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CN104678108A - Detecting card for bone alkaline phosphatase, hemoglobin and glutamic-pyruvic transaminase - Google Patents

Detecting card for bone alkaline phosphatase, hemoglobin and glutamic-pyruvic transaminase Download PDF

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Publication number
CN104678108A
CN104678108A CN201510095141.0A CN201510095141A CN104678108A CN 104678108 A CN104678108 A CN 104678108A CN 201510095141 A CN201510095141 A CN 201510095141A CN 104678108 A CN104678108 A CN 104678108A
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China
Prior art keywords
sample
pad
application
zone
filtrating area
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Pending
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CN201510095141.0A
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Chinese (zh)
Inventor
胡容
王忠亮
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Anhui Hui Bang Bioengineering Inc Co
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Anhui Hui Bang Bioengineering Inc Co
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Priority to CN201510095141.0A priority Critical patent/CN104678108A/en
Publication of CN104678108A publication Critical patent/CN104678108A/en
Pending legal-status Critical Current

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721Haemoglobin
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306Solid-phase reaction mechanisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a detecting card for bone alkaline phosphatase, hemoglobin and glutamic-pyruvic transaminase. The detecting card comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a fluorescent pad, a nitrocellulose membrane, a detecting line, a quality control line, water absorption filter paper, sample feeding ports, sample adding tubes, a support, a stop piece, a sample adding zone, a reaction zone, a sample flowing pad, a bulge, a reaction film and an added sample filtering zone, wherein the sample pad, the nitrocellulose membrane, the fluorescent pad, the water absorption filter paper, the added sample filtering zone, the sample flowing pad, the bulge, the sample adding zone and the reaction zone are arranged on the PVC bottom plate; one end of the sample pad is overlapped on the fluorescent pad, the other end of the fluorescent pad is overlapped on the nitrocellulose membrane, the water absorption filter paper is overlapped at the other end of the nitrocellulose membrane, the detecting line and the quality control line are formed on the nitrocellulose membrane, the added sample filtering zone and the sample flowing pad are contacted, and the sample adding tubes are arranged above the sample pad, the added sample filtering zone and the sample adding zone. The detecting card has the advantages of simplicity, convenience and rapidness in operation and the like.

