquid; and

(7) Extracting the sampling tube from which the sampled tissue is taken out of the hollow of the piston, and then pulling out an appropriate amount of the piston so that the second needle is pulled out together with the piston until the extraction of the second needle The taking end is retracted into the inside of the front end of the first needle again, and then the first tube and the first needle are pulled out of the body surface.

To sum up, the biological tissue puncture device 1 of the present invention includes: a first tubular body 11 and a second tubular body 12 . The first tube body 11 includes: an injection tube 111 , a piston 112 , and a first needle 113 ; wherein, a medicine 3 can be filled in the injection tube 111 . . The second tube body 12 includes: an extraction end 121, a sampling tube 122, and a second needle 123; wherein, the second needle 123 is a double-needle type, which includes: an extraction end 1231 and a collection end 1232.

The sampling tube 122 of the second tube body 12 is an independent tube body, which includes: a cover body 1221 and an accommodating space 1222 . The cover 1221 is made of soft rubber material with a high degree of adhesion, and covers the sampling tube 122 to form a sterile vacuum and a negative pressure state in the accommodating space 1222 . The collecting end 1232 located in the extracting end 121 by means of the second needle 123 pierces the cover 1221 of the sampling tube 122 and penetrates into the accommodating space 1222 of the sampling tube 122 with a sterile and negative pressure, through the The sampling tube 122 collects a specimen of a sampled tissue 91 located in the integrated watch 92 through the extraction end 1231 of the second needle 123 in a pressure differential manner. The medicament 3 in the injection tube 111 can be separated from the body before the extraction end 1231 of the first needle 113 and the second needle 123 penetrates into the surface of the sampled tissue 91 and is drawn out of the sampled tissue 91. Continuous injection is performed before the surface 92, so as to prevent the sampled tissue 91 from making the sampled tissue in the path before the first needle 113 and the second needle 123 are pulled out of the body surface 92 after picking up the specimen. 91 Leakage of diseased cells (cancer cells) in the surrounding normal tissue 93 and even the risk of metastasis to other organs.

The above description is only illustrative of the present invention, rather than restrictive. Those of ordinary skill in the art understand that many modifications, changes or equivalents can be made without departing from the spirit and scope defined in the claims, but All will fall within the protection scope of the present invention.

Claims (10)

1. biological tissue's sting device, is characterized in that, it comprises:

One first body, comprising: a syringe, a piston and one first syringe needle; And

One second body, comprising: a probe tube and one second syringe needle;

Wherein, a medicament can be filled in this syringe, and this probe tube is used to provide the corpse or other object for laboratory examination and chemical testing collected this second syringe needle and capture.

2. biological tissue according to claim 1 sting device, is characterized in that, this first syringe needle and this second syringe needle are and pricking with needle.

3. biological tissue according to claim 1 sting device, is characterized in that, this second syringe needle is double needle kenel, and it comprises: acquisition end and a collecting terminal.

4. biological tissue according to claim 3 sting device, is characterized in that, this second body also comprises an extraction end; This probe tube is a freestanding bodies, and it includes: a lid and an accommodation space; Wherein, this probe tube is incorporated in this extraction end of this second body, and this collecting terminal of this second syringe needle is punctured this lid and running through in this accommodation space.

5. biological tissue according to claim 4 sting device, is characterized in that, this lid is that the soft rubber material with high adaptation formed, and is covered in this probe tube to make in this accommodation space and forms negative pressure state.

6. biological tissue according to claim 1 sting device, is characterized in that, this first body and this second body are integrally formed.

7. biological tissue according to claim 1 sting device, is characterized in that, this medicament be can kill the killing cancer cell medicament of cancerous cell or inhibition of cancer cell agent one of them.

8. biological tissue according to claim 1 sting device, is characterized in that, an outer surface of this first syringe needle has a plurality of side openings of an inside pipe wall of this first syringe needle through, to provide injection this medicament.

9. biological tissue according to claim 1 sting device, it is characterized in that, this first syringe needle is concentric pin with this second syringe needle, and this second syringe needle to be positioned within this first syringe needle and to be positioned on same central shaft, and inside pipe wall interval one predeterminable range of an outer surface of this second syringe needle and this first syringe needle is to provide this drug injection.

10. a using method for biological tissue's sting device, it comprises following implementation step:

(A) provide biological tissue's sting device, it comprises: one first body and one second body, and this first body comprises: a syringe, a piston and one first syringe needle; This second body comprises: a probe tube and one second syringe needle; Wherein, in this syringe, a medicament is filled with;

(B) this first syringe needle of this first body and this second syringe needle of this second body are inserted in a body surface simultaneously;

(C) make this first syringe needle and this second syringe needle move closer to while a sampled tissue surface, this piston pushing this first syringe needle makes this medicament in this syringe inject in advance;

(D) will at least the puncture of this second syringe needle enter in sampled tissue, and now this first syringe needle stops this medicament of injection;

(E) this probe tube being incorporated into one of this second body extracts in end, the lid that the collecting terminal being positioned at this extraction end by means of this second syringe needle punctures on this probe tube, and run through in the accommodation space in negative pressure in this probe tube, and a sick cell corpse or other object for laboratory examination and chemical testing for this sampled tissue of acquisition is held to enter in this accommodation space of this probe tube by the mode of pressure differential via an acquisition of this second syringe needle;

(F) when will at least this second syringe needle pulls out this sampled tissue surface time, make this medicament of continuous injection in this syringe, until this first syringe needle and this second syringe needle are pulled out body surface outer after stop this medicament to stop injecting immediately; And

(G) this probe tube of a sick cell corpse or other object for laboratory examination and chemical testing for this sampled tissue there is is to take out by this extraction end of this second body acquisition.