Description

A kind of NBAP, haemoglobin, glutamic-pyruvic transaminase test card
Technical field
The present invention relates to a kind of NBAP, haemoglobin, glutamic-pyruvic transaminase test card, be applied to medical science external diagnosis reagent field.
Background technology
NBAP (BALP) is a hypotype in alkaline phosphatase isoenzyme, synthesized by Gegenbaur's cell, it mainly concentrates on ossified position, NBAP is the biomacromolecule with catalytic activity of Gegenbaur's cell synthesis, its hetero-organization and organ do not synthesize BALP, for osteogenic tissue is peculiar.BALP activity is directly proportional to the osteoblastic quantity of activity, is the single-minded mark of reflection osteoblast activity and quantity.So its active height is direct, special, the formation that reflects bone delicately with absorb multidate information, be one and there is tissue specificity, cover the full course of disease, not by the biochemical marker that other conditions affect.Children's's NBAP is mainly used in the specificity reference index of Rickets early diagnosis and subclinical discriminating in recent years, adult's NBAP detects for pregnant and lying-in women, pregnant and lying-in women can be instructed in time to replenish the calcium safely and effectively and osteoporosis examination in postpartum.For the elderly, can examination osteoporosis.Rachitic pathogenic process is a chronic process, and primary clinical manifestation does not have specificity, until treating when there is obvious skeleton change.The best period for the treatment of will be missed.Adverse effect is caused to upgrowth and development of children, therefore, in clinical position, needs an index to weigh treatment level.Prevent insufficient therapy or excessive.Simple dependence sings and symptoms easily causes mistaken diagnosis and fails to pinpoint a disease in diagnosis.Blood calcium in the past, phosphorus, whole blood alkaline phosphatase and x-ray detection sensitivity are low, poor specificity.Shall not be applied to early diagnosis.25 (OH) D3 is the reliable index of vitD nutrition condition in internationally recognized antimer. can be used as the index of early diagnosis.This laboratory operating procedures is loaded down with trivial details.Be not suitable for carrying out in primary care health institution.The detection of children's's NBAP, there is the advantages such as easy, quick, special, responsive, the clinical practice of this reagent provides the diagnosis basis of science to early detection rickets, very large clinical meaning is had to guiding clinical treatment, current detection NBAP product is semi-quantitative analysis, and testing result cannot quantitative test.
Haemoglobin (Hb) is erythrocytic principal ingredient, haemoglobin is made up of four chains, article two, α chain and two β chains, each chain has a ring-like heme comprising an iron atom, haemoglobin is a kind of protein being responsible for delivery oxygen in human body, haemoglobin is the most important index diagnosing severity of anemia clinically, also be the hemopathic important indicators of auxiliary diagnosis other types simultaneously, at present, clinical labororatory's many employings Automatic Blood Cell Analyzer Measuring hemoglobin, there is sense cycle long, need a large amount of reagent, carry inconvenience, be not suitable for the quick detection of haemoglobin individual event object quick test beyond laboratory and blood donor's haemoglobin in enormous quantities.Germany produces DE202 portable Hemoglobin Meter, and also to there is microplate volume comparatively large, not easily location and the higher deficiency of use cost.
Glutamic-pyruvic transaminase (ALT) is a kind of non-plasma-specific enzyme, and the dimer be made up of two identical polypeptied chain subunits, molecular weight is 110 kD.Glutamic-pyruvic transaminase is free to be present in cytoplasm, especially the abundantest in liver cell.Inside and outside liver cell, ion difference changes, cell membrane swelling, when the increase of cell hole or meronecrosis, glutamic-pyruvic transaminase can be released in blood in cell, clear or the blood plasma glutamic-pyruvic transaminase of hyperamization significantly increases, therefore glutamic-pyruvic transaminase is the most responsive a kind of Testing index of hepatic disorder by world health organisation recommendations, and current clinical detection glutamic-pyruvic transaminase mainly uses the method for the liquid reagent of Biochemical Analyzer, and the method is consuming time, complicated operation, need than relatively large instrument.
Summary of the invention
For solving the defect of prior art, the invention discloses a kind of energy simultaneous quantitative and detecting NBAP, haemoglobin, glutamic-pyruvic transaminase content, NBAP simple and efficient to handle, haemoglobin, glutamic-pyruvic transaminase test card.
The invention discloses a kind of NBAP, haemoglobin, glutamic-pyruvic transaminase test card, comprising: PVC base plate, sample pad, fluorescence pad, nitrocellulose filter, detection line, nature controlling line, absorbent filter, sample inlet port, application of sample pipe, support, catch, sample application zone, reaction zone, sample flow pad, projection, reaction film, application of sample filtrating area, described PVC base plate is provided with sample pad, nitrocellulose filter, fluorescence pad, absorbent filter, application of sample filtrating area, sample flow pad, projection, sample application zone and reaction zone, described sample pad one end is overlapped on fluorescence pad, the described fluorescence pad other end is overlapped on nitrocellulose filter, described absorbent filter is overlapped on the nitrocellulose filter other end, described sample pad is provided with application of sample pipe, described detection line and nature controlling line are located on nitrocellulose filter, described detection line and nature controlling line are parallel to each other, and detection line is near fluorescence pad end, nature controlling line is away from fluorescence pad end, described detection line and nature controlling line are all coated with protein antibodies, described application of sample filtrating area contacts with sample flow pad, described projection is provided with reaction film, described reaction film is positioned at the top of sample flow pad, described sample application zone is communicated with reaction zone, described reaction zone is provided with blow vent, reaction reagent and the molten reagent of born of the same parents is provided with in described reaction zone, described sample pad, application of sample filtrating area, the top of sample application zone is provided with application of sample pipe, the top ports place of described application of sample pipe is provided with catch, the bottom of the application of sample pipe above described sample pad contacted with sample pad, contact with application of sample filtrating area bottom application of sample pipe above described application of sample filtrating area, the bottom of the application of sample pipe above described sample application zone contacts with sample application zone, the application of sample pipe above described sample pad, application of sample pipe above application of sample filtrating area and the application of sample Guan Jun above sample application zone by support installing on PVC base plate.
Compared with prior art beneficial effect of the present invention is: by above-mentioned setting, the detection of NBAP can be carried out by sample pad, fluorescence pad, nitrocellulose filter, absorbent filter, the detection of glutamic-pyruvic transaminase can be carried out by application of sample filtrating area, sample flow pad and reaction film, the detection of haemoglobin can be carried out by sample application zone and reaction zone, and by the design of application of sample pipe, can operating personnel pass through to application of sample pipe application of sample complete operation, thus the situation that prevents from being scattered occurs, ultimate guarantee Detection results.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of NBAP of the present invention, haemoglobin, glutamic-pyruvic transaminase test card.
Wherein: 1-PVC base plate; 2-sample pad; 3-fluorescence pad; 4-nitrocellulose filter; 5-detection line; 6-nature controlling line; 7-absorbent filter; 8-sample inlet port; 9-application of sample pipe; 10-support; 11-catch; 12-sample application zone; 13-reaction zone; 14-sample flow pad; 15-projection; 16-reaction film; 17-application of sample filtrating area.
Embodiment
As shown in Figure 1, the invention discloses a kind of NBAP, haemoglobin, glutamic-pyruvic transaminase test card, comprising: PVC base plate 1, sample pad 2, fluorescence pad 3, nitrocellulose filter 4, detection line 5, nature controlling line 6, absorbent filter 7, sample inlet port 8, application of sample pipe 9, support 10, catch 11, sample application zone 12, reaction zone 13, sample flow pad 14, projection 15, reaction film 16, application of sample filtrating area 17, described PVC base plate 1 is provided with sample pad 2, nitrocellulose filter 4, fluorescence pad 3, absorbent filter 7, application of sample filtrating area 17, sample flow pad 14, projection 15, sample application zone 12 and reaction zone 13, described sample pad 2 one end is overlapped on fluorescence pad 3, described fluorescence pad 3 other end is overlapped on nitrocellulose filter 4, described absorbent filter 7 is overlapped on nitrocellulose filter 4 other end, described sample pad 2 is provided with application of sample pipe 9, described detection line 5 and nature controlling line 6 are located on nitrocellulose filter 4, described detection line 5 and nature controlling line 6 are parallel to each other, and detection line 5 is near fluorescence pad 3 end, nature controlling line 6 is away from fluorescence pad 3 end, described detection line 5 and nature controlling line 6 are all coated with protein antibodies, described application of sample filtrating area 17 contacts with sample flow pad 14, described projection 15 is provided with reaction film 16, described reaction film 16 is positioned at the top of sample flow pad 14, described sample application zone 12 is communicated with reaction zone 13, described reaction zone 13 is provided with blow vent, reaction reagent and the molten reagent of born of the same parents is provided with in described reaction zone 13, described sample pad 2, application of sample filtrating area 17, the top of sample application zone 12 is provided with application of sample pipe 9, the top ports place of described application of sample pipe 9 is provided with catch 11, the bottom of the application of sample pipe 9 above described sample pad 2 contacted with sample pad 2, contact with application of sample filtrating area 17 bottom application of sample pipe 9 above described application of sample filtrating area 17, the bottom of the application of sample pipe 9 above described sample application zone 12 contacts with sample application zone 12, the application of sample pipe 9 above described sample pad 2, application of sample pipe 9 above application of sample filtrating area 17 and the application of sample pipe 9 above sample application zone 12 are all arranged on PVC base plate 1 by support 10.
Above embodiment only in order to the present invention is described and and unrestricted technical scheme described in the invention; Therefore, although this instructions with reference to each above-mentioned embodiment to present invention has been detailed description, those of ordinary skill in the art should be appreciated that and still can modify to the present invention or equivalent to replace; And all do not depart from technical scheme and the improvement thereof of the spirit and scope of the present invention, it all should be encompassed in right of the present invention.

Claims (1)

1. a NBAP, haemoglobin, glutamic-pyruvic transaminase test card, comprising: PVC base plate, sample pad, fluorescence pad, nitrocellulose filter, detection line, nature controlling line, absorbent filter, sample inlet port, application of sample pipe, support, catch, sample application zone, reaction zone, sample flow pad, projection, reaction film, application of sample filtrating area, is characterized in that: described PVC base plate is provided with sample pad, nitrocellulose filter, fluorescence pad, absorbent filter, application of sample filtrating area, sample flow pad, projection, sample application zone and reaction zone, described sample pad one end is overlapped on fluorescence pad, the described fluorescence pad other end is overlapped on nitrocellulose filter, described absorbent filter is overlapped on the nitrocellulose filter other end, described sample pad is provided with application of sample pipe, described detection line and nature controlling line are located on nitrocellulose filter, described detection line and nature controlling line are parallel to each other, and detection line is near fluorescence pad end, nature controlling line is away from fluorescence pad end, described detection line and nature controlling line are all coated with protein antibodies, described application of sample filtrating area contacts with sample flow pad, described projection is provided with reaction film, described reaction film is positioned at the top of sample flow pad, described sample application zone is communicated with reaction zone, described reaction zone is provided with blow vent, reaction reagent and the molten reagent of born of the same parents is provided with in described reaction zone, described sample pad, application of sample filtrating area, the top of sample application zone is provided with application of sample pipe, the top ports place of described application of sample pipe is provided with catch, the bottom of the application of sample pipe above described sample pad contacted with sample pad, contact with application of sample filtrating area bottom application of sample pipe above described application of sample filtrating area, the bottom of the application of sample pipe above described sample application zone contacts with sample application zone, the application of sample pipe above described sample pad, application of sample pipe above application of sample filtrating area and the application of sample Guan Jun above sample application zone by support installing on PVC base plate.
CN201510095141.0A 2015-03-04 2015-03-04 Detecting card for bone alkaline phosphatase, hemoglobin and glutamic-pyruvic transaminase Pending CN104678108A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106353261A (en) * 2016-08-31 2017-01-25 长沙励思生物技术有限公司 Bone alkaline phosphatase analyzer
CN109085348A (en) * 2017-08-21 2018-12-25 上海荒岛科技有限公司 A kind of method for analyzing body fluid
CN111197071A (en) * 2020-01-10 2020-05-26 杭州联晟生物科技有限公司 Test card for detecting glutamic-pyruvic transaminase by photochemical method and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103412115A (en) * 2013-07-19 2013-11-27 郭宗明 Glutamic-pyruvic transaminase and ferrohemoglobin detection card
CN103412123A (en) * 2013-09-03 2013-11-27 天津朗赛生物科技有限公司 Bone alkaline phosphatase test strip and preparation method thereof
CN203858249U (en) * 2014-05-26 2014-10-01 安徽惠邦生物工程股份有限公司 Novel detection test strip

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103412115A (en) * 2013-07-19 2013-11-27 郭宗明 Glutamic-pyruvic transaminase and ferrohemoglobin detection card
CN103412123A (en) * 2013-09-03 2013-11-27 天津朗赛生物科技有限公司 Bone alkaline phosphatase test strip and preparation method thereof
CN203858249U (en) * 2014-05-26 2014-10-01 安徽惠邦生物工程股份有限公司 Novel detection test strip

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106353261A (en) * 2016-08-31 2017-01-25 长沙励思生物技术有限公司 Bone alkaline phosphatase analyzer
CN109085348A (en) * 2017-08-21 2018-12-25 上海荒岛科技有限公司 A kind of method for analyzing body fluid
CN109085348B (en) * 2017-08-21 2019-07-23 上海荒岛科技有限公司 A kind of method for analyzing body fluid
CN111197071A (en) * 2020-01-10 2020-05-26 杭州联晟生物科技有限公司 Test card for detecting glutamic-pyruvic transaminase by photochemical method and preparation method thereof

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Application publication date: 20150603